Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63193.t1 | RHN80032.1 | 89.9 | 99 | 10 | 0 | 1 | 99 | 48 | 146 | 4.30E-40 | 173.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63193.t1 | Q6Q1P4 | 70.7 | 99 | 29 | 0 | 1 | 99 | 49 | 147 | 1.3e-32 | 140.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63193.t1 | A0A072VWQ4 | 89.9 | 99 | 10 | 0 | 1 | 99 | 48 | 146 | 3.1e-40 | 173.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058010 | MS.gene63193 | 0.808712 | 2.71E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63193.t1 | MTR_1g069910 | 89.899 | 99 | 10 | 0 | 1 | 99 | 48 | 146 | 1.29e-55 | 185 |
| MS.gene63193.t1 | MTR_0003s0460 | 68.687 | 99 | 29 | 1 | 1 | 99 | 44 | 140 | 9.00e-37 | 131 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63193.t1 | AT3G54670 | 70.707 | 99 | 29 | 0 | 1 | 99 | 49 | 147 | 1.81e-40 | 142 |
Find 17 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGATGATAGCGTTGTTAAT+TGG | 0.242553 | 8.4:+41183382 | MS.gene63193:CDS |
| TGTCAAAGCACGCAATTTCT+TGG | 0.279718 | 8.4:+41183443 | MS.gene63193:CDS |
| AGTTTGAATGAATTTGATTT+CGG | 0.301055 | 8.4:-41183328 | None:intergenic |
| TCGAAGAGCGTTTGTGAGAT+TGG | 0.348127 | 8.4:+41183284 | MS.gene63193:CDS |
| TGATGATAGCGTTGTTAATT+GGG | 0.389204 | 8.4:+41183383 | MS.gene63193:CDS |
| CTGCTGGTGCTAGTGAGTAT+TGG | 0.435103 | 8.4:+41183358 | MS.gene63193:CDS |
| ATTCAAACTATTATTGCTGC+TGG | 0.448325 | 8.4:+41183342 | MS.gene63193:CDS |
| GATGCGAGACTCAAATCGTT+GGG | 0.451354 | 8.4:+41183414 | MS.gene63193:CDS |
| CTGAGCGCCACGGAGTTGAT+TGG | 0.496915 | 8.4:-41183211 | None:intergenic |
| TCTGTTCTTTCTCATGATCG+TGG | 0.537868 | 8.4:-41183257 | None:intergenic |
| CGATGCGAGACTCAAATCGT+TGG | 0.551346 | 8.4:+41183413 | MS.gene63193:CDS |
| GACGCTATCAGCTTCGTCCT+CGG | 0.579751 | 8.4:+41183180 | MS.gene63193:CDS |
| GATCATGAGAAAGAACAGAA+AGG | 0.651059 | 8.4:+41183261 | MS.gene63193:CDS |
| GATCTTTCAACTGAGCGCCA+CGG | 0.655874 | 8.4:-41183221 | None:intergenic |
| GTTGATTGGTGCGAACGCCG+AGG | 0.670333 | 8.4:-41183197 | None:intergenic |
| GTTCGCACCAATCAACTCCG+TGG | 0.680023 | 8.4:+41183204 | MS.gene63193:CDS |
| ATGCGAGACTCAAATCGTTG+GGG | 0.681702 | 8.4:+41183415 | MS.gene63193:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTGAATGAATTTGATTT+CGG | - | chr8.4:41183331-41183350 | None:intergenic | 20.0% |
| ATTCAAACTATTATTGCTGC+TGG | + | chr8.4:41183342-41183361 | MS.gene63193:CDS | 30.0% | |
| ! | TGATGATAGCGTTGTTAATT+GGG | + | chr8.4:41183383-41183402 | MS.gene63193:CDS | 30.0% |
| ! | TTGATGATAGCGTTGTTAAT+TGG | + | chr8.4:41183382-41183401 | MS.gene63193:CDS | 30.0% |
| GATCATGAGAAAGAACAGAA+AGG | + | chr8.4:41183261-41183280 | MS.gene63193:CDS | 35.0% | |
| !! | ACGCAATTTCTTGGTTTTTC+AGG | + | chr8.4:41183452-41183471 | MS.gene63193:CDS | 35.0% |
| !! | CGCAATTTCTTGGTTTTTCA+GGG | + | chr8.4:41183453-41183472 | MS.gene63193:CDS | 35.0% |
| TCTGTTCTTTCTCATGATCG+TGG | - | chr8.4:41183260-41183279 | None:intergenic | 40.0% | |
| TGTCAAAGCACGCAATTTCT+TGG | + | chr8.4:41183443-41183462 | MS.gene63193:CDS | 40.0% | |
| AATTGCGTCGAAGAATCCGA+AGG | + | chr8.4:41183488-41183507 | MS.gene63193:intron | 45.0% | |
| ATGCGAGACTCAAATCGTTG+GGG | + | chr8.4:41183415-41183434 | MS.gene63193:CDS | 45.0% | |
| GATGCGAGACTCAAATCGTT+GGG | + | chr8.4:41183414-41183433 | MS.gene63193:CDS | 45.0% | |
| GGGCTTATTGAGCAGATATC+TGG | + | chr8.4:41183519-41183538 | MS.gene63193:intron | 45.0% | |
| !! | TCGAAGAGCGTTTGTGAGAT+TGG | + | chr8.4:41183284-41183303 | MS.gene63193:CDS | 45.0% |
| !!! | CTTGGTTTTTCAGGGTGATG+TGG | + | chr8.4:41183461-41183480 | MS.gene63193:intron | 45.0% |
| AAGAATCCGAAGGAGCTCAC+TGG | + | chr8.4:41183498-41183517 | MS.gene63193:intron | 50.0% | |
| AGAATCCGAAGGAGCTCACT+GGG | + | chr8.4:41183499-41183518 | MS.gene63193:intron | 50.0% | |
| ATAAGCCCAGTGAGCTCCTT+CGG | - | chr8.4:41183507-41183526 | None:intergenic | 50.0% | |
| CGATGCGAGACTCAAATCGT+TGG | + | chr8.4:41183413-41183432 | MS.gene63193:CDS | 50.0% | |
| GATCTTTCAACTGAGCGCCA+CGG | - | chr8.4:41183224-41183243 | None:intergenic | 50.0% | |
| ! | CTGCTGGTGCTAGTGAGTAT+TGG | + | chr8.4:41183358-41183377 | MS.gene63193:CDS | 50.0% |
| GACGCTATCAGCTTCGTCCT+CGG | + | chr8.4:41183180-41183199 | MS.gene63193:CDS | 55.0% | |
| GTTCGCACCAATCAACTCCG+TGG | + | chr8.4:41183204-41183223 | MS.gene63193:CDS | 55.0% | |
| GTTGATTGGTGCGAACGCCG+AGG | - | chr8.4:41183200-41183219 | None:intergenic | 60.0% | |
| ! | CTGAGCGCCACGGAGTTGAT+TGG | - | chr8.4:41183214-41183233 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 41183177 | 41183577 | 41183177 | ID=MS.gene63193 |
| chr8.4 | mRNA | 41183177 | 41183577 | 41183177 | ID=MS.gene63193.t1;Parent=MS.gene63193 |
| chr8.4 | exon | 41183177 | 41183474 | 41183177 | ID=MS.gene63193.t1.exon1;Parent=MS.gene63193.t1 |
| chr8.4 | CDS | 41183177 | 41183474 | 41183177 | ID=cds.MS.gene63193.t1;Parent=MS.gene63193.t1 |
| chr8.4 | exon | 41183573 | 41183577 | 41183573 | ID=MS.gene63193.t1.exon2;Parent=MS.gene63193.t1 |
| chr8.4 | CDS | 41183573 | 41183577 | 41183573 | ID=cds.MS.gene63193.t1;Parent=MS.gene63193.t1 |
| Gene Sequence |
| Protein sequence |
