Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59924.t1 | PNY05988.1 | 78.4 | 37 | 8 | 0 | 28 | 64 | 643 | 679 | 2.10E-06 | 62.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59924.t1 | A0A2K3NSH5 | 78.4 | 37 | 8 | 0 | 28 | 64 | 643 | 679 | 1.5e-06 | 62.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene59924 | 0.802848 | 4.62E-49 | -1.69E-46 |
| MS.gene050972 | MS.gene59924 | 0.929571 | 5.74E-93 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59924.t1 | MTR_4g123670 | 78.378 | 37 | 8 | 0 | 28 | 64 | 476 | 512 | 2.90e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGATTTCATATAGATTTA+AGG | 0.126167 | 8.2:+71840358 | MS.gene59924:CDS |
| CACATGACTTCCCTTGTTTC+AGG | 0.155190 | 8.2:-71839688 | None:intergenic |
| CTATATGAAATCAAAGTTAT+AGG | 0.321947 | 8.2:-71840350 | None:intergenic |
| AAACTGCTTCACTTCACTAA+TGG | 0.340507 | 8.2:+71839724 | MS.gene59924:CDS |
| GAAACGGAAGAAGAATTTCT+TGG | 0.371083 | 8.2:+71839993 | MS.gene59924:CDS |
| TTTGACAGGTTTGAGTGTAT+TGG | 0.406027 | 8.2:+71840151 | MS.gene59924:intron |
| TTATACTTGAGAGTGAGGTT+TGG | 0.466348 | 8.2:-71840208 | None:intergenic |
| TAAAGATTAACCTCAATCTT+CGG | 0.484357 | 8.2:-71839800 | None:intergenic |
| GGATGAAGACGGCGAGGATG+AGG | 0.496415 | 8.2:+71839759 | MS.gene59924:CDS |
| AAATATTATACTTGAGAGTG+AGG | 0.501284 | 8.2:-71840213 | None:intergenic |
| CAGTGAATGTTCCGCCGACA+TGG | 0.524982 | 8.2:+71839530 | MS.gene59924:CDS |
| GGTTGAAGAATATGAAGAAA+CGG | 0.528087 | 8.2:+71839977 | MS.gene59924:CDS |
| CACTAATGGAGATGAAGTAT+AGG | 0.529446 | 8.2:+71839738 | MS.gene59924:CDS |
| TCGACGGACAGCAGAGAAGA+AGG | 0.536093 | 8.2:+71839494 | MS.gene59924:CDS |
| ATTATGTTTGCAGTGGCTCA+CGG | 0.548221 | 8.2:+71840945 | MS.gene59924:intron |
| GATGATGACTCCGAAGATTG+AGG | 0.575155 | 8.2:+71839790 | MS.gene59924:CDS |
| ATTGAGCAACTCCTGAAACA+AGG | 0.592296 | 8.2:+71839677 | MS.gene59924:CDS |
| TTGAGCAACTCCTGAAACAA+GGG | 0.592522 | 8.2:+71839678 | MS.gene59924:CDS |
| TTGTTTAAATTAGGACTCTG+AGG | 0.602642 | 8.2:+71839956 | MS.gene59924:intron |
| AGTTATAGGATATGAATGCA+CGG | 0.631860 | 8.2:-71840336 | None:intergenic |
| AAACAGTTTATGATGGTCGA+CGG | 0.640458 | 8.2:+71839478 | None:intergenic |
| GATGAAGTATAGGATGAAGA+CGG | 0.641794 | 8.2:+71839748 | MS.gene59924:CDS |
| GTGAACAATATAACTCGCGA+TGG | 0.655757 | 8.2:+71840967 | MS.gene59924:CDS |
| CTGAAACAAGGGAAGTCATG+TGG | 0.662293 | 8.2:+71839689 | MS.gene59924:CDS |
| AGTATAGGATGAAGACGGCG+AGG | 0.703240 | 8.2:+71839753 | MS.gene59924:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTCAAAACATAAAAAA+TGG | - | chr8.2:71840421-71840440 | None:intergenic | 10.0% |
| !! | AAATTCAAAACATAAAAAAT+GGG | - | chr8.2:71840420-71840439 | None:intergenic | 10.0% |
| !! | TCAAATCTTAAAATTTATAT+TGG | + | chr8.2:71840082-71840101 | MS.gene59924:intron | 10.0% |
| !!! | AAAAAAATTTAAGTTTGAAA+TGG | + | chr8.2:71840810-71840829 | MS.gene59924:intron | 10.0% |
| !! | AATTCAAAACATAAAAAATG+GGG | - | chr8.2:71840419-71840438 | None:intergenic | 15.0% |
| !! | CAATAAATAAATTAAAGTCA+TGG | - | chr8.2:71839625-71839644 | None:intergenic | 15.0% |
| !! | CTTAAAAAAAATCATACAAA+AGG | - | chr8.2:71840657-71840676 | None:intergenic | 15.0% |
| !!! | CATGACTTTAATTTATTTAT+TGG | + | chr8.2:71839623-71839642 | MS.gene59924:intron | 15.0% |
| !!! | TTTGATTTCATATAGATTTA+AGG | + | chr8.2:71840358-71840377 | MS.gene59924:CDS | 15.0% |
| !! | CTATATGAAATCAAAGTTAT+AGG | - | chr8.2:71840353-71840372 | None:intergenic | 20.0% |
| !!! | AATTTATATTGGTTGAAATG+AGG | + | chr8.2:71840093-71840112 | MS.gene59924:intron | 20.0% |
| !!! | ATTTATATTGGTTGAAATGA+GGG | + | chr8.2:71840094-71840113 | MS.gene59924:intron | 20.0% |
| !!! | TTTATTATTGTTTTCAACAG+TGG | + | chr8.2:71840439-71840458 | MS.gene59924:intron | 20.0% |
| ! | AAATATTATACTTGAGAGTG+AGG | - | chr8.2:71840216-71840235 | None:intergenic | 25.0% |
| ! | ACTGTATAACTTGAATTCAT+TGG | + | chr8.2:71840858-71840877 | MS.gene59924:intron | 25.0% |
| ! | ATTAGTATCCAACAATGATT+TGG | + | chr8.2:71840625-71840644 | MS.gene59924:intron | 25.0% |
| ! | GCACATAACTAATCTAATAT+AGG | - | chr8.2:71840692-71840711 | None:intergenic | 25.0% |
| ! | TAAAGATTAACCTCAATCTT+CGG | - | chr8.2:71839803-71839822 | None:intergenic | 25.0% |
| ! | TCTAATATAGGTTCAAAAAG+TGG | - | chr8.2:71840680-71840699 | None:intergenic | 25.0% |
| !! | GTTTGAAATGGAAAGAATAT+TGG | + | chr8.2:71840822-71840841 | MS.gene59924:intron | 25.0% |
| !! | TTTTTCTATTATGTTTGCAG+TGG | + | chr8.2:71840938-71840957 | MS.gene59924:intron | 25.0% |
| !!! | AGTGTCATTATTTTGAGATT+GGG | + | chr8.2:71840541-71840560 | MS.gene59924:intron | 25.0% |
| !!! | TATTGGTTGAATTTTAGTGA+TGG | + | chr8.2:71839640-71839659 | MS.gene59924:intron | 25.0% |
| !!! | TCATGATTTTGAAATTGACT+TGG | + | chr8.2:71840506-71840525 | MS.gene59924:intron | 25.0% |
| !!! | TTTTGAAACTATTTGAGTTG+TGG | + | chr8.2:71840036-71840055 | MS.gene59924:intron | 25.0% |
| ACAACTAGATAGGATTAACT+TGG | + | chr8.2:71840467-71840486 | MS.gene59924:intron | 30.0% | |
| AGTGGTAATAACAACTAGAT+AGG | + | chr8.2:71840457-71840476 | MS.gene59924:intron | 30.0% | |
| AGTTATAGGATATGAATGCA+CGG | - | chr8.2:71840339-71840358 | None:intergenic | 30.0% | |
| CCTAATTTAAACAAATCCAC+AGG | - | chr8.2:71839950-71839969 | None:intergenic | 30.0% | |
| CTGAAAAACCAGAAGAAAAT+GGG | - | chr8.2:71840304-71840323 | None:intergenic | 30.0% | |
| GGTTGAAGAATATGAAGAAA+CGG | + | chr8.2:71839977-71839996 | MS.gene59924:CDS | 30.0% | |
| GTGAAAAAATTGAACCATGT+CGG | - | chr8.2:71839547-71839566 | None:intergenic | 30.0% | |
| TGCACAAAATAGTGAATACA+TGG | - | chr8.2:71840246-71840265 | None:intergenic | 30.0% | |
| TTCTTGGTATGATGCTATAT+GGG | + | chr8.2:71840009-71840028 | MS.gene59924:intron | 30.0% | |
| TTGTTTAAATTAGGACTCTG+AGG | + | chr8.2:71839956-71839975 | MS.gene59924:intron | 30.0% | |
| TTTAACAGTTCTTAATCCTG+TGG | + | chr8.2:71839931-71839950 | MS.gene59924:intron | 30.0% | |
| TTTCTTGGTATGATGCTATA+TGG | + | chr8.2:71840008-71840027 | MS.gene59924:intron | 30.0% | |
| ! | AATTATGTCCCATTTTCTTC+TGG | + | chr8.2:71840293-71840312 | MS.gene59924:intron | 30.0% |
| ! | CCTGTGGATTTGTTTAAATT+AGG | + | chr8.2:71839947-71839966 | MS.gene59924:intron | 30.0% |
| ! | TATATCTCCTTTTGTCTACA+AGG | + | chr8.2:71839865-71839884 | MS.gene59924:intron | 30.0% |
| !!! | GAGTGTCATTATTTTGAGAT+TGG | + | chr8.2:71840540-71840559 | MS.gene59924:intron | 30.0% |
| !!! | GTGTCATTATTTTGAGATTG+GGG | + | chr8.2:71840542-71840561 | MS.gene59924:intron | 30.0% |
| AAAAAATTGAACCATGTCGG+CGG | - | chr8.2:71839544-71839563 | None:intergenic | 35.0% | |
| AAACTGCTTCACTTCACTAA+TGG | + | chr8.2:71839724-71839743 | MS.gene59924:CDS | 35.0% | |
| ATTGACCATCTCAATTTGCA+TGG | - | chr8.2:71840901-71840920 | None:intergenic | 35.0% | |
| CACATGGCACAATATAAGTA+AGG | - | chr8.2:71840389-71840408 | None:intergenic | 35.0% | |
| CACTAATGGAGATGAAGTAT+AGG | + | chr8.2:71839738-71839757 | MS.gene59924:CDS | 35.0% | |
| CCTGAAAAACCAGAAGAAAA+TGG | - | chr8.2:71840305-71840324 | None:intergenic | 35.0% | |
| CTCTCAACAATGCTTTCATT+CGG | + | chr8.2:71840711-71840730 | MS.gene59924:intron | 35.0% | |
| GAAACGGAAGAAGAATTTCT+TGG | + | chr8.2:71839993-71840012 | MS.gene59924:CDS | 35.0% | |
| GATGAAGTATAGGATGAAGA+CGG | + | chr8.2:71839748-71839767 | MS.gene59924:CDS | 35.0% | |
| TGGGACATAATTCAGTCAAA+TGG | - | chr8.2:71840285-71840304 | None:intergenic | 35.0% | |
| TTATACTTGAGAGTGAGGTT+TGG | - | chr8.2:71840211-71840230 | None:intergenic | 35.0% | |
| TTGACCATCTCAATTTGCAT+GGG | - | chr8.2:71840900-71840919 | None:intergenic | 35.0% | |
| TTTGACAGGTTTGAGTGTAT+TGG | + | chr8.2:71840151-71840170 | MS.gene59924:intron | 35.0% | |
| ! | ATTTTCGTTCAAACCACCAT+TGG | - | chr8.2:71840607-71840626 | None:intergenic | 35.0% |
| !! | TCATTGGATGTGATTTTGAC+AGG | + | chr8.2:71840137-71840156 | MS.gene59924:intron | 35.0% |
| !! | TTGAATTTTAGTGATGGCAC+TGG | + | chr8.2:71839646-71839665 | MS.gene59924:intron | 35.0% |
| !!! | CCATTTTCTTCTGGTTTTTC+AGG | + | chr8.2:71840302-71840321 | MS.gene59924:intron | 35.0% |
| ATGAGGGTAAAGCATAGCAT+AGG | + | chr8.2:71840110-71840129 | MS.gene59924:intron | 40.0% | |
| ATTATGTTTGCAGTGGCTCA+CGG | + | chr8.2:71840945-71840964 | MS.gene59924:intron | 40.0% | |
| ATTGAGCAACTCCTGAAACA+AGG | + | chr8.2:71839677-71839696 | MS.gene59924:CDS | 40.0% | |
| CACAGAACCTTGTAGACAAA+AGG | - | chr8.2:71839875-71839894 | None:intergenic | 40.0% | |
| GGAGAGTTCCAAATCATTGT+TGG | - | chr8.2:71840636-71840655 | None:intergenic | 40.0% | |
| GTGAACAATATAACTCGCGA+TGG | + | chr8.2:71840967-71840986 | MS.gene59924:CDS | 40.0% | |
| TAGACCCATGCAAATTGAGA+TGG | + | chr8.2:71840893-71840912 | MS.gene59924:intron | 40.0% | |
| TTGAGCAACTCCTGAAACAA+GGG | + | chr8.2:71839678-71839697 | MS.gene59924:CDS | 40.0% | |
| AAAATGGGGCGCAAATCACA+TGG | - | chr8.2:71840405-71840424 | None:intergenic | 45.0% | |
| CTGAAACAAGGGAAGTCATG+TGG | + | chr8.2:71839689-71839708 | MS.gene59924:CDS | 45.0% | |
| GATGATGACTCCGAAGATTG+AGG | + | chr8.2:71839790-71839809 | MS.gene59924:CDS | 45.0% | |
| GCATAGCATAGGAGCTTCAT+TGG | + | chr8.2:71840121-71840140 | MS.gene59924:intron | 45.0% | |
| GTAGCCATATTGCCAACCAA+TGG | + | chr8.2:71840588-71840607 | MS.gene59924:intron | 45.0% | |
| TCGTTCAAACCACCATTGGT+TGG | - | chr8.2:71840603-71840622 | None:intergenic | 45.0% | |
| ! | ACCACCATTGGTTGGCAATA+TGG | - | chr8.2:71840595-71840614 | None:intergenic | 45.0% |
| ! | CACATGACTTCCCTTGTTTC+AGG | - | chr8.2:71839691-71839710 | None:intergenic | 45.0% |
| !!! | ATTTTGAGATTGGGGCTGCT+TGG | + | chr8.2:71840550-71840569 | MS.gene59924:intron | 45.0% |
| AGTATAGGATGAAGACGGCG+AGG | + | chr8.2:71839753-71839772 | MS.gene59924:CDS | 50.0% | |
| GCCATATTGCCAACCAATGG+TGG | + | chr8.2:71840591-71840610 | MS.gene59924:intron | 50.0% | |
| CAGTGAATGTTCCGCCGACA+TGG | + | chr8.2:71839530-71839549 | MS.gene59924:CDS | 55.0% | |
| TCGACGGACAGCAGAGAAGA+AGG | + | chr8.2:71839494-71839513 | MS.gene59924:CDS | 55.0% | |
| GGATGAAGACGGCGAGGATG+AGG | + | chr8.2:71839759-71839778 | MS.gene59924:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 71839487 | 71841002 | 71839487 | ID=MS.gene59924 |
| chr8.2 | mRNA | 71839487 | 71841002 | 71839487 | ID=MS.gene59924.t1;Parent=MS.gene59924 |
| chr8.2 | exon | 71839487 | 71839568 | 71839487 | ID=MS.gene59924.t1.exon1;Parent=MS.gene59924.t1 |
| chr8.2 | CDS | 71839487 | 71839568 | 71839487 | ID=cds.MS.gene59924.t1;Parent=MS.gene59924.t1 |
| chr8.2 | exon | 71839657 | 71839811 | 71839657 | ID=MS.gene59924.t1.exon2;Parent=MS.gene59924.t1 |
| chr8.2 | CDS | 71839657 | 71839811 | 71839657 | ID=cds.MS.gene59924.t1;Parent=MS.gene59924.t1 |
| chr8.2 | exon | 71839969 | 71840014 | 71839969 | ID=MS.gene59924.t1.exon3;Parent=MS.gene59924.t1 |
| chr8.2 | CDS | 71839969 | 71840014 | 71839969 | ID=cds.MS.gene59924.t1;Parent=MS.gene59924.t1 |
| chr8.2 | exon | 71840159 | 71840224 | 71840159 | ID=MS.gene59924.t1.exon4;Parent=MS.gene59924.t1 |
| chr8.2 | CDS | 71840159 | 71840224 | 71840159 | ID=cds.MS.gene59924.t1;Parent=MS.gene59924.t1 |
| chr8.2 | exon | 71840324 | 71840379 | 71840324 | ID=MS.gene59924.t1.exon5;Parent=MS.gene59924.t1 |
| chr8.2 | CDS | 71840324 | 71840379 | 71840324 | ID=cds.MS.gene59924.t1;Parent=MS.gene59924.t1 |
| chr8.2 | exon | 71840958 | 71841002 | 71840958 | ID=MS.gene59924.t1.exon6;Parent=MS.gene59924.t1 |
| chr8.2 | CDS | 71840958 | 71841002 | 71840958 | ID=cds.MS.gene59924.t1;Parent=MS.gene59924.t1 |
| Gene Sequence |
| Protein sequence |
