Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65548.t1 | XP_013451637.1 | 95.4 | 696 | 29 | 2 | 1 | 695 | 1 | 694 | 0.00E+00 | 1261.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65548.t1 | Q9ZPV5 | 37.6 | 489 | 289 | 7 | 188 | 666 | 129 | 611 | 2.0e-87 | 325.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65548.t1 | A0A072UIM4 | 95.4 | 696 | 29 | 2 | 1 | 695 | 1 | 694 | 0.0e+00 | 1261.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05689 | MS.gene65548 | 0.856777 | 2.56E-62 | -1.69E-46 |
| MS.gene059935 | MS.gene65548 | 0.826633 | 2.44E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65548.t1 | MTR_6g033410 | 94.730 | 721 | 35 | 2 | 1 | 720 | 1 | 719 | 0.0 | 1355 |
| MS.gene65548.t1 | MTR_1g110560 | 33.110 | 598 | 365 | 9 | 96 | 666 | 6 | 595 | 1.20e-102 | 331 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65548.t1 | AT3G55510 | 47.834 | 554 | 273 | 5 | 123 | 663 | 35 | 585 | 2.69e-176 | 517 |
| MS.gene65548.t1 | AT3G55510 | 51.579 | 95 | 44 | 2 | 1 | 94 | 1 | 94 | 6.68e-17 | 85.1 |
| MS.gene65548.t1 | AT3G55510 | 49.703 | 505 | 246 | 3 | 164 | 663 | 9 | 510 | 9.99e-174 | 508 |
| MS.gene65548.t1 | AT2G18220 | 36.216 | 555 | 321 | 8 | 139 | 666 | 63 | 611 | 7.76e-115 | 363 |
Find 124 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAGCTTCAATGATCTTTC+TGG | 0.145570 | 6.4:+5926371 | MS.gene65548:CDS |
| GGGGTTGCTCTCCTCTTTCC+TGG | 0.187540 | 6.4:+5927452 | MS.gene65548:CDS |
| ATGATTGCTCAAATTATAAA+TGG | 0.217503 | 6.4:+5927431 | MS.gene65548:CDS |
| ACATCAACTGCATTGATCTT+TGG | 0.231659 | 6.4:+5927354 | MS.gene65548:CDS |
| TGAACCTTTGTCTACCATTA+AGG | 0.244522 | 6.4:+5927631 | MS.gene65548:CDS |
| AATCACCGAATGGGAAAATT+AGG | 0.245090 | 6.4:+5922733 | None:intergenic |
| TTCAAAGAAGGAAGCTGTTT+TGG | 0.258857 | 6.4:+5926224 | MS.gene65548:CDS |
| TGCAGATTTGCGTTGATCTC+TGG | 0.266493 | 6.4:+5926946 | MS.gene65548:intron |
| CCTCTTTGTAATTGCTTTCC+TGG | 0.268015 | 6.4:-5929366 | None:intergenic |
| GATGGCAACCCTACTGATTC+TGG | 0.289709 | 6.4:+5929883 | MS.gene65548:CDS |
| CATCAACTGCATTGATCTTT+GGG | 0.301734 | 6.4:+5927355 | MS.gene65548:CDS |
| TGCGTTGATCTCTGGGTAAC+AGG | 0.312426 | 6.4:+5926954 | MS.gene65548:CDS |
| TGTCGATTCTTTGTGTAAAC+TGG | 0.328689 | 6.4:+5925993 | MS.gene65548:CDS |
| TCTTGAAATCTACAGTTTCC+AGG | 0.333254 | 6.4:+5924317 | MS.gene65548:intron |
| ATCATCACTTCCGGTAATAT+CGG | 0.341033 | 6.4:-5923980 | None:intergenic |
| GATGGCAACCCTACTGATTC+CGG | 0.347696 | 6.4:+5929823 | MS.gene65548:CDS |
| ATCTAAAGAATAAAGCTAAA+TGG | 0.358025 | 6.4:+5926247 | MS.gene65548:CDS |
| CAATGGCTTAATCGTCTTGC+CGG | 0.364825 | 6.4:+5927509 | MS.gene65548:CDS |
| TGGATGAGACACACTCTTCT+TGG | 0.367727 | 6.4:-5927843 | None:intergenic |
| TCGTTTGAAACTCTCATTAG+AGG | 0.368893 | 6.4:-5927971 | None:intergenic |
| AGTGGAGCTGTTGCCAGTTC+GGG | 0.371151 | 6.4:-5927919 | None:intergenic |
| CATCCTAATGGCGTTGTTGA+TGG | 0.372443 | 6.4:+5929805 | MS.gene65548:CDS |
| TTTCTCTTCTTTCCTTTGCC+TGG | 0.372496 | 6.4:-5929778 | None:intergenic |
| TCTTTGGGTTGCATTTATAT+CGG | 0.374098 | 6.4:+5927370 | MS.gene65548:CDS |
| TCCAGCACGAATGATGTAAC+AGG | 0.376980 | 6.4:-5927551 | None:intergenic |
| AAGTGGAGCTGTTGCCAGTT+CGG | 0.384071 | 6.4:-5927920 | None:intergenic |
| GTCTGTCGAAACGTTTCTTC+AGG | 0.390890 | 6.4:+5929153 | MS.gene65548:CDS |
| AGTTCCTTAGAAAATTTGTA+TGG | 0.392486 | 6.4:-5927103 | None:intergenic |
| TATCAAGCGTTTCATTGATC+AGG | 0.396178 | 6.4:+5927996 | MS.gene65548:CDS |
| TGATTGCTCAAATTATAAAT+GGG | 0.399691 | 6.4:+5927432 | MS.gene65548:CDS |
| TCCAGGTTTCCCACCATCCT+TGG | 0.400233 | 6.4:-5927594 | None:intergenic |
| CATCCCAATGGCGTAGTTGA+TGG | 0.409710 | 6.4:+5929865 | MS.gene65548:CDS |
| TCTAGCATTGCCGATATTAC+CGG | 0.413671 | 6.4:+5923970 | MS.gene65548:CDS |
| TCATGCGCAAGGCTGCTTCC+AGG | 0.425550 | 6.4:+5929348 | MS.gene65548:CDS |
| TTGCCATCAACAACGCCATT+AGG | 0.426689 | 6.4:-5929808 | None:intergenic |
| CTTAAGTATGACTTAATAAG+AGG | 0.433292 | 6.4:-5926282 | None:intergenic |
| AATGATGAAACTGAAGTTCT+TGG | 0.442169 | 6.4:+5923063 | MS.gene65548:CDS |
| GGGTTGCTCTCCTCTTTCCT+GGG | 0.442425 | 6.4:+5927453 | MS.gene65548:CDS |
| TCGACAGACTGCTGATCCTT+CGG | 0.446504 | 6.4:-5929139 | None:intergenic |
| TGCTGATCCTTCGGTGAGAA+AGG | 0.448345 | 6.4:-5929130 | None:intergenic |
| GCAGATTTGCGTTGATCTCT+GGG | 0.448387 | 6.4:+5926947 | MS.gene65548:intron |
| ACTGTGGCATGCCGCTCTAT+AGG | 0.449554 | 6.4:-5926061 | None:intergenic |
| ATGAAGCTGATATTATGTTT+AGG | 0.452732 | 6.4:+5926175 | MS.gene65548:CDS |
| GTTGAACAAATTGAAAGAAA+AGG | 0.452990 | 6.4:+5924195 | MS.gene65548:CDS |
| CCCTGTTACATCATTCGTGC+TGG | 0.453632 | 6.4:+5927550 | MS.gene65548:CDS |
| AGATTGAGGGCGACCGATGA+AGG | 0.455714 | 6.4:-5927078 | None:intergenic |
| ACCAAGGATGGTGGGAAACC+TGG | 0.462289 | 6.4:+5927593 | MS.gene65548:CDS |
| GATGAAAATCCCACTAGAAC+CGG | 0.462503 | 6.4:-5927528 | None:intergenic |
| AGGCATTTATGAGACAAGTA+AGG | 0.462603 | 6.4:-5926041 | None:intergenic |
| ACTCTCAGTGGCAAATATCT+AGG | 0.464919 | 6.4:-5927476 | None:intergenic |
| CTCTTATTAAGTCATACTTA+AGG | 0.467323 | 6.4:+5926283 | MS.gene65548:CDS |
| TAATCTATGCTGCAGTCTCC+AGG | 0.467895 | 6.4:+5929760 | MS.gene65548:intron |
| ACAAGCTTGTGCAGTAAAGA+TGG | 0.468827 | 6.4:-5926402 | None:intergenic |
| AAATTTGTATGGGCAGATTG+AGG | 0.474241 | 6.4:-5927092 | None:intergenic |
| AATCGTCTTGCCGGTTCTAG+TGG | 0.475650 | 6.4:+5927518 | MS.gene65548:CDS |
| ATATCTCAGAGTCGGTGATC+TGG | 0.486421 | 6.4:-5926326 | None:intergenic |
| TCCTTCTTTGAACTCGAGCT+TGG | 0.488161 | 6.4:-5926213 | None:intergenic |
| TGGTTAATAAAATTTGTAGT+TGG | 0.488967 | 6.4:+5927327 | MS.gene65548:CDS |
| TTGCCATCAACTACGCCATT+GGG | 0.491575 | 6.4:-5929868 | None:intergenic |
| AAGAGAAAGCAGCATCCTAA+TGG | 0.491744 | 6.4:+5929793 | MS.gene65548:CDS |
| AATTTGTATGGGCAGATTGA+GGG | 0.498650 | 6.4:-5927091 | None:intergenic |
| TTCACTCGTCTGAATGCCGT+TGG | 0.498889 | 6.4:-5926121 | None:intergenic |
| GTTGCCATCAACTACGCCAT+TGG | 0.504206 | 6.4:-5929869 | None:intergenic |
| CGGCAAGACGATTAAGCCAT+TGG | 0.511519 | 6.4:-5927508 | None:intergenic |
| TTCAAAAGAAAAGCTGCTAA+AGG | 0.512057 | 6.4:+5922829 | MS.gene65548:CDS |
| CTTGAAATCTACAGTTTCCA+GGG | 0.514226 | 6.4:+5924318 | MS.gene65548:intron |
| AATATCTAGGCCCAGGAAAG+AGG | 0.514834 | 6.4:-5927463 | None:intergenic |
| ACAAAGCAATGACATGCATA+AGG | 0.523663 | 6.4:+5927168 | MS.gene65548:CDS |
| TATATCTCGTATCATAAGAA+AGG | 0.523951 | 6.4:-5927000 | None:intergenic |
| ACCAAGCTCGAGTTCAAAGA+AGG | 0.525083 | 6.4:+5926212 | MS.gene65548:CDS |
| ATGCTCTGAAAGAAGTTCAA+TGG | 0.526512 | 6.4:-5927866 | None:intergenic |
| TAATTCTTTCCAGAATCAGT+AGG | 0.531955 | 6.4:-5929892 | None:intergenic |
| TACAATATTACCAAGGATGG+TGG | 0.536875 | 6.4:+5927584 | MS.gene65548:CDS |
| CATCTATGTGTAGCTCTAAG+TGG | 0.539418 | 6.4:+5927024 | MS.gene65548:CDS |
| ATCGTCTTGCCGGTTCTAGT+GGG | 0.544915 | 6.4:+5927519 | MS.gene65548:CDS |
| AGTGGCAAATATCTAGGCCC+AGG | 0.553627 | 6.4:-5927470 | None:intergenic |
| AATTCTTTCCAGAATCAGTA+GGG | 0.559311 | 6.4:-5929891 | None:intergenic |
| AGCAATTACAAAGAGGAAGA+AGG | 0.565765 | 6.4:+5929373 | MS.gene65548:CDS |
| TAGTGATGGCTTTCTTGAAG+AGG | 0.565826 | 6.4:+5923101 | MS.gene65548:CDS |
| ATGCTGCAGTCTCCAGGCAA+AGG | 0.566597 | 6.4:+5929766 | MS.gene65548:intron |
| TCTAGGTGACATATTGCTGA+AGG | 0.568267 | 6.4:+5927193 | MS.gene65548:CDS |
| AAAACATACAAGGCCTTCAT+CGG | 0.576724 | 6.4:+5927065 | MS.gene65548:CDS |
| ATGATGAAAATGCACATACT+CGG | 0.577479 | 6.4:+5925944 | MS.gene65548:CDS |
| ATGACATGCATAAGGCGTCT+AGG | 0.578999 | 6.4:+5927176 | MS.gene65548:CDS |
| ACAATATTACCAAGGATGGT+GGG | 0.580245 | 6.4:+5927585 | MS.gene65548:CDS |
| GGCATTTATGAGACAAGTAA+GGG | 0.582202 | 6.4:-5926040 | None:intergenic |
| GCCAGAAATTTAGAAAACCC+TGG | 0.583477 | 6.4:-5924335 | None:intergenic |
| CTACAAAAGCATCATGCGCA+AGG | 0.584364 | 6.4:+5929337 | MS.gene65548:CDS |
| GAATACAATATTACCAAGGA+TGG | 0.584476 | 6.4:+5927581 | MS.gene65548:CDS |
| GTTCCTTAGAAAATTTGTAT+GGG | 0.585756 | 6.4:-5927102 | None:intergenic |
| GATTGCTCAAATTATAAATG+GGG | 0.589673 | 6.4:+5927433 | MS.gene65548:CDS |
| TAAGACACTGCCACTGACAT+TGG | 0.595386 | 6.4:-5926097 | None:intergenic |
| TTTATGAGACAAGTAAGGGC+AGG | 0.596259 | 6.4:-5926036 | None:intergenic |
| CTAGGTGACATATTGCTGAA+GGG | 0.596365 | 6.4:+5927194 | MS.gene65548:CDS |
| TTTACTGCACAAGCTTGTCA+AGG | 0.596699 | 6.4:+5926407 | MS.gene65548:CDS |
| GAAAATGCACATACTCGGGT+GGG | 0.601534 | 6.4:+5925949 | MS.gene65548:CDS |
| AGTGGCAGTGTCTTATCCAA+CGG | 0.603640 | 6.4:+5926105 | MS.gene65548:CDS |
| TAGGTGACATATTGCTGAAG+GGG | 0.605004 | 6.4:+5927195 | MS.gene65548:CDS |
| TGACGTGCCTTTCTCACCGA+AGG | 0.610094 | 6.4:+5929123 | MS.gene65548:CDS |
| GATCTGGTTCAAGAGAAATA+CGG | 0.610235 | 6.4:-5926310 | None:intergenic |
| TGAAAATGCACATACTCGGG+TGG | 0.615078 | 6.4:+5925948 | MS.gene65548:CDS |
| GATGAAACTGAAGTTCTTGG+TGG | 0.616113 | 6.4:+5923066 | MS.gene65548:CDS |
| GTGACATATTGCTGAAGGGG+TGG | 0.621378 | 6.4:+5927198 | MS.gene65548:CDS |
| ATCTTCTTAAGGTAGACAAG+TGG | 0.622045 | 6.4:-5927937 | None:intergenic |
| GGTGATGATTCTGATAGTGA+TGG | 0.623236 | 6.4:+5923087 | MS.gene65548:CDS |
| CTCTGAAAGAAGTTCAATGG+TGG | 0.623308 | 6.4:-5927863 | None:intergenic |
| TGAGAGTCAGATGTATCCAA+TGG | 0.624233 | 6.4:+5927492 | MS.gene65548:CDS |
| TCTCGAATACAATATTACCA+AGG | 0.625318 | 6.4:+5927577 | MS.gene65548:CDS |
| GAAGGCTAATATCTCAGAGT+CGG | 0.631890 | 6.4:-5926334 | None:intergenic |
| TGATGAAAATGCACATACTC+GGG | 0.632504 | 6.4:+5925945 | MS.gene65548:CDS |
| TGAGCAATCATATACAACAA+TGG | 0.635764 | 6.4:-5927419 | None:intergenic |
| CTTACCTTAATGGTAGACAA+AGG | 0.637847 | 6.4:-5927635 | None:intergenic |
| CCAGGAAAGCAATTACAAAG+AGG | 0.641381 | 6.4:+5929366 | MS.gene65548:CDS |
| AGTTATGATGATGAAGACAC+TGG | 0.643215 | 6.4:+5924359 | MS.gene65548:CDS |
| CCAGCACGAATGATGTAACA+GGG | 0.652463 | 6.4:-5927550 | None:intergenic |
| CCAGTTCGGGAAAAGACACA+TGG | 0.661527 | 6.4:-5927906 | None:intergenic |
| TCTGAGTCGACAGATTGAGA+AGG | 0.665264 | 6.4:-5926352 | None:intergenic |
| TCTGAAACAACCAATGTCAG+TGG | 0.666002 | 6.4:+5926087 | MS.gene65548:CDS |
| AAGAAAACACAACATCCCAA+TGG | 0.672006 | 6.4:+5929853 | MS.gene65548:CDS |
| TCTCATAAATGCCTATAGAG+CGG | 0.688586 | 6.4:+5926050 | MS.gene65548:CDS |
| TGACAGGCGGAGAAACGTAG+TGG | 0.691324 | 6.4:+5929293 | MS.gene65548:intron |
| TGAAGAGCATAGATCCACAA+AGG | 0.694028 | 6.4:-5927126 | None:intergenic |
| TGGATACATCTGACTCTCAG+TGG | 0.694892 | 6.4:-5927488 | None:intergenic |
| TGGTTGTTTCAGAATCACTG+TGG | 0.728435 | 6.4:-5926077 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 5922742 | 5929915 | 5922742 | ID=MS.gene65548 |
| chr6.4 | mRNA | 5922742 | 5929915 | 5922742 | ID=MS.gene65548.t1;Parent=MS.gene65548 |
| chr6.4 | exon | 5922742 | 5922850 | 5922742 | ID=MS.gene65548.t1.exon1;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5922742 | 5922850 | 5922742 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5922950 | 5923122 | 5922950 | ID=MS.gene65548.t1.exon2;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5922950 | 5923122 | 5922950 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5923967 | 5924024 | 5923967 | ID=MS.gene65548.t1.exon3;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5923967 | 5924024 | 5923967 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5924122 | 5924216 | 5924122 | ID=MS.gene65548.t1.exon4;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5924122 | 5924216 | 5924122 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5924332 | 5924403 | 5924332 | ID=MS.gene65548.t1.exon5;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5924332 | 5924403 | 5924332 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5925922 | 5926428 | 5925922 | ID=MS.gene65548.t1.exon6;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5925922 | 5926428 | 5925922 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5926951 | 5927235 | 5926951 | ID=MS.gene65548.t1.exon7;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5926951 | 5927235 | 5926951 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5927323 | 5927652 | 5927323 | ID=MS.gene65548.t1.exon8;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5927323 | 5927652 | 5927323 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5927811 | 5928017 | 5927811 | ID=MS.gene65548.t1.exon9;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5927811 | 5928017 | 5927811 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5929085 | 5929174 | 5929085 | ID=MS.gene65548.t1.exon10;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5929085 | 5929174 | 5929085 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5929299 | 5929394 | 5929299 | ID=MS.gene65548.t1.exon11;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5929299 | 5929394 | 5929299 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| chr6.4 | exon | 5929775 | 5929915 | 5929775 | ID=MS.gene65548.t1.exon12;Parent=MS.gene65548.t1 |
| chr6.4 | CDS | 5929775 | 5929915 | 5929775 | ID=cds.MS.gene65548.t1;Parent=MS.gene65548.t1 |
| Gene Sequence |
| Protein sequence |
