Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62140.t1 | PNX75878.1 | 73.3 | 90 | 24 | 0 | 1 | 90 | 1 | 90 | 2.60E-22 | 114.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62140.t1 | A0A2K3LBG3 | 73.3 | 90 | 24 | 0 | 1 | 90 | 1 | 90 | 1.9e-22 | 114.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050502 | MS.gene62140 | 0.806123 | 9.59E-50 | -1.69E-46 |
| MS.gene059517 | MS.gene62140 | 0.814914 | 1.21E-51 | -1.69E-46 |
| MS.gene060372 | MS.gene62140 | 0.807074 | 6.04E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATGAGTTGGTGGTAATTA+AGG | 0.235955 | 5.1:+36404938 | None:intergenic |
| CATAGCAGGGACACATTTCT+TGG | 0.264407 | 5.1:+36405022 | None:intergenic |
| TCTCCATCTTGTTCATGCTT+TGG | 0.293177 | 5.1:-36405719 | MS.gene62140:CDS |
| GGTGGTTTGGTTGGCATAGC+AGG | 0.303830 | 5.1:+36405008 | None:intergenic |
| GGCACTTGCGTCTGCCTCCA+TGG | 0.330551 | 5.1:+36404986 | None:intergenic |
| TGCGTGCCACCAGGAACTTC+TGG | 0.331803 | 5.1:-36405048 | MS.gene62140:CDS |
| CACGGCCGCGCCTCTGCCAC+AGG | 0.395156 | 5.1:-36405101 | MS.gene62140:CDS |
| TCTTGGTTACCAGAAGTTCC+TGG | 0.400394 | 5.1:+36405039 | None:intergenic |
| GTCTGCCTCCATGGGTGGTT+TGG | 0.427775 | 5.1:+36404995 | None:intergenic |
| GCCTCCATGGGTGGTTTGGT+TGG | 0.435015 | 5.1:+36404999 | None:intergenic |
| TTCCACATGCCCTGTGGCAG+AGG | 0.454808 | 5.1:+36405091 | None:intergenic |
| GGCGCGGCCGTGATGATAAA+CGG | 0.465640 | 5.1:+36405112 | None:intergenic |
| CGCCTCTGCCACAGGGCATG+TGG | 0.480960 | 5.1:-36405093 | MS.gene62140:CDS |
| GCACTTGCGTCTGCCTCCAT+GGG | 0.498020 | 5.1:+36404987 | None:intergenic |
| CTTGCGTCTGCCTCCATGGG+TGG | 0.501890 | 5.1:+36404990 | None:intergenic |
| TGATGATTCCACTCAATTCA+AGG | 0.504589 | 5.1:+36404964 | None:intergenic |
| CGGTGCAACTGCGTGCCACC+AGG | 0.530335 | 5.1:-36405057 | MS.gene62140:CDS |
| AGCATGAACAAGATGGAGAA+TGG | 0.540714 | 5.1:+36405723 | None:intergenic |
| GTGGTTTGGTTGGCATAGCA+GGG | 0.546195 | 5.1:+36405009 | None:intergenic |
| TGTTCATGCTTTGGACCAGC+CGG | 0.555470 | 5.1:-36405710 | MS.gene62140:intron |
| GCAATGGAGCATGTGCTGCT+AGG | 0.562822 | 5.1:-36405137 | MS.gene62140:CDS |
| AGTTGCATTGCATGAGTTGG+TGG | 0.565582 | 5.1:+36404929 | None:intergenic |
| ATATAAATTTCAGATTGCAA+TGG | 0.566444 | 5.1:-36405153 | MS.gene62140:intron |
| GGTCCAAAGCATGAACAAGA+TGG | 0.576917 | 5.1:+36405716 | None:intergenic |
| ATAAGTTGCATTGCATGAGT+TGG | 0.582143 | 5.1:+36404926 | None:intergenic |
| CATGCCCTGTGGCAGAGGCG+CGG | 0.598879 | 5.1:+36405096 | None:intergenic |
| GATGATTCCACTCAATTCAA+GGG | 0.603193 | 5.1:+36404965 | None:intergenic |
| GCAAGTGCCCTTGAATTGAG+TGG | 0.613235 | 5.1:-36404972 | MS.gene62140:CDS |
| CATGTGGAACTTGCTGCAGT+CGG | 0.613537 | 5.1:-36405077 | MS.gene62140:CDS |
| ACGGCCGCGCCTCTGCCACA+GGG | 0.616011 | 5.1:-36405100 | MS.gene62140:CDS |
| CTAGGTGCCGTTTATCATCA+CGG | 0.637583 | 5.1:-36405119 | MS.gene62140:CDS |
| TATGCCAACCAAACCACCCA+TGG | 0.640986 | 5.1:-36405003 | MS.gene62140:CDS |
| TGGTTACCAGAAGTTCCTGG+TGG | 0.663688 | 5.1:+36405042 | None:intergenic |
| GCCAACCAAACCACCCATGG+AGG | 0.699460 | 5.1:-36405000 | MS.gene62140:CDS |
| AGCAAGTTCCACATGCCCTG+TGG | 0.700479 | 5.1:+36405085 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATATGAACTAAACTA+AGG | - | chr5.1:36405247-36405266 | MS.gene62140:intron | 15.0% |
| !! | TGGAAAAAATATAAAAAAAC+TGG | - | chr5.1:36405201-36405220 | MS.gene62140:intron | 15.0% |
| !!! | TTTTAATTATTTGCTTAAAG+TGG | - | chr5.1:36405181-36405200 | MS.gene62140:intron | 15.0% |
| !! | ATATAAATTTCAGATTGCAA+TGG | - | chr5.1:36405521-36405540 | MS.gene62140:intron | 20.0% |
| !! | GGATAAAAGATTAGTAAAAA+AGG | - | chr5.1:36405361-36405380 | MS.gene62140:intron | 20.0% |
| !!! | TATTTTATAAAGAAAACGCT+TGG | + | chr5.1:36405039-36405058 | None:intergenic | 20.0% |
| ! | TAACGAATATGTATGCTAAT+GGG | - | chr5.1:36405340-36405359 | MS.gene62140:intron | 25.0% |
| ! | TTAACGAATATGTATGCTAA+TGG | - | chr5.1:36405339-36405358 | MS.gene62140:intron | 25.0% |
| !! | TTAATTACATGTGTTTTCGT+AGG | - | chr5.1:36405075-36405094 | MS.gene62140:CDS | 25.0% |
| !! | CAACAATAGCTTTGAATTAC+CGG | + | chr5.1:36404986-36405005 | None:intergenic | 30.0% |
| ATAAGTTGCATTGCATGAGT+TGG | + | chr5.1:36405751-36405770 | None:intergenic | 35.0% | |
| GATGATTCCACTCAATTCAA+GGG | + | chr5.1:36405712-36405731 | None:intergenic | 35.0% | |
| TGATGATTCCACTCAATTCA+AGG | + | chr5.1:36405713-36405732 | None:intergenic | 35.0% | |
| ! | CTACTTCACACACGTATTTT+TGG | + | chr5.1:36405489-36405508 | None:intergenic | 35.0% |
| AGCATGAACAAGATGGAGAA+TGG | + | chr5.1:36404954-36404973 | None:intergenic | 40.0% | |
| TAAAGAAAACGCTTGGAGCT+TGG | + | chr5.1:36405032-36405051 | None:intergenic | 40.0% | |
| TCTCCATCTTGTTCATGCTT+TGG | - | chr5.1:36404955-36404974 | MS.gene62140:CDS | 40.0% | |
| ! | GTTTCTTTTCAGCAGCAGAT+AGG | - | chr5.1:36405124-36405143 | MS.gene62140:CDS | 40.0% |
| !! | AATAGCTTTGAATTACCGGC+TGG | + | chr5.1:36404982-36405001 | None:intergenic | 40.0% |
| !! | GCATGAGTTGGTGGTAATTA+AGG | + | chr5.1:36405739-36405758 | None:intergenic | 40.0% |
| AACCAGACAGAAGCAGTTGA+TGG | + | chr5.1:36405163-36405182 | None:intergenic | 45.0% | |
| CATAGCAGGGACACATTTCT+TGG | + | chr5.1:36405655-36405674 | None:intergenic | 45.0% | |
| GGTCCAAAGCATGAACAAGA+TGG | + | chr5.1:36404961-36404980 | None:intergenic | 45.0% | |
| TCTTGGTTACCAGAAGTTCC+TGG | + | chr5.1:36405638-36405657 | None:intergenic | 45.0% | |
| TGAGTTTCTGCGTGTACATG+CGG | + | chr5.1:36405103-36405122 | None:intergenic | 45.0% | |
| TTCCATCAACTGCTTCTGTC+TGG | - | chr5.1:36405158-36405177 | MS.gene62140:intron | 45.0% | |
| ! | AGTTGCATTGCATGAGTTGG+TGG | + | chr5.1:36405748-36405767 | None:intergenic | 45.0% |
| !! | CTAGGTGCCGTTTATCATCA+CGG | - | chr5.1:36405555-36405574 | MS.gene62140:intron | 45.0% |
| !! | GTAATTAAATTAAATTAAAT+TGG | + | chr5.1:36405063-36405082 | None:intergenic | 5.0% |
| GCAAGTGCCCTTGAATTGAG+TGG | - | chr5.1:36405702-36405721 | MS.gene62140:intron | 50.0% | |
| TATGCCAACCAAACCACCCA+TGG | - | chr5.1:36405671-36405690 | MS.gene62140:intron | 50.0% | |
| TGGTTACCAGAAGTTCCTGG+TGG | + | chr5.1:36405635-36405654 | None:intergenic | 50.0% | |
| ! | TGTTCATGCTTTGGACCAGC+CGG | - | chr5.1:36404964-36404983 | MS.gene62140:CDS | 50.0% |
| !! | CATGTGGAACTTGCTGCAGT+CGG | - | chr5.1:36405597-36405616 | MS.gene62140:intron | 50.0% |
| !! | GTGGTTTGGTTGGCATAGCA+GGG | + | chr5.1:36405668-36405687 | None:intergenic | 50.0% |
| AGCAAGTTCCACATGCCCTG+TGG | + | chr5.1:36405592-36405611 | None:intergenic | 55.0% | |
| ! | GCAATGGAGCATGTGCTGCT+AGG | - | chr5.1:36405537-36405556 | MS.gene62140:intron | 55.0% |
| !! | GGTGGTTTGGTTGGCATAGC+AGG | + | chr5.1:36405669-36405688 | None:intergenic | 55.0% |
| GCACTTGCGTCTGCCTCCAT+GGG | + | chr5.1:36405690-36405709 | None:intergenic | 60.0% | |
| GCCAACCAAACCACCCATGG+AGG | - | chr5.1:36405674-36405693 | MS.gene62140:intron | 60.0% | |
| GGCGCGGCCGTGATGATAAA+CGG | + | chr5.1:36405565-36405584 | None:intergenic | 60.0% | |
| GTCTGCCTCCATGGGTGGTT+TGG | + | chr5.1:36405682-36405701 | None:intergenic | 60.0% | |
| TGCGTGCCACCAGGAACTTC+TGG | - | chr5.1:36405626-36405645 | MS.gene62140:intron | 60.0% | |
| TTCCACATGCCCTGTGGCAG+AGG | + | chr5.1:36405586-36405605 | None:intergenic | 60.0% | |
| !! | GCCTCCATGGGTGGTTTGGT+TGG | + | chr5.1:36405678-36405697 | None:intergenic | 60.0% |
| CTTGCGTCTGCCTCCATGGG+TGG | + | chr5.1:36405687-36405706 | None:intergenic | 65.0% | |
| GGCACTTGCGTCTGCCTCCA+TGG | + | chr5.1:36405691-36405710 | None:intergenic | 65.0% | |
| CATGCCCTGTGGCAGAGGCG+CGG | + | chr5.1:36405581-36405600 | None:intergenic | 70.0% | |
| CGCCTCTGCCACAGGGCATG+TGG | - | chr5.1:36405581-36405600 | MS.gene62140:intron | 70.0% | |
| CGGTGCAACTGCGTGCCACC+AGG | - | chr5.1:36405617-36405636 | MS.gene62140:intron | 70.0% | |
| ACGGCCGCGCCTCTGCCACA+GGG | - | chr5.1:36405574-36405593 | MS.gene62140:intron | 75.0% | |
| ! | CACGGCCGCGCCTCTGCCAC+AGG | - | chr5.1:36405573-36405592 | MS.gene62140:intron | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 36404905 | 36405791 | 36404905 | ID=MS.gene62140 |
| chr5.1 | mRNA | 36404905 | 36405791 | 36404905 | ID=MS.gene62140.t1;Parent=MS.gene62140 |
| chr5.1 | exon | 36405711 | 36405791 | 36405711 | ID=MS.gene62140.t1.exon1;Parent=MS.gene62140.t1 |
| chr5.1 | CDS | 36405711 | 36405791 | 36405711 | ID=cds.MS.gene62140.t1;Parent=MS.gene62140.t1 |
| chr5.1 | exon | 36404905 | 36405162 | 36404905 | ID=MS.gene62140.t1.exon2;Parent=MS.gene62140.t1 |
| chr5.1 | CDS | 36404905 | 36405162 | 36404905 | ID=cds.MS.gene62140.t1;Parent=MS.gene62140.t1 |
| Gene Sequence |
| Protein sequence |
