AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63549

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63549.t1 MTR_1g034960 98.298 235 4 0 1 235 79 313 6.56e-175 483
MS.gene63549.t1 MTR_1g034390 30.769 156 91 4 8 163 43 181 8.71e-19 82.8
MS.gene63549.t1 MTR_4g068790 30.769 156 90 4 8 163 25 162 7.39e-17 77.4
MS.gene63549.t1 MTR_7g025000 32.394 142 73 6 8 144 16 139 7.28e-14 68.6
MS.gene63549.t1 MTR_7g024930 34.234 111 66 3 8 116 17 122 4.84e-13 66.2
MS.gene63549.t1 MTR_7g024950 35.417 96 57 3 8 101 19 111 5.42e-13 66.6
MS.gene63549.t1 MTR_7g093330 28.025 157 97 4 10 159 33 180 5.62e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63549.t1 AT1G20990 52.743 237 106 5 2 234 81 315 1.58e-79 241
MS.gene63549.t1 AT2G37805 32.955 176 99 7 2 171 23 185 4.91e-21 89.0
MS.gene63549.t1 AT2G37820 35.065 154 82 7 8 158 29 167 1.24e-20 88.6
MS.gene63549.t1 AT2G37800 32.121 165 97 5 2 164 23 174 1.21e-19 85.1
MS.gene63549.t1 AT2G16050 31.288 163 85 5 8 163 25 167 8.77e-18 79.7
MS.gene63549.t1 AT2G42060 32.164 171 103 6 9 170 28 194 4.30e-17 78.6
MS.gene63549.t1 AT2G44380 32.051 156 91 6 6 155 30 176 2.08e-16 76.3
MS.gene63549.t1 AT2G28270 30.723 166 102 7 6 165 29 187 2.98e-15 73.2
MS.gene63549.t1 AT2G37810 30.864 162 91 6 2 161 23 165 3.99e-15 72.4
MS.gene63549.t1 AT2G27660 30.921 152 93 7 10 158 32 174 1.17e-14 73.2
MS.gene63549.t1 AT5G22355 29.710 138 86 4 26 161 102 230 7.77e-13 67.8
MS.gene63549.t1 AT2G44370 31.373 153 89 6 8 155 28 169 2.77e-11 62.0

Find 60 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGCTAAGAATAAAGTTAA+TGG 0.247808 1.4:-19533497 MS.gene63549:CDS
CAAGTTGTGATTGAATTTAT+TGG 0.281922 1.4:-19533404 MS.gene63549:CDS
TGAAGGTATTGGTGAAAGTA+TGG 0.294851 1.4:-19533372 MS.gene63549:CDS
TAGAGTTCCATTTCCTCCTT+TGG 0.301378 1.4:+19533325 None:intergenic
TTTGGCTCCTCCTGCCCTTA+AGG 0.321453 1.4:-19535549 MS.gene63549:CDS
GTCTTCAAGCTAATGGTATT+AGG 0.329280 1.4:-19533475 MS.gene63549:CDS
TTGAGAGTGTGTGGATGATT+TGG 0.350556 1.4:+19533659 None:intergenic
GGTGGGTTTGTTGAAGGTAT+TGG 0.358236 1.4:-19533383 MS.gene63549:CDS
AATATTACCAAAGGAGGAAA+TGG 0.372011 1.4:-19533332 MS.gene63549:CDS
TTTGCCACAAACGTCGCATT+TGG 0.379974 1.4:+19533769 None:intergenic
CTGACCTCTTTATTTGTGTA+GGG 0.381966 1.4:-19535643 MS.gene63549:CDS
ATCGGTGTTTGTTGCGGCCC+CGG 0.388093 1.4:+19533613 None:intergenic
TGAGAGTGTGTGGATGATTT+GGG 0.389020 1.4:+19533660 None:intergenic
GTTAATGGTCTTCAAGCTAA+TGG 0.394008 1.4:-19533482 MS.gene63549:CDS
GAGATAGTTAGTACCTGGTT+TGG 0.409720 1.4:+19535487 None:intergenic
CCTGACCTCTTTATTTGTGT+AGG 0.416136 1.4:-19535644 MS.gene63549:CDS
TGGCACAACATGGATGCATT+TGG 0.423995 1.4:+19533705 None:intergenic
ATGGAAAGGATGAGCCTTAA+GGG 0.427154 1.4:+19535535 None:intergenic
AATGGAAAGGATGAGCCTTA+AGG 0.437935 1.4:+19535534 None:intergenic
ATGGAACAACATTGCATGTT+GGG 0.440574 1.4:+19535511 None:intergenic
TGTGTAGGGTGTAAAGAATA+TGG 0.442579 1.4:-19535629 MS.gene63549:CDS
AAATCCAAATGCGACGTTTG+TGG 0.443404 1.4:-19533773 MS.gene63549:CDS
TTATATATTTCAGTGAAAAG+TGG 0.456980 1.4:-19533803 MS.gene63549:intron
GAGTGCAAGAAACGAAGGTC+GGG 0.467602 1.4:-19533566 MS.gene63549:CDS
AGTAGTATTATTATTAGCAC+TGG 0.474464 1.4:+19533301 None:intergenic
TTCAATTTCTGTGTTGAGCA+TGG 0.482378 1.4:+19533685 None:intergenic
GTTTGTTGCGGCCCCGGTGG+TGG 0.489625 1.4:+19533619 None:intergenic
TTTATTGGTGGGTTTGTTGA+AGG 0.497338 1.4:-19533389 MS.gene63549:CDS
CACATTCAACAACCACCACC+GGG 0.505441 1.4:-19533631 MS.gene63549:CDS
AGTTAGTACCTGGTTTGGAA+TGG 0.516387 1.4:+19535492 None:intergenic
CTTGTTCAAAATATTACCAA+AGG 0.523980 1.4:-19533341 MS.gene63549:CDS
AACGATCGTGTTGGAAAGAT+CGG 0.526444 1.4:+19533595 None:intergenic
ACACATTCAACAACCACCAC+CGG 0.526885 1.4:-19533632 MS.gene63549:CDS
ACAACATTGCATGTTGGGAA+TGG 0.533156 1.4:+19535516 None:intergenic
GGGTGTAAAGAATATGGCTC+TGG 0.542115 1.4:-19535623 MS.gene63549:CDS
GTTTGTGGCAAACCAACAAA+AGG 0.544375 1.4:-19533758 MS.gene63549:CDS
TACACCCTACACAAATAAAG+AGG 0.550185 1.4:+19535639 None:intergenic
CCTACACAAATAAAGAGGTC+AGG 0.555538 1.4:+19535644 None:intergenic
ATGTTGTTCCATTCCAAACC+AGG 0.562519 1.4:-19535500 MS.gene63549:intron
GTTCAAAATATTACCAAAGG+AGG 0.564880 1.4:-19533338 MS.gene63549:CDS
AAAGGATGAGCCTTAAGGGC+AGG 0.575481 1.4:+19535539 None:intergenic
GCAGCAGCAGAAAGAACACT+TGG 0.575529 1.4:+19533440 None:intergenic
CTCGTTGCAAACGATCGTGT+TGG 0.577724 1.4:+19533586 None:intergenic
GGAAAGATCGGTGTTTGTTG+CGG 0.583663 1.4:+19533607 None:intergenic
TGTGTAAGTTTGAGAGTGTG+TGG 0.589605 1.4:+19533650 None:intergenic
CACAAATAAAGAGGTCAGGA+AGG 0.591951 1.4:+19535648 None:intergenic
AATGGAACAACATTGCATGT+TGG 0.602139 1.4:+19535510 None:intergenic
GAAGGTATTGGTGAAAGTAT+GGG 0.605206 1.4:-19533371 MS.gene63549:CDS
GTTGTGATTGAATTTATTGG+TGG 0.605324 1.4:-19533401 MS.gene63549:CDS
GGGGAAAGTGTATCGGTGCA+CGG 0.605648 1.4:-19533546 MS.gene63549:CDS
TTGTGATTGAATTTATTGGT+GGG 0.605849 1.4:-19533400 MS.gene63549:CDS
GGATGAGCCTTAAGGGCAGG+AGG 0.620081 1.4:+19535542 None:intergenic
GAAGGTCGGGGAAAGTGTAT+CGG 0.622440 1.4:-19533553 MS.gene63549:CDS
CGAGTGCAAGAAACGAAGGT+CGG 0.625441 1.4:-19533567 MS.gene63549:CDS
GGTGTTTGTTGCGGCCCCGG+TGG 0.636946 1.4:+19533616 None:intergenic
AGTGCAAGAAACGAAGGTCG+GGG 0.650929 1.4:-19533565 MS.gene63549:CDS
GTGTTGAGCATGGCACAACA+TGG 0.659309 1.4:+19533695 None:intergenic
GCAACGAGTGCAAGAAACGA+AGG 0.666806 1.4:-19533571 MS.gene63549:CDS
ATTGCATGTTGGGAATGGAA+AGG 0.699207 1.4:+19535521 None:intergenic
ACATTCAACAACCACCACCG+GGG 0.748062 1.4:-19533630 MS.gene63549:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATAATAAATAGGAAA+TGG - chr1.4:19534815-19534834 MS.gene63549:intron 10.0%
!! CAATAATAATAAAATATAGA+TGG - chr1.4:19534393-19534412 MS.gene63549:intron 10.0%
!!! CTATATTTTATTATTATTGA+TGG + chr1.4:19534392-19534411 None:intergenic 10.0%
!!! TATACTAATTTTAACTAATA+CGG - chr1.4:19534234-19534253 MS.gene63549:intron 10.0%
!!! TCCTATTTATTATATTTTAA+AGG + chr1.4:19534813-19534832 None:intergenic 10.0%
!! ATTTACAAAAAATAAAACTG+AGG + chr1.4:19533999-19534018 None:intergenic 15.0%
!! CAAATAATTCAACTATTAAT+TGG + chr1.4:19533489-19533508 None:intergenic 15.0%
!! CTATTAATTGGTAAAATTTA+TGG + chr1.4:19533477-19533496 None:intergenic 15.0%
!! GAAAAGTTGAAATATTTATA+GGG + chr1.4:19534954-19534973 None:intergenic 15.0%
!! GTAAATAAAATGGAAAATAT+TGG + chr1.4:19534022-19534041 None:intergenic 15.0%
!! TGAAAAGTTGAAATATTTAT+AGG + chr1.4:19534955-19534974 None:intergenic 15.0%
!! TGCATAAAAAGTAAATAAAA+TGG + chr1.4:19534032-19534051 None:intergenic 15.0%
!! TTTAAATGAACCAATTTAAT+AGG + chr1.4:19533794-19533813 None:intergenic 15.0%
!! TTTATATGTCTATAAGTAAT+CGG - chr1.4:19533859-19533878 MS.gene63549:intron 15.0%
!!! GCCTTTAAAATATAATAAAT+AGG - chr1.4:19534809-19534828 MS.gene63549:intron 15.0%
!!! TATTTTATTATTATTGATGG+TGG + chr1.4:19534389-19534408 None:intergenic 15.0%
!!! TTAAATTGGTTCATTTAAAT+CGG - chr1.4:19533795-19533814 MS.gene63549:intron 15.0%
!!! TTTTATTTACTTTTTATGCA+TGG - chr1.4:19534032-19534051 MS.gene63549:intron 15.0%
!! GAAAATGTTTACAAACTTAA+AGG + chr1.4:19534575-19534594 None:intergenic 20.0%
!! TATTTAATGCCACAATATAT+GGG + chr1.4:19533964-19533983 None:intergenic 20.0%
!! TTAGCGAATCAAAATAAATA+TGG - chr1.4:19534886-19534905 MS.gene63549:intron 20.0%
!! TTATATATTTCAGTGAAAAG+TGG - chr1.4:19535136-19535155 MS.gene63549:intron 20.0%
!! TTGTTTCATAGTTTCTAAAT+CGG - chr1.4:19533760-19533779 MS.gene63549:CDS 20.0%
!!! AAAAATTTCATCTTCACTTT+TGG - chr1.4:19535018-19535037 MS.gene63549:intron 20.0%
!!! AAGTGAAGATGAAATTTTTT+TGG + chr1.4:19535017-19535036 None:intergenic 20.0%
!!! AATGATCCTTTAAGTTATTT+TGG - chr1.4:19534537-19534556 MS.gene63549:intron 20.0%
!!! ATTTGAGTTTACTTTAAATG+AGG + chr1.4:19535046-19535065 None:intergenic 20.0%
!!! TTTGAGTTTACTTTAAATGA+GGG + chr1.4:19535045-19535064 None:intergenic 20.0%
!!! TTTTTTTTGTTTTTGTGATG+TGG - chr1.4:19534135-19534154 MS.gene63549:intron 20.0%
! AAGTTAACCAAACAATGATA+TGG + chr1.4:19534617-19534636 None:intergenic 25.0%
! AGTAGTATTATTATTAGCAC+TGG + chr1.4:19535641-19535660 None:intergenic 25.0%
! ATATGCAATGTTAAAGAGTA+GGG + chr1.4:19535078-19535097 None:intergenic 25.0%
! ATCATTGTTTGGTTAACTTA+AGG - chr1.4:19534618-19534637 MS.gene63549:intron 25.0%
! ATTAGATGAAATTGTGACAT+GGG + chr1.4:19534359-19534378 None:intergenic 25.0%
! ATTTAATGCCACAATATATG+GGG + chr1.4:19533963-19533982 None:intergenic 25.0%
! CAAGTTGTGATTGAATTTAT+TGG - chr1.4:19535535-19535554 MS.gene63549:CDS 25.0%
! CATATATTGTGGCATTAAAT+AGG - chr1.4:19533963-19533982 MS.gene63549:intron 25.0%
! CTATTTAATGCCACAATATA+TGG + chr1.4:19533965-19533984 None:intergenic 25.0%
! CTTGTTCAAAATATTACCAA+AGG - chr1.4:19535598-19535617 MS.gene63549:CDS 25.0%
! GAAAACGTTTACAAACTTAA+AGG - chr1.4:19534649-19534668 MS.gene63549:intron 25.0%
! GGTAAAATCACCTATTAAAT+TGG - chr1.4:19533781-19533800 MS.gene63549:CDS 25.0%
! GTTACAAATCTCAAACTTAA+AGG - chr1.4:19534709-19534728 MS.gene63549:intron 25.0%
! TCTTGATTTCATTCTCATTA+TGG - chr1.4:19535101-19535120 MS.gene63549:intron 25.0%
! TGTGCTAAGAATAAAGTTAA+TGG - chr1.4:19535442-19535461 MS.gene63549:intron 25.0%
! TTGTGATTGAATTTATTGGT+GGG - chr1.4:19535539-19535558 MS.gene63549:CDS 25.0%
!! ATAGGAAATGGAAGATTTTA+TGG - chr1.4:19534827-19534846 MS.gene63549:intron 25.0%
!! GTTTGAGATTTGTAACATAT+TGG + chr1.4:19534705-19534724 None:intergenic 25.0%
!!! AAGTGGAAGAAATTTTTTAG+AGG + chr1.4:19534986-19535005 None:intergenic 25.0%
!!! ATTGGTCCTTTAAGTTATTT+TGG + chr1.4:19534687-19534706 None:intergenic 25.0%
!!! TGAAGATGAAATTTTTTTGG+AGG + chr1.4:19535014-19535033 None:intergenic 25.0%
AATATTACCAAAGGAGGAAA+TGG - chr1.4:19535607-19535626 MS.gene63549:CDS 30.0%
CATATGCAATGTTAAAGAGT+AGG + chr1.4:19535079-19535098 None:intergenic 30.0%
CATTAGATGAAATTGTGACA+TGG + chr1.4:19534360-19534379 None:intergenic 30.0%
CTACTCTTTAACATTGCATA+TGG - chr1.4:19535077-19535096 MS.gene63549:intron 30.0%
GACAATGCATAATATATGCA+AGG + chr1.4:19534180-19534199 None:intergenic 30.0%
GTGTGACCAAAATAACTTAA+AGG - chr1.4:19534678-19534697 MS.gene63549:intron 30.0%
GTGTGACCAAAATAACTTAA+AGG + chr1.4:19534697-19534678 None:intergenic 30.0%
GTTACAAACCTCAAACTTAA+AGG - chr1.4:19534769-19534788 MS.gene63549:intron 30.0%
GTTACAAACCTCAAACTTAA+AGG + chr1.4:19534788-19534769 None:intergenic 30.0%
GTTCAAAATATTACCAAAGG+AGG - chr1.4:19535601-19535620 MS.gene63549:CDS 30.0%
GTTGTGATTGAATTTATTGG+TGG - chr1.4:19535538-19535557 MS.gene63549:CDS 30.0%
TTAGATGAAATTGTGACATG+GGG + chr1.4:19534358-19534377 None:intergenic 30.0%
! ACTGAATGATTGACTCAATT+AGG + chr1.4:19534277-19534296 None:intergenic 30.0%
! ACTTTTTATGCATGGCATAT+TGG - chr1.4:19534040-19534059 MS.gene63549:intron 30.0%
! ATAGATGTCCTTTAAGTTTG+AGG - chr1.4:19534441-19534460 MS.gene63549:intron 30.0%
! ATGAATGTATAGCCTTTTGT+TGG + chr1.4:19535196-19535215 None:intergenic 30.0%
! GTTTGAGGTTTGTAACAAAT+TGG - chr1.4:19534456-19534475 MS.gene63549:intron 30.0%
!!! TTTTTTTGGAGGACTAAAAG+TGG + chr1.4:19535003-19535022 None:intergenic 30.0%
AATGGAACAACATTGCATGT+TGG + chr1.4:19533432-19533451 None:intergenic 35.0%
ACACTAGTTAACTATCGTTC+CGG + chr1.4:19533839-19533858 None:intergenic 35.0%
ATGGAACAACATTGCATGTT+GGG + chr1.4:19533431-19533450 None:intergenic 35.0%
CATAATATATGCAAGGTCTG+GGG + chr1.4:19534173-19534192 None:intergenic 35.0%
GCATAATATATGCAAGGTCT+GGG + chr1.4:19534174-19534193 None:intergenic 35.0%
GTCTTCAAGCTAATGGTATT+AGG - chr1.4:19535464-19535483 MS.gene63549:intron 35.0%
GTTAATGGTCTTCAAGCTAA+TGG - chr1.4:19535457-19535476 MS.gene63549:intron 35.0%
TAAGAGATCCCCATATATTG+TGG - chr1.4:19533952-19533971 MS.gene63549:intron 35.0%
TACACCCTACACAAATAAAG+AGG + chr1.4:19533303-19533322 None:intergenic 35.0%
TAGATGAAATTGTGACATGG+GGG + chr1.4:19534357-19534376 None:intergenic 35.0%
TGCATAATATATGCAAGGTC+TGG + chr1.4:19534175-19534194 None:intergenic 35.0%
TGTGTAGGGTGTAAAGAATA+TGG - chr1.4:19533310-19533329 MS.gene63549:CDS 35.0%
TTATGGAGATAGTTAGTACC+TGG + chr1.4:19533460-19533479 None:intergenic 35.0%
TTCAATTTCTGTGTTGAGCA+TGG + chr1.4:19535257-19535276 None:intergenic 35.0%
! ACACAAGTCCTTTAAGTTTG+AGG - chr1.4:19534501-19534520 MS.gene63549:intron 35.0%
! ACTTCATGACTTTTGTGCTT+TGG - chr1.4:19533372-19533391 MS.gene63549:CDS 35.0%
! CTGACCTCTTTATTTGTGTA+GGG - chr1.4:19533296-19533315 MS.gene63549:CDS 35.0%
! GGAAATGGAAGATTTTATGG+TGG - chr1.4:19534830-19534849 MS.gene63549:intron 35.0%
! GTAACCGATTTAAAAAGTGC+CGG - chr1.4:19533817-19533836 MS.gene63549:intron 35.0%
! TGGCATATTGGCATTTCTTT+TGG - chr1.4:19534052-19534071 MS.gene63549:intron 35.0%
!! GAAAGAACACTTGGTTTTTC+AGG + chr1.4:19535493-19535512 None:intergenic 35.0%
!! GAAGGTATTGGTGAAAGTAT+GGG - chr1.4:19535568-19535587 MS.gene63549:CDS 35.0%
!! TGAAGGTATTGGTGAAAGTA+TGG - chr1.4:19535567-19535586 MS.gene63549:CDS 35.0%
!! TTTATTGGTGGGTTTGTTGA+AGG - chr1.4:19535550-19535569 MS.gene63549:CDS 35.0%
!!! TTTTGTTTTTGTGATGTGGC+CGG - chr1.4:19534139-19534158 MS.gene63549:intron 35.0%
AAATCCAAATGCGACGTTTG+TGG - chr1.4:19535166-19535185 MS.gene63549:intron 40.0%
AACGATCGTGTTGGAAAGAT+CGG + chr1.4:19535347-19535366 None:intergenic 40.0%
AATGGAAAGGATGAGCCTTA+AGG + chr1.4:19533408-19533427 None:intergenic 40.0%
ACAACATTGCATGTTGGGAA+TGG + chr1.4:19533426-19533445 None:intergenic 40.0%
ACAATGATATGGCAGAAGGA+AGG + chr1.4:19534606-19534625 None:intergenic 40.0%
ACGAACAGCTATTGTCTGAT+AGG + chr1.4:19533720-19533739 None:intergenic 40.0%
AGATGAAATTGTGACATGGG+GGG + chr1.4:19534356-19534375 None:intergenic 40.0%
ATGGAAAGGATGAGCCTTAA+GGG + chr1.4:19533407-19533426 None:intergenic 40.0%
ATGTTGTTCCATTCCAAACC+AGG - chr1.4:19533439-19533458 MS.gene63549:CDS 40.0%
ATTGCATGTTGGGAATGGAA+AGG + chr1.4:19533421-19533440 None:intergenic 40.0%
CACAAATAAAGAGGTCAGGA+AGG + chr1.4:19533294-19533313 None:intergenic 40.0%
CCAAACAATGATATGGCAGA+AGG + chr1.4:19534610-19534629 None:intergenic 40.0%
CCTACACAAATAAAGAGGTC+AGG + chr1.4:19533298-19533317 None:intergenic 40.0%
CCTCAAACTTAAAGGACTCT+TGG - chr1.4:19534777-19534796 MS.gene63549:intron 40.0%
CCTTCTGCCATATCATTGTT+TGG - chr1.4:19534607-19534626 MS.gene63549:intron 40.0%
GAGATAGTTAGTACCTGGTT+TGG + chr1.4:19533455-19533474 None:intergenic 40.0%
GTTTGTGGCAAACCAACAAA+AGG - chr1.4:19535181-19535200 MS.gene63549:intron 40.0%
TACCAACTGAGTTAAGCTCA+CGG - chr1.4:19534202-19534221 MS.gene63549:intron 40.0%
TAGAGTTCCATTTCCTCCTT+TGG + chr1.4:19535617-19535636 None:intergenic 40.0%
! CCAAGAGTCCTTTAAGTTTG+AGG + chr1.4:19534780-19534799 None:intergenic 40.0%
! CCTGACCTCTTTATTTGTGT+AGG - chr1.4:19533295-19533314 MS.gene63549:CDS 40.0%
! TGAGAGTGTGTGGATGATTT+GGG + chr1.4:19535282-19535301 None:intergenic 40.0%
! TGAGCTTAACTCAGTTGGTA+GGG + chr1.4:19534202-19534221 None:intergenic 40.0%
! TGTGTAAGTTTGAGAGTGTG+TGG + chr1.4:19535292-19535311 None:intergenic 40.0%
! TTGAGAGTGTGTGGATGATT+TGG + chr1.4:19535283-19535302 None:intergenic 40.0%
! TTTATGGTGGAGCTAAAGAG+TGG - chr1.4:19534843-19534862 MS.gene63549:intron 40.0%
!! AGTTAGTACCTGGTTTGGAA+TGG + chr1.4:19533450-19533469 None:intergenic 40.0%
!! CGTTCCGGCACTTTTTAAAT+CGG + chr1.4:19533824-19533843 None:intergenic 40.0%
!!! TTTGTTTTTGTGATGTGGCC+GGG - chr1.4:19534140-19534159 MS.gene63549:intron 40.0%
ACACATTCAACAACCACCAC+CGG - chr1.4:19535307-19535326 MS.gene63549:intron 45.0%
ACCAACTGAGTTAAGCTCAC+GGG - chr1.4:19534203-19534222 MS.gene63549:intron 45.0%
GGGTGTAAAGAATATGGCTC+TGG - chr1.4:19533316-19533335 MS.gene63549:CDS 45.0%
GTGAGCTTAACTCAGTTGGT+AGG + chr1.4:19534203-19534222 None:intergenic 45.0%
TGGCACAACATGGATGCATT+TGG + chr1.4:19535237-19535256 None:intergenic 45.0%
TTTGCCACAAACGTCGCATT+TGG + chr1.4:19535173-19535192 None:intergenic 45.0%
! GCATTTCTTTTGGCGCATAG+TGG - chr1.4:19534062-19534081 MS.gene63549:intron 45.0%
! GGAAAGATCGGTGTTTGTTG+CGG + chr1.4:19535335-19535354 None:intergenic 45.0%
!! GGTGGGTTTGTTGAAGGTAT+TGG - chr1.4:19535556-19535575 MS.gene63549:CDS 45.0%
!!! TTGTTTTTGTGATGTGGCCG+GGG - chr1.4:19534141-19534160 MS.gene63549:intron 45.0%
AAAGGATGAGCCTTAAGGGC+AGG + chr1.4:19533403-19533422 None:intergenic 50.0%
ACATTCAACAACCACCACCG+GGG - chr1.4:19535309-19535328 MS.gene63549:intron 50.0%
AGTGCAAGAAACGAAGGTCG+GGG - chr1.4:19535374-19535393 MS.gene63549:intron 50.0%
CACATTCAACAACCACCACC+GGG - chr1.4:19535308-19535327 MS.gene63549:intron 50.0%
CCAACTGAGTTAAGCTCACG+GGG - chr1.4:19534204-19534223 MS.gene63549:intron 50.0%
CGAGTGCAAGAAACGAAGGT+CGG - chr1.4:19535372-19535391 MS.gene63549:intron 50.0%
CTCGTTGCAAACGATCGTGT+TGG + chr1.4:19535356-19535375 None:intergenic 50.0%
GAGTGCAAGAAACGAAGGTC+GGG - chr1.4:19535373-19535392 MS.gene63549:intron 50.0%
GCAACGAGTGCAAGAAACGA+AGG - chr1.4:19535368-19535387 MS.gene63549:intron 50.0%
GCAGCAGCAGAAAGAACACT+TGG + chr1.4:19535502-19535521 None:intergenic 50.0%
GTGTTGAGCATGGCACAACA+TGG + chr1.4:19535247-19535266 None:intergenic 50.0%
! GAAGGTCGGGGAAAGTGTAT+CGG - chr1.4:19535386-19535405 MS.gene63549:intron 50.0%
CCCCGTGAGCTTAACTCAGT+TGG + chr1.4:19534207-19534226 None:intergenic 55.0%
! AAGGTCTGGGGTTCAAACCC+CGG + chr1.4:19534161-19534180 None:intergenic 55.0%
! GGGGAAAGTGTATCGGTGCA+CGG - chr1.4:19535393-19535412 MS.gene63549:intron 55.0%
! TTTGGCTCCTCCTGCCCTTA+AGG - chr1.4:19533390-19533409 MS.gene63549:CDS 55.0%
GGATGAGCCTTAAGGGCAGG+AGG + chr1.4:19533400-19533419 None:intergenic 60.0%
! ATCGGTGTTTGTTGCGGCCC+CGG + chr1.4:19535329-19535348 None:intergenic 60.0%
! GGTGTTTGTTGCGGCCCCGG+TGG + chr1.4:19535326-19535345 None:intergenic 70.0%
! GTTTGTTGCGGCCCCGGTGG+TGG + chr1.4:19535323-19535342 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 19533283 19535678 19533283 ID=MS.gene63549
chr1.4 mRNA 19533283 19535678 19533283 ID=MS.gene63549.t1;Parent=MS.gene63549
chr1.4 exon 19535501 19535678 19535501 ID=MS.gene63549.t1.exon1;Parent=MS.gene63549.t1
chr1.4 CDS 19535501 19535678 19535501 ID=cds.MS.gene63549.t1;Parent=MS.gene63549.t1
chr1.4 exon 19533283 19533812 19533283 ID=MS.gene63549.t1.exon2;Parent=MS.gene63549.t1
chr1.4 CDS 19533283 19533812 19533283 ID=cds.MS.gene63549.t1;Parent=MS.gene63549.t1
Gene Sequence

>MS.gene63549

ATGGTGGACCTTCCTGACCTCTTTATTTGTGTAGGGTGTAAAGAATATGGCTCTGGCAAGAGATTTGTTTGTCAACAATGTAATTATCAACTTCATGACTTTTGTGCTTTGGCTCCTCCTGCCCTTAAGGCTCATCCTTTCCATTCCCAACATGCAATGTTGTTCCATTCCAAACCAGGTACTAACTATCTCCATAAATTTTACCAATTAATAGTTGAATTATTTGATTATATGATTTATAACTTTGTTGTTAACAAATTATTATAGTTTAAATATAATGATTTTTTTTCTGTGACAGAATTTTAATATAATGATTAAATGAAGTACTAATTCGTATTTACATTTGAAATTATTACAAGTTGTAGATGTCTATGATACATTTTTTTAATAAAAAAACAAACTCTTTAAGCATCTAGCAATTACTCACAGAGACACCTATCAGACAATAGCTGTTCGTTGTTAAATGATAGTTAACAATTGTTTCATAGTTTCTAAATCGGTAAAATCACCTATTAAATTGGTTCATTTAAATCGGTAACCGATTTAAAAAGTGCCGGAACGATAGTTAACTAGTGTTTTATATGTCTATAAGTAATCGGTAGATCTCTAAAGAGCAGTCTTAAAATTAAACTTGCTCTCTTTTAATAAGTTTGTATTTTTCGATCTTTGTAAGAGATCCCCATATATTGTGGCATTAAATAGGCTTTTGCTTTCCTCAGTTTTATTTTTTGTAAATCCAATATTTTCCATTTTATTTACTTTTTATGCATGGCATATTGGCATTTCTTTTGGCGCATAGTGGCATATTCTTAAACAATGATTAGTTCAAATTCTAATCTTTACTCTAATATTTTTTTTTTGTTTTTGTGATGTGGCCGGGGTTTGAACCCCAGACCTTGCATATATTATGCATTGTCCCTACCAACTGAGTTAAGCTCACGGGGACACAATTATACTAATTTTAACTAATACGGAGCTCTTTAAAACAAAGCCTAATTGAGTCAATCATTCAGTTTGAAAGAATGAATTAAAACAGAAAAAATTGAAAGAATTAAAATATGAGAATATATCCCCCCATGTCACAATTTCATCTAATGAAATCTCCACCATCAATAATAATAAAATATAGATGGATAGTTTAAAATAGACTAAATTACAATAGATGTCCTTTAAGTTTGAGGTTTGTAACAAATTGGTTCTTTAAGTTATTTTGCTCTCACACAAGTCCTTTAAGTTTGAGGTTTGTAACAGATTAATGATCCTTTAAGTTATTTTGGTCACACATAAGTCCTTTAAGTTTGTAAACATTTTCGATTTAGTCCTTCCTTCTGCCATATCATTGTTTGGTTAACTTAAGGACTAAATTGAAAACGTTTACAAACTTAAAGGACTTATGTGTGACCAAAATAACTTAAAGGACCAATATGTTACAAATCTCAAACTTAAAGGACTTATGTGTGATCAAAATAACTTAAATGACTAATCTGTTACAAACCTCAAACTTAAAGGACTCTTGGTGTAATTTAGCCTTTAAAATATAATAAATAGGAAATGGAAGATTTTATGGTGGAGCTAAAGAGTGGTGAAGCTTGAACTAACTTTTTTAGCGAATCAAAATAAATATGGCTTAATTGCAGTTTTAAAAATAAATGTTAAATATATTTTGATCCCTATAAATATTTCAACTTTTCATTTTAGTTCCTCTAAAAAATTTCTTCCACTTTTAGTCCTCCAAAAAAATTTCATCTTCACTTTTGGTCCCTCATTTAAAGTAAACTCAAATTGTGTACATCCCTACTCTTTAACATTGCATATGGTTCTTGATTTCATTCTCATTATGGCGTGCATGAATTTTATATATTTCAGTGAAAAGTGGAAAGACAAAATCCAAATGCGACGTTTGTGGCAAACCAACAAAAGGCTATACATTCATATGCACTGCATGCGCTTTCCAAATGCATCCATGTTGTGCCATGCTCAACACAGAAATTGAATTCCCAAATCATCCACACACTCTCAAACTTACACATTCAACAACCACCACCGGGGCCGCAACAAACACCGATCTTTCCAACACGATCGTTTGCAACGAGTGCAAGAAACGAAGGTCGGGGAAAGTGTATCGGTGCACGGTGTGTAACTACAATCTTCATGCATTTTGTGCTAAGAATAAAGTTAATGGTCTTCAAGCTAATGGTATTAGGCTTCCTGAAAAACCAAGTGTTCTTTCTGCTGCTGCTAAAGTTGCTTCTCAAGTTGTGATTGAATTTATTGGTGGGTTTGTTGAAGGTATTGGTGAAAGTATGGGTGAAGTTCTTGTTCAAAATATTACCAAAGGAGGAAATGGAACTCTAACCAGTGCTAATAATAATACTACTACAAAACCTCGTAAATAA
Protein sequence

>MS.gene63549.t1

MVDLPDLFICVGCKEYGSGKRFVCQQCNYQLHDFCALAPPALKAHPFHSQHAMLFHSKPVKSGKTKSKCDVCGKPTKGYTFICTACAFQMHPCCAMLNTEIEFPNHPHTLKLTHSTTTTGAATNTDLSNTIVCNECKKRRSGKVYRCTVCNYNLHAFCAKNKVNGLQANGIRLPEKPSVLSAAAKVASQVVIEFIGGFVEGIGESMGEVLVQNITKGGNGTLTSANNNTTTKPRK