Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59128.t1 | XP_024631310.1 | 52.1 | 236 | 46 | 5 | 3 | 176 | 18 | 248 | 1.00E-56 | 229.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59128.t1 | A0A396JKW1 | 51.5 | 237 | 44 | 6 | 6 | 176 | 17 | 248 | 2.2e-53 | 218.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051941 | MS.gene59128 | 0.801192 | 1.01E-48 | -1.69E-46 |
| MS.gene055911 | MS.gene59128 | 0.800987 | 1.11E-48 | -1.69E-46 |
| MS.gene056514 | MS.gene59128 | 0.801476 | 8.84E-49 | -1.69E-46 |
| MS.gene057019 | MS.gene59128 | 0.81124 | 7.73E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59128.t1 | MTR_1g031560 | 47.500 | 240 | 54 | 6 | 3 | 174 | 18 | 253 | 4.53e-56 | 188 |
| MS.gene59128.t1 | MTR_1g031450 | 46.491 | 228 | 64 | 5 | 1 | 174 | 16 | 239 | 7.67e-54 | 178 |
| MS.gene59128.t1 | MTR_1g031520 | 66.187 | 139 | 40 | 3 | 41 | 176 | 117 | 251 | 7.29e-53 | 179 |
| MS.gene59128.t1 | MTR_1g031535 | 45.815 | 227 | 64 | 6 | 7 | 179 | 22 | 243 | 9.93e-52 | 167 |
| MS.gene59128.t1 | MTR_1g031490 | 43.644 | 236 | 58 | 9 | 3 | 171 | 21 | 248 | 2.60e-47 | 156 |
| MS.gene59128.t1 | MTR_1g031510 | 43.966 | 232 | 62 | 10 | 3 | 170 | 22 | 249 | 7.13e-42 | 149 |
| MS.gene59128.t1 | MTR_1g031400 | 61.818 | 110 | 37 | 2 | 67 | 174 | 1 | 107 | 2.53e-37 | 130 |
| MS.gene59128.t1 | MTR_1g031280 | 43.137 | 153 | 73 | 5 | 34 | 175 | 111 | 260 | 5.05e-24 | 99.4 |
| MS.gene59128.t1 | MTR_1g031280 | 83.333 | 36 | 5 | 1 | 5 | 40 | 24 | 58 | 1.79e-13 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAATGGTCTTTGTAAAAT+TGG | 0.114126 | 1.4:-15830533 | MS.gene59128:CDS |
| TGACAAATCTCTTTCAGATT+TGG | 0.245176 | 1.4:-15830678 | MS.gene59128:CDS |
| TTTATGGATTTGAACTTTCT+TGG | 0.256489 | 1.4:-15830556 | MS.gene59128:CDS |
| CAGAATTCTTTCTATGTTGA+TGG | 0.268570 | 1.4:-15830704 | MS.gene59128:CDS |
| ACATCATGGCCTCTTGAATA+TGG | 0.275292 | 1.4:-15830617 | MS.gene59128:CDS |
| CTCTTGTCACCACATTCTTC+TGG | 0.284196 | 1.4:+15831076 | None:intergenic |
| TAAGAATATTCTGTTCGATT+TGG | 0.285653 | 1.4:+15830776 | None:intergenic |
| CCTTTCTGTGCACATATTCC+AGG | 0.301155 | 1.4:-15830479 | MS.gene59128:intron |
| GACAAATCTCTTTCAGATTT+GGG | 0.318981 | 1.4:-15830677 | MS.gene59128:CDS |
| CTGATTTCTGTATTGATCAT+AGG | 0.339669 | 1.4:+15830841 | None:intergenic |
| GAATATTCTTATATTGATAA+AGG | 0.348807 | 1.4:-15830764 | MS.gene59128:CDS |
| TCTCTTTCAGATTTGGGACT+TGG | 0.355277 | 1.4:-15830671 | MS.gene59128:CDS |
| TTTGAACTTTCTTGGCTCAA+TGG | 0.379007 | 1.4:-15830548 | MS.gene59128:CDS |
| CATCAACATAGAAAGAATTC+TGG | 0.385259 | 1.4:+15830705 | None:intergenic |
| CAGATTTGGGACTTGGAGAC+GGG | 0.442912 | 1.4:-15830664 | MS.gene59128:CDS |
| CTATGATCAATACAGAAATC+AGG | 0.451527 | 1.4:-15830840 | MS.gene59128:CDS |
| TACAGAAATCAGGCTGATCA+TGG | 0.478206 | 1.4:-15830830 | MS.gene59128:CDS |
| TCAGATTTGGGACTTGGAGA+CGG | 0.489585 | 1.4:-15830665 | MS.gene59128:CDS |
| AATTTCAGCAGCTCTTACTA+CGG | 0.499357 | 1.4:-15830878 | MS.gene59128:intron |
| GAATATGTGCACAGAAAGGC+TGG | 0.503141 | 1.4:+15830483 | None:intergenic |
| ATAGATATCTTGATTCGCTT+AGG | 0.503320 | 1.4:-15830297 | MS.gene59128:CDS |
| GTATTGATCATAGGCTAGGT+AGG | 0.512248 | 1.4:+15830850 | None:intergenic |
| TAATGAGCAACAAAGTGTAC+GGG | 0.532478 | 1.4:-15831177 | MS.gene59128:CDS |
| CTAATGAGCAACAAAGTGTA+CGG | 0.539704 | 1.4:-15831178 | MS.gene59128:CDS |
| TATTCTTATATTGATAAAGG+TGG | 0.562233 | 1.4:-15830761 | MS.gene59128:CDS |
| TTCTGTATTGATCATAGGCT+AGG | 0.570771 | 1.4:+15830846 | None:intergenic |
| AATCTATGTATCTCACATCA+TGG | 0.577666 | 1.4:-15830631 | MS.gene59128:CDS |
| CATTGACTTAAAACAGTCTG+AGG | 0.578324 | 1.4:+15830269 | None:intergenic |
| TTGTACAGACATCCGTCGAA+TGG | 0.581724 | 1.4:-15830582 | MS.gene59128:CDS |
| ATCCATAAAATACCATTCGA+CGG | 0.588140 | 1.4:+15830570 | None:intergenic |
| ATGTTGTTTCCATATTCAAG+AGG | 0.588942 | 1.4:+15830608 | None:intergenic |
| TATTGATCATAGGCTAGGTA+GGG | 0.590909 | 1.4:+15830851 | None:intergenic |
| CCTGGAATATGTGCACAGAA+AGG | 0.590987 | 1.4:+15830479 | None:intergenic |
| AACAAGAGTCATGTTCAAGA+AGG | 0.596613 | 1.4:-15831147 | MS.gene59128:CDS |
| AAGAGTCATGTTCAAGAAGG+TGG | 0.600098 | 1.4:-15831144 | MS.gene59128:CDS |
| TGCAGGCATGTCTACTTCGG+TGG | 0.615375 | 1.4:-15830322 | MS.gene59128:intron |
| CAGAAGAATGTGGTGACAAG+AGG | 0.621774 | 1.4:-15831075 | MS.gene59128:intron |
| TGTTGCAGGCATGTCTACTT+CGG | 0.634874 | 1.4:-15830325 | MS.gene59128:intron |
| TCATGTTCAAGAAGGTGGTG+CGG | 0.678993 | 1.4:-15831139 | MS.gene59128:CDS |
| AAAGACAGTCCAGAAGAATG+TGG | 0.689791 | 1.4:-15831085 | MS.gene59128:CDS |
| AATGAGCAACAAAGTGTACG+GGG | 0.756172 | 1.4:-15831176 | MS.gene59128:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTTATTATTATTTTCT+GGG | + | chr1.4:15830992-15831011 | None:intergenic | 10.0% |
| !!! | TGAAATTTATTATTATTTTC+TGG | + | chr1.4:15830993-15831012 | None:intergenic | 10.0% |
| !! | GAATATTCTTATATTGATAA+AGG | - | chr1.4:15830668-15830687 | MS.gene59128:CDS | 15.0% |
| !! | ATTCATAGTACAAAATCAAT+TGG | + | chr1.4:15830409-15830428 | None:intergenic | 20.0% |
| !! | TATTCTTATATTGATAAAGG+TGG | - | chr1.4:15830671-15830690 | MS.gene59128:CDS | 20.0% |
| ! | ATTGAAATCCAATAATCGTA+TGG | + | chr1.4:15830486-15830505 | None:intergenic | 25.0% |
| ! | TAAGAATATTCTGTTCGATT+TGG | + | chr1.4:15830659-15830678 | None:intergenic | 25.0% |
| ! | TATATCGATAATAGACGATT+TGG | - | chr1.4:15831053-15831072 | MS.gene59128:intron | 25.0% |
| ! | TATGAATTTGAAGAATACCA+TGG | - | chr1.4:15830422-15830441 | MS.gene59128:intron | 25.0% |
| ! | TTTATGGATTTGAACTTTCT+TGG | - | chr1.4:15830876-15830895 | MS.gene59128:intron | 25.0% |
| !! | ATTATTATTTTCTGGGTGTT+TGG | + | chr1.4:15830985-15831004 | None:intergenic | 25.0% |
| !! | ATTGTACATAGTATTTTCCA+TGG | + | chr1.4:15830442-15830461 | None:intergenic | 25.0% |
| AATCTATGTATCTCACATCA+TGG | - | chr1.4:15830801-15830820 | MS.gene59128:CDS | 30.0% | |
| ATCCATAAAATACCATTCGA+CGG | + | chr1.4:15830865-15830884 | None:intergenic | 30.0% | |
| CAGAATTCTTTCTATGTTGA+TGG | - | chr1.4:15830728-15830747 | MS.gene59128:CDS | 30.0% | |
| CATCAACATAGAAAGAATTC+TGG | + | chr1.4:15830730-15830749 | None:intergenic | 30.0% | |
| CTATGATCAATACAGAAATC+AGG | - | chr1.4:15830592-15830611 | MS.gene59128:CDS | 30.0% | |
| CTCAATGGTCTTTGTAAAAT+TGG | - | chr1.4:15830899-15830918 | MS.gene59128:intron | 30.0% | |
| GACAAATCTCTTTCAGATTT+GGG | - | chr1.4:15830755-15830774 | MS.gene59128:CDS | 30.0% | |
| TATATAGACCCATTTCCAAA+TGG | + | chr1.4:15831027-15831046 | None:intergenic | 30.0% | |
| TGACAAATCTCTTTCAGATT+TGG | - | chr1.4:15830754-15830773 | MS.gene59128:CDS | 30.0% | |
| TTATAGAGTCCCAAAGAATA+AGG | + | chr1.4:15830536-15830555 | None:intergenic | 30.0% | |
| TTCATATGGATTCTACCATT+TGG | - | chr1.4:15831009-15831028 | MS.gene59128:intron | 30.0% | |
| ! | ATAGATATCTTGATTCGCTT+AGG | - | chr1.4:15831135-15831154 | MS.gene59128:CDS | 30.0% |
| ! | CTGATTTCTGTATTGATCAT+AGG | + | chr1.4:15830594-15830613 | None:intergenic | 30.0% |
| !! | ATGTTGTTTCCATATTCAAG+AGG | + | chr1.4:15830827-15830846 | None:intergenic | 30.0% |
| AACAAGAGTCATGTTCAAGA+AGG | - | chr1.4:15830285-15830304 | MS.gene59128:CDS | 35.0% | |
| AATTTCAGCAGCTCTTACTA+CGG | - | chr1.4:15830554-15830573 | MS.gene59128:CDS | 35.0% | |
| AGTCCCAAAGAATAAGGATA+AGG | + | chr1.4:15830530-15830549 | None:intergenic | 35.0% | |
| CAACTTCACCATACGATTAT+TGG | - | chr1.4:15830475-15830494 | MS.gene59128:intron | 35.0% | |
| GGATTCTACCATTTGGAAAT+GGG | - | chr1.4:15831016-15831035 | MS.gene59128:intron | 35.0% | |
| GTCCCAAAGAATAAGGATAA+GGG | + | chr1.4:15830529-15830548 | None:intergenic | 35.0% | |
| TATTGATCATAGGCTAGGTA+GGG | + | chr1.4:15830584-15830603 | None:intergenic | 35.0% | |
| TCCCAAAGAATAAGGATAAG+GGG | + | chr1.4:15830528-15830547 | None:intergenic | 35.0% | |
| TGGATTCTACCATTTGGAAA+TGG | - | chr1.4:15831015-15831034 | MS.gene59128:intron | 35.0% | |
| TTTGAACTTTCTTGGCTCAA+TGG | - | chr1.4:15830884-15830903 | MS.gene59128:intron | 35.0% | |
| ! | ATAACATTAGCCCTCCTTTT+AGG | - | chr1.4:15830321-15830340 | MS.gene59128:intron | 35.0% |
| ! | ATCCGTCGAATGGTATTTTA+TGG | - | chr1.4:15830860-15830879 | MS.gene59128:CDS | 35.0% |
| ! | ATGTTTTGTGTGTATGTTGC+AGG | - | chr1.4:15831093-15831112 | MS.gene59128:CDS | 35.0% |
| ! | TGTCTTTTAGCCTAAAAGGA+GGG | + | chr1.4:15830334-15830353 | None:intergenic | 35.0% |
| ! | TTCTGTATTGATCATAGGCT+AGG | + | chr1.4:15830589-15830608 | None:intergenic | 35.0% |
| !! | CATTGACTTAAAACAGTCTG+AGG | + | chr1.4:15831166-15831185 | None:intergenic | 35.0% |
| AAAGACAGTCCAGAAGAATG+TGG | - | chr1.4:15830347-15830366 | MS.gene59128:intron | 40.0% | |
| AAGAGTCATGTTCAAGAAGG+TGG | - | chr1.4:15830288-15830307 | MS.gene59128:CDS | 40.0% | |
| ACATCATGGCCTCTTGAATA+TGG | - | chr1.4:15830815-15830834 | MS.gene59128:CDS | 40.0% | |
| ACCCCCTTATCCTTATTCTT+TGG | - | chr1.4:15830523-15830542 | MS.gene59128:CDS | 40.0% | |
| CCCAAAGAATAAGGATAAGG+GGG | + | chr1.4:15830527-15830546 | None:intergenic | 40.0% | |
| CCCCCTTATCCTTATTCTTT+GGG | - | chr1.4:15830524-15830543 | MS.gene59128:CDS | 40.0% | |
| GGTTATTGTCCTCACATGAT+AGG | + | chr1.4:15830388-15830407 | None:intergenic | 40.0% | |
| TACAGAAATCAGGCTGATCA+TGG | - | chr1.4:15830602-15830621 | MS.gene59128:CDS | 40.0% | |
| ! | CTGTCTTTTAGCCTAAAAGG+AGG | + | chr1.4:15830335-15830354 | None:intergenic | 40.0% |
| ! | GGACTGTCTTTTAGCCTAAA+AGG | + | chr1.4:15830338-15830357 | None:intergenic | 40.0% |
| ! | GTATTGATCATAGGCTAGGT+AGG | + | chr1.4:15830585-15830604 | None:intergenic | 40.0% |
| ! | TCTCTTTCAGATTTGGGACT+TGG | - | chr1.4:15830761-15830780 | MS.gene59128:CDS | 40.0% |
| CAGAAGAATGTGGTGACAAG+AGG | - | chr1.4:15830357-15830376 | MS.gene59128:intron | 45.0% | |
| CCTGGAATATGTGCACAGAA+AGG | + | chr1.4:15830956-15830975 | None:intergenic | 45.0% | |
| CCTTTCTGTGCACATATTCC+AGG | - | chr1.4:15830953-15830972 | MS.gene59128:intron | 45.0% | |
| CTCTTGTCACCACATTCTTC+TGG | + | chr1.4:15830359-15830378 | None:intergenic | 45.0% | |
| GAATATGTGCACAGAAAGGC+TGG | + | chr1.4:15830952-15830971 | None:intergenic | 45.0% | |
| GAGGTACAACCTATCATGTG+AGG | - | chr1.4:15830376-15830395 | MS.gene59128:intron | 45.0% | |
| TCATGTTCAAGAAGGTGGTG+CGG | - | chr1.4:15830293-15830312 | MS.gene59128:CDS | 45.0% | |
| TGTTGCAGGCATGTCTACTT+CGG | - | chr1.4:15831107-15831126 | MS.gene59128:CDS | 45.0% | |
| TTGTACAGACATCCGTCGAA+TGG | - | chr1.4:15830850-15830869 | MS.gene59128:CDS | 45.0% | |
| ! | TCAGATTTGGGACTTGGAGA+CGG | - | chr1.4:15830767-15830786 | MS.gene59128:CDS | 45.0% |
| !! | AAATAATAATAAATTTCATA+TGG | - | chr1.4:15830995-15831014 | MS.gene59128:intron | 5.0% |
| ! | CAGATTTGGGACTTGGAGAC+GGG | - | chr1.4:15830768-15830787 | MS.gene59128:CDS | 50.0% |
| TGCAGGCATGTCTACTTCGG+TGG | - | chr1.4:15831110-15831129 | MS.gene59128:CDS | 55.0% | |
| ! | CTGGGTGTTTGGAGCAAACC+TGG | + | chr1.4:15830974-15830993 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 15830257 | 15831197 | 15830257 | ID=MS.gene59128 |
| chr1.4 | mRNA | 15830257 | 15831197 | 15830257 | ID=MS.gene59128.t1;Parent=MS.gene59128 |
| chr1.4 | exon | 15831076 | 15831197 | 15831076 | ID=MS.gene59128.t1.exon1;Parent=MS.gene59128.t1 |
| chr1.4 | CDS | 15831076 | 15831197 | 15831076 | ID=cds.MS.gene59128.t1;Parent=MS.gene59128.t1 |
| chr1.4 | exon | 15830480 | 15830892 | 15830480 | ID=MS.gene59128.t1.exon2;Parent=MS.gene59128.t1 |
| chr1.4 | CDS | 15830480 | 15830892 | 15830480 | ID=cds.MS.gene59128.t1;Parent=MS.gene59128.t1 |
| chr1.4 | exon | 15830257 | 15830339 | 15830257 | ID=MS.gene59128.t1.exon3;Parent=MS.gene59128.t1 |
| chr1.4 | CDS | 15830257 | 15830339 | 15830257 | ID=cds.MS.gene59128.t1;Parent=MS.gene59128.t1 |
| Gene Sequence |
| Protein sequence |
