AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59574


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59574.t1 MTR_8g027860 97.701 87 2 0 1 87 70 156 3.54e-58 181
MS.gene59574.t1 MTR_7g117200 63.158 76 24 3 16 87 63 138 1.41e-22 89.0
MS.gene59574.t1 MTR_8g099350 57.143 70 26 2 22 87 83 152 1.05e-18 78.6
MS.gene59574.t1 MTR_3g090860 50.000 76 36 2 14 87 78 153 1.37e-17 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59574.t1 AT4G11070 55.882 68 24 2 24 87 64 129 1.71e-16 72.0
MS.gene59574.t1 AT4G11070 55.882 68 24 2 24 87 96 161 1.99e-16 72.0
MS.gene59574.t1 AT4G23810 50.000 74 28 2 23 87 105 178 8.54e-16 70.5
MS.gene59574.t1 AT5G24110 48.438 64 31 1 24 87 72 133 1.52e-15 69.7
MS.gene59574.t1 AT2G46400 60.784 51 19 1 38 87 74 124 2.93e-12 60.5

Find 24 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCAAGTGAAGGTTTATTC+TGG 0.340277 8.3:-65940450 MS.gene59574:CDS
TGTGCTTGAACTTTGAATCT+TGG 0.345830 8.3:+65940657 None:intergenic
GGTTATAGTTGGAGAAAGTA+TGG 0.368943 8.3:-65940399 MS.gene59574:CDS
TATGGACAAAAGGATATTCT+TGG 0.372931 8.3:-65940381 MS.gene59574:CDS
TGATTGTTACCTTGGAAACT+TGG 0.389226 8.3:+65940356 None:intergenic
ATGGATTCTCATTGTTCAAA+TGG 0.390219 8.3:-65940698 MS.gene59574:CDS
CTGTTGAGGATGGTTATAGT+TGG 0.443101 8.3:-65940410 MS.gene59574:CDS
TTGGAGAAAGTATGGACAAA+AGG 0.456392 8.3:-65940391 MS.gene59574:CDS
TCCCTCATTACCCTTTGCCA+TGG 0.480628 8.3:+65940748 None:intergenic
AACCATGGCAAAGGGTAATG+AGG 0.498135 8.3:-65940750 None:intergenic
ACAAACATGTGTCCAAGAAA+AGG 0.507877 8.3:-65940637 MS.gene59574:intron
GACAAACAATGTCAACATGA+TGG 0.527140 8.3:-65940717 MS.gene59574:CDS
TTCTTGGAGCCAAGTTTCCA+AGG 0.549044 8.3:-65940365 MS.gene59574:CDS
TTCACTTGCTCTGTCCACTT+AGG 0.560248 8.3:+65940462 None:intergenic
GACAGCAATAACCATGGCAA+AGG 0.573478 8.3:-65940759 None:intergenic
ACAGCAATAACCATGGCAAA+GGG 0.573968 8.3:-65940758 None:intergenic
TATTCTGGAACAGAAGCTGA+AGG 0.581805 8.3:-65940435 MS.gene59574:CDS
ACCATGGCAAAGGGTAATGA+GGG 0.583479 8.3:-65940749 MS.gene59574:CDS
GCTGAAGGGTCTGTTGAGGA+TGG 0.595548 8.3:-65940420 MS.gene59574:CDS
TTGCTCTGTCCACTTAGGCA+CGG 0.602998 8.3:+65940467 None:intergenic
ATTCTGGAACAGAAGCTGAA+GGG 0.629611 8.3:-65940434 MS.gene59574:CDS
AAATGGAAGCCCTAAAAGTG+AGG 0.654127 8.3:-65940681 MS.gene59574:CDS
AGAAGCTGAAGGGTCTGTTG+AGG 0.659448 8.3:-65940424 MS.gene59574:CDS
TAAGTGGACAGAGCAAGTGA+AGG 0.673359 8.3:-65940460 MS.gene59574:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATAATTTTCATTATTCTT+TGG - chr8.3:65940598-65940617 MS.gene59574:intron 15.0%
!!! ATAGGAATTTTTTTTTTCCA+AGG - chr8.3:65940566-65940585 MS.gene59574:intron 20.0%
!!! TTCAAGGTTCATTTTTTTAT+AGG - chr8.3:65940548-65940567 MS.gene59574:intron 20.0%
!! TAATCCAATATACCTTTTCT+TGG + chr8.3:65940486-65940505 None:intergenic 25.0%
!!! CATTATTCTTTGGAATTTTG+TGG - chr8.3:65940608-65940627 MS.gene59574:intron 25.0%
ATGGATTCTCATTGTTCAAA+TGG - chr8.3:65940410-65940429 MS.gene59574:CDS 30.0%
TATGGACAAAAGGATATTCT+TGG - chr8.3:65940727-65940746 MS.gene59574:CDS 30.0%
!!! TCATTGATTCCTTTTGAACT+TGG - chr8.3:65940516-65940535 MS.gene59574:intron 30.0%
AATTATGAGATCGTGAACCT+TGG + chr8.3:65940586-65940605 None:intergenic 35.0%
AATTCACCACCAAGTTCAAA+AGG + chr8.3:65940528-65940547 None:intergenic 35.0%
ACAAACATGTGTCCAAGAAA+AGG - chr8.3:65940471-65940490 MS.gene59574:CDS 35.0%
GACAAACAATGTCAACATGA+TGG - chr8.3:65940391-65940410 MS.gene59574:CDS 35.0%
GGTTATAGTTGGAGAAAGTA+TGG - chr8.3:65940709-65940728 MS.gene59574:CDS 35.0%
TGTGCTTGAACTTTGAATCT+TGG + chr8.3:65940454-65940473 None:intergenic 35.0%
! GTGTCCAAGAAAAGGTATAT+TGG - chr8.3:65940479-65940498 MS.gene59574:intron 35.0%
! TTGATTCCTTTTGAACTTGG+TGG - chr8.3:65940519-65940538 MS.gene59574:intron 35.0%
! TTGGAGAAAGTATGGACAAA+AGG - chr8.3:65940717-65940736 MS.gene59574:CDS 35.0%
!! AATCTTGGACCTCACTTTTA+GGG + chr8.3:65940439-65940458 None:intergenic 35.0%
!!! AACTTGGTGGTGAATTTTCA+AGG - chr8.3:65940532-65940551 MS.gene59574:intron 35.0%
AAATGGAAGCCCTAAAAGTG+AGG - chr8.3:65940427-65940446 MS.gene59574:CDS 40.0%
CTGTTGAGGATGGTTATAGT+TGG - chr8.3:65940698-65940717 MS.gene59574:CDS 40.0%
GAGCAAGTGAAGGTTTATTC+TGG - chr8.3:65940658-65940677 MS.gene59574:CDS 40.0%
!! ATTCTGGAACAGAAGCTGAA+GGG - chr8.3:65940674-65940693 MS.gene59574:CDS 40.0%
!! GAATCTTGGACCTCACTTTT+AGG + chr8.3:65940440-65940459 None:intergenic 40.0%
!! TATTCTGGAACAGAAGCTGA+AGG - chr8.3:65940673-65940692 MS.gene59574:CDS 40.0%
TAAGTGGACAGAGCAAGTGA+AGG - chr8.3:65940648-65940667 MS.gene59574:CDS 45.0%
TTCACTTGCTCTGTCCACTT+AGG + chr8.3:65940649-65940668 None:intergenic 45.0%
TTCTTGGAGCCAAGTTTCCA+AGG - chr8.3:65940743-65940762 MS.gene59574:CDS 45.0%
AGAAGCTGAAGGGTCTGTTG+AGG - chr8.3:65940684-65940703 MS.gene59574:CDS 50.0%
GCAGAAAAACCGTGCCTAAG+TGG - chr8.3:65940632-65940651 MS.gene59574:intron 50.0%
TTGCTCTGTCCACTTAGGCA+CGG + chr8.3:65940644-65940663 None:intergenic 50.0%
GCTGAAGGGTCTGTTGAGGA+TGG - chr8.3:65940688-65940707 MS.gene59574:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 65940362 65940768 65940362 ID=MS.gene59574
chr8.3 mRNA 65940362 65940768 65940362 ID=MS.gene59574.t1;Parent=MS.gene59574
chr8.3 exon 65940638 65940768 65940638 ID=MS.gene59574.t1.exon1;Parent=MS.gene59574.t1
chr8.3 CDS 65940638 65940768 65940638 ID=cds.MS.gene59574.t1;Parent=MS.gene59574.t1
chr8.3 exon 65940362 65940494 65940362 ID=MS.gene59574.t1.exon2;Parent=MS.gene59574.t1
chr8.3 CDS 65940362 65940494 65940362 ID=cds.MS.gene59574.t1;Parent=MS.gene59574.t1
Gene Sequence

>MS.gene59574

ATGGCAAAGGGTAATGAGGGAAAAATTAAGACAAACAATGTCAACATGATGGATTCTCATTGTTCAAATGGAAGCCCTAAAAGTGAGGTCCAAGATTCAAAGTTCAAGCACAAACATGTGTCCAAGAAAAGGTATATTGGATTAAATTACAAAATCATTGATTCCTTTTGAACTTGGTGGTGAATTTTCAAGGTTCATTTTTTTATAGGAATTTTTTTTTTCCAAGGTTCACGATCTCATAATTTTCATTATTCTTTGGAATTTTGTGGTGCAGAAAAACCGTGCCTAAGTGGACAGAGCAAGTGAAGGTTTATTCTGGAACAGAAGCTGAAGGGTCTGTTGAGGATGGTTATAGTTGGAGAAAGTATGGACAAAAGGATATTCTTGGAGCCAAGTTTCCAAGGTAA

Protein sequence

>MS.gene59574.t1

MAKGNEGKIKTNNVNMMDSHCSNGSPKSEVQDSKFKHKHVSKKRKTVPKWTEQVKVYSGTEAEGSVEDGYSWRKYGQKDILGAKFPR