Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57753.t1 | XP_013458135.1 | 100 | 81 | 0 | 0 | 1 | 81 | 1544 | 1624 | 2.50E-41 | 177.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57753.t1 | P84634 | 53.8 | 78 | 36 | 0 | 1 | 78 | 1624 | 1701 | 2.3e-19 | 95.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57753.t1 | A0A072US37 | 100.0 | 81 | 0 | 0 | 1 | 81 | 1544 | 1624 | 1.8e-41 | 177.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052611 | MS.gene57753 | 0.808365 | 3.21E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAACAAAAAAGATATTGA+GGG | + | 32401:6067-6086 | MS.gene57753:intergenic | 15.0% |
| !! | TTAAAACAAAAAAGATATTG+AGG | + | 32401:6068-6087 | MS.gene57753:intergenic | 15.0% |
| !! | AATATGATTATCGAGTTTAT+TGG | - | 32401:6491-6510 | MS.gene57753:intron | 20.0% |
| !! | ATATGATTATCGAGTTTATT+GGG | - | 32401:6492-6511 | MS.gene57753:intron | 20.0% |
| !!! | TTTCCTTTTCTAGTAAATAT+AGG | + | 32401:6231-6250 | MS.gene57753:intergenic | 20.0% |
| ! | AAACTCGATAATCATATTTG+AGG | + | 32401:6489-6508 | MS.gene57753:intergenic | 25.0% |
| ! | ACAGAGAATTTCACAAAAAA+TGG | + | 32401:6126-6145 | MS.gene57753:intergenic | 25.0% |
| ! | ACTCCTATATTTACTAGAAA+AGG | - | 32401:6225-6244 | MS.gene57753:intron | 25.0% |
| ! | CAGAGAATTTCACAAAAAAT+GGG | + | 32401:6125-6144 | MS.gene57753:intergenic | 25.0% |
| ! | TAGGGAATACAATCAAAATA+AGG | - | 32401:6192-6211 | MS.gene57753:intron | 25.0% |
| ! | TATGATTATCGAGTTTATTG+GGG | - | 32401:6493-6512 | MS.gene57753:intron | 25.0% |
| ! | TGTGCAACATAAAAAACTTT+CGG | - | 32401:6339-6358 | MS.gene57753:intron | 25.0% |
| !!! | TTATTTTGATTGTATTCCCT+AGG | + | 32401:6193-6212 | MS.gene57753:intergenic | 25.0% |
| ATGCGATACAAAAATACATG+AGG | - | 32401:6157-6176 | MS.gene57753:intron | 30.0% | |
| TCGCTTGGTATATTAACTTT+CGG | - | 32401:6368-6387 | MS.gene57753:intron | 30.0% | |
| TGGTTGAAGTAAAAGAGTTA+AGG | + | 32401:6272-6291 | MS.gene57753:intergenic | 30.0% | |
| ! | AATTATGTGCTGCTAATTAC+TGG | - | 32401:5982-6001 | MS.gene57753:CDS | 30.0% |
| ! | GTGAATCTAAAATGAAGCAA+AGG | + | 32401:6437-6456 | MS.gene57753:intergenic | 30.0% |
| ! | TCATTTTAGATTCACTTGCA+TGG | - | 32401:6442-6461 | MS.gene57753:intron | 30.0% |
| ATGAGGTTGTAAATAGCCTA+GGG | - | 32401:6174-6193 | MS.gene57753:intron | 35.0% | |
| CTTGGTATATTAACTTTCGG+TGG | - | 32401:6371-6390 | MS.gene57753:intron | 35.0% | |
| TAGGAGCTATAAAGTTGTCA+TGG | + | 32401:6295-6314 | MS.gene57753:intergenic | 35.0% | |
| ! | TTTTGGTACCTGAAAGAGAA+AGG | - | 32401:6566-6585 | MS.gene57753:CDS | 35.0% |
| ACTGTCCATGCATCAACAAT+AGG | + | 32401:6314-6333 | MS.gene57752:intergenic | 40.0% | |
| AGGTTACCTGCATAATGCTA+AGG | - | 32401:6586-6605 | MS.gene57753:CDS | 40.0% | |
| AGTTACCCTTAGCATTATGC+AGG | + | 32401:6595-6614 | MS.gene57753:intergenic | 40.0% | |
| ATTCACTTGCATGGTGACAA+TGG | - | 32401:6451-6470 | MS.gene57753:intron | 40.0% | |
| ATTGAGGGACTCACAACTTA+AGG | + | 32401:6052-6071 | MS.gene57753:intergenic | 40.0% | |
| CATGAGGTTGTAAATAGCCT+AGG | - | 32401:6173-6192 | MS.gene57753:intron | 40.0% | |
| GAATCTTTCTTCTTTGGCAG+AGG | + | 32401:6521-6540 | MS.gene57753:intergenic | 40.0% | |
| GAGCTATAAAGTTGTCATGG+TGG | + | 32401:6292-6311 | MS.gene57753:intergenic | 40.0% | |
| GCCAAAGAAGAAAGATTCAG+CGG | - | 32401:6523-6542 | MS.gene57753:intron | 40.0% | |
| GGTTACCTGCATAATGCTAA+GGG | - | 32401:6587-6606 | MS.gene57753:CDS | 40.0% | |
| TAGCTCCTATTGTTGATGCA+TGG | - | 32401:6306-6325 | MS.gene57753:intron | 40.0% | |
| TCCGCTGAATCTTTCTTCTT+TGG | + | 32401:6527-6546 | MS.gene57753:intergenic | 40.0% | |
| TGTTGTGCAACACGATATCA+AGG | - | 32401:6396-6415 | MS.gene57753:intron | 40.0% | |
| !! | AACTTTCGGTGTTTATCGCT+TGG | - | 32401:6353-6372 | MS.gene57753:intron | 40.0% |
| ACTCACAACTTAAGGTGGTC+TGG | + | 32401:6044-6063 | MS.gene57752:intergenic | 45.0% | |
| ATTTGACTGCTGCAAGGAAG+AGG | - | 32401:6016-6035 | MS.gene57753:CDS | 45.0% | |
| GCAGGTAACCTTTCTCTTTC+AGG | + | 32401:6577-6596 | MS.gene57753:intergenic | 45.0% | |
| GGTGACAATGGAGATAGAAG+AGG | - | 32401:6463-6482 | MS.gene57753:intron | 45.0% | |
| TCCTTCATTTGACTGCTGCA+AGG | - | 32401:6010-6029 | MS.gene57753:CDS | 45.0% | |
| TCCTTGCAGCAGTCAAATGA+AGG | + | 32401:6014-6033 | MS.gene57753:intergenic | 45.0% | |
| TTGCAGCAGTCAAATGAAGG+AGG | + | 32401:6011-6030 | MS.gene57753:intergenic | 45.0% | |
| TTTGACTGCTGCAAGGAAGA+GGG | - | 32401:6017-6036 | MS.gene57753:CDS | 45.0% | |
| GAGGGACTCACAACTTAAGG+TGG | + | 32401:6049-6068 | MS.gene57753:intergenic | 50.0% | |
| TTCAGCGGATAGTGCAGCTG+AGG | - | 32401:6538-6557 | MS.gene57753:CDS | 55.0% | |
| ! | GTGCAGCTGAGGCTGCATTT+TGG | - | 32401:6549-6568 | MS.gene57753:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32401 | gene | 5968 | 6616 | 5968 | ID=MS.gene57753 |
| 32401 | mRNA | 5968 | 6616 | 5968 | ID=MS.gene57753.t1;Parent=MS.gene57753 |
| 32401 | exon | 6527 | 6616 | 6527 | ID=MS.gene57753.t1.exon1;Parent=MS.gene57753.t1 |
| 32401 | CDS | 6527 | 6616 | 6527 | ID=cds.MS.gene57753.t1;Parent=MS.gene57753.t1 |
| 32401 | exon | 5968 | 6133 | 5968 | ID=MS.gene57753.t1.exon2;Parent=MS.gene57753.t1 |
| 32401 | CDS | 5968 | 6133 | 5968 | ID=cds.MS.gene57753.t1;Parent=MS.gene57753.t1 |
| Gene Sequence |
| Protein sequence |
