AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65373

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65373.t1 MTR_5g099010 95.385 585 22 2 1 585 1 580 0.0 1162
MS.gene65373.t1 MTR_3g115220 65.833 480 155 3 41 511 20 499 0.0 677
MS.gene65373.t1 MTR_4g100810 60.140 429 167 3 95 520 79 506 0.0 546
MS.gene65373.t1 MTR_5g012770 55.180 473 187 6 40 505 41 495 7.68e-180 518
MS.gene65373.t1 MTR_1g031590 36.863 510 289 12 21 505 46 547 6.80e-100 314
MS.gene65373.t1 MTR_5g042200 45.316 395 196 8 127 505 88 478 2.15e-98 308
MS.gene65373.t1 MTR_4g084570 36.595 511 291 12 20 505 46 548 5.31e-97 307
MS.gene65373.t1 MTR_4g082310 42.459 431 222 10 113 523 80 504 7.12e-97 305
MS.gene65373.t1 MTR_5g064620 42.202 436 219 13 96 506 86 513 1.36e-96 305
MS.gene65373.t1 MTR_3g099480 40.217 460 246 11 19 459 27 476 2.82e-95 302
MS.gene65373.t1 MTR_3g096020 38.230 531 283 13 7 514 85 593 6.68e-94 300
MS.gene65373.t1 MTR_6g016015 42.347 392 209 7 127 503 118 507 3.96e-93 295
MS.gene65373.t1 MTR_2g016470 37.013 462 233 12 96 505 160 615 3.57e-92 296
MS.gene65373.t1 MTR_4g019210 38.462 455 255 14 75 514 187 631 5.84e-92 296
MS.gene65373.t1 MTR_3g005340 38.979 431 235 9 97 506 123 546 7.42e-92 294
MS.gene65373.t1 MTR_5g059260 43.869 367 184 7 127 474 160 523 1.72e-89 288
MS.gene65373.t1 MTR_5g081690 35.542 498 293 13 21 505 29 511 2.64e-89 286
MS.gene65373.t1 MTR_4g125460 37.705 427 249 9 95 509 62 483 4.35e-89 284
MS.gene65373.t1 MTR_4g110030 41.944 391 212 7 127 503 119 508 1.99e-88 283
MS.gene65373.t1 MTR_4g125460 37.995 429 245 11 95 509 201 622 3.83e-87 283
MS.gene65373.t1 MTR_2g059350 37.986 437 238 10 95 503 76 507 1.62e-86 278
MS.gene65373.t1 MTR_3g086570 39.950 398 218 11 127 505 153 548 1.15e-85 277
MS.gene65373.t1 MTR_5g032100 36.504 452 237 13 95 505 60 502 1.08e-80 263
MS.gene65373.t1 MTR_4g132590 38.442 398 194 8 127 475 68 463 1.28e-78 257
MS.gene65373.t1 MTR_3g100310 33.855 511 268 17 48 505 23 516 1.61e-78 257
MS.gene65373.t1 MTR_4g132590 38.442 398 194 8 127 475 167 562 1.06e-77 257
MS.gene65373.t1 MTR_3g026650 35.945 434 259 9 89 510 136 562 8.33e-77 254
MS.gene65373.t1 MTR_1g102030 35.648 432 254 10 95 508 61 486 1.14e-74 247
MS.gene65373.t1 MTR_4g023030 35.963 431 257 9 89 507 196 619 1.51e-70 239
MS.gene65373.t1 MTR_3g026650 37.640 356 208 5 89 435 136 486 1.26e-65 222
MS.gene65373.t1 MTR_3g086570 40.647 278 148 7 127 387 153 430 7.23e-61 208
MS.gene65373.t1 MTR_1g101990 38.028 284 160 8 234 508 6 282 3.43e-53 184
MS.gene65373.t1 MTR_7g017330 31.152 382 231 11 40 399 19 390 7.18e-42 160
MS.gene65373.t1 MTR_4g010990 30.178 338 198 9 127 448 78 393 3.02e-38 147
MS.gene65373.t1 MTR_3g026650 37.387 222 135 3 89 308 136 355 3.43e-38 145
MS.gene65373.t1 MTR_7g064210 44.382 178 85 3 226 391 13 188 1.52e-34 135
MS.gene65373.t1 MTR_4g010990 29.538 325 191 9 140 448 2 304 1.79e-34 134
MS.gene65373.t1 MTR_3g092590 28.831 385 251 11 37 403 7 386 8.17e-34 136
MS.gene65373.t1 MTR_3g092590 28.682 387 249 11 37 403 7 386 8.82e-34 136
MS.gene65373.t1 MTR_1g007390 32.472 271 162 8 152 403 124 392 2.40e-29 123
MS.gene65373.t1 MTR_2g038320 24.518 363 222 10 127 471 95 423 1.06e-20 95.1
MS.gene65373.t1 MTR_4g008200 26.113 337 204 12 125 450 36 338 3.13e-19 90.1
MS.gene65373.t1 MTR_4g008200 26.113 337 204 12 125 450 87 389 5.03e-19 90.1
MS.gene65373.t1 MTR_1g101985 40.385 104 60 1 95 198 61 162 5.55e-19 85.5
MS.gene65373.t1 MTR_1g095680 43.182 88 39 2 246 322 7 94 1.34e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65373.t1 AT1G76270 58.645 561 220 5 34 585 15 572 0.0 677
MS.gene65373.t1 AT4G38390 63.158 494 176 3 41 531 42 532 0.0 670
MS.gene65373.t1 AT1G20550 57.793 571 218 7 30 585 2 564 0.0 663
MS.gene65373.t1 AT4G16650 62.708 421 154 2 95 513 93 512 0.0 538
MS.gene65373.t1 AT4G16650 62.708 421 154 2 95 513 93 512 0.0 538
MS.gene65373.t1 AT2G01480 44.321 361 183 7 127 470 157 516 2.91e-97 308
MS.gene65373.t1 AT2G01480 44.633 354 179 6 127 463 157 510 1.27e-96 305
MS.gene65373.t1 AT1G22460 43.765 409 201 10 127 512 163 565 9.85e-96 304
MS.gene65373.t1 AT5G64600 37.087 515 270 13 29 506 19 516 2.88e-95 301
MS.gene65373.t1 AT1G14970 40.955 398 214 9 127 505 12 407 1.49e-92 291
MS.gene65373.t1 AT1G14970 40.955 398 214 9 127 505 38 433 2.87e-92 291
MS.gene65373.t1 AT1G35510 36.106 529 291 14 22 514 49 566 3.12e-92 295
MS.gene65373.t1 AT1G38065 42.268 388 201 7 110 475 17 403 4.10e-92 291
MS.gene65373.t1 AT1G14020 42.640 394 210 7 128 507 95 486 1.11e-91 291
MS.gene65373.t1 AT1G38065 43.716 366 188 6 127 475 29 393 1.83e-91 289
MS.gene65373.t1 AT1G14970 40.955 398 214 9 127 505 158 553 7.94e-91 291
MS.gene65373.t1 AT2G03280 42.967 391 206 8 128 503 92 480 2.86e-90 287
MS.gene65373.t1 AT5G35570 40.654 428 235 11 96 510 225 646 3.57e-90 291
MS.gene65373.t1 AT1G38131 43.716 366 188 6 127 475 158 522 7.55e-90 289
MS.gene65373.t1 AT2G37980 39.450 436 231 12 95 510 199 621 5.28e-89 288
MS.gene65373.t1 AT5G15740 41.117 394 211 7 127 503 109 498 1.09e-88 283
MS.gene65373.t1 AT3G54100 34.630 514 293 14 21 510 123 617 2.34e-87 284
MS.gene65373.t1 AT3G02250 40.921 391 216 7 127 503 109 498 7.91e-87 279
MS.gene65373.t1 AT1G29200 39.480 423 209 8 98 475 44 464 1.71e-85 275
MS.gene65373.t1 AT1G29200 39.480 423 209 8 98 475 44 464 1.71e-85 275
MS.gene65373.t1 AT2G03280 40.191 418 206 9 128 503 92 507 1.90e-84 273
MS.gene65373.t1 AT1G29200 39.480 423 209 8 98 475 160 580 2.39e-84 275
MS.gene65373.t1 AT3G26370 39.447 398 220 11 127 505 161 556 3.90e-84 273
MS.gene65373.t1 AT1G29200 41.071 392 184 8 129 475 1 390 9.63e-84 268
MS.gene65373.t1 AT5G65470 37.885 454 230 13 95 505 59 503 1.93e-83 270
MS.gene65373.t1 AT1G11990 40.872 367 199 6 127 475 193 559 1.44e-81 267
MS.gene65373.t1 AT1G62330 37.093 399 199 7 127 475 224 620 1.88e-81 269
MS.gene65373.t1 AT1G04910 36.277 419 255 7 95 505 61 475 4.15e-80 261
MS.gene65373.t1 AT4G24530 35.412 449 246 12 95 505 75 517 1.26e-79 260
MS.gene65373.t1 AT5G01100 31.407 519 303 15 39 519 120 623 8.48e-78 258
MS.gene65373.t1 AT1G11990 37.376 404 198 7 127 475 178 581 1.95e-75 252
MS.gene65373.t1 AT2G44500 35.714 420 260 6 95 506 146 563 5.54e-75 249
MS.gene65373.t1 AT5G63390 36.318 402 246 5 95 489 133 531 1.42e-74 248
MS.gene65373.t1 AT3G07900 36.131 429 258 8 94 511 156 579 1.35e-71 241
MS.gene65373.t1 AT1G52630 30.366 382 221 12 77 441 30 383 6.95e-43 159
MS.gene65373.t1 AT3G30300 31.500 400 228 14 23 400 18 393 1.94e-41 159
MS.gene65373.t1 AT3G30300 31.500 400 228 14 23 400 18 393 1.94e-41 159
MS.gene65373.t1 AT3G03810 34.769 325 177 9 98 403 77 385 1.57e-39 154
MS.gene65373.t1 AT3G03810 34.769 325 177 9 98 403 77 385 1.57e-39 154
MS.gene65373.t1 AT1G52630 31.962 316 179 9 140 441 2 295 4.82e-38 144
MS.gene65373.t1 AT3G26370 38.756 209 117 6 127 324 161 369 8.52e-38 144
MS.gene65373.t1 AT2G44500 33.846 260 165 3 95 348 146 404 6.25e-36 140

Find 155 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCATCGCGCTACATTTA+AGG 0.117490 5.2:-83746908 MS.gene65373:intron
GTTATTCTTGTTCATGAATA+AGG 0.127801 5.2:+83745967 None:intergenic
GCATTCTCTGGTTGCTATTA+TGG 0.145988 5.2:-83746654 MS.gene65373:CDS
TGAAGAGCATTTACAATAAA+AGG 0.154073 5.2:+83748840 None:intergenic
AATTGGATCATACATCTTTC+TGG 0.170645 5.2:-83747604 MS.gene65373:CDS
ACAAATTTCTCTCCCATATT+TGG 0.187446 5.2:+83747358 None:intergenic
CAAATTTACGGATTCGATTC+AGG 0.201483 5.2:-83746976 MS.gene65373:CDS
TGTAAAGCAGCTACCAAATA+TGG 0.220138 5.2:-83747371 MS.gene65373:CDS
CTTGGAACACGCACTGTATA+TGG 0.243424 5.2:+83747331 None:intergenic
ATGCTTAGAGCACTAGGTTT+TGG 0.277255 5.2:-83746416 MS.gene65373:CDS
CTACCATGCTCTCAAATTTA+CGG 0.290291 5.2:-83746988 MS.gene65373:CDS
CCATGTCTTCTTACTTTCTC+AGG 0.302140 5.2:+83746473 None:intergenic
TTATTCTTGTTCATGAATAA+GGG 0.305017 5.2:+83745968 None:intergenic
TTCTTTCTCTTCACTGATGA+TGG 0.313084 5.2:+83749014 None:intergenic
ACGCGACCCTCGTAGCATTT+GGG 0.325695 5.2:+83747298 None:intergenic
GAAAAGAACTTGGTGAAATT+AGG 0.326334 5.2:-83746614 MS.gene65373:CDS
TACTCTTGTTATTCCAGAAT+TGG 0.327689 5.2:-83747621 MS.gene65373:CDS
AAGGAGTCCGATGAGTCTTC+TGG 0.328208 5.2:+83748968 None:intergenic
ACGCATTTGTATGTGGCTTC+TGG 0.336705 5.2:-83746386 MS.gene65373:CDS
TGTATTTCAGGAAAATGATT+TGG 0.337250 5.2:-83748728 MS.gene65373:intron
AACGCGACCCTCGTAGCATT+TGG 0.337594 5.2:+83747297 None:intergenic
CTACTTACAGGCATGATCTA+TGG 0.340612 5.2:-83748555 MS.gene65373:CDS
ATCTCGAGTTCGAACTTTCC+AGG 0.350555 5.2:-83745924 MS.gene65373:CDS
AAAGATGTATGATCCAATTC+TGG 0.354641 5.2:+83747608 None:intergenic
GTATCGATCCGGATGAGTTT+TGG 0.355722 5.2:+83748357 None:intergenic
AACTTTCCAGGTAGGCTTCA+TGG 0.360105 5.2:-83745912 MS.gene65373:CDS
GTCTGAATTAGATTATGATT+TGG 0.365270 5.2:-83745705 MS.gene65373:CDS
GAGACGACGGCGAAGGAAAC+TGG 0.366522 5.2:+83748941 None:intergenic
CTTAGCTACCAAGGAGGAAT+TGG 0.368531 5.2:-83746291 MS.gene65373:CDS
GTCTCCTTTGAATGGAAGTT+GGG 0.371214 5.2:+83746314 None:intergenic
CTTGGTCAGTACCCATTAGA+AGG 0.376857 5.2:+83745630 None:intergenic
AAGAGTAGCATTCAAAAGAT+AGG 0.384573 5.2:+83747637 None:intergenic
AGCATAAGACCAACTTCCTC+TGG 0.394026 5.2:+83746434 None:intergenic
CTCAATTATTTGTTGCCGAT+TGG 0.396141 5.2:-83747426 MS.gene65373:CDS
CCGGATATGCTTGCATTCTC+TGG 0.396269 5.2:-83746666 MS.gene65373:CDS
TCAAACGCATGAAGGCAATA+AGG 0.398688 5.2:-83745774 MS.gene65373:CDS
CCAGAGAATGCAAGCATATC+CGG 0.399860 5.2:+83746666 None:intergenic
GGCTTAAATCAACAGAGAAC+AGG 0.407017 5.2:-83748313 MS.gene65373:CDS
GCAATCGCCATTTCACATTC+CGG 0.408047 5.2:-83748677 MS.gene65373:intron
AGGCAAATGAAGCTACTTAC+AGG 0.416698 5.2:-83748567 MS.gene65373:CDS
TCAGGGAATGGGAAATTTGT+TGG 0.419141 5.2:-83746958 MS.gene65373:CDS
GTGGCTTCTGGAGAAATATA+TGG 0.419254 5.2:-83746374 MS.gene65373:CDS
ATCTACAAAAGCTGAGGTGC+AGG 0.420709 5.2:-83747016 MS.gene65373:CDS
CTGCCTGACCTCAACTCATT+AGG 0.423475 5.2:+83745884 None:intergenic
GAAGAGCATTTACAATAAAA+GGG 0.426667 5.2:+83748841 None:intergenic
CTACAGACTTTCTAATCTGC+TGG 0.431795 5.2:-83747045 MS.gene65373:CDS
AAATTTACGGATTCGATTCA+GGG 0.431803 5.2:-83746975 MS.gene65373:CDS
AGAGAGCTCTGAGGGGTGCT+AGG 0.437137 5.2:+83746339 None:intergenic
CTCTGGTTGCTATTATGGTG+GGG 0.437730 5.2:-83746649 MS.gene65373:CDS
CTGTTGATTTAAGCCTCCAC+TGG 0.440491 5.2:+83748321 None:intergenic
TACGGATTCGATTCAGGGAA+TGG 0.440840 5.2:-83746970 MS.gene65373:CDS
CTCTGTTGTAGGAGGTACTT+TGG 0.443197 5.2:-83746031 MS.gene65373:intron
GGTCTCCTTTGAATGGAAGT+TGG 0.455130 5.2:+83746313 None:intergenic
TCTCTGGTTGCTATTATGGT+GGG 0.455204 5.2:-83746650 MS.gene65373:CDS
ATTGCATTACACAGGTTGTT+CGG 0.456755 5.2:-83747082 MS.gene65373:intron
CGTGAAGAGAAAGACACAAA+TGG 0.458606 5.2:+83746266 None:intergenic
TTCTCTGCCTGTAGTAATGC+CGG 0.460147 5.2:-83748511 MS.gene65373:CDS
TGTTTCATATAGGTTTGAAC+CGG 0.461614 5.2:-83746685 MS.gene65373:intron
TAGCTAAGGTCTCCTTTGAA+TGG 0.465721 5.2:+83746306 None:intergenic
ACGGATTCGATTCAGGGAAT+GGG 0.465732 5.2:-83746969 MS.gene65373:CDS
TTCTCTGGTTGCTATTATGG+TGG 0.468408 5.2:-83746651 MS.gene65373:CDS
CATCTTTCTGGAAAGACAAC+AGG 0.468808 5.2:-83747592 MS.gene65373:intron
GTGAAGAGAAAGACACAAAT+GGG 0.470665 5.2:+83746267 None:intergenic
GCTCTCCCATGAAGCCTACC+TGG 0.479446 5.2:+83745906 None:intergenic
CAATTCCTCCTTGGTAGCTA+AGG 0.492942 5.2:+83746292 None:intergenic
CCAACTTCCTCTGGGGTAAG+TGG 0.493118 5.2:+83746443 None:intergenic
TTTGGACAATTATAGAAAGC+AGG 0.493179 5.2:-83745687 MS.gene65373:CDS
TCTGGTTGCTATTATGGTGG+GGG 0.493967 5.2:-83746648 MS.gene65373:CDS
GGTCTTATGCTTAGAGCACT+AGG 0.494580 5.2:-83746422 MS.gene65373:CDS
CTAAAATTCTAGCTGGAAGA+AGG 0.494617 5.2:-83746172 MS.gene65373:intron
ACTTTCCAGGTAGGCTTCAT+GGG 0.496124 5.2:-83745911 MS.gene65373:CDS
GGCTAATACCAAAACTCATC+CGG 0.498226 5.2:-83748365 MS.gene65373:CDS
CTCGTACTTCCTCAGCAGGC+TGG 0.501222 5.2:+83745732 None:intergenic
ACACGCTTCTTGACCAGCAC+TGG 0.503067 5.2:+83747271 None:intergenic
TGTGTCTTTCTCTTCACGCA+TGG 0.504664 5.2:-83746261 MS.gene65373:CDS
AAGGCAATAAGGTAGAAAAC+AGG 0.505309 5.2:-83745763 MS.gene65373:CDS
GAGCCTAATGAGTTGAGGTC+AGG 0.509212 5.2:-83745887 MS.gene65373:CDS
AAAGAGAAGGCAAACGGAGA+TGG 0.510159 5.2:+83748896 None:intergenic
CGCGACCCTCGTAGCATTTG+GGG 0.512807 5.2:+83747299 None:intergenic
GTAAAGCAGCTACCAAATAT+GGG 0.514532 5.2:-83747370 MS.gene65373:CDS
TTGATTACCGGAATGTGAAA+TGG 0.514677 5.2:+83748670 None:intergenic
AGAATACTTGCACTTCAGCT+TGG 0.514937 5.2:+83745612 None:intergenic
CTTCCCCAACTTCCATTCAA+AGG 0.524406 5.2:-83746318 MS.gene65373:CDS
AAGGGATGTTATAAACCAAT+CGG 0.525670 5.2:+83747411 None:intergenic
CATATACAGTGCGTGTTCCA+AGG 0.527953 5.2:-83747330 MS.gene65373:CDS
CCTGAGAAAGTAAGAAGACA+TGG 0.528230 5.2:-83746473 MS.gene65373:CDS
GTTGGGGAAGAGAGCTCTGA+GGG 0.529842 5.2:+83746331 None:intergenic
CACTCTCGTACTTCCTCAGC+AGG 0.530274 5.2:+83745728 None:intergenic
ATTGGTTTATAACATCCCTT+CGG 0.534376 5.2:-83747408 MS.gene65373:CDS
GAAAAGGAAAGAAAAGAACT+TGG 0.534475 5.2:-83746624 MS.gene65373:CDS
GCATAAGACCAACTTCCTCT+GGG 0.536685 5.2:+83746435 None:intergenic
GAAAAGAAAGAGAAGGCAAA+CGG 0.539093 5.2:+83748890 None:intergenic
CCTCAAAATCAAACGCATGA+AGG 0.539206 5.2:-83745782 MS.gene65373:CDS
CAACTCTTATGTCATTCCGA+AGG 0.542501 5.2:+83747392 None:intergenic
AACATGGCTAAAATTCTAGC+TGG 0.543477 5.2:-83746179 MS.gene65373:CDS
GTTGACACCGGCATTACTAC+AGG 0.543830 5.2:+83748504 None:intergenic
GCAGATAATTGATGCTGTTG+TGG 0.547639 5.2:-83747663 MS.gene65373:intron
AAACGGAGATGGCGGAAATG+AGG 0.548034 5.2:+83748907 None:intergenic
GAGAGCTCTGAGGGGTGCTA+GGG 0.550613 5.2:+83746340 None:intergenic
TGGCGGAAATGAGGAGGCGA+TGG 0.552680 5.2:+83748916 None:intergenic
GCGATTAACAAGTATCGATC+CGG 0.554337 5.2:+83748346 None:intergenic
AGAAAGCAGGTTCAGCTCAA+AGG 0.555946 5.2:-83745674 MS.gene65373:CDS
AACAAACAACAATGGGAACA+TGG 0.556243 5.2:-83746195 MS.gene65373:CDS
CGAGTTCGAACTTTCCAGGT+AGG 0.556717 5.2:-83745920 MS.gene65373:CDS
AACTCTTATGTCATTCCGAA+GGG 0.557571 5.2:+83747393 None:intergenic
AAATGGGGCCAATTCCTCCT+TGG 0.558531 5.2:+83746283 None:intergenic
TTTCCGTTAGGGCGGATGGT+AGG 0.563392 5.2:+83746001 None:intergenic
AATCCGTAAATTTGAGAGCA+TGG 0.564183 5.2:+83746985 None:intergenic
TGGGAAATTTGTTGGTTGAG+CGG 0.564822 5.2:-83746950 MS.gene65373:CDS
TTGTTAATCGCAACCAGTGG+AGG 0.567512 5.2:-83748334 MS.gene65373:CDS
AATTGAAACGCATTTGTATG+TGG 0.568739 5.2:-83746393 MS.gene65373:CDS
TTGGTCAGTACCCATTAGAA+GGG 0.570383 5.2:+83745631 None:intergenic
AAACCTACCATCCGCCCTAA+CGG 0.572836 5.2:-83746004 MS.gene65373:CDS
TATTCTTGTTCATGAATAAG+GGG 0.577205 5.2:+83745969 None:intergenic
GGCGAAGGAAACTGGCAAGA+AGG 0.588540 5.2:+83748949 None:intergenic
TCTTGCATATGTCAAAACTC+AGG 0.590986 5.2:-83745836 MS.gene65373:CDS
GATGGATGAGACGACGGCGA+AGG 0.593369 5.2:+83748934 None:intergenic
GAGAAGGCAAACGGAGATGG+CGG 0.595462 5.2:+83748899 None:intergenic
CCACTTACCCCAGAGGAAGT+TGG 0.600004 5.2:-83746443 MS.gene65373:CDS
GCATGATGAAAAGAAAGAGA+AGG 0.602057 5.2:+83748883 None:intergenic
GCTTAAATCAACAGAGAACA+GGG 0.602960 5.2:-83748312 MS.gene65373:CDS
AAGAGCATTTACAATAAAAG+GGG 0.610292 5.2:+83748842 None:intergenic
AATGAGTTGAGGTCAGGCAG+TGG 0.610497 5.2:-83745881 MS.gene65373:CDS
TCTCCTTTGAATGGAAGTTG+GGG 0.613782 5.2:+83746315 None:intergenic
CTTGCATATGTCAAAACTCA+GGG 0.615011 5.2:-83745835 MS.gene65373:CDS
CGGAGATGGCGGAAATGAGG+AGG 0.616366 5.2:+83748910 None:intergenic
GCATTTACAATAAAAGGGGA+AGG 0.618091 5.2:+83748846 None:intergenic
AAAGGAGACCTTAGCTACCA+AGG 0.620774 5.2:-83746300 MS.gene65373:CDS
GACAAGTCCAGAAGACTCAT+CGG 0.622144 5.2:-83748975 MS.gene65373:CDS
AACTTGGTGAAATTAGGAAG+CGG 0.626657 5.2:-83746608 MS.gene65373:CDS
TGGAGAAATATATGGAGGCG+AGG 0.630114 5.2:-83746366 MS.gene65373:CDS
ATGTCAAAACTCAGGGTCGG+AGG 0.632035 5.2:-83745828 MS.gene65373:CDS
TACTTGTTAATCGCAACCAG+TGG 0.633957 5.2:-83748337 MS.gene65373:CDS
TTTACTTGCAAAGTTGACAC+CGG 0.634644 5.2:+83748492 None:intergenic
GACAGAAAACCAGCCTGCTG+AGG 0.636575 5.2:-83745741 MS.gene65373:CDS
TGGGAGAGCCTAATGAGTTG+AGG 0.637626 5.2:-83745892 MS.gene65373:CDS
TGGTCAGTACCCATTAGAAG+GGG 0.642645 5.2:+83745632 None:intergenic
TTGGTGAAATTAGGAAGCGG+TGG 0.644603 5.2:-83746605 MS.gene65373:CDS
AGAAAGTAAGAAGACATGGA+AGG 0.646390 5.2:-83746469 MS.gene65373:CDS
CATATGTCAAAACTCAGGGT+CGG 0.649933 5.2:-83745831 MS.gene65373:CDS
CTGCTGAGGAAGTACGAGAG+TGG 0.653675 5.2:-83745727 MS.gene65373:CDS
TCTACAAAAGCTGAGGTGCA+GGG 0.657185 5.2:-83747015 MS.gene65373:CDS
TGAACAAGAATAACATGACA+TGG 0.657343 5.2:-83745958 MS.gene65373:CDS
AGTTGGGGAAGAGAGCTCTG+AGG 0.685910 5.2:+83746330 None:intergenic
GGAGGCGATGGATGAGACGA+CGG 0.693598 5.2:+83748928 None:intergenic
GCTTCTGGAGAAATATATGG+AGG 0.698383 5.2:-83746371 MS.gene65373:CDS
GCGACCCTCGTAGCATTTGG+GGG 0.700748 5.2:+83747300 None:intergenic
TGAAGAGAAAGACACAAATG+GGG 0.704290 5.2:+83746268 None:intergenic
GGTCAGTACCCATTAGAAGG+GGG 0.707285 5.2:+83745633 None:intergenic
ATGATGATCTACAAAAGCTG+AGG 0.712332 5.2:-83747022 MS.gene65373:CDS
CATAAGACCAACTTCCTCTG+GGG 0.724014 5.2:+83746436 None:intergenic
CTTAAATCAACAGAGAACAG+GGG 0.728232 5.2:-83748311 MS.gene65373:intron
AAGGTGTCCACTTACCCCAG+AGG 0.746305 5.2:-83746450 MS.gene65373:CDS
TTGGGGAAGAGAGCTCTGAG+GGG 0.774845 5.2:+83746332 None:intergenic
GGAGACCTTAGCTACCAAGG+AGG 0.785211 5.2:-83746297 MS.gene65373:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAGTTATAATTATCAAA+TGG + chr5.2:83747752-83747771 None:intergenic 10.0%
!! TATATTATAAATGAAATAGA+AGG + chr5.2:83745830-83745849 None:intergenic 10.0%
!!! AAAATGTAAATTATTGTTTT+AGG + chr5.2:83747828-83747847 None:intergenic 10.0%
!! AAAAATTATAGATTGTTTGT+GGG - chr5.2:83746786-83746805 MS.gene65373:intron 15.0%
!! AAATACTATTATATATAGAG+TGG - chr5.2:83746549-83746568 MS.gene65373:intron 15.0%
!! AATCAAAAGCAATTAAATAT+AGG + chr5.2:83746208-83746227 None:intergenic 15.0%
!! CAAATATTACATACAAATTA+AGG + chr5.2:83747155-83747174 None:intergenic 15.0%
!! TTAAATGTTATACAATCATA+TGG + chr5.2:83747526-83747545 None:intergenic 15.0%
!!! ATCACATATAATATTTTCTT+TGG - chr5.2:83747103-83747122 MS.gene65373:intron 15.0%
!!! ATGAAAATAATTTTGATGAA+AGG + chr5.2:83747412-83747431 None:intergenic 15.0%
!! AAAAAATACAGATTGTTTGT+GGG - chr5.2:83746940-83746959 MS.gene65373:CDS 20.0%
!! AAAGAAAATGAAATTGAAGT+TGG + chr5.2:83747875-83747894 None:intergenic 20.0%
!! ACAATTAATGAGATGAAAAA+AGG + chr5.2:83746181-83746200 None:intergenic 20.0%
!! CATAAAAAAAAAAGTTGAGA+AGG + chr5.2:83745987-83746006 None:intergenic 20.0%
!! GAAAAATTATAGATTGTTTG+TGG - chr5.2:83746785-83746804 MS.gene65373:intron 20.0%
!! TTATTCTTGTTCATGAATAA+GGG + chr5.2:83748662-83748681 None:intergenic 20.0%
!!! TTGTTTTTTCTGTTTCATAT+AGG - chr5.2:83747932-83747951 MS.gene65373:intron 20.0%
!!! TTTTGTTTATTGGAATTTGA+AGG - chr5.2:83746040-83746059 MS.gene65373:intron 20.0%
!!! TTTTTGTAACAAACAACAAT+GGG - chr5.2:83748425-83748444 MS.gene65373:intron 20.0%
! AACATTTAATTGCATTACAC+AGG - chr5.2:83747537-83747556 MS.gene65373:intron 25.0%
! AAGGAGGATAACTTATTAAT+CGG - chr5.2:83746435-83746454 MS.gene65373:CDS 25.0%
! AATCTCTACCCAATAATTAT+AGG - chr5.2:83746605-83746624 MS.gene65373:CDS 25.0%
! ACAATTATCACATTGAACTT+CGG + chr5.2:83747899-83747918 None:intergenic 25.0%
! CTTAATTTATTCCAAGTAGT+TGG - chr5.2:83748525-83748544 MS.gene65373:CDS 25.0%
! GAAAAAATACAGATTGTTTG+TGG - chr5.2:83746939-83746958 MS.gene65373:CDS 25.0%
! GAATGATATATTACGTTATG+TGG - chr5.2:83746740-83746759 MS.gene65373:intron 25.0%
! GTCTGAATTAGATTATGATT+TGG - chr5.2:83748922-83748941 MS.gene65373:CDS 25.0%
! GTTATTCTTGTTCATGAATA+AGG + chr5.2:83748663-83748682 None:intergenic 25.0%
! TATAACTAAATATTTGTCCC+TGG - chr5.2:83747761-83747780 MS.gene65373:intron 25.0%
! TATTCTTGTTCATGAATAAG+GGG + chr5.2:83748661-83748680 None:intergenic 25.0%
! TGTAACTTTGATTGTATTTC+AGG - chr5.2:83745887-83745906 MS.gene65373:CDS 25.0%
! TGTATTTCAGGAAAATGATT+TGG - chr5.2:83745899-83745918 MS.gene65373:CDS 25.0%
!! GTTTTTGTAACAAACAACAA+TGG - chr5.2:83748424-83748443 MS.gene65373:intron 25.0%
!! TGGTCATTTACAAAAAGTAT+AGG - chr5.2:83746578-83746597 MS.gene65373:intron 25.0%
!!! AAGAGCATTTACAATAAAAG+GGG + chr5.2:83745788-83745807 None:intergenic 25.0%
!!! ATACTTTTTGTAAATGACCA+CGG + chr5.2:83746578-83746597 None:intergenic 25.0%
!!! GAAGAGCATTTACAATAAAA+GGG + chr5.2:83745789-83745808 None:intergenic 25.0%
!!! TCCTAATTGTGTTTTTTTAG+CGG + chr5.2:83746678-83746697 None:intergenic 25.0%
!!! TCGATCTTCTTTTTGTTTAT+TGG - chr5.2:83746030-83746049 MS.gene65373:intron 25.0%
!!! TGAAGAGCATTTACAATAAA+AGG + chr5.2:83745790-83745809 None:intergenic 25.0%
AAAGATGTATGATCCAATTC+TGG + chr5.2:83747022-83747041 None:intergenic 30.0%
AAATTTACGGATTCGATTCA+GGG - chr5.2:83747652-83747671 MS.gene65373:CDS 30.0%
AACAGAAAAAACAAGCAGTT+TGG + chr5.2:83747926-83747945 None:intergenic 30.0%
AAGAGTAGCATTCAAAAGAT+AGG + chr5.2:83746993-83747012 None:intergenic 30.0%
AAGGGATGTTATAAACCAAT+CGG + chr5.2:83747219-83747238 None:intergenic 30.0%
AATGAGATGAAAAAAGGACT+GGG + chr5.2:83746175-83746194 None:intergenic 30.0%
AATTGAAACGCATTTGTATG+TGG - chr5.2:83748234-83748253 MS.gene65373:intron 30.0%
AATTGGATCATACATCTTTC+TGG - chr5.2:83747023-83747042 MS.gene65373:CDS 30.0%
ACAAATTTCTCTCCCATATT+TGG + chr5.2:83747272-83747291 None:intergenic 30.0%
ATTATAGGTCTGCAACAAAT+TGG - chr5.2:83746620-83746639 MS.gene65373:CDS 30.0%
ATTTCACTTCTATCATATCC+AGG - chr5.2:83747069-83747088 MS.gene65373:CDS 30.0%
CTAGATCAAATTTGATGAAG+TGG - chr5.2:83746897-83746916 MS.gene65373:intron 30.0%
GAAAAGGAAAGAAAAGAACT+TGG - chr5.2:83748003-83748022 MS.gene65373:intron 30.0%
GATTAATAAGTTATCCTCCT+TGG + chr5.2:83746436-83746455 None:intergenic 30.0%
GGTGGAAAAATTTACATGTA+AGG - chr5.2:83748040-83748059 MS.gene65373:intron 30.0%
GTTGCAGACCTATAATTATT+GGG + chr5.2:83746616-83746635 None:intergenic 30.0%
TAATGAGATGAAAAAAGGAC+TGG + chr5.2:83746176-83746195 None:intergenic 30.0%
TCCGCTAAAAAAACACAATT+AGG - chr5.2:83746674-83746693 MS.gene65373:CDS 30.0%
TCGGCTAAAATAACTCAATT+AGG - chr5.2:83746869-83746888 MS.gene65373:intron 30.0%
TCTAATTAAAGGTCCCTAAT+CGG - chr5.2:83747384-83747403 MS.gene65373:CDS 30.0%
TGAACAAGAATAACATGACA+TGG - chr5.2:83748669-83748688 MS.gene65373:intron 30.0%
TGATAATTAATGTGACTGAC+AGG - chr5.2:83748123-83748142 MS.gene65373:intron 30.0%
TGCTACAATAACTAGTATGT+TGG + chr5.2:83747490-83747509 None:intergenic 30.0%
TGTTGCAGACCTATAATTAT+TGG + chr5.2:83746617-83746636 None:intergenic 30.0%
TGTTTCATATAGGTTTGAAC+CGG - chr5.2:83747942-83747961 MS.gene65373:intron 30.0%
TTTGGACAATTATAGAAAGC+AGG - chr5.2:83748940-83748959 MS.gene65373:CDS 30.0%
! AGAAGGTAAGCTAGTTTATT+AGG - chr5.2:83748472-83748491 MS.gene65373:intron 30.0%
! ATTGGTTTATAACATCCCTT+CGG - chr5.2:83747219-83747238 MS.gene65373:intron 30.0%
! GAAAAGAACTTGGTGAAATT+AGG - chr5.2:83748013-83748032 MS.gene65373:intron 30.0%
! GAAGGTAAGCTAGTTTATTA+GGG - chr5.2:83748473-83748492 MS.gene65373:intron 30.0%
!! CAAATTTTGTATGTGCCATT+CGG - chr5.2:83746850-83746869 MS.gene65373:intron 30.0%
!! CATATTTCTAACTTTTGCAG+AGG - chr5.2:83746241-83746260 MS.gene65373:CDS 30.0%
!! TACTCTTGTTATTCCAGAAT+TGG - chr5.2:83747006-83747025 MS.gene65373:CDS 30.0%
!!! ATTGAGTTATTTTAGCCGAA+TGG + chr5.2:83746868-83746887 None:intergenic 30.0%
!!! ATTGTGTTTTTTTAGCGGAA+TGG + chr5.2:83746673-83746692 None:intergenic 30.0%
!!! TTGTGTTTTTTTAGCGGAAT+GGG + chr5.2:83746672-83746691 None:intergenic 30.0%
AACAAACAACAATGGGAACA+TGG - chr5.2:83748432-83748451 MS.gene65373:intron 35.0%
AACATGGCTAAAATTCTAGC+TGG - chr5.2:83748448-83748467 MS.gene65373:intron 35.0%
AACTCTTATGTCATTCCGAA+GGG + chr5.2:83747237-83747256 None:intergenic 35.0%
AAGGCAATAAGGTAGAAAAC+AGG - chr5.2:83748864-83748883 MS.gene65373:CDS 35.0%
AATCCGTAAATTTGAGAGCA+TGG + chr5.2:83747645-83747664 None:intergenic 35.0%
AGAAAGTAAGAAGACATGGA+AGG - chr5.2:83748158-83748177 MS.gene65373:intron 35.0%
ATGATGATCTACAAAAGCTG+AGG - chr5.2:83747605-83747624 MS.gene65373:CDS 35.0%
ATGTGATTTAAGAGACATCC+TGG + chr5.2:83747090-83747109 None:intergenic 35.0%
ATTCAGACTTTCTCTGTTGT+AGG - chr5.2:83748585-83748604 MS.gene65373:intron 35.0%
ATTGCATTACACAGGTTGTT+CGG - chr5.2:83747545-83747564 MS.gene65373:intron 35.0%
CAAATTGATACGAAAGCTCT+AGG - chr5.2:83747849-83747868 MS.gene65373:intron 35.0%
CAAATTTACGGATTCGATTC+AGG - chr5.2:83747651-83747670 MS.gene65373:CDS 35.0%
CCAAATAGAAACCAACTACT+TGG + chr5.2:83748539-83748558 None:intergenic 35.0%
CTACCATGCTCTCAAATTTA+CGG - chr5.2:83747639-83747658 MS.gene65373:CDS 35.0%
CTTAAATCAACAGAGAACAG+GGG - chr5.2:83746316-83746335 MS.gene65373:CDS 35.0%
CTTGCATATGTCAAAACTCA+GGG - chr5.2:83748792-83748811 MS.gene65373:intron 35.0%
GAAAAGAAAGAGAAGGCAAA+CGG + chr5.2:83745740-83745759 None:intergenic 35.0%
GATTGAATTTGACGAATGAG+TGG - chr5.2:83746705-83746724 MS.gene65373:intron 35.0%
GCATGATGAAAAGAAAGAGA+AGG + chr5.2:83745747-83745766 None:intergenic 35.0%
GCATTTACAATAAAAGGGGA+AGG + chr5.2:83745784-83745803 None:intergenic 35.0%
GCGTGTAAGTGTCTAATTAA+AGG - chr5.2:83747373-83747392 MS.gene65373:CDS 35.0%
GCTTAAATCAACAGAGAACA+GGG - chr5.2:83746315-83746334 MS.gene65373:CDS 35.0%
GGATACCTAACTTACAATGT+TGG + chr5.2:83747469-83747488 None:intergenic 35.0%
GTAAAGCAGCTACCAAATAT+GGG - chr5.2:83747257-83747276 MS.gene65373:intron 35.0%
GTCTGAATATCAGTATGTGT+AGG + chr5.2:83748573-83748592 None:intergenic 35.0%
GTGAAGAGAAAGACACAAAT+GGG + chr5.2:83748363-83748382 None:intergenic 35.0%
TAGAAGGTAGTTGAAGTAGA+AGG + chr5.2:83745814-83745833 None:intergenic 35.0%
TCTTGCATATGTCAAAACTC+AGG - chr5.2:83748791-83748810 MS.gene65373:intron 35.0%
TGAAGAGAAAGACACAAATG+GGG + chr5.2:83748362-83748381 None:intergenic 35.0%
TGAAGTGGATGCAATATTTC+TGG - chr5.2:83746912-83746931 MS.gene65373:CDS 35.0%
TGAGAAGGTTGTTTGATTAC+CGG + chr5.2:83745972-83745991 None:intergenic 35.0%
TGGTTCCAACATTGTAAGTT+AGG - chr5.2:83747461-83747480 MS.gene65373:intron 35.0%
TGTAAAGCAGCTACCAAATA+TGG - chr5.2:83747256-83747275 MS.gene65373:intron 35.0%
TGTGGATGATGCAATATTTC+TGG - chr5.2:83746758-83746777 MS.gene65373:intron 35.0%
TTCTTTCTCTTCACTGATGA+TGG + chr5.2:83745616-83745635 None:intergenic 35.0%
TTGATGAAAGGTTCCGATTA+GGG + chr5.2:83747400-83747419 None:intergenic 35.0%
TTGATTACCGGAATGTGAAA+TGG + chr5.2:83745960-83745979 None:intergenic 35.0%
TTTACTTGCAAAGTTGACAC+CGG + chr5.2:83746138-83746157 None:intergenic 35.0%
TTTGATGAAAGGTTCCGATT+AGG + chr5.2:83747401-83747420 None:intergenic 35.0%
TTTGTGGGTGCAAGAATTAT+AGG - chr5.2:83746801-83746820 MS.gene65373:intron 35.0%
! AACTTGGTGAAATTAGGAAG+CGG - chr5.2:83748019-83748038 MS.gene65373:intron 35.0%
! CCAAGTAGTTGGTTTCTATT+TGG - chr5.2:83748536-83748555 MS.gene65373:CDS 35.0%
! CTAAAATTCTAGCTGGAAGA+AGG - chr5.2:83748455-83748474 MS.gene65373:intron 35.0%
! CTCAATTATTTGTTGCCGAT+TGG - chr5.2:83747201-83747220 MS.gene65373:intron 35.0%
! TTTTGACATATGCAAGACGT+TGG + chr5.2:83748788-83748807 None:intergenic 35.0%
!! ATGCGTTTGATTTTGAGGAT+TGG + chr5.2:83748843-83748862 None:intergenic 35.0%
!!! GGTTCAACTTTTTTCCGTTA+GGG + chr5.2:83748640-83748659 None:intergenic 35.0%
AATGGCATATCAAAGGAACC+AGG + chr5.2:83747782-83747801 None:intergenic 40.0%
AGAATACTTGCACTTCAGCT+TGG + chr5.2:83749018-83749037 None:intergenic 40.0%
AGGCAAATGAAGCTACTTAC+AGG - chr5.2:83746060-83746079 MS.gene65373:intron 40.0%
ATGGCATATCAAAGGAACCA+GGG + chr5.2:83747781-83747800 None:intergenic 40.0%
CAACTCTTATGTCATTCCGA+AGG + chr5.2:83747238-83747257 None:intergenic 40.0%
CATATGTCAAAACTCAGGGT+CGG - chr5.2:83748796-83748815 MS.gene65373:intron 40.0%
CCATGTCTTCTTACTTTCTC+AGG + chr5.2:83748157-83748176 None:intergenic 40.0%
CCTCAAAATCAAACGCATGA+AGG - chr5.2:83748845-83748864 MS.gene65373:intron 40.0%
CCTGAGAAAGTAAGAAGACA+TGG - chr5.2:83748154-83748173 MS.gene65373:intron 40.0%
CGTGAAGAGAAAGACACAAA+TGG + chr5.2:83748364-83748383 None:intergenic 40.0%
CTACAGACTTTCTAATCTGC+TGG - chr5.2:83747582-83747601 MS.gene65373:intron 40.0%
CTACTTACAGGCATGATCTA+TGG - chr5.2:83746072-83746091 MS.gene65373:intron 40.0%
GCAGATAATTGATGCTGTTG+TGG - chr5.2:83746964-83746983 MS.gene65373:CDS 40.0%
GCTGAAGAATGGCATATCAA+AGG + chr5.2:83747789-83747808 None:intergenic 40.0%
GGCTAATACCAAAACTCATC+CGG - chr5.2:83746262-83746281 MS.gene65373:CDS 40.0%
GGCTTAAATCAACAGAGAAC+AGG - chr5.2:83746314-83746333 MS.gene65373:CDS 40.0%
GTCTCCTTTGAATGGAAGTT+GGG + chr5.2:83748316-83748335 None:intergenic 40.0%
GTTTCATCGCGCTACATTTA+AGG - chr5.2:83747719-83747738 MS.gene65373:intron 40.0%
TAGCTAAGGTCTCCTTTGAA+TGG + chr5.2:83748324-83748343 None:intergenic 40.0%
TCAAACGCATGAAGGCAATA+AGG - chr5.2:83748853-83748872 MS.gene65373:intron 40.0%
TCAGGGAATGGGAAATTTGT+TGG - chr5.2:83747669-83747688 MS.gene65373:intron 40.0%
TCTCCTTTGAATGGAAGTTG+GGG + chr5.2:83748315-83748334 None:intergenic 40.0%
TGAATGTATAGAGCAGTCCA+AGG - chr5.2:83746416-83746435 MS.gene65373:CDS 40.0%
TTGGTCAGTACCCATTAGAA+GGG + chr5.2:83748999-83749018 None:intergenic 40.0%
! CATCTTTCTGGAAAGACAAC+AGG - chr5.2:83747035-83747054 MS.gene65373:CDS 40.0%
! GCATTCTCTGGTTGCTATTA+TGG - chr5.2:83747973-83747992 MS.gene65373:intron 40.0%
! GCTTCTGGAGAAATATATGG+AGG - chr5.2:83748256-83748275 MS.gene65373:intron 40.0%
! GTGGCTTCTGGAGAAATATA+TGG - chr5.2:83748253-83748272 MS.gene65373:intron 40.0%
! TACTTGTTAATCGCAACCAG+TGG - chr5.2:83746290-83746309 MS.gene65373:CDS 40.0%
! TCTCTGGTTGCTATTATGGT+GGG - chr5.2:83747977-83747996 MS.gene65373:intron 40.0%
! TTCTCTGGTTGCTATTATGG+TGG - chr5.2:83747976-83747995 MS.gene65373:intron 40.0%
!! ATGCTTAGAGCACTAGGTTT+TGG - chr5.2:83748211-83748230 MS.gene65373:intron 40.0%
!! CCTTCATGCGTTTGATTTTG+AGG + chr5.2:83748848-83748867 None:intergenic 40.0%
!! GCGATTAACAAGTATCGATC+CGG + chr5.2:83746284-83746303 None:intergenic 40.0%
!! TGGGAAATTTGTTGGTTGAG+CGG - chr5.2:83747677-83747696 MS.gene65373:intron 40.0%
!!! GGGTTCAACTTTTTTCCGTT+AGG + chr5.2:83748641-83748660 None:intergenic 40.0%
!!! TCAACTTTTTTCCGTTAGGG+CGG + chr5.2:83748637-83748656 None:intergenic 40.0%
AAAGAGAAGGCAAACGGAGA+TGG + chr5.2:83745734-83745753 None:intergenic 45.0%
AAAGGAGACCTTAGCTACCA+AGG - chr5.2:83748327-83748346 MS.gene65373:CDS 45.0%
AACTTTCCAGGTAGGCTTCA+TGG - chr5.2:83748715-83748734 MS.gene65373:CDS 45.0%
ACGCATTTGTATGTGGCTTC+TGG - chr5.2:83748241-83748260 MS.gene65373:intron 45.0%
ACGGATTCGATTCAGGGAAT+GGG - chr5.2:83747658-83747677 MS.gene65373:CDS 45.0%
ACTTTCCAGGTAGGCTTCAT+GGG - chr5.2:83748716-83748735 MS.gene65373:CDS 45.0%
AGAAAGCAGGTTCAGCTCAA+AGG - chr5.2:83748953-83748972 MS.gene65373:CDS 45.0%
AGCATAAGACCAACTTCCTC+TGG + chr5.2:83748196-83748215 None:intergenic 45.0%
ATCTACAAAAGCTGAGGTGC+AGG - chr5.2:83747611-83747630 MS.gene65373:CDS 45.0%
ATCTCGAGTTCGAACTTTCC+AGG - chr5.2:83748703-83748722 MS.gene65373:CDS 45.0%
ATGTATAGAGCAGTCCAAGG+AGG - chr5.2:83746419-83746438 MS.gene65373:CDS 45.0%
CAGACTTTCTCTGTTGTAGG+AGG - chr5.2:83748588-83748607 MS.gene65373:intron 45.0%
CATAAGACCAACTTCCTCTG+GGG + chr5.2:83748194-83748213 None:intergenic 45.0%
CATATACAGTGCGTGTTCCA+AGG - chr5.2:83747297-83747316 MS.gene65373:CDS 45.0%
CCAGAGAATGCAAGCATATC+CGG + chr5.2:83747964-83747983 None:intergenic 45.0%
CTTAGCTACCAAGGAGGAAT+TGG - chr5.2:83748336-83748355 MS.gene65373:CDS 45.0%
CTTCCCCAACTTCCATTCAA+AGG - chr5.2:83748309-83748328 MS.gene65373:intron 45.0%
CTTGGAACACGCACTGTATA+TGG + chr5.2:83747299-83747318 None:intergenic 45.0%
CTTGGTCAGTACCCATTAGA+AGG + chr5.2:83749000-83749019 None:intergenic 45.0%
GAAAAAAGGACTGGGAGTCT+AGG + chr5.2:83746167-83746186 None:intergenic 45.0%
GACAAGTCCAGAAGACTCAT+CGG - chr5.2:83745652-83745671 MS.gene65373:CDS 45.0%
GCAATCGCCATTTCACATTC+CGG - chr5.2:83745950-83745969 MS.gene65373:CDS 45.0%
GCATAAGACCAACTTCCTCT+GGG + chr5.2:83748195-83748214 None:intergenic 45.0%
GCTGATCACATGCTGAAGAA+TGG + chr5.2:83747800-83747819 None:intergenic 45.0%
GGTCTCCTTTGAATGGAAGT+TGG + chr5.2:83748317-83748336 None:intergenic 45.0%
TACGGATTCGATTCAGGGAA+TGG - chr5.2:83747657-83747676 MS.gene65373:CDS 45.0%
TATATATAGAGTGGCGACCG+TGG - chr5.2:83746558-83746577 MS.gene65373:intron 45.0%
TCTACAAAAGCTGAGGTGCA+GGG - chr5.2:83747612-83747631 MS.gene65373:CDS 45.0%
TGGAGAAATATATGGAGGCG+AGG - chr5.2:83748261-83748280 MS.gene65373:intron 45.0%
TGGTCAGTACCCATTAGAAG+GGG + chr5.2:83748998-83749017 None:intergenic 45.0%
TGTGTCTTTCTCTTCACGCA+TGG - chr5.2:83748366-83748385 MS.gene65373:CDS 45.0%
TTCTCTGCCTGTAGTAATGC+CGG - chr5.2:83746116-83746135 MS.gene65373:intron 45.0%
TTGGTGAAATTAGGAAGCGG+TGG - chr5.2:83748022-83748041 MS.gene65373:intron 45.0%
TTGTTAATCGCAACCAGTGG+AGG - chr5.2:83746293-83746312 MS.gene65373:CDS 45.0%
! CAATTCCTCCTTGGTAGCTA+AGG + chr5.2:83748338-83748357 None:intergenic 45.0%
! CTATTATGGTGGGGGAGAAA+AGG - chr5.2:83747987-83748006 MS.gene65373:intron 45.0%
! CTCTGGTTGCTATTATGGTG+GGG - chr5.2:83747978-83747997 MS.gene65373:intron 45.0%
! GGTCTTATGCTTAGAGCACT+AGG - chr5.2:83748205-83748224 MS.gene65373:intron 45.0%
! TCTGGTTGCTATTATGGTGG+GGG - chr5.2:83747979-83747998 MS.gene65373:intron 45.0%
!! CTCTGTTGTAGGAGGTACTT+TGG - chr5.2:83748596-83748615 MS.gene65373:intron 45.0%
!! CTGTTGATTTAAGCCTCCAC+TGG + chr5.2:83746309-83746328 None:intergenic 45.0%
!! GTATCGATCCGGATGAGTTT+TGG + chr5.2:83746273-83746292 None:intergenic 45.0%
!! AAAATTTATTAATAAAATTG+AGG + chr5.2:83747440-83747459 None:intergenic 5.0%
!! AAATTTATTAATAAAATTGA+GGG + chr5.2:83747439-83747458 None:intergenic 5.0%
!!! AATTTTATTAATAAATTTTG+TGG - chr5.2:83747441-83747460 MS.gene65373:intron 5.0%
AAACCTACCATCCGCCCTAA+CGG - chr5.2:83748623-83748642 MS.gene65373:intron 50.0%
AAATGGGGCCAATTCCTCCT+TGG + chr5.2:83748347-83748366 None:intergenic 50.0%
AAGGAGTCCGATGAGTCTTC+TGG + chr5.2:83745662-83745681 None:intergenic 50.0%
AGGGTCGGAGGTAAAAACTG+AGG - chr5.2:83748811-83748830 MS.gene65373:intron 50.0%
ATGTCAAAACTCAGGGTCGG+AGG - chr5.2:83748799-83748818 MS.gene65373:intron 50.0%
CCGGATATGCTTGCATTCTC+TGG - chr5.2:83747961-83747980 MS.gene65373:intron 50.0%
CGAGTTCGAACTTTCCAGGT+AGG - chr5.2:83748707-83748726 MS.gene65373:CDS 50.0%
CTGCCTGACCTCAACTCATT+AGG + chr5.2:83748746-83748765 None:intergenic 50.0%
GGGTCGGAGGTAAAAACTGA+GGG - chr5.2:83748812-83748831 MS.gene65373:intron 50.0%
GGTCAGTACCCATTAGAAGG+GGG + chr5.2:83748997-83749016 None:intergenic 50.0%
GTTGACACCGGCATTACTAC+AGG + chr5.2:83746126-83746145 None:intergenic 50.0%
TGGGAGAGCCTAATGAGTTG+AGG - chr5.2:83748735-83748754 MS.gene65373:intron 50.0%
! AATGAGTTGAGGTCAGGCAG+TGG - chr5.2:83748746-83748765 MS.gene65373:intron 50.0%
! CATTTGGGGGTGCATTTCCT+TGG + chr5.2:83747317-83747336 None:intergenic 50.0%
! GAGCCTAATGAGTTGAGGTC+AGG - chr5.2:83748740-83748759 MS.gene65373:intron 50.0%
!! AAACGGAGATGGCGGAAATG+AGG + chr5.2:83745723-83745742 None:intergenic 50.0%
!!! CTTTTTTCCGTTAGGGCGGA+TGG + chr5.2:83748633-83748652 None:intergenic 50.0%
AACGCGACCCTCGTAGCATT+TGG + chr5.2:83747333-83747352 None:intergenic 55.0%
ACACGCTTCTTGACCAGCAC+TGG + chr5.2:83747359-83747378 None:intergenic 55.0%
ACGCGACCCTCGTAGCATTT+GGG + chr5.2:83747332-83747351 None:intergenic 55.0%
AGTTGGGGAAGAGAGCTCTG+AGG + chr5.2:83748300-83748319 None:intergenic 55.0%
ATGCACCCCCAAATGCTACG+AGG - chr5.2:83747322-83747341 MS.gene65373:CDS 55.0%
CACTCTCGTACTTCCTCAGC+AGG + chr5.2:83748902-83748921 None:intergenic 55.0%
CCACTTACCCCAGAGGAAGT+TGG - chr5.2:83748184-83748203 MS.gene65373:intron 55.0%
GACAGAAAACCAGCCTGCTG+AGG - chr5.2:83748886-83748905 MS.gene65373:CDS 55.0%
GAGAAGGCAAACGGAGATGG+CGG + chr5.2:83745731-83745750 None:intergenic 55.0%
GAGTCGCTCCCCCTTCTAAT+GGG - chr5.2:83748986-83749005 MS.gene65373:CDS 55.0%
GGAGACCTTAGCTACCAAGG+AGG - chr5.2:83748330-83748349 MS.gene65373:CDS 55.0%
GGCGAAGGAAACTGGCAAGA+AGG + chr5.2:83745681-83745700 None:intergenic 55.0%
TGAGTCGCTCCCCCTTCTAA+TGG - chr5.2:83748985-83749004 MS.gene65373:CDS 55.0%
TGCACCCCCAAATGCTACGA+GGG - chr5.2:83747323-83747342 MS.gene65373:CDS 55.0%
TTTCCGTTAGGGCGGATGGT+AGG + chr5.2:83748629-83748648 None:intergenic 55.0%
! AAGGTGTCCACTTACCCCAG+AGG - chr5.2:83748177-83748196 MS.gene65373:intron 55.0%
! CTGCTGAGGAAGTACGAGAG+TGG - chr5.2:83748900-83748919 MS.gene65373:CDS 55.0%
! GTTGGGGAAGAGAGCTCTGA+GGG + chr5.2:83748299-83748318 None:intergenic 55.0%
!! CCAACTTCCTCTGGGGTAAG+TGG + chr5.2:83748187-83748206 None:intergenic 55.0%
!! TTGGGGAAGAGAGCTCTGAG+GGG + chr5.2:83748298-83748317 None:intergenic 55.0%
CGCGACCCTCGTAGCATTTG+GGG + chr5.2:83747331-83747350 None:intergenic 60.0%
CTCGTACTTCCTCAGCAGGC+TGG + chr5.2:83748898-83748917 None:intergenic 60.0%
GAGACGACGGCGAAGGAAAC+TGG + chr5.2:83745689-83745708 None:intergenic 60.0%
GATGGATGAGACGACGGCGA+AGG + chr5.2:83745696-83745715 None:intergenic 60.0%
GCGACCCTCGTAGCATTTGG+GGG + chr5.2:83747330-83747349 None:intergenic 60.0%
GCTCTCCCATGAAGCCTACC+TGG + chr5.2:83748724-83748743 None:intergenic 60.0%
GGAGGCGATGGATGAGACGA+CGG + chr5.2:83745702-83745721 None:intergenic 60.0%
TGGCGGAAATGAGGAGGCGA+TGG + chr5.2:83745714-83745733 None:intergenic 60.0%
! GGGTCGCGTTTTACCAGTGC+TGG - chr5.2:83747343-83747362 MS.gene65373:CDS 60.0%
!! AGAGAGCTCTGAGGGGTGCT+AGG + chr5.2:83748291-83748310 None:intergenic 60.0%
!! CGGAGATGGCGGAAATGAGG+AGG + chr5.2:83745720-83745739 None:intergenic 60.0%
!! GAGAGCTCTGAGGGGTGCTA+GGG + chr5.2:83748290-83748309 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 83745601 83749048 83745601 ID=MS.gene65373
chr5.2 mRNA 83745601 83749048 83745601 ID=MS.gene65373.t1;Parent=MS.gene65373
chr5.2 exon 83748854 83749048 83748854 ID=MS.gene65373.t1.exon1;Parent=MS.gene65373.t1
chr5.2 CDS 83748854 83749048 83748854 ID=cds.MS.gene65373.t1;Parent=MS.gene65373.t1
chr5.2 exon 83748678 83748740 83748678 ID=MS.gene65373.t1.exon2;Parent=MS.gene65373.t1
chr5.2 CDS 83748678 83748740 83748678 ID=cds.MS.gene65373.t1;Parent=MS.gene65373.t1
chr5.2 exon 83748497 83748587 83748497 ID=MS.gene65373.t1.exon3;Parent=MS.gene65373.t1
chr5.2 CDS 83748497 83748587 83748497 ID=cds.MS.gene65373.t1;Parent=MS.gene65373.t1
chr5.2 exon 83748312 83748388 83748312 ID=MS.gene65373.t1.exon4;Parent=MS.gene65373.t1
chr5.2 CDS 83748312 83748388 83748312 ID=cds.MS.gene65373.t1;Parent=MS.gene65373.t1
chr5.2 exon 83747593 83747681 83747593 ID=MS.gene65373.t1.exon5;Parent=MS.gene65373.t1
chr5.2 CDS 83747593 83747681 83747593 ID=cds.MS.gene65373.t1;Parent=MS.gene65373.t1
chr5.2 exon 83747273 83747456 83747273 ID=MS.gene65373.t1.exon6;Parent=MS.gene65373.t1
chr5.2 CDS 83747273 83747456 83747273 ID=cds.MS.gene65373.t1;Parent=MS.gene65373.t1
chr5.2 exon 83746909 83747090 83746909 ID=MS.gene65373.t1.exon7;Parent=MS.gene65373.t1
chr5.2 CDS 83746909 83747090 83746909 ID=cds.MS.gene65373.t1;Parent=MS.gene65373.t1
chr5.2 exon 83746593 83746695 83746593 ID=MS.gene65373.t1.exon8;Parent=MS.gene65373.t1
chr5.2 CDS 83746593 83746695 83746593 ID=cds.MS.gene65373.t1;Parent=MS.gene65373.t1
chr5.2 exon 83746173 83746504 83746173 ID=MS.gene65373.t1.exon9;Parent=MS.gene65373.t1
chr5.2 CDS 83746173 83746504 83746173 ID=cds.MS.gene65373.t1;Parent=MS.gene65373.t1
chr5.2 exon 83745601 83746042 83745601 ID=MS.gene65373.t1.exon10;Parent=MS.gene65373.t1
chr5.2 CDS 83745601 83746042 83745601 ID=cds.MS.gene65373.t1;Parent=MS.gene65373.t1
Gene Sequence

>MS.gene65373

ATGATGCATACACCATCATCAGTGAAGAGAAAGAAGAATCACACACACACAGACAAGTCCAGAAGACTCATCGGACTCCTTCTTGCCAGTTTCCTTCGCCGTCGTCTCATCCATCGCCTCCTCATTTCCGCCATCTCCGTTTGCCTTCTCTTTCTTTTCATCATGCTCTCACTCTTCGCTCCTTCCCCTTTTATTGTAAATGCTCTTCATCCTTCTACTTCAACTACCTTCTATTTCATTTATAATATATGCTTTTCGTCATTGCATTCTTCTGTTCTGTTCTGTGTGTAACTTTGATTGTATTTCAGGAAAATGATTTGGTTAAATCTCGTTTAGATTCTCATCAGCAGCAATCGCCATTTCACATTCCGGTAATCAAACAACCTTCTCAACTTTTTTTTTTATGCATTTTGTGTTATGTTGCTAATATCGATCTTCTTTTTGTTTATTGGAATTTGAAGGCAAATGAAGCTACTTACAGGCATGATCTATGGAATTCGAGAAACTCACAGCTTTTCTCTGCCTGTAGTAATGCCGGTGTCAACTTTGCAAGTAAACTTCATCCTAGACTCCCAGTCCTTTTTTCATCTCATTAATTGTAATTCCTATATTTAATTGCTTTTGATTGAGCGTATTTATTCATATTTCTAACTTTTGCAGAGGCTAATACCAAAACTCATCCGGATCGATACTTGTTAATCGCAACCAGTGGAGGCTTAAATCAACAGAGAACAGGGGTAATTAAGCTAAGCTTCTTTCTTAATGAACTGTGTCTGTGCTGTTGCTTTGTTTATGCAATCTTTTTAGTCAAGTAATGAATGTATAGAGCAGTCCAAGGAGGATAACTTATTAATCGGAATATGTACTTCTTTCTACATGTAACTAATTGTTTTATTTGAGTATGAATATTTCTTTCATTTGTTCAATTAGTAAACTTAATTGCATTCAAAATACTATTATATATAGAGTGGCGACCGTGGTCATTTACAAAAAGTATAGGTTGAAATCTCTACCCAATAATTATAGGTCTGCAACAAATTGGTGATTTGCTATACAGATTTTGTGTGTCCCATTCCGCTAAAAAAACACAATTAGGATTTTCTTGATTGAATTTGACGAATGAGTGGTGATACGAATTTGAATGATATATTACGTTATGTGGATGATGCAATATTTCTGGTATTGAAAAATTATAGATTGTTTGTGGGTGCAAGAATTATAGGTCTGCAAAAATTAGTGATTTGATATACAAATTTTGTATGTGCCATTCGGCTAAAATAACTCAATTAGGATTTTCTAGATCAAATTTGATGAAGTGGATGCAATATTTCTGGCATTGAAAAAATACAGATTGTTTGTGGGTGCAGATAATTGATGCTGTTGTGGCTGCCTATCTTTTGAATGCTACTCTTGTTATTCCAGAATTGGATCATACATCTTTCTGGAAAGACAACAGGTCTGTGTTTACATTTCACTTCTATCATATCCAGGATGTCTCTTAAATCACATATAATATTTTCTTTGGTAGCAGAACACATTTGTCATTGTACTCCTTAATTTGTATGTAATATTTGATTATTATTTTTTTGCAGCAATTTTTCTCAATTATTTGTTGCCGATTGGTTTATAACATCCCTTCGGAATGACATAAGAGTTGTAAAGCAGCTACCAAATATGGGAGAGAAATTTGTGACTCCATATACAGTGCGTGTTCCAAGGAAATGCACCCCCAAATGCTACGAGGGTCGCGTTTTACCAGTGCTGGTCAAGAAGCGTGTAAGTGTCTAATTAAAGGTCCCTAATCGGAACCTTTCATCAAAATTATTTTCATGTATCCCTCAATTTTATTAATAAATTTTGTGGTTCCAACATTGTAAGTTAGGTATCCAACATACTAGTTATTGTAGCAAGTTGTGTTGATGCCATATGATTGTATAACATTTAATTGCATTACACAGGTTGTTCGGCTCACGAAGTTTGACTACAGACTTTCTAATCTGCTGGATGATGATCTACAAAAGCTGAGGTGCAGGGTAAACTACCATGCTCTCAAATTTACGGATTCGATTCAGGGAATGGGAAATTTGTTGGTTGAGCGGATGAGAATGAAAAGCATGCGTTTCATCGCGCTACATTTAAGGTAAGATTCCATTTGATAATTATAACTAAATATTTGTCCCTGGTTCCTTTGATATGCCATTCTTCAGCATGTGATCAGCTAAATCCTAAAACAATAATTTACATTTTTCAAATTGATACGAAAGCTCTAGGCCAACTTCAATTTCATTTTCTTTTCCGAAGTTCAATGTGATAATTGTTGATCCAAACTGCTTGTTTTTTCTGTTTCATATAGGTTTGAACCGGATATGCTTGCATTCTCTGGTTGCTATTATGGTGGGGGAGAAAAGGAAAGAAAAGAACTTGGTGAAATTAGGAAGCGGTGGAAAAATTTACATGTAAGGTTCTATTGACATTCAAGATATCTTTATTAACTGTATGAAAATTTGCATAATCTTACTATTTGATAATTAATGTGACTGACAGGCAAGCAATCCTGAGAAAGTAAGAAGACATGGAAGGTGTCCACTTACCCCAGAGGAAGTTGGTCTTATGCTTAGAGCACTAGGTTTTGGAATTGAAACGCATTTGTATGTGGCTTCTGGAGAAATATATGGAGGCGAGGAAACCCTAGCACCCCTCAGAGCTCTCTTCCCCAACTTCCATTCAAAGGAGACCTTAGCTACCAAGGAGGAATTGGCCCCATTTGTGTCTTTCTCTTCACGCATGGCTGCGCTTGATTACATAGTTTGTGATGACAGCGACGTTTTTGTAACAAACAACAATGGGAACATGGCTAAAATTCTAGCTGGAAGAAGGTAAGCTAGTTTATTAGGGCGTGAGATTCTTCTAGCTAGTCTCATGTGCTTAATTTATTCCAAGTAGTTGGTTTCTATTTGGACAGTACTTTTCCTACACATACTGATATTCAGACTTTCTCTGTTGTAGGAGGTACTTTGGTCATAAACCTACCATCCGCCCTAACGGAAAAAAGTTGAACCCCTTATTCATGAACAAGAATAACATGACATGGCAAGAATTTGCATCTCGAGTTCGAACTTTCCAGGTAGGCTTCATGGGAGAGCCTAATGAGTTGAGGTCAGGCAGTGGTGAGTTTCACGAGAATCCAACGTCTTGCATATGTCAAAACTCAGGGTCGGAGGTAAAAACTGAGGGATTTTCCAATCCTCAAAATCAAACGCATGAAGGCAATAAGGTAGAAAACAGGACAGAAAACCAGCCTGCTGAGGAAGTACGAGAGTGGTCTGAATTAGATTATGATTTGGACAATTATAGAAAGCAGGTTCAGCTCAAAGGAACTAATATTGAGTCGCTCCCCCTTCTAATGGGTACTGACCAAGCTGAAGTGCAAGTATTCTTCTCAGATTAG
Protein sequence

>MS.gene65373.t1

MMHTPSSVKRKKNHTHTDKSRRLIGLLLASFLRRRLIHRLLISAISVCLLFLFIMLSLFAPSPFIENDLVKSRLDSHQQQSPFHIPANEATYRHDLWNSRNSQLFSACSNAGVNFAKANTKTHPDRYLLIATSGGLNQQRTGIIDAVVAAYLLNATLVIPELDHTSFWKDNSNFSQLFVADWFITSLRNDIRVVKQLPNMGEKFVTPYTVRVPRKCTPKCYEGRVLPVLVKKRVVRLTKFDYRLSNLLDDDLQKLRCRVNYHALKFTDSIQGMGNLLVERMRMKSMRFIALHLRFEPDMLAFSGCYYGGGEKERKELGEIRKRWKNLHASNPEKVRRHGRCPLTPEEVGLMLRALGFGIETHLYVASGEIYGGEETLAPLRALFPNFHSKETLATKEELAPFVSFSSRMAALDYIVCDDSDVFVTNNNGNMAKILAGRRRYFGHKPTIRPNGKKLNPLFMNKNNMTWQEFASRVRTFQVGFMGEPNELRSGSGEFHENPTSCICQNSGSEVKTEGFSNPQNQTHEGNKVENRTENQPAEEVREWSELDYDLDNYRKQVQLKGTNIESLPLLMGTDQAEVQVFFSD