Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58328.t1 | XP_003627627.1 | 97.3 | 148 | 4 | 0 | 1 | 148 | 1 | 148 | 5.50E-76 | 293.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58328.t1 | Q9C8S6 | 57.4 | 148 | 62 | 1 | 1 | 148 | 1 | 147 | 2.6e-42 | 172.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58328.t1 | G7LCN6 | 97.3 | 148 | 4 | 0 | 1 | 148 | 1 | 148 | 3.9e-76 | 293.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059802 | MS.gene58328 | 0.801309 | 9.57E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58328.t1 | MTR_8g032320 | 97.297 | 148 | 4 | 0 | 1 | 148 | 1 | 148 | 1.16e-104 | 295 |
| MS.gene58328.t1 | MTR_8g032300 | 85.135 | 148 | 22 | 0 | 1 | 148 | 1 | 148 | 2.84e-94 | 269 |
| MS.gene58328.t1 | MTR_1g008950 | 63.200 | 125 | 46 | 0 | 1 | 125 | 1 | 125 | 1.36e-57 | 176 |
| MS.gene58328.t1 | MTR_5g079880 | 41.290 | 155 | 76 | 4 | 1 | 144 | 1 | 151 | 4.43e-38 | 127 |
| MS.gene58328.t1 | MTR_5g079890 | 40.506 | 158 | 78 | 5 | 1 | 145 | 1 | 155 | 4.49e-33 | 114 |
| MS.gene58328.t1 | MTR_5g079890 | 40.580 | 138 | 69 | 4 | 1 | 125 | 1 | 138 | 1.92e-29 | 104 |
| MS.gene58328.t1 | MTR_7g075960 | 56.604 | 53 | 23 | 0 | 25 | 77 | 1 | 53 | 1.22e-19 | 77.4 |
| MS.gene58328.t1 | MTR_8g030870 | 32.432 | 148 | 86 | 4 | 1 | 135 | 1 | 147 | 4.09e-19 | 79.7 |
| MS.gene58328.t1 | MTR_5g004800 | 26.190 | 126 | 91 | 2 | 6 | 130 | 15 | 139 | 7.97e-13 | 63.9 |
| MS.gene58328.t1 | MTR_3g106660 | 33.071 | 127 | 79 | 4 | 3 | 127 | 439 | 561 | 2.32e-12 | 63.5 |
| MS.gene58328.t1 | MTR_5g004870 | 28.571 | 119 | 83 | 2 | 6 | 123 | 11 | 128 | 2.80e-12 | 62.8 |
| MS.gene58328.t1 | MTR_2g027100 | 37.500 | 96 | 58 | 2 | 4 | 99 | 6 | 99 | 3.75e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58328.t1 | AT1G63220 | 57.432 | 148 | 62 | 1 | 1 | 148 | 1 | 147 | 6.13e-57 | 174 |
| MS.gene58328.t1 | AT1G63220 | 53.378 | 148 | 54 | 2 | 1 | 148 | 1 | 133 | 9.03e-48 | 151 |
| MS.gene58328.t1 | AT3G55470 | 36.709 | 158 | 86 | 4 | 1 | 147 | 1 | 155 | 1.05e-26 | 98.2 |
| MS.gene58328.t1 | AT3G55470 | 37.107 | 159 | 85 | 5 | 1 | 147 | 1 | 156 | 1.10e-25 | 95.9 |
| MS.gene58328.t1 | AT4G00467 | 32.331 | 133 | 79 | 3 | 1 | 122 | 1 | 133 | 4.19e-17 | 74.3 |
| MS.gene58328.t1 | AT4G00467 | 32.331 | 133 | 79 | 3 | 1 | 122 | 1 | 133 | 6.72e-17 | 74.3 |
| MS.gene58328.t1 | AT5G04220 | 30.380 | 158 | 95 | 5 | 2 | 147 | 37 | 191 | 3.70e-13 | 65.5 |
| MS.gene58328.t1 | AT5G47710 | 37.755 | 98 | 59 | 2 | 2 | 99 | 4 | 99 | 4.03e-13 | 63.5 |
| MS.gene58328.t1 | AT5G47710 | 37.755 | 98 | 59 | 2 | 2 | 99 | 4 | 99 | 4.03e-13 | 63.5 |
| MS.gene58328.t1 | AT5G47710 | 37.755 | 98 | 59 | 2 | 2 | 99 | 4 | 99 | 4.03e-13 | 63.5 |
| MS.gene58328.t1 | AT5G47710 | 37.755 | 98 | 59 | 2 | 2 | 99 | 4 | 99 | 4.03e-13 | 63.5 |
| MS.gene58328.t1 | AT5G04220 | 30.380 | 158 | 95 | 5 | 2 | 147 | 197 | 351 | 8.12e-13 | 65.1 |
| MS.gene58328.t1 | AT4G34150 | 27.966 | 118 | 83 | 2 | 6 | 122 | 12 | 128 | 9.52e-13 | 63.9 |
| MS.gene58328.t1 | AT5G04220 | 29.730 | 148 | 91 | 4 | 2 | 137 | 259 | 405 | 1.15e-12 | 64.7 |
Find 25 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATATTGTGGAGAGATTA+AGG | 0.258182 | 8.2:+65806515 | MS.gene58328:CDS |
| TATAATAATGATGATAATAT+TGG | 0.281672 | 8.2:+65805964 | MS.gene58328:CDS |
| TGAGCTCAATCTAAAGATAA+TGG | 0.346296 | 8.2:+65805930 | MS.gene58328:CDS |
| ACAAGATTGTAAGAATTCTC+TGG | 0.392769 | 8.2:-65806483 | None:intergenic |
| GCTCCTGTGCTTTGTAACTA+AGG | 0.446848 | 8.2:-65805729 | None:intergenic |
| GAAAAGTCTCATTCCATTGA+GGG | 0.461869 | 8.2:-65805882 | None:intergenic |
| CAATCTAAAGATAATGGAAA+AGG | 0.492449 | 8.2:+65805936 | MS.gene58328:CDS |
| TCTTCAAACACTGCTTCTAA+AGG | 0.503608 | 8.2:-65806450 | None:intergenic |
| GTGCTGGATCTAACCCTCAA+TGG | 0.522113 | 8.2:+65805869 | MS.gene58328:CDS |
| AGAAAAGTCTCATTCCATTG+AGG | 0.522164 | 8.2:-65805883 | None:intergenic |
| AAGAGGAGGAGACTGGTGGA+TGG | 0.524498 | 8.2:+65806683 | MS.gene58328:CDS |
| TACAATGAAGAGGAGGAGAC+TGG | 0.545609 | 8.2:+65806676 | MS.gene58328:CDS |
| TTGTAACTAAGGATCACATA+AGG | 0.555757 | 8.2:-65805718 | None:intergenic |
| TTAGAAGCAGTGTTTGAAGA+GGG | 0.587548 | 8.2:+65806453 | MS.gene58328:CDS |
| CACAAGAGTACTGTTCAAGA+AGG | 0.590870 | 8.2:+65805751 | MS.gene58328:CDS |
| GTGAAGGATGAAGAATATTG+TGG | 0.592520 | 8.2:+65806504 | MS.gene58328:CDS |
| GATCCTTAGTTACAAAGCAC+AGG | 0.599886 | 8.2:+65805726 | MS.gene58328:CDS |
| TGATCAGAGTTACAATGAAG+AGG | 0.605643 | 8.2:+65806666 | MS.gene58328:CDS |
| TTTAGAAGCAGTGTTTGAAG+AGG | 0.610475 | 8.2:+65806452 | MS.gene58328:CDS |
| GAATTCTTACAATCTTGTGA+AGG | 0.613624 | 8.2:+65806488 | MS.gene58328:CDS |
| TCAGAGTTACAATGAAGAGG+AGG | 0.621822 | 8.2:+65806669 | MS.gene58328:CDS |
| AATGAAGAGGAGGAGACTGG+TGG | 0.640663 | 8.2:+65806679 | MS.gene58328:CDS |
| GATTGTAAGAATTCTCTGGA+AGG | 0.642476 | 8.2:-65806479 | None:intergenic |
| ACTCAAAGAAAGATGCCTCG+TGG | 0.682508 | 8.2:+65805230 | None:intergenic |
| ATAACTTCAAGAGTTCCACG+AGG | 0.830018 | 8.2:-65805245 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAATAATGATGATAATAT+TGG | + | chr8.2:65805964-65805983 | MS.gene58328:CDS | 10.0% |
| !!! | TAATAATCAAATTATTTTTC+AGG | - | chr8.2:65805469-65805488 | None:intergenic | 10.0% |
| !! | TTAAATGTACTATCAATTAT+TGG | + | chr8.2:65806629-65806648 | MS.gene58328:intron | 15.0% |
| !!! | AATTGATAGTACATTTAATA+GGG | - | chr8.2:65806627-65806646 | None:intergenic | 15.0% |
| !!! | ATCTTTTTATGAATAATATC+AGG | + | chr8.2:65805847-65805866 | MS.gene58328:intron | 15.0% |
| !!! | TAATTGATAGTACATTTAAT+AGG | - | chr8.2:65806628-65806647 | None:intergenic | 15.0% |
| !! | CACTTTAAATCATATCTAAA+TGG | - | chr8.2:65806338-65806357 | None:intergenic | 20.0% |
| !! | GAATGAAATATAAACAAAAG+TGG | - | chr8.2:65806191-65806210 | None:intergenic | 20.0% |
| !! | GTTTAAAAATTTACACCTAA+AGG | + | chr8.2:65806270-65806289 | MS.gene58328:intron | 20.0% |
| !! | TGAAATATAAGCAAAAAAGT+AGG | + | chr8.2:65806118-65806137 | MS.gene58328:intron | 20.0% |
| !! | TTCTCTAATTCAATTAGAAA+AGG | - | chr8.2:65805571-65805590 | None:intergenic | 20.0% |
| !! | TTGAATTAGAGAATCAATTT+AGG | + | chr8.2:65805578-65805597 | MS.gene58328:intron | 20.0% |
| !!! | AAACATAAGTAGTATTGATT+TGG | + | chr8.2:65805786-65805805 | MS.gene58328:intron | 20.0% |
| !!! | CTTTTGTTTATATTTCATTC+CGG | + | chr8.2:65806191-65806210 | MS.gene58328:intron | 20.0% |
| !!! | TATTTCTATGTACTATACAT+AGG | + | chr8.2:65805314-65805333 | MS.gene58328:intron | 20.0% |
| !!! | TTTTTGCTTATATTTCATTC+TGG | - | chr8.2:65806115-65806134 | None:intergenic | 20.0% |
| ! | AAAAATGAAAGTCAAACTCA+TGG | - | chr8.2:65805426-65805445 | None:intergenic | 25.0% |
| ! | AAAAATTTACACCTAAAGGA+GGG | + | chr8.2:65806274-65806293 | MS.gene58328:intron | 25.0% |
| ! | AGAAATTATTCATGCTAAGT+TGG | + | chr8.2:65805495-65805514 | MS.gene58328:intron | 25.0% |
| ! | TAAAAATTTACACCTAAAGG+AGG | + | chr8.2:65806273-65806292 | MS.gene58328:intron | 25.0% |
| ! | TTATGTAGAATTATTACCTC+AGG | - | chr8.2:65806558-65806577 | None:intergenic | 25.0% |
| !! | TGTCTTTGATTCATCTTTTT+AGG | + | chr8.2:65806026-65806045 | MS.gene58328:intron | 25.0% |
| !!! | CAATCTAAAGATAATGGAAA+AGG | + | chr8.2:65805936-65805955 | MS.gene58328:CDS | 25.0% |
| !!! | TGAGTTTGACTTTCATTTTT+TGG | + | chr8.2:65805426-65805445 | MS.gene58328:intron | 25.0% |
| !!! | TTGTTATTTTTGTTCTCTAG+TGG | + | chr8.2:65805813-65805832 | MS.gene58328:intron | 25.0% |
| ACAAGATTGTAAGAATTCTC+TGG | - | chr8.2:65806486-65806505 | None:intergenic | 30.0% | |
| ACTTTAACACTTACTTTCTC+CGG | - | chr8.2:65806213-65806232 | None:intergenic | 30.0% | |
| AGAATATTGTGGAGAGATTA+AGG | + | chr8.2:65806515-65806534 | MS.gene58328:CDS | 30.0% | |
| GAAATTTCTGCATGAAACAA+TGG | + | chr8.2:65805398-65805417 | MS.gene58328:intron | 30.0% | |
| GAATTCTTACAATCTTGTGA+AGG | + | chr8.2:65806488-65806507 | MS.gene58328:CDS | 30.0% | |
| GAGAAAGTAAGTGTTAAAGT+TGG | + | chr8.2:65806213-65806232 | MS.gene58328:intron | 30.0% | |
| GTTTGACTATGTAACTTCAA+AGG | - | chr8.2:65806364-65806383 | None:intergenic | 30.0% | |
| TGAGCTCAATCTAAAGATAA+TGG | + | chr8.2:65805930-65805949 | MS.gene58328:CDS | 30.0% | |
| TTATGAATAATATCAGGTGC+TGG | + | chr8.2:65805853-65805872 | MS.gene58328:intron | 30.0% | |
| TTGCTTATATTTCATTCTGG+AGG | - | chr8.2:65806112-65806131 | None:intergenic | 30.0% | |
| TTGTAACTAAGGATCACATA+AGG | - | chr8.2:65805721-65805740 | None:intergenic | 30.0% | |
| !!! | GTTATTTTGATCAGTGCTAA+AGG | + | chr8.2:65805263-65805282 | MS.gene58328:CDS | 30.0% |
| AAAATTGAACGAGATGTCAG+TGG | - | chr8.2:65806392-65806411 | None:intergenic | 35.0% | |
| AGAAAAGTCTCATTCCATTG+AGG | - | chr8.2:65805886-65805905 | None:intergenic | 35.0% | |
| GAAAAGTCTCATTCCATTGA+GGG | - | chr8.2:65805885-65805904 | None:intergenic | 35.0% | |
| GATTGTAAGAATTCTCTGGA+AGG | - | chr8.2:65806482-65806501 | None:intergenic | 35.0% | |
| GTGAAGGATGAAGAATATTG+TGG | + | chr8.2:65806504-65806523 | MS.gene58328:CDS | 35.0% | |
| GTTGGATTCTCATTGAAAGT+TGG | + | chr8.2:65806231-65806250 | MS.gene58328:intron | 35.0% | |
| TCTTCAAACACTGCTTCTAA+AGG | - | chr8.2:65806453-65806472 | None:intergenic | 35.0% | |
| TGATCAGAGTTACAATGAAG+AGG | + | chr8.2:65806666-65806685 | MS.gene58328:CDS | 35.0% | |
| TTAGAAGCAGTGTTTGAAGA+GGG | + | chr8.2:65806453-65806472 | MS.gene58328:CDS | 35.0% | |
| TTTAGAAGCAGTGTTTGAAG+AGG | + | chr8.2:65806452-65806471 | MS.gene58328:CDS | 35.0% | |
| ATAACTTCAAGAGTTCCACG+AGG | - | chr8.2:65805248-65805267 | None:intergenic | 40.0% | |
| GATCCTTAGTTACAAAGCAC+AGG | + | chr8.2:65805726-65805745 | MS.gene58328:CDS | 40.0% | |
| TCAGAGTTACAATGAAGAGG+AGG | + | chr8.2:65806669-65806688 | MS.gene58328:CDS | 40.0% | |
| !! | CACAAGAGTACTGTTCAAGA+AGG | + | chr8.2:65805751-65805770 | MS.gene58328:CDS | 40.0% |
| AGTTGCAAAGTCGACGTATG+TGG | - | chr8.2:65806167-65806186 | None:intergenic | 45.0% | |
| GCTCCTGTGCTTTGTAACTA+AGG | - | chr8.2:65805732-65805751 | None:intergenic | 45.0% | |
| TACAATGAAGAGGAGGAGAC+TGG | + | chr8.2:65806676-65806695 | MS.gene58328:CDS | 45.0% | |
| !! | TGCTCTCACTTTTACACCTG+AGG | + | chr8.2:65806539-65806558 | MS.gene58328:CDS | 45.0% |
| AATGAAGAGGAGGAGACTGG+TGG | + | chr8.2:65806679-65806698 | MS.gene58328:CDS | 50.0% | |
| CTTCCTTTGTGCCCTCCTTT+AGG | - | chr8.2:65806288-65806307 | None:intergenic | 50.0% | |
| GTGCTGGATCTAACCCTCAA+TGG | + | chr8.2:65805869-65805888 | MS.gene58328:CDS | 50.0% | |
| ! | ACACCTAAAGGAGGGCACAA+AGG | + | chr8.2:65806282-65806301 | MS.gene58328:intron | 50.0% |
| AAGAGGAGGAGACTGGTGGA+TGG | + | chr8.2:65806683-65806702 | MS.gene58328:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 65805242 | 65806729 | 65805242 | ID=MS.gene58328 |
| chr8.2 | mRNA | 65805242 | 65806729 | 65805242 | ID=MS.gene58328.t1;Parent=MS.gene58328 |
| chr8.2 | exon | 65805242 | 65805308 | 65805242 | ID=MS.gene58328.t1.exon1;Parent=MS.gene58328.t1 |
| chr8.2 | CDS | 65805242 | 65805308 | 65805242 | ID=cds.MS.gene58328.t1;Parent=MS.gene58328.t1 |
| chr8.2 | exon | 65805707 | 65805772 | 65805707 | ID=MS.gene58328.t1.exon2;Parent=MS.gene58328.t1 |
| chr8.2 | CDS | 65805707 | 65805772 | 65805707 | ID=cds.MS.gene58328.t1;Parent=MS.gene58328.t1 |
| chr8.2 | exon | 65805869 | 65805995 | 65805869 | ID=MS.gene58328.t1.exon3;Parent=MS.gene58328.t1 |
| chr8.2 | CDS | 65805869 | 65805995 | 65805869 | ID=cds.MS.gene58328.t1;Parent=MS.gene58328.t1 |
| chr8.2 | exon | 65806446 | 65806560 | 65806446 | ID=MS.gene58328.t1.exon4;Parent=MS.gene58328.t1 |
| chr8.2 | CDS | 65806446 | 65806560 | 65806446 | ID=cds.MS.gene58328.t1;Parent=MS.gene58328.t1 |
| chr8.2 | exon | 65806658 | 65806729 | 65806658 | ID=MS.gene58328.t1.exon5;Parent=MS.gene58328.t1 |
| chr8.2 | CDS | 65806658 | 65806729 | 65806658 | ID=cds.MS.gene58328.t1;Parent=MS.gene58328.t1 |
| Gene Sequence |
| Protein sequence |
