Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64221.t1 | XP_013689578.1 | 45.8 | 321 | 155 | 5 | 5 | 313 | 6 | 319 | 2.50E-70 | 275.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64221.t1 | A0A2N9I863 | 43.1 | 346 | 159 | 9 | 1 | 316 | 1 | 338 | 1.8e-65 | 259.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060253 | MS.gene64221 | 0.856545 | 2.99E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64221.t1 | MTR_7g062720 | 40.541 | 148 | 80 | 3 | 1 | 146 | 1 | 142 | 8.28e-32 | 117 |
| MS.gene64221.t1 | MTR_4g037355 | 43.220 | 118 | 56 | 4 | 155 | 263 | 1 | 116 | 7.23e-22 | 90.1 |
| MS.gene64221.t1 | MTR_5g061150 | 33.333 | 111 | 74 | 0 | 40 | 150 | 26 | 136 | 3.51e-18 | 80.1 |
| MS.gene64221.t1 | MTR_4g112730 | 57.971 | 69 | 28 | 1 | 143 | 211 | 2 | 69 | 1.75e-17 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 61 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATCAATGGAACAAATTA+AGG | 0.196151 | 1.3:+1076568 | MS.gene64221:CDS |
| AGTCTTATTGCCCTCATTAA+TGG | 0.201947 | 1.3:+1076548 | MS.gene64221:CDS |
| AAAGTTGTGGCGTTTATTTC+AGG | 0.203868 | 1.3:-1076864 | None:intergenic |
| GACAAAATTAAGGCAAGATT+TGG | 0.257188 | 1.3:+1076513 | MS.gene64221:CDS |
| GATTCTTGAGGGAGACGATT+TGG | 0.271296 | 1.3:+1076371 | MS.gene64221:CDS |
| CTCAATTAAGTTGTTGTTCC+AGG | 0.274617 | 1.3:-1076322 | None:intergenic |
| CATAGGAGAAGATATTAATA+TGG | 0.311554 | 1.3:+1077274 | MS.gene64221:CDS |
| ATTTACTGAATACTCTGATT+TGG | 0.313227 | 1.3:+1076758 | MS.gene64221:CDS |
| CTTGATGATTCTTGTTGGTT+TGG | 0.339105 | 1.3:-1077040 | None:intergenic |
| GAAGAGGTTATATGAATTAT+AGG | 0.339571 | 1.3:+1077003 | MS.gene64221:intron |
| GGGAGATGTCCCAGAGGAAT+TGG | 0.345733 | 1.3:+1076392 | MS.gene64221:CDS |
| ATTCTTGAGGGAGACGATTT+GGG | 0.354692 | 1.3:+1076372 | MS.gene64221:CDS |
| AAGACAGGTAGTATCATTCT+TGG | 0.359896 | 1.3:-1077237 | None:intergenic |
| TTTACCTTGATGATTCTTGT+TGG | 0.362649 | 1.3:-1077045 | None:intergenic |
| CTTGAGGCTGCTGATTTCAT+AGG | 0.363536 | 1.3:+1077257 | MS.gene64221:CDS |
| GATTTCTAGAGGGACCTTCC+TGG | 0.373636 | 1.3:-1077079 | None:intergenic |
| CATAGCCACCACGTCCTCTT+TGG | 0.374275 | 1.3:-1076914 | None:intergenic |
| ACAAAATTAAGGCAAGATTT+GGG | 0.416566 | 1.3:+1076514 | MS.gene64221:CDS |
| TATAACTGAAAAGGAAAAGT+TGG | 0.422757 | 1.3:+1076671 | MS.gene64221:CDS |
| TGCCCTCATTAATGGATCAA+TGG | 0.432677 | 1.3:+1076556 | MS.gene64221:CDS |
| AGATACAAAGAATCTGTTGA+TGG | 0.446469 | 1.3:+1077179 | MS.gene64221:CDS |
| TGGCAAAACTATAACTGAAA+AGG | 0.467052 | 1.3:+1076662 | MS.gene64221:CDS |
| ATCTGTTGATGGAAAGGAAA+AGG | 0.477668 | 1.3:+1077190 | MS.gene64221:CDS |
| AACCACCAGTTGCGTCCTAC+AGG | 0.478404 | 1.3:+1076828 | MS.gene64221:CDS |
| TTGTTCCTGTAGGACGCAAC+TGG | 0.480977 | 1.3:-1076833 | None:intergenic |
| AGACAGGTAGTATCATTCTT+GGG | 0.483328 | 1.3:-1077236 | None:intergenic |
| ATCAAGCACCACATTGATGA+TGG | 0.492315 | 1.3:+1076444 | MS.gene64221:CDS |
| TGCAACAACAATATAGAATG+AGG | 0.496917 | 1.3:+1076733 | MS.gene64221:CDS |
| CTGGAGACAACTACATAACC+TGG | 0.502040 | 1.3:+1076304 | MS.gene64221:CDS |
| AACAAGAATCATCAAGGTAA+AGG | 0.504207 | 1.3:+1077047 | MS.gene64221:CDS |
| GTTCCATTGATCCATTAATG+AGG | 0.509644 | 1.3:-1076559 | None:intergenic |
| CAAAGAATCTGTTGATGGAA+AGG | 0.510693 | 1.3:+1077184 | MS.gene64221:CDS |
| TGTTATAGATGCGGAAAGAA+AGG | 0.513070 | 1.3:+1077113 | MS.gene64221:CDS |
| TTCCATTGATCCATTAATGA+GGG | 0.533283 | 1.3:-1076558 | None:intergenic |
| GATGCGGAAAGAAAGGTCAT+TGG | 0.533423 | 1.3:+1077120 | MS.gene64221:CDS |
| TTTGGTTACAACTCTTCTAG+TGG | 0.535203 | 1.3:+1076776 | MS.gene64221:CDS |
| GGACTCGACAAGATTCTTGA+GGG | 0.539594 | 1.3:+1076360 | MS.gene64221:CDS |
| TTGGTTTGGTCATATTTGAG+AGG | 0.544297 | 1.3:-1077026 | None:intergenic |
| GAAATCAGCAGCCTCAAGAC+AGG | 0.558951 | 1.3:-1077252 | None:intergenic |
| AATTGAGTATCTTGCATGTG+AGG | 0.565013 | 1.3:+1076338 | MS.gene64221:CDS |
| CAAACCAACAAGAATCATCA+AGG | 0.574004 | 1.3:+1077041 | MS.gene64221:CDS |
| AGGTAAAGGTAAAAGCATCC+AGG | 0.575090 | 1.3:+1077061 | MS.gene64221:CDS |
| ATTGAGTATCTTGCATGTGA+GGG | 0.575966 | 1.3:+1076339 | MS.gene64221:CDS |
| GGATATGCCATTGTTCCTGT+AGG | 0.578642 | 1.3:-1076843 | None:intergenic |
| GCAACAACAATATAGAATGA+GGG | 0.579939 | 1.3:+1076734 | MS.gene64221:CDS |
| TTCCTGTAGGACGCAACTGG+TGG | 0.593030 | 1.3:-1076830 | None:intergenic |
| ACGCCCTTGATTAAAAGTTG+TGG | 0.608515 | 1.3:-1076877 | None:intergenic |
| GCAATATCACTTGAGATGCA+TGG | 0.608661 | 1.3:-1076713 | None:intergenic |
| TATAATCGCCAAAGAGGACG+TGG | 0.608957 | 1.3:+1076906 | MS.gene64221:CDS |
| GGGACTCGACAAGATTCTTG+AGG | 0.610766 | 1.3:+1076359 | MS.gene64221:CDS |
| TGTTGATGGAAAGGAAAAGG+AGG | 0.612844 | 1.3:+1077193 | MS.gene64221:CDS |
| ACGATCATCATGATTTCTAG+AGG | 0.627654 | 1.3:-1077090 | None:intergenic |
| GTCATTGGTCTAAAGCATGT+AGG | 0.629444 | 1.3:+1077135 | MS.gene64221:CDS |
| GATGATCGTTGTTATAGATG+CGG | 0.637106 | 1.3:+1077104 | MS.gene64221:CDS |
| GAATGATACTACCTGTCTTG+AGG | 0.637144 | 1.3:+1077241 | MS.gene64221:CDS |
| CGATCATCATGATTTCTAGA+GGG | 0.638172 | 1.3:-1077089 | None:intergenic |
| GGTGGTTATAATCGCCAAAG+AGG | 0.642086 | 1.3:+1076900 | MS.gene64221:CDS |
| GTTGCGTCCTACAGGAACAA+TGG | 0.661047 | 1.3:+1076836 | MS.gene64221:CDS |
| AAAGGTAAAAGCATCCAGGA+AGG | 0.661440 | 1.3:+1077065 | MS.gene64221:CDS |
| TTTGTAATCCATCATCAATG+TGG | 0.715751 | 1.3:-1076452 | None:intergenic |
| CGATTTGGGAGATGTCCCAG+AGG | 0.725435 | 1.3:+1076386 | MS.gene64221:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATGTTTAAAATTTTGAAT+TGG | - | chr1.3:1076284-1076303 | None:intergenic | 10.0% |
| !!! | TTCAAAATTTTAAACATAAC+TGG | + | chr1.3:1076285-1076304 | MS.gene64221:CDS | 15.0% |
| ! | ACAAAATTAAGGCAAGATTT+GGG | + | chr1.3:1076514-1076533 | MS.gene64221:CDS | 25.0% |
| ! | ATTTACTGAATACTCTGATT+TGG | + | chr1.3:1076758-1076777 | MS.gene64221:CDS | 25.0% |
| ! | CATAGGAGAAGATATTAATA+TGG | + | chr1.3:1077274-1077293 | MS.gene64221:CDS | 25.0% |
| ! | GAAGAGGTTATATGAATTAT+AGG | + | chr1.3:1077003-1077022 | MS.gene64221:intron | 25.0% |
| ! | TATAACTGAAAAGGAAAAGT+TGG | + | chr1.3:1076671-1076690 | MS.gene64221:CDS | 25.0% |
| !! | AAAAGATCCCAAGATTTTAT+GGG | + | chr1.3:1076491-1076510 | MS.gene64221:CDS | 25.0% |
| !! | AATTTTGTCCCATAAAATCT+TGG | - | chr1.3:1076502-1076521 | None:intergenic | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | + | chr1.3:1076642-1076661 | MS.gene64221:CDS | 25.0% |
| !! | ATTTTGTCCCATAAAATCTT+GGG | - | chr1.3:1076501-1076520 | None:intergenic | 25.0% |
| !! | GATTTTATGGGACAAAATTA+AGG | + | chr1.3:1076503-1076522 | MS.gene64221:CDS | 25.0% |
| !! | TATCTTTTACACAGATACTT+TGG | - | chr1.3:1077164-1077183 | None:intergenic | 25.0% |
| !!! | CAACAACAGTTTTATAATCT+TGG | - | chr1.3:1076596-1076615 | None:intergenic | 25.0% |
| AACAAGAATCATCAAGGTAA+AGG | + | chr1.3:1077047-1077066 | MS.gene64221:CDS | 30.0% | |
| AATTCATATAACCTCTTCCA+CGG | - | chr1.3:1077001-1077020 | None:intergenic | 30.0% | |
| AGATACAAAGAATCTGTTGA+TGG | + | chr1.3:1077179-1077198 | MS.gene64221:CDS | 30.0% | |
| GACAAAATTAAGGCAAGATT+TGG | + | chr1.3:1076513-1076532 | MS.gene64221:CDS | 30.0% | |
| GCAACAACAATATAGAATGA+GGG | + | chr1.3:1076734-1076753 | MS.gene64221:CDS | 30.0% | |
| TGCAACAACAATATAGAATG+AGG | + | chr1.3:1076733-1076752 | MS.gene64221:CDS | 30.0% | |
| TGGATCAATGGAACAAATTA+AGG | + | chr1.3:1076568-1076587 | MS.gene64221:CDS | 30.0% | |
| TGGCAAAACTATAACTGAAA+AGG | + | chr1.3:1076662-1076681 | MS.gene64221:CDS | 30.0% | |
| TTCCATTGATCCATTAATGA+GGG | - | chr1.3:1076561-1076580 | None:intergenic | 30.0% | |
| TTTGTAATCCATCATCAATG+TGG | - | chr1.3:1076455-1076474 | None:intergenic | 30.0% | |
| ! | CAAAAGATCCCAAGATTTTA+TGG | + | chr1.3:1076490-1076509 | MS.gene64221:CDS | 30.0% |
| ! | TTTACCTTGATGATTCTTGT+TGG | - | chr1.3:1077048-1077067 | None:intergenic | 30.0% |
| AAAGTTGTGGCGTTTATTTC+AGG | - | chr1.3:1076867-1076886 | None:intergenic | 35.0% | |
| AAGACAGGTAGTATCATTCT+TGG | - | chr1.3:1077240-1077259 | None:intergenic | 35.0% | |
| AATTGAGTATCTTGCATGTG+AGG | + | chr1.3:1076338-1076357 | MS.gene64221:CDS | 35.0% | |
| AGACAGGTAGTATCATTCTT+GGG | - | chr1.3:1077239-1077258 | None:intergenic | 35.0% | |
| ATTGAGTATCTTGCATGTGA+GGG | + | chr1.3:1076339-1076358 | MS.gene64221:CDS | 35.0% | |
| CAAACCAACAAGAATCATCA+AGG | + | chr1.3:1077041-1077060 | MS.gene64221:CDS | 35.0% | |
| GATGATCGTTGTTATAGATG+CGG | + | chr1.3:1077104-1077123 | MS.gene64221:CDS | 35.0% | |
| GTTCCATTGATCCATTAATG+AGG | - | chr1.3:1076562-1076581 | None:intergenic | 35.0% | |
| TGTTATAGATGCGGAAAGAA+AGG | + | chr1.3:1077113-1077132 | MS.gene64221:CDS | 35.0% | |
| TTTGGTTACAACTCTTCTAG+TGG | + | chr1.3:1076776-1076795 | MS.gene64221:CDS | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | + | chr1.3:1076873-1076892 | MS.gene64221:CDS | 35.0% |
| ! | ACGATCATCATGATTTCTAG+AGG | - | chr1.3:1077093-1077112 | None:intergenic | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | + | chr1.3:1076874-1076893 | MS.gene64221:CDS | 35.0% |
| ! | ATCTGTTGATGGAAAGGAAA+AGG | + | chr1.3:1077190-1077209 | MS.gene64221:CDS | 35.0% |
| ! | CAAAGAATCTGTTGATGGAA+AGG | + | chr1.3:1077184-1077203 | MS.gene64221:CDS | 35.0% |
| ! | CGATCATCATGATTTCTAGA+GGG | - | chr1.3:1077092-1077111 | None:intergenic | 35.0% |
| ! | CTCAATTAAGTTGTTGTTCC+AGG | - | chr1.3:1076325-1076344 | None:intergenic | 35.0% |
| ! | CTTGATGATTCTTGTTGGTT+TGG | - | chr1.3:1077043-1077062 | None:intergenic | 35.0% |
| ! | TTTTCTTTGCCAATTCCTCT+GGG | - | chr1.3:1076404-1076423 | None:intergenic | 35.0% |
| ! | TTTTTCTTTGCCAATTCCTC+TGG | - | chr1.3:1076405-1076424 | None:intergenic | 35.0% |
| !! | AGTCTTATTGCCCTCATTAA+TGG | + | chr1.3:1076548-1076567 | MS.gene64221:CDS | 35.0% |
| !! | TTGGTTTGGTCATATTTGAG+AGG | - | chr1.3:1077029-1077048 | None:intergenic | 35.0% |
| ACGCCCTTGATTAAAAGTTG+TGG | - | chr1.3:1076880-1076899 | None:intergenic | 40.0% | |
| ATCAAGCACCACATTGATGA+TGG | + | chr1.3:1076444-1076463 | MS.gene64221:CDS | 40.0% | |
| ATTCTTGAGGGAGACGATTT+GGG | + | chr1.3:1076372-1076391 | MS.gene64221:CDS | 40.0% | |
| GAATGATACTACCTGTCTTG+AGG | + | chr1.3:1077241-1077260 | MS.gene64221:CDS | 40.0% | |
| GCAATATCACTTGAGATGCA+TGG | - | chr1.3:1076716-1076735 | None:intergenic | 40.0% | |
| ! | ACAACTTTTAATCAAGGGCG+TGG | + | chr1.3:1076879-1076898 | MS.gene64221:CDS | 40.0% |
| ! | CATGGTCGAAATCATTTTCG+TGG | + | chr1.3:1076969-1076988 | MS.gene64221:intron | 40.0% |
| ! | GTCATTGGTCTAAAGCATGT+AGG | + | chr1.3:1077135-1077154 | MS.gene64221:CDS | 40.0% |
| ! | TGCCCTCATTAATGGATCAA+TGG | + | chr1.3:1076556-1076575 | MS.gene64221:CDS | 40.0% |
| ! | TGTTGATGGAAAGGAAAAGG+AGG | + | chr1.3:1077193-1077212 | MS.gene64221:CDS | 40.0% |
| !! | AAAGGTAAAAGCATCCAGGA+AGG | + | chr1.3:1077065-1077084 | MS.gene64221:CDS | 40.0% |
| !! | AGGTAAAGGTAAAAGCATCC+AGG | + | chr1.3:1077061-1077080 | MS.gene64221:CDS | 40.0% |
| CTGGAGACAACTACATAACC+TGG | + | chr1.3:1076304-1076323 | MS.gene64221:CDS | 45.0% | |
| GATGCGGAAAGAAAGGTCAT+TGG | + | chr1.3:1077120-1077139 | MS.gene64221:CDS | 45.0% | |
| GATTCTTGAGGGAGACGATT+TGG | + | chr1.3:1076371-1076390 | MS.gene64221:CDS | 45.0% | |
| GGACTCGACAAGATTCTTGA+GGG | + | chr1.3:1076360-1076379 | MS.gene64221:CDS | 45.0% | |
| GGATATGCCATTGTTCCTGT+AGG | - | chr1.3:1076846-1076865 | None:intergenic | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | + | chr1.3:1076900-1076919 | MS.gene64221:CDS | 45.0% | |
| TATAATCGCCAAAGAGGACG+TGG | + | chr1.3:1076906-1076925 | MS.gene64221:CDS | 45.0% | |
| ! | CGAAATCATTTTCGTGGTGG+AGG | + | chr1.3:1076975-1076994 | MS.gene64221:intron | 45.0% |
| ! | GGTCGAAATCATTTTCGTGG+TGG | + | chr1.3:1076972-1076991 | MS.gene64221:intron | 45.0% |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | + | chr1.3:1076882-1076901 | MS.gene64221:CDS | 45.0% |
| !! | CTTGAGGCTGCTGATTTCAT+AGG | + | chr1.3:1077257-1077276 | MS.gene64221:CDS | 45.0% |
| GAAATCAGCAGCCTCAAGAC+AGG | - | chr1.3:1077255-1077274 | None:intergenic | 50.0% | |
| GGGACTCGACAAGATTCTTG+AGG | + | chr1.3:1076359-1076378 | MS.gene64221:CDS | 50.0% | |
| GTTGCGTCCTACAGGAACAA+TGG | + | chr1.3:1076836-1076855 | MS.gene64221:CDS | 50.0% | |
| TTGTTCCTGTAGGACGCAAC+TGG | - | chr1.3:1076836-1076855 | None:intergenic | 50.0% | |
| ! | GATTTCTAGAGGGACCTTCC+TGG | - | chr1.3:1077082-1077101 | None:intergenic | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | + | chr1.3:1076933-1076952 | MS.gene64221:intron | 50.0% |
| AACCACCAGTTGCGTCCTAC+AGG | + | chr1.3:1076828-1076847 | MS.gene64221:CDS | 55.0% | |
| AATCGCCAAAGAGGACGTGG+TGG | + | chr1.3:1076909-1076928 | MS.gene64221:intron | 55.0% | |
| CATAGCCACCACGTCCTCTT+TGG | - | chr1.3:1076917-1076936 | None:intergenic | 55.0% | |
| GGGAGATGTCCCAGAGGAAT+TGG | + | chr1.3:1076392-1076411 | MS.gene64221:CDS | 55.0% | |
| TTCCTGTAGGACGCAACTGG+TGG | - | chr1.3:1076833-1076852 | None:intergenic | 55.0% | |
| ! | CGATTTGGGAGATGTCCCAG+AGG | + | chr1.3:1076386-1076405 | MS.gene64221:CDS | 55.0% |
| ! | CGTGGTGGCTATGCTCGTTT+TGG | + | chr1.3:1076924-1076943 | MS.gene64221:intron | 55.0% |
| ! | GGTGGCTATGCTCGTTTTGG+TGG | + | chr1.3:1076927-1076946 | MS.gene64221:intron | 55.0% |
| CGTGGTCGAGGCAGAAGTCA+TGG | + | chr1.3:1076951-1076970 | MS.gene64221:intron | 60.0% | |
| !! | CATTTTCGTGGTGGAGGCCG+TGG | + | chr1.3:1076981-1077000 | MS.gene64221:intron | 60.0% |
| !! | CGTTTTGGTGGTCGTGGTCG+AGG | + | chr1.3:1076939-1076958 | MS.gene64221:intron | 60.0% |
| ! | CGTGGTGGAGGCCGTGGAAG+AGG | + | chr1.3:1076987-1077006 | MS.gene64221:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 1076264 | 1077301 | 1076264 | ID=MS.gene64221 |
| chr1.3 | mRNA | 1076264 | 1077301 | 1076264 | ID=MS.gene64221.t1;Parent=MS.gene64221 |
| chr1.3 | exon | 1076264 | 1076927 | 1076264 | ID=MS.gene64221.t1.exon1;Parent=MS.gene64221.t1 |
| chr1.3 | CDS | 1076264 | 1076927 | 1076264 | ID=cds.MS.gene64221.t1;Parent=MS.gene64221.t1 |
| chr1.3 | exon | 1077009 | 1077301 | 1077009 | ID=MS.gene64221.t1.exon2;Parent=MS.gene64221.t1 |
| chr1.3 | CDS | 1077009 | 1077301 | 1077009 | ID=cds.MS.gene64221.t1;Parent=MS.gene64221.t1 |
| Gene Sequence |
| Protein sequence |
