Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57612.t1 | XP_013458482.1 | 97.1 | 210 | 5 | 1 | 1 | 209 | 1 | 210 | 4.50E-111 | 410.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57612.t1 | Q5Z620 | 53.3 | 169 | 78 | 1 | 44 | 212 | 42 | 209 | 4.0e-44 | 179.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57612.t1 | A0A072UTW0 | 97.1 | 210 | 5 | 1 | 1 | 209 | 1 | 210 | 3.2e-111 | 410.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051862 | MS.gene57612 | 0.84205 | 3.28E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57612.t1 | MTR_4g126150 | 97.143 | 210 | 5 | 1 | 1 | 209 | 1 | 210 | 4.90e-146 | 417 |
| MS.gene57612.t1 | MTR_2g039410 | 67.111 | 225 | 56 | 5 | 1 | 212 | 1 | 220 | 3.15e-95 | 288 |
| MS.gene57612.t1 | MTR_4g126160 | 54.028 | 211 | 86 | 4 | 1 | 209 | 1 | 202 | 1.51e-66 | 213 |
| MS.gene57612.t1 | MTR_2g039340 | 61.176 | 170 | 61 | 2 | 44 | 209 | 37 | 205 | 6.70e-61 | 199 |
| MS.gene57612.t1 | MTR_7g090920 | 50.955 | 157 | 69 | 3 | 58 | 212 | 73 | 223 | 1.08e-44 | 156 |
| MS.gene57612.t1 | MTR_3g112350 | 46.569 | 204 | 96 | 7 | 12 | 212 | 15 | 208 | 6.17e-44 | 154 |
| MS.gene57612.t1 | MTR_4g044110 | 48.795 | 166 | 74 | 4 | 51 | 212 | 50 | 208 | 9.40e-44 | 153 |
| MS.gene57612.t1 | MTR_3g036100 | 48.193 | 166 | 74 | 4 | 51 | 212 | 74 | 231 | 3.34e-43 | 152 |
| MS.gene57612.t1 | MTR_0005s0070 | 50.685 | 146 | 61 | 4 | 71 | 212 | 1 | 139 | 1.05e-40 | 140 |
| MS.gene57612.t1 | MTR_1g015410 | 43.094 | 181 | 90 | 5 | 36 | 213 | 17 | 187 | 8.83e-37 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57612.t1 | AT5G56970 | 52.809 | 178 | 79 | 1 | 35 | 212 | 35 | 207 | 3.22e-54 | 179 |
| MS.gene57612.t1 | AT5G56970 | 52.809 | 178 | 79 | 1 | 35 | 212 | 35 | 207 | 3.67e-53 | 178 |
| MS.gene57612.t1 | AT1G75450 | 46.919 | 211 | 92 | 6 | 9 | 212 | 8 | 205 | 6.31e-50 | 170 |
| MS.gene57612.t1 | AT4G29740 | 46.479 | 213 | 95 | 6 | 11 | 212 | 9 | 213 | 3.14e-49 | 164 |
| MS.gene57612.t1 | AT1G75450 | 46.860 | 207 | 90 | 6 | 9 | 208 | 4 | 197 | 9.46e-49 | 167 |
| MS.gene57612.t1 | AT4G29740 | 46.479 | 213 | 95 | 6 | 11 | 212 | 9 | 213 | 1.26e-48 | 164 |
| MS.gene57612.t1 | AT4G29740 | 46.479 | 213 | 95 | 6 | 11 | 212 | 9 | 213 | 1.15e-47 | 164 |
| MS.gene57612.t1 | AT3G63440 | 51.592 | 157 | 70 | 2 | 58 | 212 | 60 | 212 | 7.36e-47 | 162 |
| MS.gene57612.t1 | AT2G41510 | 48.101 | 158 | 70 | 4 | 59 | 212 | 77 | 226 | 4.15e-43 | 150 |
| MS.gene57612.t1 | AT2G41510 | 48.101 | 158 | 70 | 4 | 59 | 212 | 77 | 226 | 1.53e-42 | 151 |
| MS.gene57612.t1 | AT2G41510 | 48.101 | 158 | 70 | 4 | 59 | 212 | 139 | 288 | 2.60e-42 | 151 |
| MS.gene57612.t1 | AT2G19500 | 42.927 | 205 | 92 | 3 | 13 | 212 | 6 | 190 | 6.80e-42 | 148 |
| MS.gene57612.t1 | AT5G21482 | 50.617 | 162 | 60 | 5 | 57 | 209 | 49 | 199 | 2.34e-38 | 139 |
Find 45 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGACTTGCACCTGTTTCT+TGG | 0.217615 | 8.4:+8856492 | MS.gene57612:CDS |
| AGTTATTAGTAGTATGAAAT+AGG | 0.276115 | 8.4:-8856030 | None:intergenic |
| GTTATGGAGTTTGGTTGATA+TGG | 0.310768 | 8.4:-8856132 | None:intergenic |
| TTTAGGGTCGTTATGGAGTT+TGG | 0.311295 | 8.4:-8856141 | None:intergenic |
| AAAATTGCTTCTAGTGACTA+TGG | 0.343388 | 8.4:+8856170 | MS.gene57612:CDS |
| AATCACCAACGTTCATGAAT+TGG | 0.351836 | 8.4:+8856595 | MS.gene57612:CDS |
| CTTTCCAATGCTGGAATTAG+TGG | 0.355413 | 8.4:+8856548 | MS.gene57612:CDS |
| CATGAATTGGATGTGATTAC+TGG | 0.369964 | 8.4:+8856608 | MS.gene57612:CDS |
| GTCTTTGAACACCCTTTGAT+TGG | 0.390776 | 8.4:+8856407 | MS.gene57612:CDS |
| ATTGGTTACTATGTTGATGT+TGG | 0.419823 | 8.4:+8856425 | MS.gene57612:CDS |
| AGTGGTCAGACGTTTCTTTA+CGG | 0.421013 | 8.4:+8856566 | MS.gene57612:CDS |
| ATGTTGGAGGAGAACAACTA+TGG | 0.426776 | 8.4:+8856441 | MS.gene57612:CDS |
| AGATAATCAGTCCAAGAAAC+AGG | 0.436827 | 8.4:-8856503 | None:intergenic |
| TCATGAACGTTGGTGATTTG+AGG | 0.440824 | 8.4:-8856590 | None:intergenic |
| TTCCTACGGTGGATATCAAA+CGG | 0.445255 | 8.4:-8856058 | None:intergenic |
| AGTCATGGCTTGTCCATTAG+TGG | 0.449428 | 8.4:-8856321 | None:intergenic |
| TCCTACGGTGGATATCAAAC+GGG | 0.453698 | 8.4:-8856057 | None:intergenic |
| GCTTCAGAAAACAAAGAAAA+GGG | 0.468438 | 8.4:+8856372 | MS.gene57612:CDS |
| ATTATGTCTTGTATAGAAGA+TGG | 0.477377 | 8.4:-8856230 | None:intergenic |
| GTGTTAACATTGGCTAGCTC+TGG | 0.478418 | 8.4:-8856110 | None:intergenic |
| AGCTTCAGAAAACAAAGAAA+AGG | 0.480651 | 8.4:+8856371 | MS.gene57612:CDS |
| GGGCAAGGTCACTCCACTAA+TGG | 0.480811 | 8.4:+8856308 | MS.gene57612:CDS |
| GGAGGAACACTTTCCAATGC+TGG | 0.491967 | 8.4:+8856539 | MS.gene57612:CDS |
| AGTGGAGTGACCTTGCCCTC+TGG | 0.499286 | 8.4:-8856303 | None:intergenic |
| TATCTTTACCTGACGGTTGG+AGG | 0.504841 | 8.4:+8856521 | MS.gene57612:CDS |
| GATTATCTTTACCTGACGGT+TGG | 0.521491 | 8.4:+8856518 | MS.gene57612:CDS |
| GGTTACTATGTTGATGTTGG+AGG | 0.524528 | 8.4:+8856428 | MS.gene57612:CDS |
| AAAGTGTTCCTCCAACCGTC+AGG | 0.525441 | 8.4:-8856529 | None:intergenic |
| CAACATAGTAACCAATCAAA+GGG | 0.525726 | 8.4:-8856418 | None:intergenic |
| GACTGATTATCTTTACCTGA+CGG | 0.526021 | 8.4:+8856514 | MS.gene57612:CDS |
| GGACAAGCCATGACTCATGA+TGG | 0.533340 | 8.4:+8856329 | MS.gene57612:CDS |
| TCAACATAGTAACCAATCAA+AGG | 0.543035 | 8.4:-8856419 | None:intergenic |
| GGTTGATATGGTGTTAACAT+TGG | 0.549802 | 8.4:-8856120 | None:intergenic |
| CTGACCACTAATTCCAGCAT+TGG | 0.549832 | 8.4:-8856552 | None:intergenic |
| TTAACATTGGCTAGCTCTGG+TGG | 0.549917 | 8.4:-8856107 | None:intergenic |
| ACTATAGCAGCCAGAGGGCA+AGG | 0.575490 | 8.4:+8856293 | MS.gene57612:CDS |
| CCTCTGGCTGCTATAGTGAA+AGG | 0.577125 | 8.4:-8856287 | None:intergenic |
| TACAATTCCATCATGAGTCA+TGG | 0.584996 | 8.4:-8856336 | None:intergenic |
| TTGTATGCAACTCTTGAACA+TGG | 0.595290 | 8.4:+8856473 | MS.gene57612:CDS |
| CACATCCAATTCATGAACGT+TGG | 0.600239 | 8.4:-8856600 | None:intergenic |
| CTTCAGAAAACAAAGAAAAG+GGG | 0.634602 | 8.4:+8856373 | MS.gene57612:CDS |
| CTTTCACTATAGCAGCCAGA+GGG | 0.652699 | 8.4:+8856288 | MS.gene57612:CDS |
| CCTTTCACTATAGCAGCCAG+AGG | 0.655121 | 8.4:+8856287 | MS.gene57612:CDS |
| TGATGGAATTGTAGTTGACA+TGG | 0.672980 | 8.4:+8856346 | MS.gene57612:CDS |
| ACCCGTTTGATATCCACCGT+AGG | 0.704257 | 8.4:+8856056 | MS.gene57612:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGTATGAGATTTTAATTA+AGG | - | chr8.4:8856258-8856277 | None:intergenic | 15.0% |
| !! | AGTTATTAGTAGTATGAAAT+AGG | - | chr8.4:8856033-8856052 | None:intergenic | 20.0% |
| ! | AGTATGAAATAGGTGAAAAA+AGG | - | chr8.4:8856023-8856042 | None:intergenic | 25.0% |
| ! | ATTATGTCTTGTATAGAAGA+TGG | - | chr8.4:8856233-8856252 | None:intergenic | 25.0% |
| ! | GTATGAAATAGGTGAAAAAA+GGG | - | chr8.4:8856022-8856041 | None:intergenic | 25.0% |
| !!! | AAGCAATTTTGATGGTTTTA+GGG | - | chr8.4:8856160-8856179 | None:intergenic | 25.0% |
| AAAATTGCTTCTAGTGACTA+TGG | + | chr8.4:8856170-8856189 | MS.gene57612:CDS | 30.0% | |
| AGCTTCAGAAAACAAAGAAA+AGG | + | chr8.4:8856371-8856390 | MS.gene57612:CDS | 30.0% | |
| ATGGATGAAAAACTGCTATT+GGG | - | chr8.4:8856214-8856233 | None:intergenic | 30.0% | |
| ATTGGTTACTATGTTGATGT+TGG | + | chr8.4:8856425-8856444 | MS.gene57612:CDS | 30.0% | |
| CAACATAGTAACCAATCAAA+GGG | - | chr8.4:8856421-8856440 | None:intergenic | 30.0% | |
| CTTCAGAAAACAAAGAAAAG+GGG | + | chr8.4:8856373-8856392 | MS.gene57612:CDS | 30.0% | |
| GCTTCAGAAAACAAAGAAAA+GGG | + | chr8.4:8856372-8856391 | MS.gene57612:CDS | 30.0% | |
| TCAACATAGTAACCAATCAA+AGG | - | chr8.4:8856422-8856441 | None:intergenic | 30.0% | |
| !! | GAAGCAATTTTGATGGTTTT+AGG | - | chr8.4:8856161-8856180 | None:intergenic | 30.0% |
| AATCACCAACGTTCATGAAT+TGG | + | chr8.4:8856595-8856614 | MS.gene57612:CDS | 35.0% | |
| AGATAATCAGTCCAAGAAAC+AGG | - | chr8.4:8856506-8856525 | None:intergenic | 35.0% | |
| CATGAATTGGATGTGATTAC+TGG | + | chr8.4:8856608-8856627 | MS.gene57612:CDS | 35.0% | |
| GACTGATTATCTTTACCTGA+CGG | + | chr8.4:8856514-8856533 | MS.gene57612:CDS | 35.0% | |
| GATGGATGAAAAACTGCTAT+TGG | - | chr8.4:8856215-8856234 | None:intergenic | 35.0% | |
| TACAATTCCATCATGAGTCA+TGG | - | chr8.4:8856339-8856358 | None:intergenic | 35.0% | |
| TGATGGAATTGTAGTTGACA+TGG | + | chr8.4:8856346-8856365 | MS.gene57612:CDS | 35.0% | |
| TTGTATGCAACTCTTGAACA+TGG | + | chr8.4:8856473-8856492 | MS.gene57612:CDS | 35.0% | |
| ! | GGTTGATATGGTGTTAACAT+TGG | - | chr8.4:8856123-8856142 | None:intergenic | 35.0% |
| ! | GTCACTAGAAGCAATTTTGA+TGG | - | chr8.4:8856168-8856187 | None:intergenic | 35.0% |
| ! | GTTATGGAGTTTGGTTGATA+TGG | - | chr8.4:8856135-8856154 | None:intergenic | 35.0% |
| AGTGGTCAGACGTTTCTTTA+CGG | + | chr8.4:8856566-8856585 | MS.gene57612:CDS | 40.0% | |
| ATGTTGGAGGAGAACAACTA+TGG | + | chr8.4:8856441-8856460 | MS.gene57612:CDS | 40.0% | |
| CACATCCAATTCATGAACGT+TGG | - | chr8.4:8856603-8856622 | None:intergenic | 40.0% | |
| CTTTCCAATGCTGGAATTAG+TGG | + | chr8.4:8856548-8856567 | MS.gene57612:CDS | 40.0% | |
| GATTATCTTTACCTGACGGT+TGG | + | chr8.4:8856518-8856537 | MS.gene57612:CDS | 40.0% | |
| GGTTACTATGTTGATGTTGG+AGG | + | chr8.4:8856428-8856447 | MS.gene57612:CDS | 40.0% | |
| GTCTTTGAACACCCTTTGAT+TGG | + | chr8.4:8856407-8856426 | MS.gene57612:CDS | 40.0% | |
| TTCCTACGGTGGATATCAAA+CGG | - | chr8.4:8856061-8856080 | None:intergenic | 40.0% | |
| ! | TTTAGGGTCGTTATGGAGTT+TGG | - | chr8.4:8856144-8856163 | None:intergenic | 40.0% |
| !! | TAGCAGAGTTTTGCACTGAT+CGG | - | chr8.4:8856087-8856106 | None:intergenic | 40.0% |
| !! | TCATGAACGTTGGTGATTTG+AGG | - | chr8.4:8856593-8856612 | None:intergenic | 40.0% |
| !!! | TGATGGTTTTAGGGTCGTTA+TGG | - | chr8.4:8856151-8856170 | None:intergenic | 40.0% |
| CTGACCACTAATTCCAGCAT+TGG | - | chr8.4:8856555-8856574 | None:intergenic | 45.0% | |
| CTTTCACTATAGCAGCCAGA+GGG | + | chr8.4:8856288-8856307 | MS.gene57612:CDS | 45.0% | |
| TATCTTTACCTGACGGTTGG+AGG | + | chr8.4:8856521-8856540 | MS.gene57612:CDS | 45.0% | |
| TCCTACGGTGGATATCAAAC+GGG | - | chr8.4:8856060-8856079 | None:intergenic | 45.0% | |
| ! | AGTCATGGCTTGTCCATTAG+TGG | - | chr8.4:8856324-8856343 | None:intergenic | 45.0% |
| ! | ATGGACTTGCACCTGTTTCT+TGG | + | chr8.4:8856492-8856511 | MS.gene57612:CDS | 45.0% |
| ! | GTGTTAACATTGGCTAGCTC+TGG | - | chr8.4:8856113-8856132 | None:intergenic | 45.0% |
| !! | GCACTGATCGGTTTTTCCTA+CGG | - | chr8.4:8856075-8856094 | None:intergenic | 45.0% |
| !! | TTAACATTGGCTAGCTCTGG+TGG | - | chr8.4:8856110-8856129 | None:intergenic | 45.0% |
| ACCCGTTTGATATCCACCGT+AGG | + | chr8.4:8856056-8856075 | MS.gene57612:CDS | 50.0% | |
| CCTCTGGCTGCTATAGTGAA+AGG | - | chr8.4:8856290-8856309 | None:intergenic | 50.0% | |
| CCTTTCACTATAGCAGCCAG+AGG | + | chr8.4:8856287-8856306 | MS.gene57612:CDS | 50.0% | |
| GGACAAGCCATGACTCATGA+TGG | + | chr8.4:8856329-8856348 | MS.gene57612:CDS | 50.0% | |
| GGAGGAACACTTTCCAATGC+TGG | + | chr8.4:8856539-8856558 | MS.gene57612:CDS | 50.0% | |
| ! | AAAGTGTTCCTCCAACCGTC+AGG | - | chr8.4:8856532-8856551 | None:intergenic | 50.0% |
| !! | CTGATCGGTTTTTCCTACGG+TGG | - | chr8.4:8856072-8856091 | None:intergenic | 50.0% |
| ACTATAGCAGCCAGAGGGCA+AGG | + | chr8.4:8856293-8856312 | MS.gene57612:CDS | 55.0% | |
| GGGCAAGGTCACTCCACTAA+TGG | + | chr8.4:8856308-8856327 | MS.gene57612:CDS | 55.0% | |
| AGTGGAGTGACCTTGCCCTC+TGG | - | chr8.4:8856306-8856325 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 8856005 | 8856652 | 8856005 | ID=MS.gene57612 |
| chr8.4 | mRNA | 8856005 | 8856652 | 8856005 | ID=MS.gene57612.t1;Parent=MS.gene57612 |
| chr8.4 | exon | 8856005 | 8856652 | 8856005 | ID=MS.gene57612.t1.exon1;Parent=MS.gene57612.t1 |
| chr8.4 | CDS | 8856005 | 8856652 | 8856005 | ID=cds.MS.gene57612.t1;Parent=MS.gene57612.t1 |
| Gene Sequence |
| Protein sequence |
