Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62027.t1 | XP_024642190.1 | 91.2 | 136 | 12 | 0 | 1 | 136 | 773 | 908 | 6.60E-68 | 266.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62027.t1 | A0A2Z6MHT1 | 69.9 | 136 | 41 | 0 | 1 | 136 | 712 | 847 | 7.9e-47 | 196.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene62027 | 0.80049 | 1.41E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCATTTCTAACACTTAT+TGG | 0.204450 | 7.2:+79952453 | None:intergenic |
| CTCGGTCGTCCGTCTGGGTC+TGG | 0.226717 | 7.2:-79952381 | MS.gene62027:CDS |
| CGTCTTCTCGGTCGTCCGTC+TGG | 0.291935 | 7.2:-79952387 | MS.gene62027:CDS |
| CAAATGTAATATATGCATAT+TGG | 0.294250 | 7.2:+79952191 | None:intergenic |
| TTATCAGTCTTTCGTCTTCT+CGG | 0.336748 | 7.2:-79952399 | MS.gene62027:CDS |
| ACTTGATCTTTAAACCATCT+TGG | 0.349729 | 7.2:+79952228 | None:intergenic |
| AAATGTAATATATGCATATT+GGG | 0.354988 | 7.2:+79952192 | None:intergenic |
| TCTTTCAAGTATTAAATGTT+GGG | 0.365809 | 7.2:-79952269 | MS.gene62027:CDS |
| TGACGGACTCCATTCACTTT+CGG | 0.384253 | 7.2:+79952425 | None:intergenic |
| TCGGTCGTCCGTCTGGGTCT+GGG | 0.388874 | 7.2:-79952380 | MS.gene62027:CDS |
| GTCTTCTCGGTCGTCCGTCT+GGG | 0.395396 | 7.2:-79952386 | MS.gene62027:CDS |
| ATCTTTCAAGTATTAAATGT+TGG | 0.415727 | 7.2:-79952270 | MS.gene62027:CDS |
| CTGGGTCTGGGAAAGAGTAC+TGG | 0.466946 | 7.2:-79952368 | MS.gene62027:CDS |
| TAATTGCAGCTTACTGTGCT+CGG | 0.476241 | 7.2:-79952524 | MS.gene62027:CDS |
| AAGCCATTGCCGAAAGTGAA+TGG | 0.483149 | 7.2:-79952434 | MS.gene62027:CDS |
| ACTCCATTCACTTTCGGCAA+TGG | 0.490970 | 7.2:+79952431 | None:intergenic |
| AACAACAAAACAAGGGTTGA+AGG | 0.508561 | 7.2:-79952552 | MS.gene62027:CDS |
| TTGATTAACTGTCATGAAGT+TGG | 0.519513 | 7.2:-79952306 | MS.gene62027:CDS |
| CGAAAGACTGATAAGGTTGA+CGG | 0.526680 | 7.2:+79952408 | None:intergenic |
| AATTGCAGCTTACTGTGCTC+GGG | 0.530087 | 7.2:-79952523 | MS.gene62027:CDS |
| ACTTGAAAGATGTGAAGGTA+TGG | 0.538062 | 7.2:+79952282 | None:intergenic |
| CATTTGAGAGGATTAGCTGA+TGG | 0.554077 | 7.2:-79952174 | None:intergenic |
| CTTTCAAGTATTAAATGTTG+GGG | 0.556290 | 7.2:-79952268 | MS.gene62027:CDS |
| GAACGGTGAACAACAAAACA+AGG | 0.559715 | 7.2:-79952560 | MS.gene62027:CDS |
| GGATGAACGATGCAGAGTTA+CGG | 0.562206 | 7.2:-79952347 | MS.gene62027:CDS |
| ATGCATATATTACATTTGAG+AGG | 0.565395 | 7.2:-79952186 | MS.gene62027:CDS |
| TTAATACTTGAAAGATGTGA+AGG | 0.567579 | 7.2:+79952277 | None:intergenic |
| GAGAAGACGAAAGACTGATA+AGG | 0.587671 | 7.2:+79952401 | None:intergenic |
| GTTAATCAACACATGAACTT+CGG | 0.607117 | 7.2:+79952320 | None:intergenic |
| TACTCTTTCCCAGACCCAGA+CGG | 0.640150 | 7.2:+79952372 | None:intergenic |
| GAACGATGCAGAGTTACGGT+CGG | 0.676176 | 7.2:-79952343 | MS.gene62027:CDS |
| AACGGTGAACAACAAAACAA+GGG | 0.692708 | 7.2:-79952559 | MS.gene62027:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGTAATATATGCATATT+GGG | + | chr7.2:79952547-79952566 | None:intergenic | 15.0% |
| !! | CAAATGTAATATATGCATAT+TGG | + | chr7.2:79952548-79952567 | None:intergenic | 20.0% |
| !!! | ATCTTTCAAGTATTAAATGT+TGG | - | chr7.2:79952466-79952485 | MS.gene62027:CDS | 20.0% |
| !!! | TCTTTCAAGTATTAAATGTT+GGG | - | chr7.2:79952467-79952486 | MS.gene62027:CDS | 20.0% |
| ! | ATGCATATATTACATTTGAG+AGG | - | chr7.2:79952550-79952569 | MS.gene62027:CDS | 25.0% |
| ! | GTAATGAGAACAAAAATTCA+AGG | + | chr7.2:79952242-79952261 | None:intergenic | 25.0% |
| ! | TTAATACTTGAAAGATGTGA+AGG | + | chr7.2:79952462-79952481 | None:intergenic | 25.0% |
| !! | CTTTCAAGTATTAAATGTTG+GGG | - | chr7.2:79952468-79952487 | MS.gene62027:CDS | 25.0% |
| ACTTGATCTTTAAACCATCT+TGG | + | chr7.2:79952511-79952530 | None:intergenic | 30.0% | |
| GCTTCATTTCTAACACTTAT+TGG | + | chr7.2:79952286-79952305 | None:intergenic | 30.0% | |
| GTTAATCAACACATGAACTT+CGG | + | chr7.2:79952419-79952438 | None:intergenic | 30.0% | |
| TTGATTAACTGTCATGAAGT+TGG | - | chr7.2:79952430-79952449 | MS.gene62027:CDS | 30.0% | |
| !!! | TATACACTAGTTTTCCAAGA+TGG | - | chr7.2:79952494-79952513 | MS.gene62027:CDS | 30.0% |
| AACAACAAAACAAGGGTTGA+AGG | - | chr7.2:79952184-79952203 | MS.gene62027:CDS | 35.0% | |
| AACGGTGAACAACAAAACAA+GGG | - | chr7.2:79952177-79952196 | MS.gene62027:CDS | 35.0% | |
| ACTTGAAAGATGTGAAGGTA+TGG | + | chr7.2:79952457-79952476 | None:intergenic | 35.0% | |
| TTATCAGTCTTTCGTCTTCT+CGG | - | chr7.2:79952337-79952356 | MS.gene62027:CDS | 35.0% | |
| CGAAAGACTGATAAGGTTGA+CGG | + | chr7.2:79952331-79952350 | None:intergenic | 40.0% | |
| GAACGGTGAACAACAAAACA+AGG | - | chr7.2:79952176-79952195 | MS.gene62027:CDS | 40.0% | |
| GAGAAGACGAAAGACTGATA+AGG | + | chr7.2:79952338-79952357 | None:intergenic | 40.0% | |
| TAATTGCAGCTTACTGTGCT+CGG | - | chr7.2:79952212-79952231 | MS.gene62027:CDS | 40.0% | |
| AAGCCATTGCCGAAAGTGAA+TGG | - | chr7.2:79952302-79952321 | MS.gene62027:CDS | 45.0% | |
| AATTGCAGCTTACTGTGCTC+GGG | - | chr7.2:79952213-79952232 | MS.gene62027:CDS | 45.0% | |
| ACTCCATTCACTTTCGGCAA+TGG | + | chr7.2:79952308-79952327 | None:intergenic | 45.0% | |
| GGATGAACGATGCAGAGTTA+CGG | - | chr7.2:79952389-79952408 | MS.gene62027:CDS | 45.0% | |
| !! | TGACGGACTCCATTCACTTT+CGG | + | chr7.2:79952314-79952333 | None:intergenic | 45.0% |
| GAACGATGCAGAGTTACGGT+CGG | - | chr7.2:79952393-79952412 | MS.gene62027:CDS | 50.0% | |
| TACTCTTTCCCAGACCCAGA+CGG | + | chr7.2:79952367-79952386 | None:intergenic | 50.0% | |
| ! | CTGGGTCTGGGAAAGAGTAC+TGG | - | chr7.2:79952368-79952387 | MS.gene62027:CDS | 55.0% |
| GTCTTCTCGGTCGTCCGTCT+GGG | - | chr7.2:79952350-79952369 | MS.gene62027:CDS | 60.0% | |
| CGTCTTCTCGGTCGTCCGTC+TGG | - | chr7.2:79952349-79952368 | MS.gene62027:CDS | 65.0% | |
| TCGGTCGTCCGTCTGGGTCT+GGG | - | chr7.2:79952356-79952375 | MS.gene62027:CDS | 65.0% | |
| CTCGGTCGTCCGTCTGGGTC+TGG | - | chr7.2:79952355-79952374 | MS.gene62027:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 79952175 | 79952583 | 79952175 | ID=MS.gene62027 |
| chr7.2 | mRNA | 79952175 | 79952583 | 79952175 | ID=MS.gene62027.t1;Parent=MS.gene62027 |
| chr7.2 | exon | 79952175 | 79952583 | 79952175 | ID=MS.gene62027.t1.exon1;Parent=MS.gene62027.t1 |
| chr7.2 | CDS | 79952175 | 79952583 | 79952175 | ID=cds.MS.gene62027.t1;Parent=MS.gene62027.t1 |
| Gene Sequence |
| Protein sequence |
