Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61223.t1 | XP_003597762.1 | 90.9 | 121 | 4 | 3 | 1 | 119 | 88 | 203 | 5.20E-45 | 190.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61223.t1 | G7II32 | 90.9 | 121 | 4 | 3 | 1 | 119 | 88 | 203 | 3.8e-45 | 190.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene61223 | 0.8392 | 1.83E-57 | -1.69E-46 |
| MS.gene055466 | MS.gene61223 | 0.802875 | 4.56E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61223.t1 | MTR_2g101980 | 83.471 | 121 | 13 | 3 | 1 | 119 | 88 | 203 | 2.28e-59 | 181 |
| MS.gene61223.t1 | MTR_0147s0050 | 52.419 | 124 | 51 | 4 | 1 | 119 | 82 | 202 | 1.14e-32 | 114 |
| MS.gene61223.t1 | MTR_0147s0020 | 68.182 | 66 | 21 | 0 | 39 | 104 | 2 | 67 | 1.59e-27 | 99.0 |
| MS.gene61223.t1 | MTR_8g103530 | 50.000 | 72 | 32 | 2 | 48 | 118 | 134 | 202 | 3.91e-16 | 71.2 |
| MS.gene61223.t1 | MTR_6g086915 | 43.011 | 93 | 44 | 2 | 9 | 95 | 79 | 168 | 5.71e-14 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61223.t1 | AT1G15260 | 44.737 | 114 | 48 | 5 | 6 | 119 | 80 | 178 | 9.56e-20 | 80.1 |
| MS.gene61223.t1 | AT3G16070 | 63.830 | 47 | 17 | 0 | 48 | 94 | 104 | 150 | 4.56e-18 | 75.1 |
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCACAATATCTAAATAAAT+AGG | 0.347318 | 2.3:+2124809 | None:intergenic |
| GAATTAGAGATGAATGATTC+AGG | 0.387057 | 2.3:-2124893 | MS.gene61223:CDS |
| GAATGAAGGTGTTAATGAAT+TGG | 0.390905 | 2.3:-2124930 | MS.gene61223:CDS |
| TCTAAGTCAAAGGGAAGATT+TGG | 0.402476 | 2.3:-2124722 | MS.gene61223:CDS |
| AGGGAAGATTTGGATCAATC+AGG | 0.432753 | 2.3:-2124712 | MS.gene61223:CDS |
| TCCTATTTATTTAGATATTG+TGG | 0.439985 | 2.3:-2124810 | MS.gene61223:CDS |
| TGAATTGGCATTGAAGATGA+AGG | 0.456133 | 2.3:-2124915 | MS.gene61223:CDS |
| AAAGGGAATAAGAAGAATGA+AGG | 0.496505 | 2.3:-2124944 | MS.gene61223:CDS |
| ATCAAACGCTCTAAGTCAAA+GGG | 0.509484 | 2.3:-2124731 | MS.gene61223:CDS |
| CATCAAACGCTCTAAGTCAA+AGG | 0.517132 | 2.3:-2124732 | MS.gene61223:CDS |
| AGAGCGTTTGATGCTGACAG+AGG | 0.645792 | 2.3:+2124742 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCCACAATATCTAAATAAAT+AGG | + | chr2.3:2124943-2124962 | None:intergenic | 20.0% |
| !! | TCCTATTTATTTAGATATTG+TGG | - | chr2.3:2124939-2124958 | MS.gene61223:CDS | 20.0% |
| ! | AGAAAGAGAAAAAAGACAAA+GGG | - | chr2.3:2124788-2124807 | MS.gene61223:CDS | 25.0% |
| ! | CAAAAAAACCTTACTGAAAA+TGG | - | chr2.3:2124763-2124782 | MS.gene61223:CDS | 25.0% |
| !!! | TTTTCAGTAAGGTTTTTTTG+TGG | + | chr2.3:2124763-2124782 | None:intergenic | 25.0% |
| AAAGGGAATAAGAAGAATGA+AGG | - | chr2.3:2124805-2124824 | MS.gene61223:CDS | 30.0% | |
| GAATTAGAGATGAATGATTC+AGG | - | chr2.3:2124856-2124875 | MS.gene61223:CDS | 30.0% | |
| GAGAAAGAGAAAAAAGACAA+AGG | - | chr2.3:2124787-2124806 | MS.gene61223:CDS | 30.0% | |
| !! | GAATGAAGGTGTTAATGAAT+TGG | - | chr2.3:2124819-2124838 | MS.gene61223:CDS | 30.0% |
| TCTAAGTCAAAGGGAAGATT+TGG | - | chr2.3:2125027-2125046 | MS.gene61223:CDS | 35.0% | |
| ! | ATCAAACGCTCTAAGTCAAA+GGG | - | chr2.3:2125018-2125037 | MS.gene61223:CDS | 35.0% |
| ! | CTTTCTCTCCATTTTCAGTA+AGG | + | chr2.3:2124774-2124793 | None:intergenic | 35.0% |
| ! | TGAATTGGCATTGAAGATGA+AGG | - | chr2.3:2124834-2124853 | MS.gene61223:CDS | 35.0% |
| AGGGAAGATTTGGATCAATC+AGG | - | chr2.3:2125037-2125056 | MS.gene61223:CDS | 40.0% | |
| ! | CATCAAACGCTCTAAGTCAA+AGG | - | chr2.3:2125017-2125036 | MS.gene61223:CDS | 40.0% |
| ! | AGAGCGTTTGATGCTGACAG+AGG | + | chr2.3:2125010-2125029 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 2124706 | 2125065 | 2124706 | ID=MS.gene61223 |
| chr2.3 | mRNA | 2124706 | 2125065 | 2124706 | ID=MS.gene61223.t1;Parent=MS.gene61223 |
| chr2.3 | exon | 2124706 | 2125065 | 2124706 | ID=MS.gene61223.t1.exon1;Parent=MS.gene61223.t1 |
| chr2.3 | CDS | 2124706 | 2125065 | 2124706 | ID=cds.MS.gene61223.t1;Parent=MS.gene61223.t1 |
| Gene Sequence |
| Protein sequence |
