Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65819.t1 | XP_024635560.1 | 98.8 | 508 | 6 | 0 | 1 | 508 | 9 | 516 | 8.50E-294 | 1018.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65819.t1 | Q9M367 | 74.5 | 513 | 124 | 2 | 1 | 506 | 1 | 513 | 9.7e-222 | 770.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65819.t1 | A0A396IM07 | 98.8 | 508 | 6 | 0 | 1 | 508 | 9 | 516 | 6.1e-294 | 1018.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene65819 | MS.gene059541 | PPI |
| MS.gene65819 | MS.gene66405 | PPI |
| MS.gene65819 | MS.gene66413 | PPI |
| MS.gene65819 | MS.gene063319 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65819.t1 | MTR_3g018770 | 98.625 | 509 | 6 | 1 | 1 | 508 | 1 | 509 | 0.0 | 1053 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65819.t1 | AT3G61710 | 74.464 | 513 | 124 | 2 | 1 | 506 | 1 | 513 | 0.0 | 779 |
| MS.gene65819.t1 | AT3G61710 | 74.600 | 437 | 104 | 2 | 1 | 430 | 1 | 437 | 0.0 | 664 |
| MS.gene65819.t1 | AT3G61710 | 73.901 | 364 | 88 | 2 | 1 | 357 | 1 | 364 | 0.0 | 538 |
| MS.gene65819.t1 | AT3G61710 | 77.365 | 296 | 60 | 2 | 69 | 357 | 1 | 296 | 1.15e-155 | 446 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACGCATCTTACTGGAATTT+TGG | 0.125796 | 3.2:-9269839 | None:intergenic |
| AATATGTTATGTTCAGTTTC+TGG | 0.132096 | 3.2:-9273243 | None:intergenic |
| AAGAGGTTGAAGATGTAAAT+AGG | 0.219267 | 3.2:+9267915 | MS.gene65819:CDS |
| GGAGGAGAGAGATGCAATTT+TGG | 0.238592 | 3.2:+9269690 | MS.gene65819:intron |
| GACTCTTCCATTGTCTTTCC+AGG | 0.250506 | 3.2:-9267501 | None:intergenic |
| TTGGTCCAGTCAACTTGTTC+TGG | 0.268929 | 3.2:+9272518 | MS.gene65819:CDS |
| GCTTTAATAAGCAGGAAAAT+TGG | 0.278141 | 3.2:+9273057 | MS.gene65819:CDS |
| GATGAGAGAGACGACGAAAA+AGG | 0.286671 | 3.2:+9265791 | None:intergenic |
| ATTTCTCATGATGGAGAGTT+TGG | 0.302676 | 3.2:+9269784 | MS.gene65819:CDS |
| ATCTAGCCGCTTCAAAGAAT+TGG | 0.303262 | 3.2:+9269236 | MS.gene65819:CDS |
| TTTCCTGCTTATTAAAGCTT+TGG | 0.306193 | 3.2:-9273052 | None:intergenic |
| TGCAGAATTTGCCAAGTCTA+AGG | 0.309054 | 3.2:+9272594 | MS.gene65819:CDS |
| TGTAACAATGCTTACTTATA+AGG | 0.311546 | 3.2:-9272658 | None:intergenic |
| ATTTGAAATGGGCTCTCTAT+TGG | 0.316160 | 3.2:+9273108 | MS.gene65819:CDS |
| TGTGATGAGCACAACTAAAA+TGG | 0.323097 | 3.2:+9267398 | MS.gene65819:CDS |
| TTCCTGCTTATTAAAGCTTT+GGG | 0.325626 | 3.2:-9273051 | None:intergenic |
| ACACACTCTGTAATTTGAAA+TGG | 0.326673 | 3.2:+9273096 | MS.gene65819:CDS |
| TTTAAAACATTTAGGTAGAA+TGG | 0.328669 | 3.2:+9271192 | MS.gene65819:intron |
| TCAGTTTCTGGATTCAGATT+TGG | 0.333866 | 3.2:-9273231 | None:intergenic |
| TTCAACGATCCTTCTCGTTC+TGG | 0.342444 | 3.2:+9265921 | MS.gene65819:CDS |
| TGGGCTCTCTATTGGTTTGT+TGG | 0.349881 | 3.2:+9273116 | MS.gene65819:CDS |
| TGAGCCTGAGGTCTTTGCTT+TGG | 0.359710 | 3.2:-9267441 | None:intergenic |
| CGCAATCCACTTTGCATCGT+TGG | 0.360309 | 3.2:+9265873 | MS.gene65819:CDS |
| AAACTCTCCATCATGAGAAA+TGG | 0.363901 | 3.2:-9269782 | None:intergenic |
| GAAACTTCGGTCGGAAATAC+TGG | 0.365381 | 3.2:-9271268 | None:intergenic |
| TGGCTTCAGTACCACGCTTT+GGG | 0.368320 | 3.2:-9273211 | None:intergenic |
| GTTTCAATTGATTTCACATC+AGG | 0.393088 | 3.2:+9269494 | MS.gene65819:CDS |
| GCCTTTCCCATTTCTCATGA+TGG | 0.402011 | 3.2:+9269775 | MS.gene65819:CDS |
| TTCCATTGTCTTTCCAGGAT+GGG | 0.403861 | 3.2:-9267496 | None:intergenic |
| TTGGCTTCAGTACCACGCTT+TGG | 0.410296 | 3.2:-9273212 | None:intergenic |
| CGCATGCAGTCAAGGCATAA+AGG | 0.417474 | 3.2:-9267869 | None:intergenic |
| CTTCCATTGTCTTTCCAGGA+TGG | 0.419499 | 3.2:-9267497 | None:intergenic |
| CTTATTAAAGCTTTGGGTGA+TGG | 0.421070 | 3.2:-9273045 | None:intergenic |
| TTAAAACATTTAGGTAGAAT+GGG | 0.421462 | 3.2:+9271193 | MS.gene65819:intron |
| AACTCTCCATCATGAGAAAT+GGG | 0.423450 | 3.2:-9269781 | None:intergenic |
| GTCTGATAAGCTTGATAAAG+AGG | 0.427532 | 3.2:+9267898 | MS.gene65819:CDS |
| TGCTTGTGAAACTTCAATCT+TGG | 0.428703 | 3.2:-9269713 | None:intergenic |
| AGAGGTTGAAGATGTAAATA+GGG | 0.434838 | 3.2:+9267916 | MS.gene65819:CDS |
| CACACTCTGTAATTTGAAAT+GGG | 0.446991 | 3.2:+9273097 | MS.gene65819:CDS |
| GGGTCGGAATCGGAGCTTAC+CGG | 0.447487 | 3.2:+9265812 | MS.gene65819:CDS |
| CAAACCCATCGAGGGACGTT+CGG | 0.451867 | 3.2:-9265840 | None:intergenic |
| AATCTGCGTCTGATGGAGGC+GGG | 0.455975 | 3.2:+9267547 | MS.gene65819:CDS |
| CAGCTCATAAGTACTGTTGC+TGG | 0.456802 | 3.2:-9272016 | None:intergenic |
| CACCCAAAGCTTTAATAAGC+AGG | 0.463428 | 3.2:+9273049 | MS.gene65819:CDS |
| CACACTGCTAGCACCATGAA+TGG | 0.472744 | 3.2:-9267379 | None:intergenic |
| GAATCTGCGTCTGATGGAGG+CGG | 0.475727 | 3.2:+9267546 | MS.gene65819:CDS |
| CAATCTCAAATGCTCGAGTC+AGG | 0.481760 | 3.2:-9267650 | None:intergenic |
| TGAAGCCTGTCTTAAACGCT+TGG | 0.485526 | 3.2:+9267952 | MS.gene65819:CDS |
| TTGCTGGTGTCTGTGATTCG+AGG | 0.493541 | 3.2:-9272000 | None:intergenic |
| CCCCTCCAAGCGTTTAAGAC+AGG | 0.494529 | 3.2:-9267957 | None:intergenic |
| GGTAGCTTGAAACATTTCTC+TGG | 0.496750 | 3.2:-9272637 | None:intergenic |
| TAAATGTGTGTAGGGATGCA+AGG | 0.500342 | 3.2:+9267351 | MS.gene65819:intron |
| ATCTGCGTCTGATGGAGGCG+GGG | 0.501367 | 3.2:+9267548 | MS.gene65819:CDS |
| TGGATCCGAACGTCCCTCGA+TGG | 0.506017 | 3.2:+9265835 | MS.gene65819:CDS |
| AAGAAGGCAAGCTTGGCCCC+AGG | 0.506324 | 3.2:-9271231 | None:intergenic |
| GGGGAAACTCATTGTCTCCG+GGG | 0.509988 | 3.2:+9267568 | MS.gene65819:CDS |
| TCTGCGTCTGATGGAGGCGG+GGG | 0.514763 | 3.2:+9267549 | MS.gene65819:CDS |
| TATGCAGAAGAAGGCAAGCT+TGG | 0.514890 | 3.2:-9271238 | None:intergenic |
| GTTCTCTTGATCCTTAGACT+TGG | 0.518682 | 3.2:-9272605 | None:intergenic |
| CATTTGCCACCTCATAACTC+TGG | 0.520812 | 3.2:+9267606 | MS.gene65819:CDS |
| GGGATGAGATAAATGCTGCC+TGG | 0.524441 | 3.2:+9271213 | MS.gene65819:CDS |
| CGTGGTGATACATCCCATCC+TGG | 0.530828 | 3.2:+9267483 | MS.gene65819:CDS |
| AAGTCGGAATCTGCGTCTGA+TGG | 0.536084 | 3.2:+9267540 | MS.gene65819:CDS |
| GGATGAGATAAATGCTGCCT+GGG | 0.539884 | 3.2:+9271214 | MS.gene65819:CDS |
| GTGCTCCAGAACAAGTTGAC+TGG | 0.540441 | 3.2:-9272523 | None:intergenic |
| TAGCCGCTTCAAAGAATTGG+AGG | 0.541238 | 3.2:+9269239 | MS.gene65819:CDS |
| AGCCTGTCTTAAACGCTTGG+AGG | 0.542097 | 3.2:+9267955 | MS.gene65819:CDS |
| GGATCCGAACGTCCCTCGAT+GGG | 0.545053 | 3.2:+9265836 | MS.gene65819:CDS |
| GCCTGTCTTAAACGCTTGGA+GGG | 0.547231 | 3.2:+9267956 | MS.gene65819:CDS |
| ATGCAAGGGTCATCCATTCA+TGG | 0.547282 | 3.2:+9267366 | MS.gene65819:CDS |
| ATACCATTGCTGAAAGAGGT+TGG | 0.550854 | 3.2:-9273151 | None:intergenic |
| CTTCCAACCTCTTTCAGCAA+TGG | 0.551764 | 3.2:+9273148 | MS.gene65819:CDS |
| AGAAGAAAGAAGACTAGAAG+CGG | 0.552430 | 3.2:+9269149 | MS.gene65819:CDS |
| AAATGTGTGTAGGGATGCAA+GGG | 0.554122 | 3.2:+9267352 | MS.gene65819:intron |
| CGAGGGACGTTCGGATCCAC+CGG | 0.556220 | 3.2:-9265831 | None:intergenic |
| ATTCGAGGGTAGCTACCCAT+TGG | 0.561039 | 3.2:-9271985 | None:intergenic |
| GGCACATTGTATGCAGAAGA+AGG | 0.565635 | 3.2:-9271247 | None:intergenic |
| CTGTCTTAAACGCTTGGAGG+GGG | 0.566042 | 3.2:+9267958 | MS.gene65819:CDS |
| GATGTATCACCACGAGGCCG+AGG | 0.567728 | 3.2:-9267474 | None:intergenic |
| AAGCAAAGACCTCAGGCTCA+AGG | 0.574216 | 3.2:+9267444 | MS.gene65819:CDS |
| GCTCCTCCAATTCTTTGAAG+CGG | 0.574855 | 3.2:-9269242 | None:intergenic |
| TCGACGCCAACGATGCAAAG+TGG | 0.576527 | 3.2:-9265879 | None:intergenic |
| GTTGCCAAAGCAAAGACCTC+AGG | 0.579073 | 3.2:+9267437 | MS.gene65819:CDS |
| AGGCTCAAGGAGTTCCTCCT+CGG | 0.586199 | 3.2:+9267457 | MS.gene65819:CDS |
| TGCTGGTGTCTGTGATTCGA+GGG | 0.588115 | 3.2:-9271999 | None:intergenic |
| GAATTGAATCCAGAGTTATG+AGG | 0.590276 | 3.2:-9267615 | None:intergenic |
| ATCCCATCCTGGAAAGACAA+TGG | 0.590592 | 3.2:+9267494 | MS.gene65819:CDS |
| AGCTTGAAACATTTCTCTGG+TGG | 0.591769 | 3.2:-9272634 | None:intergenic |
| TCGGAATCTGCGTCTGATGG+AGG | 0.592390 | 3.2:+9267543 | MS.gene65819:CDS |
| GGGTACAAAGATCCTACAGC+TGG | 0.595630 | 3.2:-9273191 | None:intergenic |
| CCTGTCTTAAACGCTTGGAG+GGG | 0.597661 | 3.2:+9267957 | MS.gene65819:CDS |
| GGAGGAACTCCTTGAGCCTG+AGG | 0.598305 | 3.2:-9267453 | None:intergenic |
| CATGCTGAAGTGCCAGCTGT+AGG | 0.598389 | 3.2:+9273179 | MS.gene65819:CDS |
| ACAAAACTCGCATGCAGTCA+AGG | 0.604059 | 3.2:-9267877 | None:intergenic |
| GGAGTTCCTCCTCGGCCTCG+TGG | 0.607193 | 3.2:+9267465 | MS.gene65819:CDS |
| TGAAGTAAATGCTGAACTGA+AGG | 0.613224 | 3.2:+9269200 | MS.gene65819:CDS |
| AAGGATACTGAAACTTCGGT+CGG | 0.619914 | 3.2:-9271277 | None:intergenic |
| TGACCACCATGATCAACCCC+CGG | 0.629604 | 3.2:-9267585 | None:intergenic |
| GCGCACCTGTGTCTGTGTAG+TGG | 0.630167 | 3.2:-9267673 | None:intergenic |
| CAAATTCTGCAAAATCCTTG+AGG | 0.636799 | 3.2:-9272582 | None:intergenic |
| TCCATCATGAGAAATGGGAA+AGG | 0.638413 | 3.2:-9269776 | None:intergenic |
| CAACGATGCAAAGTGGATTG+CGG | 0.640313 | 3.2:-9265872 | None:intergenic |
| GGGAAACTCATTGTCTCCGG+GGG | 0.643396 | 3.2:+9267569 | MS.gene65819:CDS |
| AGTTCTGACAAACCCATCGA+GGG | 0.647106 | 3.2:-9265848 | None:intergenic |
| GAAGATACCATTGCTGAAAG+AGG | 0.647747 | 3.2:-9273155 | None:intergenic |
| AGAAACTAACCAGAACGAGA+AGG | 0.653735 | 3.2:-9265930 | None:intergenic |
| GTATCACCACGAGGCCGAGG+AGG | 0.654136 | 3.2:-9267471 | None:intergenic |
| TCGGAATCGGAGCTTACCGG+TGG | 0.658622 | 3.2:+9265815 | MS.gene65819:CDS |
| TTGAATCCAGAGTTATGAGG+TGG | 0.665081 | 3.2:-9267612 | None:intergenic |
| CAGTTCTGACAAACCCATCG+AGG | 0.678531 | 3.2:-9265849 | None:intergenic |
| GATTGCCACTACACAGACAC+AGG | 0.687132 | 3.2:+9267668 | MS.gene65819:CDS |
| TAAGAAAATCAGCCTCGCTG+AGG | 0.688641 | 3.2:-9267994 | None:intergenic |
| GGATCTTTGTACCCAAAGCG+TGG | 0.690117 | 3.2:+9273200 | MS.gene65819:CDS |
| GCTTCAAAGAATTGGAGGAG+CGG | 0.703613 | 3.2:+9269244 | MS.gene65819:CDS |
| GGCTAAAGATGTCCTCAGCG+AGG | 0.710980 | 3.2:+9267982 | MS.gene65819:CDS |
| GATGAGATAAATGCTGCCTG+GGG | 0.771422 | 3.2:+9271215 | MS.gene65819:CDS |
| GGATGGGATGTATCACCACG+AGG | 0.784660 | 3.2:-9267480 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 9265792 | 9273253 | 9265792 | ID=MS.gene65819 |
| chr3.2 | mRNA | 9265792 | 9273253 | 9265792 | ID=MS.gene65819.t1;Parent=MS.gene65819 |
| chr3.2 | exon | 9265792 | 9265942 | 9265792 | ID=MS.gene65819.t1.exon1;Parent=MS.gene65819.t1 |
| chr3.2 | CDS | 9265792 | 9265942 | 9265792 | ID=cds.MS.gene65819.t1;Parent=MS.gene65819.t1 |
| chr3.2 | exon | 9267364 | 9267689 | 9267364 | ID=MS.gene65819.t1.exon2;Parent=MS.gene65819.t1 |
| chr3.2 | CDS | 9267364 | 9267689 | 9267364 | ID=cds.MS.gene65819.t1;Parent=MS.gene65819.t1 |
| chr3.2 | exon | 9267860 | 9268030 | 9267860 | ID=MS.gene65819.t1.exon3;Parent=MS.gene65819.t1 |
| chr3.2 | CDS | 9267860 | 9268030 | 9267860 | ID=cds.MS.gene65819.t1;Parent=MS.gene65819.t1 |
| chr3.2 | exon | 9269141 | 9269265 | 9269141 | ID=MS.gene65819.t1.exon4;Parent=MS.gene65819.t1 |
| chr3.2 | CDS | 9269141 | 9269265 | 9269141 | ID=cds.MS.gene65819.t1;Parent=MS.gene65819.t1 |
| chr3.2 | exon | 9269467 | 9269515 | 9269467 | ID=MS.gene65819.t1.exon5;Parent=MS.gene65819.t1 |
| chr3.2 | CDS | 9269467 | 9269515 | 9269467 | ID=cds.MS.gene65819.t1;Parent=MS.gene65819.t1 |
| chr3.2 | exon | 9269691 | 9269849 | 9269691 | ID=MS.gene65819.t1.exon6;Parent=MS.gene65819.t1 |
| chr3.2 | CDS | 9269691 | 9269849 | 9269691 | ID=cds.MS.gene65819.t1;Parent=MS.gene65819.t1 |
| chr3.2 | exon | 9271206 | 9271291 | 9271206 | ID=MS.gene65819.t1.exon7;Parent=MS.gene65819.t1 |
| chr3.2 | CDS | 9271206 | 9271291 | 9271206 | ID=cds.MS.gene65819.t1;Parent=MS.gene65819.t1 |
| chr3.2 | exon | 9271966 | 9272037 | 9271966 | ID=MS.gene65819.t1.exon8;Parent=MS.gene65819.t1 |
| chr3.2 | CDS | 9271966 | 9272037 | 9271966 | ID=cds.MS.gene65819.t1;Parent=MS.gene65819.t1 |
| chr3.2 | exon | 9272516 | 9272665 | 9272516 | ID=MS.gene65819.t1.exon9;Parent=MS.gene65819.t1 |
| chr3.2 | CDS | 9272516 | 9272665 | 9272516 | ID=cds.MS.gene65819.t1;Parent=MS.gene65819.t1 |
| chr3.2 | exon | 9273016 | 9273253 | 9273016 | ID=MS.gene65819.t1.exon10;Parent=MS.gene65819.t1 |
| chr3.2 | CDS | 9273016 | 9273253 | 9273016 | ID=cds.MS.gene65819.t1;Parent=MS.gene65819.t1 |
| Gene Sequence |
| Protein sequence |
