Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58510.t1 | XP_003609672.1 | 97.1 | 454 | 13 | 0 | 1 | 454 | 1 | 454 | 4.20E-268 | 933.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58510.t1 | O22775 | 80.1 | 462 | 80 | 3 | 1 | 453 | 1 | 459 | 1.8e-227 | 789.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58510.t1 | G7JLH4 | 97.1 | 454 | 13 | 0 | 1 | 454 | 1 | 454 | 3.0e-268 | 933.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055531 | MS.gene58510 | 0.830038 | 3.68E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58510.t1 | MTR_4g119880 | 98.899 | 454 | 5 | 0 | 1 | 454 | 1 | 454 | 0.0 | 943 |
| MS.gene58510.t1 | MTR_7g108675 | 78.373 | 467 | 87 | 3 | 1 | 454 | 1 | 466 | 0.0 | 754 |
| MS.gene58510.t1 | MTR_8g058553 | 78.072 | 415 | 77 | 3 | 1 | 402 | 1 | 414 | 0.0 | 669 |
| MS.gene58510.t1 | MTR_3g037340 | 58.469 | 431 | 154 | 4 | 10 | 440 | 36 | 441 | 0.0 | 546 |
| MS.gene58510.t1 | MTR_4g108940 | 57.240 | 442 | 165 | 4 | 8 | 449 | 28 | 445 | 0.0 | 545 |
| MS.gene58510.t1 | MTR_5g015260 | 42.526 | 388 | 181 | 7 | 79 | 431 | 66 | 446 | 8.83e-111 | 334 |
| MS.gene58510.t1 | MTR_4g097240 | 39.486 | 428 | 206 | 8 | 27 | 421 | 19 | 426 | 1.93e-108 | 328 |
| MS.gene58510.t1 | MTR_4g097230 | 43.466 | 352 | 160 | 6 | 107 | 424 | 69 | 415 | 1.37e-101 | 310 |
| MS.gene58510.t1 | MTR_4g108950 | 49.606 | 127 | 20 | 1 | 285 | 411 | 1 | 83 | 2.66e-33 | 121 |
| MS.gene58510.t1 | MTR_4g132910 | 82.051 | 39 | 7 | 0 | 357 | 395 | 582 | 620 | 2.16e-13 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58510.t1 | AT3G62720 | 77.874 | 461 | 93 | 4 | 1 | 454 | 1 | 459 | 0.0 | 763 |
| MS.gene58510.t1 | AT3G62720 | 77.874 | 461 | 93 | 4 | 1 | 454 | 1 | 459 | 0.0 | 763 |
| MS.gene58510.t1 | AT4G02500 | 79.914 | 463 | 81 | 3 | 1 | 454 | 1 | 460 | 0.0 | 761 |
| MS.gene58510.t1 | AT1G74380 | 58.879 | 428 | 153 | 5 | 13 | 440 | 42 | 446 | 0.0 | 553 |
| MS.gene58510.t1 | AT5G07720 | 58.469 | 431 | 155 | 5 | 10 | 440 | 39 | 445 | 0.0 | 551 |
| MS.gene58510.t1 | AT1G18690 | 52.292 | 480 | 185 | 8 | 4 | 440 | 26 | 504 | 4.90e-180 | 513 |
| MS.gene58510.t1 | AT1G18690 | 52.292 | 480 | 185 | 8 | 4 | 440 | 26 | 504 | 4.90e-180 | 513 |
| MS.gene58510.t1 | AT1G18690 | 52.292 | 480 | 185 | 8 | 4 | 440 | 26 | 504 | 4.90e-180 | 513 |
| MS.gene58510.t1 | AT1G18690 | 52.292 | 480 | 185 | 8 | 4 | 440 | 26 | 504 | 4.90e-180 | 513 |
| MS.gene58510.t1 | AT2G22900 | 42.120 | 349 | 159 | 5 | 107 | 417 | 89 | 432 | 1.92e-96 | 297 |
| MS.gene58510.t1 | AT4G37690 | 40.974 | 349 | 163 | 6 | 107 | 417 | 72 | 415 | 1.35e-94 | 292 |
| MS.gene58510.t1 | AT4G38310 | 44.444 | 108 | 56 | 2 | 107 | 213 | 13 | 117 | 6.40e-28 | 107 |
Find 0 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTAGGATTAATAAAATT+AGG | + | 32591:690-709 | MS.gene58510:intergenic | 10.0% |
| !! | TAATAAAATTAGGATAATCA+GGG | + | 32591:680-699 | MS.gene58510:intergenic | 15.0% |
| !! | TTAATAAAATTAGGATAATC+AGG | + | 32591:681-700 | MS.gene58510:intergenic | 15.0% |
| !! | AGAATAAGATTGATTATTGT+AGG | - | 32591:793-812 | MS.gene58510:CDS | 20.0% |
| !! | ATTATTATTTGCATGGTTAT+TGG | - | 32591:1291-1310 | MS.gene58510:CDS | 20.0% |
| !! | TTATTATTTGCATGGTTATT+GGG | - | 32591:1292-1311 | MS.gene58510:CDS | 20.0% |
| ! | GAGAATCATTATTATTTGCA+TGG | - | 32591:1284-1303 | MS.gene58510:CDS | 25.0% |
| ! | TATTATTTGCATGGTTATTG+GGG | - | 32591:1293-1312 | MS.gene58510:CDS | 25.0% |
| ! | TGATTTATGATGAGAAGAAT+TGG | - | 32591:1036-1055 | MS.gene58510:CDS | 25.0% |
| !! | GATTGAGATTTTCTATAACA+TGG | - | 32591:824-843 | MS.gene58510:CDS | 25.0% |
| AACAAATAATGATCACCAAC+AGG | + | 32591:762-781 | MS.gene58510:intergenic | 30.0% | |
| AACAATAGCTTCCTAATCAA+AGG | + | 32591:891-910 | MS.gene58510:intergenic | 30.0% | |
| AATCAGATATTGCAGATGTA+TGG | - | 32591:1488-1507 | MS.gene58510:CDS | 30.0% | |
| ACTTGATATTCTTGATGCTT+GGG | - | 32591:1106-1125 | MS.gene58510:CDS | 30.0% | |
| AGTTCATCTTTAACATCAAG+AGG | + | 32591:1575-1594 | MS.gene58510:intergenic | 30.0% | |
| ATCTGCTATGGTTTATTTGT+TGG | - | 32591:1223-1242 | MS.gene58510:CDS | 30.0% | |
| ATGATTGAGAATTATCATCC+TGG | - | 32591:1341-1360 | MS.gene58510:CDS | 30.0% | |
| ATTATTTGCATGGTTATTGG+GGG | - | 32591:1294-1313 | MS.gene58510:CDS | 30.0% | |
| ATTGATTATTGTAGGCTTCA+TGG | - | 32591:801-820 | MS.gene58510:CDS | 30.0% | |
| CTTGATGTTAAAGATGAACT+TGG | - | 32591:1575-1594 | MS.gene58510:CDS | 30.0% | |
| TATGATGAGAAGAATTGGAT+TGG | - | 32591:1041-1060 | MS.gene58510:CDS | 30.0% | |
| TGATGCTATGTTTACTGATA+TGG | - | 32591:950-969 | MS.gene58510:CDS | 30.0% | |
| TTGATTATTGTAGGCTTCAT+GGG | - | 32591:802-821 | MS.gene58510:CDS | 30.0% | |
| ! | ATAATTTTGTGATGCATGGT+TGG | - | 32591:1006-1025 | MS.gene58510:CDS | 30.0% |
| ! | ATGGATCGTGCTTATAATTT+TGG | - | 32591:1461-1480 | MS.gene58510:CDS | 30.0% |
| ! | GATCATAATTTTGTGATGCA+TGG | - | 32591:1002-1021 | MS.gene58510:CDS | 30.0% |
| !! | AATTGGATTGGTTTGAATAC+TGG | - | 32591:1053-1072 | MS.gene58510:CDS | 30.0% |
| !!! | ATACTGGAAGTTTTTTGTTG+AGG | - | 32591:1069-1088 | MS.gene58510:CDS | 30.0% |
| AAAAGAACACGTGGCTTATT+AGG | + | 32591:705-724 | MS.gene58509:intergenic | 35.0% | |
| ATTGAAATTCTTCGAGAACG+CGG | + | 32591:488-507 | MS.gene58510:intergenic | 35.0% | |
| CACTTGATATTCTTGATGCT+TGG | - | 32591:1105-1124 | MS.gene58510:CDS | 35.0% | |
| TAATCACCAAATTTCCCACA+AGG | + | 32591:1416-1435 | MS.gene58510:intergenic | 35.0% | |
| TGTAAACCTTGTGGGAAATT+TGG | - | 32591:1407-1426 | MS.gene58510:CDS | 35.0% | |
| ! | ATCCTGAAGTTGAGTTTTTG+TGG | - | 32591:916-935 | MS.gene58510:CDS | 35.0% |
| ! | ATGGTTTATTTGTTGGCAAC+TGG | - | 32591:1230-1249 | MS.gene58510:CDS | 35.0% |
| ! | GAGAATTATCATCCTGGTTT+TGG | - | 32591:1347-1366 | MS.gene58510:CDS | 35.0% |
| ! | TGGTTTATTTGTTGGCAACT+GGG | - | 32591:1231-1250 | MS.gene58510:CDS | 35.0% |
| ! | TTTTGTTGAGGAATTGTCAG+TGG | - | 32591:1081-1100 | MS.gene58510:CDS | 35.0% |
| !! | AACTTGATAGCTTTGAAAGC+AGG | + | 32591:1611-1630 | MS.gene58510:intergenic | 35.0% |
| !! | TGAGAGGTGTTTGAAACAAA+TGG | - | 32591:1442-1461 | MS.gene58510:CDS | 35.0% |
| !!! | ATTTTGGGACCAAGTGTATA+AGG | + | 32591:615-634 | MS.gene58510:intergenic | 35.0% |
| !!! | GTTATCGTTGTTGTTTTCTG+TGG | + | 32591:511-530 | MS.gene58510:intergenic | 35.0% |
| AAACCGTGTGAAAATCCTGT+TGG | - | 32591:744-763 | MS.gene58510:CDS | 40.0% | |
| AAGCTGGGAAGATTCTTACT+AGG | - | 32591:1162-1181 | MS.gene58510:CDS | 40.0% | |
| AGCAGATGATCAATCTGCTA+TGG | - | 32591:1211-1230 | MS.gene58510:CDS | 40.0% | |
| AGCTGGGAAGATTCTTACTA+GGG | - | 32591:1163-1182 | MS.gene58510:CDS | 40.0% | |
| ATCCTCAAAGATGAAGAAGC+TGG | - | 32591:561-580 | MS.gene58510:CDS | 40.0% | |
| CACCACAAAAACTCAACTTC+AGG | + | 32591:921-940 | MS.gene58510:intergenic | 40.0% | |
| CCAGTAACTAAAAGAACACG+TGG | + | 32591:714-733 | MS.gene58510:intergenic | 40.0% | |
| GATTGTCGAGATATCGAAAG+TGG | + | 32591:544-563 | MS.gene58510:intergenic | 40.0% | |
| GGCTTTACTTGATGCTGAAA+TGG | - | 32591:845-864 | MS.gene58510:CDS | 40.0% | |
| GTAGAAGAGTGAAGAGAGTT+AGG | - | 32591:1534-1553 | MS.gene58510:CDS | 40.0% | |
| GTTTACTGATATGGCTTTCG+AGG | - | 32591:959-978 | MS.gene58510:CDS | 40.0% | |
| GTTTCAAACACCTCTCAACA+GGG | + | 32591:1439-1458 | MS.gene58510:intergenic | 40.0% | |
| TAGGGAGTTAAAGAATCGAC+CGG | - | 32591:1181-1200 | MS.gene58510:CDS | 40.0% | |
| TCATCTCTAACCTTCCCTTT+AGG | + | 32591:1143-1162 | MS.gene58510:intergenic | 40.0% | |
| TGAACGTTGTTCATCCCAAT+CGG | + | 32591:640-659 | MS.gene58510:intergenic | 40.0% | |
| TGATCATCTGCTTCGAAAAC+CGG | + | 32591:1203-1222 | MS.gene58510:intergenic | 40.0% | |
| TGTTTCAAACACCTCTCAAC+AGG | + | 32591:1440-1459 | MS.gene58510:intergenic | 40.0% | |
| TTACTTGATGCTGAAATGGC+TGG | - | 32591:849-868 | MS.gene58510:CDS | 40.0% | |
| ! | ACAGGATTTTCACACGGTTT+AGG | + | 32591:744-763 | MS.gene58510:intergenic | 40.0% |
| ! | CTGAAGTTGAGTTTTTGTGG+TGG | - | 32591:919-938 | MS.gene58510:CDS | 40.0% |
| ! | TCACCAACAGGATTTTCACA+CGG | + | 32591:750-769 | MS.gene58510:intergenic | 40.0% |
| ! | TCATCCCAATCGGAGATTTT+GGG | + | 32591:630-649 | MS.gene58510:intergenic | 40.0% |
| ! | TTATTTGTTGGCAACTGGGA+GGG | - | 32591:1235-1254 | MS.gene58510:CDS | 40.0% |
| ! | TTCATCCCAATCGGAGATTT+TGG | + | 32591:631-650 | MS.gene58510:intergenic | 40.0% |
| ! | TTTATTTGTTGGCAACTGGG+AGG | - | 32591:1234-1253 | MS.gene58510:CDS | 40.0% |
| !! | AGTTGAGTTTTTGTGGTGGA+TGG | - | 32591:923-942 | MS.gene58510:CDS | 40.0% |
| !! | CATTGGTGACTCATTTTGTG+GGG | - | 32591:1384-1403 | MS.gene58510:CDS | 40.0% |
| !! | CCACGTGTTCTTTTAGTTAC+TGG | - | 32591:711-730 | MS.gene58510:CDS | 40.0% |
| !! | CCATTGGTGACTCATTTTGT+GGG | - | 32591:1383-1402 | MS.gene58510:CDS | 40.0% |
| !!! | ATCCTGGTTTTGGTGATCAT+AGG | - | 32591:1357-1376 | MS.gene58510:CDS | 40.0% |
| !!! | ATGCTGAAATGGCTGGTTTT+TGG | - | 32591:856-875 | MS.gene58510:CDS | 40.0% |
| !!! | TGCTGAAATGGCTGGTTTTT+GGG | - | 32591:857-876 | MS.gene58510:CDS | 40.0% |
| CACCAGCTTCTTCATCTTTG+AGG | + | 32591:566-585 | MS.gene58510:intergenic | 45.0% | |
| CACCTATGATCACCAAAACC+AGG | + | 32591:1362-1381 | MS.gene58510:intergenic | 45.0% | |
| CCAAACACGCCTTATACACT+TGG | - | 32591:603-622 | MS.gene58510:CDS | 45.0% | |
| CCAAGTGTATAAGGCGTGTT+TGG | + | 32591:606-625 | MS.gene58510:intergenic | 45.0% | |
| CCCACAAAATGAGTCACCAA+TGG | + | 32591:1386-1405 | MS.gene58510:intergenic | 45.0% | |
| GGAAGGTTAGAGATGAAGCT+GGG | - | 32591:1147-1166 | MS.gene58510:CDS | 45.0% | |
| GGGCTAAGCTTCCTTTGATT+AGG | - | 32591:877-896 | MS.gene58510:CDS | 45.0% | |
| TGATCCTTATAACGCTCCCA+CGG | + | 32591:987-1006 | MS.gene58510:intergenic | 45.0% | |
| TGGTCCCAAAATCTCCGATT+GGG | - | 32591:623-642 | MS.gene58510:CDS | 45.0% | |
| TTGGTCCCAAAATCTCCGAT+TGG | - | 32591:622-641 | MS.gene58510:CDS | 45.0% | |
| TTGTGGGGTGTAAACCTTGT+GGG | - | 32591:1399-1418 | MS.gene58510:CDS | 45.0% | |
| TTTGTGGGGTGTAAACCTTG+TGG | - | 32591:1398-1417 | MS.gene58510:CDS | 45.0% | |
| ! | GCATGGTTATTGGGGGATTT+TGG | - | 32591:1301-1320 | MS.gene58510:CDS | 45.0% |
| ! | TTGGTGATTACCCTGTTGAG+AGG | - | 32591:1426-1445 | MS.gene58510:CDS | 45.0% |
| !! | ATCTCGTTGAGGTCTTGTTC+TGG | + | 32591:423-442 | MS.gene58510:intergenic | 45.0% |
| !! | GCCATTGGTGACTCATTTTG+TGG | - | 32591:1382-1401 | MS.gene58510:CDS | 45.0% |
| !! | TTCTGGAGTTCCGAATTTGC+CGG | + | 32591:406-425 | MS.gene58510:intergenic | 45.0% |
| !!! | CTGGTTTTGGTGATCATAGG+TGG | - | 32591:1360-1379 | MS.gene58510:CDS | 45.0% |
| AATTCTTCGAGAACGCGGTG+AGG | + | 32591:483-502 | MS.gene58510:intergenic | 50.0% | |
| ACCTTCCCTTTAGGTCCCAT+TGG | + | 32591:1134-1153 | MS.gene58510:intergenic | 50.0% | |
| ATATGGCTTTCGAGGTTCCG+TGG | - | 32591:967-986 | MS.gene58510:CDS | 50.0% | |
| CACGTGAGTAGAGCTGTTGA+CGG | + | 32591:446-465 | MS.gene58510:intergenic | 50.0% | |
| CTTGATGCTTGGGCTCCAAT+GGG | - | 32591:1116-1135 | MS.gene58510:CDS | 50.0% | |
| GGGAAGGTTAGAGATGAAGC+TGG | - | 32591:1146-1165 | MS.gene58510:CDS | 50.0% | |
| GGGATGAACAACGTTCATCG+TGG | - | 32591:643-662 | MS.gene58510:CDS | 50.0% | |
| TATGGCTTTCGAGGTTCCGT+GGG | - | 32591:968-987 | MS.gene58510:CDS | 50.0% | |
| TCCAATGGGACCTAAAGGGA+AGG | - | 32591:1130-1149 | MS.gene58510:CDS | 50.0% | |
| TCTTGATGCTTGGGCTCCAA+TGG | - | 32591:1115-1134 | MS.gene58510:CDS | 50.0% | |
| TGGTGATCATAGGTGGCCAT+TGG | - | 32591:1367-1386 | MS.gene58510:CDS | 50.0% | |
| AACAGCTCTACTCACGTGGC+CGG | - | 32591:448-467 | MS.gene58510:CDS | 55.0% | |
| ATCGTCCTCCGTGGCACTAT+CGG | - | 32591:378-397 | MS.gene58510:CDS | 55.0% | |
| CGTCAACAGCTCTACTCACG+TGG | - | 32591:444-463 | MS.gene58510:CDS | 55.0% | |
| GCAACTGGGAGGGAACAATG+GGG | - | 32591:1245-1264 | MS.gene58510:CDS | 55.0% | |
| GGCAACTGGGAGGGAACAAT+GGG | - | 32591:1244-1263 | MS.gene58510:CDS | 55.0% | |
| GGGCTCCAATGGGACCTAAA+GGG | - | 32591:1126-1145 | MS.gene58510:CDS | 55.0% | |
| GGTGAGGAAGAGACAGAGGA+AGG | + | 32591:355-374 | MS.gene58510:intergenic | 55.0% | |
| TGACGGTGAGGAAGAGACAG+AGG | + | 32591:359-378 | MS.gene58510:intergenic | 55.0% | |
| TGGCAACTGGGAGGGAACAA+TGG | - | 32591:1243-1262 | MS.gene58510:CDS | 55.0% | |
| TGGGCTCCAATGGGACCTAA+AGG | - | 32591:1125-1144 | MS.gene58510:CDS | 55.0% | |
| ! | GCTGTTGACGGATCTCGTTG+AGG | + | 32591:434-453 | MS.gene58510:intergenic | 55.0% |
| !! | ACTATCGGCGCCGGCAAATT+CGG | - | 32591:393-412 | MS.gene58510:CDS | 55.0% |
| !! | GGTTCCGTGGGAGCGTTATA+AGG | - | 32591:980-999 | MS.gene58510:CDS | 55.0% |
| ACTGGGAGGGAACAATGGGG+TGG | - | 32591:1248-1267 | MS.gene58510:CDS | 60.0% | |
| AGTGCCACGGAGGACGATGA+CGG | + | 32591:376-395 | MS.gene58510:intergenic | 60.0% | |
| CTGGGAGGGAACAATGGGGT+GGG | - | 32591:1249-1268 | MS.gene58510:CDS | 60.0% | |
| GGAATGCGCGCTGCATCTGA+CGG | + | 32591:317-336 | MS.gene58510:intergenic | 60.0% | |
| !! | AGAGGAAGGTGAGAGTGCCG+CGG | + | 32591:341-360 | MS.gene58510:intergenic | 60.0% |
| CGCGCTGCATCTGACGGAGA+CGG | + | 32591:311-330 | MS.gene58510:intergenic | 65.0% | |
| CTCACCGTCATCGTCCTCCG+TGG | - | 32591:369-388 | MS.gene58510:CDS | 65.0% | |
| ! | CACGGAGGACGATGACGGTG+AGG | + | 32591:371-390 | MS.gene58510:intergenic | 65.0% |
| ATGCAGCGCGCATTCCGCCG+CGG | - | 32591:321-340 | MS.gene58510:CDS | 70.0% | |
| CTCCGTGGCACTATCGGCGC+CGG | - | 32591:384-403 | MS.gene58510:CDS | 70.0% | |
| !! | GGAAGGTGAGAGTGCCGCGG+CGG | + | 32591:338-357 | MS.gene58510:intergenic | 70.0% |
| !! | TGCCGGCGCCGATAGTGCCA+CGG | + | 32591:389-408 | MS.gene58510:intergenic | 70.0% |
| CGCGGTGAGGCTCAACGCGC+CGG | + | 32591:470-489 | MS.gene58510:intergenic | 75.0% | |
| !! | CGGCGCCGATAGTGCCACGG+AGG | + | 32591:386-405 | MS.gene58510:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32591 | gene | 282 | 1646 | 282 | ID=MS.gene58510 |
| 32591 | mRNA | 282 | 1646 | 282 | ID=MS.gene58510.t1;Parent=MS.gene58510 |
| 32591 | exon | 282 | 1646 | 282 | ID=MS.gene58510.t1.exon1;Parent=MS.gene58510.t1 |
| 32591 | CDS | 282 | 1646 | 282 | ID=cds.MS.gene58510.t1;Parent=MS.gene58510.t1 |
| Gene Sequence |
| Protein sequence |
