AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57610.t1 MTR_4g126950 92.219 347 22 3 1 345 1 344 0.0 572
MS.gene57610.t1 MTR_5g033800 49.160 238 120 1 108 345 143 379 1.95e-91 278
MS.gene57610.t1 MTR_5g033800 49.160 238 120 1 108 345 143 379 1.71e-89 277
MS.gene57610.t1 MTR_7g109550 51.271 236 114 1 110 345 137 371 5.76e-86 267
MS.gene57610.t1 MTR_8g069790 47.679 237 123 1 109 345 177 412 1.57e-84 265
MS.gene57610.t1 MTR_0136s0010 44.444 261 143 1 85 345 147 405 1.98e-84 265
MS.gene57610.t1 MTR_7g093450 42.123 292 163 2 60 345 192 483 2.77e-84 266
MS.gene57610.t1 MTR_5g059400 41.812 287 156 3 59 345 135 410 4.57e-82 259
MS.gene57610.t1 MTR_1g116130 41.812 287 156 3 59 345 135 410 4.57e-82 259
MS.gene57610.t1 MTR_4g121560 42.324 241 137 2 107 345 111 351 3.76e-77 244
MS.gene57610.t1 MTR_7g094750 43.038 237 134 1 110 345 91 327 4.06e-71 224
MS.gene57610.t1 MTR_7g094750 43.038 237 134 1 110 345 91 327 1.62e-69 224
MS.gene57610.t1 MTR_2g022560 42.259 239 133 2 110 345 97 333 2.14e-68 221
MS.gene57610.t1 MTR_1g058380 41.224 245 139 3 105 345 50 293 2.42e-66 215
MS.gene57610.t1 MTR_3g090120 38.174 241 144 2 110 345 129 369 2.05e-63 209
MS.gene57610.t1 MTR_8g093530 37.500 240 149 1 107 345 87 326 4.21e-62 204
MS.gene57610.t1 MTR_5g071350 39.407 236 131 3 110 345 58 281 1.09e-59 197
MS.gene57610.t1 MTR_1g050448 37.553 237 136 3 108 344 51 275 1.81e-57 191
MS.gene57610.t1 MTR_3g464570 36.864 236 137 4 110 345 43 266 2.68e-56 187
MS.gene57610.t1 MTR_2g073510 36.820 239 137 5 108 345 31 256 3.05e-55 184
MS.gene57610.t1 MTR_5g034810 36.471 255 144 6 91 344 17 254 6.02e-55 184
MS.gene57610.t1 MTR_2g091175 37.083 240 132 4 109 345 49 272 7.13e-55 184
MS.gene57610.t1 MTR_1g112210 36.555 238 137 4 108 344 57 281 1.38e-53 181
MS.gene57610.t1 MTR_3g049330 36.626 243 135 4 106 345 27 253 2.92e-53 179
MS.gene57610.t1 MTR_3g021060 37.657 239 134 5 108 345 44 268 1.39e-52 177
MS.gene57610.t1 MTR_2g015720 36.441 236 136 5 110 345 42 263 2.40e-52 177
MS.gene57610.t1 MTR_4g016940 35.156 256 156 4 92 344 68 316 7.73e-52 177
MS.gene57610.t1 MTR_1g112210 35.294 238 127 4 108 344 57 268 1.85e-51 175
MS.gene57610.t1 MTR_4g088030 35.319 235 139 3 110 344 33 254 1.87e-51 174
MS.gene57610.t1 MTR_4g087730 34.298 242 140 4 107 345 42 267 2.05e-51 174
MS.gene57610.t1 MTR_2g073500 35.887 248 139 8 105 344 23 258 2.09e-51 174
MS.gene57610.t1 MTR_4g079700 33.898 236 143 4 110 345 47 269 5.70e-51 173
MS.gene57610.t1 MTR_3g021090 35.772 246 138 5 105 344 21 252 3.82e-49 170
MS.gene57610.t1 MTR_3g035830 34.310 239 142 4 108 345 29 253 1.11e-48 167
MS.gene57610.t1 MTR_7g068920 32.168 286 181 6 62 344 35 310 1.95e-48 168
MS.gene57610.t1 MTR_3g070900 34.286 245 151 4 104 345 100 337 1.12e-47 167
MS.gene57610.t1 MTR_1g111010 35.833 240 136 7 108 344 30 254 4.46e-47 163
MS.gene57610.t1 MTR_3g071650 35.983 239 141 5 110 344 89 319 7.41e-46 162
MS.gene57610.t1 MTR_5g083260 36.402 239 140 5 110 344 83 313 3.81e-44 157
MS.gene57610.t1 MTR_8g463790 33.054 239 146 5 110 345 71 298 7.76e-44 156
MS.gene57610.t1 MTR_4g014440 33.061 245 149 5 106 344 58 293 5.22e-43 154
MS.gene57610.t1 MTR_5g082880 34.137 249 147 6 105 345 160 399 6.08e-43 155
MS.gene57610.t1 MTR_4g014430 28.732 355 213 11 6 345 7 336 4.54e-42 152
MS.gene57610.t1 MTR_7g070060 31.707 246 153 5 105 344 59 295 1.05e-40 145
MS.gene57610.t1 MTR_7g070060 31.707 246 153 5 105 344 59 295 2.87e-39 144
MS.gene57610.t1 MTR_8g104970 29.248 359 207 12 12 345 19 355 9.84e-31 121
MS.gene57610.t1 MTR_2g022580 42.520 127 68 2 170 293 2 126 2.55e-30 112
MS.gene57610.t1 MTR_3g464570 34.302 172 101 4 174 345 3 162 8.79e-30 115
MS.gene57610.t1 MTR_5g035150 32.411 253 141 10 108 345 83 320 5.54e-29 116
MS.gene57610.t1 MTR_6g061710 28.958 259 157 6 73 319 131 374 1.17e-28 116
MS.gene57610.t1 MTR_8g027900 30.455 220 135 6 108 318 209 419 5.34e-28 114
MS.gene57610.t1 MTR_5g035090 33.486 218 127 6 109 319 82 288 1.13e-27 112
MS.gene57610.t1 MTR_1g076690 30.909 220 130 5 110 319 223 430 1.77e-27 113
MS.gene57610.t1 MTR_5g035080 32.524 206 123 5 99 303 64 254 2.13e-27 112
MS.gene57610.t1 MTR_6g016880 26.966 267 166 7 94 345 60 312 3.17e-27 111
MS.gene57610.t1 MTR_4g092830 29.032 248 154 11 110 345 48 285 6.83e-27 110
MS.gene57610.t1 MTR_7g019120 28.358 268 162 8 94 345 60 313 1.69e-26 109
MS.gene57610.t1 MTR_2g016060 32.530 249 142 10 111 345 56 292 1.75e-26 108
MS.gene57610.t1 MTR_7g033230 35.165 182 101 6 108 285 262 430 1.13e-25 108
MS.gene57610.t1 MTR_7g033230 35.165 182 101 6 108 285 332 500 1.65e-25 107
MS.gene57610.t1 MTR_3g109840 29.762 252 142 8 110 345 73 305 5.07e-25 105
MS.gene57610.t1 MTR_5g035070 31.765 255 136 8 110 345 87 322 7.22e-25 104
MS.gene57610.t1 MTR_5g035120 42.857 91 52 0 108 198 73 163 3.42e-24 97.8
MS.gene57610.t1 MTR_8g099300 26.361 349 185 12 10 345 19 308 1.12e-23 101
MS.gene57610.t1 MTR_7g019140 39.815 108 62 1 94 198 60 167 1.66e-23 95.9
MS.gene57610.t1 MTR_6g016875 29.600 250 154 7 108 345 62 301 6.61e-23 99.0
MS.gene57610.t1 MTR_6g007777 28.968 252 153 9 108 345 114 353 7.29e-23 99.4
MS.gene57610.t1 MTR_6g016240 29.249 253 154 11 110 345 71 315 1.28e-21 95.5
MS.gene57610.t1 MTR_8g093870 24.611 321 204 12 49 345 73 379 3.58e-19 88.6
MS.gene57610.t1 MTR_5g069320 31.410 156 80 4 165 320 156 284 8.90e-19 87.0
MS.gene57610.t1 MTR_4g125060 27.320 194 124 7 108 289 130 318 2.51e-18 86.3
MS.gene57610.t1 MTR_4g125060 27.320 194 124 7 108 289 198 386 3.33e-18 85.9
MS.gene57610.t1 MTR_4g125060 27.320 194 124 7 108 289 169 357 4.50e-18 85.5
MS.gene57610.t1 MTR_7g070060 27.660 188 122 4 162 344 15 193 5.32e-17 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57610.t1 AT5G20590 56.533 375 115 5 8 345 14 377 1.53e-150 429
MS.gene57610.t1 AT5G20590 56.533 375 115 5 8 345 14 377 1.02e-148 428
MS.gene57610.t1 AT3G62390 37.875 367 196 6 7 345 10 372 6.63e-92 281
MS.gene57610.t1 AT1G60790 48.617 253 128 1 95 345 175 427 2.99e-91 283
MS.gene57610.t1 AT3G62390 37.875 367 196 6 7 345 10 372 1.23e-90 279
MS.gene57610.t1 AT3G12060 46.502 243 130 0 103 345 189 431 2.09e-85 268
MS.gene57610.t1 AT5G06700 44.583 240 133 0 106 345 251 490 4.89e-83 263
MS.gene57610.t1 AT5G49340 43.725 247 137 2 101 345 91 337 1.69e-77 245
MS.gene57610.t1 AT5G19160 44.314 255 133 3 98 345 83 335 2.58e-76 239
MS.gene57610.t1 AT5G19160 44.314 255 133 3 98 345 83 335 1.56e-74 238
MS.gene57610.t1 AT3G06080 44.726 237 130 1 110 345 103 339 2.07e-74 234
MS.gene57610.t1 AT3G06080 44.726 237 130 1 110 345 103 339 3.67e-73 234
MS.gene57610.t1 AT1G48880 39.837 246 147 1 101 345 106 351 3.18e-69 223
MS.gene57610.t1 AT5G06230 37.551 245 146 2 108 345 60 304 4.79e-59 193
MS.gene57610.t1 AT5G06230 37.551 245 146 2 108 345 19 263 4.11e-58 192
MS.gene57610.t1 AT5G06230 37.551 245 146 2 108 345 60 304 6.28e-58 193
MS.gene57610.t1 AT2G31110 35.366 246 146 3 100 345 35 267 6.84e-58 191
MS.gene57610.t1 AT2G31110 34.836 244 146 3 100 343 35 265 5.26e-57 186
MS.gene57610.t1 AT2G30900 38.723 235 127 5 108 339 41 261 1.39e-56 185
MS.gene57610.t1 AT3G11570 39.352 216 130 1 131 345 103 318 1.76e-56 189
MS.gene57610.t1 AT2G30900 38.750 240 130 5 108 344 41 266 4.35e-56 187
MS.gene57610.t1 AT3G54260 36.226 265 141 6 91 341 20 270 3.08e-55 185
MS.gene57610.t1 AT5G58600 36.287 237 136 5 110 344 66 289 2.92e-54 180
MS.gene57610.t1 AT2G42570 34.524 252 150 4 94 345 33 269 1.06e-53 181
MS.gene57610.t1 AT5G58600 36.287 237 136 5 110 344 66 289 3.46e-53 180
MS.gene57610.t1 AT1G78710 34.146 246 147 5 101 345 29 260 3.06e-52 177
MS.gene57610.t1 AT3G55990 32.911 316 194 7 37 345 67 371 6.75e-52 179
MS.gene57610.t1 AT2G30010 37.917 240 134 6 109 345 55 282 1.02e-49 171
MS.gene57610.t1 AT2G30010 37.917 240 134 6 109 345 98 325 2.42e-49 171
MS.gene57610.t1 AT2G34070 33.333 237 145 3 110 345 65 289 3.92e-49 169
MS.gene57610.t1 AT2G40160 31.086 267 171 5 85 345 52 311 6.23e-47 161
MS.gene57610.t1 AT1G29050 31.799 239 145 3 110 345 61 284 1.20e-46 162
MS.gene57610.t1 AT3G14850 33.333 243 143 4 106 345 30 256 1.38e-46 162
MS.gene57610.t1 AT5G01360 33.068 251 154 5 99 344 25 266 4.24e-46 161
MS.gene57610.t1 AT2G40160 31.086 267 171 5 85 345 52 311 8.41e-46 161
MS.gene57610.t1 AT5G01360 33.068 251 154 5 99 344 81 322 1.20e-45 161
MS.gene57610.t1 AT2G40160 31.086 267 171 5 85 345 52 311 1.64e-45 161
MS.gene57610.t1 AT1G73140 33.846 260 151 7 89 345 53 294 4.12e-45 159
MS.gene57610.t1 AT2G40150 34.298 242 145 5 109 345 70 302 5.42e-44 157
MS.gene57610.t1 AT5G01620 32.377 244 145 5 108 344 108 338 6.39e-43 154
MS.gene57610.t1 AT5G01620 32.377 244 145 5 108 344 108 338 6.39e-43 154
MS.gene57610.t1 AT5G01620 32.377 244 145 5 108 344 116 346 7.51e-43 154
MS.gene57610.t1 AT3G11030 33.054 239 150 4 110 345 106 337 9.52e-43 154
MS.gene57610.t1 AT2G38320 30.894 246 149 5 108 344 57 290 1.31e-39 144
MS.gene57610.t1 AT2G40320 32.083 240 153 4 108 344 79 311 2.73e-38 141
MS.gene57610.t1 AT1G78710 32.673 202 120 6 101 300 22 209 1.01e-35 132
MS.gene57610.t1 AT1G01430 27.027 333 202 8 19 345 34 331 5.30e-32 125
MS.gene57610.t1 AT2G38320 30.516 213 128 4 140 344 7 207 4.46e-31 120
MS.gene57610.t1 AT5G15890 29.431 299 175 9 61 345 139 415 5.31e-31 123
MS.gene57610.t1 AT1G70230 30.435 276 144 8 64 319 38 285 9.59e-31 120
MS.gene57610.t1 AT4G25360 29.279 222 145 3 67 286 132 343 1.38e-29 119
MS.gene57610.t1 AT4G25360 29.279 222 145 3 67 286 132 343 1.38e-29 119
MS.gene57610.t1 AT5G51640 30.038 263 157 7 69 319 98 345 5.08e-29 117
MS.gene57610.t1 AT4G01080 29.218 243 153 5 109 345 90 319 5.73e-28 113
MS.gene57610.t1 AT5G64470 27.734 256 160 10 104 345 49 293 3.06e-27 109
MS.gene57610.t1 AT3G28150 34.536 194 112 6 99 284 54 240 4.92e-27 110
MS.gene57610.t1 AT5G64470 27.734 256 160 10 104 345 49 293 5.41e-27 108
MS.gene57610.t1 AT5G64470 28.000 250 155 10 110 345 55 293 1.35e-26 109
MS.gene57610.t1 AT5G64470 28.000 250 155 10 110 345 55 293 1.49e-26 109
MS.gene57610.t1 AT4G11090 32.653 196 117 7 95 285 66 251 2.79e-26 108
MS.gene57610.t1 AT5G64020 29.249 253 156 9 110 345 57 303 3.51e-26 108
MS.gene57610.t1 AT4G23790 32.663 199 121 7 91 285 62 251 5.18e-26 108
MS.gene57610.t1 AT2G37720 29.630 243 147 10 103 328 136 371 9.98e-25 104
MS.gene57610.t1 AT2G34070 30.539 167 103 3 180 345 1 155 1.90e-24 100
MS.gene57610.t1 AT2G14530 28.175 252 156 11 110 345 62 304 3.62e-23 99.8
MS.gene57610.t1 AT5G15900 26.154 260 167 8 99 345 75 322 5.17e-22 96.7
MS.gene57610.t1 AT3G02440 28.571 245 155 8 110 345 127 360 5.25e-22 97.1
MS.gene57610.t1 AT3G02440 28.571 245 155 8 110 345 135 368 5.35e-22 97.1
MS.gene57610.t1 AT5G15900 26.154 260 167 8 99 345 56 303 6.35e-22 96.3
MS.gene57610.t1 AT3G14850 30.178 169 99 4 180 345 1 153 1.32e-19 87.4
MS.gene57610.t1 AT2G31110 35.000 120 72 2 226 345 6 119 1.58e-19 86.3
MS.gene57610.t1 AT5G20680 27.426 237 156 6 60 289 171 398 2.07e-19 89.7
MS.gene57610.t1 AT5G20680 27.426 237 156 6 60 289 171 398 2.07e-19 89.7
MS.gene57610.t1 AT5G20680 27.426 237 156 6 60 289 171 398 2.07e-19 89.7
MS.gene57610.t1 AT5G20680 27.426 237 156 6 60 289 171 398 2.07e-19 89.7
MS.gene57610.t1 AT5G20680 27.426 237 156 6 60 289 153 380 2.69e-19 89.4
MS.gene57610.t1 AT5G20680 27.426 237 156 6 60 289 77 304 3.18e-19 88.6

Find 88 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTCAACTCCATCAAATTT+TGG 0.182186 8.4:+8126764 None:intergenic
GATATTCTTCTGCTCATTTC+AGG 0.196516 8.4:-8126661 None:intergenic
CTATAAAGACATGGGGAAAA+TGG 0.301251 8.4:-8126730 MS.gene57610:CDS
TATCTATATCCAAAATTTGA+TGG 0.330959 8.4:-8126773 MS.gene57610:CDS
CACACAGAATGGACTCGAAT+TGG 0.368869 8.4:+8128012 None:intergenic
GAATGGACTCGAATTGGTTT+CGG 0.393223 8.4:+8128018 None:intergenic
CAAGAACCTGGTTCATAAAT+CGG 0.407682 8.4:+8129305 None:intergenic
GTGGAGGATTGTTGCGGTGG+TGG 0.408628 8.4:+8129506 None:intergenic
AGAACAATGCGAGGAAGAAA+AGG 0.413314 8.4:+8129639 None:intergenic
TGATGCGATTCTAGCTTTGA+AGG 0.426591 8.4:-8129372 MS.gene57610:CDS
ACCGGGCACTGGTGGGCTCA+TGG 0.427472 8.4:-8127261 MS.gene57610:CDS
TCTTCAACACCGGGCACTGG+TGG 0.429397 8.4:-8127269 MS.gene57610:CDS
GATTGTTGCGGTGGTGGCGG+CGG 0.435023 8.4:+8129512 None:intergenic
GACATTCTCGTCTTCAACAC+CGG 0.435168 8.4:-8127279 MS.gene57610:CDS
ATGAGGTTTCCAGCGCCATT+TGG 0.439292 8.4:+8129223 None:intergenic
AGATCTGTGTGAGGAGAGAG+AGG 0.459272 8.4:+8129563 None:intergenic
TCCATGAGCCCACCAGTGCC+CGG 0.463009 8.4:+8127260 None:intergenic
ATCGACAGTGGCACCGTCAA+TGG 0.465554 8.4:+8129454 None:intergenic
GAGGATTGTTGCGGTGGTGG+CGG 0.467782 8.4:+8129509 None:intergenic
GCGATTTATACAATGGAACT+TGG 0.468450 8.4:-8129346 MS.gene57610:CDS
CAACCTCAACCCTCCATTGA+CGG 0.478982 8.4:-8129467 MS.gene57610:CDS
GTCAGCCCTCTTCCAGCGGT+TGG 0.489842 8.4:+8127299 None:intergenic
ATGATACTCATTATACCAAA+TGG 0.490084 8.4:-8129238 MS.gene57610:CDS
GCTTATGATTGCAAAATCAA+TGG 0.496546 8.4:-8129266 MS.gene57610:CDS
TTGAATGTCGGTAACGGTGG+TGG 0.500175 8.4:+8129487 None:intergenic
TGAGATCTCATTTCCTTGTG+AGG 0.501551 8.4:-8127392 MS.gene57610:CDS
GAAAGGTAAACAACTAATGC+TGG 0.505310 8.4:-8128057 MS.gene57610:CDS
TCGTCTTCAACACCGGGCAC+TGG 0.506054 8.4:-8127272 MS.gene57610:CDS
CTTCAACACCGGGCACTGGT+GGG 0.508299 8.4:-8127268 MS.gene57610:CDS
AAGGAATGCGATTTATACAA+TGG 0.511789 8.4:-8129353 MS.gene57610:CDS
CTCATTATACCAAATGGCGC+TGG 0.512407 8.4:-8129232 MS.gene57610:CDS
AGACGTCCAACCGCTGGAAG+AGG 0.517702 8.4:-8127305 MS.gene57610:CDS
GTGTGTACTTCGTGAAGGCT+TGG 0.524231 8.4:-8127994 MS.gene57610:CDS
TTCACGAAGTACACACAGAA+TGG 0.526243 8.4:+8128001 None:intergenic
GAGATCTCATTTCCTTGTGA+GGG 0.527350 8.4:-8127391 MS.gene57610:CDS
ATCTCATTTCCTTGTGAGGG+AGG 0.531071 8.4:-8127388 MS.gene57610:CDS
CATGGTCACACAATATCTAA+AGG 0.534276 8.4:-8127945 MS.gene57610:CDS
AATGTCGGTAACGGTGGTGG+AGG 0.538791 8.4:+8129490 None:intergenic
TCCAAAATTTGATGGAGTTG+AGG 0.539425 8.4:-8126765 MS.gene57610:CDS
TTGACAAGACGTCCAACCGC+TGG 0.543425 8.4:-8127311 MS.gene57610:CDS
AGAATAAATTACTATAAAGA+AGG 0.546605 8.4:-8126800 MS.gene57610:CDS
CACACAATATCTAAAGGAAG+AGG 0.548764 8.4:-8127939 MS.gene57610:CDS
ATCCTACATTGTCAATAGAT+CGG 0.548798 8.4:-8127335 MS.gene57610:CDS
TTACTGTAGGTTTAGTGCCA+AGG 0.554186 8.4:-8128096 MS.gene57610:intron
AAATGGCGCTGGAAACCTCA+TGG 0.554636 8.4:-8129221 MS.gene57610:CDS
AAAGGGTCGCGACAGTGTAA+CGG 0.558790 8.4:+8129657 None:intergenic
CGACAGTGTAACGGTTATTG+TGG 0.561678 8.4:+8129666 None:intergenic
ATACAATGGAACTTGGGTAG+AGG 0.561813 8.4:-8129339 MS.gene57610:CDS
TCTCATTTCCTTGTGAGGGA+GGG 0.571728 8.4:-8127387 MS.gene57610:CDS
CGATTTATACAATGGAACTT+GGG 0.573614 8.4:-8129345 MS.gene57610:CDS
CAGAAAGGCTATAAAGACAT+GGG 0.576036 8.4:-8126738 MS.gene57610:CDS
CTCATGGATGTGATCTTCCA+AGG 0.576806 8.4:-8129205 MS.gene57610:intron
GACAGTGGCACCGTCAATGG+AGG 0.578822 8.4:+8129457 None:intergenic
ATTCTGTGTGTACTTCGTGA+AGG 0.579237 8.4:-8127999 MS.gene57610:CDS
ATGATTGCAAAATCAATGGA+AGG 0.581693 8.4:-8129262 MS.gene57610:CDS
GACGTCCAACCGCTGGAAGA+GGG 0.584315 8.4:-8127304 MS.gene57610:CDS
ATCCGATCTATTGACAATGT+AGG 0.584511 8.4:+8127333 None:intergenic
GATTATCCGATTTATGAACC+AGG 0.585634 8.4:-8129311 MS.gene57610:CDS
AGCAAAATGTATGAGATACA+TGG 0.585784 8.4:-8127963 MS.gene57610:CDS
TCATAAGCTTCATCAACGTA+AGG 0.586089 8.4:+8129281 None:intergenic
AAGCAGATTGTCTACTATCG+TGG 0.591772 8.4:-8126683 MS.gene57610:CDS
TGAGCAGGACTATGATTGTA+CGG 0.591789 8.4:-8127424 MS.gene57610:intron
ACATTCTCGTCTTCAACACC+GGG 0.594424 8.4:-8127278 MS.gene57610:CDS
TTGAGGTTGAATGTCGGTAA+CGG 0.595881 8.4:+8129481 None:intergenic
GAACAATGCGAGGAAGAAAA+GGG 0.596574 8.4:+8129640 None:intergenic
TGGAGTTGAGGCATACAGAA+AGG 0.596745 8.4:-8126753 MS.gene57610:CDS
GGTAAACAACTAATGCTGGT+AGG 0.608787 8.4:-8128053 MS.gene57610:CDS
ACAGAAAGGCTATAAAGACA+TGG 0.612533 8.4:-8126739 MS.gene57610:CDS
GTCTGTATTCGTAACAGCAA+CGG 0.613924 8.4:+8129406 None:intergenic
GCACCGTCAATGGAGGGTTG+AGG 0.617478 8.4:+8129464 None:intergenic
TACGAACTCCCTCCCTCACA+AGG 0.618151 8.4:+8127379 None:intergenic
TCAACGTAAGGACAAGAACC+TGG 0.622466 8.4:+8129293 None:intergenic
TAGAGATAGAGATCTGTGTG+AGG 0.623061 8.4:+8129554 None:intergenic
GGAGTTCGTATGAATGCACA+AGG 0.632015 8.4:-8127366 MS.gene57610:CDS
GGTGGCGGCGGAGAGAGAGA+AGG 0.634925 8.4:+8129524 None:intergenic
GTGGTGGAGGATTGTTGCGG+TGG 0.637075 8.4:+8129503 None:intergenic
TTGTGAGAAAGAACAATGCG+AGG 0.647296 8.4:+8129630 None:intergenic
ACAGTGGCACCGTCAATGGA+GGG 0.652711 8.4:+8129458 None:intergenic
GAATGTCAGCCCTCTTCCAG+CGG 0.657009 8.4:+8127295 None:intergenic
AGAAAGGCTATAAAGACATG+GGG 0.676470 8.4:-8126737 MS.gene57610:CDS
ATGATCATCGACATCGACAG+TGG 0.680354 8.4:+8129442 None:intergenic
TGTGTGAGGAGAGAGAGGAG+TGG 0.682320 8.4:+8129568 None:intergenic
AGGTTGAATGTCGGTAACGG+TGG 0.682616 8.4:+8129484 None:intergenic
GGAGGGTTGAGGTTGAATGT+CGG 0.682787 8.4:+8129475 None:intergenic
TGCTGTTACGAATACAGACG+AGG 0.687566 8.4:-8129402 MS.gene57610:CDS
ACGGTGGTGGAGGATTGTTG+CGG 0.694908 8.4:+8129500 None:intergenic
CTTGGAAGATCACATCCATG+AGG 0.719148 8.4:+8129206 None:intergenic
GAGTTCGTATGAATGCACAA+GGG 0.808659 8.4:-8127365 MS.gene57610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATACTTACAAAATGATTA+AGG - chr8.4:8128804-8128823 MS.gene57610:intron 15.0%
!! AGAATAAATTACTATAAAGA+AGG - chr8.4:8129536-8129555 MS.gene57610:CDS 15.0%
!!! AATAAAAACTAATTTGTGAA+AGG + chr8.4:8129406-8129425 None:intergenic 15.0%
!!! ATAAAAACTAATTTGTGAAA+GGG + chr8.4:8129405-8129424 None:intergenic 15.0%
!!! ATGTTTATCTTGTTAAATTT+TGG - chr8.4:8129372-8129391 MS.gene57610:CDS 15.0%
!! AACATGGTCAAAAAATAAAA+AGG - chr8.4:8129216-8129235 MS.gene57610:CDS 20.0%
!! ACATGCTACTATTTATTAAA+CGG - chr8.4:8127311-8127330 MS.gene57610:CDS 20.0%
!! ATGTTAGAATTCAATAAACA+TGG + chr8.4:8129201-8129220 None:intergenic 20.0%
!! ATGTTTATTGAATTCTAACA+TGG - chr8.4:8129200-8129219 MS.gene57610:intron 20.0%
!! GAAAATAATAAAATCTGGAT+GGG - chr8.4:8128852-8128871 MS.gene57610:intron 20.0%
!! TAATAAGTAGTTCATGTAAT+TGG + chr8.4:8128531-8128550 None:intergenic 20.0%
!! TATATGTCAAATGATTAAGA+TGG - chr8.4:8127813-8127832 MS.gene57610:intron 20.0%
!! TATCTATATCCAAAATTTGA+TGG - chr8.4:8129563-8129582 MS.gene57610:CDS 20.0%
!! TGAAAATAATAAAATCTGGA+TGG - chr8.4:8128851-8128870 MS.gene57610:intron 20.0%
!! TGTTAGAATTCAATAAACAT+GGG + chr8.4:8129200-8129219 None:intergenic 20.0%
!!! ATAAAGTACTGTCTATATTT+TGG - chr8.4:8128756-8128775 MS.gene57610:intron 20.0%
!!! CAGATTTTATTATTTTCACA+GGG + chr8.4:8128849-8128868 None:intergenic 20.0%
! AATTGTTTCCACAAATAGAA+TGG + chr8.4:8128009-8128028 None:intergenic 25.0%
! ATCAACCGATTATATGTATT+AGG - chr8.4:8129325-8129344 MS.gene57610:CDS 25.0%
! ATGATACTCATTATACCAAA+TGG - chr8.4:8127098-8127117 MS.gene57610:intron 25.0%
! ATGTTCATAAATAGGATTCA+AGG - chr8.4:8127264-8127283 MS.gene57610:CDS 25.0%
! CCTGTGAAAATAATAAAATC+TGG - chr8.4:8128847-8128866 MS.gene57610:intron 25.0%
! GAAATTATTTACCATGTTTG+CGG - chr8.4:8127492-8127511 MS.gene57610:intron 25.0%
! GTCTGTCAAAAATAAACAAT+GGG - chr8.4:8129470-8129489 MS.gene57610:CDS 25.0%
! GTTAGAATTCAATAAACATG+GGG + chr8.4:8129199-8129218 None:intergenic 25.0%
! GTTATCATGATAATAATGAG+TGG - chr8.4:8127578-8127597 MS.gene57610:intron 25.0%
! TAAATAGGATTCAAGGAATT+TGG - chr8.4:8127271-8127290 MS.gene57610:CDS 25.0%
! TCAATGTGATGTTCATAAAT+AGG - chr8.4:8127256-8127275 MS.gene57610:CDS 25.0%
! TCTTGCCTAATACATATAAT+CGG + chr8.4:8129333-8129352 None:intergenic 25.0%
! TTAGAATTCAATAAACATGG+GGG + chr8.4:8129198-8129217 None:intergenic 25.0%
!! ATCATGCTTTTCAATTTTCA+TGG + chr8.4:8127923-8127942 None:intergenic 25.0%
!! CCAGATTTTATTATTTTCAC+AGG + chr8.4:8128850-8128869 None:intergenic 25.0%
!! GGAAACAATTTTAAACTTGT+GGG - chr8.4:8128019-8128038 MS.gene57610:CDS 25.0%
!! TCAAATGATTAAGATGGATT+TGG - chr8.4:8127819-8127838 MS.gene57610:intron 25.0%
!! TCATGCTTTTCAATTTTCAT+GGG + chr8.4:8127922-8127941 None:intergenic 25.0%
!! TGGAAACAATTTTAAACTTG+TGG - chr8.4:8128018-8128037 MS.gene57610:CDS 25.0%
!! TGTTCATTATGCTAAGATTT+TGG - chr8.4:8128883-8128902 MS.gene57610:intron 25.0%
!!! AAGGAATTTGGTTTGATTTA+GGG - chr8.4:8127283-8127302 MS.gene57610:CDS 25.0%
!!! ATGAATTCAATGTCATTTTC+TGG + chr8.4:8128707-8128726 None:intergenic 25.0%
!!! CTACTTTGTTTTCAAATTTG+AGG - chr8.4:8128420-8128439 MS.gene57610:intron 25.0%
!!! GGTGGATTAAATTAAATTCT+GGG - chr8.4:8127513-8127532 MS.gene57610:intron 25.0%
!!! TGAATTCAATGTCATTTTCT+GGG + chr8.4:8128706-8128725 None:intergenic 25.0%
AAGGAATGCGATTTATACAA+TGG - chr8.4:8126983-8127002 MS.gene57610:intron 30.0%
ACAGTAATATGTGAAAGAAG+TGG + chr8.4:8128227-8128246 None:intergenic 30.0%
AGATTTGAATCCATTAGAGA+AGG - chr8.4:8127452-8127471 MS.gene57610:intron 30.0%
AGCAAAATGTATGAGATACA+TGG - chr8.4:8128373-8128392 MS.gene57610:intron 30.0%
ATCCTACATTGTCAATAGAT+CGG - chr8.4:8129001-8129020 MS.gene57610:intron 30.0%
ATGATAATAATGAGTGGACA+GGG - chr8.4:8127584-8127603 MS.gene57610:intron 30.0%
ATGATTGCAAAATCAATGGA+AGG - chr8.4:8127074-8127093 MS.gene57610:intron 30.0%
CCAAGAGAAGTTACAAATAT+AGG + chr8.4:8129276-8129295 None:intergenic 30.0%
CCTATATTTGTAACTTCTCT+TGG - chr8.4:8129273-8129292 MS.gene57610:CDS 30.0%
CCTGTCTAAATAACTTAAGA+CGG + chr8.4:8127698-8127717 None:intergenic 30.0%
CGATTTATACAATGGAACTT+GGG - chr8.4:8126991-8127010 MS.gene57610:intron 30.0%
CGTCTGTCAAAAATAAACAA+TGG - chr8.4:8129469-8129488 MS.gene57610:CDS 30.0%
CTTCTTTCACATATTACTGT+AGG - chr8.4:8128227-8128246 MS.gene57610:intron 30.0%
GCTTATGATTGCAAAATCAA+TGG - chr8.4:8127070-8127089 MS.gene57610:intron 30.0%
GTTGAAAATGACATCACAAA+CGG + chr8.4:8127194-8127213 None:intergenic 30.0%
TAATATGTGAAAGAAGTGGA+CGG + chr8.4:8128223-8128242 None:intergenic 30.0%
TGATTGTGTACATTCAATTC+AGG - chr8.4:8129145-8129164 MS.gene57610:intron 30.0%
TTTGTAAGTTTGTCTCTTCA+AGG - chr8.4:8128598-8128617 MS.gene57610:intron 30.0%
! TTATTTTCACAGGGAAATGA+TGG + chr8.4:8128840-8128859 None:intergenic 30.0%
!! AAAAGCATTATGCAGAAAGT+TGG - chr8.4:8127640-8127659 MS.gene57610:intron 30.0%
!! CAAAACACAGTAAGTTTTGA+AGG + chr8.4:8127964-8127983 None:intergenic 30.0%
!! CAAGGAATTTGGTTTGATTT+AGG - chr8.4:8127282-8127301 MS.gene57610:CDS 30.0%
!! CGGTGGATTAAATTAAATTC+TGG - chr8.4:8127512-8127531 MS.gene57610:intron 30.0%
!!! CAGACGACTTGTTAATTTTA+TGG + chr8.4:8129455-8129474 None:intergenic 30.0%
!!! TAGTAGACAATCTGCTTTTT+TGG + chr8.4:8129650-8129669 None:intergenic 30.0%
AATTCAAATTGAAGAGCTCC+AGG - chr8.4:8127883-8127902 MS.gene57610:intron 35.0%
AATTCATTAGAGAGTGACCT+TGG + chr8.4:8127151-8127170 None:intergenic 35.0%
AATTTAATCCACCGCAAACA+TGG + chr8.4:8127506-8127525 None:intergenic 35.0%
ACAGAAAGGCTATAAAGACA+TGG - chr8.4:8129597-8129616 MS.gene57610:CDS 35.0%
AGAAAGGCTATAAAGACATG+GGG - chr8.4:8129599-8129618 MS.gene57610:CDS 35.0%
ATAACGCGATTTGATTGTCA+AGG + chr8.4:8127415-8127434 None:intergenic 35.0%
ATCCGATCTATTGACAATGT+AGG + chr8.4:8129006-8129025 None:intergenic 35.0%
ATTATTTACCATGTTTGCGG+TGG - chr8.4:8127495-8127514 MS.gene57610:intron 35.0%
CAAGAACCTGGTTCATAAAT+CGG + chr8.4:8127034-8127053 None:intergenic 35.0%
CACACAATATCTAAAGGAAG+AGG - chr8.4:8128397-8128416 MS.gene57610:intron 35.0%
CAGAAAGGCTATAAAGACAT+GGG - chr8.4:8129598-8129617 MS.gene57610:CDS 35.0%
CATGATAATAATGAGTGGAC+AGG - chr8.4:8127583-8127602 MS.gene57610:intron 35.0%
CATGGTCACACAATATCTAA+AGG - chr8.4:8128391-8128410 MS.gene57610:intron 35.0%
CCGTCTTAAGTTATTTAGAC+AGG - chr8.4:8127695-8127714 MS.gene57610:intron 35.0%
CTATAAAGACATGGGGAAAA+TGG - chr8.4:8129606-8129625 MS.gene57610:CDS 35.0%
GAAAGGTAAACAACTAATGC+TGG - chr8.4:8128279-8128298 MS.gene57610:intron 35.0%
GATTATCCGATTTATGAACC+AGG - chr8.4:8127025-8127044 MS.gene57610:intron 35.0%
GCGATTTATACAATGGAACT+TGG - chr8.4:8126990-8127009 MS.gene57610:intron 35.0%
GGAATTGACCATTCTATTTG+TGG - chr8.4:8127998-8128017 MS.gene57610:CDS 35.0%
GTACATTCAATTCAGGAAAC+TGG - chr8.4:8129152-8129171 MS.gene57610:intron 35.0%
GTCAATTCCTGAAAAATTGC+TGG + chr8.4:8127987-8128006 None:intergenic 35.0%
TAACGCGATTTGATTGTCAA+GGG + chr8.4:8127414-8127433 None:intergenic 35.0%
TATTCAACAGCCTTCTCTAA+TGG + chr8.4:8127465-8127484 None:intergenic 35.0%
TCATAAGCTTCATCAACGTA+AGG + chr8.4:8127058-8127077 None:intergenic 35.0%
TCCAAAATTTGATGGAGTTG+AGG - chr8.4:8129571-8129590 MS.gene57610:CDS 35.0%
TTATAGAGGGAAGATTGGAT+TGG - chr8.4:8127744-8127763 MS.gene57610:intron 35.0%
TTGTCTCTTCAAGGTTTGAT+GGG - chr8.4:8128607-8128626 MS.gene57610:intron 35.0%
TTTGTCTCTTCAAGGTTTGA+TGG - chr8.4:8128606-8128625 MS.gene57610:intron 35.0%
! AAGCATAGCTTCTTTTGATG+TGG - chr8.4:8128061-8128080 MS.gene57610:CDS 35.0%
! AGTTTTTATTCCCTGCTATG+TGG - chr8.4:8129416-8129435 MS.gene57610:CDS 35.0%
! GTTCGGTAACTGTTGATAAA+TGG - chr8.4:8127776-8127795 MS.gene57610:intron 35.0%
! TGTTTTGCCAGCAATTTTTC+AGG - chr8.4:8127977-8127996 MS.gene57610:CDS 35.0%
! TTTTCCCAACATTGAACTCA+AGG + chr8.4:8127549-8127568 None:intergenic 35.0%
!! TGGATTGGTAGTAGATAGTT+CGG - chr8.4:8127759-8127778 MS.gene57610:intron 35.0%
!!! CAGTAAGTTTTGAAGGAACA+CGG + chr8.4:8127957-8127976 None:intergenic 35.0%
AAGCAGATTGTCTACTATCG+TGG - chr8.4:8129653-8129672 MS.gene57610:CDS 40.0%
ACTAACAGCTGAGAAGCAAA+GGG + chr8.4:8128167-8128186 None:intergenic 40.0%
AGAACAATGCGAGGAAGAAA+AGG + chr8.4:8126700-8126719 None:intergenic 40.0%
AGAGCCTTGAGTTCAATGTT+GGG - chr8.4:8127542-8127561 MS.gene57610:intron 40.0%
AGCATTATGCAGAAAGTTGG+AGG - chr8.4:8127643-8127662 MS.gene57610:intron 40.0%
ATACAATGGAACTTGGGTAG+AGG - chr8.4:8126997-8127016 MS.gene57610:intron 40.0%
ATGGGTTTGAGTATGATTCC+TGG + chr8.4:8127904-8127923 None:intergenic 40.0%
ATTCTGTGTGTACTTCGTGA+AGG - chr8.4:8128337-8128356 MS.gene57610:intron 40.0%
CTGCATTATAGAGGGAAGAT+TGG - chr8.4:8127739-8127758 MS.gene57610:intron 40.0%
GAAAGACAAACCACATAGCA+GGG + chr8.4:8129429-8129448 None:intergenic 40.0%
GAACAATGCGAGGAAGAAAA+GGG + chr8.4:8126699-8126718 None:intergenic 40.0%
GAGATCTCATTTCCTTGTGA+GGG - chr8.4:8128945-8128964 MS.gene57610:intron 40.0%
GAGTTCGTATGAATGCACAA+GGG - chr8.4:8128971-8128990 MS.gene57610:intron 40.0%
GGTAAACAACTAATGCTGGT+AGG - chr8.4:8128283-8128302 MS.gene57610:intron 40.0%
GTCTGTATTCGTAACAGCAA+CGG + chr8.4:8126933-8126952 None:intergenic 40.0%
TACTAACAGCTGAGAAGCAA+AGG + chr8.4:8128168-8128187 None:intergenic 40.0%
TAGAGATAGAGATCTGTGTG+AGG + chr8.4:8126785-8126804 None:intergenic 40.0%
TAGAGCCTTGAGTTCAATGT+TGG - chr8.4:8127541-8127560 MS.gene57610:intron 40.0%
TGAAAGACAAACCACATAGC+AGG + chr8.4:8129430-8129449 None:intergenic 40.0%
TGAGATCTCATTTCCTTGTG+AGG - chr8.4:8128944-8128963 MS.gene57610:intron 40.0%
TGAGCAGGACTATGATTGTA+CGG - chr8.4:8128912-8128931 MS.gene57610:intron 40.0%
TTACTGTAGGTTTAGTGCCA+AGG - chr8.4:8128240-8128259 MS.gene57610:intron 40.0%
TTCACGAAGTACACACAGAA+TGG + chr8.4:8128338-8128357 None:intergenic 40.0%
TTGAGGTTGAATGTCGGTAA+CGG + chr8.4:8126858-8126877 None:intergenic 40.0%
TTGTGAGAAAGAACAATGCG+AGG + chr8.4:8126709-8126728 None:intergenic 40.0%
! ATGTAAGCGCTGCATTATAG+AGG - chr8.4:8127730-8127749 MS.gene57610:intron 40.0%
! GAATGGACTCGAATTGGTTT+CGG + chr8.4:8128321-8128340 None:intergenic 40.0%
! GCCTCAACTCCATCAAATTT+TGG + chr8.4:8129575-8129594 None:intergenic 40.0%
! GTTTAGTGCCAAGGACTTTT+TGG - chr8.4:8128249-8128268 MS.gene57610:intron 40.0%
! TGTAAGCGCTGCATTATAGA+GGG - chr8.4:8127731-8127750 MS.gene57610:intron 40.0%
!! GACTTTTTGGCAAGACTGAA+AGG - chr8.4:8128262-8128281 MS.gene57610:intron 40.0%
!! TGATGCGATTCTAGCTTTGA+AGG - chr8.4:8126964-8126983 MS.gene57610:intron 40.0%
!! TTATTAAACGGTGTTACCGC+AGG - chr8.4:8127323-8127342 MS.gene57610:CDS 40.0%
!! TTCAGGTTTATTTCTGCGAG+TGG - chr8.4:8127361-8127380 MS.gene57610:CDS 40.0%
ACATTCTCGTCTTCAACACC+GGG - chr8.4:8129058-8129077 MS.gene57610:intron 45.0%
ATCAAATCGCGTTATTGCGC+TGG - chr8.4:8127420-8127439 MS.gene57610:CDS 45.0%
ATCTCATTTCCTTGTGAGGG+AGG - chr8.4:8128948-8128967 MS.gene57610:intron 45.0%
ATGATCATCGACATCGACAG+TGG + chr8.4:8126897-8126916 None:intergenic 45.0%
CAGTCTTGCCAAAAAGTCCT+TGG + chr8.4:8128260-8128279 None:intergenic 45.0%
CGACAGTGTAACGGTTATTG+TGG + chr8.4:8126673-8126692 None:intergenic 45.0%
CTCATGGAAAAACTGCTCGA+GGG - chr8.4:8129091-8129110 MS.gene57610:intron 45.0%
CTCATGGATGTGATCTTCCA+AGG - chr8.4:8127131-8127150 MS.gene57610:intron 45.0%
CTCATTATACCAAATGGCGC+TGG - chr8.4:8127104-8127123 MS.gene57610:intron 45.0%
CTTGGAAGATCACATCCATG+AGG + chr8.4:8127133-8127152 None:intergenic 45.0%
GACATTCTCGTCTTCAACAC+CGG - chr8.4:8129057-8129076 MS.gene57610:intron 45.0%
GGAGTTCGTATGAATGCACA+AGG - chr8.4:8128970-8128989 MS.gene57610:intron 45.0%
TCAACGTAAGGACAAGAACC+TGG + chr8.4:8127046-8127065 None:intergenic 45.0%
TCTCATTTCCTTGTGAGGGA+GGG - chr8.4:8128949-8128968 MS.gene57610:intron 45.0%
TGCTGTTACGAATACAGACG+AGG - chr8.4:8126934-8126953 MS.gene57610:intron 45.0%
TGGAGTTGAGGCATACAGAA+AGG - chr8.4:8129583-8129602 MS.gene57610:CDS 45.0%
! CACACAGAATGGACTCGAAT+TGG + chr8.4:8128327-8128346 None:intergenic 45.0%
!! AGGTTTATTTCTGCGAGTGG+AGG - chr8.4:8127364-8127383 MS.gene57610:CDS 45.0%
AAAGGGTCGCGACAGTGTAA+CGG + chr8.4:8126682-8126701 None:intergenic 50.0%
AGATCTGTGTGAGGAGAGAG+AGG + chr8.4:8126776-8126795 None:intergenic 50.0%
ATCGAACTAAACGACGCCTG+CGG + chr8.4:8127342-8127361 None:intergenic 50.0%
ATGAGGTTTCCAGCGCCATT+TGG + chr8.4:8127116-8127135 None:intergenic 50.0%
CAACCTCAACCCTCCATTGA+CGG - chr8.4:8126869-8126888 MS.gene57610:intron 50.0%
GCTCATGGAAAAACTGCTCG+AGG - chr8.4:8129090-8129109 MS.gene57610:intron 50.0%
GGAAAAACTGCTCGAGGGTT+AGG - chr8.4:8129096-8129115 MS.gene57610:intron 50.0%
GGAGGGTTGAGGTTGAATGT+CGG + chr8.4:8126864-8126883 None:intergenic 50.0%
GTGTGTACTTCGTGAAGGCT+TGG - chr8.4:8128342-8128361 MS.gene57610:intron 50.0%
! AAATGGCGCTGGAAACCTCA+TGG - chr8.4:8127115-8127134 MS.gene57610:intron 50.0%
! AGGTTGAATGTCGGTAACGG+TGG + chr8.4:8126855-8126874 None:intergenic 50.0%
! GGCGTCGTTTAGTTCGATTC+AGG - chr8.4:8127344-8127363 MS.gene57610:CDS 50.0%
! TTGAATGTCGGTAACGGTGG+TGG + chr8.4:8126852-8126871 None:intergenic 50.0%
!! AGTTGGAGGAGCACTTGTGA+TGG - chr8.4:8127657-8127676 MS.gene57610:intron 50.0%
!!! AGATTTTGGAGCGCTTGAGC+AGG - chr8.4:8128897-8128916 MS.gene57610:intron 50.0%
ACAGTGGCACCGTCAATGGA+GGG + chr8.4:8126881-8126900 None:intergenic 55.0%
ATCGACAGTGGCACCGTCAA+TGG + chr8.4:8126885-8126904 None:intergenic 55.0%
GAATGTCAGCCCTCTTCCAG+CGG + chr8.4:8129044-8129063 None:intergenic 55.0%
TACGAACTCCCTCCCTCACA+AGG + chr8.4:8128960-8128979 None:intergenic 55.0%
TGTGTGAGGAGAGAGAGGAG+TGG + chr8.4:8126771-8126790 None:intergenic 55.0%
TTGACAAGACGTCCAACCGC+TGG - chr8.4:8129025-8129044 MS.gene57610:intron 55.0%
! AATGTCGGTAACGGTGGTGG+AGG + chr8.4:8126849-8126868 None:intergenic 55.0%
!! ACGGTGGTGGAGGATTGTTG+CGG + chr8.4:8126839-8126858 None:intergenic 55.0%
AGACGTCCAACCGCTGGAAG+AGG - chr8.4:8129031-8129050 MS.gene57610:intron 60.0%
GACAGTGGCACCGTCAATGG+AGG + chr8.4:8126882-8126901 None:intergenic 60.0%
GACGTCCAACCGCTGGAAGA+GGG - chr8.4:8129032-8129051 MS.gene57610:intron 60.0%
GCACCGTCAATGGAGGGTTG+AGG + chr8.4:8126875-8126894 None:intergenic 60.0%
GTGGAGGATTGTTGCGGTGG+TGG + chr8.4:8126833-8126852 None:intergenic 60.0%
TCGTCTTCAACACCGGGCAC+TGG - chr8.4:8129064-8129083 MS.gene57610:intron 60.0%
! GAGGATTGTTGCGGTGGTGG+CGG + chr8.4:8126830-8126849 None:intergenic 60.0%
! GTGGTGGAGGATTGTTGCGG+TGG + chr8.4:8126836-8126855 None:intergenic 60.0%
!! CTTCAACACCGGGCACTGGT+GGG - chr8.4:8129068-8129087 MS.gene57610:intron 60.0%
!! TCTTCAACACCGGGCACTGG+TGG - chr8.4:8129067-8129086 MS.gene57610:intron 60.0%
GTCAGCCCTCTTCCAGCGGT+TGG + chr8.4:8129040-8129059 None:intergenic 65.0%
TCCATGAGCCCACCAGTGCC+CGG + chr8.4:8129079-8129098 None:intergenic 65.0%
!! GATTGTTGCGGTGGTGGCGG+CGG + chr8.4:8126827-8126846 None:intergenic 65.0%
! GGTGGCGGCGGAGAGAGAGA+AGG + chr8.4:8126815-8126834 None:intergenic 70.0%
!! ACCGGGCACTGGTGGGCTCA+TGG - chr8.4:8129075-8129094 MS.gene57610:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 8126662 8129696 8126662 ID=MS.gene57610
chr8.4 mRNA 8126662 8129696 8126662 ID=MS.gene57610.t1;Parent=MS.gene57610
chr8.4 exon 8129206 8129696 8129206 ID=MS.gene57610.t1.exon1;Parent=MS.gene57610.t1
chr8.4 CDS 8129206 8129696 8129206 ID=cds.MS.gene57610.t1;Parent=MS.gene57610.t1
chr8.4 exon 8127917 8128109 8127917 ID=MS.gene57610.t1.exon2;Parent=MS.gene57610.t1
chr8.4 CDS 8127917 8128109 8127917 ID=cds.MS.gene57610.t1;Parent=MS.gene57610.t1
chr8.4 exon 8127246 8127439 8127246 ID=MS.gene57610.t1.exon3;Parent=MS.gene57610.t1
chr8.4 CDS 8127246 8127439 8127246 ID=cds.MS.gene57610.t1;Parent=MS.gene57610.t1
chr8.4 exon 8126662 8126820 8126662 ID=MS.gene57610.t1.exon4;Parent=MS.gene57610.t1
chr8.4 CDS 8126662 8126820 8126662 ID=cds.MS.gene57610.t1;Parent=MS.gene57610.t1
Gene Sequence

>MS.gene57610

ATGATTTTCCACAATAACCGTTACACTGTCGCGACCCTTTTCTTCCTCGCATTGTTCTTTCTCACAATTTTCTTCTATTCATTCTTCTTCTTCTTCAACACAACAACCACTCCTCTCTCTCCTCACACAGATCTCTATCTCTATATCAAACCTTCTCTCTCTCCGCCGCCACCACCGCAACAATCCTCCACCACCGTTACCGACATTCAACCTCAACCCTCCATTGACGGTGCCACTGTCGATGTCGATGATCATGAATCTGTTGATTCCGTTGCTGTTACGAATACAGACGAGGTTGATGATGATGCGATTCTAGCTTTGAAGGAATGCGATTTATACAATGGAACTTGGGTAGAGGATGAAGATTATCCGATTTATGAACCAGGTTCTTGTCCTTACGTTGATGAAGCTTATGATTGCAAAATCAATGGAAGGAATGATACTCATTATACCAAATGGCGCTGGAAACCTCATGGATGTGATCTTCCAAGGTCACTCTCTAATGAATTTCTTAATCGATTTGTTTGTTCCGTTTGTGATGTCATTTTCAACTATTTGCATGTAGCTGTGTTTATGTTTGATTAGTTCATTTGTTCAATGTGATGTTCATAAATAGGATTCAAGGAATTTGGTTTGATTTAGGGATAAAACATGCTACTATTTATTAAACGGTGTTACCGCAGGCGTCGTTTAGTTCGATTCAGGTTTATTTCTGCGAGTGGAGGAACAACTTGATAGTTAGTATACAACCCTTGACAATCAAATCGCGTTATTGCGCTGGTCATTGTTAAGATTTGAATCCATTAGAGAAGGCTGTTGAATAATCTAAAGAAATTATTTACCATGTTTGCGGTGGATTAAATTAAATTCTGGGTAATATAGAGCCTTGAGTTCAATGTTGGGAAAAAGTGTAAATGTTATCATGATAATAATGAGTGGACAGGGCAAGATGTTGATTAGAAACGAGAAATTGTAAATAAAAGCATTATGCAGAAAGTTGGAGGAGCACTTGTGATGGAAAAGAAGTTACATACCGTCTTAAGTTATTTAGACAGGAATGAAGAATACATGTAAGCGCTGCATTATAGAGGGAAGATTGGATTGGTAGTAGATAGTTCGGTAACTGTTGATAAATGGAGACTAATGAAAACTATATGTCAAATGATTAAGATGGATTTGGATTTAAATGAATTTTTAGTATCATGACTTTAGCTTCAAGCTAATTCAAATTGAAGAGCTCCAGGAATCATACTCAAACCCATGAAAATTGAAAAGCATGATCAACTAGTTAGCCGTGTTCCTTCAAAACTTACTGTGTTTTGCCAGCAATTTTTCAGGAATTGACCATTCTATTTGTGGAAACAATTTTAAACTTGTGGGTACATTTATGAAAAGTTTCAAGCATAGCTTCTTTTGATGTGGTGAGAAAAATTAAGTTGTTGTCTAGCAAATGACAAAAATAGCAAAAGAGTCATAATTCAATAGTGTTTTTCAAACATCTACCCTTTGCTTCTCAGCTGTTAGTAGTTTGCTGTAAAGTTAGTTATTTACATTTTCTCCGTCCACTTCTTTCACATATTACTGTAGGTTTAGTGCCAAGGACTTTTTGGCAAGACTGAAAGGTAAACAACTAATGCTGGTAGGAGATTCTATGAACCGAAACCAATTCGAGTCCATTCTGTGTGTACTTCGTGAAGGCTTGGACAATAAGAGCAAAATGTATGAGATACATGGTCACACAATATCTAAAGGAAGAGGCTACTTTGTTTTCAAATTTGAGGTGATTTTTTTCTTTTACTTTTCAATCATTTTTTGTTTGAAACACTTTTTCTCTTTCAATGAAATGTTGTTATTTAGCAAAAAGTGCCAATTACATGAACTACTTATTATTTTCTTTTAGAAAAAAGTATATTTTTTCAGTAATCTATTCACTTTCTTTGTAAGTTTGTCTCTTCAAGGTTTGATGGGCTTGAATAAAAAATGTTTGCAAATTTTAATCTAGCTTGTGCTATTATTATTATTATATAATTTCGTTTCTTCTCCCAGAAAATGACATTGAATTCATCTCTCTTTGAAGCTATCAGTTCGTAACTTATAAAGTACTGTCTATATTTTGGTAGTTGTATGAAATGTAAAATATGAAAATACTTACAAAATGATTAAGGTAGCTGCACTCCATCATTTCCCTGTGAAAATAATAAAATCTGGATGGGCACACTGCTGTTCATTATGCTAAGATTTTGGAGCGCTTGAGCAGGACTATGATTGTACGGTGTCATTTGTGAGATCTCATTTCCTTGTGAGGGAGGGAGTTCGTATGAATGCACAAGGGAGTTCAAATCCTACATTGTCAATAGATCGGATTGACAAGACGTCCAACCGCTGGAAGAGGGCTGACATTCTCGTCTTCAACACCGGGCACTGGTGGGCTCATGGAAAAACTGCTCGAGGGTTAGGATACAATTATTTCATATTTCATATTTTGATTGTGTACATTCAATTCAGGAAACTGGATGTGCGAGTTTGTTTGTCTCCCCCATGTTTATTGAATTCTAACATGGTCAAAAAATAAAAAGGATTCATCTAAGTATTTGCTGCACTAGACTATTGACCTATATTTGTAACTTCTCTTGGCAACAACGAATGTTCGCACACACTATTCTATCAACCGATTATATGTATTAGGCAAGATTTGACACTGTTGTCTAACATGTTTATCTTGTTAAATTTTGGTTTTTTTCCCTTTCACAAATTAGTTTTTATTCCCTGCTATGTGGTTTGTCTTTCATTCCATAAAATTAACAAGTCGTCTGTCAAAAATAAACAATGGGAATAATTTTTATAATTATATTAAATACTTTGTATGATTTCTGCAGAATAAATTACTATAAAGAAGGTGATTATCTATATCCAAAATTTGATGGAGTTGAGGCATACAGAAAGGCTATAAAGACATGGGGAAAATGGATCGACAATAATATCAATCCAAAAAAGCAGATTGTCTACTATCGTGGATATTCTTCTGCTCATTTCAG
Protein sequence

>MS.gene57610.t1

MIFHNNRYTVATLFFLALFFLTIFFYSFFFFFNTTTTPLSPHTDLYLYIKPSLSPPPPPQQSSTTVTDIQPQPSIDGATVDVDDHESVDSVAVTNTDEVDDDAILALKECDLYNGTWVEDEDYPIYEPGSCPYVDEAYDCKINGRNDTHYTKWRWKPHGCDLPRFSAKDFLARLKGKQLMLVGDSMNRNQFESILCVLREGLDNKSKMYEIHGHTISKGRGYFVFKFEDYDCTVSFVRSHFLVREGVRMNAQGSSNPTLSIDRIDKTSNRWKRADILVFNTGHWWAHGKTARGINYYKEGDYLYPKFDGVEAYRKAIKTWGKWIDNNINPKKQIVYYRGYSSAHF