AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57490.t1 MTR_0002s0040 53.698 311 103 10 1 290 1 291 2.50e-95 283
MS.gene57490.t1 MTR_0263s0020 56.164 292 96 9 1 280 1 272 4.21e-94 280
MS.gene57490.t1 MTR_1457s0010 66.234 231 54 8 65 291 1 211 2.88e-93 275
MS.gene57490.t1 MTR_0002s1060 51.546 291 108 11 4 280 6 277 3.70e-75 232
MS.gene57490.t1 MTR_0062s0020 38.542 288 140 8 14 280 4 275 1.39e-48 164
MS.gene57490.t1 MTR_0062s0090 39.510 286 138 10 14 280 4 273 8.98e-48 161
MS.gene57490.t1 MTR_0062s0040 39.510 286 138 10 14 280 4 273 8.98e-48 161
MS.gene57490.t1 MTR_0062s0060 39.510 286 138 10 14 280 4 273 8.98e-48 161
MS.gene57490.t1 MTR_0443s0040 39.175 291 132 12 14 280 16 285 1.26e-47 161
MS.gene57490.t1 MTR_0062s0170 36.304 303 151 10 1 280 1 284 8.30e-39 139
MS.gene57490.t1 MTR_0062s0110 36.304 303 151 10 1 280 1 284 8.30e-39 139
MS.gene57490.t1 MTR_0062s0130 36.304 303 151 10 1 280 1 284 8.30e-39 139
MS.gene57490.t1 MTR_2g036240 36.304 303 151 10 1 280 1 284 8.30e-39 139
MS.gene57490.t1 MTR_2g036260 36.304 303 151 10 1 280 1 284 8.30e-39 139
MS.gene57490.t1 MTR_2g036290 36.304 303 151 10 1 280 1 284 8.30e-39 139
MS.gene57490.t1 MTR_8g046730 36.304 303 151 10 1 280 1 284 8.30e-39 139
MS.gene57490.t1 MTR_6g079630 34.182 275 156 8 12 280 32 287 3.22e-37 134
MS.gene57490.t1 MTR_2g096030 32.990 291 163 10 14 291 31 302 1.39e-33 124
MS.gene57490.t1 MTR_2g096040 33.202 253 145 6 39 283 26 262 2.08e-28 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGGAATATAAAAGATTT+TGG 0.113401 8.3:-61447029 None:intergenic
AATCGATTTCCATGTCATTT+TGG 0.208877 8.3:+61447067 MS.gene57490:CDS
ACCACAGTGACGATATTAAT+TGG 0.223661 8.3:+61447540 MS.gene57490:CDS
ATTTCGTTGTAGTCAATATC+AGG 0.268052 8.3:-61447047 None:intergenic
GGAGAGAAAGGATATTTAGT+AGG 0.309124 8.3:-61447284 None:intergenic
ATCCAAGACTACACGTTTGA+TGG 0.357506 8.3:+61447359 MS.gene57490:CDS
TGTAGTGTTCATTAGTTTCA+TGG 0.361129 8.3:-61447502 None:intergenic
GAGAGAAAGGATATTTAGTA+GGG 0.368934 8.3:-61447283 None:intergenic
GTTGGAGAAATCTTCTTCAT+TGG 0.372094 8.3:-61447411 None:intergenic
GAATCGATCAACAGTGTTAC+CGG 0.376698 8.3:-61446973 None:intergenic
AAGATTTCTCCAACTACGTT+GGG 0.393833 8.3:+61447420 MS.gene57490:CDS
CATCTTCGTTGTAAGTCTCA+TGG 0.421031 8.3:-61447097 None:intergenic
GTAGTGTTCATTAGTTTCAT+GGG 0.454037 8.3:-61447501 None:intergenic
GGTTAGGCTGCATGTAGAAT+TGG 0.458886 8.3:-61447571 None:intergenic
AACACTACAAGCTACTCTAC+AGG 0.460172 8.3:+61447516 MS.gene57490:CDS
CGGAAAATTCTATCCCGATT+GGG 0.468618 8.3:+61447130 MS.gene57490:CDS
CCACAGTGACGATATTAATT+GGG 0.471394 8.3:+61447541 MS.gene57490:CDS
TGCCATCAAACGTGTAGTCT+TGG 0.476476 8.3:-61447361 None:intergenic
CTAAGTACCACCTTGACCTT+GGG 0.479326 8.3:-61447203 None:intergenic
GATCGGTGCTTGCTCCTACT+AGG 0.491839 8.3:-61447667 None:intergenic
GCAGATATCAAGATCCCAAT+CGG 0.494886 8.3:-61447144 None:intergenic
TGTTCATTAGTTTCATGGGA+TGG 0.496934 8.3:-61447497 None:intergenic
TATAACGTCCCCAACGTAGT+TGG 0.497459 8.3:-61447429 None:intergenic
TTGCGAGTCTAACATAGATC+AGG 0.504007 8.3:-61447619 None:intergenic
CTGCGGTATACCACGTGTGC+AGG 0.504725 8.3:+61447163 MS.gene57490:CDS
CCGGAAAATTCTATCCCGAT+TGG 0.509755 8.3:+61447129 MS.gene57490:CDS
GCTAAGTACCACCTTGACCT+TGG 0.518197 8.3:-61447204 None:intergenic
TCAGTCTCGTCAGTGGGGTT+AGG 0.532019 8.3:-61447587 None:intergenic
ACGAAGACATCTAGTGGAAG+TGG 0.532647 8.3:-61447710 None:intergenic
CATGAGACTTACAACGAAGA+TGG 0.533162 8.3:+61447098 MS.gene57490:CDS
ATCTTCGTTGTAAGTCTCAT+GGG 0.537135 8.3:-61447096 None:intergenic
GAGAAAGGATATTTAGTAGG+GGG 0.545897 8.3:-61447281 None:intergenic
ACTTACAACGAAGATGGAAA+AGG 0.547716 8.3:+61447104 MS.gene57490:CDS
TACAAAGTCAGTCTCGTCAG+TGG 0.547959 8.3:-61447594 None:intergenic
ACAAAGTCAGTCTCGTCAGT+GGG 0.548454 8.3:-61447593 None:intergenic
GTCAAGGTGGTACTTAGCAT+TGG 0.558729 8.3:+61447209 MS.gene57490:CDS
TGATCTATGTTAGACTCGCA+AGG 0.562801 8.3:+61447621 MS.gene57490:CDS
GAAGATTTCTCCAACTACGT+TGG 0.566238 8.3:+61447419 MS.gene57490:CDS
ATTACGTCCGTCACGTGGAT+CGG 0.567052 8.3:-61447684 None:intergenic
TCGTGATATACAAAGTGTGC+AGG 0.567577 8.3:+61447235 MS.gene57490:CDS
AAGGTGGTACTTAGCATTGG+TGG 0.572579 8.3:+61447212 MS.gene57490:CDS
AGTTTCATGGGATGGAGCAA+TGG 0.579271 8.3:-61447489 None:intergenic
TTTCGTTGTAGTCAATATCA+GGG 0.579571 8.3:-61447046 None:intergenic
AACGAAGATGGAAAAGGCAC+CGG 0.582283 8.3:+61447110 MS.gene57490:CDS
CAGATATCAAGATCCCAATC+GGG 0.583723 8.3:-61447143 None:intergenic
CTCGCAACGAAGACATCTAG+TGG 0.584791 8.3:-61447716 None:intergenic
AGAGAAAGGATATTTAGTAG+GGG 0.590103 8.3:-61447282 None:intergenic
GGGCAAAGGCCAAAATGACA+TGG 0.593006 8.3:-61447076 None:intergenic
GCGTTGAACCTTCATGAGAT+CGG 0.594521 8.3:-61447744 None:intergenic
AAAAGACTCAAGGAAGAGGT+AGG 0.596213 8.3:+61447452 MS.gene57490:CDS
GGAACATTACGTCCGTCACG+TGG 0.606987 8.3:-61447689 None:intergenic
ACAATGTCTCATCTCATCAC+CGG 0.607947 8.3:+61446954 None:intergenic
GAGTTGCTCCGATCTCATGA+AGG 0.616774 8.3:+61447736 MS.gene57490:CDS
CTCATGAAGGTTCAACGCAG+TGG 0.621602 8.3:+61447749 MS.gene57490:CDS
GTTGTAAGTCTCATGGGCAA+AGG 0.623453 8.3:-61447090 None:intergenic
GATCTATGTTAGACTCGCAA+GGG 0.624736 8.3:+61447622 MS.gene57490:CDS
GCTCAAGTATCCCAAGGTCA+AGG 0.636705 8.3:+61447193 MS.gene57490:CDS
ACACTACAAGCTACTCTACA+GGG 0.639330 8.3:+61447517 MS.gene57490:CDS
AGCTTGATATCCTGCACACG+TGG 0.641314 8.3:-61447173 None:intergenic
ATCGACGGCATTATCACACC+AGG 0.643275 8.3:-61447321 None:intergenic
GCAAGCACCGATCCACGTGA+CGG 0.658997 8.3:+61447677 MS.gene57490:CDS
AGATTTCTCCAACTACGTTG+GGG 0.668094 8.3:+61447421 MS.gene57490:CDS
TATCAAGCTCAAGTATCCCA+AGG 0.668476 8.3:+61447187 MS.gene57490:CDS
CCCAATTAATATCGTCACTG+TGG 0.680315 8.3:-61447541 None:intergenic
GCAGGATATCATCAAGCACA+AGG 0.687092 8.3:+61447253 MS.gene57490:CDS
CAAGTATCCCAAGGTCAAGG+TGG 0.692556 8.3:+61447196 MS.gene57490:CDS
CAAAGTCAGTCTCGTCAGTG+GGG 0.694670 8.3:-61447592 None:intergenic
GATTGGGATCTTGATATCTG+CGG 0.706223 8.3:+61447146 MS.gene57490:CDS
GGAAGAGGTAGGTATCAATG+TGG 0.752690 8.3:+61447463 MS.gene57490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATAAAAGATTTTGGGTA+TGG - chr8.3:61447026-61447045 None:intergenic 20.0%
!! CAGGGAATATAAAAGATTTT+GGG - chr8.3:61447031-61447050 None:intergenic 25.0%
!! TCAGGGAATATAAAAGATTT+TGG - chr8.3:61447032-61447051 None:intergenic 25.0%
!! TTCATTTTACGAGAATACAT+TGG + chr8.3:61446993-61447012 MS.gene57490:CDS 25.0%
AGAGAAAGGATATTTAGTAG+GGG - chr8.3:61447285-61447304 None:intergenic 30.0%
ATTTCGTTGTAGTCAATATC+AGG - chr8.3:61447050-61447069 None:intergenic 30.0%
GAGAGAAAGGATATTTAGTA+GGG - chr8.3:61447286-61447305 None:intergenic 30.0%
TATAAAAAGACTCAAGGAAG+AGG + chr8.3:61447448-61447467 MS.gene57490:CDS 30.0%
TTTCGTTGTAGTCAATATCA+GGG - chr8.3:61447049-61447068 None:intergenic 30.0%
! GTAGTGTTCATTAGTTTCAT+GGG - chr8.3:61447504-61447523 None:intergenic 30.0%
! TGTAGTGTTCATTAGTTTCA+TGG - chr8.3:61447505-61447524 None:intergenic 30.0%
!! AATCGATTTCCATGTCATTT+TGG + chr8.3:61447067-61447086 MS.gene57490:CDS 30.0%
!! ACTTTTGAAAAACTCCTAGT+AGG + chr8.3:61447653-61447672 MS.gene57490:CDS 30.0%
!!! TCTTTTTCAATAGGAGAGAA+AGG - chr8.3:61447299-61447318 None:intergenic 30.0%
AAGATTTCTCCAACTACGTT+GGG + chr8.3:61447420-61447439 MS.gene57490:CDS 35.0%
ACCACAGTGACGATATTAAT+TGG + chr8.3:61447540-61447559 MS.gene57490:CDS 35.0%
ACTTACAACGAAGATGGAAA+AGG + chr8.3:61447104-61447123 MS.gene57490:CDS 35.0%
ATCTTCGTTGTAAGTCTCAT+GGG - chr8.3:61447099-61447118 None:intergenic 35.0%
CCACAGTGACGATATTAATT+GGG + chr8.3:61447541-61447560 MS.gene57490:CDS 35.0%
CTCCTATTGAAAAAGAATCC+TGG + chr8.3:61447303-61447322 MS.gene57490:CDS 35.0%
GAGAAAGGATATTTAGTAGG+GGG - chr8.3:61447284-61447303 None:intergenic 35.0%
GGACGTTATAAAAAGACTCA+AGG + chr8.3:61447442-61447461 MS.gene57490:CDS 35.0%
GGAGAGAAAGGATATTTAGT+AGG - chr8.3:61447287-61447306 None:intergenic 35.0%
GTTGGAGAAATCTTCTTCAT+TGG - chr8.3:61447414-61447433 None:intergenic 35.0%
! TGTTCATTAGTTTCATGGGA+TGG - chr8.3:61447500-61447519 None:intergenic 35.0%
!!! CACCAGGATTCTTTTTCAAT+AGG - chr8.3:61447308-61447327 None:intergenic 35.0%
AAAAGACTCAAGGAAGAGGT+AGG + chr8.3:61447452-61447471 MS.gene57490:CDS 40.0%
AACACTACAAGCTACTCTAC+AGG + chr8.3:61447516-61447535 MS.gene57490:CDS 40.0%
ACACTACAAGCTACTCTACA+GGG + chr8.3:61447517-61447536 MS.gene57490:CDS 40.0%
AGATTTCTCCAACTACGTTG+GGG + chr8.3:61447421-61447440 MS.gene57490:CDS 40.0%
ATCCAAGACTACACGTTTGA+TGG + chr8.3:61447359-61447378 MS.gene57490:CDS 40.0%
CAGATATCAAGATCCCAATC+GGG - chr8.3:61447146-61447165 None:intergenic 40.0%
CATCTTCGTTGTAAGTCTCA+TGG - chr8.3:61447100-61447119 None:intergenic 40.0%
CATGAGACTTACAACGAAGA+TGG + chr8.3:61447098-61447117 MS.gene57490:CDS 40.0%
CCCAATTAATATCGTCACTG+TGG - chr8.3:61447544-61447563 None:intergenic 40.0%
CGGAAAATTCTATCCCGATT+GGG + chr8.3:61447130-61447149 MS.gene57490:CDS 40.0%
GAAGATTTCTCCAACTACGT+TGG + chr8.3:61447419-61447438 MS.gene57490:CDS 40.0%
GAATCGATCAACAGTGTTAC+CGG - chr8.3:61446976-61446995 None:intergenic 40.0%
GATCTATGTTAGACTCGCAA+GGG + chr8.3:61447622-61447641 MS.gene57490:CDS 40.0%
GATTGGGATCTTGATATCTG+CGG + chr8.3:61447146-61447165 MS.gene57490:CDS 40.0%
GCAGATATCAAGATCCCAAT+CGG - chr8.3:61447147-61447166 None:intergenic 40.0%
TATCAAGCTCAAGTATCCCA+AGG + chr8.3:61447187-61447206 MS.gene57490:CDS 40.0%
TCGTGATATACAAAGTGTGC+AGG + chr8.3:61447235-61447254 MS.gene57490:CDS 40.0%
TGATCTATGTTAGACTCGCA+AGG + chr8.3:61447621-61447640 MS.gene57490:CDS 40.0%
TTGCGAGTCTAACATAGATC+AGG - chr8.3:61447622-61447641 None:intergenic 40.0%
! AACGCAGTGGCATTTTCATT+TGG + chr8.3:61447762-61447781 MS.gene57490:CDS 40.0%
! CCAATCGGGATAGAATTTTC+CGG - chr8.3:61447132-61447151 None:intergenic 40.0%
!! GATCTTTTTGAGCGAATCGA+CGG - chr8.3:61447339-61447358 None:intergenic 40.0%
ACAAAGTCAGTCTCGTCAGT+GGG - chr8.3:61447596-61447615 None:intergenic 45.0%
ACGAAGACATCTAGTGGAAG+TGG - chr8.3:61447713-61447732 None:intergenic 45.0%
AGTTTCATGGGATGGAGCAA+TGG - chr8.3:61447492-61447511 None:intergenic 45.0%
CCGGAAAATTCTATCCCGAT+TGG + chr8.3:61447129-61447148 MS.gene57490:CDS 45.0%
CTAAGTACCACCTTGACCTT+GGG - chr8.3:61447206-61447225 None:intergenic 45.0%
GCAGGATATCATCAAGCACA+AGG + chr8.3:61447253-61447272 MS.gene57490:CDS 45.0%
GCGTTGAACCTTCATGAGAT+CGG - chr8.3:61447747-61447766 None:intergenic 45.0%
GGAAGAGGTAGGTATCAATG+TGG + chr8.3:61447463-61447482 MS.gene57490:CDS 45.0%
GGTTAGGCTGCATGTAGAAT+TGG - chr8.3:61447574-61447593 None:intergenic 45.0%
GTCAAGGTGGTACTTAGCAT+TGG + chr8.3:61447209-61447228 MS.gene57490:CDS 45.0%
GTTGTAAGTCTCATGGGCAA+AGG - chr8.3:61447093-61447112 None:intergenic 45.0%
TACAAAGTCAGTCTCGTCAG+TGG - chr8.3:61447597-61447616 None:intergenic 45.0%
TATAACGTCCCCAACGTAGT+TGG - chr8.3:61447432-61447451 None:intergenic 45.0%
TGCCATCAAACGTGTAGTCT+TGG - chr8.3:61447364-61447383 None:intergenic 45.0%
! AACGAAGATGGAAAAGGCAC+CGG + chr8.3:61447110-61447129 MS.gene57490:CDS 45.0%
! AAGGTGGTACTTAGCATTGG+TGG + chr8.3:61447212-61447231 MS.gene57490:CDS 45.0%
AGCTTGATATCCTGCACACG+TGG - chr8.3:61447176-61447195 None:intergenic 50.0%
ATCGACGGCATTATCACACC+AGG - chr8.3:61447324-61447343 None:intergenic 50.0%
ATTACGTCCGTCACGTGGAT+CGG - chr8.3:61447687-61447706 None:intergenic 50.0%
CAAAGTCAGTCTCGTCAGTG+GGG - chr8.3:61447595-61447614 None:intergenic 50.0%
CAAGTATCCCAAGGTCAAGG+TGG + chr8.3:61447196-61447215 MS.gene57490:CDS 50.0%
CTCATGAAGGTTCAACGCAG+TGG + chr8.3:61447749-61447768 MS.gene57490:CDS 50.0%
CTCGCAACGAAGACATCTAG+TGG - chr8.3:61447719-61447738 None:intergenic 50.0%
GAGTTGCTCCGATCTCATGA+AGG + chr8.3:61447736-61447755 MS.gene57490:CDS 50.0%
GCTAAGTACCACCTTGACCT+TGG - chr8.3:61447207-61447226 None:intergenic 50.0%
GCTCAAGTATCCCAAGGTCA+AGG + chr8.3:61447193-61447212 MS.gene57490:CDS 50.0%
GGGCAAAGGCCAAAATGACA+TGG - chr8.3:61447079-61447098 None:intergenic 50.0%
GGAACATTACGTCCGTCACG+TGG - chr8.3:61447692-61447711 None:intergenic 55.0%
TCAGTCTCGTCAGTGGGGTT+AGG - chr8.3:61447590-61447609 None:intergenic 55.0%
! GATCGGTGCTTGCTCCTACT+AGG - chr8.3:61447670-61447689 None:intergenic 55.0%
CTGCGGTATACCACGTGTGC+AGG + chr8.3:61447163-61447182 MS.gene57490:CDS 60.0%
!! GCAAGCACCGATCCACGTGA+CGG + chr8.3:61447677-61447696 MS.gene57490:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 61446957 61447836 61446957 ID=MS.gene57490
chr8.3 mRNA 61446957 61447836 61446957 ID=MS.gene57490.t1;Parent=MS.gene57490
chr8.3 exon 61446957 61447836 61446957 ID=MS.gene57490.t1.exon1;Parent=MS.gene57490.t1
chr8.3 CDS 61446957 61447836 61446957 ID=cds.MS.gene57490.t1;Parent=MS.gene57490.t1
Gene Sequence

>MS.gene57490

ATGTCTCATCTCATCACCGGTAACACTGTTGATCGATTCATTTTACGAGAATACATTGGCGTGAAACCATACCCAAAATCTTTTATATTCCCTGATATTGACTACAACGAAATCGATTTCCATGTCATTTTGGCCTTTGCCCATGAGACTTACAACGAAGATGGAAAAGGCACCGGAAAATTCTATCCCGATTGGGATCTTGATATCTGCGGTATACCACGTGTGCAGGATATCAAGCTCAAGTATCCCAAGGTCAAGGTGGTACTTAGCATTGGTGGTCGTGATATACAAAGTGTGCAGGATATCATCAAGCACAAGGATATTCCCCCTACTAAATATCCTTTCTCTCCTATTGAAAAAGAATCCTGGTGTGATAATGCCGTCGATTCGCTCAAAAAGATCATCCAAGACTACACGTTTGATGGCATTGATATTCTCTATGACAACATCAATACCAATGAAGAAGATTTCTCCAACTACGTTGGGGACGTTATAAAAAGACTCAAGGAAGAGGTAGGTATCAATGTGGTGTCCATTGCTCCATCCCATGAAACTAATGAACACTACAAGCTACTCTACAGGGACCACAGTGACGATATTAATTGGGTTAACTACCAATTCTACATGCAGCCTAACCCCACTGACGAGACTGACTTTGTAGACCTGATCTATGTTAGACTCGCAAGGGAATGTTACACTTTTGAAAAACTCCTAGTAGGAGCAAGCACCGATCCACGTGACGGACGTAATGTTCCACTTCCACTAGATGTCTTCGTTGCGAGTTGCTCCGATCTCATGAAGGTTCAACGCAGTGGCATTTTCATTTGGAATGCTAATGACTATGAGAAAATTGCACGAGATATCATAACAACTATCCCTC

Protein sequence

>MS.gene57490.t1

MSHLITGNTVDRFILREYIGVKPYPKSFIFPDIDYNEIDFHVILAFAHETYNEDGKGTGKFYPDWDLDICGIPRVQDIKLKYPKVKVVLSIGGRDIQSVQDIIKHKDIPPTKYPFSPIEKESWCDNAVDSLKKIIQDYTFDGIDILYDNINTNEEDFSNYVGDVIKRLKEEVGINVVSIAPSHETNEHYKLLYRDHSDDINWVNYQFYMQPNPTDETDFVDLIYVRLARECYTFEKLLVGASTDPRDGRNVPLPLDVFVASCSDLMKVQRSGIFIWNANDYEKIARDIITTIP