Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64018.t1 | XP_013689578.1 | 45.8 | 321 | 155 | 5 | 5 | 313 | 6 | 319 | 2.50E-70 | 275.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64018.t1 | A0A2N9I863 | 43.1 | 346 | 159 | 9 | 1 | 316 | 1 | 338 | 1.8e-65 | 259.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060253 | MS.gene64018 | 0.827901 | 1.21E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64018.t1 | MTR_7g062720 | 40.541 | 148 | 80 | 3 | 1 | 146 | 1 | 142 | 8.28e-32 | 117 |
| MS.gene64018.t1 | MTR_4g037355 | 43.220 | 118 | 56 | 4 | 155 | 263 | 1 | 116 | 7.23e-22 | 90.1 |
| MS.gene64018.t1 | MTR_5g061150 | 33.333 | 111 | 74 | 0 | 40 | 150 | 26 | 136 | 3.51e-18 | 80.1 |
| MS.gene64018.t1 | MTR_4g112730 | 57.971 | 69 | 28 | 1 | 143 | 211 | 2 | 69 | 1.75e-17 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 61 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATCAATGGAACAAATTA+AGG | 0.196151 | 1.4:+658569 | MS.gene64018:CDS |
| AGTCTTATTGCCCTCATTAA+TGG | 0.201947 | 1.4:+658549 | MS.gene64018:CDS |
| AAAGTTGTGGCGTTTATTTC+AGG | 0.203868 | 1.4:-658865 | None:intergenic |
| GACAAAATTAAGGCAAGATT+TGG | 0.257188 | 1.4:+658514 | MS.gene64018:CDS |
| GATTCTTGAGGGAGACGATT+TGG | 0.271296 | 1.4:+658372 | MS.gene64018:CDS |
| CTCAATTAAGTTGTTGTTCC+AGG | 0.274617 | 1.4:-658323 | None:intergenic |
| CATAGGAGAAGATATTAATA+TGG | 0.311554 | 1.4:+659275 | MS.gene64018:CDS |
| ATTTACTGAATACTCTGATT+TGG | 0.313227 | 1.4:+658759 | MS.gene64018:CDS |
| CTTGATGATTCTTGTTGGTT+TGG | 0.339105 | 1.4:-659041 | None:intergenic |
| GAAGAGGTTATATGAATTAT+AGG | 0.339571 | 1.4:+659004 | MS.gene64018:intron |
| GGGAGATGTCCCAGAGGAAT+TGG | 0.345733 | 1.4:+658393 | MS.gene64018:CDS |
| ATTCTTGAGGGAGACGATTT+GGG | 0.354692 | 1.4:+658373 | MS.gene64018:CDS |
| AAGACAGGTAGTATCATTCT+TGG | 0.359896 | 1.4:-659238 | None:intergenic |
| TTTACCTTGATGATTCTTGT+TGG | 0.362649 | 1.4:-659046 | None:intergenic |
| CTTGAGGCTGCTGATTTCAT+AGG | 0.363536 | 1.4:+659258 | MS.gene64018:CDS |
| GATTTCTAGAGGGACCTTCC+TGG | 0.373636 | 1.4:-659080 | None:intergenic |
| CATAGCCACCACGTCCTCTT+TGG | 0.374275 | 1.4:-658915 | None:intergenic |
| ACAAAATTAAGGCAAGATTT+GGG | 0.416566 | 1.4:+658515 | MS.gene64018:CDS |
| TATAACTGAAAAGGAAAAGT+TGG | 0.422757 | 1.4:+658672 | MS.gene64018:CDS |
| TGCCCTCATTAATGGATCAA+TGG | 0.432677 | 1.4:+658557 | MS.gene64018:CDS |
| AGATACAAAGAATCTGTTGA+TGG | 0.446469 | 1.4:+659180 | MS.gene64018:CDS |
| TGGCAAAACTATAACTGAAA+AGG | 0.467052 | 1.4:+658663 | MS.gene64018:CDS |
| ATCTGTTGATGGAAAGGAAA+AGG | 0.477668 | 1.4:+659191 | MS.gene64018:CDS |
| AACCACCAGTTGCGTCCTAC+AGG | 0.478404 | 1.4:+658829 | MS.gene64018:CDS |
| TTGTTCCTGTAGGACGCAAC+TGG | 0.480977 | 1.4:-658834 | None:intergenic |
| AGACAGGTAGTATCATTCTT+GGG | 0.483328 | 1.4:-659237 | None:intergenic |
| ATCAAGCACCACATTGATGA+TGG | 0.492315 | 1.4:+658445 | MS.gene64018:CDS |
| TGCAACAACAATATAGAATG+AGG | 0.496917 | 1.4:+658734 | MS.gene64018:CDS |
| CTGGAGACAACTACATAACC+TGG | 0.502040 | 1.4:+658305 | MS.gene64018:CDS |
| AACAAGAATCATCAAGGTAA+AGG | 0.504207 | 1.4:+659048 | MS.gene64018:CDS |
| GTTCCATTGATCCATTAATG+AGG | 0.509644 | 1.4:-658560 | None:intergenic |
| CAAAGAATCTGTTGATGGAA+AGG | 0.510693 | 1.4:+659185 | MS.gene64018:CDS |
| TGTTATAGATGCGGAAAGAA+AGG | 0.513070 | 1.4:+659114 | MS.gene64018:CDS |
| TTCCATTGATCCATTAATGA+GGG | 0.533283 | 1.4:-658559 | None:intergenic |
| GATGCGGAAAGAAAGGTCAT+TGG | 0.533423 | 1.4:+659121 | MS.gene64018:CDS |
| TTTGGTTACAACTCTTCTAG+TGG | 0.535203 | 1.4:+658777 | MS.gene64018:CDS |
| GGACTCGACAAGATTCTTGA+GGG | 0.539594 | 1.4:+658361 | MS.gene64018:CDS |
| TTGGTTTGGTCATATTTGAG+AGG | 0.544297 | 1.4:-659027 | None:intergenic |
| GAAATCAGCAGCCTCAAGAC+AGG | 0.558951 | 1.4:-659253 | None:intergenic |
| AATTGAGTATCTTGCATGTG+AGG | 0.565013 | 1.4:+658339 | MS.gene64018:CDS |
| CAAACCAACAAGAATCATCA+AGG | 0.574004 | 1.4:+659042 | MS.gene64018:CDS |
| AGGTAAAGGTAAAAGCATCC+AGG | 0.575090 | 1.4:+659062 | MS.gene64018:CDS |
| ATTGAGTATCTTGCATGTGA+GGG | 0.575966 | 1.4:+658340 | MS.gene64018:CDS |
| GGATATGCCATTGTTCCTGT+AGG | 0.578642 | 1.4:-658844 | None:intergenic |
| GCAACAACAATATAGAATGA+GGG | 0.579939 | 1.4:+658735 | MS.gene64018:CDS |
| TTCCTGTAGGACGCAACTGG+TGG | 0.593030 | 1.4:-658831 | None:intergenic |
| ACGCCCTTGATTAAAAGTTG+TGG | 0.608515 | 1.4:-658878 | None:intergenic |
| GCAATATCACTTGAGATGCA+TGG | 0.608661 | 1.4:-658714 | None:intergenic |
| TATAATCGCCAAAGAGGACG+TGG | 0.608957 | 1.4:+658907 | MS.gene64018:CDS |
| GGGACTCGACAAGATTCTTG+AGG | 0.610766 | 1.4:+658360 | MS.gene64018:CDS |
| TGTTGATGGAAAGGAAAAGG+AGG | 0.612844 | 1.4:+659194 | MS.gene64018:CDS |
| ACGATCATCATGATTTCTAG+AGG | 0.627654 | 1.4:-659091 | None:intergenic |
| GTCATTGGTCTAAAGCATGT+AGG | 0.629444 | 1.4:+659136 | MS.gene64018:CDS |
| GATGATCGTTGTTATAGATG+CGG | 0.637106 | 1.4:+659105 | MS.gene64018:CDS |
| GAATGATACTACCTGTCTTG+AGG | 0.637144 | 1.4:+659242 | MS.gene64018:CDS |
| CGATCATCATGATTTCTAGA+GGG | 0.638172 | 1.4:-659090 | None:intergenic |
| GGTGGTTATAATCGCCAAAG+AGG | 0.642086 | 1.4:+658901 | MS.gene64018:CDS |
| GTTGCGTCCTACAGGAACAA+TGG | 0.661047 | 1.4:+658837 | MS.gene64018:CDS |
| AAAGGTAAAAGCATCCAGGA+AGG | 0.661440 | 1.4:+659066 | MS.gene64018:CDS |
| TTTGTAATCCATCATCAATG+TGG | 0.715751 | 1.4:-658453 | None:intergenic |
| CGATTTGGGAGATGTCCCAG+AGG | 0.725435 | 1.4:+658387 | MS.gene64018:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATGTTTAAAATTTTGAAT+TGG | - | chr1.4:658285-658304 | None:intergenic | 10.0% |
| !!! | TTCAAAATTTTAAACATAAC+TGG | + | chr1.4:658286-658305 | MS.gene64018:CDS | 15.0% |
| ! | ACAAAATTAAGGCAAGATTT+GGG | + | chr1.4:658515-658534 | MS.gene64018:CDS | 25.0% |
| ! | ATTTACTGAATACTCTGATT+TGG | + | chr1.4:658759-658778 | MS.gene64018:CDS | 25.0% |
| ! | CATAGGAGAAGATATTAATA+TGG | + | chr1.4:659275-659294 | MS.gene64018:CDS | 25.0% |
| ! | GAAGAGGTTATATGAATTAT+AGG | + | chr1.4:659004-659023 | MS.gene64018:intron | 25.0% |
| ! | TATAACTGAAAAGGAAAAGT+TGG | + | chr1.4:658672-658691 | MS.gene64018:CDS | 25.0% |
| !! | AAAAGATCCCAAGATTTTAT+GGG | + | chr1.4:658492-658511 | MS.gene64018:CDS | 25.0% |
| !! | AATTTTGTCCCATAAAATCT+TGG | - | chr1.4:658503-658522 | None:intergenic | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | + | chr1.4:658643-658662 | MS.gene64018:CDS | 25.0% |
| !! | ATTTTGTCCCATAAAATCTT+GGG | - | chr1.4:658502-658521 | None:intergenic | 25.0% |
| !! | GATTTTATGGGACAAAATTA+AGG | + | chr1.4:658504-658523 | MS.gene64018:CDS | 25.0% |
| !! | TATCTTTTACACAGATACTT+TGG | - | chr1.4:659165-659184 | None:intergenic | 25.0% |
| !!! | CAACAACAGTTTTATAATCT+TGG | - | chr1.4:658597-658616 | None:intergenic | 25.0% |
| AACAAGAATCATCAAGGTAA+AGG | + | chr1.4:659048-659067 | MS.gene64018:CDS | 30.0% | |
| AATTCATATAACCTCTTCCA+CGG | - | chr1.4:659002-659021 | None:intergenic | 30.0% | |
| AGATACAAAGAATCTGTTGA+TGG | + | chr1.4:659180-659199 | MS.gene64018:CDS | 30.0% | |
| GACAAAATTAAGGCAAGATT+TGG | + | chr1.4:658514-658533 | MS.gene64018:CDS | 30.0% | |
| GCAACAACAATATAGAATGA+GGG | + | chr1.4:658735-658754 | MS.gene64018:CDS | 30.0% | |
| TGCAACAACAATATAGAATG+AGG | + | chr1.4:658734-658753 | MS.gene64018:CDS | 30.0% | |
| TGGATCAATGGAACAAATTA+AGG | + | chr1.4:658569-658588 | MS.gene64018:CDS | 30.0% | |
| TGGCAAAACTATAACTGAAA+AGG | + | chr1.4:658663-658682 | MS.gene64018:CDS | 30.0% | |
| TTCCATTGATCCATTAATGA+GGG | - | chr1.4:658562-658581 | None:intergenic | 30.0% | |
| TTTGTAATCCATCATCAATG+TGG | - | chr1.4:658456-658475 | None:intergenic | 30.0% | |
| ! | CAAAAGATCCCAAGATTTTA+TGG | + | chr1.4:658491-658510 | MS.gene64018:CDS | 30.0% |
| ! | TTTACCTTGATGATTCTTGT+TGG | - | chr1.4:659049-659068 | None:intergenic | 30.0% |
| AAAGTTGTGGCGTTTATTTC+AGG | - | chr1.4:658868-658887 | None:intergenic | 35.0% | |
| AAGACAGGTAGTATCATTCT+TGG | - | chr1.4:659241-659260 | None:intergenic | 35.0% | |
| AATTGAGTATCTTGCATGTG+AGG | + | chr1.4:658339-658358 | MS.gene64018:CDS | 35.0% | |
| AGACAGGTAGTATCATTCTT+GGG | - | chr1.4:659240-659259 | None:intergenic | 35.0% | |
| ATTGAGTATCTTGCATGTGA+GGG | + | chr1.4:658340-658359 | MS.gene64018:CDS | 35.0% | |
| CAAACCAACAAGAATCATCA+AGG | + | chr1.4:659042-659061 | MS.gene64018:CDS | 35.0% | |
| GATGATCGTTGTTATAGATG+CGG | + | chr1.4:659105-659124 | MS.gene64018:CDS | 35.0% | |
| GTTCCATTGATCCATTAATG+AGG | - | chr1.4:658563-658582 | None:intergenic | 35.0% | |
| TGTTATAGATGCGGAAAGAA+AGG | + | chr1.4:659114-659133 | MS.gene64018:CDS | 35.0% | |
| TTTGGTTACAACTCTTCTAG+TGG | + | chr1.4:658777-658796 | MS.gene64018:CDS | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | + | chr1.4:658874-658893 | MS.gene64018:CDS | 35.0% |
| ! | ACGATCATCATGATTTCTAG+AGG | - | chr1.4:659094-659113 | None:intergenic | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | + | chr1.4:658875-658894 | MS.gene64018:CDS | 35.0% |
| ! | ATCTGTTGATGGAAAGGAAA+AGG | + | chr1.4:659191-659210 | MS.gene64018:CDS | 35.0% |
| ! | CAAAGAATCTGTTGATGGAA+AGG | + | chr1.4:659185-659204 | MS.gene64018:CDS | 35.0% |
| ! | CGATCATCATGATTTCTAGA+GGG | - | chr1.4:659093-659112 | None:intergenic | 35.0% |
| ! | CTCAATTAAGTTGTTGTTCC+AGG | - | chr1.4:658326-658345 | None:intergenic | 35.0% |
| ! | CTTGATGATTCTTGTTGGTT+TGG | - | chr1.4:659044-659063 | None:intergenic | 35.0% |
| ! | TTTTCTTTGCCAATTCCTCT+GGG | - | chr1.4:658405-658424 | None:intergenic | 35.0% |
| ! | TTTTTCTTTGCCAATTCCTC+TGG | - | chr1.4:658406-658425 | None:intergenic | 35.0% |
| !! | AGTCTTATTGCCCTCATTAA+TGG | + | chr1.4:658549-658568 | MS.gene64018:CDS | 35.0% |
| !! | TTGGTTTGGTCATATTTGAG+AGG | - | chr1.4:659030-659049 | None:intergenic | 35.0% |
| ACGCCCTTGATTAAAAGTTG+TGG | - | chr1.4:658881-658900 | None:intergenic | 40.0% | |
| ATCAAGCACCACATTGATGA+TGG | + | chr1.4:658445-658464 | MS.gene64018:CDS | 40.0% | |
| ATTCTTGAGGGAGACGATTT+GGG | + | chr1.4:658373-658392 | MS.gene64018:CDS | 40.0% | |
| GAATGATACTACCTGTCTTG+AGG | + | chr1.4:659242-659261 | MS.gene64018:CDS | 40.0% | |
| GCAATATCACTTGAGATGCA+TGG | - | chr1.4:658717-658736 | None:intergenic | 40.0% | |
| ! | ACAACTTTTAATCAAGGGCG+TGG | + | chr1.4:658880-658899 | MS.gene64018:CDS | 40.0% |
| ! | CATGGTCGAAATCATTTTCG+TGG | + | chr1.4:658970-658989 | MS.gene64018:intron | 40.0% |
| ! | GTCATTGGTCTAAAGCATGT+AGG | + | chr1.4:659136-659155 | MS.gene64018:CDS | 40.0% |
| ! | TGCCCTCATTAATGGATCAA+TGG | + | chr1.4:658557-658576 | MS.gene64018:CDS | 40.0% |
| ! | TGTTGATGGAAAGGAAAAGG+AGG | + | chr1.4:659194-659213 | MS.gene64018:CDS | 40.0% |
| !! | AAAGGTAAAAGCATCCAGGA+AGG | + | chr1.4:659066-659085 | MS.gene64018:CDS | 40.0% |
| !! | AGGTAAAGGTAAAAGCATCC+AGG | + | chr1.4:659062-659081 | MS.gene64018:CDS | 40.0% |
| CTGGAGACAACTACATAACC+TGG | + | chr1.4:658305-658324 | MS.gene64018:CDS | 45.0% | |
| GATGCGGAAAGAAAGGTCAT+TGG | + | chr1.4:659121-659140 | MS.gene64018:CDS | 45.0% | |
| GATTCTTGAGGGAGACGATT+TGG | + | chr1.4:658372-658391 | MS.gene64018:CDS | 45.0% | |
| GGACTCGACAAGATTCTTGA+GGG | + | chr1.4:658361-658380 | MS.gene64018:CDS | 45.0% | |
| GGATATGCCATTGTTCCTGT+AGG | - | chr1.4:658847-658866 | None:intergenic | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | + | chr1.4:658901-658920 | MS.gene64018:CDS | 45.0% | |
| TATAATCGCCAAAGAGGACG+TGG | + | chr1.4:658907-658926 | MS.gene64018:CDS | 45.0% | |
| ! | CGAAATCATTTTCGTGGTGG+AGG | + | chr1.4:658976-658995 | MS.gene64018:intron | 45.0% |
| ! | GGTCGAAATCATTTTCGTGG+TGG | + | chr1.4:658973-658992 | MS.gene64018:intron | 45.0% |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | + | chr1.4:658883-658902 | MS.gene64018:CDS | 45.0% |
| !! | CTTGAGGCTGCTGATTTCAT+AGG | + | chr1.4:659258-659277 | MS.gene64018:CDS | 45.0% |
| GAAATCAGCAGCCTCAAGAC+AGG | - | chr1.4:659256-659275 | None:intergenic | 50.0% | |
| GGGACTCGACAAGATTCTTG+AGG | + | chr1.4:658360-658379 | MS.gene64018:CDS | 50.0% | |
| GTTGCGTCCTACAGGAACAA+TGG | + | chr1.4:658837-658856 | MS.gene64018:CDS | 50.0% | |
| TTGTTCCTGTAGGACGCAAC+TGG | - | chr1.4:658837-658856 | None:intergenic | 50.0% | |
| ! | GATTTCTAGAGGGACCTTCC+TGG | - | chr1.4:659083-659102 | None:intergenic | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | + | chr1.4:658934-658953 | MS.gene64018:intron | 50.0% |
| AACCACCAGTTGCGTCCTAC+AGG | + | chr1.4:658829-658848 | MS.gene64018:CDS | 55.0% | |
| AATCGCCAAAGAGGACGTGG+TGG | + | chr1.4:658910-658929 | MS.gene64018:intron | 55.0% | |
| CATAGCCACCACGTCCTCTT+TGG | - | chr1.4:658918-658937 | None:intergenic | 55.0% | |
| GGGAGATGTCCCAGAGGAAT+TGG | + | chr1.4:658393-658412 | MS.gene64018:CDS | 55.0% | |
| TTCCTGTAGGACGCAACTGG+TGG | - | chr1.4:658834-658853 | None:intergenic | 55.0% | |
| ! | CGATTTGGGAGATGTCCCAG+AGG | + | chr1.4:658387-658406 | MS.gene64018:CDS | 55.0% |
| ! | CGTGGTGGCTATGCTCGTTT+TGG | + | chr1.4:658925-658944 | MS.gene64018:intron | 55.0% |
| ! | GGTGGCTATGCTCGTTTTGG+TGG | + | chr1.4:658928-658947 | MS.gene64018:intron | 55.0% |
| CGTGGTCGAGGCAGAAGTCA+TGG | + | chr1.4:658952-658971 | MS.gene64018:intron | 60.0% | |
| !! | CATTTTCGTGGTGGAGGCCG+TGG | + | chr1.4:658982-659001 | MS.gene64018:intron | 60.0% |
| !! | CGTTTTGGTGGTCGTGGTCG+AGG | + | chr1.4:658940-658959 | MS.gene64018:intron | 60.0% |
| ! | CGTGGTGGAGGCCGTGGAAG+AGG | + | chr1.4:658988-659007 | MS.gene64018:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 658265 | 659302 | 658265 | ID=MS.gene64018 |
| chr1.4 | mRNA | 658265 | 659302 | 658265 | ID=MS.gene64018.t1;Parent=MS.gene64018 |
| chr1.4 | exon | 658265 | 658928 | 658265 | ID=MS.gene64018.t1.exon1;Parent=MS.gene64018.t1 |
| chr1.4 | CDS | 658265 | 658928 | 658265 | ID=cds.MS.gene64018.t1;Parent=MS.gene64018.t1 |
| chr1.4 | exon | 659010 | 659302 | 659010 | ID=MS.gene64018.t1.exon2;Parent=MS.gene64018.t1 |
| chr1.4 | CDS | 659010 | 659302 | 659010 | ID=cds.MS.gene64018.t1;Parent=MS.gene64018.t1 |
| Gene Sequence |
| Protein sequence |
