AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57259

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57259.t1 MTR_1g016080 93.640 283 14 1 1 283 2 280 1.68e-178 493
MS.gene57259.t1 MTR_3g104420 65.918 267 88 2 19 283 7 272 8.13e-130 369
MS.gene57259.t1 MTR_2g006770 43.404 235 133 0 41 275 17 251 1.20e-66 209
MS.gene57259.t1 MTR_8g089715 40.517 232 135 1 42 270 15 246 2.21e-63 200
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57259.t1 AT5G11870 51.825 274 125 4 11 282 1 269 6.22e-101 296
MS.gene57259.t1 AT5G11870 51.685 267 122 4 11 275 1 262 1.78e-97 287
MS.gene57259.t1 AT1G71190 41.245 257 150 1 25 280 1 257 1.27e-61 196
MS.gene57259.t1 AT1G71190 35.366 246 119 1 35 280 11 216 1.42e-48 161

Find 54 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTAGGAGAGCGTGGAATTT+TGG 0.177744 1.4:-6268173 None:intergenic
CTATTCTATGCTGGAATTAT+AGG 0.182046 1.4:+6268524 MS.gene57259:CDS
TATTCTATGCTGGAATTATA+GGG 0.258418 1.4:+6268525 MS.gene57259:CDS
CGTTATCAGCGGGCACTCTT+TGG 0.268066 1.4:+6269975 MS.gene57259:CDS
GCTGACTTGCGCAATCTTCT+TGG 0.276021 1.4:+6268208 MS.gene57259:CDS
GGAATTATAGGGGTAGCTTT+TGG 0.299826 1.4:+6268536 MS.gene57259:CDS
ACTATACTCACTCAAGATAT+TGG 0.300455 1.4:+6269802 MS.gene57259:CDS
ACAGGGAGCGTAAGTTTAAA+AGG 0.336107 1.4:+6270049 MS.gene57259:CDS
GTGATCACATTCACCGTGTT+GGG 0.344613 1.4:-6268322 None:intergenic
TTCAGAATTGGAGTTTGAAT+TGG 0.355442 1.4:+6268038 MS.gene57259:CDS
GCAGAACTTATGATGATATT+CGG 0.357442 1.4:+6269712 MS.gene57259:intron
GGTTTAGTTCTTACTCTTGA+AGG 0.365060 1.4:+6268376 MS.gene57259:CDS
AGAGAATTGGCCAGCGGATT+GGG 0.369422 1.4:+6269512 MS.gene57259:CDS
AGTCTTGTGGGACACTTTGC+CGG 0.373549 1.4:+6268595 MS.gene57259:CDS
CACAAGACTCTATCGTTATC+AGG 0.387981 1.4:-6268581 None:intergenic
AGTTTGGTTATTGAGAGAAT+TGG 0.409279 1.4:+6269499 MS.gene57259:CDS
TGACGATGATGATTCAGAAT+TGG 0.423301 1.4:+6268026 None:intergenic
TTAGTGTGATGCTCATCTAC+AGG 0.457946 1.4:+6270031 MS.gene57259:CDS
GAGAGAATTGGCCAGCGGAT+TGG 0.461721 1.4:+6269511 MS.gene57259:CDS
TAGCTAAAGGAAGAATGAAC+TGG 0.473251 1.4:-6269749 None:intergenic
TTAGTTCTCAAGCAGAGATA+TGG 0.474235 1.4:+6268492 MS.gene57259:intron
TTCATAAACAACACAAGTAA+AGG 0.483567 1.4:-6269566 None:intergenic
TTTGGGAGAGAAGAGAAAGA+AGG 0.500392 1.4:-6268155 None:intergenic
GATTGCGCAAGTCAGCGAAT+TGG 0.503286 1.4:-6268201 None:intergenic
TTACTTGTGTTGTTTATGAA+CGG 0.506820 1.4:+6269569 MS.gene57259:CDS
TATCGTTATCAGGCTTCAAA+TGG 0.512826 1.4:-6268571 None:intergenic
TGATAACGATAGAGTCTTGT+GGG 0.513805 1.4:+6268583 MS.gene57259:CDS
TGTGATCACATTCACCGTGT+TGG 0.514498 1.4:-6268323 None:intergenic
CTCAAGCAGAGATATGGTCT+TGG 0.517070 1.4:+6268498 MS.gene57259:CDS
CACAAGTAAAGGCTGCGAAA+AGG 0.526322 1.4:-6269555 None:intergenic
TTTGGAACACTTGTGCTTAG+CGG 0.540650 1.4:+6269993 MS.gene57259:CDS
AAGTAAAGGCTGCGAAAAGG+AGG 0.541078 1.4:-6269552 None:intergenic
TGGTGGTGAGTAGGAGAGCG+TGG 0.543307 1.4:-6268181 None:intergenic
TGCGCAAGTCAGCGAATTGG+TGG 0.545924 1.4:-6268198 None:intergenic
GCAGCTGCTGCAATAGCTAA+AGG 0.548300 1.4:-6269762 None:intergenic
TCTTGGATACTATTCTATGC+TGG 0.553325 1.4:+6268515 MS.gene57259:CDS
TCAGCGAATTGGTGGTGAGT+AGG 0.573822 1.4:-6268190 None:intergenic
GCAAATAACTACGTTATCAG+CGG 0.576718 1.4:+6269964 MS.gene57259:CDS
GAATTGGAGTTTGAATTGGA+TGG 0.576928 1.4:+6268042 MS.gene57259:CDS
TGATCACATTCACCGTGTTG+GGG 0.577459 1.4:-6268321 None:intergenic
CAAATAACTACGTTATCAGC+GGG 0.577528 1.4:+6269965 MS.gene57259:CDS
TAGTGTGATGCTCATCTACA+GGG 0.584946 1.4:+6270032 MS.gene57259:CDS
TACAACACATCCCAATCCGC+TGG 0.586145 1.4:-6269522 None:intergenic
TTAGTTCTTACTCTTGAAGG+AGG 0.606045 1.4:+6268379 MS.gene57259:CDS
CTGATAACGATAGAGTCTTG+TGG 0.611073 1.4:+6268582 MS.gene57259:CDS
CTTGAGTGAGTATAGTCAGA+GGG 0.627291 1.4:-6269795 None:intergenic
AGTAAAGGCTGCGAAAAGGA+GGG 0.633786 1.4:-6269551 None:intergenic
ATTCTATGCTGGAATTATAG+GGG 0.637655 1.4:+6268526 MS.gene57259:CDS
TGATAACGTAGTTATTTGCA+CGG 0.644610 1.4:-6269960 None:intergenic
TCTTGAGTGAGTATAGTCAG+AGG 0.667258 1.4:-6269796 None:intergenic
TTTCACAGGTGTCCCCAACA+CGG 0.676395 1.4:+6268309 MS.gene57259:intron
GCACTAATGGCAGATGATGG+TGG 0.681415 1.4:+6269456 MS.gene57259:intron
AACCAAACTAGAGAAAAGTG+AGG 0.710937 1.4:-6269485 None:intergenic
TTATTGAGAGAATTGGCCAG+CGG 0.722249 1.4:+6269506 MS.gene57259:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTAATTTTTCTTAATT+TGG + chr1.4:6268266-6268285 MS.gene57259:intron 10.0%
!! ATAAATATATTCATATAGAG+AGG - chr1.4:6268644-6268663 None:intergenic 15.0%
!!! AACTATTTCTATATTTGAAT+AGG + chr1.4:6269056-6269075 MS.gene57259:intron 15.0%
!!! ACTATTTCTATATTTGAATA+GGG + chr1.4:6269057-6269076 MS.gene57259:intron 15.0%
!!! TTAATTTTTCTTAATTTGGA+TGG + chr1.4:6268270-6268289 MS.gene57259:intron 15.0%
!!! TTTAAATGTTTTATACTTTG+AGG + chr1.4:6269638-6269657 MS.gene57259:intron 15.0%
!! AAACTAAAATAAGTCAATCA+GGG - chr1.4:6270483-6270502 None:intergenic 20.0%
!! AATAAGCTAAAAATAGCTTA+TGG + chr1.4:6268937-6268956 MS.gene57259:intron 20.0%
!! AGGAAACATTAATTACAAAA+AGG - chr1.4:6270777-6270796 None:intergenic 20.0%
!! CTTATAAAGAGAAAATGTTA+AGG + chr1.4:6269325-6269344 MS.gene57259:intron 20.0%
!! TAAACTAAAATAAGTCAATC+AGG - chr1.4:6270484-6270503 None:intergenic 20.0%
!! TCTGTCATAATTTAAACAAA+TGG - chr1.4:6269297-6269316 None:intergenic 20.0%
!!! ATGACATTTCAAATTTTTGA+AGG - chr1.4:6270226-6270245 None:intergenic 20.0%
!!! ATTGTTTTCATAAGATATCT+TGG - chr1.4:6270611-6270630 None:intergenic 20.0%
!!! CTATTTCTATATTTGAATAG+GGG + chr1.4:6269058-6269077 MS.gene57259:intron 20.0%
!!! TAAGCTATTTCAATAATGTA+AGG + chr1.4:6268789-6268808 MS.gene57259:intron 20.0%
!!! TAATTTTAGCATAAACTTAG+TGG - chr1.4:6269675-6269694 None:intergenic 20.0%
!!! TTTTGTAATTAATGTTTCCT+TGG + chr1.4:6270777-6270796 MS.gene57259:intron 20.0%
! AACTAAAATAAGTCAATCAG+GGG - chr1.4:6270482-6270501 None:intergenic 25.0%
! ATAAAGAGAAAATGTTAAGG+TGG + chr1.4:6269328-6269347 MS.gene57259:intron 25.0%
! ATATATTCATATAGAGAGGT+AGG - chr1.4:6268640-6268659 None:intergenic 25.0%
! GATAAAAGTAACTAACCTTA+CGG - chr1.4:6268413-6268432 None:intergenic 25.0%
! TAAAATTATGATCTGCTAGT+TGG + chr1.4:6269687-6269706 MS.gene57259:intron 25.0%
! TATTCTATGCTGGAATTATA+GGG + chr1.4:6268525-6268544 MS.gene57259:CDS 25.0%
! TGTGATTAGATTCTAATATG+AGG + chr1.4:6268453-6268472 MS.gene57259:intron 25.0%
! TTCATAAACAACACAAGTAA+AGG - chr1.4:6269569-6269588 None:intergenic 25.0%
!! TTCTGAAGAAAAAAACTAGT+GGG - chr1.4:6270249-6270268 None:intergenic 25.0%
!! TTTTCATAAGATATCTTGGA+GGG - chr1.4:6270607-6270626 None:intergenic 25.0%
!!! AAATTTCCCATTTTTAGTTG+TGG + chr1.4:6268345-6268364 MS.gene57259:CDS 25.0%
!!! AATTTCCCATTTTTAGTTGT+GGG + chr1.4:6268346-6268365 MS.gene57259:CDS 25.0%
!!! AGATATTGGTTTTCATCAAT+AGG + chr1.4:6269816-6269835 MS.gene57259:CDS 25.0%
!!! TTACTTGTGTTGTTTATGAA+CGG + chr1.4:6269569-6269588 MS.gene57259:CDS 25.0%
!!! TTTCTTAATTTGGATGGTTT+TGG + chr1.4:6268276-6268295 MS.gene57259:intron 25.0%
AAAACGAACTAAGTACTAAG+AGG - chr1.4:6269408-6269427 None:intergenic 30.0%
AACCAAAAATTCCATCGAAT+AGG - chr1.4:6270089-6270108 None:intergenic 30.0%
AAGCTTGAGACTATTATTAG+AGG - chr1.4:6269609-6269628 None:intergenic 30.0%
ACCAATGAGCTTCTTAATAA+AGG - chr1.4:6269435-6269454 None:intergenic 30.0%
ACTATACTCACTCAAGATAT+TGG + chr1.4:6269802-6269821 MS.gene57259:CDS 30.0%
AGCATCACACTAAGTAAAAA+CGG - chr1.4:6270024-6270043 None:intergenic 30.0%
AGTTTGGTTATTGAGAGAAT+TGG + chr1.4:6269499-6269518 MS.gene57259:CDS 30.0%
ATAAATGTTGCAAAAGTCTG+AGG - chr1.4:6269849-6269868 None:intergenic 30.0%
ATGCTATAAGCACTTAATTG+TGG + chr1.4:6270718-6270737 MS.gene57259:intron 30.0%
ATTATAGAAGAGAGCTTATG+TGG - chr1.4:6269097-6269116 None:intergenic 30.0%
ATTCTATGCTGGAATTATAG+GGG + chr1.4:6268526-6268545 MS.gene57259:CDS 30.0%
CAATAATGTAAGGTCTAGTT+TGG + chr1.4:6268799-6268818 MS.gene57259:intron 30.0%
CAATGAGCTTCTTAATAAAG+GGG - chr1.4:6269433-6269452 None:intergenic 30.0%
CCAATGAGCTTCTTAATAAA+GGG - chr1.4:6269434-6269453 None:intergenic 30.0%
CCTAATCAAAAACCAATTCA+TGG - chr1.4:6270376-6270395 None:intergenic 30.0%
CTATTCTATGCTGGAATTAT+AGG + chr1.4:6268524-6268543 MS.gene57259:CDS 30.0%
GCAGAACTTATGATGATATT+CGG + chr1.4:6269712-6269731 MS.gene57259:intron 30.0%
TTAAACAAATGGAGCAACTA+GGG - chr1.4:6269286-6269305 None:intergenic 30.0%
TTCAGAATTGGAGTTTGAAT+TGG + chr1.4:6268038-6268057 MS.gene57259:CDS 30.0%
TTCTTTAGCCAAAACAATCA+TGG - chr1.4:6268711-6268730 None:intergenic 30.0%
TTGTGAAACCAAATTTCACA+AGG - chr1.4:6270112-6270131 None:intergenic 30.0%
TTTAAACAAATGGAGCAACT+AGG - chr1.4:6269287-6269306 None:intergenic 30.0%
! AACCTATTCGATGGAATTTT+TGG + chr1.4:6270084-6270103 MS.gene57259:intron 30.0%
! ATTTTTACCTCACCATGAAT+TGG + chr1.4:6270361-6270380 MS.gene57259:intron 30.0%
! CTTCTGAAGAAAAAAACTAG+TGG - chr1.4:6270250-6270269 None:intergenic 30.0%
! GTTTTCATAAGATATCTTGG+AGG - chr1.4:6270608-6270627 None:intergenic 30.0%
! TGATAACGTAGTTATTTGCA+CGG - chr1.4:6269963-6269982 None:intergenic 30.0%
! TTCCTTTTCCATGATTGTTT+TGG + chr1.4:6268700-6268719 MS.gene57259:intron 30.0%
! TTTGGTTTCACAAATTGTTC+TGG + chr1.4:6270119-6270138 MS.gene57259:intron 30.0%
! TTTTCAAAACAGCTTGACTT+TGG - chr1.4:6268895-6268914 None:intergenic 30.0%
!! TATGAGGTGTGTTTTTGATT+TGG + chr1.4:6268469-6268488 MS.gene57259:intron 30.0%
!!! AGTGTTTTAAAGGGCTTATT+TGG - chr1.4:6269263-6269282 None:intergenic 30.0%
!!! CCATGAATTGGTTTTTGATT+AGG + chr1.4:6270373-6270392 MS.gene57259:intron 30.0%
!!! TTTTGGTTCCTTGTGAAATT+TGG + chr1.4:6270101-6270120 MS.gene57259:intron 30.0%
AACCAAACTAGAGAAAAGTG+AGG - chr1.4:6269488-6269507 None:intergenic 35.0%
AGAACACCCACAACTAAAAA+TGG - chr1.4:6268355-6268374 None:intergenic 35.0%
AGCCAAAACAATCATGGAAA+AGG - chr1.4:6268705-6268724 None:intergenic 35.0%
AGCCTAGACAAACTAGAATT+TGG - chr1.4:6270279-6270298 None:intergenic 35.0%
CAAATAACTACGTTATCAGC+GGG + chr1.4:6269965-6269984 MS.gene57259:CDS 35.0%
CAATAACACTTGTGTGAGTT+TGG - chr1.4:6269224-6269243 None:intergenic 35.0%
CATATAGAGAGGTAGGTAAT+GGG - chr1.4:6268633-6268652 None:intergenic 35.0%
CCCTTTATTAAGAAGCTCAT+TGG + chr1.4:6269431-6269450 MS.gene57259:intron 35.0%
CTATATTTGAATAGGGGATG+CGG + chr1.4:6269064-6269083 MS.gene57259:intron 35.0%
GAACACCCACAACTAAAAAT+GGG - chr1.4:6268354-6268373 None:intergenic 35.0%
GAATTGGAGTTTGAATTGGA+TGG + chr1.4:6268042-6268061 MS.gene57259:CDS 35.0%
GCAAATAACTACGTTATCAG+CGG + chr1.4:6269964-6269983 MS.gene57259:CDS 35.0%
GCATCACACTAAGTAAAAAC+GGG - chr1.4:6270023-6270042 None:intergenic 35.0%
GTCTTGTGGATGTTTGAATT+TGG - chr1.4:6270191-6270210 None:intergenic 35.0%
TAGCTAAAGGAAGAATGAAC+TGG - chr1.4:6269752-6269771 None:intergenic 35.0%
TATCGTTATCAGGCTTCAAA+TGG - chr1.4:6268574-6268593 None:intergenic 35.0%
TCAAAAACCAATTCATGGTG+AGG - chr1.4:6270371-6270390 None:intergenic 35.0%
TCATATAGAGAGGTAGGTAA+TGG - chr1.4:6268634-6268653 None:intergenic 35.0%
TCTTGGATACTATTCTATGC+TGG + chr1.4:6268515-6268534 MS.gene57259:CDS 35.0%
! CTATTAGCACGTGTTTGTTT+TGG + chr1.4:6270396-6270415 MS.gene57259:intron 35.0%
! TGATAACGATAGAGTCTTGT+GGG + chr1.4:6268583-6268602 MS.gene57259:CDS 35.0%
! TGATCAAAATCGATTCCTGA+TGG - chr1.4:6270309-6270328 None:intergenic 35.0%
! TTAGTTCTCAAGCAGAGATA+TGG + chr1.4:6268492-6268511 MS.gene57259:intron 35.0%
! TTAGTTCTTACTCTTGAAGG+AGG + chr1.4:6268379-6268398 MS.gene57259:CDS 35.0%
!! GAATTTGGAAAACTGACTTG+AGG - chr1.4:6270176-6270195 None:intergenic 35.0%
!! GGTTTAGTTCTTACTCTTGA+AGG + chr1.4:6268376-6268395 MS.gene57259:CDS 35.0%
!! TATTTGAAGCCTGACCTTTT+TGG + chr1.4:6269883-6269902 MS.gene57259:intron 35.0%
!! TTCCTCACTTTTCTCTAGTT+TGG + chr1.4:6269483-6269502 MS.gene57259:CDS 35.0%
!! TTGATGATGAACCTATTCGA+TGG + chr1.4:6270075-6270094 MS.gene57259:intron 35.0%
!! TTTTTGAAGGACAGATGTTG+TGG - chr1.4:6270213-6270232 None:intergenic 35.0%
!!! AAGGAGGATTTTTTACCGTA+AGG + chr1.4:6268395-6268414 MS.gene57259:CDS 35.0%
!!! ATTTGGATGGTTTTGGTTTC+TGG + chr1.4:6268283-6268302 MS.gene57259:intron 35.0%
!!! CAACTAGGGAGTGTTTTAAA+GGG - chr1.4:6269272-6269291 None:intergenic 35.0%
!!! TTTGGATGGTTTTGGTTTCT+GGG + chr1.4:6268284-6268303 MS.gene57259:intron 35.0%
!!! TTTTTAGTTGTGGGTGTTCT+TGG + chr1.4:6268355-6268374 MS.gene57259:CDS 35.0%
ATACATGCGTAGTGTCTTCT+CGG - chr1.4:6269035-6269054 None:intergenic 40.0%
ATGTTTCCTTGGAAGAGCTT+TGG + chr1.4:6270788-6270807 MS.gene57259:intron 40.0%
ATTGTGGAGAACAACGTTGA+AGG + chr1.4:6270734-6270753 MS.gene57259:intron 40.0%
CACAAGACTCTATCGTTATC+AGG - chr1.4:6268584-6268603 None:intergenic 40.0%
CTTGAGTGAGTATAGTCAGA+GGG - chr1.4:6269798-6269817 None:intergenic 40.0%
TAATGGGAATGCTAACCAAC+CGG - chr1.4:6268617-6268636 None:intergenic 40.0%
TAGTGTGATGCTCATCTACA+GGG + chr1.4:6270032-6270051 MS.gene57259:CDS 40.0%
TCTTGAGTGAGTATAGTCAG+AGG - chr1.4:6269799-6269818 None:intergenic 40.0%
TTAGTGTGATGCTCATCTAC+AGG + chr1.4:6270031-6270050 MS.gene57259:CDS 40.0%
TTATTGAGAGAATTGGCCAG+CGG + chr1.4:6269506-6269525 MS.gene57259:CDS 40.0%
TTTGGGAGAGAAGAGAAAGA+AGG - chr1.4:6268158-6268177 None:intergenic 40.0%
! AAGCTCATTGGTTGCACTAA+TGG + chr1.4:6269443-6269462 MS.gene57259:intron 40.0%
! ACAGGGAGCGTAAGTTTAAA+AGG + chr1.4:6270049-6270068 MS.gene57259:CDS 40.0%
! CTGATAACGATAGAGTCTTG+TGG + chr1.4:6268582-6268601 MS.gene57259:CDS 40.0%
! GGAATTATAGGGGTAGCTTT+TGG + chr1.4:6268536-6268555 MS.gene57259:CDS 40.0%
! GGACTCTCATATCTTGTTGT+TGG + chr1.4:6270809-6270828 MS.gene57259:intron 40.0%
! TTTGGAACACTTGTGCTTAG+CGG + chr1.4:6269993-6270012 MS.gene57259:CDS 40.0%
!! CGCCAAATTCTAGTTTGTCT+AGG + chr1.4:6270274-6270293 MS.gene57259:intron 40.0%
!! CTCATATCTTGTTGTTGGCT+TGG + chr1.4:6270814-6270833 MS.gene57259:intron 40.0%
!! TTGGTTTCTGGGTTTTTCAC+AGG + chr1.4:6268295-6268314 MS.gene57259:intron 40.0%
!!! AGCTGTTTTGAAAAGCTGTC+TGG + chr1.4:6268901-6268920 MS.gene57259:intron 40.0%
!!! GCAACTAGGGAGTGTTTTAA+AGG - chr1.4:6269273-6269292 None:intergenic 40.0%
AAGTAAAGGCTGCGAAAAGG+AGG - chr1.4:6269555-6269574 None:intergenic 45.0%
AGTAAAGGCTGCGAAAAGGA+GGG - chr1.4:6269554-6269573 None:intergenic 45.0%
CACAAGTAAAGGCTGCGAAA+AGG - chr1.4:6269558-6269577 None:intergenic 45.0%
CTCAAGCAGAGATATGGTCT+TGG + chr1.4:6268498-6268517 MS.gene57259:CDS 45.0%
GATAAACCCCTGTGCCAAAA+AGG - chr1.4:6269900-6269919 None:intergenic 45.0%
GTGATCACATTCACCGTGTT+GGG - chr1.4:6268325-6268344 None:intergenic 45.0%
GTTGCACTAATGGCAGATGA+TGG + chr1.4:6269453-6269472 MS.gene57259:intron 45.0%
TGATCACATTCACCGTGTTG+GGG - chr1.4:6268324-6268343 None:intergenic 45.0%
TGATGCGAGCAGTGAGTTTA+TGG + chr1.4:6269129-6269148 MS.gene57259:intron 45.0%
TGTGATCACATTCACCGTGT+TGG - chr1.4:6268326-6268345 None:intergenic 45.0%
!! AGTAGGAGAGCGTGGAATTT+TGG - chr1.4:6268176-6268195 None:intergenic 45.0%
!! GTAGGAGAGCGTGGAATTTT+GGG - chr1.4:6268175-6268194 None:intergenic 45.0%
!! TGTTGGCTTGGTGACAGATT+AGG + chr1.4:6270826-6270845 MS.gene57259:intron 45.0%
GAGAGTCCAAAGCTCTTCCA+AGG - chr1.4:6270797-6270816 None:intergenic 50.0%
GATTGCGCAAGTCAGCGAAT+TGG - chr1.4:6268204-6268223 None:intergenic 50.0%
GCAGCTGCTGCAATAGCTAA+AGG - chr1.4:6269765-6269784 None:intergenic 50.0%
TACAACACATCCCAATCCGC+TGG - chr1.4:6269525-6269544 None:intergenic 50.0%
TCTAGGCTTTCGAGACCATC+AGG + chr1.4:6270291-6270310 MS.gene57259:intron 50.0%
TTTCACAGGTGTCCCCAACA+CGG + chr1.4:6268309-6268328 MS.gene57259:intron 50.0%
TTTGACCGCAGCATGTGAGA+TGG - chr1.4:6270337-6270356 None:intergenic 50.0%
! AGAGAATTGGCCAGCGGATT+GGG + chr1.4:6269512-6269531 MS.gene57259:CDS 50.0%
! AGTCTTGTGGGACACTTTGC+CGG + chr1.4:6268595-6268614 MS.gene57259:CDS 50.0%
! GCACTAATGGCAGATGATGG+TGG + chr1.4:6269456-6269475 MS.gene57259:intron 50.0%
! GGACAGATGTTGTGGTCTTG+TGG - chr1.4:6270205-6270224 None:intergenic 50.0%
!! AAGCCTGACCTTTTTGGCAC+AGG + chr1.4:6269889-6269908 MS.gene57259:intron 50.0%
!! AGCCTGACCTTTTTGGCACA+GGG + chr1.4:6269890-6269909 MS.gene57259:intron 50.0%
!! GCTGACTTGCGCAATCTTCT+TGG + chr1.4:6268208-6268227 MS.gene57259:CDS 50.0%
!! TCAGCGAATTGGTGGTGAGT+AGG - chr1.4:6268193-6268212 None:intergenic 50.0%
ACCCCTGTGCCAAAAAGGTC+AGG - chr1.4:6269895-6269914 None:intergenic 55.0%
CGTTATCAGCGGGCACTCTT+TGG + chr1.4:6269975-6269994 MS.gene57259:CDS 55.0%
GAGAGAATTGGCCAGCGGAT+TGG + chr1.4:6269511-6269530 MS.gene57259:CDS 55.0%
GAGCTCCATCTCACATGCTG+CGG + chr1.4:6270329-6270348 MS.gene57259:intron 55.0%
TGCGCAAGTCAGCGAATTGG+TGG - chr1.4:6268201-6268220 None:intergenic 55.0%
TTGTGGGACACTTTGCCGGT+TGG + chr1.4:6268599-6268618 MS.gene57259:intron 55.0%
!! GCCTGACCTTTTTGGCACAG+GGG + chr1.4:6269891-6269910 MS.gene57259:intron 55.0%
! TGGTGGTGAGTAGGAGAGCG+TGG - chr1.4:6268184-6268203 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 6268031 6270870 6268031 ID=MS.gene57259
chr1.4 mRNA 6268031 6270870 6268031 ID=MS.gene57259.t1;Parent=MS.gene57259
chr1.4 exon 6268031 6268229 6268031 ID=MS.gene57259.t1.exon1;Parent=MS.gene57259.t1
chr1.4 CDS 6268031 6268229 6268031 ID=cds.MS.gene57259.t1;Parent=MS.gene57259.t1
chr1.4 exon 6268317 6268416 6268317 ID=MS.gene57259.t1.exon2;Parent=MS.gene57259.t1
chr1.4 CDS 6268317 6268416 6268317 ID=cds.MS.gene57259.t1;Parent=MS.gene57259.t1
chr1.4 exon 6268496 6268616 6268496 ID=MS.gene57259.t1.exon3;Parent=MS.gene57259.t1
chr1.4 CDS 6268496 6268616 6268496 ID=cds.MS.gene57259.t1;Parent=MS.gene57259.t1
chr1.4 exon 6269469 6269590 6269469 ID=MS.gene57259.t1.exon4;Parent=MS.gene57259.t1
chr1.4 CDS 6269469 6269590 6269469 ID=cds.MS.gene57259.t1;Parent=MS.gene57259.t1
chr1.4 exon 6269716 6269837 6269716 ID=MS.gene57259.t1.exon5;Parent=MS.gene57259.t1
chr1.4 CDS 6269716 6269837 6269716 ID=cds.MS.gene57259.t1;Parent=MS.gene57259.t1
chr1.4 exon 6269911 6270070 6269911 ID=MS.gene57259.t1.exon6;Parent=MS.gene57259.t1
chr1.4 CDS 6269911 6270070 6269911 ID=cds.MS.gene57259.t1;Parent=MS.gene57259.t1
chr1.4 exon 6270843 6270870 6270843 ID=MS.gene57259.t1.exon7;Parent=MS.gene57259.t1
chr1.4 CDS 6270843 6270870 6270843 ID=cds.MS.gene57259.t1;Parent=MS.gene57259.t1
Gene Sequence

>MS.gene57259

ATGATGATTCAGAATTGGAGTTTGAATTGGATGGTAGCACATGCAAACAAACAACAACAACAATTGAGAAGATTGAAGTTAACAACTCATCGTGTTATTGTAGCTACATTTCTCTTCTCTATCTCCTTCTTTCTCTTCTCTCCCAAAATTCCACGCTCTCCTACTCACCACCAATTCGCTGACTTGCGCAATCTTCTTGGTAAAAAAATTCACTTTCTATTTAATTTGATGAAAAAGATTTAATTTTTCTTAATTTGGATGGTTTTGGTTTCTGGGTTTTTCACAGGTGTCCCCAACACGGTGAATGTGATCACAAATTTCCCATTTTTAGTTGTGGGTGTTCTTGGTTTAGTTCTTACTCTTGAAGGAGGATTTTTTACCGTAAGGTTAGTTACTTTTATCATCACTGTAATAGAATTTACTGTGATTAGATTCTAATATGAGGTGTGTTTTTGATTTGGTTAGTTCTCAAGCAGAGATATGGTCTTGGATACTATTCTATGCTGGAATTATAGGGGTAGCTTTTGGCTCTGTTTATTACCATTTGAAGCCTGATAACGATAGAGTCTTGTGGGACACTTTGCCGGTTGGTTAGCATTCCCATTACCTACCTCTCTATATGAATATATTTATATATGTTATTACTATTTGTATGATTCTTGAGTTGTTTTCCTTTTCCATGATTGTTTTGGCTAAAGAACTTAATTAAGCACTTACTCGATACACACTTGTCAAGTTATCATATAAGAGTTTATGTGTAAGCTATTTCAATAATGTAAGGTCTAGTTTGGATAAACAACTTAATTAAGCGCTTATCATATAAGTGCTTATGTATAAGTTATTTCTGTAACAAAAGATAAACCAAAGTCAAGCTGTTTTGAAAAGCTGTCTGGCAGAGCTTACGAAAATAAGCTAAAAATAGCTTATGGACATGTCATAAGCTGTTTTATAAGCTCTCTCAAAACTCTCACAAATACGTAGATAAGCTCAAATAAGTCAATCCGAGAAGACACTACGCATGTATAAACTATTTCTATATTTGAATAGGGGATGCGGTAAAACTCCACATAAGCTCTCTTCTATAATTCTATTAAGTTATGATGCGAGCAGTGAGTTTATGGAAATAAGCTCAACACAACTTATAAACTTGTCATAAGCTCTTTTTAAAATTAATCTCTTCAAAAATTATTCCAAACTCACACAAGTGTTATTGATGTCATTAGATAAAGCCAAATAAGCCCTTTAAAACACTCCCTAGTTGCTCCATTTGTTTAAATTATGACAGAAGTGAGCACTTATAAAGAGAAAATGTTAAGGTGGAATGCATTTTATCTGTTAAAAACACTTCTTTTGCTAGCTGAAAATCTAGACTTACCTCTTAGTACTTAGTTCGTTTTTTCCCCTTTATTAAGAAGCTCATTGGTTGCACTAATGGCAGATGATGGTGGCATATTCCTCACTTTTCTCTAGTTTGGTTATTGAGAGAATTGGCCAGCGGATTGGGATGTGTTGTATGTGTGCCCTCCTTTTCGCAGCCTTTACTTGTGTTGTTTATGAACGGTTAGTTCTTATCTTCCTCTAATAATAGTCTCAAGCTTATTCTCTCTTTTAAATGTTTTATACTTTGAGGTGCTGAACTTGCCACTAAGTTTATGCTAAAATTATGATCTGCTAGTTGGTTGCAGAACTTATGATGATATTCGGCTCTGCTTGATGTTCCAGTTCATTCTTCCTTTAGCTATTGCAGCAGCTGCATTTGTATATCCCTCTGACTATACTCACTCAAGATATTGGTTTTCATCAATAGGTATTATGCCTCAGACTTTTGCAACATTTATTCATAAATTTCAAATATTTGAAGCCTGACCTTTTTGGCACAGGGGTTTATCTGCTAGCAAAATTTGAAGCTGTTTCTGACAAGAAACTCTACCGTGCAAATAACTACGTTATCAGCGGGCACTCTTTGGAACACTTGTGCTTAGCGGTTATCCCGTTTTTACTTAGTGTGATGCTCATCTACAGGGAGCGTAAGTTTAAAAGGTATTTGATGATGAACCTATTCGATGGAATTTTTGGTTCCTTGTGAAATTTGGTTTCACAAATTGTTCTGGTTATTCTGTTTCTACAAATTTTTGAGTGAATCCTCAAGTCAGTTTTCCAAATTCAAACATCCACAAGACCACAACATCTGTCCTTCAAAAATTTGAAATGTCATCCCACTAGTTTTTTTCTTCAGAAGTAGTCGCCAAATTCTAGTTTGTCTAGGCTTTCGAGACCATCAGGAATCGATTTTGATCAGAGCTCCATCTCACATGCTGCGGTCAAAATCGATTTTTACCTCACCATGAATTGGTTTTTGATTAGGCTATTAGCACGTGTTTGTTTTGGTGATTGACAAAAAGTGAACGTGCATTGCTTTGTTAGCTACAATTTGTAGCTTGTGCATTTCCCCTGATTGACTTATTTTAGTTTATATACTGATATAAACATTTTTGAGACTGTTTGTGATAGCTAATGAAAACAGCTTATCATAATCTCTTTGTGATAAGCTGTTTTAAGCTTATTTTCATAAGCCCTCCAAGATATCTTATGAAAACAATTTATAGCTTATATGAAAACAACTTGACTTTCTTTTATCTTTTGTTATATAAATAACTTATATATAAACAATTATATGATAAATGCTTATGCTATAAGCACTTAATTGTGGAGAACAACGTTGAAGGTTTACTATTCTCTTTTTCCTTTTTGTAATTAATGTTTCCTTGGAAGAGCTTTGGACTCTCATATCTTGTTGTTGGCTTGGTGACAGATTAGGAGATGTTAAAGATCAGCTTTGA
Protein sequence

>MS.gene57259.t1

MMIQNWSLNWMVAHANKQQQQLRRLKLTTHRVIVATFLFSISFFLFSPKIPRSPTHHQFADLRNLLGVPNTVNVITNFPFLVVGVLGLVLTLEGGFFTVSSQAEIWSWILFYAGIIGVAFGSVYYHLKPDNDRVLWDTLPMMVAYSSLFSSLVIERIGQRIGMCCMCALLFAAFTCVVYERTYDDIRLCLMFQFILPLAIAAAAFVYPSDYTHSRYWFSSIGVYLLAKFEAVSDKKLYRANNYVISGHSLEHLCLAVIPFLLSVMLIYRERKFKRLGDVKDQL