Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62825.t1 | XP_013457650.1 | 95.5 | 154 | 7 | 0 | 1 | 154 | 119 | 272 | 2.00E-68 | 268.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62825.t1 | Q50EX6 | 42.1 | 178 | 76 | 5 | 1 | 154 | 119 | 293 | 4.8e-15 | 82.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62825.t1 | A0A072UQP4 | 95.5 | 154 | 7 | 0 | 1 | 154 | 119 | 272 | 1.4e-68 | 268.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049227 | MS.gene62825 | 0.813282 | 2.77E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62825.t1 | MTR_4g102380 | 97.403 | 154 | 4 | 0 | 1 | 154 | 119 | 272 | 1.25e-106 | 305 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62825.t1 | AT4G22680 | 37.838 | 148 | 77 | 5 | 1 | 133 | 119 | 266 | 2.15e-13 | 65.9 |
| MS.gene62825.t1 | AT4G12350 | 33.333 | 168 | 86 | 8 | 1 | 146 | 119 | 282 | 4.06e-13 | 65.5 |
Find 30 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAGTTTCCTTCACTTCA+TGG | 0.233477 | 8.1:-21280597 | None:intergenic |
| TTCACTTCATGGTTGTTAAC+AGG | 0.301922 | 8.1:-21280586 | None:intergenic |
| TCCCCAGCTGAAAATTCTTC+TGG | 0.318088 | 8.1:+21280652 | MS.gene62825:CDS |
| ACCATTGGTGGAAGCCCTTT+GGG | 0.330830 | 8.1:+21280747 | MS.gene62825:CDS |
| TTCAAAGGTTCATGAGTAAT+GGG | 0.340405 | 8.1:-21280505 | None:intergenic |
| CACCATTGGTGGAAGCCCTT+TGG | 0.357777 | 8.1:+21280746 | MS.gene62825:CDS |
| TCAAGTACACTGCAAACTAT+AGG | 0.364172 | 8.1:+21280913 | MS.gene62825:CDS |
| ATTCTCTAATGAATAGTATA+TGG | 0.364682 | 8.1:+21280713 | MS.gene62825:CDS |
| TGCTTGGCTATTTGATTGTC+AGG | 0.369569 | 8.1:+21280843 | MS.gene62825:CDS |
| GGTGGAAGCCCTTTGGGAAA+TGG | 0.381456 | 8.1:+21280753 | MS.gene62825:CDS |
| GACTTTGGTATTCATGACTT+TGG | 0.387701 | 8.1:+21280865 | MS.gene62825:CDS |
| CTATTTGATTGTCAGGACTT+TGG | 0.417724 | 8.1:+21280850 | MS.gene62825:CDS |
| ATTCAAAGGTTCATGAGTAA+TGG | 0.432527 | 8.1:-21280506 | None:intergenic |
| CAAGTACACTGCAAACTATA+GGG | 0.456076 | 8.1:+21280914 | MS.gene62825:CDS |
| GATGATGCTTGTTTATTCAA+AGG | 0.463222 | 8.1:-21280520 | None:intergenic |
| GGATGAAGTACTACTATCAT+TGG | 0.464276 | 8.1:-21280542 | None:intergenic |
| CTTGGGAGGATAATTGTGCT+TGG | 0.466595 | 8.1:+21280827 | MS.gene62825:CDS |
| TCCCAAAGGGCTTCCACCAA+TGG | 0.485135 | 8.1:-21280748 | None:intergenic |
| TGTAGTGTCCATTTCCCAAA+GGG | 0.498177 | 8.1:-21280761 | None:intergenic |
| TCTAATGAATAGTATATGGT+TGG | 0.521314 | 8.1:+21280717 | MS.gene62825:CDS |
| ACTGCAAACTATAGGGATGA+AGG | 0.532537 | 8.1:+21280921 | MS.gene62825:CDS |
| ATGAAGTGAAGGAAACTGAT+GGG | 0.557455 | 8.1:+21280599 | MS.gene62825:CDS |
| CATGAAGTGAAGGAAACTGA+TGG | 0.577027 | 8.1:+21280598 | MS.gene62825:CDS |
| GCACAATTATCCTCCCAAGA+AGG | 0.588656 | 8.1:-21280823 | None:intergenic |
| TGTTAACAACCATGAAGTGA+AGG | 0.612004 | 8.1:+21280588 | MS.gene62825:CDS |
| GTTGGATGAAACACCATTGG+TGG | 0.620513 | 8.1:+21280735 | MS.gene62825:CDS |
| GTGTAGTGTCCATTTCCCAA+AGG | 0.630743 | 8.1:-21280762 | None:intergenic |
| ATGGTTGGATGAAACACCAT+TGG | 0.670766 | 8.1:+21280732 | MS.gene62825:CDS |
| TGATGGGGTTGTCAACTCAG+AGG | 0.704259 | 8.1:+21280615 | MS.gene62825:CDS |
| TGAAGTGAAGGAAACTGATG+GGG | 0.739601 | 8.1:+21280600 | MS.gene62825:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTCTAATGAATAGTATA+TGG | + | chr8.1:21280713-21280732 | MS.gene62825:CDS | 20.0% |
| ! | TCTAATGAATAGTATATGGT+TGG | + | chr8.1:21280717-21280736 | MS.gene62825:CDS | 25.0% |
| !!! | TTTTTTGTGTTTTCTGCTAT+AGG | - | chr8.1:21280787-21280806 | None:intergenic | 25.0% |
| ATTCAAAGGTTCATGAGTAA+TGG | - | chr8.1:21280509-21280528 | None:intergenic | 30.0% | |
| GATGATGCTTGTTTATTCAA+AGG | - | chr8.1:21280523-21280542 | None:intergenic | 30.0% | |
| TTCAAAGGTTCATGAGTAAT+GGG | - | chr8.1:21280508-21280527 | None:intergenic | 30.0% | |
| ATGAAGTGAAGGAAACTGAT+GGG | + | chr8.1:21280599-21280618 | MS.gene62825:CDS | 35.0% | |
| CAAGTACACTGCAAACTATA+GGG | + | chr8.1:21280914-21280933 | MS.gene62825:CDS | 35.0% | |
| CTATTTGATTGTCAGGACTT+TGG | + | chr8.1:21280850-21280869 | MS.gene62825:CDS | 35.0% | |
| GACTTTGGTATTCATGACTT+TGG | + | chr8.1:21280865-21280884 | MS.gene62825:CDS | 35.0% | |
| TCAAGTACACTGCAAACTAT+AGG | + | chr8.1:21280913-21280932 | MS.gene62825:CDS | 35.0% | |
| TGTTAACAACCATGAAGTGA+AGG | + | chr8.1:21280588-21280607 | MS.gene62825:CDS | 35.0% | |
| TTCACTTCATGGTTGTTAAC+AGG | - | chr8.1:21280589-21280608 | None:intergenic | 35.0% | |
| ! | GGATGAAGTACTACTATCAT+TGG | - | chr8.1:21280545-21280564 | None:intergenic | 35.0% |
| !! | ACATGAGTTTTATGCCTTCT+TGG | + | chr8.1:21280809-21280828 | MS.gene62825:CDS | 35.0% |
| !! | CATGAGTTTTATGCCTTCTT+GGG | + | chr8.1:21280810-21280829 | MS.gene62825:CDS | 35.0% |
| ACTGCAAACTATAGGGATGA+AGG | + | chr8.1:21280921-21280940 | MS.gene62825:CDS | 40.0% | |
| ATGGTTGGATGAAACACCAT+TGG | + | chr8.1:21280732-21280751 | MS.gene62825:CDS | 40.0% | |
| CATCAGTTTCCTTCACTTCA+TGG | - | chr8.1:21280600-21280619 | None:intergenic | 40.0% | |
| CATGAAGTGAAGGAAACTGA+TGG | + | chr8.1:21280598-21280617 | MS.gene62825:CDS | 40.0% | |
| TGAAGTGAAGGAAACTGATG+GGG | + | chr8.1:21280600-21280619 | MS.gene62825:CDS | 40.0% | |
| TGCTTGGCTATTTGATTGTC+AGG | + | chr8.1:21280843-21280862 | MS.gene62825:CDS | 40.0% | |
| TGTAGTGTCCATTTCCCAAA+GGG | - | chr8.1:21280764-21280783 | None:intergenic | 40.0% | |
| ! | CTCCAGAAGAATTTTCAGCT+GGG | - | chr8.1:21280657-21280676 | None:intergenic | 40.0% |
| ! | TCCAGAAGAATTTTCAGCTG+GGG | - | chr8.1:21280656-21280675 | None:intergenic | 40.0% |
| ! | TCTCCAGAAGAATTTTCAGC+TGG | - | chr8.1:21280658-21280677 | None:intergenic | 40.0% |
| CTTGGGAGGATAATTGTGCT+TGG | + | chr8.1:21280827-21280846 | MS.gene62825:CDS | 45.0% | |
| GCACAATTATCCTCCCAAGA+AGG | - | chr8.1:21280826-21280845 | None:intergenic | 45.0% | |
| GTGTAGTGTCCATTTCCCAA+AGG | - | chr8.1:21280765-21280784 | None:intergenic | 45.0% | |
| GTTGGATGAAACACCATTGG+TGG | + | chr8.1:21280735-21280754 | MS.gene62825:CDS | 45.0% | |
| TCCCCAGCTGAAAATTCTTC+TGG | + | chr8.1:21280652-21280671 | MS.gene62825:CDS | 45.0% | |
| ! | CTGTGACGAATTTTCTGCAG+GGG | - | chr8.1:21280566-21280585 | None:intergenic | 45.0% |
| ! | GCTGTGACGAATTTTCTGCA+GGG | - | chr8.1:21280567-21280586 | None:intergenic | 45.0% |
| !! | GAGTTTTATGCCTTCTTGGG+AGG | + | chr8.1:21280813-21280832 | MS.gene62825:CDS | 45.0% |
| ACCATTGGTGGAAGCCCTTT+GGG | + | chr8.1:21280747-21280766 | MS.gene62825:CDS | 50.0% | |
| TGATGGGGTTGTCAACTCAG+AGG | + | chr8.1:21280615-21280634 | MS.gene62825:CDS | 50.0% | |
| ! | GGCTGTGACGAATTTTCTGC+AGG | - | chr8.1:21280568-21280587 | None:intergenic | 50.0% |
| CACCATTGGTGGAAGCCCTT+TGG | + | chr8.1:21280746-21280765 | MS.gene62825:CDS | 55.0% | |
| TCCCAAAGGGCTTCCACCAA+TGG | - | chr8.1:21280751-21280770 | None:intergenic | 55.0% | |
| ! | GGTGGAAGCCCTTTGGGAAA+TGG | + | chr8.1:21280753-21280772 | MS.gene62825:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 21280493 | 21280957 | 21280493 | ID=MS.gene62825 |
| chr8.1 | mRNA | 21280493 | 21280957 | 21280493 | ID=MS.gene62825.t1;Parent=MS.gene62825 |
| chr8.1 | exon | 21280493 | 21280957 | 21280493 | ID=MS.gene62825.t1.exon1;Parent=MS.gene62825.t1 |
| chr8.1 | CDS | 21280493 | 21280957 | 21280493 | ID=cds.MS.gene62825.t1;Parent=MS.gene62825.t1 |
| Gene Sequence |
| Protein sequence |
