Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65612.t1 | AES69043.1 | 85.1 | 47 | 7 | 0 | 13 | 59 | 55 | 101 | 1.70E-12 | 81.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65612.t1 | G7IWA0 | 85.1 | 47 | 7 | 0 | 13 | 59 | 55 | 101 | 1.2e-12 | 81.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056167 | MS.gene65612 | 0.812311 | 4.52E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65612.t1 | MTR_3g021000 | 85.106 | 47 | 7 | 0 | 13 | 59 | 55 | 101 | 1.71e-22 | 82.4 |
| MS.gene65612.t1 | MTR_3g434840 | 65.306 | 49 | 16 | 1 | 12 | 59 | 321 | 369 | 1.97e-13 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 4 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCAAAACTATCAGCATT+AGG | 0.396315 | 8.2:+37767342 | None:intergenic |
| TTAATAGTAGCAAGTAATTG+AGG | 0.567063 | 8.2:+37767441 | None:intergenic |
| TATTGTGATAGAGCTACTCA+CGG | 0.643043 | 8.2:-37767379 | MS.gene65612:CDS |
| AGCACATATGAATGAGCATG+TGG | 0.733236 | 8.2:-37767412 | MS.gene65612:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTAATAGTAGCAAGTAATTG+AGG | + | chr8.2:37767455-37767474 | None:intergenic | 25.0% |
| ACTTCAAAACTATCAGCATT+AGG | + | chr8.2:37767554-37767573 | None:intergenic | 30.0% | |
| CCAATTAGAAGCAAATGAAA+AGG | + | chr8.2:37767411-37767430 | None:intergenic | 30.0% | |
| ! | CCTTTTCATTTGCTTCTAAT+TGG | - | chr8.2:37767408-37767427 | MS.gene65612:CDS | 30.0% |
| !!! | GATAGTTTTGAAGTAGTTGT+TGG | - | chr8.2:37767560-37767579 | MS.gene65612:CDS | 30.0% |
| CAGCAAAGGAAATTACTTCA+GGG | + | chr8.2:37767352-37767371 | None:intergenic | 35.0% | |
| TATTGTGATAGAGCTACTCA+CGG | - | chr8.2:37767514-37767533 | MS.gene65612:intron | 35.0% | |
| TCAGCAAAGGAAATTACTTC+AGG | + | chr8.2:37767353-37767372 | None:intergenic | 35.0% | |
| ! | AAGGCAAGTAAGAGTACAAT+TGG | + | chr8.2:37767392-37767411 | None:intergenic | 35.0% |
| ! | AGGCAAGTAAGAGTACAATT+GGG | + | chr8.2:37767391-37767410 | None:intergenic | 35.0% |
| AGCACATATGAATGAGCATG+TGG | - | chr8.2:37767481-37767500 | MS.gene65612:intron | 40.0% | |
| GAGAACAACAATCTCAGCAA+AGG | + | chr8.2:37767366-37767385 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 37767320 | 37767595 | 37767320 | ID=MS.gene65612 |
| chr8.2 | mRNA | 37767320 | 37767595 | 37767320 | ID=MS.gene65612.t1;Parent=MS.gene65612 |
| chr8.2 | exon | 37767560 | 37767595 | 37767560 | ID=MS.gene65612.t1.exon1;Parent=MS.gene65612.t1 |
| chr8.2 | CDS | 37767560 | 37767595 | 37767560 | ID=cds.MS.gene65612.t1;Parent=MS.gene65612.t1 |
| chr8.2 | exon | 37767320 | 37767463 | 37767320 | ID=MS.gene65612.t1.exon2;Parent=MS.gene65612.t1 |
| chr8.2 | CDS | 37767320 | 37767463 | 37767320 | ID=cds.MS.gene65612.t1;Parent=MS.gene65612.t1 |
| Gene Sequence |
| Protein sequence |
