Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57614.t1 | XP_013458482.1 | 97.1 | 210 | 5 | 1 | 1 | 209 | 1 | 210 | 4.50E-111 | 410.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57614.t1 | Q5Z620 | 53.3 | 169 | 78 | 1 | 44 | 212 | 42 | 209 | 4.0e-44 | 179.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57614.t1 | A0A072UTW0 | 97.1 | 210 | 5 | 1 | 1 | 209 | 1 | 210 | 3.2e-111 | 410.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051862 | MS.gene57614 | 0.847528 | 1.09E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57614.t1 | MTR_4g126150 | 97.143 | 210 | 5 | 1 | 1 | 209 | 1 | 210 | 4.90e-146 | 417 |
| MS.gene57614.t1 | MTR_2g039410 | 67.111 | 225 | 56 | 5 | 1 | 212 | 1 | 220 | 3.15e-95 | 288 |
| MS.gene57614.t1 | MTR_4g126160 | 54.028 | 211 | 86 | 4 | 1 | 209 | 1 | 202 | 1.51e-66 | 213 |
| MS.gene57614.t1 | MTR_2g039340 | 61.176 | 170 | 61 | 2 | 44 | 209 | 37 | 205 | 6.70e-61 | 199 |
| MS.gene57614.t1 | MTR_7g090920 | 50.955 | 157 | 69 | 3 | 58 | 212 | 73 | 223 | 1.08e-44 | 156 |
| MS.gene57614.t1 | MTR_3g112350 | 46.569 | 204 | 96 | 7 | 12 | 212 | 15 | 208 | 6.17e-44 | 154 |
| MS.gene57614.t1 | MTR_4g044110 | 48.795 | 166 | 74 | 4 | 51 | 212 | 50 | 208 | 9.40e-44 | 153 |
| MS.gene57614.t1 | MTR_3g036100 | 48.193 | 166 | 74 | 4 | 51 | 212 | 74 | 231 | 3.34e-43 | 152 |
| MS.gene57614.t1 | MTR_0005s0070 | 50.685 | 146 | 61 | 4 | 71 | 212 | 1 | 139 | 1.05e-40 | 140 |
| MS.gene57614.t1 | MTR_1g015410 | 43.094 | 181 | 90 | 5 | 36 | 213 | 17 | 187 | 8.83e-37 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57614.t1 | AT5G56970 | 52.809 | 178 | 79 | 1 | 35 | 212 | 35 | 207 | 3.22e-54 | 179 |
| MS.gene57614.t1 | AT5G56970 | 52.809 | 178 | 79 | 1 | 35 | 212 | 35 | 207 | 3.67e-53 | 178 |
| MS.gene57614.t1 | AT1G75450 | 46.919 | 211 | 92 | 6 | 9 | 212 | 8 | 205 | 6.31e-50 | 170 |
| MS.gene57614.t1 | AT4G29740 | 46.479 | 213 | 95 | 6 | 11 | 212 | 9 | 213 | 3.14e-49 | 164 |
| MS.gene57614.t1 | AT1G75450 | 46.860 | 207 | 90 | 6 | 9 | 208 | 4 | 197 | 9.46e-49 | 167 |
| MS.gene57614.t1 | AT4G29740 | 46.479 | 213 | 95 | 6 | 11 | 212 | 9 | 213 | 1.26e-48 | 164 |
| MS.gene57614.t1 | AT4G29740 | 46.479 | 213 | 95 | 6 | 11 | 212 | 9 | 213 | 1.15e-47 | 164 |
| MS.gene57614.t1 | AT3G63440 | 51.592 | 157 | 70 | 2 | 58 | 212 | 60 | 212 | 7.36e-47 | 162 |
| MS.gene57614.t1 | AT2G41510 | 48.101 | 158 | 70 | 4 | 59 | 212 | 77 | 226 | 4.15e-43 | 150 |
| MS.gene57614.t1 | AT2G41510 | 48.101 | 158 | 70 | 4 | 59 | 212 | 77 | 226 | 1.53e-42 | 151 |
| MS.gene57614.t1 | AT2G41510 | 48.101 | 158 | 70 | 4 | 59 | 212 | 139 | 288 | 2.60e-42 | 151 |
| MS.gene57614.t1 | AT2G19500 | 42.927 | 205 | 92 | 3 | 13 | 212 | 6 | 190 | 6.80e-42 | 148 |
| MS.gene57614.t1 | AT5G21482 | 50.617 | 162 | 60 | 5 | 57 | 209 | 49 | 199 | 2.34e-38 | 139 |
Find 45 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGACTTGCACCTGTTTCT+TGG | 0.217615 | 8.3:+4411843 | MS.gene57614:CDS |
| AGTTATTAGTAGTATGAAAT+AGG | 0.276115 | 8.3:-4411381 | None:intergenic |
| GTTATGGAGTTTGGTTGATA+TGG | 0.310768 | 8.3:-4411483 | None:intergenic |
| TTTAGGGTCGTTATGGAGTT+TGG | 0.311295 | 8.3:-4411492 | None:intergenic |
| AAAATTGCTTCTAGTGACTA+TGG | 0.343388 | 8.3:+4411521 | MS.gene57614:CDS |
| AATCACCAACGTTCATGAAT+TGG | 0.351836 | 8.3:+4411946 | MS.gene57614:CDS |
| CTTTCCAATGCTGGAATTAG+TGG | 0.355413 | 8.3:+4411899 | MS.gene57614:CDS |
| CATGAATTGGATGTGATTAC+TGG | 0.369964 | 8.3:+4411959 | MS.gene57614:CDS |
| GTCTTTGAACACCCTTTGAT+TGG | 0.390776 | 8.3:+4411758 | MS.gene57614:CDS |
| ATTGGTTACTATGTTGATGT+TGG | 0.419823 | 8.3:+4411776 | MS.gene57614:CDS |
| AGTGGTCAGACGTTTCTTTA+CGG | 0.421013 | 8.3:+4411917 | MS.gene57614:CDS |
| ATGTTGGAGGAGAACAACTA+TGG | 0.426776 | 8.3:+4411792 | MS.gene57614:CDS |
| AGATAATCAGTCCAAGAAAC+AGG | 0.436827 | 8.3:-4411854 | None:intergenic |
| TCATGAACGTTGGTGATTTG+AGG | 0.440824 | 8.3:-4411941 | None:intergenic |
| TTCCTACGGTGGATATCAAA+CGG | 0.445255 | 8.3:-4411409 | None:intergenic |
| AGTCATGGCTTGTCCATTAG+TGG | 0.449428 | 8.3:-4411672 | None:intergenic |
| TCCTACGGTGGATATCAAAC+GGG | 0.453698 | 8.3:-4411408 | None:intergenic |
| GCTTCAGAAAACAAAGAAAA+GGG | 0.468438 | 8.3:+4411723 | MS.gene57614:CDS |
| ATTATGTCTTGTATAGAAGA+TGG | 0.477377 | 8.3:-4411581 | None:intergenic |
| GTGTTAACATTGGCTAGCTC+TGG | 0.478418 | 8.3:-4411461 | None:intergenic |
| AGCTTCAGAAAACAAAGAAA+AGG | 0.480651 | 8.3:+4411722 | MS.gene57614:CDS |
| GGGCAAGGTCACTCCACTAA+TGG | 0.480811 | 8.3:+4411659 | MS.gene57614:CDS |
| GGAGGAACACTTTCCAATGC+TGG | 0.491967 | 8.3:+4411890 | MS.gene57614:CDS |
| AGTGGAGTGACCTTGCCCTC+TGG | 0.499286 | 8.3:-4411654 | None:intergenic |
| TATCTTTACCTGACGGTTGG+AGG | 0.504841 | 8.3:+4411872 | MS.gene57614:CDS |
| GATTATCTTTACCTGACGGT+TGG | 0.521491 | 8.3:+4411869 | MS.gene57614:CDS |
| GGTTACTATGTTGATGTTGG+AGG | 0.524528 | 8.3:+4411779 | MS.gene57614:CDS |
| AAAGTGTTCCTCCAACCGTC+AGG | 0.525441 | 8.3:-4411880 | None:intergenic |
| CAACATAGTAACCAATCAAA+GGG | 0.525726 | 8.3:-4411769 | None:intergenic |
| GACTGATTATCTTTACCTGA+CGG | 0.526021 | 8.3:+4411865 | MS.gene57614:CDS |
| GGACAAGCCATGACTCATGA+TGG | 0.533340 | 8.3:+4411680 | MS.gene57614:CDS |
| TCAACATAGTAACCAATCAA+AGG | 0.543035 | 8.3:-4411770 | None:intergenic |
| GGTTGATATGGTGTTAACAT+TGG | 0.549802 | 8.3:-4411471 | None:intergenic |
| CTGACCACTAATTCCAGCAT+TGG | 0.549832 | 8.3:-4411903 | None:intergenic |
| TTAACATTGGCTAGCTCTGG+TGG | 0.549917 | 8.3:-4411458 | None:intergenic |
| ACTATAGCAGCCAGAGGGCA+AGG | 0.575490 | 8.3:+4411644 | MS.gene57614:CDS |
| CCTCTGGCTGCTATAGTGAA+AGG | 0.577125 | 8.3:-4411638 | None:intergenic |
| TACAATTCCATCATGAGTCA+TGG | 0.584996 | 8.3:-4411687 | None:intergenic |
| TTGTATGCAACTCTTGAACA+TGG | 0.595290 | 8.3:+4411824 | MS.gene57614:CDS |
| CACATCCAATTCATGAACGT+TGG | 0.600239 | 8.3:-4411951 | None:intergenic |
| CTTCAGAAAACAAAGAAAAG+GGG | 0.634602 | 8.3:+4411724 | MS.gene57614:CDS |
| CTTTCACTATAGCAGCCAGA+GGG | 0.652699 | 8.3:+4411639 | MS.gene57614:CDS |
| CCTTTCACTATAGCAGCCAG+AGG | 0.655121 | 8.3:+4411638 | MS.gene57614:CDS |
| TGATGGAATTGTAGTTGACA+TGG | 0.672980 | 8.3:+4411697 | MS.gene57614:CDS |
| ACCCGTTTGATATCCACCGT+AGG | 0.704257 | 8.3:+4411407 | MS.gene57614:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGTATGAGATTTTAATTA+AGG | - | chr8.3:4411609-4411628 | None:intergenic | 15.0% |
| !! | AGTTATTAGTAGTATGAAAT+AGG | - | chr8.3:4411384-4411403 | None:intergenic | 20.0% |
| ! | AGTATGAAATAGGTGAAAAA+AGG | - | chr8.3:4411374-4411393 | None:intergenic | 25.0% |
| ! | ATTATGTCTTGTATAGAAGA+TGG | - | chr8.3:4411584-4411603 | None:intergenic | 25.0% |
| ! | GTATGAAATAGGTGAAAAAA+GGG | - | chr8.3:4411373-4411392 | None:intergenic | 25.0% |
| !!! | AAGCAATTTTGATGGTTTTA+GGG | - | chr8.3:4411511-4411530 | None:intergenic | 25.0% |
| AAAATTGCTTCTAGTGACTA+TGG | + | chr8.3:4411521-4411540 | MS.gene57614:CDS | 30.0% | |
| AGCTTCAGAAAACAAAGAAA+AGG | + | chr8.3:4411722-4411741 | MS.gene57614:CDS | 30.0% | |
| ATGGATGAAAAACTGCTATT+GGG | - | chr8.3:4411565-4411584 | None:intergenic | 30.0% | |
| ATTGGTTACTATGTTGATGT+TGG | + | chr8.3:4411776-4411795 | MS.gene57614:CDS | 30.0% | |
| CAACATAGTAACCAATCAAA+GGG | - | chr8.3:4411772-4411791 | None:intergenic | 30.0% | |
| CTTCAGAAAACAAAGAAAAG+GGG | + | chr8.3:4411724-4411743 | MS.gene57614:CDS | 30.0% | |
| GCTTCAGAAAACAAAGAAAA+GGG | + | chr8.3:4411723-4411742 | MS.gene57614:CDS | 30.0% | |
| TCAACATAGTAACCAATCAA+AGG | - | chr8.3:4411773-4411792 | None:intergenic | 30.0% | |
| !! | GAAGCAATTTTGATGGTTTT+AGG | - | chr8.3:4411512-4411531 | None:intergenic | 30.0% |
| AATCACCAACGTTCATGAAT+TGG | + | chr8.3:4411946-4411965 | MS.gene57614:CDS | 35.0% | |
| AGATAATCAGTCCAAGAAAC+AGG | - | chr8.3:4411857-4411876 | None:intergenic | 35.0% | |
| CATGAATTGGATGTGATTAC+TGG | + | chr8.3:4411959-4411978 | MS.gene57614:CDS | 35.0% | |
| GACTGATTATCTTTACCTGA+CGG | + | chr8.3:4411865-4411884 | MS.gene57614:CDS | 35.0% | |
| GATGGATGAAAAACTGCTAT+TGG | - | chr8.3:4411566-4411585 | None:intergenic | 35.0% | |
| TACAATTCCATCATGAGTCA+TGG | - | chr8.3:4411690-4411709 | None:intergenic | 35.0% | |
| TGATGGAATTGTAGTTGACA+TGG | + | chr8.3:4411697-4411716 | MS.gene57614:CDS | 35.0% | |
| TTGTATGCAACTCTTGAACA+TGG | + | chr8.3:4411824-4411843 | MS.gene57614:CDS | 35.0% | |
| ! | GGTTGATATGGTGTTAACAT+TGG | - | chr8.3:4411474-4411493 | None:intergenic | 35.0% |
| ! | GTCACTAGAAGCAATTTTGA+TGG | - | chr8.3:4411519-4411538 | None:intergenic | 35.0% |
| ! | GTTATGGAGTTTGGTTGATA+TGG | - | chr8.3:4411486-4411505 | None:intergenic | 35.0% |
| AGTGGTCAGACGTTTCTTTA+CGG | + | chr8.3:4411917-4411936 | MS.gene57614:CDS | 40.0% | |
| ATGTTGGAGGAGAACAACTA+TGG | + | chr8.3:4411792-4411811 | MS.gene57614:CDS | 40.0% | |
| CACATCCAATTCATGAACGT+TGG | - | chr8.3:4411954-4411973 | None:intergenic | 40.0% | |
| CTTTCCAATGCTGGAATTAG+TGG | + | chr8.3:4411899-4411918 | MS.gene57614:CDS | 40.0% | |
| GATTATCTTTACCTGACGGT+TGG | + | chr8.3:4411869-4411888 | MS.gene57614:CDS | 40.0% | |
| GGTTACTATGTTGATGTTGG+AGG | + | chr8.3:4411779-4411798 | MS.gene57614:CDS | 40.0% | |
| GTCTTTGAACACCCTTTGAT+TGG | + | chr8.3:4411758-4411777 | MS.gene57614:CDS | 40.0% | |
| TTCCTACGGTGGATATCAAA+CGG | - | chr8.3:4411412-4411431 | None:intergenic | 40.0% | |
| ! | TTTAGGGTCGTTATGGAGTT+TGG | - | chr8.3:4411495-4411514 | None:intergenic | 40.0% |
| !! | TAGCAGAGTTTTGCACTGAT+CGG | - | chr8.3:4411438-4411457 | None:intergenic | 40.0% |
| !! | TCATGAACGTTGGTGATTTG+AGG | - | chr8.3:4411944-4411963 | None:intergenic | 40.0% |
| !!! | TGATGGTTTTAGGGTCGTTA+TGG | - | chr8.3:4411502-4411521 | None:intergenic | 40.0% |
| CTGACCACTAATTCCAGCAT+TGG | - | chr8.3:4411906-4411925 | None:intergenic | 45.0% | |
| CTTTCACTATAGCAGCCAGA+GGG | + | chr8.3:4411639-4411658 | MS.gene57614:CDS | 45.0% | |
| TATCTTTACCTGACGGTTGG+AGG | + | chr8.3:4411872-4411891 | MS.gene57614:CDS | 45.0% | |
| TCCTACGGTGGATATCAAAC+GGG | - | chr8.3:4411411-4411430 | None:intergenic | 45.0% | |
| ! | AGTCATGGCTTGTCCATTAG+TGG | - | chr8.3:4411675-4411694 | None:intergenic | 45.0% |
| ! | ATGGACTTGCACCTGTTTCT+TGG | + | chr8.3:4411843-4411862 | MS.gene57614:CDS | 45.0% |
| ! | GTGTTAACATTGGCTAGCTC+TGG | - | chr8.3:4411464-4411483 | None:intergenic | 45.0% |
| !! | GCACTGATCGGTTTTTCCTA+CGG | - | chr8.3:4411426-4411445 | None:intergenic | 45.0% |
| !! | TTAACATTGGCTAGCTCTGG+TGG | - | chr8.3:4411461-4411480 | None:intergenic | 45.0% |
| ACCCGTTTGATATCCACCGT+AGG | + | chr8.3:4411407-4411426 | MS.gene57614:CDS | 50.0% | |
| CCTCTGGCTGCTATAGTGAA+AGG | - | chr8.3:4411641-4411660 | None:intergenic | 50.0% | |
| CCTTTCACTATAGCAGCCAG+AGG | + | chr8.3:4411638-4411657 | MS.gene57614:CDS | 50.0% | |
| GGACAAGCCATGACTCATGA+TGG | + | chr8.3:4411680-4411699 | MS.gene57614:CDS | 50.0% | |
| GGAGGAACACTTTCCAATGC+TGG | + | chr8.3:4411890-4411909 | MS.gene57614:CDS | 50.0% | |
| ! | AAAGTGTTCCTCCAACCGTC+AGG | - | chr8.3:4411883-4411902 | None:intergenic | 50.0% |
| !! | CTGATCGGTTTTTCCTACGG+TGG | - | chr8.3:4411423-4411442 | None:intergenic | 50.0% |
| ACTATAGCAGCCAGAGGGCA+AGG | + | chr8.3:4411644-4411663 | MS.gene57614:CDS | 55.0% | |
| GGGCAAGGTCACTCCACTAA+TGG | + | chr8.3:4411659-4411678 | MS.gene57614:CDS | 55.0% | |
| AGTGGAGTGACCTTGCCCTC+TGG | - | chr8.3:4411657-4411676 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 4411356 | 4412003 | 4411356 | ID=MS.gene57614 |
| chr8.3 | mRNA | 4411356 | 4412003 | 4411356 | ID=MS.gene57614.t1;Parent=MS.gene57614 |
| chr8.3 | exon | 4411356 | 4412003 | 4411356 | ID=MS.gene57614.t1.exon1;Parent=MS.gene57614.t1 |
| chr8.3 | CDS | 4411356 | 4412003 | 4411356 | ID=cds.MS.gene57614.t1;Parent=MS.gene57614.t1 |
| Gene Sequence |
| Protein sequence |
