Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59888.t1 | XP_003610296.2 | 86.9 | 153 | 17 | 2 | 1 | 150 | 38 | 190 | 1.90E-66 | 261.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59888.t1 | G7JFL0 | 86.9 | 153 | 17 | 2 | 1 | 150 | 1 | 153 | 1.3e-66 | 261.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056677 | MS.gene59888 | 0.802648 | 5.08E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59888.t1 | MTR_4g130640 | 86.928 | 153 | 17 | 2 | 1 | 150 | 1 | 153 | 5.53e-92 | 264 |
| MS.gene59888.t1 | MTR_7g069410 | 45.033 | 151 | 41 | 5 | 1 | 150 | 1 | 110 | 6.04e-28 | 100 |
| MS.gene59888.t1 | MTR_6g023775 | 41.237 | 97 | 23 | 2 | 80 | 142 | 65 | 161 | 2.00e-13 | 64.3 |
| MS.gene59888.t1 | MTR_7g031710 | 70.270 | 37 | 11 | 0 | 113 | 149 | 42 | 78 | 4.18e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTTGAAAAGAAATAAAA+TGG | 0.207796 | 8.3:-53285893 | MS.gene59888:CDS |
| GAAATAAAATGGATCGAAAA+AGG | 0.213477 | 8.3:-53285882 | MS.gene59888:CDS |
| CATCATGTTCTGAGAGAAAT+TGG | 0.219553 | 8.3:-53285936 | MS.gene59888:CDS |
| CTAGTAACACTTTCTTCTAT+TGG | 0.245408 | 8.3:+53286156 | None:intergenic |
| TAGTGGTTTACTATCATCTT+GGG | 0.287206 | 8.3:+53286113 | None:intergenic |
| TTTCACTTATTGAAAATTGC+TGG | 0.358067 | 8.3:-53285982 | MS.gene59888:CDS |
| AATTGCTGGTGAAGGAGTTC+AGG | 0.416502 | 8.3:-53285968 | MS.gene59888:CDS |
| TGAGTTATCTTTAGATGAAC+CGG | 0.418024 | 8.3:-53285806 | MS.gene59888:CDS |
| GCTACAATGTTCTTTGATGA+TGG | 0.418641 | 8.3:-53285775 | MS.gene59888:CDS |
| CTGCAGGTGGAAACAAGCTT+TGG | 0.435890 | 8.3:-53286056 | MS.gene59888:CDS |
| TAGAAGAAAGTGTTACTAGT+TGG | 0.440821 | 8.3:-53286152 | MS.gene59888:CDS |
| AACATTGTAGCCTCCAACTC+CGG | 0.456994 | 8.3:+53285787 | None:intergenic |
| ATAGTGGTTTACTATCATCT+TGG | 0.457884 | 8.3:+53286112 | None:intergenic |
| ATCTTTAGATGAACCGGAGT+TGG | 0.478188 | 8.3:-53285800 | MS.gene59888:CDS |
| GTAACACTTTCTTCTATTGG+AGG | 0.479958 | 8.3:+53286159 | None:intergenic |
| AAGATGATAGTAAACCACTA+TGG | 0.482539 | 8.3:-53286110 | MS.gene59888:CDS |
| TTTAGATGAACCGGAGTTGG+AGG | 0.500804 | 8.3:-53285797 | MS.gene59888:CDS |
| ATGTTCTTTGATGATGGAAA+CGG | 0.513119 | 8.3:-53285769 | MS.gene59888:CDS |
| GTGCATACTAATGTTGGAGT+TGG | 0.524300 | 8.3:-53285841 | MS.gene59888:CDS |
| GTTTATGTGCATACTAATGT+TGG | 0.532305 | 8.3:-53285847 | MS.gene59888:CDS |
| TCCTCTGAAGTAGTGAATGT+TGG | 0.537803 | 8.3:-53285718 | MS.gene59888:CDS |
| AGTGGTTTACTATCATCTTG+GGG | 0.567164 | 8.3:+53286114 | None:intergenic |
| AGAGGAAACACGGATTGTAT+CGG | 0.567785 | 8.3:+53285735 | None:intergenic |
| TTATTGAAAATTGCTGGTGA+AGG | 0.583272 | 8.3:-53285976 | MS.gene59888:CDS |
| TTCTTTGATGATGGAAACGG+AGG | 0.597380 | 8.3:-53285766 | MS.gene59888:CDS |
| GTGCAGAATAATGATTCTGC+AGG | 0.611185 | 8.3:-53286072 | MS.gene59888:CDS |
| CAGAATAATGATTCTGCAGG+TGG | 0.615421 | 8.3:-53286069 | MS.gene59888:CDS |
| TGATGATGGAAACGGAGGAG+AGG | 0.621097 | 8.3:-53285761 | MS.gene59888:CDS |
| TCCAACATTCACTACTTCAG+AGG | 0.626874 | 8.3:+53285717 | None:intergenic |
| TCACTACTTCAGAGGAAACA+CGG | 0.630269 | 8.3:+53285725 | None:intergenic |
| ACAGTGACAAATCTCCATAG+TGG | 0.653867 | 8.3:+53286096 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCTTTGAAAAGAAATAAAA+TGG | - | chr8.3:53285980-53285999 | MS.gene59888:CDS | 15.0% |
| !! | AAATAAAATGGATCGAAAAA+GGG | - | chr8.3:53285992-53286011 | MS.gene59888:CDS | 20.0% |
| ! | GAAATAAAATGGATCGAAAA+AGG | - | chr8.3:53285991-53286010 | MS.gene59888:CDS | 25.0% |
| ! | TTTCACTTATTGAAAATTGC+TGG | - | chr8.3:53285891-53285910 | MS.gene59888:CDS | 25.0% |
| AAGATGATAGTAAACCACTA+TGG | - | chr8.3:53285763-53285782 | MS.gene59888:CDS | 30.0% | |
| ATAGTGGTTTACTATCATCT+TGG | + | chr8.3:53285764-53285783 | None:intergenic | 30.0% | |
| CTAGTAACACTTTCTTCTAT+TGG | + | chr8.3:53285720-53285739 | None:intergenic | 30.0% | |
| GTTTATGTGCATACTAATGT+TGG | - | chr8.3:53286026-53286045 | MS.gene59888:CDS | 30.0% | |
| TAGTGGTTTACTATCATCTT+GGG | + | chr8.3:53285763-53285782 | None:intergenic | 30.0% | |
| TGAGTTATCTTTAGATGAAC+CGG | - | chr8.3:53286067-53286086 | MS.gene59888:CDS | 30.0% | |
| TTATTGAAAATTGCTGGTGA+AGG | - | chr8.3:53285897-53285916 | MS.gene59888:CDS | 30.0% | |
| !! | ATGTTCTTTGATGATGGAAA+CGG | - | chr8.3:53286104-53286123 | MS.gene59888:CDS | 30.0% |
| !! | TAGAAGAAAGTGTTACTAGT+TGG | - | chr8.3:53285721-53285740 | MS.gene59888:CDS | 30.0% |
| AGTGGTTTACTATCATCTTG+GGG | + | chr8.3:53285762-53285781 | None:intergenic | 35.0% | |
| GCTACAATGTTCTTTGATGA+TGG | - | chr8.3:53286098-53286117 | MS.gene59888:CDS | 35.0% | |
| GTAACACTTTCTTCTATTGG+AGG | + | chr8.3:53285717-53285736 | None:intergenic | 35.0% | |
| ! | ACTTTTGTCAAAAAGAGGTG+AGG | - | chr8.3:53285847-53285866 | MS.gene59888:CDS | 35.0% |
| ! | GTGCAACTTTTGTCAAAAAG+AGG | - | chr8.3:53285842-53285861 | MS.gene59888:CDS | 35.0% |
| !! | CATCATGTTCTGAGAGAAAT+TGG | - | chr8.3:53285937-53285956 | MS.gene59888:CDS | 35.0% |
| ACAGTGACAAATCTCCATAG+TGG | + | chr8.3:53285780-53285799 | None:intergenic | 40.0% | |
| ATCTTTAGATGAACCGGAGT+TGG | - | chr8.3:53286073-53286092 | MS.gene59888:CDS | 40.0% | |
| CAGAATAATGATTCTGCAGG+TGG | - | chr8.3:53285804-53285823 | MS.gene59888:CDS | 40.0% | |
| GTGCAGAATAATGATTCTGC+AGG | - | chr8.3:53285801-53285820 | MS.gene59888:CDS | 40.0% | |
| GTGCATACTAATGTTGGAGT+TGG | - | chr8.3:53286032-53286051 | MS.gene59888:CDS | 40.0% | |
| TCACTACTTCAGAGGAAACA+CGG | + | chr8.3:53286151-53286170 | None:intergenic | 40.0% | |
| TCCAACATTCACTACTTCAG+AGG | + | chr8.3:53286159-53286178 | None:intergenic | 40.0% | |
| TCGAAAAAGGGCTGAAGATT+TGG | - | chr8.3:53286004-53286023 | MS.gene59888:CDS | 40.0% | |
| ! | AGAGGAAACACGGATTGTAT+CGG | + | chr8.3:53286141-53286160 | None:intergenic | 40.0% |
| !! | TCCTCTGAAGTAGTGAATGT+TGG | - | chr8.3:53286155-53286174 | MS.gene59888:CDS | 40.0% |
| !! | TTCTTTGATGATGGAAACGG+AGG | - | chr8.3:53286107-53286126 | MS.gene59888:CDS | 40.0% |
| AACATTGTAGCCTCCAACTC+CGG | + | chr8.3:53286089-53286108 | None:intergenic | 45.0% | |
| AATTGCTGGTGAAGGAGTTC+AGG | - | chr8.3:53285905-53285924 | MS.gene59888:CDS | 45.0% | |
| TTTAGATGAACCGGAGTTGG+AGG | - | chr8.3:53286076-53286095 | MS.gene59888:CDS | 45.0% | |
| CTGCAGGTGGAAACAAGCTT+TGG | - | chr8.3:53285817-53285836 | MS.gene59888:CDS | 50.0% | |
| !! | TGATGATGGAAACGGAGGAG+AGG | - | chr8.3:53286112-53286131 | MS.gene59888:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 53285711 | 53286184 | 53285711 | ID=MS.gene59888 |
| chr8.3 | mRNA | 53285711 | 53286184 | 53285711 | ID=MS.gene59888.t1;Parent=MS.gene59888 |
| chr8.3 | exon | 53285711 | 53286184 | 53285711 | ID=MS.gene59888.t1.exon1;Parent=MS.gene59888.t1 |
| chr8.3 | CDS | 53285711 | 53286184 | 53285711 | ID=cds.MS.gene59888.t1;Parent=MS.gene59888.t1 |
| Gene Sequence |
| Protein sequence |
