Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61467.t1 | XP_003609259.1 | 95.3 | 107 | 5 | 0 | 1 | 107 | 1 | 107 | 4.50E-56 | 226.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61467.t1 | G7JVA0 | 95.3 | 107 | 5 | 0 | 1 | 107 | 1 | 107 | 3.3e-56 | 226.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene61467 | 0.801834 | 7.47E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene61467 | 0.809823 | 1.56E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61467.t1 | MTR_4g113750 | 95.327 | 107 | 5 | 0 | 1 | 107 | 1 | 107 | 1.81e-73 | 213 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61467.t1 | AT1G75125 | 59.574 | 94 | 38 | 0 | 13 | 106 | 11 | 104 | 1.69e-39 | 127 |
Find 28 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGGAAATCGCCTTGGAAT+TGG | 0.320897 | 8.3:+13503910 | None:intergenic |
| GTCCTACTTGCGATGGAAAC+AGG | 0.354255 | 8.3:-13503568 | MS.gene61467:intron |
| ACTTTGTGTCTGAGAATAAA+CGG | 0.389072 | 8.3:-13503807 | MS.gene61467:CDS |
| CGATTTCCAACATGGATTAC+CGG | 0.398072 | 8.3:-13503898 | MS.gene61467:CDS |
| ACAACGTTGTCTGAATTGTC+TGG | 0.418889 | 8.3:-13503441 | MS.gene61467:CDS |
| ACACATGGGTTAATTGCAAT+AGG | 0.462234 | 8.3:+13503593 | None:intergenic |
| CTTTGTGTCTGAGAATAAAC+GGG | 0.471497 | 8.3:-13503806 | MS.gene61467:intron |
| TGTCTGAATTGTCTGGGAAA+AGG | 0.496121 | 8.3:-13503434 | MS.gene61467:CDS |
| TTCCAAGGCGATTTCCAACA+TGG | 0.513408 | 8.3:-13503906 | MS.gene61467:CDS |
| ATCCATGTTGGAAATCGCCT+TGG | 0.531843 | 8.3:+13503904 | None:intergenic |
| TGTAAAAGTTGTAGAGGGAA+AGG | 0.556567 | 8.3:-13503677 | MS.gene61467:CDS |
| ATATATGCAAGTTATGCAGT+GGG | 0.557283 | 8.3:-13503649 | MS.gene61467:CDS |
| GTAAAAGTTGTAGAGGGAAA+GGG | 0.562827 | 8.3:-13503676 | MS.gene61467:CDS |
| TATATATGCAAGTTATGCAG+TGG | 0.562975 | 8.3:-13503650 | MS.gene61467:CDS |
| CAACGTTGTCTGAATTGTCT+GGG | 0.565752 | 8.3:-13503440 | MS.gene61467:CDS |
| CCCCAATATTACAAAAGCAC+CGG | 0.566726 | 8.3:+13503879 | None:intergenic |
| GTGGGAATTCAACCATAGCT+TGG | 0.572794 | 8.3:-13503631 | MS.gene61467:CDS |
| AAAGCACCGGTAATCCATGT+TGG | 0.590454 | 8.3:+13503892 | None:intergenic |
| TGTGTTTGTCCTACTTGCGA+TGG | 0.598415 | 8.3:-13503575 | MS.gene61467:CDS |
| TTGCCGTGTAAAAGTTGTAG+AGG | 0.598560 | 8.3:-13503683 | MS.gene61467:CDS |
| AAGAAATGAATCCAATTCCA+AGG | 0.602574 | 8.3:-13503921 | None:intergenic |
| ATTGCAATAGGATCATACAT+TGG | 0.616790 | 8.3:+13503605 | None:intergenic |
| ATACATTGGAGACCAAGCTA+TGG | 0.629079 | 8.3:+13503619 | None:intergenic |
| TCGCAAGTAGGACAAACACA+TGG | 0.629902 | 8.3:+13503578 | None:intergenic |
| GGGAATTGCAACTCTGAACG+TGG | 0.644672 | 8.3:-13503857 | MS.gene61467:CDS |
| TGCCGTGTAAAAGTTGTAGA+GGG | 0.654823 | 8.3:-13503682 | MS.gene61467:CDS |
| CGCAAGTAGGACAAACACAT+GGG | 0.656170 | 8.3:+13503579 | None:intergenic |
| AACCTGTTTCCATCGCAAGT+AGG | 0.668457 | 8.3:+13503566 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAATTGCAATTGATAA+TGG | - | chr8.3:13503862-13503881 | MS.gene61467:CDS | 20.0% |
| !! | TTTGCTTTCATTTGAATATT+GGG | - | chr8.3:13503824-13503843 | MS.gene61467:CDS | 20.0% |
| !!! | TTTTGCTTTCATTTGAATAT+TGG | - | chr8.3:13503823-13503842 | MS.gene61467:CDS | 20.0% |
| ! | TTGCTTTCATTTGAATATTG+GGG | - | chr8.3:13503825-13503844 | MS.gene61467:CDS | 25.0% |
| ACTTTGTGTCTGAGAATAAA+CGG | - | chr8.3:13503533-13503552 | MS.gene61467:intron | 30.0% | |
| ATATATGCAAGTTATGCAGT+GGG | - | chr8.3:13503691-13503710 | MS.gene61467:CDS | 30.0% | |
| ATTGCAATAGGATCATACAT+TGG | + | chr8.3:13503738-13503757 | None:intergenic | 30.0% | |
| GATAATATTGATGTGCTTTC+TGG | - | chr8.3:13503601-13503620 | MS.gene61467:CDS | 30.0% | |
| TATATATGCAAGTTATGCAG+TGG | - | chr8.3:13503690-13503709 | MS.gene61467:CDS | 30.0% | |
| !!! | ATTGATAATGGTTTTGTTGC+AGG | - | chr8.3:13503874-13503893 | MS.gene61467:CDS | 30.0% |
| !!! | TTGATAATGGTTTTGTTGCA+GGG | - | chr8.3:13503875-13503894 | MS.gene61467:CDS | 30.0% |
| CTTTGTGTCTGAGAATAAAC+GGG | - | chr8.3:13503534-13503553 | MS.gene61467:intron | 35.0% | |
| GTAAAAGTTGTAGAGGGAAA+GGG | - | chr8.3:13503664-13503683 | MS.gene61467:CDS | 35.0% | |
| TGTAAAAGTTGTAGAGGGAA+AGG | - | chr8.3:13503663-13503682 | MS.gene61467:CDS | 35.0% | |
| ! | ACACATGGGTTAATTGCAAT+AGG | + | chr8.3:13503750-13503769 | None:intergenic | 35.0% |
| ! | ACCGGTGCTTTTGTAATATT+GGG | - | chr8.3:13503460-13503479 | MS.gene61467:intron | 35.0% |
| ! | TACCGGTGCTTTTGTAATAT+TGG | - | chr8.3:13503459-13503478 | MS.gene61467:intron | 35.0% |
| ! | TTCCCTCTACAACTTTTACA+CGG | + | chr8.3:13503663-13503682 | None:intergenic | 35.0% |
| ! | TTGATGTGCTTTCTGGAAAT+TGG | - | chr8.3:13503608-13503627 | MS.gene61467:CDS | 35.0% |
| ACAACGTTGTCTGAATTGTC+TGG | - | chr8.3:13503899-13503918 | MS.gene61467:CDS | 40.0% | |
| ATACATTGGAGACCAAGCTA+TGG | + | chr8.3:13503724-13503743 | None:intergenic | 40.0% | |
| CAACGTTGTCTGAATTGTCT+GGG | - | chr8.3:13503900-13503919 | MS.gene61467:CDS | 40.0% | |
| TGCCGTGTAAAAGTTGTAGA+GGG | - | chr8.3:13503658-13503677 | MS.gene61467:CDS | 40.0% | |
| TGTCTGAATTGTCTGGGAAA+AGG | - | chr8.3:13503906-13503925 | MS.gene61467:CDS | 40.0% | |
| TTGCCGTGTAAAAGTTGTAG+AGG | - | chr8.3:13503657-13503676 | MS.gene61467:CDS | 40.0% | |
| ! | CCCCAATATTACAAAAGCAC+CGG | + | chr8.3:13503464-13503483 | None:intergenic | 40.0% |
| ! | CCGGTGCTTTTGTAATATTG+GGG | - | chr8.3:13503461-13503480 | MS.gene61467:intron | 40.0% |
| ! | CGATTTCCAACATGGATTAC+CGG | - | chr8.3:13503442-13503461 | MS.gene61467:CDS | 40.0% |
| ! | CGGTGCTTTTGTAATATTGG+GGG | - | chr8.3:13503462-13503481 | MS.gene61467:intron | 40.0% |
| ! | GGTGCTTTTGTAATATTGGG+GGG | - | chr8.3:13503463-13503482 | MS.gene61467:intron | 40.0% |
| AACCTGTTTCCATCGCAAGT+AGG | + | chr8.3:13503777-13503796 | None:intergenic | 45.0% | |
| ATCCATGTTGGAAATCGCCT+TGG | + | chr8.3:13503439-13503458 | None:intergenic | 45.0% | |
| CGCAAGTAGGACAAACACAT+GGG | + | chr8.3:13503764-13503783 | None:intergenic | 45.0% | |
| GTGGGAATTCAACCATAGCT+TGG | - | chr8.3:13503709-13503728 | MS.gene61467:intron | 45.0% | |
| GTTGGAAATCGCCTTGGAAT+TGG | + | chr8.3:13503433-13503452 | None:intergenic | 45.0% | |
| TCGCAAGTAGGACAAACACA+TGG | + | chr8.3:13503765-13503784 | None:intergenic | 45.0% | |
| TGTGTTTGTCCTACTTGCGA+TGG | - | chr8.3:13503765-13503784 | MS.gene61467:intron | 45.0% | |
| TTCCAAGGCGATTTCCAACA+TGG | - | chr8.3:13503434-13503453 | MS.gene61467:CDS | 45.0% | |
| !! | AAAGCACCGGTAATCCATGT+TGG | + | chr8.3:13503451-13503470 | None:intergenic | 45.0% |
| GGGAATTGCAACTCTGAACG+TGG | - | chr8.3:13503483-13503502 | MS.gene61467:intron | 50.0% | |
| GTCCTACTTGCGATGGAAAC+AGG | - | chr8.3:13503772-13503791 | MS.gene61467:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 13503424 | 13503938 | 13503424 | ID=MS.gene61467 |
| chr8.3 | mRNA | 13503424 | 13503938 | 13503424 | ID=MS.gene61467.t1;Parent=MS.gene61467 |
| chr8.3 | exon | 13503807 | 13503938 | 13503807 | ID=MS.gene61467.t1.exon1;Parent=MS.gene61467.t1 |
| chr8.3 | CDS | 13503807 | 13503938 | 13503807 | ID=cds.MS.gene61467.t1;Parent=MS.gene61467.t1 |
| chr8.3 | exon | 13503569 | 13503717 | 13503569 | ID=MS.gene61467.t1.exon2;Parent=MS.gene61467.t1 |
| chr8.3 | CDS | 13503569 | 13503717 | 13503569 | ID=cds.MS.gene61467.t1;Parent=MS.gene61467.t1 |
| chr8.3 | exon | 13503424 | 13503466 | 13503424 | ID=MS.gene61467.t1.exon3;Parent=MS.gene61467.t1 |
| chr8.3 | CDS | 13503424 | 13503466 | 13503424 | ID=cds.MS.gene61467.t1;Parent=MS.gene61467.t1 |
| Gene Sequence |
| Protein sequence |
