Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63551.t1 | XP_013468026.1 | 98.4 | 62 | 1 | 0 | 1 | 62 | 109 | 170 | 3.40E-27 | 130.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63551.t1 | A0A072VKV4 | 98.4 | 62 | 1 | 0 | 1 | 62 | 109 | 170 | 2.4e-27 | 130.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene63551 | 0.800562 | 1.36E-48 | -1.69E-46 |
| MS.gene052609 | MS.gene63551 | 0.811419 | 7.07E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene63551 | 0.803193 | 3.92E-49 | -1.69E-46 |
| MS.gene059439 | MS.gene63551 | 0.804057 | 2.59E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATCCATCTAAAAGCATT+AGG | 0.316447 | 3.4:-7219334 | MS.gene63551:CDS |
| ATACTTGGATTCTTGAGGTT+TGG | 0.380840 | 3.4:+7219031 | None:intergenic |
| TGCAAAAGGAGGAGCAACTT+GGG | 0.454385 | 3.4:-7219058 | MS.gene63551:CDS |
| TTCATCTGCCACTGTTCCTA+AGG | 0.455578 | 3.4:-7219237 | MS.gene63551:intron |
| ATGCAAAAGGAGGAGCAACT+TGG | 0.467583 | 3.4:-7219059 | MS.gene63551:CDS |
| AAACCATACTTGGATTCTTG+AGG | 0.544459 | 3.4:+7219026 | None:intergenic |
| TTAAATGTGCAGAGAATGGA+AGG | 0.560081 | 3.4:-7219363 | None:intergenic |
| TCAGCATAATATGCAAAAGG+AGG | 0.605517 | 3.4:-7219069 | MS.gene63551:intron |
| GCAAAAGGAGGAGCAACTTG+GGG | 0.648508 | 3.4:-7219057 | MS.gene63551:CDS |
| GACTTGTACCTTAGGAACAG+TGG | 0.679559 | 3.4:+7219229 | None:intergenic |
| ACAGTGGCAGATGAAAACTG+AGG | 0.800494 | 3.4:+7219245 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAATTAGAGTAAAATAT+AGG | + | chr3.4:7219185-7219204 | None:intergenic | 10.0% |
| !!! | TAAGTATATATTTTGCTTTT+GGG | - | chr3.4:7219234-7219253 | MS.gene63551:intron | 15.0% |
| !!! | TTAAGTATATATTTTGCTTT+TGG | - | chr3.4:7219233-7219252 | MS.gene63551:intron | 15.0% |
| !! | AAATTAGAGTAAAATATAGG+AGG | + | chr3.4:7219182-7219201 | None:intergenic | 20.0% |
| ! | ATATACTATAACTTCATTCC+TGG | + | chr3.4:7219214-7219233 | None:intergenic | 25.0% |
| !! | TTTTCAGCATAATATGCAAA+AGG | - | chr3.4:7219314-7219333 | MS.gene63551:CDS | 25.0% |
| !!! | AAAAGCATTAGGTTTTCTTA+CGG | - | chr3.4:7219063-7219082 | MS.gene63551:CDS | 25.0% |
| !!! | AATATCCATCTAAAAGCATT+AGG | - | chr3.4:7219052-7219071 | MS.gene63551:CDS | 25.0% |
| CTCTAATTTAATTGTTTGCC+AGG | - | chr3.4:7219193-7219212 | MS.gene63551:intron | 30.0% | |
| ! | ATTAGGTTTTCTTACGGAAT+TGG | - | chr3.4:7219069-7219088 | MS.gene63551:intron | 30.0% |
| ! | GAAAACCTAATGCTTTTAGA+TGG | + | chr3.4:7219060-7219079 | None:intergenic | 30.0% |
| ! | TTAGGTTTTCTTACGGAATT+GGG | - | chr3.4:7219070-7219089 | MS.gene63551:intron | 30.0% |
| TCAGCATAATATGCAAAAGG+AGG | - | chr3.4:7219317-7219336 | MS.gene63551:CDS | 35.0% | |
| TATAGGAGGACTTGTACCTT+AGG | + | chr3.4:7219168-7219187 | None:intergenic | 40.0% | |
| ACAGTGGCAGATGAAAACTG+AGG | + | chr3.4:7219144-7219163 | None:intergenic | 45.0% | |
| ATGCAAAAGGAGGAGCAACT+TGG | - | chr3.4:7219327-7219346 | MS.gene63551:CDS | 45.0% | |
| GACTTGTACCTTAGGAACAG+TGG | + | chr3.4:7219160-7219179 | None:intergenic | 45.0% | |
| TGCAAAAGGAGGAGCAACTT+GGG | - | chr3.4:7219328-7219347 | MS.gene63551:CDS | 45.0% | |
| TTCATCTGCCACTGTTCCTA+AGG | - | chr3.4:7219149-7219168 | MS.gene63551:intron | 45.0% | |
| GCAAAAGGAGGAGCAACTTG+GGG | - | chr3.4:7219329-7219348 | MS.gene63551:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 7219035 | 7219373 | 7219035 | ID=MS.gene63551 |
| chr3.4 | mRNA | 7219035 | 7219373 | 7219035 | ID=MS.gene63551.t1;Parent=MS.gene63551 |
| chr3.4 | exon | 7219238 | 7219373 | 7219238 | ID=MS.gene63551.t1.exon1;Parent=MS.gene63551.t1 |
| chr3.4 | CDS | 7219238 | 7219373 | 7219238 | ID=cds.MS.gene63551.t1;Parent=MS.gene63551.t1 |
| chr3.4 | exon | 7219035 | 7219087 | 7219035 | ID=MS.gene63551.t1.exon2;Parent=MS.gene63551.t1 |
| chr3.4 | CDS | 7219035 | 7219087 | 7219035 | ID=cds.MS.gene63551.t1;Parent=MS.gene63551.t1 |
| Gene Sequence |
| Protein sequence |
