Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57543.t1 | XP_003627627.1 | 98 | 148 | 3 | 0 | 1 | 148 | 1 | 148 | 3.20E-76 | 294.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57543.t1 | Q9C8S6 | 58.1 | 148 | 61 | 1 | 1 | 148 | 1 | 147 | 5.8e-42 | 171.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57543.t1 | G7LCN6 | 98.0 | 148 | 3 | 0 | 1 | 148 | 1 | 148 | 2.3e-76 | 294.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055506 | MS.gene57543 | 0.805022 | 1.63E-49 | -1.69E-46 |
| MS.gene056677 | MS.gene57543 | 0.830106 | 3.54E-55 | -1.69E-46 |
| MS.gene059634 | MS.gene57543 | 0.809705 | 1.66E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57543.t1 | MTR_8g032320 | 97.973 | 148 | 3 | 0 | 1 | 148 | 1 | 148 | 3.61e-105 | 296 |
| MS.gene57543.t1 | MTR_8g032300 | 83.784 | 148 | 24 | 0 | 1 | 148 | 1 | 148 | 7.71e-93 | 265 |
| MS.gene57543.t1 | MTR_1g008950 | 64.000 | 125 | 45 | 0 | 1 | 125 | 1 | 125 | 1.13e-57 | 176 |
| MS.gene57543.t1 | MTR_5g079880 | 40.645 | 155 | 77 | 4 | 1 | 144 | 1 | 151 | 2.56e-37 | 125 |
| MS.gene57543.t1 | MTR_5g079890 | 40.506 | 158 | 78 | 5 | 1 | 145 | 1 | 155 | 9.01e-33 | 114 |
| MS.gene57543.t1 | MTR_5g079890 | 40.580 | 138 | 69 | 4 | 1 | 125 | 1 | 138 | 3.45e-29 | 104 |
| MS.gene57543.t1 | MTR_8g030870 | 33.108 | 148 | 85 | 4 | 1 | 135 | 1 | 147 | 6.34e-20 | 81.6 |
| MS.gene57543.t1 | MTR_7g075960 | 56.604 | 53 | 23 | 0 | 25 | 77 | 1 | 53 | 3.70e-19 | 76.3 |
| MS.gene57543.t1 | MTR_5g004800 | 26.984 | 126 | 90 | 2 | 6 | 130 | 15 | 139 | 1.07e-13 | 66.2 |
| MS.gene57543.t1 | MTR_2g027100 | 37.500 | 96 | 58 | 2 | 4 | 99 | 6 | 99 | 1.94e-12 | 61.6 |
| MS.gene57543.t1 | MTR_5g004870 | 27.731 | 119 | 84 | 2 | 6 | 123 | 11 | 128 | 2.83e-12 | 62.8 |
| MS.gene57543.t1 | MTR_3g106660 | 31.496 | 127 | 81 | 4 | 3 | 127 | 439 | 561 | 8.85e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57543.t1 | AT1G63220 | 58.108 | 148 | 61 | 1 | 1 | 148 | 1 | 147 | 8.90e-57 | 174 |
| MS.gene57543.t1 | AT1G63220 | 54.054 | 148 | 53 | 2 | 1 | 148 | 1 | 133 | 3.77e-48 | 152 |
| MS.gene57543.t1 | AT3G55470 | 36.076 | 158 | 87 | 4 | 1 | 147 | 1 | 155 | 6.72e-26 | 96.3 |
| MS.gene57543.t1 | AT3G55470 | 36.478 | 159 | 86 | 5 | 1 | 147 | 1 | 156 | 6.31e-25 | 94.0 |
| MS.gene57543.t1 | AT4G00467 | 32.331 | 133 | 79 | 3 | 1 | 122 | 1 | 133 | 1.47e-17 | 75.5 |
| MS.gene57543.t1 | AT4G00467 | 32.331 | 133 | 79 | 3 | 1 | 122 | 1 | 133 | 2.63e-17 | 75.1 |
| MS.gene57543.t1 | AT5G47710 | 37.755 | 98 | 59 | 2 | 2 | 99 | 4 | 99 | 2.26e-13 | 63.9 |
| MS.gene57543.t1 | AT5G47710 | 37.755 | 98 | 59 | 2 | 2 | 99 | 4 | 99 | 2.26e-13 | 63.9 |
| MS.gene57543.t1 | AT5G47710 | 37.755 | 98 | 59 | 2 | 2 | 99 | 4 | 99 | 2.26e-13 | 63.9 |
| MS.gene57543.t1 | AT5G47710 | 37.755 | 98 | 59 | 2 | 2 | 99 | 4 | 99 | 2.26e-13 | 63.9 |
| MS.gene57543.t1 | AT4G34150 | 27.966 | 118 | 83 | 2 | 6 | 122 | 12 | 128 | 8.34e-13 | 63.9 |
| MS.gene57543.t1 | AT5G04220 | 29.747 | 158 | 96 | 5 | 2 | 147 | 37 | 191 | 1.03e-12 | 64.3 |
| MS.gene57543.t1 | AT5G04220 | 29.747 | 158 | 96 | 5 | 2 | 147 | 259 | 413 | 4.82e-12 | 62.8 |
| MS.gene57543.t1 | AT5G04220 | 29.747 | 158 | 96 | 5 | 2 | 147 | 197 | 351 | 1.03e-11 | 61.6 |
Find 25 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATATTGTGGAGAGATTA+AGG | 0.258182 | 8.3:+62260128 | MS.gene57543:CDS |
| TATAATAATGATGATAATAT+TGG | 0.281672 | 8.3:+62259583 | MS.gene57543:CDS |
| TGAGCTGAATCTAAAGATAA+TGG | 0.346296 | 8.3:+62259549 | MS.gene57543:CDS |
| GCTCCTGTGCACTGTAACTA+AGG | 0.366774 | 8.3:-62259348 | None:intergenic |
| ACAAGATTGTAAGCATTCTC+TGG | 0.421167 | 8.3:-62260096 | None:intergenic |
| GAAAAGTCTCATTCCATTGT+GGG | 0.465952 | 8.3:-62259501 | None:intergenic |
| GAATCTAAAGATAATGGAAA+AGG | 0.493781 | 8.3:+62259555 | MS.gene57543:CDS |
| TCTTCAAACACTGCTTCTAA+AGG | 0.503608 | 8.3:-62260063 | None:intergenic |
| AAGAGGAGGAGACTGGTGGA+TGG | 0.524498 | 8.3:+62260299 | MS.gene57543:CDS |
| CACAAGAGTGCTGTTCAAGA+AGG | 0.553199 | 8.3:+62259370 | MS.gene57543:CDS |
| AGAAAAGTCTCATTCCATTG+TGG | 0.555092 | 8.3:-62259502 | None:intergenic |
| TACAATGAAGAGGAGGAGAC+TGG | 0.555239 | 8.3:+62260292 | MS.gene57543:CDS |
| GATTGTAAGCATTCTCTGGA+AGG | 0.568662 | 8.3:-62260092 | None:intergenic |
| CTGTAACTAAGGATCACATA+AGG | 0.576082 | 8.3:-62259337 | None:intergenic |
| TTAGAAGCAGTGTTTGAAGA+GGG | 0.587548 | 8.3:+62260066 | MS.gene57543:CDS |
| GATCCTTAGTTACAGTGCAC+AGG | 0.590583 | 8.3:+62259345 | MS.gene57543:CDS |
| GTGAAGGATGAAGAATATTG+TGG | 0.592520 | 8.3:+62260117 | MS.gene57543:CDS |
| TTTAGAAGCAGTGTTTGAAG+AGG | 0.610475 | 8.3:+62260065 | MS.gene57543:CDS |
| TGATCAAAGTTACAATGAAG+AGG | 0.613168 | 8.3:+62260282 | MS.gene57543:CDS |
| AATGAAGAGGAGGAGACTGG+TGG | 0.640663 | 8.3:+62260295 | MS.gene57543:CDS |
| GAATGCTTACAATCTTGTGA+AGG | 0.644186 | 8.3:+62260101 | MS.gene57543:CDS |
| TCAAAGTTACAATGAAGAGG+AGG | 0.645124 | 8.3:+62260285 | MS.gene57543:CDS |
| AATCAAAGAAAGATGCCTCG+TGG | 0.675663 | 8.3:+62258894 | None:intergenic |
| GTGCTGGATCTAACCCACAA+TGG | 0.679425 | 8.3:+62259488 | MS.gene57543:CDS |
| ATAACTTCAAGAGTTCCACG+AGG | 0.830018 | 8.3:-62258909 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATTTAACTAGTTAAAT+TGG | - | chr8.3:62259263-62259282 | None:intergenic | 10.0% |
| !! | TATAATAATGATGATAATAT+TGG | + | chr8.3:62259583-62259602 | MS.gene57543:CDS | 10.0% |
| !! | TAATATGTACTATCAATTAT+TGG | + | chr8.3:62260245-62260264 | MS.gene57543:intron | 15.0% |
| !!! | AATTGATAGTACATATTAAT+AGG | - | chr8.3:62260243-62260262 | None:intergenic | 15.0% |
| !!! | ATTGATAGTACATATTAATA+GGG | - | chr8.3:62260242-62260261 | None:intergenic | 15.0% |
| !!! | TAATAATCAAATCATTTTTC+AGG | - | chr8.3:62259118-62259137 | None:intergenic | 15.0% |
| !!! | TCTTTTTTATGAATAATATC+AGG | + | chr8.3:62259466-62259485 | MS.gene57543:intron | 15.0% |
| !! | AGAATGAAATATAAGCAAAA+AGG | + | chr8.3:62259726-62259745 | MS.gene57543:intron | 20.0% |
| !! | GAATGAAATATAAACAAAAG+TGG | - | chr8.3:62259803-62259822 | None:intergenic | 20.0% |
| !! | GTTTAAAAATTTACACCTAA+AGG | + | chr8.3:62259883-62259902 | MS.gene57543:intron | 20.0% |
| !! | TGTAAGTAATTCTATGTAAT+AGG | + | chr8.3:62258972-62258991 | MS.gene57543:intron | 20.0% |
| !!! | CTTTTGTTTATATTTCATTC+CGG | + | chr8.3:62259803-62259822 | MS.gene57543:intron | 20.0% |
| !!! | TTTTTGCTTATATTTCATTC+TGG | - | chr8.3:62259727-62259746 | None:intergenic | 20.0% |
| ! | AAAAATTTACACCTAAAGGA+GGG | + | chr8.3:62259887-62259906 | MS.gene57543:intron | 25.0% |
| ! | AAAATTGAAAGTCAAACTCT+TGG | - | chr8.3:62259073-62259092 | None:intergenic | 25.0% |
| ! | AGAAAAGTAAGTGTTAAAGT+TGG | + | chr8.3:62259826-62259845 | MS.gene57543:intron | 25.0% |
| ! | TAAAAATTTACACCTAAAGG+AGG | + | chr8.3:62259886-62259905 | MS.gene57543:intron | 25.0% |
| ! | TGAAATATAAGCAAAAAGGT+TGG | + | chr8.3:62259730-62259749 | MS.gene57543:intron | 25.0% |
| ! | TTTGGATAAAAGTTCATCAT+GGG | + | chr8.3:62259091-62259110 | MS.gene57543:intron | 25.0% |
| !! | TGTCTTTGATTCATCTTTTT+AGG | + | chr8.3:62259646-62259665 | MS.gene57543:intron | 25.0% |
| !! | TTTTGGATAAAAGTTCATCA+TGG | + | chr8.3:62259090-62259109 | MS.gene57543:intron | 25.0% |
| !!! | AGAGTTTGACTTTCAATTTT+TGG | + | chr8.3:62259073-62259092 | MS.gene57543:intron | 25.0% |
| !!! | GAATCTAAAGATAATGGAAA+AGG | + | chr8.3:62259555-62259574 | MS.gene57543:CDS | 25.0% |
| !!! | TTTGTTATTTTGTTCTCTAG+TGG | + | chr8.3:62259431-62259450 | MS.gene57543:intron | 25.0% |
| AGAAATTATCCATGCTAAGT+TGG | + | chr8.3:62259144-62259163 | MS.gene57543:intron | 30.0% | |
| AGAATATTGTGGAGAGATTA+AGG | + | chr8.3:62260128-62260147 | MS.gene57543:CDS | 30.0% | |
| GAAATTTCTGCATGAAACAA+TGG | + | chr8.3:62259045-62259064 | MS.gene57543:intron | 30.0% | |
| GTTTGACTATGTAACTTCAA+AGG | - | chr8.3:62259977-62259996 | None:intergenic | 30.0% | |
| TGAGCTGAATCTAAAGATAA+TGG | + | chr8.3:62259549-62259568 | MS.gene57543:CDS | 30.0% | |
| TGATCAAAGTTACAATGAAG+AGG | + | chr8.3:62260282-62260301 | MS.gene57543:CDS | 30.0% | |
| TTATGAATAATATCAGGTGC+TGG | + | chr8.3:62259472-62259491 | MS.gene57543:intron | 30.0% | |
| TTGCTTATATTTCATTCTGG+AGG | - | chr8.3:62259724-62259743 | None:intergenic | 30.0% | |
| !! | CTTTAACACTTACTTTTCTC+CGG | - | chr8.3:62259825-62259844 | None:intergenic | 30.0% |
| !! | TTATATAGCCTTATTACCTC+AGG | - | chr8.3:62260171-62260190 | None:intergenic | 30.0% |
| !!! | GTTATTTTGATCAGTGCTAA+AGG | + | chr8.3:62258927-62258946 | MS.gene57543:CDS | 30.0% |
| AAAATTGAACGAGATGTCAG+TGG | - | chr8.3:62260005-62260024 | None:intergenic | 35.0% | |
| ACAAGATTGTAAGCATTCTC+TGG | - | chr8.3:62260099-62260118 | None:intergenic | 35.0% | |
| AGAAAAGTCTCATTCCATTG+TGG | - | chr8.3:62259505-62259524 | None:intergenic | 35.0% | |
| CTGTAACTAAGGATCACATA+AGG | - | chr8.3:62259340-62259359 | None:intergenic | 35.0% | |
| GAAAAGTCTCATTCCATTGT+GGG | - | chr8.3:62259504-62259523 | None:intergenic | 35.0% | |
| GAATGCTTACAATCTTGTGA+AGG | + | chr8.3:62260101-62260120 | MS.gene57543:CDS | 35.0% | |
| GTGAAGGATGAAGAATATTG+TGG | + | chr8.3:62260117-62260136 | MS.gene57543:CDS | 35.0% | |
| GTTGGATTCTCATTGAAAGT+TGG | + | chr8.3:62259844-62259863 | MS.gene57543:intron | 35.0% | |
| TCAAAGTTACAATGAAGAGG+AGG | + | chr8.3:62260285-62260304 | MS.gene57543:CDS | 35.0% | |
| TCTTCAAACACTGCTTCTAA+AGG | - | chr8.3:62260066-62260085 | None:intergenic | 35.0% | |
| TTAGAAGCAGTGTTTGAAGA+GGG | + | chr8.3:62260066-62260085 | MS.gene57543:CDS | 35.0% | |
| TTTAGAAGCAGTGTTTGAAG+AGG | + | chr8.3:62260065-62260084 | MS.gene57543:CDS | 35.0% | |
| ! | CTTTTACACCTGAGGTAATA+AGG | + | chr8.3:62260160-62260179 | MS.gene57543:intron | 35.0% |
| ATAACTTCAAGAGTTCCACG+AGG | - | chr8.3:62258912-62258931 | None:intergenic | 40.0% | |
| GATTGTAAGCATTCTCTGGA+AGG | - | chr8.3:62260095-62260114 | None:intergenic | 40.0% | |
| TAGATCAGACCAACTTAGCA+TGG | - | chr8.3:62259156-62259175 | None:intergenic | 40.0% | |
| AGTTGCAAAGTCGACGTATG+TGG | - | chr8.3:62259779-62259798 | None:intergenic | 45.0% | |
| GATCCTTAGTTACAGTGCAC+AGG | + | chr8.3:62259345-62259364 | MS.gene57543:CDS | 45.0% | |
| TACAATGAAGAGGAGGAGAC+TGG | + | chr8.3:62260292-62260311 | MS.gene57543:CDS | 45.0% | |
| TTTCCTTTGTGCCCTCCTTT+AGG | - | chr8.3:62259901-62259920 | None:intergenic | 45.0% | |
| !! | CACAAGAGTGCTGTTCAAGA+AGG | + | chr8.3:62259370-62259389 | MS.gene57543:CDS | 45.0% |
| !! | TGCTCTCACTTTTACACCTG+AGG | + | chr8.3:62260152-62260171 | MS.gene57543:CDS | 45.0% |
| AATGAAGAGGAGGAGACTGG+TGG | + | chr8.3:62260295-62260314 | MS.gene57543:CDS | 50.0% | |
| GCTCCTGTGCACTGTAACTA+AGG | - | chr8.3:62259351-62259370 | None:intergenic | 50.0% | |
| GTGCTGGATCTAACCCACAA+TGG | + | chr8.3:62259488-62259507 | MS.gene57543:CDS | 50.0% | |
| ! | ACACCTAAAGGAGGGCACAA+AGG | + | chr8.3:62259895-62259914 | MS.gene57543:intron | 50.0% |
| AAGAGGAGGAGACTGGTGGA+TGG | + | chr8.3:62260299-62260318 | MS.gene57543:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 62258906 | 62260345 | 62258906 | ID=MS.gene57543 |
| chr8.3 | mRNA | 62258906 | 62260345 | 62258906 | ID=MS.gene57543.t1;Parent=MS.gene57543 |
| chr8.3 | exon | 62258906 | 62258972 | 62258906 | ID=MS.gene57543.t1.exon1;Parent=MS.gene57543.t1 |
| chr8.3 | CDS | 62258906 | 62258972 | 62258906 | ID=cds.MS.gene57543.t1;Parent=MS.gene57543.t1 |
| chr8.3 | exon | 62259326 | 62259391 | 62259326 | ID=MS.gene57543.t1.exon2;Parent=MS.gene57543.t1 |
| chr8.3 | CDS | 62259326 | 62259391 | 62259326 | ID=cds.MS.gene57543.t1;Parent=MS.gene57543.t1 |
| chr8.3 | exon | 62259488 | 62259614 | 62259488 | ID=MS.gene57543.t1.exon3;Parent=MS.gene57543.t1 |
| chr8.3 | CDS | 62259488 | 62259614 | 62259488 | ID=cds.MS.gene57543.t1;Parent=MS.gene57543.t1 |
| chr8.3 | exon | 62260059 | 62260173 | 62260059 | ID=MS.gene57543.t1.exon4;Parent=MS.gene57543.t1 |
| chr8.3 | CDS | 62260059 | 62260173 | 62260059 | ID=cds.MS.gene57543.t1;Parent=MS.gene57543.t1 |
| chr8.3 | exon | 62260274 | 62260345 | 62260274 | ID=MS.gene57543.t1.exon5;Parent=MS.gene57543.t1 |
| chr8.3 | CDS | 62260274 | 62260345 | 62260274 | ID=cds.MS.gene57543.t1;Parent=MS.gene57543.t1 |
| Gene Sequence |
| Protein sequence |
