Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65556.t1 | XP_013451663.1 | 98.7 | 307 | 3 | 1 | 17 | 323 | 15 | 320 | 9.80E-171 | 609.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65556.t1 | Q8GW31 | 76.6 | 273 | 63 | 1 | 18 | 290 | 18 | 289 | 2.5e-122 | 439.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65556.t1 | A0A072U8J7 | 98.7 | 307 | 3 | 1 | 17 | 323 | 15 | 320 | 7.1e-171 | 609.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene65556 | -0.804445 | 2.15E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene65556 | -0.826767 | 2.26E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene65556 | -0.813242 | 2.83E-51 | -1.69E-46 |
| MS.gene056830 | MS.gene65556 | 0.816644 | 4.97E-52 | -1.69E-46 |
| MS.gene057705 | MS.gene65556 | -0.80271 | 4.93E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65556.t1 | MTR_6g033535 | 98.142 | 323 | 3 | 3 | 1 | 323 | 1 | 320 | 0.0 | 639 |
| MS.gene65556.t1 | MTR_6g033535 | 98.644 | 295 | 3 | 1 | 29 | 323 | 15 | 308 | 0.0 | 593 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65556.t1 | AT5G13570 | 61.394 | 373 | 93 | 3 | 1 | 323 | 1 | 372 | 2.71e-161 | 455 |
| MS.gene65556.t1 | AT5G13570 | 59.326 | 386 | 93 | 4 | 1 | 323 | 1 | 385 | 9.12e-158 | 446 |
Find 67 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTATATGGTGTCTCCCTTTC+TGG | 0.137033 | 6.4:-5560308 | MS.gene65556:CDS |
| AGACCTGATTTGCCACTTAA+AGG | 0.227358 | 6.4:-5560154 | MS.gene65556:intron |
| AGAATTATTGCCCCAGTTAC+AGG | 0.269034 | 6.4:+5564260 | None:intergenic |
| AATGCAGATCTTTGAAATCT+TGG | 0.274871 | 6.4:-5560207 | MS.gene65556:intron |
| TTGACAGTGTTTGCTAGTTA+AGG | 0.284066 | 6.4:-5563306 | MS.gene65556:intron |
| AAGGTTCGAGTTCCTGTAAC+TGG | 0.310426 | 6.4:-5564272 | MS.gene65556:CDS |
| CACTGGTCTTAAGCTGTATA+TGG | 0.326583 | 6.4:-5560323 | MS.gene65556:CDS |
| AAGTAGTTCCACAATAATTA+TGG | 0.344465 | 6.4:-5558780 | MS.gene65556:CDS |
| ATTTGCCCCACAAACCAAAA+AGG | 0.347992 | 6.4:-5562333 | MS.gene65556:CDS |
| CAGCCTATCGATGTGGGGCC+TGG | 0.359264 | 6.4:-5558725 | MS.gene65556:CDS |
| ATGCAGGTCTTGGAAGAAAC+TGG | 0.365507 | 6.4:-5562461 | MS.gene65556:intron |
| GCTTCCATTGCTTGTAGAAT+AGG | 0.371445 | 6.4:+5558668 | None:intergenic |
| ATACCTTTAAGTGGCAAATC+AGG | 0.382526 | 6.4:+5560151 | None:intergenic |
| TGGGGCCTGGCCGGAGCTTA+AGG | 0.393086 | 6.4:-5558712 | MS.gene65556:CDS |
| AATGATTGAAGATCTTCCTT+TGG | 0.395909 | 6.4:+5565285 | None:intergenic |
| AAAGCAAAGCACAGTTCTAC+TGG | 0.422996 | 6.4:-5558803 | MS.gene65556:CDS |
| CGAAAGGATCTTATTTCTTG+TGG | 0.433621 | 6.4:-5565262 | MS.gene65556:CDS |
| TGACAGTGTTTGCTAGTTAA+GGG | 0.433791 | 6.4:-5563305 | MS.gene65556:intron |
| ACATCGATAGGCTGAGAATC+TGG | 0.440402 | 6.4:+5558734 | None:intergenic |
| TTAAGTGGCAAATCAGGTCT+TGG | 0.443206 | 6.4:+5560157 | None:intergenic |
| AGGGTTGGAAAGGTTCAAGT+TGG | 0.451483 | 6.4:-5563286 | MS.gene65556:CDS |
| CTATCGATGTGGGGCCTGGC+CGG | 0.452979 | 6.4:-5558721 | MS.gene65556:CDS |
| TCTTGATGAAACCTTTGAAA+GGG | 0.457863 | 6.4:-5564240 | MS.gene65556:intron |
| GTACGACTCTACATTATTGC+TGG | 0.465038 | 6.4:-5562374 | MS.gene65556:CDS |
| TTCAACACACCAACCTCCTA+TGG | 0.469327 | 6.4:-5560182 | MS.gene65556:CDS |
| GATATCACATCATCACTAGA+AGG | 0.473059 | 6.4:+5560355 | None:intergenic |
| GTTGCGGGACTTTCAAGAAA+AGG | 0.490549 | 6.4:+5558643 | None:intergenic |
| TCATCTATATGGGCCACATA+AGG | 0.495540 | 6.4:+5564320 | None:intergenic |
| TACAGGAACTCGAACCTTGT+AGG | 0.496480 | 6.4:+5564277 | None:intergenic |
| GTGATATCTCGTGGGATCAC+TGG | 0.499501 | 6.4:-5560340 | MS.gene65556:CDS |
| CGGCCAGGCCCCACATCGAT+AGG | 0.500991 | 6.4:+5558722 | None:intergenic |
| ATCTGAAATTCCTTAAGCTC+CGG | 0.502196 | 6.4:+5558702 | None:intergenic |
| TTGGCACATTTAGCACAAAC+CGG | 0.502442 | 6.4:+5565304 | None:intergenic |
| AGTGTTTGCTAGTTAAGGGT+TGG | 0.504029 | 6.4:-5563301 | MS.gene65556:CDS |
| TTCTCAGCTTACCCTTTCAA+AGG | 0.507737 | 6.4:+5564229 | None:intergenic |
| TAAGGACTTCACTTCCTACA+AGG | 0.509912 | 6.4:-5564291 | MS.gene65556:CDS |
| AACTCCTCGATGATCTCTGC+AGG | 0.514488 | 6.4:-5567274 | MS.gene65556:intron |
| ACATAGGAAATACCTTTAAG+TGG | 0.517044 | 6.4:+5560142 | None:intergenic |
| AGTTGTACTCACGCCAGAAA+GGG | 0.521072 | 6.4:+5560295 | None:intergenic |
| TTGCTAGTTAAGGGTTGGAA+AGG | 0.537004 | 6.4:-5563296 | MS.gene65556:CDS |
| TATCTTTACAGGAAATTGCA+TGG | 0.538158 | 6.4:-5560399 | MS.gene65556:intron |
| AGACTTAAAGACTTCAGAGA+TGG | 0.542098 | 6.4:+5565195 | None:intergenic |
| TTCTTGATGAAACCTTTGAA+AGG | 0.543629 | 6.4:-5564241 | MS.gene65556:CDS |
| AAATCAGGTCTTGGAGCCAT+AGG | 0.545316 | 6.4:+5560166 | None:intergenic |
| AGGTTCGAGTTCCTGTAACT+GGG | 0.545714 | 6.4:-5564271 | MS.gene65556:CDS |
| TCTGAAGTCTTTAAGTCTGA+AGG | 0.553893 | 6.4:-5565190 | MS.gene65556:CDS |
| TGTTTCAAAATTTCTTAACA+AGG | 0.554360 | 6.4:-5562432 | MS.gene65556:CDS |
| AGATCATCGAGGAGTTCTTG+AGG | 0.556196 | 6.4:+5567281 | None:intergenic |
| GTCTTGGAGCCATAGGAGGT+TGG | 0.558406 | 6.4:+5560173 | None:intergenic |
| TGCACCTATTCTACAAGCAA+TGG | 0.558651 | 6.4:-5558672 | MS.gene65556:CDS |
| AAGTTGTACTCACGCCAGAA+AGG | 0.559904 | 6.4:+5560294 | None:intergenic |
| GATTCTCAGCCTATCGATGT+GGG | 0.563930 | 6.4:-5558731 | MS.gene65556:CDS |
| TTGGCTGTCCATAATTATTG+TGG | 0.564380 | 6.4:+5558772 | None:intergenic |
| AAGATCTTCAATCATTCGAA+AGG | 0.567710 | 6.4:-5565278 | MS.gene65556:CDS |
| AAATTCCTTAAGCTCCGGCC+AGG | 0.571195 | 6.4:+5558707 | None:intergenic |
| GAGTTCTTGAGGAGGAAGAA+CGG | 0.571382 | 6.4:+5567292 | None:intergenic |
| TTTATCAAGGAATTTGTGTG+TGG | 0.584014 | 6.4:-5558826 | MS.gene65556:intron |
| TTCTTGTGGAGTATGCACAT+TGG | 0.597459 | 6.4:-5565248 | MS.gene65556:CDS |
| TCAGGTCTTGGAGCCATAGG+AGG | 0.601393 | 6.4:+5560169 | None:intergenic |
| GTTTGTGCTAAATGTGCCAA+AGG | 0.616660 | 6.4:-5565301 | MS.gene65556:CDS |
| AGTGATGATGTGATATCTCG+TGG | 0.625983 | 6.4:-5560349 | MS.gene65556:CDS |
| AGATTCTCAGCCTATCGATG+TGG | 0.642684 | 6.4:-5558732 | MS.gene65556:CDS |
| GGTTCGAGTTCCTGTAACTG+GGG | 0.643999 | 6.4:-5564270 | MS.gene65556:CDS |
| TCATCGAGGAGTTCTTGAGG+AGG | 0.644982 | 6.4:+5567284 | None:intergenic |
| ATTCTCAGCCTATCGATGTG+GGG | 0.672563 | 6.4:-5558730 | MS.gene65556:CDS |
| GTGATGATGTGATATCTCGT+GGG | 0.691549 | 6.4:-5560348 | MS.gene65556:CDS |
| CGAACCTGCAGAGATCATCG+AGG | 0.758435 | 6.4:+5567270 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 5558655 | 5567360 | 5558655 | ID=MS.gene65556 |
| chr6.4 | mRNA | 5558655 | 5567360 | 5558655 | ID=MS.gene65556.t1;Parent=MS.gene65556 |
| chr6.4 | exon | 5567275 | 5567360 | 5567275 | ID=MS.gene65556.t1.exon1;Parent=MS.gene65556.t1 |
| chr6.4 | CDS | 5567275 | 5567360 | 5567275 | ID=cds.MS.gene65556.t1;Parent=MS.gene65556.t1 |
| chr6.4 | exon | 5565175 | 5565326 | 5565175 | ID=MS.gene65556.t1.exon2;Parent=MS.gene65556.t1 |
| chr6.4 | CDS | 5565175 | 5565326 | 5565175 | ID=cds.MS.gene65556.t1;Parent=MS.gene65556.t1 |
| chr6.4 | exon | 5564241 | 5564368 | 5564241 | ID=MS.gene65556.t1.exon3;Parent=MS.gene65556.t1 |
| chr6.4 | CDS | 5564241 | 5564368 | 5564241 | ID=cds.MS.gene65556.t1;Parent=MS.gene65556.t1 |
| chr6.4 | exon | 5563226 | 5563321 | 5563226 | ID=MS.gene65556.t1.exon4;Parent=MS.gene65556.t1 |
| chr6.4 | CDS | 5563226 | 5563321 | 5563226 | ID=cds.MS.gene65556.t1;Parent=MS.gene65556.t1 |
| chr6.4 | exon | 5562325 | 5562477 | 5562325 | ID=MS.gene65556.t1.exon5;Parent=MS.gene65556.t1 |
| chr6.4 | CDS | 5562325 | 5562477 | 5562325 | ID=cds.MS.gene65556.t1;Parent=MS.gene65556.t1 |
| chr6.4 | exon | 5560307 | 5560410 | 5560307 | ID=MS.gene65556.t1.exon6;Parent=MS.gene65556.t1 |
| chr6.4 | CDS | 5560307 | 5560410 | 5560307 | ID=cds.MS.gene65556.t1;Parent=MS.gene65556.t1 |
| chr6.4 | exon | 5560155 | 5560222 | 5560155 | ID=MS.gene65556.t1.exon7;Parent=MS.gene65556.t1 |
| chr6.4 | CDS | 5560155 | 5560222 | 5560155 | ID=cds.MS.gene65556.t1;Parent=MS.gene65556.t1 |
| chr6.4 | exon | 5558655 | 5558839 | 5558655 | ID=MS.gene65556.t1.exon8;Parent=MS.gene65556.t1 |
| chr6.4 | CDS | 5558655 | 5558839 | 5558655 | ID=cds.MS.gene65556.t1;Parent=MS.gene65556.t1 |
| Gene Sequence |
| Protein sequence |
