Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59627.t1 | XP_003611519.1 | 94.6 | 92 | 5 | 0 | 1 | 92 | 40 | 131 | 1.30E-46 | 195.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59627.t1 | Q9T068 | 55.3 | 85 | 31 | 2 | 15 | 92 | 44 | 128 | 1.0e-20 | 100.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59627.t1 | G7K151 | 94.6 | 92 | 5 | 0 | 1 | 92 | 40 | 131 | 9.1e-47 | 195.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058168 | MS.gene59627 | 0.8007 | 1.27E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAATTATTTTTCTCATTTG+AGG | - | 37983:15846-15865 | MS.gene59627:CDS | 15.0% |
| !! | AAATGTAAACAAAATTTACC+TGG | + | 37983:15778-15797 | MS.gene59627:intergenic | 20.0% |
| !! | AAGTTATAAGAATTTAGAAG+AGG | + | 37983:15817-15836 | MS.gene59627:intergenic | 20.0% |
| ATAAGAATTTAGAAGAGGCA+GGG | + | 37983:15812-15831 | MS.gene59627:intergenic | 30.0% | |
| CTAATTAAGATGAGTGATGT+AGG | - | 37983:15974-15993 | MS.gene59627:intron | 30.0% | |
| TATAAGAATTTAGAAGAGGC+AGG | + | 37983:15813-15832 | MS.gene59627:intergenic | 30.0% | |
| AATAGGTGATAACAAGGTGA+TGG | - | 37983:16028-16047 | MS.gene59627:intron | 35.0% | |
| AGAAAGACTTTGAACCAACA+TGG | - | 37983:15746-15765 | MS.gene59627:CDS | 35.0% | |
| CATTTGCATTTCCAACTCAT+AGG | + | 37983:16227-16246 | MS.gene59627:intergenic | 35.0% | |
| CCAACTATAAACCTATGAGT+TGG | - | 37983:16213-16232 | MS.gene59627:intron | 35.0% | |
| CCAACTCATAGGTTTATAGT+TGG | + | 37983:16216-16235 | MS.gene59627:intergenic | 35.0% | |
| ! | ATGAGTTGGAAATGCAAATG+TGG | - | 37983:16227-16246 | MS.gene59627:intron | 35.0% |
| ! | TGAGTTGGAAATGCAAATGT+GGG | - | 37983:16228-16247 | MS.gene59627:intron | 35.0% |
| !! | TGTTGATTTGTGCAGACAAT+AGG | - | 37983:16011-16030 | MS.gene59627:intron | 35.0% |
| AGACTGAGTAAACGAGTTGT+CGG | + | 37983:15951-15970 | MS.gene59627:intergenic | 40.0% | |
| GCAGACAATAGGTGATAACA+AGG | - | 37983:16022-16041 | MS.gene59627:intron | 40.0% | |
| GTAAGAGTTTGTCAACTCAG+TGG | + | 37983:15921-15940 | MS.gene59627:intergenic | 40.0% | |
| TTACCTGGTGAAAACCATGT+TGG | + | 37983:15763-15782 | MS.gene59627:intergenic | 40.0% | |
| ! | CACCTTGTATCACATTTTGG+TGG | + | 37983:16077-16096 | MS.gene59627:intergenic | 40.0% |
| ! | CTGCACCTTGTATCACATTT+TGG | + | 37983:16080-16099 | MS.gene59627:intergenic | 40.0% |
| ATCTTTCCTGTCATTGCTCG+AGG | + | 37983:16143-16162 | MS.gene59627:intergenic | 45.0% | |
| CAACTGCAGACAGAGTTGAA+GGG | + | 37983:16169-16188 | MS.gene59627:intergenic | 45.0% | |
| GATACAAGGTGCAGATTCTG+TGG | - | 37983:16086-16105 | MS.gene59627:intron | 45.0% | |
| GGCCACCAAAATGTGATACA+AGG | - | 37983:16072-16091 | MS.gene59627:intron | 45.0% | |
| TGAGGAAGCAGATAGGATCA+AGG | - | 37983:16051-16070 | MS.gene59627:intron | 45.0% | |
| TGTCTGCAGTTGCTTATGCT+AGG | - | 37983:16177-16196 | MS.gene59627:intron | 45.0% | |
| ! | GAACCAACATGGTTTTCACC+AGG | - | 37983:15757-15776 | MS.gene59627:CDS | 45.0% |
| ! | GTGATAACAAGGTGATGGTG+AGG | - | 37983:16033-16052 | MS.gene59627:intron | 45.0% |
| CTGCAGTTGCTTATGCTAGG+AGG | - | 37983:16180-16199 | MS.gene59627:intron | 50.0% | |
| GCAAATCCTCGAGCAATGAC+AGG | - | 37983:16134-16153 | MS.gene59627:intron | 50.0% | |
| GCAACTGCAGACAGAGTTGA+AGG | + | 37983:16170-16189 | MS.gene59627:intergenic | 50.0% | |
| GTCATTGCTCGAGGATTTGC+TGG | + | 37983:16134-16153 | MS.gene59627:intergenic | 50.0% | |
| ! | GTGATGGTGAGGAAGCAGAT+AGG | - | 37983:16044-16063 | MS.gene59627:intron | 50.0% |
| !! | TAAACGAGTTGTCGGCTCAG+TGG | + | 37983:15943-15962 | MS.gene59627:intergenic | 50.0% |
| CAGATTCTGTGGTCACTGCG+AGG | - | 37983:16097-16116 | MS.gene59627:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37983 | gene | 15744 | 16271 | 15744 | ID=MS.gene59627 |
| 37983 | mRNA | 15744 | 16271 | 15744 | ID=MS.gene59627.t1;Parent=MS.gene59627 |
| 37983 | exon | 16237 | 16271 | 16237 | ID=MS.gene59627.t1.exon1;Parent=MS.gene59627.t1 |
| 37983 | CDS | 16237 | 16271 | 16237 | ID=cds.MS.gene59627.t1;Parent=MS.gene59627.t1 |
| 37983 | exon | 15744 | 15989 | 15744 | ID=MS.gene59627.t1.exon2;Parent=MS.gene59627.t1 |
| 37983 | CDS | 15744 | 15989 | 15744 | ID=cds.MS.gene59627.t1;Parent=MS.gene59627.t1 |
| Gene Sequence |
| Protein sequence |
