Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57993.t1 | XP_003609475.2 | 94.4 | 54 | 3 | 0 | 52 | 105 | 37 | 90 | 4.50E-16 | 94 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57993.t1 | G7JGL2 | 94.4 | 54 | 3 | 0 | 52 | 105 | 37 | 90 | 3.3e-16 | 94.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055957 | MS.gene57993 | 0.801213 | 1.00E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57993.t1 | MTR_4g116080 | 92.647 | 68 | 5 | 0 | 38 | 105 | 23 | 90 | 5.52e-35 | 119 |
| MS.gene57993.t1 | MTR_7g070110 | 87.500 | 32 | 4 | 0 | 1 | 32 | 86 | 117 | 2.93e-14 | 63.9 |
| MS.gene57993.t1 | MTR_7g039300 | 76.471 | 34 | 8 | 0 | 1 | 34 | 133 | 166 | 8.57e-13 | 62.4 |
| MS.gene57993.t1 | MTR_7g045450 | 76.471 | 34 | 8 | 0 | 1 | 34 | 102 | 135 | 9.94e-13 | 62.4 |
| MS.gene57993.t1 | MTR_6g073570 | 76.471 | 34 | 8 | 0 | 1 | 34 | 102 | 135 | 9.94e-13 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGTTTCATCTCTCGCCTT+TGG | 0.343143 | 8.3:+10313922 | MS.gene57993:CDS |
| GAGGAGGTAAGCGAGGGATT+TGG | 0.392521 | 8.3:-10314013 | None:intergenic |
| AGAGATCAAAATGTCTTTGA+AGG | 0.393026 | 8.3:-10313029 | None:intergenic |
| GATCGAGTTGTTGTTTCCAA+AGG | 0.517156 | 8.3:-10313938 | None:intergenic |
| GGGAAATGCGAGATTTACTA+AGG | 0.553921 | 8.3:+10312964 | MS.gene57993:CDS |
| CAATCATTTGATATCACCAA+TGG | 0.555785 | 8.3:+10314957 | MS.gene57993:CDS |
| TGATATCACCAATGGATGGA+AGG | 0.586037 | 8.3:+10314965 | MS.gene57993:CDS |
| CGCTATGAGGAGGTAAGCGA+GGG | 0.590126 | 8.3:-10314019 | None:intergenic |
| ATGAATTCAAACACGTTCCG+AGG | 0.597619 | 8.3:-10313004 | None:intergenic |
| CATTTGATATCACCAATGGA+TGG | 0.597818 | 8.3:+10314961 | MS.gene57993:CDS |
| ATAGGAAGTATACCGCTATG+AGG | 0.615472 | 8.3:-10314032 | None:intergenic |
| TGAATGCAAACAATGTGCCT+CGG | 0.616425 | 8.3:+10312987 | MS.gene57993:CDS |
| CCGCTATGAGGAGGTAAGCG+AGG | 0.617664 | 8.3:-10314020 | None:intergenic |
| AGTACTCACAGCGGCAACAA+TGG | 0.635632 | 8.3:+10313900 | MS.gene57993:CDS |
| GATCTCTCAGGAAGATGACG+TGG | 0.658562 | 8.3:+10313045 | MS.gene57993:CDS |
| GGAAGTATACCGCTATGAGG+AGG | 0.668264 | 8.3:-10314029 | None:intergenic |
| ACTCACAGCGGCAACAATGG+TGG | 0.673138 | 8.3:+10313903 | MS.gene57993:CDS |
| CCTCGCTTACCTCCTCATAG+CGG | 0.734427 | 8.3:+10314020 | MS.gene57993:CDS |
| CAGCAGCACAGTACTCACAG+CGG | 0.771096 | 8.3:+10313891 | MS.gene57993:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAAAGAAAAAAAAAAA+AGG | + | chr8.3:10313741-10313760 | MS.gene57993:intron | 10.0% |
| !! | AAAACACTTAATACTAAATA+GGG | - | chr8.3:10314261-10314280 | None:intergenic | 15.0% |
| !! | TAAAACACTTAATACTAAAT+AGG | - | chr8.3:10314262-10314281 | None:intergenic | 15.0% |
| !! | TATAATCATAGATACAAAAA+AGG | - | chr8.3:10314578-10314597 | None:intergenic | 15.0% |
| !! | AACAAATTCGTATAACTAAA+AGG | - | chr8.3:10313090-10313109 | None:intergenic | 20.0% |
| !! | ATAGATACAAAAAAGGTAAA+AGG | - | chr8.3:10314571-10314590 | None:intergenic | 20.0% |
| !!! | AAAGGAATTTTAACATCAAA+CGG | - | chr8.3:10313278-10313297 | None:intergenic | 20.0% |
| !!! | GTATTAAGTGTTTTAAATAG+CGG | + | chr8.3:10314267-10314286 | MS.gene57993:intron | 20.0% |
| !!! | TTTCTTCTTTGAAATTTGAA+AGG | - | chr8.3:10313296-10313315 | None:intergenic | 20.0% |
| ! | AAAACTCGTTCTTTAATCTA+GGG | - | chr8.3:10314125-10314144 | None:intergenic | 25.0% |
| ! | AAAGTCATTGATGTTAACAT+CGG | + | chr8.3:10313218-10313237 | MS.gene57993:intron | 25.0% |
| ! | AATTCGTATAACTAAAAGGT+AGG | - | chr8.3:10313086-10313105 | None:intergenic | 25.0% |
| ! | ACAACAATAGCAACAAAAAA+GGG | - | chr8.3:10313570-10313589 | None:intergenic | 25.0% |
| ! | ATTTGAATGAAAAACCCTAA+CGG | + | chr8.3:10313816-10313835 | MS.gene57993:intron | 25.0% |
| ! | CACATTATCTACTAAATTGT+TGG | - | chr8.3:10314421-10314440 | None:intergenic | 25.0% |
| ! | TAAAACTCGTTCTTTAATCT+AGG | - | chr8.3:10314126-10314145 | None:intergenic | 25.0% |
| ! | TACAACAATAGCAACAAAAA+AGG | - | chr8.3:10313571-10313590 | None:intergenic | 25.0% |
| ! | TAGTAGATAATGTGTGAAAA+GGG | + | chr8.3:10314427-10314446 | MS.gene57993:intron | 25.0% |
| ! | TTAGTAGATAATGTGTGAAA+AGG | + | chr8.3:10314426-10314445 | MS.gene57993:intron | 25.0% |
| ! | TTCATATCAAGAATCTAGTA+TGG | + | chr8.3:10313673-10313692 | MS.gene57993:intron | 25.0% |
| ! | TTCCTGATAAATAACATTTG+AGG | - | chr8.3:10313510-10313529 | None:intergenic | 25.0% |
| ! | TTGAAAATACTAATCGTTGA+TGG | + | chr8.3:10313461-10313480 | MS.gene57993:intron | 25.0% |
| !! | AACCTCAAATGTTATTTATC+AGG | + | chr8.3:10313505-10313524 | MS.gene57993:intron | 25.0% |
| !! | TGAAATCTGATAGTTTTTGT+TGG | + | chr8.3:10314669-10314688 | MS.gene57993:intron | 25.0% |
| !! | TTGCTATTGTTGTATTAGAA+GGG | + | chr8.3:10313577-10313596 | MS.gene57993:intron | 25.0% |
| !!! | TTGCTAATTTGCTTTTCAAT+AGG | + | chr8.3:10314833-10314852 | MS.gene57993:intron | 25.0% |
| AGAGATCAAAATGTCTTTGA+AGG | - | chr8.3:10313032-10313051 | None:intergenic | 30.0% | |
| AGTCACTTGTATCAAATCAT+TGG | - | chr8.3:10314155-10314174 | None:intergenic | 30.0% | |
| ATGATTACCTCTAAGTTGTT+TGG | + | chr8.3:10314393-10314412 | MS.gene57993:intron | 30.0% | |
| ATGATTTGATACAAGTGACT+AGG | + | chr8.3:10314155-10314174 | MS.gene57993:intron | 30.0% | |
| CAAAATGACAATTGGACAAT+TGG | - | chr8.3:10314624-10314643 | None:intergenic | 30.0% | |
| CAATCATTTGATATCACCAA+TGG | + | chr8.3:10314957-10314976 | MS.gene57993:CDS | 30.0% | |
| CTAGAAAAATCTCCTAAACT+TGG | - | chr8.3:10314474-10314493 | None:intergenic | 30.0% | |
| GAACTGAAAAATTGTGAGAT+AGG | - | chr8.3:10314053-10314072 | None:intergenic | 30.0% | |
| TGATTTGATACAAGTGACTA+GGG | + | chr8.3:10314156-10314175 | MS.gene57993:intron | 30.0% | |
| TTAGAGGTAATCATTAGAGA+GGG | - | chr8.3:10314387-10314406 | None:intergenic | 30.0% | |
| TTTGAATGAAAAACCCTAAC+GGG | + | chr8.3:10313817-10313836 | MS.gene57993:intron | 30.0% | |
| ! | GTTGCTATTGTTGTATTAGA+AGG | + | chr8.3:10313576-10313595 | MS.gene57993:intron | 30.0% |
| !! | AGAAATTTGTTGCCAAGTTT+AGG | + | chr8.3:10314459-10314478 | MS.gene57993:intron | 30.0% |
| !!! | CTAATTTGCTTTTCAATAGG+TGG | + | chr8.3:10314836-10314855 | MS.gene57993:intron | 30.0% |
| !!! | TAGGAGATTTTTCTAGAACA+GGG | + | chr8.3:10314478-10314497 | MS.gene57993:intron | 30.0% |
| !!! | TTAGGAGATTTTTCTAGAAC+AGG | + | chr8.3:10314477-10314496 | MS.gene57993:intron | 30.0% |
| !!! | TTGTCCAATTGTCATTTTGT+CGG | + | chr8.3:10314625-10314644 | MS.gene57993:intron | 30.0% |
| !!! | TTTTTTGTTGTAGATGTCCT+TGG | + | chr8.3:10313140-10313159 | MS.gene57993:intron | 30.0% |
| AAATATTAAACGTACCCACG+TGG | + | chr8.3:10313765-10313784 | MS.gene57993:intron | 35.0% | |
| AACGACTTCTTCAAATTCTG+CGG | - | chr8.3:10314311-10314330 | None:intergenic | 35.0% | |
| ATGACAATCAACAAGAGTCA+AGG | + | chr8.3:10313341-10313360 | MS.gene57993:intron | 35.0% | |
| CATTTGATATCACCAATGGA+TGG | + | chr8.3:10314961-10314980 | MS.gene57993:CDS | 35.0% | |
| CTTAGAGGTAATCATTAGAG+AGG | - | chr8.3:10314388-10314407 | None:intergenic | 35.0% | |
| GCTTTAATGTTAAAGCGTAG+TGG | + | chr8.3:10314204-10314223 | MS.gene57993:intron | 35.0% | |
| GGGCAATCACTTAAAAATGT+TGG | + | chr8.3:10313362-10313381 | MS.gene57993:intron | 35.0% | |
| GTAAGAAAAACCGAGAAGAA+TGG | + | chr8.3:10313185-10313204 | MS.gene57993:intron | 35.0% | |
| TCAGATTTCACAGTAATGAG+CGG | - | chr8.3:10314659-10314678 | None:intergenic | 35.0% | |
| TGACAATCAACAAGAGTCAA+GGG | + | chr8.3:10313342-10313361 | MS.gene57993:intron | 35.0% | |
| ! | TTGACTCTTGTTGATTGTCA+TGG | - | chr8.3:10313342-10313361 | None:intergenic | 35.0% |
| !! | CAAAGACATTTTGATCTCTC+AGG | + | chr8.3:10313033-10313052 | MS.gene57993:CDS | 35.0% |
| !! | GCTTTTTCTTCCATTCTTCT+CGG | - | chr8.3:10313198-10313217 | None:intergenic | 35.0% |
| !!! | GGTCTTATTTTGTTTGTGTC+TGG | + | chr8.3:10314499-10314518 | MS.gene57993:intron | 35.0% |
| !!! | GTTTGTTTTTTTCTCCAGAC+TGG | + | chr8.3:10314916-10314935 | MS.gene57993:intron | 35.0% |
| !!! | TGTCCAATTGTCATTTTGTC+GGG | + | chr8.3:10314626-10314645 | MS.gene57993:intron | 35.0% |
| AAACGGTGAAAGAGATAACC+TGG | - | chr8.3:10313261-10313280 | None:intergenic | 40.0% | |
| AACGGTGAAAGAGATAACCT+GGG | - | chr8.3:10313260-10313279 | None:intergenic | 40.0% | |
| AAGCCCGACAAAATGACAAT+TGG | - | chr8.3:10314632-10314651 | None:intergenic | 40.0% | |
| AGATTTCACAGTAATGAGCG+GGG | - | chr8.3:10314657-10314676 | None:intergenic | 40.0% | |
| ATACAAGTGACTAGGGTTTG+TGG | + | chr8.3:10314163-10314182 | MS.gene57993:intron | 40.0% | |
| ATGAATTCAAACACGTTCCG+AGG | - | chr8.3:10313007-10313026 | None:intergenic | 40.0% | |
| CAAATTGTTTGCTTCCACGT+GGG | - | chr8.3:10313782-10313801 | None:intergenic | 40.0% | |
| CAGATTTCACAGTAATGAGC+GGG | - | chr8.3:10314658-10314677 | None:intergenic | 40.0% | |
| GATCTGCAATTGACAACACT+AGG | + | chr8.3:10314340-10314359 | MS.gene57993:intron | 40.0% | |
| GGGAAATGCGAGATTTACTA+AGG | + | chr8.3:10312964-10312983 | MS.gene57993:CDS | 40.0% | |
| TCCAGAGTCACTTCAAAACA+AGG | + | chr8.3:10313437-10313456 | MS.gene57993:intron | 40.0% | |
| TGAATGCAAACAATGTGCCT+CGG | + | chr8.3:10312987-10313006 | MS.gene57993:CDS | 40.0% | |
| ! | ATAGGAAGTATACCGCTATG+AGG | - | chr8.3:10314035-10314054 | None:intergenic | 40.0% |
| ! | GCGAAAAAGTTTTCATACCC+AGG | + | chr8.3:10313240-10313259 | MS.gene57993:intron | 40.0% |
| !! | GATCGAGTTGTTGTTTCCAA+AGG | - | chr8.3:10313941-10313960 | None:intergenic | 40.0% |
| !!! | ACCTTGTTTTGAAGTGACTC+TGG | - | chr8.3:10313441-10313460 | None:intergenic | 40.0% |
| !!! | TTGCATACCTCCTTTTTTGC+TGG | + | chr8.3:10314730-10314749 | MS.gene57993:intron | 40.0% |
| ACAGCTTCCAGCAAAAAAGG+AGG | - | chr8.3:10314740-10314759 | None:intergenic | 45.0% | |
| CAGTAAGAAGAGATCCAGTC+TGG | - | chr8.3:10314933-10314952 | None:intergenic | 45.0% | |
| CCAAATTGTTTGCTTCCACG+TGG | - | chr8.3:10313783-10313802 | None:intergenic | 45.0% | |
| CCACGTGGAAGCAAACAATT+TGG | + | chr8.3:10313780-10313799 | MS.gene57993:intron | 45.0% | |
| CGTACAGCTTCCAGCAAAAA+AGG | - | chr8.3:10314743-10314762 | None:intergenic | 45.0% | |
| CTTCGCTAGAGAAATCGAGA+GGG | - | chr8.3:10313868-10313887 | None:intergenic | 45.0% | |
| CTTTCTCGACTACATGACCA+AGG | - | chr8.3:10313160-10313179 | None:intergenic | 45.0% | |
| ! | GTGGTTTGAGACATTCGTAG+TGG | + | chr8.3:10314182-10314201 | MS.gene57993:intron | 45.0% |
| !! | GTTGGTGCCAAACAACTTAG+AGG | - | chr8.3:10314403-10314422 | None:intergenic | 45.0% |
| AGTACTCACAGCGGCAACAA+TGG | + | chr8.3:10313900-10313919 | MS.gene57993:CDS | 50.0% | |
| CCATGCGAGAACACAAGACT+CGG | - | chr8.3:10313398-10313417 | None:intergenic | 50.0% | |
| GATCTCTCAGGAAGATGACG+TGG | + | chr8.3:10313045-10313064 | MS.gene57993:CDS | 50.0% | |
| GCTTCGCTAGAGAAATCGAG+AGG | - | chr8.3:10313869-10313888 | None:intergenic | 50.0% | |
| GTGGTTTCATCTCTCGCCTT+TGG | + | chr8.3:10313922-10313941 | MS.gene57993:CDS | 50.0% | |
| ! | ACTTTGGGTTTGGTCCCGTT+AGG | - | chr8.3:10313834-10313853 | None:intergenic | 50.0% |
| ! | AGAAATCGAGAGGGTGTGTG+CGG | - | chr8.3:10313859-10313878 | None:intergenic | 50.0% |
| ! | CTTTGGGTTTGGTCCCGTTA+GGG | - | chr8.3:10313833-10313852 | None:intergenic | 50.0% |
| ! | GGAAGTATACCGCTATGAGG+AGG | - | chr8.3:10314032-10314051 | None:intergenic | 50.0% |
| ACTCACAGCGGCAACAATGG+TGG | + | chr8.3:10313903-10313922 | MS.gene57993:CDS | 55.0% | |
| AGGGTGTGTGCGGGAACTTT+GGG | - | chr8.3:10313849-10313868 | None:intergenic | 55.0% | |
| CAGCAGCACAGTACTCACAG+CGG | + | chr8.3:10313891-10313910 | MS.gene57993:CDS | 55.0% | |
| CCTCGCTTACCTCCTCATAG+CGG | + | chr8.3:10314020-10314039 | MS.gene57993:CDS | 55.0% | |
| CGCTATGAGGAGGTAAGCGA+GGG | - | chr8.3:10314022-10314041 | None:intergenic | 55.0% | |
| GAGGAGGTAAGCGAGGGATT+TGG | - | chr8.3:10314016-10314035 | None:intergenic | 55.0% | |
| ! | CCGAGTCTTGTGTTCTCGCA+TGG | + | chr8.3:10313395-10313414 | MS.gene57993:intron | 55.0% |
| ! | GAAATCGAGAGGGTGTGTGC+GGG | - | chr8.3:10313858-10313877 | None:intergenic | 55.0% |
| ! | GTGTGCGGGAACTTTGGGTT+TGG | - | chr8.3:10313844-10313863 | None:intergenic | 55.0% |
| CCGCTATGAGGAGGTAAGCG+AGG | - | chr8.3:10314023-10314042 | None:intergenic | 60.0% | |
| ! | GAGGGTGTGTGCGGGAACTT+TGG | - | chr8.3:10313850-10313869 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 10312962 | 10314986 | 10312962 | ID=MS.gene57993 |
| chr8.3 | mRNA | 10312962 | 10314986 | 10312962 | ID=MS.gene57993.t1;Parent=MS.gene57993 |
| chr8.3 | exon | 10312962 | 10313066 | 10312962 | ID=MS.gene57993.t1.exon1;Parent=MS.gene57993.t1 |
| chr8.3 | CDS | 10312962 | 10313066 | 10312962 | ID=cds.MS.gene57993.t1;Parent=MS.gene57993.t1 |
| chr8.3 | exon | 10313883 | 10314041 | 10313883 | ID=MS.gene57993.t1.exon2;Parent=MS.gene57993.t1 |
| chr8.3 | CDS | 10313883 | 10314041 | 10313883 | ID=cds.MS.gene57993.t1;Parent=MS.gene57993.t1 |
| chr8.3 | exon | 10314934 | 10314986 | 10314934 | ID=MS.gene57993.t1.exon3;Parent=MS.gene57993.t1 |
| chr8.3 | CDS | 10314934 | 10314986 | 10314934 | ID=cds.MS.gene57993.t1;Parent=MS.gene57993.t1 |
| Gene Sequence |
| Protein sequence |
