Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65757.t1 | RHN51483.1 | 69.2 | 182 | 24 | 3 | 16 | 165 | 437 | 618 | 1.20E-63 | 252.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65757.t1 | A0A396HFX6 | 69.2 | 182 | 24 | 3 | 16 | 165 | 437 | 618 | 8.6e-64 | 252.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051594 | MS.gene65757 | 0.809006 | 2.34E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene65757 | 0.801196 | 1.01E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65757.t1 | MTR_5g077900 | 70.286 | 175 | 22 | 2 | 19 | 163 | 50 | 224 | 8.04e-82 | 241 |
| MS.gene65757.t1 | MTR_0002s1270 | 70.857 | 175 | 21 | 2 | 19 | 163 | 224 | 398 | 2.76e-81 | 246 |
| MS.gene65757.t1 | MTR_7g046380 | 69.277 | 166 | 21 | 2 | 19 | 154 | 232 | 397 | 7.03e-74 | 226 |
| MS.gene65757.t1 | MTR_0057s0200 | 94.915 | 59 | 3 | 0 | 107 | 165 | 1 | 59 | 1.34e-36 | 121 |
| MS.gene65757.t1 | MTR_4g066320 | 56.881 | 109 | 42 | 2 | 62 | 165 | 13 | 121 | 1.32e-34 | 118 |
| MS.gene65757.t1 | MTR_0462s0020 | 30.726 | 179 | 88 | 4 | 15 | 161 | 117 | 291 | 2.34e-21 | 88.2 |
| MS.gene65757.t1 | MTR_3g028430 | 74.074 | 54 | 5 | 1 | 112 | 165 | 15 | 59 | 3.17e-20 | 79.3 |
| MS.gene65757.t1 | MTR_2g092960 | 27.119 | 177 | 94 | 3 | 20 | 165 | 1047 | 1219 | 1.31e-19 | 85.1 |
| MS.gene65757.t1 | MTR_8g017000 | 30.726 | 179 | 87 | 4 | 20 | 165 | 515 | 689 | 8.66e-19 | 82.8 |
| MS.gene65757.t1 | MTR_2g037270 | 30.168 | 179 | 88 | 4 | 20 | 165 | 515 | 689 | 2.78e-18 | 81.3 |
| MS.gene65757.t1 | MTR_8g051620 | 59.649 | 57 | 23 | 0 | 19 | 75 | 50 | 106 | 7.48e-18 | 74.7 |
| MS.gene65757.t1 | MTR_8g051620 | 59.649 | 57 | 23 | 0 | 19 | 75 | 50 | 106 | 1.01e-17 | 74.7 |
| MS.gene65757.t1 | MTR_4g048180 | 30.247 | 162 | 88 | 4 | 9 | 161 | 124 | 269 | 1.55e-15 | 72.0 |
| MS.gene65757.t1 | MTR_4g066300 | 88.235 | 34 | 4 | 0 | 103 | 136 | 5 | 38 | 5.82e-15 | 66.2 |
| MS.gene65757.t1 | MTR_2g086200 | 60.000 | 50 | 20 | 0 | 116 | 165 | 185 | 234 | 6.53e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGAAAGTGTTTGGAATTT+TGG | 0.087774 | 3.2:-7920696 | MS.gene65757:CDS |
| TTGCCATTAGGTTGCTTTCT+TGG | 0.248489 | 3.2:+7919792 | None:intergenic |
| GCAATAGAAAGAAGGTTAAA+TGG | 0.261318 | 3.2:-7920245 | MS.gene65757:CDS |
| ACGTAGAAGAACAATTATTA+AGG | 0.309665 | 3.2:+7920503 | None:intergenic |
| GGATTAAAATCCAAGCATAC+TGG | 0.319311 | 3.2:+7920671 | None:intergenic |
| TATAAGTCATCATCAGTTAA+TGG | 0.351177 | 3.2:+7919578 | None:intergenic |
| ACAAATAGATCGAAAGTGTT+TGG | 0.370084 | 3.2:-7920704 | MS.gene65757:CDS |
| TCTTGACTTGTATAAGGCTT+AGG | 0.381448 | 3.2:-7919525 | None:intergenic |
| TAATACTAAAACTAAAGTTC+AGG | 0.382008 | 3.2:-7920639 | MS.gene65757:CDS |
| TATCCACAATCATTGCCATT+AGG | 0.411955 | 3.2:+7919780 | None:intergenic |
| TATACTTACTTATATAGATA+TGG | 0.415179 | 3.2:+7920566 | None:intergenic |
| CTGCTAATATTACTAAGTCT+TGG | 0.472781 | 3.2:-7919725 | MS.gene65757:CDS |
| ATATGATTTGCGAAACCAAT+GGG | 0.486055 | 3.2:-7919558 | MS.gene65757:CDS |
| TAGCCAAGAAAGCAACCTAA+TGG | 0.495668 | 3.2:-7919795 | MS.gene65757:CDS |
| CAACCTAATGGCAATGATTG+TGG | 0.497368 | 3.2:-7919783 | MS.gene65757:CDS |
| TCATCATCAGTTAATGGCGT+TGG | 0.497730 | 3.2:+7919584 | None:intergenic |
| GGAGCAGTTACCCATGTTCA+AGG | 0.503945 | 3.2:-7921008 | MS.gene65757:CDS |
| CAAGAAAACAAGTTGCCCAT+TGG | 0.504204 | 3.2:+7919543 | None:intergenic |
| TGCTTAGGGCAATAGAAAGA+AGG | 0.512886 | 3.2:-7920253 | MS.gene65757:intron |
| AATACTAAAACTAAAGTTCA+GGG | 0.519185 | 3.2:-7920638 | MS.gene65757:CDS |
| TATTCGATTTCCTTGAACAT+GGG | 0.536637 | 3.2:+7920998 | None:intergenic |
| GATTAAAATCCAAGCATACT+GGG | 0.559851 | 3.2:+7920672 | None:intergenic |
| GTATTCGATTTCCTTGAACA+TGG | 0.576818 | 3.2:+7920997 | None:intergenic |
| TATATGATTTGCGAAACCAA+TGG | 0.577102 | 3.2:-7919559 | MS.gene65757:CDS |
| TAATATTACTAAGTCTTGGA+TGG | 0.604101 | 3.2:-7919721 | MS.gene65757:CDS |
| TTCAGGGACACATACAAACT+AGG | 0.610492 | 3.2:-7920622 | MS.gene65757:CDS |
| TATTACTAAGTCTTGGATGG+AGG | 0.643493 | 3.2:-7919718 | MS.gene65757:intron |
| AGCAATACTTGACACACACA+AGG | 0.680660 | 3.2:+7920975 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGATGATTTTTTTTAATT+TGG | - | chr3.2:7920231-7920250 | MS.gene65757:intron | 10.0% |
| !! | AAACTCAAATTATTTAGAAA+GGG | - | chr3.2:7920425-7920444 | MS.gene65757:intron | 15.0% |
| !! | AACTAATCACTTTATAAAAA+TGG | - | chr3.2:7920710-7920729 | MS.gene65757:CDS | 15.0% |
| !! | GTATTATACAAAAGAATTAT+TGG | + | chr3.2:7920618-7920637 | None:intergenic | 15.0% |
| !! | TAAACTCAAATTATTTAGAA+AGG | - | chr3.2:7920424-7920443 | MS.gene65757:intron | 15.0% |
| !! | TATACTTACTTATATAGATA+TGG | + | chr3.2:7919976-7919995 | None:intergenic | 15.0% |
| !!! | CTTTTATACTATTATCAATA+TGG | - | chr3.2:7919733-7919752 | MS.gene65757:CDS | 15.0% |
| !!! | TGTTAATTTTAAATTTGTCT+AGG | - | chr3.2:7919788-7919807 | MS.gene65757:CDS | 15.0% |
| !! | AATACTAAAACTAAAGTTCA+GGG | - | chr3.2:7919901-7919920 | MS.gene65757:intron | 20.0% |
| !! | TAAAGTAAAAAAATTCACTG+AGG | - | chr3.2:7920456-7920475 | MS.gene65757:intron | 20.0% |
| !! | TAATACAAGAAACAATCATA+CGG | + | chr3.2:7920377-7920396 | None:intergenic | 20.0% |
| !! | TAATACTAAAACTAAAGTTC+AGG | - | chr3.2:7919900-7919919 | MS.gene65757:intron | 20.0% |
| !!! | ATTGTTTCTTGTATTAAAAG+TGG | - | chr3.2:7920381-7920400 | MS.gene65757:intron | 20.0% |
| !!! | GTTTTAGTATTATGATCTTT+TGG | + | chr3.2:7919892-7919911 | None:intergenic | 20.0% |
| ! | AAATATACAGGGAACAATTA+AGG | + | chr3.2:7920353-7920372 | None:intergenic | 25.0% |
| ! | AACACTTAGATATTGAATTG+TGG | + | chr3.2:7919772-7919791 | None:intergenic | 25.0% |
| ! | AATCATACGGAAAATATACA+GGG | + | chr3.2:7920364-7920383 | None:intergenic | 25.0% |
| ! | ACGTAGAAGAACAATTATTA+AGG | + | chr3.2:7920039-7920058 | None:intergenic | 25.0% |
| ! | GTTAATAATTATTTGTCCCA+AGG | - | chr3.2:7920077-7920096 | MS.gene65757:intron | 25.0% |
| ! | TAATATTACTAAGTCTTGGA+TGG | - | chr3.2:7920818-7920837 | MS.gene65757:intron | 25.0% |
| ! | TATAAGTCATCATCAGTTAA+TGG | + | chr3.2:7920964-7920983 | None:intergenic | 25.0% |
| ! | TATATTAACCTACACATTGT+AGG | - | chr3.2:7920921-7920940 | MS.gene65757:intron | 25.0% |
| !! | TTGGTTAAGAAGTGTAAAAA+AGG | + | chr3.2:7920599-7920618 | None:intergenic | 25.0% |
| !!! | GTTATACATTTTAGTGTTGT+AGG | + | chr3.2:7920211-7920230 | None:intergenic | 25.0% |
| !!! | TTGTTTTCTTGACTTGTATA+AGG | - | chr3.2:7921008-7921027 | MS.gene65757:CDS | 25.0% |
| AACAAGAACCATACTTACTT+TGG | + | chr3.2:7920327-7920346 | None:intergenic | 30.0% | |
| ACAACAGTATCTATTCATGA+AGG | + | chr3.2:7920640-7920659 | None:intergenic | 30.0% | |
| ACAACTAAACTATTGTCCTT+GGG | + | chr3.2:7920096-7920115 | None:intergenic | 30.0% | |
| ACAAGAACCATACTTACTTT+GGG | + | chr3.2:7920326-7920345 | None:intergenic | 30.0% | |
| ATTATTTAGAAAGGGTAGTC+TGG | - | chr3.2:7920433-7920452 | MS.gene65757:intron | 30.0% | |
| CAATCATACGGAAAATATAC+AGG | + | chr3.2:7920365-7920384 | None:intergenic | 30.0% | |
| CTGCTAATATTACTAAGTCT+TGG | - | chr3.2:7920814-7920833 | MS.gene65757:intron | 30.0% | |
| GATTAAAATCCAAGCATACT+GGG | + | chr3.2:7919870-7919889 | None:intergenic | 30.0% | |
| GCAATAGAAAGAAGGTTAAA+TGG | - | chr3.2:7920294-7920313 | MS.gene65757:intron | 30.0% | |
| TATTCGATTTCCTTGAACAT+GGG | + | chr3.2:7919544-7919563 | None:intergenic | 30.0% | |
| TCATTGCTTCATTAAGATCT+TGG | + | chr3.2:7920134-7920153 | None:intergenic | 30.0% | |
| ! | ATATGATTTGCGAAACCAAT+GGG | - | chr3.2:7920981-7921000 | MS.gene65757:intron | 30.0% |
| ! | GTTAACATATAGAGCTTTAG+AGG | - | chr3.2:7920253-7920272 | MS.gene65757:intron | 30.0% |
| ! | TAGACTTAGCTTGATTACAT+TGG | - | chr3.2:7920483-7920502 | MS.gene65757:intron | 30.0% |
| ! | TATATGATTTGCGAAACCAA+TGG | - | chr3.2:7920980-7920999 | MS.gene65757:intron | 30.0% |
| !! | ACAAATAGATCGAAAGTGTT+TGG | - | chr3.2:7919835-7919854 | MS.gene65757:intron | 30.0% |
| !!! | ATCGAAAGTGTTTGGAATTT+TGG | - | chr3.2:7919843-7919862 | MS.gene65757:intron | 30.0% |
| ACACTTTGTAAGCACAACAA+TGG | + | chr3.2:7919668-7919687 | None:intergenic | 35.0% | |
| AGATCTAACTCTGTTCTGTA+TGG | + | chr3.2:7920519-7920538 | None:intergenic | 35.0% | |
| ATTGAAGACCTACAATGTGT+AGG | + | chr3.2:7920932-7920951 | None:intergenic | 35.0% | |
| CTTTCTTGGCTACAAATGAA+TGG | + | chr3.2:7920736-7920755 | None:intergenic | 35.0% | |
| GACAACTAAACTATTGTCCT+TGG | + | chr3.2:7920097-7920116 | None:intergenic | 35.0% | |
| GATCTAACTCTGTTCTGTAT+GGG | + | chr3.2:7920518-7920537 | None:intergenic | 35.0% | |
| GGATTAAAATCCAAGCATAC+TGG | + | chr3.2:7919871-7919890 | None:intergenic | 35.0% | |
| GTATTCGATTTCCTTGAACA+TGG | + | chr3.2:7919545-7919564 | None:intergenic | 35.0% | |
| TACAGAACAGAGTTAGATCT+AGG | - | chr3.2:7920519-7920538 | MS.gene65757:intron | 35.0% | |
| TATCCACAATCATTGCCATT+AGG | + | chr3.2:7920762-7920781 | None:intergenic | 35.0% | |
| TATTACTAAGTCTTGGATGG+AGG | - | chr3.2:7920821-7920840 | MS.gene65757:intron | 35.0% | |
| TGCTTCTGCTTATAAAAAGC+TGG | - | chr3.2:7920563-7920582 | MS.gene65757:intron | 35.0% | |
| TTTGTAAGCACAACAATGGT+GGG | + | chr3.2:7919664-7919683 | None:intergenic | 35.0% | |
| ! | AGATCTAGGATTCCATTACA+TGG | - | chr3.2:7920533-7920552 | MS.gene65757:intron | 35.0% |
| AAGCATGTGAGTCCATGTAA+TGG | + | chr3.2:7920548-7920567 | None:intergenic | 40.0% | |
| AGCAATACTTGACACACACA+AGG | + | chr3.2:7919567-7919586 | None:intergenic | 40.0% | |
| CAACCTAATGGCAATGATTG+TGG | - | chr3.2:7920756-7920775 | MS.gene65757:intron | 40.0% | |
| CAAGAAAACAAGTTGCCCAT+TGG | + | chr3.2:7920999-7921018 | None:intergenic | 40.0% | |
| CTTTGTAAGCACAACAATGG+TGG | + | chr3.2:7919665-7919684 | None:intergenic | 40.0% | |
| GTTTCGTCCCAAAGTAAGTA+TGG | - | chr3.2:7920316-7920335 | MS.gene65757:intron | 40.0% | |
| TAGCCAAGAAAGCAACCTAA+TGG | - | chr3.2:7920744-7920763 | MS.gene65757:intron | 40.0% | |
| TCATCATCAGTTAATGGCGT+TGG | + | chr3.2:7920958-7920977 | None:intergenic | 40.0% | |
| TGCTTAGGGCAATAGAAAGA+AGG | - | chr3.2:7920286-7920305 | MS.gene65757:intron | 40.0% | |
| TTCAGGGACACATACAAACT+AGG | - | chr3.2:7919917-7919936 | MS.gene65757:intron | 40.0% | |
| ! | AATGGTGGGACTCAAAGAAA+TGG | + | chr3.2:7919650-7919669 | None:intergenic | 40.0% |
| ! | AATTTTGGACCCAGTATGCT+TGG | - | chr3.2:7919858-7919877 | MS.gene65757:intron | 40.0% |
| ! | AGAGGTTCATCAACTTGCTT+AGG | - | chr3.2:7920271-7920290 | MS.gene65757:intron | 40.0% |
| ! | GAGGTTCATCAACTTGCTTA+GGG | - | chr3.2:7920272-7920291 | MS.gene65757:intron | 40.0% |
| ! | GATCTTGGTGCATTGAACAA+AGG | + | chr3.2:7920119-7920138 | None:intergenic | 40.0% |
| ! | TTGCCATTAGGTTGCTTTCT+TGG | + | chr3.2:7920750-7920769 | None:intergenic | 40.0% |
| !! | ATAAGTAATTAATATTAATA+AGG | + | chr3.2:7920904-7920923 | None:intergenic | 5.0% |
| GGAGCAGTTACCCATGTTCA+AGG | - | chr3.2:7919531-7919550 | MS.gene65757:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 7919526 | 7921035 | 7919526 | ID=MS.gene65757 |
| chr3.2 | mRNA | 7919526 | 7921035 | 7919526 | ID=MS.gene65757.t1;Parent=MS.gene65757 |
| chr3.2 | exon | 7920983 | 7921035 | 7920983 | ID=MS.gene65757.t1.exon1;Parent=MS.gene65757.t1 |
| chr3.2 | CDS | 7920983 | 7921035 | 7920983 | ID=cds.MS.gene65757.t1;Parent=MS.gene65757.t1 |
| chr3.2 | exon | 7920575 | 7920751 | 7920575 | ID=MS.gene65757.t1.exon2;Parent=MS.gene65757.t1 |
| chr3.2 | CDS | 7920575 | 7920751 | 7920575 | ID=cds.MS.gene65757.t1;Parent=MS.gene65757.t1 |
| chr3.2 | exon | 7920489 | 7920531 | 7920489 | ID=MS.gene65757.t1.exon3;Parent=MS.gene65757.t1 |
| chr3.2 | CDS | 7920489 | 7920531 | 7920489 | ID=cds.MS.gene65757.t1;Parent=MS.gene65757.t1 |
| chr3.2 | exon | 7920233 | 7920268 | 7920233 | ID=MS.gene65757.t1.exon4;Parent=MS.gene65757.t1 |
| chr3.2 | CDS | 7920233 | 7920268 | 7920233 | ID=cds.MS.gene65757.t1;Parent=MS.gene65757.t1 |
| chr3.2 | exon | 7919719 | 7919814 | 7919719 | ID=MS.gene65757.t1.exon5;Parent=MS.gene65757.t1 |
| chr3.2 | CDS | 7919719 | 7919814 | 7919719 | ID=cds.MS.gene65757.t1;Parent=MS.gene65757.t1 |
| chr3.2 | exon | 7919526 | 7919618 | 7919526 | ID=MS.gene65757.t1.exon6;Parent=MS.gene65757.t1 |
| chr3.2 | CDS | 7919526 | 7919618 | 7919526 | ID=cds.MS.gene65757.t1;Parent=MS.gene65757.t1 |
| Gene Sequence |
| Protein sequence |
