Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64005.t1 | AES59710.2 | 97 | 304 | 8 | 1 | 1 | 304 | 48 | 350 | 1.90E-163 | 585.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64005.t1 | G7I747 | 97.0 | 304 | 8 | 1 | 1 | 304 | 48 | 350 | 1.3e-163 | 585.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050227 | MS.gene64005 | 0.801247 | 9.85E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene64005 | 0.806636 | 7.47E-50 | -1.69E-46 |
| MS.gene051210 | MS.gene64005 | 0.813045 | 3.12E-51 | -1.69E-46 |
| MS.gene051705 | MS.gene64005 | 0.80242 | 5.66E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene64005 | 0.805803 | 1.12E-49 | -1.69E-46 |
| MS.gene059725 | MS.gene64005 | 0.810554 | 1.09E-50 | -1.69E-46 |
| MS.gene060637 | MS.gene64005 | 0.813487 | 2.50E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64005.t1 | MTR_1g024830 | 97.039 | 304 | 8 | 1 | 1 | 304 | 48 | 350 | 0.0 | 611 |
| MS.gene64005.t1 | MTR_5g022570 | 38.667 | 300 | 159 | 9 | 12 | 304 | 17 | 298 | 1.03e-54 | 180 |
| MS.gene64005.t1 | MTR_1g077100 | 44.545 | 110 | 55 | 3 | 195 | 299 | 17 | 125 | 2.22e-22 | 90.9 |
| MS.gene64005.t1 | MTR_8g085310 | 24.828 | 290 | 188 | 9 | 18 | 294 | 434 | 706 | 3.44e-13 | 70.5 |
| MS.gene64005.t1 | MTR_1g055365 | 28.261 | 138 | 94 | 3 | 12 | 145 | 19 | 155 | 1.19e-12 | 68.6 |
| MS.gene64005.t1 | MTR_4g092300 | 23.208 | 293 | 189 | 9 | 16 | 294 | 554 | 824 | 3.66e-11 | 64.3 |
| MS.gene64005.t1 | MTR_4g051480 | 23.322 | 283 | 182 | 8 | 16 | 284 | 572 | 833 | 3.69e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64005.t1 | AT5G05800 | 26.000 | 300 | 190 | 9 | 5 | 295 | 160 | 436 | 9.18e-17 | 80.5 |
| MS.gene64005.t1 | AT5G05800 | 26.000 | 300 | 190 | 9 | 5 | 295 | 160 | 436 | 9.18e-17 | 80.5 |
| MS.gene64005.t1 | AT5G05800 | 26.000 | 300 | 190 | 9 | 5 | 295 | 160 | 436 | 9.18e-17 | 80.5 |
| MS.gene64005.t1 | AT5G05800 | 26.000 | 300 | 190 | 9 | 5 | 295 | 160 | 436 | 9.18e-17 | 80.5 |
| MS.gene64005.t1 | AT5G05800 | 26.000 | 300 | 190 | 9 | 5 | 295 | 160 | 436 | 9.18e-17 | 80.5 |
| MS.gene64005.t1 | AT3G11290 | 24.516 | 310 | 182 | 10 | 13 | 294 | 163 | 448 | 2.63e-15 | 76.3 |
| MS.gene64005.t1 | AT3G11290 | 24.516 | 310 | 182 | 10 | 13 | 294 | 163 | 448 | 2.63e-15 | 76.3 |
| MS.gene64005.t1 | AT2G24960 | 31.288 | 163 | 95 | 6 | 12 | 164 | 9 | 164 | 2.00e-14 | 74.3 |
| MS.gene64005.t1 | AT2G24960 | 31.288 | 163 | 95 | 6 | 12 | 164 | 9 | 164 | 2.34e-14 | 73.9 |
| MS.gene64005.t1 | AT2G24960 | 31.293 | 147 | 96 | 3 | 2 | 144 | 311 | 456 | 2.90e-12 | 67.8 |
| MS.gene64005.t1 | AT4G02210 | 24.242 | 297 | 169 | 8 | 13 | 297 | 181 | 433 | 2.84e-12 | 67.4 |
| MS.gene64005.t1 | AT4G02210 | 24.242 | 297 | 169 | 8 | 13 | 297 | 181 | 433 | 2.84e-12 | 67.4 |
| MS.gene64005.t1 | AT2G19220 | 24.834 | 302 | 178 | 12 | 13 | 294 | 160 | 432 | 5.12e-12 | 66.6 |
Find 61 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGACTTCATATCCAGAAAT+TGG | 0.154307 | 1.4:-816422 | MS.gene64005:CDS |
| TAATGTGAGACCCCAATTTC+TGG | 0.181085 | 1.4:+816410 | None:intergenic |
| CTCTTGCTTGGGTTGATTAC+TGG | 0.204104 | 1.4:+817013 | None:intergenic |
| ATTGATTGTTATTGAAATTA+AGG | 0.208751 | 1.4:+816844 | None:intergenic |
| TTTCCTATCAAGAGAATTCT+TGG | 0.233247 | 1.4:+816328 | None:intergenic |
| TGACAAGATATTTGCAGATT+TGG | 0.250594 | 1.4:-816961 | MS.gene64005:CDS |
| ACATTCGTGATGAATTTAAT+AGG | 0.289952 | 1.4:-816875 | MS.gene64005:CDS |
| CCTTTAACTGTTTCATTCCT+AGG | 0.298916 | 1.4:+816569 | None:intergenic |
| CCTAGGAATGAAACAGTTAA+AGG | 0.309684 | 1.4:-816569 | MS.gene64005:CDS |
| TGTCCTTGAACGCTCTTGCT+TGG | 0.338161 | 1.4:+817001 | None:intergenic |
| AGCCTTGATGCAGTTTGTTA+TGG | 0.339766 | 1.4:+816169 | None:intergenic |
| TGACTTCATATCCAGAAATT+GGG | 0.364560 | 1.4:-816421 | MS.gene64005:CDS |
| CTAGTAGTTTGTGTCTCATT+AGG | 0.366997 | 1.4:+816296 | None:intergenic |
| GTCCTTGAACGCTCTTGCTT+GGG | 0.382970 | 1.4:+817002 | None:intergenic |
| CTATGTCTTCCCACTGTTCA+AGG | 0.395402 | 1.4:+816721 | None:intergenic |
| CTGTTCAAGGCCAATGTAAA+GGG | 0.400976 | 1.4:+816734 | None:intergenic |
| TGACCAAGAATTCTCTTGAT+AGG | 0.403511 | 1.4:-816331 | MS.gene64005:CDS |
| GGTTGTTAAGCAGATTCAAC+TGG | 0.405063 | 1.4:-816940 | MS.gene64005:CDS |
| AACCCAAGCAAGAGCGTTCA+AGG | 0.406926 | 1.4:-817004 | MS.gene64005:CDS |
| TCAACTGTTGAGATATTCCT+TGG | 0.417577 | 1.4:+816359 | None:intergenic |
| TATGCTCAATCTAGTCACTA+TGG | 0.423572 | 1.4:-816482 | MS.gene64005:CDS |
| AACAAACTGCATCAAGGCTC+TGG | 0.436556 | 1.4:-816165 | MS.gene64005:CDS |
| GCACAAAGTTGCTCATATAT+CGG | 0.439076 | 1.4:+816536 | None:intergenic |
| AGAATTCTCTTGATAGGAAA+AGG | 0.441346 | 1.4:-816325 | MS.gene64005:CDS |
| TCAAATTATAGAAGCGCGTC+CGG | 0.441434 | 1.4:+816799 | None:intergenic |
| TGTTGAGATATTCCTTGGAA+TGG | 0.460006 | 1.4:+816364 | None:intergenic |
| AGCCATGTTAGAGATGATTT+CGG | 0.465178 | 1.4:-816240 | MS.gene64005:CDS |
| CTAGGAATGAAACAGTTAAA+GGG | 0.465670 | 1.4:-816568 | MS.gene64005:CDS |
| TTCACAGACTTGCCTGCTGA+TGG | 0.487163 | 1.4:-816509 | MS.gene64005:CDS |
| TGAGCATATTTCCCATCAGC+AGG | 0.494871 | 1.4:+816497 | None:intergenic |
| ATGAATGTCTCTCTGAAACT+AGG | 0.501637 | 1.4:+816083 | None:intergenic |
| TTGAACAGTGGGAAGACATA+GGG | 0.503257 | 1.4:-816719 | MS.gene64005:CDS |
| TTCCATAACAAACTGCATCA+AGG | 0.509166 | 1.4:-816171 | MS.gene64005:CDS |
| ACTGTTCAAGGCCAATGTAA+AGG | 0.509773 | 1.4:+816733 | None:intergenic |
| CTTGAACAGTGGGAAGACAT+AGG | 0.517278 | 1.4:-816720 | MS.gene64005:CDS |
| TTGCAAGGAAAGTGCAGTAA+AGG | 0.520864 | 1.4:-816026 | MS.gene64005:CDS |
| TTTCTATACAGGCGCAGCCT+AGG | 0.523790 | 1.4:-816586 | MS.gene64005:intron |
| GGAAGCATCTTGATGTCCTC+CGG | 0.529344 | 1.4:-816818 | MS.gene64005:CDS |
| TTTACATTGGCCTTGAACAG+TGG | 0.533625 | 1.4:-816731 | MS.gene64005:CDS |
| CATCATCAAAATCCATTCCA+AGG | 0.533699 | 1.4:-816376 | MS.gene64005:CDS |
| TCTACTAATGATCAAAGTAA+TGG | 0.535019 | 1.4:-816774 | MS.gene64005:CDS |
| ATTTCAATAACAATCAATTG+AGG | 0.543496 | 1.4:-816839 | MS.gene64005:CDS |
| TCAAGACACGTGCAATGCTA+TGG | 0.547142 | 1.4:-816267 | MS.gene64005:CDS |
| TCACAGACTTGCCTGCTGAT+GGG | 0.556906 | 1.4:-816508 | MS.gene64005:CDS |
| TTACATTGGCCTTGAACAGT+GGG | 0.559587 | 1.4:-816730 | MS.gene64005:CDS |
| ATAGATGATCCCCTTTACAT+TGG | 0.571269 | 1.4:-816744 | MS.gene64005:CDS |
| GTTGTTAAGCAGATTCAACT+GGG | 0.580999 | 1.4:-816939 | MS.gene64005:CDS |
| ATAAGGTTGACATGGTTGCA+AGG | 0.586136 | 1.4:-816041 | MS.gene64005:CDS |
| GACTTCATATCCAGAAATTG+GGG | 0.588130 | 1.4:-816420 | MS.gene64005:CDS |
| CACCGAAATCATCTCTAACA+TGG | 0.588731 | 1.4:+816238 | None:intergenic |
| TAGAGATGATTTCGGTGTCG+AGG | 0.596844 | 1.4:-816232 | MS.gene64005:CDS |
| TGAAACTAGGATTCTCAAAG+AGG | 0.607185 | 1.4:+816096 | None:intergenic |
| GTGTCAAGATAAGGTTGACA+TGG | 0.607819 | 1.4:-816049 | MS.gene64005:CDS |
| AATTATAGAAGCGCGTCCGG+AGG | 0.609851 | 1.4:+816802 | None:intergenic |
| AAGAGCGTTCAAGGACAAGA+TGG | 0.625198 | 1.4:-816995 | MS.gene64005:CDS |
| CTTAAACCTATACTAACGGA+AGG | 0.630056 | 1.4:+816001 | None:intergenic |
| AAGGCTCTGGATGAAATACA+AGG | 0.630636 | 1.4:-816152 | MS.gene64005:CDS |
| TGAACAGTGGGAAGACATAG+GGG | 0.639240 | 1.4:-816718 | MS.gene64005:intron |
| TGTTCAAGGCCAATGTAAAG+GGG | 0.663197 | 1.4:+816735 | None:intergenic |
| GGAGAGTTAGATAAATCTGT+CGG | 0.664480 | 1.4:-816461 | MS.gene64005:CDS |
| TTGTTAAGCAGATTCAACTG+GGG | 0.729455 | 1.4:-816938 | MS.gene64005:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGATTGTTATTGAAATTA+AGG | + | chr1.4:816198-816217 | None:intergenic | 15.0% |
| !! | AATAATGCATAAGGAAATAT+TGG | + | chr1.4:816370-816389 | None:intergenic | 20.0% |
| !! | AATTTAGAGAATAATGCATA+AGG | + | chr1.4:816379-816398 | None:intergenic | 20.0% |
| !! | ATTTCAATAACAATCAATTG+AGG | - | chr1.4:816200-816219 | MS.gene64005:CDS | 20.0% |
| ! | AATTAGATTTGACTCTTCTA+AGG | + | chr1.4:816412-816431 | None:intergenic | 25.0% |
| ! | ACATTCGTGATGAATTTAAT+AGG | - | chr1.4:816164-816183 | MS.gene64005:CDS | 25.0% |
| ! | GGAAATATTGGTATAAAGAA+AGG | + | chr1.4:816358-816377 | None:intergenic | 25.0% |
| ! | TCTACTAATGATCAAAGTAA+TGG | - | chr1.4:816265-816284 | MS.gene64005:CDS | 25.0% |
| !!! | CTTTAAAAAGTGTCAAGATA+AGG | - | chr1.4:816981-817000 | MS.gene64005:CDS | 25.0% |
| AGAATTCTCTTGATAGGAAA+AGG | - | chr1.4:816714-816733 | MS.gene64005:intron | 30.0% | |
| CTAGGAATGAAACAGTTAAA+GGG | - | chr1.4:816471-816490 | MS.gene64005:CDS | 30.0% | |
| TGACAAGATATTTGCAGATT+TGG | - | chr1.4:816078-816097 | MS.gene64005:CDS | 30.0% | |
| TGACTTCATATCCAGAAATT+GGG | - | chr1.4:816618-816637 | MS.gene64005:intron | 30.0% | |
| TTGACTTCATATCCAGAAAT+TGG | - | chr1.4:816617-816636 | MS.gene64005:intron | 30.0% | |
| TTTCCTATCAAGAGAATTCT+TGG | + | chr1.4:816714-816733 | None:intergenic | 30.0% | |
| ! | ATACTTTTGACAAGAAAACG+TGG | - | chr1.4:816137-816156 | MS.gene64005:CDS | 30.0% |
| ! | ATCTGTTACGTTTTCTATAC+AGG | - | chr1.4:816442-816461 | MS.gene64005:CDS | 30.0% |
| !! | AAAGCTTTACTTTTCTGCAT+TGG | - | chr1.4:816919-816938 | MS.gene64005:CDS | 30.0% |
| AGCCATGTTAGAGATGATTT+CGG | - | chr1.4:816799-816818 | MS.gene64005:CDS | 35.0% | |
| ATAGATGATCCCCTTTACAT+TGG | - | chr1.4:816295-816314 | MS.gene64005:CDS | 35.0% | |
| CATCATCAAAATCCATTCCA+AGG | - | chr1.4:816663-816682 | MS.gene64005:intron | 35.0% | |
| CCTAGGAATGAAACAGTTAA+AGG | - | chr1.4:816470-816489 | MS.gene64005:CDS | 35.0% | |
| CCTTTAACTGTTTCATTCCT+AGG | + | chr1.4:816473-816492 | None:intergenic | 35.0% | |
| GACTTCATATCCAGAAATTG+GGG | - | chr1.4:816619-816638 | MS.gene64005:intron | 35.0% | |
| GCACAAAGTTGCTCATATAT+CGG | + | chr1.4:816506-816525 | None:intergenic | 35.0% | |
| GGAGAGTTAGATAAATCTGT+CGG | - | chr1.4:816578-816597 | MS.gene64005:CDS | 35.0% | |
| GTTGTTAAGCAGATTCAACT+GGG | - | chr1.4:816100-816119 | MS.gene64005:CDS | 35.0% | |
| TATGCTCAATCTAGTCACTA+TGG | - | chr1.4:816557-816576 | MS.gene64005:CDS | 35.0% | |
| TCAACTGTTGAGATATTCCT+TGG | + | chr1.4:816683-816702 | None:intergenic | 35.0% | |
| TGAAACTAGGATTCTCAAAG+AGG | + | chr1.4:816946-816965 | None:intergenic | 35.0% | |
| TGACCAAGAATTCTCTTGAT+AGG | - | chr1.4:816708-816727 | MS.gene64005:intron | 35.0% | |
| TGTTGAGATATTCCTTGGAA+TGG | + | chr1.4:816678-816697 | None:intergenic | 35.0% | |
| TTCCATAACAAACTGCATCA+AGG | - | chr1.4:816868-816887 | MS.gene64005:CDS | 35.0% | |
| TTGTTAAGCAGATTCAACTG+GGG | - | chr1.4:816101-816120 | MS.gene64005:CDS | 35.0% | |
| ! | CTAGTAGTTTGTGTCTCATT+AGG | + | chr1.4:816746-816765 | None:intergenic | 35.0% |
| !! | ATGAATGTCTCTCTGAAACT+AGG | + | chr1.4:816959-816978 | None:intergenic | 35.0% |
| !!! | GGAATGGATTTTGATGATGA+TGG | + | chr1.4:816662-816681 | None:intergenic | 35.0% |
| ACTGTTCAAGGCCAATGTAA+AGG | + | chr1.4:816309-816328 | None:intergenic | 40.0% | |
| ATAAGGTTGACATGGTTGCA+AGG | - | chr1.4:816998-817017 | MS.gene64005:CDS | 40.0% | |
| CACCGAAATCATCTCTAACA+TGG | + | chr1.4:816804-816823 | None:intergenic | 40.0% | |
| CTGTTCAAGGCCAATGTAAA+GGG | + | chr1.4:816308-816327 | None:intergenic | 40.0% | |
| GGTTGTTAAGCAGATTCAAC+TGG | - | chr1.4:816099-816118 | MS.gene64005:CDS | 40.0% | |
| GTGTCAAGATAAGGTTGACA+TGG | - | chr1.4:816990-817009 | MS.gene64005:CDS | 40.0% | |
| TAATGTGAGACCCCAATTTC+TGG | + | chr1.4:816632-816651 | None:intergenic | 40.0% | |
| TGTTCAAGGCCAATGTAAAG+GGG | + | chr1.4:816307-816326 | None:intergenic | 40.0% | |
| TTGAACAGTGGGAAGACATA+GGG | - | chr1.4:816320-816339 | MS.gene64005:CDS | 40.0% | |
| ! | TCAAATTATAGAAGCGCGTC+CGG | + | chr1.4:816243-816262 | None:intergenic | 40.0% |
| ! | TTGCAAGGAAAGTGCAGTAA+AGG | - | chr1.4:817013-817032 | MS.gene64005:CDS | 40.0% |
| !! | AAGGCTCTGGATGAAATACA+AGG | - | chr1.4:816887-816906 | MS.gene64005:CDS | 40.0% |
| !! | AGCCTTGATGCAGTTTGTTA+TGG | + | chr1.4:816873-816892 | None:intergenic | 40.0% |
| !! | TTACATTGGCCTTGAACAGT+GGG | - | chr1.4:816309-816328 | MS.gene64005:CDS | 40.0% |
| !! | TTTACATTGGCCTTGAACAG+TGG | - | chr1.4:816308-816327 | MS.gene64005:CDS | 40.0% |
| AACAAACTGCATCAAGGCTC+TGG | - | chr1.4:816874-816893 | MS.gene64005:CDS | 45.0% | |
| CTATGTCTTCCCACTGTTCA+AGG | + | chr1.4:816321-816340 | None:intergenic | 45.0% | |
| CTTGAACAGTGGGAAGACAT+AGG | - | chr1.4:816319-816338 | MS.gene64005:CDS | 45.0% | |
| TCAAGACACGTGCAATGCTA+TGG | - | chr1.4:816772-816791 | MS.gene64005:CDS | 45.0% | |
| TGAACAGTGGGAAGACATAG+GGG | - | chr1.4:816321-816340 | MS.gene64005:CDS | 45.0% | |
| TGAGCATATTTCCCATCAGC+AGG | + | chr1.4:816545-816564 | None:intergenic | 45.0% | |
| !! | AAGAGCGTTCAAGGACAAGA+TGG | - | chr1.4:816044-816063 | MS.gene64005:CDS | 45.0% |
| !! | CTCTTGCTTGGGTTGATTAC+TGG | + | chr1.4:816029-816048 | None:intergenic | 45.0% |
| !! | TAGAGATGATTTCGGTGTCG+AGG | - | chr1.4:816807-816826 | MS.gene64005:CDS | 45.0% |
| GTCCTTGAACGCTCTTGCTT+GGG | + | chr1.4:816040-816059 | None:intergenic | 50.0% | |
| TCACAGACTTGCCTGCTGAT+GGG | - | chr1.4:816531-816550 | MS.gene64005:CDS | 50.0% | |
| TGTCCTTGAACGCTCTTGCT+TGG | + | chr1.4:816041-816060 | None:intergenic | 50.0% | |
| TTCACAGACTTGCCTGCTGA+TGG | - | chr1.4:816530-816549 | MS.gene64005:CDS | 50.0% | |
| TTTCTATACAGGCGCAGCCT+AGG | - | chr1.4:816453-816472 | MS.gene64005:CDS | 50.0% | |
| ! | AATTATAGAAGCGCGTCCGG+AGG | + | chr1.4:816240-816259 | None:intergenic | 50.0% |
| !! | AACCCAAGCAAGAGCGTTCA+AGG | - | chr1.4:816035-816054 | MS.gene64005:CDS | 50.0% |
| !! | GGAAGCATCTTGATGTCCTC+CGG | - | chr1.4:816221-816240 | MS.gene64005:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 816013 | 817048 | 816013 | ID=MS.gene64005 |
| chr1.4 | mRNA | 816013 | 817048 | 816013 | ID=MS.gene64005.t1;Parent=MS.gene64005 |
| chr1.4 | exon | 816719 | 817048 | 816719 | ID=MS.gene64005.t1.exon1;Parent=MS.gene64005.t1 |
| chr1.4 | CDS | 816719 | 817048 | 816719 | ID=cds.MS.gene64005.t1;Parent=MS.gene64005.t1 |
| chr1.4 | exon | 816013 | 816597 | 816013 | ID=MS.gene64005.t1.exon2;Parent=MS.gene64005.t1 |
| chr1.4 | CDS | 816013 | 816597 | 816013 | ID=cds.MS.gene64005.t1;Parent=MS.gene64005.t1 |
| Gene Sequence |
| Protein sequence |
