AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66837

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66837.t1 MTR_3g116130 99.286 280 2 0 1 280 1 280 0.0 558
MS.gene66837.t1 MTR_3g116130 99.288 281 2 0 1 281 1 281 0.0 553
MS.gene66837.t1 MTR_8g024520 55.500 200 73 9 23 214 19 210 1.62e-55 181
MS.gene66837.t1 MTR_8g024520 55.500 200 73 9 23 214 19 210 1.71e-55 181
MS.gene66837.t1 MTR_5g064120 57.895 171 44 3 21 177 8 164 3.52e-49 164
MS.gene66837.t1 MTR_4g035310 78.947 95 20 0 19 113 6 100 6.80e-49 163
MS.gene66837.t1 MTR_4g035310 78.947 95 20 0 19 113 6 100 8.68e-49 163
MS.gene66837.t1 MTR_3g092490 76.543 81 19 0 24 104 21 101 2.64e-43 146
MS.gene66837.t1 MTR_3g092490 76.543 81 19 0 24 104 21 101 5.90e-42 145
MS.gene66837.t1 MTR_3g092490 76.543 81 19 0 24 104 21 101 6.32e-42 144
MS.gene66837.t1 MTR_7g117495 67.778 90 29 0 21 110 10 99 1.90e-40 141
MS.gene66837.t1 MTR_7g117495 67.778 90 29 0 21 110 10 99 2.00e-40 140
MS.gene66837.t1 MTR_2g026180 38.261 115 50 3 30 129 5 113 5.89e-20 89.7
MS.gene66837.t1 MTR_4g088010 53.012 83 39 0 25 107 72 154 7.12e-20 88.6
MS.gene66837.t1 MTR_4g088010 29.286 140 86 3 24 150 158 297 6.08e-11 62.4
MS.gene66837.t1 MTR_8g463890 46.988 83 44 0 25 107 69 151 2.53e-18 84.7
MS.gene66837.t1 MTR_7g088140 44.048 84 47 0 24 107 122 205 9.30e-18 83.2
MS.gene66837.t1 MTR_7g088140 44.048 84 47 0 24 107 122 205 1.72e-17 82.4
MS.gene66837.t1 MTR_3g055990 42.667 75 42 1 30 104 7 80 2.28e-17 82.0
MS.gene66837.t1 MTR_3g055990 42.667 75 42 1 30 104 7 80 2.58e-17 82.0
MS.gene66837.t1 MTR_5g088220 40.964 83 49 0 25 107 161 243 8.69e-17 80.5
MS.gene66837.t1 MTR_1g073690 40.000 80 47 1 25 104 2 80 1.86e-16 79.3
MS.gene66837.t1 MTR_1g073690 37.037 81 51 0 24 104 107 187 3.76e-11 63.5
MS.gene66837.t1 MTR_4g070190 44.737 76 41 1 30 104 9 84 7.34e-16 73.9
MS.gene66837.t1 MTR_3g080130 36.190 105 65 2 11 114 18 121 2.88e-15 71.2
MS.gene66837.t1 MTR_5g097580 41.333 75 43 1 30 104 7 80 4.74e-15 75.1
MS.gene66837.t1 MTR_5g097580 30.508 177 94 5 30 178 107 282 2.29e-11 64.3
MS.gene66837.t1 MTR_4g070140 44.737 76 41 1 30 104 9 84 5.58e-15 72.0
MS.gene66837.t1 MTR_5g097580 41.333 75 43 1 30 104 7 80 6.20e-15 74.7
MS.gene66837.t1 MTR_5g097580 30.508 177 94 5 30 178 107 282 2.94e-11 63.9
MS.gene66837.t1 MTR_4g070080 40.964 83 47 2 23 104 1 82 1.99e-14 68.9
MS.gene66837.t1 MTR_3g025880 40.594 101 57 3 11 110 27 125 4.60e-14 72.0
MS.gene66837.t1 MTR_7g013730 40.244 82 47 2 30 110 41 121 4.73e-14 70.5
MS.gene66837.t1 MTR_3g084040 42.029 69 40 0 29 97 36 104 4.76e-14 68.6
MS.gene66837.t1 MTR_4g070080 40.964 83 47 2 23 104 1 82 4.81e-14 68.9
MS.gene66837.t1 MTR_3g084040 42.424 66 38 0 29 94 36 101 5.78e-14 67.0
MS.gene66837.t1 MTR_4g109540 40.964 83 47 2 29 110 42 123 7.14e-14 70.5
MS.gene66837.t1 MTR_4g063560 45.070 71 38 1 29 98 33 103 2.24e-13 65.5
MS.gene66837.t1 MTR_7g105260 38.462 91 53 2 30 117 33 123 9.52e-13 64.7
MS.gene66837.t1 MTR_7g013740 34.426 122 67 3 1 110 1 121 1.72e-12 64.3
MS.gene66837.t1 MTR_3g103420 35.000 80 51 1 25 104 6 84 1.14e-11 64.7
MS.gene66837.t1 MTR_4g109540 37.363 91 47 3 29 110 42 131 1.40e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66837.t1 AT1G76460 68.512 289 73 8 1 279 1 281 2.15e-129 369
MS.gene66837.t1 AT1G20880 65.000 280 74 8 13 281 8 274 3.07e-112 325
MS.gene66837.t1 AT1G20880 65.000 280 74 8 13 281 8 274 3.07e-112 325
MS.gene66837.t1 AT1G20880 65.000 280 74 8 13 281 8 274 3.07e-112 325
MS.gene66837.t1 AT1G22330 58.385 161 51 4 21 176 9 158 8.00e-51 169
MS.gene66837.t1 AT1G33470 43.089 246 115 5 25 251 3 242 8.80e-51 167
MS.gene66837.t1 AT1G33470 43.320 247 114 6 25 251 3 243 8.89e-50 165
MS.gene66837.t1 AT1G33470 43.802 242 110 6 25 246 3 238 1.50e-49 164
MS.gene66837.t1 AT1G78260 81.176 85 16 0 21 105 9 93 2.46e-46 157
MS.gene66837.t1 AT1G22910 78.022 91 20 0 23 113 7 97 2.99e-46 155
MS.gene66837.t1 AT2G46780 51.832 191 80 6 20 199 13 202 3.53e-46 158
MS.gene66837.t1 AT2G46780 51.832 191 80 6 20 199 13 202 3.53e-46 158
MS.gene66837.t1 AT1G78260 81.176 85 16 0 21 105 9 93 3.96e-46 156
MS.gene66837.t1 AT1G22910 78.022 91 20 0 23 113 7 97 8.22e-46 155
MS.gene66837.t1 AT1G22910 78.022 91 20 0 23 113 7 97 1.57e-45 157
MS.gene66837.t1 AT3G54770 65.625 96 32 1 19 113 7 102 4.47e-38 135
MS.gene66837.t1 AT3G54770 65.625 96 32 1 19 113 7 102 5.33e-38 135
MS.gene66837.t1 AT5G11412 66.250 80 27 0 25 104 48 127 1.34e-34 123
MS.gene66837.t1 AT5G53680 55.000 80 36 0 25 104 9 88 1.96e-28 107
MS.gene66837.t1 AT3G06970 55.422 83 37 0 25 107 8 90 5.11e-26 104
MS.gene66837.t1 AT3G54770 55.556 90 25 2 45 128 1 81 6.26e-25 99.8
MS.gene66837.t1 AT5G53720 55.263 76 34 0 29 104 9 84 3.97e-24 94.0
MS.gene66837.t1 AT2G22090 46.988 83 44 0 25 107 100 182 6.93e-19 85.5
MS.gene66837.t1 AT2G22090 46.988 83 44 0 25 107 100 182 7.06e-19 85.5
MS.gene66837.t1 AT4G39260 46.053 76 40 1 30 104 7 82 1.74e-18 79.0
MS.gene66837.t1 AT4G39260 46.053 76 40 1 30 104 7 82 2.04e-18 79.7
MS.gene66837.t1 AT4G39260 46.053 76 40 1 30 104 7 82 5.78e-18 79.7
MS.gene66837.t1 AT3G15010 48.193 83 43 0 25 107 71 153 6.26e-18 83.2
MS.gene66837.t1 AT3G15010 33.333 102 68 0 12 113 150 251 1.91e-14 73.2
MS.gene66837.t1 AT3G15010 48.193 83 43 0 25 107 71 153 6.26e-18 83.2
MS.gene66837.t1 AT3G15010 33.333 102 68 0 12 113 150 251 1.91e-14 73.2
MS.gene66837.t1 AT4G26650 42.667 75 42 1 30 104 16 89 1.07e-17 82.8
MS.gene66837.t1 AT4G26650 42.667 75 42 1 30 104 13 86 1.10e-17 82.8
MS.gene66837.t1 AT5G47620 38.889 90 51 2 29 118 6 91 1.89e-17 82.0
MS.gene66837.t1 AT5G47620 38.889 90 51 2 29 118 6 91 1.89e-17 82.0
MS.gene66837.t1 AT5G47620 38.889 90 51 2 29 118 6 91 1.89e-17 82.0
MS.gene66837.t1 AT4G39260 52.632 57 27 0 30 86 7 63 2.12e-17 76.3
MS.gene66837.t1 AT5G47620 46.377 69 36 1 29 97 6 73 2.18e-17 82.0
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.35e-17 81.6
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.35e-17 81.6
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.35e-17 81.6
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.35e-17 81.6
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.35e-17 81.6
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.44e-17 81.6
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.47e-17 81.6
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.47e-17 81.6
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.47e-17 81.6
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.47e-17 81.6
MS.gene66837.t1 AT5G55550 45.588 68 36 1 30 97 7 73 3.48e-17 81.6
MS.gene66837.t1 AT2G19380 31.073 177 107 5 1 166 382 554 6.67e-17 80.9
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT3G56860 40.964 83 49 0 25 107 136 218 1.93e-16 79.3
MS.gene66837.t1 AT2G41060 38.889 90 55 0 25 114 124 213 2.07e-16 79.3
MS.gene66837.t1 AT2G41060 38.889 90 55 0 25 114 124 213 2.07e-16 79.3
MS.gene66837.t1 AT2G41060 38.889 90 55 0 25 114 124 213 2.07e-16 79.3
MS.gene66837.t1 AT3G23830 39.326 89 52 2 29 116 35 122 2.71e-16 74.3
MS.gene66837.t1 AT3G23830 39.326 89 52 2 29 116 35 122 2.71e-16 74.3
MS.gene66837.t1 AT2G22100 29.500 200 126 6 10 195 140 338 2.86e-16 78.6
MS.gene66837.t1 AT4G13850 45.833 72 35 1 29 100 35 102 2.89e-16 73.9
MS.gene66837.t1 AT3G07810 41.333 75 43 1 30 104 7 80 4.26e-16 78.2
MS.gene66837.t1 AT4G13850 43.529 85 46 2 29 112 35 118 4.36e-16 73.9
MS.gene66837.t1 AT3G07810 41.333 75 43 1 30 104 7 80 4.51e-16 78.2
MS.gene66837.t1 AT4G13850 43.529 85 46 2 29 112 35 118 5.38e-16 73.9
MS.gene66837.t1 AT4G13850 43.529 85 46 2 29 112 35 118 6.02e-16 73.9
MS.gene66837.t1 AT2G21660 42.683 82 45 2 24 104 4 84 2.61e-15 72.4
MS.gene66837.t1 AT1G17640 34.545 110 59 3 30 139 67 163 3.72e-15 75.1
MS.gene66837.t1 AT1G17640 34.545 110 59 3 30 139 67 163 3.72e-15 75.1
MS.gene66837.t1 AT1G17640 34.545 110 59 3 30 139 67 163 3.72e-15 75.1
MS.gene66837.t1 AT2G21660 42.683 82 45 2 24 104 4 84 3.74e-15 72.4
MS.gene66837.t1 AT2G33410 33.913 115 63 2 30 132 7 120 1.27e-14 73.6
MS.gene66837.t1 AT4G14300 38.750 80 48 1 25 104 2 80 2.92e-14 72.8
MS.gene66837.t1 AT4G14300 38.750 80 48 1 25 104 2 80 2.92e-14 72.8
MS.gene66837.t1 AT5G61030 38.750 80 39 2 29 108 40 109 1.54e-12 67.0
MS.gene66837.t1 AT5G61030 38.750 80 39 2 29 108 40 109 1.54e-12 67.0
MS.gene66837.t1 AT1G74230 37.179 78 49 0 29 106 34 111 4.50e-12 65.5
MS.gene66837.t1 AT3G26420 43.478 69 38 1 30 97 8 76 4.81e-12 64.7
MS.gene66837.t1 AT1G18630 42.857 70 39 1 29 97 36 105 1.70e-11 61.6
MS.gene66837.t1 AT5G59865 48.980 49 25 0 29 77 26 74 3.13e-11 59.7
MS.gene66837.t1 AT1G22910 71.795 39 11 0 75 113 8 46 4.95e-11 61.2
MS.gene66837.t1 AT3G13224 32.877 73 48 1 30 102 20 91 5.73e-11 62.8
MS.gene66837.t1 AT5G04280 44.444 54 30 0 29 82 7 60 7.00e-11 62.0

Find 108 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCGTTACTTTGAACAATT+TGG 0.153087 3.4:-99055055 MS.gene66837:CDS
AATACAACTATTTATCCTTA+TGG 0.237313 3.4:-99053513 MS.gene66837:CDS
GGAATGGTTTATCCTCCTTA+TGG 0.259852 3.4:-99054254 MS.gene66837:CDS
AGGACTGTACCGCTAGGATA+TGG 0.287341 3.4:+99053351 None:intergenic
CAATTGTTGAATTCTCCTTT+TGG 0.309482 3.4:-99055142 MS.gene66837:CDS
TATTTATCCTTATGGACAAT+TGG 0.319044 3.4:-99053505 MS.gene66837:CDS
ACCTGTGGGTATACGTATTC+AGG 0.345135 3.4:+99053690 None:intergenic
GGTGCCTACGTTGGAGGTTA+TGG 0.353929 3.4:-99054311 MS.gene66837:CDS
GTCCTGTTATTGATGGTAGA+AGG 0.354869 3.4:-99054598 MS.gene66837:CDS
CAACAGTACCTTCAGATATA+TGG 0.362755 3.4:-99053552 MS.gene66837:CDS
GAATGGTTTATCCTCCTTAT+GGG 0.365347 3.4:-99054253 MS.gene66837:intron
GGAATTGCTGCACCAGTTCC+AGG 0.368536 3.4:-99052274 MS.gene66837:CDS
CAGGATCAAGTTCGAGTTCT+GGG 0.370870 3.4:-99055168 MS.gene66837:CDS
GCATACCAGGGTCCAAGTTC+AGG 0.377967 3.4:-99055187 MS.gene66837:CDS
TCAGGATCAAGTTCGAGTTC+TGG 0.380866 3.4:-99055169 MS.gene66837:CDS
TACTGTTGTTGACCAGCATA+AGG 0.402296 3.4:+99053567 None:intergenic
CTCGAACTTGATCCTGAACT+TGG 0.405699 3.4:+99055175 None:intergenic
ACTTTCCTATGGCTATCAAC+AGG 0.406701 3.4:-99054276 MS.gene66837:CDS
GCCCTTCTACCATCAATAAC+AGG 0.411876 3.4:+99054596 None:intergenic
CAATTGGGTCAAGCTGTTCC+TGG 0.414666 3.4:-99053489 MS.gene66837:CDS
ATTTATCCTTATGGACAATT+GGG 0.419932 3.4:-99053504 MS.gene66837:CDS
GCTCACAAAACCGTAGCCTT+TGG 0.426083 3.4:+99054985 None:intergenic
ATAGCATACCATATGAGATT+GGG 0.427085 3.4:+99054534 None:intergenic
CAGGCTCTCCTAGCAGCCTC+TGG 0.436509 3.4:+99054632 None:intergenic
TAAAGTCTTCGTTGGTGGAT+TGG 0.444418 3.4:-99055104 MS.gene66837:CDS
AATAGCATACCATATGAGAT+TGG 0.458747 3.4:+99054533 None:intergenic
ACCGTAGCCTTTGGATCGTC+CGG 0.458930 3.4:+99054994 None:intergenic
CTACCATCAATAACAGGACT+TGG 0.459160 3.4:+99054602 None:intergenic
CCTCAACCATCTGTTTGGTC+AGG 0.464509 3.4:+99052205 None:intergenic
CGTCCCCTCAACCATCTGTT+TGG 0.471470 3.4:+99052200 None:intergenic
GCCCGTGGTGCCTACGTTGG+AGG 0.473342 3.4:-99054317 MS.gene66837:CDS
ACAATAAATTGTGGTTGGCC+TGG 0.475736 3.4:+99052256 None:intergenic
TCATGGTTATACAGCAATGC+AGG 0.476637 3.4:-99053463 MS.gene66837:CDS
GCTTGACCCAATTGTCCATA+AGG 0.479896 3.4:+99053498 None:intergenic
CATCAGATTGTGCCCTATGG+TGG 0.479906 3.4:-99053423 MS.gene66837:CDS
CTATGGCTATCAACAGGGAA+TGG 0.482459 3.4:-99054270 MS.gene66837:CDS
CAAGCTGTTCCTGGCGGTCA+TGG 0.484640 3.4:-99053480 MS.gene66837:CDS
GATCCAAGTCCTGTTATTGA+TGG 0.488968 3.4:-99054605 MS.gene66837:CDS
ACCTCCAACGTAGGCACCAC+GGG 0.492680 3.4:+99054316 None:intergenic
CTTCCAACATAAGGAGATGC+TGG 0.500325 3.4:+99054350 None:intergenic
TATACTAAAGTCTTCGTTGG+TGG 0.503912 3.4:-99055109 MS.gene66837:CDS
GGACTGTACCGCTAGGATAT+GGG 0.505345 3.4:+99053352 None:intergenic
ACTTATACTAAAGTCTTCGT+TGG 0.505982 3.4:-99055112 MS.gene66837:CDS
TAGAAGGGCTAATTGTAACT+TGG 0.507997 3.4:-99054582 MS.gene66837:CDS
AATTGTGGTTGGCCTGGAAC+TGG 0.508286 3.4:+99052262 None:intergenic
CAGCATCCCACTCACCCATA+AGG 0.512059 3.4:+99054239 None:intergenic
TACCAACAACCACTTTCCTA+TGG 0.512893 3.4:-99054287 MS.gene66837:CDS
ACTGTTGTTGACCAGCATAA+GGG 0.516296 3.4:+99053568 None:intergenic
CTTCAGATATATGGTGTGCC+TGG 0.520918 3.4:-99053543 MS.gene66837:CDS
GGTTGCAAACTTCCAACATA+AGG 0.521111 3.4:+99054341 None:intergenic
ATTAACATTGGACCCACCAT+AGG 0.522838 3.4:+99053410 None:intergenic
AATTGTAACTTGGCTTCGCA+TGG 0.524644 3.4:-99054572 MS.gene66837:CDS
CCAACGTAGGCACCACGGGC+TGG 0.528290 3.4:+99054320 None:intergenic
AGTTGTTATTGCATTAACAT+TGG 0.528485 3.4:+99053398 None:intergenic
GGTCATCAGATTGTGCCCTA+TGG 0.532673 3.4:-99053426 MS.gene66837:CDS
TCTTCGTTGGTGGATTGGCA+TGG 0.533106 3.4:-99055099 MS.gene66837:CDS
CTCCAAATTGTTCAAAGTAA+CGG 0.539239 3.4:+99055053 None:intergenic
CTTCGTTGGTGGATTGGCAT+GGG 0.539784 3.4:-99055098 MS.gene66837:CDS
GCTGTATAACCATGACCGCC+AGG 0.540975 3.4:+99053471 None:intergenic
TCCTAGCAGCCTCTGGATCA+CGG 0.541344 3.4:+99054639 None:intergenic
ATCAGATTGTGCCCTATGGT+GGG 0.545371 3.4:-99053422 MS.gene66837:CDS
AGACCAGCATCTCCTTATGT+TGG 0.552478 3.4:-99054353 MS.gene66837:CDS
TCCTGTTATTGATGGTAGAA+GGG 0.553892 3.4:-99054597 MS.gene66837:CDS
GGTATGTACAACCCTTATGC+TGG 0.554007 3.4:-99053579 MS.gene66837:CDS
TGGGACTTGAATGGCAGGCA+CGG 0.554141 3.4:+99053371 None:intergenic
GGGACTTGAATGGCAGGCAC+GGG 0.554491 3.4:+99053372 None:intergenic
CCAGCCCGTGGTGCCTACGT+TGG 0.554812 3.4:-99054320 MS.gene66837:CDS
AGTGTGATTAATGGCATACC+AGG 0.559750 3.4:-99055200 None:intergenic
TTGATCCTGAACTTGGACCC+TGG 0.560791 3.4:+99055182 None:intergenic
AGCCATAGGAAAGTGGTTGT+TGG 0.564090 3.4:+99054285 None:intergenic
GGATATGGGACTTGAATGGC+AGG 0.565965 3.4:+99053366 None:intergenic
CTGACCAAACAGATGGTTGA+GGG 0.567593 3.4:-99052204 None:intergenic
CCTCAGTTTATGCAAGGTAG+CGG 0.569657 3.4:-99052229 MS.gene66837:CDS
GAGAAACAATAAATTGTGGT+TGG 0.575026 3.4:+99052251 None:intergenic
GTATAAGTAGTATCTCCAAA+AGG 0.576903 3.4:+99055127 None:intergenic
ATGCAGGGTTATACAGTACC+AGG 0.577248 3.4:-99053447 MS.gene66837:CDS
CATTCCCTGTTGATAGCCAT+AGG 0.583457 3.4:+99054271 None:intergenic
TAGCATACCATATGAGATTG+GGG 0.583743 3.4:+99054535 None:intergenic
GTTTCTCCTCAGTTTATGCA+AGG 0.585322 3.4:-99052235 MS.gene66837:CDS
TCCGTGATCCAGAGGCTGCT+AGG 0.585544 3.4:-99054640 MS.gene66837:CDS
GCTAGGATATGGGACTTGAA+TGG 0.586705 3.4:+99053362 None:intergenic
AACAGGACTTGGATCAGCAC+AGG 0.593305 3.4:+99054613 None:intergenic
TGTTGATAGCCATAGGAAAG+TGG 0.600019 3.4:+99054278 None:intergenic
AAGAACACCGGACGATCCAA+AGG 0.601945 3.4:-99055001 MS.gene66837:CDS
CCTGACCAAACAGATGGTTG+AGG 0.601994 3.4:-99052205 None:intergenic
CTTTCCTATGGCTATCAACA+GGG 0.608429 3.4:-99054275 MS.gene66837:CDS
TAGGGCACAATCTGATGACC+TGG 0.609886 3.4:+99053429 None:intergenic
ACCGGACGATCCAAAGGCTA+CGG 0.610204 3.4:-99054995 MS.gene66837:CDS
TGAGGAGAAACAATAAATTG+TGG 0.616646 3.4:+99052247 None:intergenic
TTAACATTGGACCCACCATA+GGG 0.618343 3.4:+99053411 None:intergenic
AACCTCCAACGTAGGCACCA+CGG 0.618711 3.4:+99054315 None:intergenic
GGAAGTTTGCAACCAGCCCG+TGG 0.632845 3.4:-99054332 MS.gene66837:CDS
CAGGCACACCATATATCTGA+AGG 0.636375 3.4:+99053544 None:intergenic
GGTGACTTTCCGTGATCCAG+AGG 0.637483 3.4:-99054648 MS.gene66837:CDS
AGCGGTCCTGACCAAACAGA+TGG 0.639591 3.4:-99052211 MS.gene66837:CDS
GCTTCGCATGGTCGTCCACG+TGG 0.643033 3.4:-99054560 MS.gene66837:CDS
ATAGTTGTATTCATCGCACC+AGG 0.645261 3.4:+99053525 None:intergenic
CATGGTTATACAGCAATGCA+GGG 0.646571 3.4:-99053462 MS.gene66837:CDS
CATCCCACTCACCCATAAGG+AGG 0.654523 3.4:+99054242 None:intergenic
ACCTGAATACGTATACCCAC+AGG 0.654631 3.4:-99053691 MS.gene66837:intron
GTAGCCATAACCTCCAACGT+AGG 0.685657 3.4:+99054307 None:intergenic
GTCATCACTGATAAGAACAC+CGG 0.699829 3.4:-99055013 MS.gene66837:CDS
CCGCTACCTTGCATAAACTG+AGG 0.704628 3.4:+99052229 None:intergenic
TTGGGTCAAGCTGTTCCTGG+CGG 0.707255 3.4:-99053486 MS.gene66837:CDS
GTGTGATTAATGGCATACCA+GGG 0.710100 3.4:-99055199 None:intergenic
AGCATACCATATGAGATTGG+GGG 0.719081 3.4:+99054536 None:intergenic
GTTCTTATCAGTGATGACGA+CGG 0.732189 3.4:+99055018 None:intergenic
ATTCAAGTCCCATATCCTAG+CGG 0.771653 3.4:-99053360 MS.gene66837:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTTAATTAAAAAATAT+AGG - chr3.4:99054922-99054941 MS.gene66837:intron 0.0%
!! AAAAATAAAATATAAATTCC+CGG + chr3.4:99054608-99054627 None:intergenic 10.0%
!! AAATTTATGTACAAAATTAA+GGG - chr3.4:99054872-99054891 MS.gene66837:intron 10.0%
!! TAAATTTATGTACAAAATTA+AGG - chr3.4:99054871-99054890 MS.gene66837:intron 10.0%
!! TGATAAATTATTTAATGTAA+CGG + chr3.4:99054228-99054247 None:intergenic 10.0%
!!! TAATTTTGTACATAAATTTA+GGG + chr3.4:99054871-99054890 None:intergenic 10.0%
!!! TATCAGTTTTTTTTTTTATT+TGG - chr3.4:99053732-99053751 MS.gene66837:intron 10.0%
!!! TCTAGTAAATTTTTTTTTTT+TGG + chr3.4:99054485-99054504 None:intergenic 10.0%
!!! TTAATTTTGTACATAAATTT+AGG + chr3.4:99054872-99054891 None:intergenic 10.0%
!! AAAAAAAATTTACTAGAACA+TGG - chr3.4:99054488-99054507 MS.gene66837:intron 15.0%
!! AAAAAAATTTACTAGAACAT+GGG - chr3.4:99054489-99054508 MS.gene66837:intron 15.0%
!! AAAATAAAATATAAATTCCC+GGG + chr3.4:99054607-99054626 None:intergenic 15.0%
!! AATGTTAATTCCAATATATA+TGG + chr3.4:99054706-99054725 None:intergenic 15.0%
!! ATATAAACAAAGAAAGAAAT+AGG + chr3.4:99052489-99052508 None:intergenic 15.0%
!! ATGTTAATTCCAATATATAT+GGG + chr3.4:99054705-99054724 None:intergenic 15.0%
!! TATAAACAAAGAAAGAAATA+GGG + chr3.4:99052488-99052507 None:intergenic 15.0%
!! TGTTTATATTCTATCATATA+AGG - chr3.4:99052500-99052519 MS.gene66837:intron 15.0%
!!! ATAATGATAATTTTATTCCT+AGG - chr3.4:99054136-99054155 MS.gene66837:intron 15.0%
!!! TAAATTTTTTTTTTTTGGTC+AGG + chr3.4:99054480-99054499 None:intergenic 15.0%
!!! TTTTTTTTTTTATTTGGCTT+AGG - chr3.4:99053738-99053757 MS.gene66837:intron 15.0%
!! AATACAACTATTTATCCTTA+TGG - chr3.4:99053884-99053903 MS.gene66837:intron 20.0%
!! TAAATAATTTATCAGAACGT+GGG - chr3.4:99054234-99054253 MS.gene66837:intron 20.0%
!! TATTATTATTAGCAGATTGA+CGG + chr3.4:99053225-99053244 None:intergenic 20.0%
!! TTAAATAATTTATCAGAACG+TGG - chr3.4:99054233-99054252 MS.gene66837:intron 20.0%
!! TTATTTACTATAGAATGACT+TGG - chr3.4:99052692-99052711 MS.gene66837:intron 20.0%
!!! AATTATCATTATTGTTTTCC+TGG + chr3.4:99054128-99054147 None:intergenic 20.0%
!!! ATTATCATTATTGTTTTCCT+GGG + chr3.4:99054127-99054146 None:intergenic 20.0%
!!! GATCTTTTCAACATTATATT+TGG - chr3.4:99053651-99053670 MS.gene66837:intron 20.0%
!!! GATTTCTTCTTATCTTTTAT+AGG - chr3.4:99053014-99053033 MS.gene66837:intron 20.0%
!!! GTTTTGTAATATATGATAGA+AGG - chr3.4:99054894-99054913 MS.gene66837:intron 20.0%
! AAACAAAAGCCCATATATAT+TGG - chr3.4:99054693-99054712 MS.gene66837:intron 25.0%
! AAATAATTTATCAGAACGTG+GGG - chr3.4:99054235-99054254 MS.gene66837:intron 25.0%
! AAGCAATGTATATTCTATGT+AGG + chr3.4:99052985-99053004 None:intergenic 25.0%
! AATTTGAGAAAATGATGACA+TGG - chr3.4:99052563-99052582 MS.gene66837:intron 25.0%
! ACAATGCATTATTATATGCA+GGG - chr3.4:99054388-99054407 MS.gene66837:intron 25.0%
! ACATGGAGACTAATATTAAA+TGG + chr3.4:99053560-99053579 None:intergenic 25.0%
! ACTTTAAATTCTGTGCAAAT+AGG - chr3.4:99055074-99055093 MS.gene66837:CDS 25.0%
! ATTTATCCTTATGGACAATT+GGG - chr3.4:99053893-99053912 MS.gene66837:intron 25.0%
! GTAATATATGATAGAAGGTT+GGG - chr3.4:99054899-99054918 MS.gene66837:intron 25.0%
! TACTTGATTAGTAGTTAGTT+AGG - chr3.4:99053394-99053413 MS.gene66837:CDS 25.0%
! TATTTATCCTTATGGACAAT+TGG - chr3.4:99053892-99053911 MS.gene66837:intron 25.0%
! TGTAATATATGATAGAAGGT+TGG - chr3.4:99054898-99054917 MS.gene66837:intron 25.0%
! TTATTACATCCAATTGACAT+AGG + chr3.4:99053529-99053548 None:intergenic 25.0%
!! AGTTGTTATTGCATTAACAT+TGG + chr3.4:99054002-99054021 None:intergenic 25.0%
!! ATTTAATATTAGTCTCCATG+TGG - chr3.4:99053559-99053578 MS.gene66837:CDS 25.0%
!! CATTTCTTTGAAAAAATCCT+AGG + chr3.4:99054156-99054175 None:intergenic 25.0%
!! TACAATGTTCAACAATGTTT+TGG + chr3.4:99052544-99052563 None:intergenic 25.0%
!! TCTTCTTATCTTTTATAGGA+CGG - chr3.4:99053018-99053037 MS.gene66837:intron 25.0%
!! TTTAATATTAGTCTCCATGT+GGG - chr3.4:99053560-99053579 MS.gene66837:CDS 25.0%
!!! AATTCTTAACAAGCATTGAT+TGG - chr3.4:99054756-99054775 MS.gene66837:intron 25.0%
!!! CTAGTCATTTTTATTCACAT+GGG + chr3.4:99054335-99054354 None:intergenic 25.0%
!!! GTTTTATCTTTTCAGGTATA+CGG - chr3.4:99053676-99053695 MS.gene66837:intron 25.0%
!!! TCTAGTCATTTTTATTCACA+TGG + chr3.4:99054336-99054355 None:intergenic 25.0%
!!! TTCGTAAGTATTTGATTTTC+AGG - chr3.4:99055102-99055121 MS.gene66837:CDS 25.0%
!!! TTTGGTTGTTTTATCTTTTC+AGG - chr3.4:99053669-99053688 MS.gene66837:intron 25.0%
AAAAAAACTGATATGACCTG+TGG + chr3.4:99053725-99053744 None:intergenic 30.0%
AAAAAACTGATATGACCTGT+GGG + chr3.4:99053724-99053743 None:intergenic 30.0%
AATAGCATACCATATGAGAT+TGG + chr3.4:99052867-99052886 None:intergenic 30.0%
ACCTTATAAGGTGAATAAGT+GGG + chr3.4:99054437-99054456 None:intergenic 30.0%
ACTTATACTAAAGTCTTCGT+TGG - chr3.4:99052285-99052304 MS.gene66837:intron 30.0%
AGTCAAAAAAACACGATAAC+TGG + chr3.4:99053322-99053341 None:intergenic 30.0%
ATAGCATACCATATGAGATT+GGG + chr3.4:99052866-99052885 None:intergenic 30.0%
ATGAATGATACGACATATGT+AGG - chr3.4:99054828-99054847 MS.gene66837:intron 30.0%
ATGGCTGTTCATAAACAAAA+AGG + chr3.4:99054652-99054671 None:intergenic 30.0%
CAATGCATTATTATATGCAG+GGG - chr3.4:99054389-99054408 MS.gene66837:intron 30.0%
CTATCTTATATGCATGGAAT+TGG - chr3.4:99052718-99052737 MS.gene66837:intron 30.0%
CTCCAAATTGTTCAAAGTAA+CGG + chr3.4:99052347-99052366 None:intergenic 30.0%
CTTAAGGTCTATCAATGTAA+TGG + chr3.4:99054181-99054200 None:intergenic 30.0%
CTTGCAAGAATATGTTGTAT+AGG + chr3.4:99053374-99053393 None:intergenic 30.0%
GAAAATTCTATTACCAGGAA+TGG + chr3.4:99054302-99054321 None:intergenic 30.0%
GACAATGCATTATTATATGC+AGG - chr3.4:99054387-99054406 MS.gene66837:intron 30.0%
GAGAAACAATAAATTGTGGT+TGG + chr3.4:99055149-99055168 None:intergenic 30.0%
GGAACAAAAATAATATGCCT+AGG + chr3.4:99054543-99054562 None:intergenic 30.0%
GTATAAGTAGTATCTCCAAA+AGG + chr3.4:99052273-99052292 None:intergenic 30.0%
GTCAAAAAAACACGATAACT+GGG + chr3.4:99053321-99053340 None:intergenic 30.0%
TATGGAAAACCTATGTCAAT+TGG - chr3.4:99053517-99053536 MS.gene66837:CDS 30.0%
TGAGGAGAAACAATAAATTG+TGG + chr3.4:99055153-99055172 None:intergenic 30.0%
TGGCTGTTCATAAACAAAAA+GGG + chr3.4:99054651-99054670 None:intergenic 30.0%
TGGTTTCTATCTTATATGCA+TGG - chr3.4:99052712-99052731 MS.gene66837:intron 30.0%
TGTAACTGCTAAAATAAGCA+TGG + chr3.4:99053284-99053303 None:intergenic 30.0%
! ATTTTTATTCACATGGGAGA+GGG + chr3.4:99054329-99054348 None:intergenic 30.0%
! CAATGCTTGTTAAGAATTGA+AGG + chr3.4:99054754-99054773 None:intergenic 30.0%
! CAATTGTTGAATTCTCCTTT+TGG - chr3.4:99052255-99052274 MS.gene66837:CDS 30.0%
! CTTCTTATCTTTTATAGGAC+GGG - chr3.4:99053019-99053038 MS.gene66837:intron 30.0%
! TAATTGAAGTTCTGATCAAC+AGG - chr3.4:99054945-99054964 MS.gene66837:intron 30.0%
!!! AGTTATCGTGTTTTTTTGAC+TGG - chr3.4:99053321-99053340 MS.gene66837:intron 30.0%
!!! TTCCTTTTTTGTGAGTAATG+AGG + chr3.4:99054204-99054223 None:intergenic 30.0%
AAAGAACCATAACACAGGAA+AGG + chr3.4:99053257-99053276 None:intergenic 35.0%
AAGAATTTCCACTTGTGACT+CGG - chr3.4:99054079-99054098 MS.gene66837:intron 35.0%
AGCCTCATTACTCACAAAAA+AGG - chr3.4:99054199-99054218 MS.gene66837:intron 35.0%
ATATAAATTCCCGGGAATAG+AGG + chr3.4:99054599-99054618 None:intergenic 35.0%
CAAAGAATATCCACCAAAGA+TGG + chr3.4:99054671-99054690 None:intergenic 35.0%
CAACAGTACCTTCAGATATA+TGG - chr3.4:99053845-99053864 MS.gene66837:intron 35.0%
CACAAGTGGAAATTCTTTCT+TGG + chr3.4:99054076-99054095 None:intergenic 35.0%
CACCTTATAAGGTGAATAAG+TGG + chr3.4:99054438-99054457 None:intergenic 35.0%
CCTAGGAATAAATAATACCG+AGG + chr3.4:99054526-99054545 None:intergenic 35.0%
CCTCGGTATTATTTATTCCT+AGG - chr3.4:99054523-99054542 MS.gene66837:intron 35.0%
CCTTATAAGGTGAATAAGTG+GGG + chr3.4:99054436-99054455 None:intergenic 35.0%
CTTATATGCATGGAATTGGA+GGG - chr3.4:99052722-99052741 MS.gene66837:intron 35.0%
GAAAAAGGAATTCCTGTGTT+TGG + chr3.4:99054572-99054591 None:intergenic 35.0%
GAATGGTTTATCCTCCTTAT+GGG - chr3.4:99053144-99053163 MS.gene66837:intron 35.0%
GGAAAGTTATTCCAAAGGAA+TGG + chr3.4:99054106-99054125 None:intergenic 35.0%
GGAATCACATATCTATGTGT+CGG - chr3.4:99052584-99052603 MS.gene66837:intron 35.0%
TAGAAGGGCTAATTGTAACT+TGG - chr3.4:99052815-99052834 MS.gene66837:intron 35.0%
TAGCATACCATATGAGATTG+GGG + chr3.4:99052865-99052884 None:intergenic 35.0%
TATACTAAAGTCTTCGTTGG+TGG - chr3.4:99052288-99052307 MS.gene66837:intron 35.0%
TCTTATATGCATGGAATTGG+AGG - chr3.4:99052721-99052740 MS.gene66837:intron 35.0%
TGGCTAGAATTCACCTTATA+AGG + chr3.4:99054449-99054468 None:intergenic 35.0%
TGTTTATGAACAGCCATCTT+TGG - chr3.4:99054655-99054674 MS.gene66837:intron 35.0%
TTGCACACTATTGCAAAAAC+TGG - chr3.4:99053181-99053200 MS.gene66837:intron 35.0%
TTTGTGAGTAATGAGGCTTA+AGG + chr3.4:99054197-99054216 None:intergenic 35.0%
! AGTAGCTAGTCTGTAAAGAA+TGG - chr3.4:99054970-99054989 MS.gene66837:intron 35.0%
! CATAGGTTTTCCATATACAG+TGG + chr3.4:99053512-99053531 None:intergenic 35.0%
! CATTTTTATTCACATGGGAG+AGG + chr3.4:99054330-99054349 None:intergenic 35.0%
! TCCTGTTATTGATGGTAGAA+GGG - chr3.4:99052800-99052819 MS.gene66837:intron 35.0%
! TTTTTATTCACATGGGAGAG+GGG + chr3.4:99054328-99054347 None:intergenic 35.0%
!! TCTATGTAGGAAGAGTATTC+TGG + chr3.4:99052972-99052991 None:intergenic 35.0%
AACTTCTACCGTGTCCAATA+GGG - chr3.4:99053797-99053816 MS.gene66837:intron 40.0%
AAGGAATTCCTGTGTTTGGT+AGG + chr3.4:99054568-99054587 None:intergenic 40.0%
AAGGGTTGTACATACCCTAT+TGG + chr3.4:99053814-99053833 None:intergenic 40.0%
AATCACTGGTCCACTGTATA+TGG - chr3.4:99053499-99053518 MS.gene66837:CDS 40.0%
AATTCCTGTGTTTGGTAGGA+AGG + chr3.4:99054564-99054583 None:intergenic 40.0%
AATTGTAACTTGGCTTCGCA+TGG - chr3.4:99052825-99052844 MS.gene66837:intron 40.0%
ACCAGTGATTACCAAATCTC+GGG + chr3.4:99053430-99053449 None:intergenic 40.0%
ACTGGTATATGTCAGTGATG+AGG - chr3.4:99053339-99053358 MS.gene66837:intron 40.0%
ACTGTTGTTGACCAGCATAA+GGG + chr3.4:99053832-99053851 None:intergenic 40.0%
ACTTTCCTATGGCTATCAAC+AGG - chr3.4:99053121-99053140 MS.gene66837:intron 40.0%
AGAATTTCCACTTGTGACTC+GGG - chr3.4:99054080-99054099 MS.gene66837:intron 40.0%
AGCATACCATATGAGATTGG+GGG + chr3.4:99052864-99052883 None:intergenic 40.0%
ATAGTTGTATTCATCGCACC+AGG + chr3.4:99053875-99053894 None:intergenic 40.0%
ATTAACATTGGACCCACCAT+AGG + chr3.4:99053990-99054009 None:intergenic 40.0%
ATTCAAGTCCCATATCCTAG+CGG - chr3.4:99054037-99054056 MS.gene66837:intron 40.0%
CACATACACTTATCAGCTGT+TGG - chr3.4:99054785-99054804 MS.gene66837:intron 40.0%
CATGGTTATACAGCAATGCA+GGG - chr3.4:99053935-99053954 MS.gene66837:intron 40.0%
CCATGCTAGTATGATCAACA+CGG + chr3.4:99052662-99052681 None:intergenic 40.0%
CCCCACTTATTCACCTTATA+AGG - chr3.4:99054433-99054452 MS.gene66837:intron 40.0%
CTACCATCAATAACAGGACT+TGG + chr3.4:99052798-99052817 None:intergenic 40.0%
CTTTCCTATGGCTATCAACA+GGG - chr3.4:99053122-99053141 MS.gene66837:intron 40.0%
GACACAAAGAACCATAACAC+AGG + chr3.4:99053262-99053281 None:intergenic 40.0%
GACCAGTGATTACCAAATCT+CGG + chr3.4:99053431-99053450 None:intergenic 40.0%
GAGATGAAAATCCCGAGATT+TGG - chr3.4:99053416-99053435 MS.gene66837:CDS 40.0%
GATCCAAGTCCTGTTATTGA+TGG - chr3.4:99052792-99052811 MS.gene66837:intron 40.0%
GCTGATAAGTGTATGTGATC+TGG + chr3.4:99054782-99054801 None:intergenic 40.0%
GGAAATTCTTTCTTGGACAG+AGG + chr3.4:99054069-99054088 None:intergenic 40.0%
GGAATGGTTTATCCTCCTTA+TGG - chr3.4:99053143-99053162 MS.gene66837:intron 40.0%
GGTGGGAAAATTCTATTACC+AGG + chr3.4:99054307-99054326 None:intergenic 40.0%
GGTTGCAAACTTCCAACATA+AGG + chr3.4:99053059-99053078 None:intergenic 40.0%
GTCATCACTGATAAGAACAC+CGG - chr3.4:99052384-99052403 MS.gene66837:intron 40.0%
GTTTCTCCTCAGTTTATGCA+AGG - chr3.4:99055162-99055181 MS.gene66837:CDS 40.0%
TAAGGTGAATTCTAGCCACT+AGG - chr3.4:99054451-99054470 MS.gene66837:intron 40.0%
TACCAACAACCACTTTCCTA+TGG - chr3.4:99053110-99053129 MS.gene66837:intron 40.0%
TACTGTTGTTGACCAGCATA+AGG + chr3.4:99053833-99053852 None:intergenic 40.0%
TCATGGTTATACAGCAATGC+AGG - chr3.4:99053934-99053953 MS.gene66837:intron 40.0%
TCCTGGGAAAGTTATTCCAA+AGG + chr3.4:99054111-99054130 None:intergenic 40.0%
TGTACATACCCTATTGGACA+CGG + chr3.4:99053808-99053827 None:intergenic 40.0%
TTAACATTGGACCCACCATA+GGG + chr3.4:99053989-99054008 None:intergenic 40.0%
TTAGATGCACAATGCAGATG+TGG + chr3.4:99053600-99053619 None:intergenic 40.0%
TTATGAACAGCCATCTTTGG+TGG - chr3.4:99054658-99054677 MS.gene66837:intron 40.0%
! AAGCCTTATCTCACTAAGTG+GGG - chr3.4:99053473-99053492 MS.gene66837:CDS 40.0%
! ACAATAAATTGTGGTTGGCC+TGG + chr3.4:99055144-99055163 None:intergenic 40.0%
! CAAGCCTTATCTCACTAAGT+GGG - chr3.4:99053472-99053491 MS.gene66837:CDS 40.0%
! CATGGGTTAAGTAGATTCCT+CGG - chr3.4:99054506-99054525 MS.gene66837:intron 40.0%
! CGCCGTTACTTTGAACAATT+TGG - chr3.4:99052342-99052361 MS.gene66837:intron 40.0%
! GGTTGTTGTTGTTGTTGTAG+TGG + chr3.4:99053453-99053472 None:intergenic 40.0%
! GTCCTGTTATTGATGGTAGA+AGG - chr3.4:99052799-99052818 MS.gene66837:intron 40.0%
! GTTCTTATCAGTGATGACGA+CGG + chr3.4:99052382-99052401 None:intergenic 40.0%
! TAAAGTCTTCGTTGGTGGAT+TGG - chr3.4:99052293-99052312 MS.gene66837:intron 40.0%
! TATTAGTCTCCATGTGGGTA+AGG - chr3.4:99053565-99053584 MS.gene66837:CDS 40.0%
! TTTTTCCCATCCTCTATTCC+CGG - chr3.4:99054586-99054605 MS.gene66837:CDS 40.0%
!! AATGAGTTTTGCTCTGAACG+TGG - chr3.4:99054271-99054290 MS.gene66837:CDS 40.0%
!! ATGAGTTTTGCTCTGAACGT+GGG - chr3.4:99054272-99054291 MS.gene66837:CDS 40.0%
!! TCACCTCTAAAGCATGATTC+TGG + chr3.4:99053633-99053652 None:intergenic 40.0%
!! TCCTTTGGAATAACTTTCCC+AGG - chr3.4:99054107-99054126 MS.gene66837:intron 40.0%
!! TGTTGATAGCCATAGGAAAG+TGG + chr3.4:99053122-99053141 None:intergenic 40.0%
!!! CTTTTCAGGTATACGGCATA+TGG - chr3.4:99053683-99053702 MS.gene66837:intron 40.0%
AAATTCCCGGGAATAGAGGA+TGG + chr3.4:99054595-99054614 None:intergenic 45.0%
AATTCCCGGGAATAGAGGAT+GGG + chr3.4:99054594-99054613 None:intergenic 45.0%
ACCTGAATACGTATACCCAC+AGG - chr3.4:99053706-99053725 MS.gene66837:CDS 45.0%
ACCTGTGGGTATACGTATTC+AGG + chr3.4:99053710-99053729 None:intergenic 45.0%
ACGCCAGAATCATGCTTTAG+AGG - chr3.4:99053627-99053646 MS.gene66837:intron 45.0%
AGACCAGCATCTCCTTATGT+TGG - chr3.4:99053044-99053063 MS.gene66837:intron 45.0%
AGCCATAGGAAAGTGGTTGT+TGG + chr3.4:99053115-99053134 None:intergenic 45.0%
AGGTGTGTCAAGTGTCCAAT+AGG + chr3.4:99052612-99052631 None:intergenic 45.0%
ATCAGATTGTGCCCTATGGT+GGG - chr3.4:99053975-99053994 MS.gene66837:intron 45.0%
ATCTATGTGTCGGACCCTAT+TGG - chr3.4:99052594-99052613 MS.gene66837:intron 45.0%
ATGCAGGGTTATACAGTACC+AGG - chr3.4:99053950-99053969 MS.gene66837:intron 45.0%
CAACTTCTACCGTGTCCAAT+AGG - chr3.4:99053796-99053815 MS.gene66837:intron 45.0%
CAGGCACACCATATATCTGA+AGG + chr3.4:99053856-99053875 None:intergenic 45.0%
CATTATTATATGCAGGGGCC+GGG - chr3.4:99054394-99054413 MS.gene66837:intron 45.0%
CCACTTAGTGAGATAAGGCT+TGG + chr3.4:99053474-99053493 None:intergenic 45.0%
CCTCAGTTTATGCAAGGTAG+CGG - chr3.4:99055168-99055187 MS.gene66837:CDS 45.0%
CTATGGCTATCAACAGGGAA+TGG - chr3.4:99053127-99053146 MS.gene66837:intron 45.0%
CTCGAACTTGATCCTGAACT+TGG + chr3.4:99052225-99052244 None:intergenic 45.0%
CTCTTGCCTTTCCTGTGTTA+TGG - chr3.4:99053248-99053267 MS.gene66837:intron 45.0%
CTTCAGATATATGGTGTGCC+TGG - chr3.4:99053854-99053873 MS.gene66837:intron 45.0%
CTTCCAACATAAGGAGATGC+TGG + chr3.4:99053050-99053069 None:intergenic 45.0%
GCATTATTATATGCAGGGGC+CGG - chr3.4:99054393-99054412 MS.gene66837:intron 45.0%
GCCCTTCTACCATCAATAAC+AGG + chr3.4:99052804-99052823 None:intergenic 45.0%
GCTAGGATATGGGACTTGAA+TGG + chr3.4:99054038-99054057 None:intergenic 45.0%
GCTTGACCCAATTGTCCATA+AGG + chr3.4:99053902-99053921 None:intergenic 45.0%
GGACACGGTAGAAGTTGAAA+AGG + chr3.4:99053793-99053812 None:intergenic 45.0%
GGGAATAGAGGATGGGAAAA+AGG + chr3.4:99054587-99054606 None:intergenic 45.0%
GGTATGTACAACCCTTATGC+TGG - chr3.4:99053818-99053837 MS.gene66837:intron 45.0%
GGTGTGTCAAGTGTCCAATA+GGG + chr3.4:99052611-99052630 None:intergenic 45.0%
TATCCTCCTTATGGGTGAGT+GGG - chr3.4:99053152-99053171 MS.gene66837:intron 45.0%
TGCACAATGCAGATGTGGTT+AGG + chr3.4:99053595-99053614 None:intergenic 45.0%
TGCTAGTATGATCAACACGG+TGG + chr3.4:99052659-99052678 None:intergenic 45.0%
TGTTCCTTCCTACCAAACAC+AGG - chr3.4:99054557-99054576 MS.gene66837:CDS 45.0%
TTAGGAACACCTTACCCACA+TGG + chr3.4:99053577-99053596 None:intergenic 45.0%
TTATCCTCCTTATGGGTGAG+TGG - chr3.4:99053151-99053170 MS.gene66837:intron 45.0%
! CAGGATCAAGTTCGAGTTCT+GGG - chr3.4:99052229-99052248 MS.gene66837:CDS 45.0%
! CCAAGCCTTATCTCACTAAG+TGG - chr3.4:99053471-99053490 MS.gene66837:CDS 45.0%
! CCGTGTTGATCATACTAGCA+TGG - chr3.4:99052659-99052678 MS.gene66837:intron 45.0%
! TCAGGATCAAGTTCGAGTTC+TGG - chr3.4:99052228-99052247 MS.gene66837:CDS 45.0%
! TCCCGAGATTTGGTAATCAC+TGG - chr3.4:99053426-99053445 MS.gene66837:CDS 45.0%
! TCTGATCAGATCAGAAGTGC+CGG - chr3.4:99052631-99052650 MS.gene66837:intron 45.0%
! TGCATGGAATTGGAGGGTAT+AGG - chr3.4:99052728-99052747 MS.gene66837:intron 45.0%
! TTTTCCCATCCTCTATTCCC+GGG - chr3.4:99054587-99054606 MS.gene66837:CDS 45.0%
!! CATTCCCTGTTGATAGCCAT+AGG + chr3.4:99053129-99053148 None:intergenic 45.0%
!! TTTTTGGTCAGGTAGCCTAG+TGG + chr3.4:99054469-99054488 None:intergenic 45.0%
AACAGGACTTGGATCAGCAC+AGG + chr3.4:99052787-99052806 None:intergenic 50.0%
AAGAACACCGGACGATCCAA+AGG - chr3.4:99052396-99052415 MS.gene66837:intron 50.0%
ACCAACTGAGCTATGCTCAC+AGG + chr3.4:99054365-99054384 None:intergenic 50.0%
ACGCCCCACTTAGTGAGATA+AGG + chr3.4:99053479-99053498 None:intergenic 50.0%
ACTAAGTGGGGCGTAATCAC+TGG - chr3.4:99053485-99053504 MS.gene66837:CDS 50.0%
AGGACTGTACCGCTAGGATA+TGG + chr3.4:99054049-99054068 None:intergenic 50.0%
CAATTGGGTCAAGCTGTTCC+TGG - chr3.4:99053908-99053927 MS.gene66837:intron 50.0%
CATCAGATTGTGCCCTATGG+TGG - chr3.4:99053974-99053993 MS.gene66837:intron 50.0%
CCGCTACCTTGCATAAACTG+AGG + chr3.4:99055171-99055190 None:intergenic 50.0%
GCTCACAAAACCGTAGCCTT+TGG + chr3.4:99052415-99052434 None:intergenic 50.0%
GGACTGTACCGCTAGGATAT+GGG + chr3.4:99054048-99054067 None:intergenic 50.0%
GGATATGGGACTTGAATGGC+AGG + chr3.4:99054034-99054053 None:intergenic 50.0%
GGCACTTCTGATCTGATCAG+AGG + chr3.4:99052632-99052651 None:intergenic 50.0%
GGTCATCAGATTGTGCCCTA+TGG - chr3.4:99053971-99053990 MS.gene66837:intron 50.0%
GTAGCCATAACCTCCAACGT+AGG + chr3.4:99053093-99053112 None:intergenic 50.0%
GTGAGCATAGCTCAGTTGGT+AGG - chr3.4:99054365-99054384 MS.gene66837:intron 50.0%
TAGGGCACAATCTGATGACC+TGG + chr3.4:99053971-99053990 None:intergenic 50.0%
TATGATCAACACGGTGGCAC+CGG + chr3.4:99052653-99052672 None:intergenic 50.0%
TATTCACATGGGAGAGGGGT+GGG + chr3.4:99054324-99054343 None:intergenic 50.0%
TCCTGTGAGCATAGCTCAGT+TGG - chr3.4:99054361-99054380 MS.gene66837:CDS 50.0%
TTATTCACATGGGAGAGGGG+TGG + chr3.4:99054325-99054344 None:intergenic 50.0%
TTGATCCTGAACTTGGACCC+TGG + chr3.4:99052218-99052237 None:intergenic 50.0%
! GAACGTGGGTTTTCCATTCC+TGG - chr3.4:99054286-99054305 MS.gene66837:CDS 50.0%
!! AATTGTGGTTGGCCTGGAAC+TGG + chr3.4:99055138-99055157 None:intergenic 50.0%
!! CTTCGTTGGTGGATTGGCAT+GGG - chr3.4:99052299-99052318 MS.gene66837:intron 50.0%
!! TCTTCGTTGGTGGATTGGCA+TGG - chr3.4:99052298-99052317 MS.gene66837:intron 50.0%
AACCTCCAACGTAGGCACCA+CGG + chr3.4:99053085-99053104 None:intergenic 55.0%
ACCGGACGATCCAAAGGCTA+CGG - chr3.4:99052402-99052421 MS.gene66837:intron 55.0%
ACCGTAGCCTTTGGATCGTC+CGG + chr3.4:99052406-99052425 None:intergenic 55.0%
AGCGGTCCTGACCAAACAGA+TGG - chr3.4:99055186-99055205 MS.gene66837:CDS 55.0%
CAGCATCCCACTCACCCATA+AGG + chr3.4:99053161-99053180 None:intergenic 55.0%
CATCCCACTCACCCATAAGG+AGG + chr3.4:99053158-99053177 None:intergenic 55.0%
CGTGGACCCCCAATCTCATA+TGG - chr3.4:99052855-99052874 MS.gene66837:intron 55.0%
GCATACCAGGGTCCAAGTTC+AGG - chr3.4:99052210-99052229 MS.gene66837:CDS 55.0%
GCTGTATAACCATGACCGCC+AGG + chr3.4:99053929-99053948 None:intergenic 55.0%
GGAATTGCTGCACCAGTTCC+AGG - chr3.4:99055123-99055142 MS.gene66837:CDS 55.0%
GGTGACTTTCCGTGATCCAG+AGG - chr3.4:99052749-99052768 MS.gene66837:intron 55.0%
GGTGCCTACGTTGGAGGTTA+TGG - chr3.4:99053086-99053105 MS.gene66837:intron 55.0%
TATGAGATTGGGGGTCCACG+TGG + chr3.4:99052855-99052874 None:intergenic 55.0%
TCAGAACGTGGGGAACACAC+TGG - chr3.4:99054245-99054264 MS.gene66837:intron 55.0%
TCCTAGCAGCCTCTGGATCA+CGG + chr3.4:99052761-99052780 None:intergenic 55.0%
TGGGACTTGAATGGCAGGCA+CGG + chr3.4:99054029-99054048 None:intergenic 55.0%
TTGGGTCAAGCTGTTCCTGG+CGG - chr3.4:99053911-99053930 MS.gene66837:intron 55.0%
! GTGAATAAGTGGGGTGTCCG+AGG + chr3.4:99054427-99054446 None:intergenic 55.0%
! TGTGACTCGGGCCATTCCTT+TGG - chr3.4:99054092-99054111 MS.gene66837:intron 55.0%
ACCTCCAACGTAGGCACCAC+GGG + chr3.4:99053084-99053103 None:intergenic 60.0%
CAAGCTGTTCCTGGCGGTCA+TGG - chr3.4:99053917-99053936 MS.gene66837:intron 60.0%
GGAAGTTTGCAACCAGCCCG+TGG - chr3.4:99053065-99053084 MS.gene66837:intron 60.0%
GGAATGGCCCGAGTCACAAG+TGG + chr3.4:99054090-99054109 None:intergenic 60.0%
GGACAGAGGACTGTACCGCT+AGG + chr3.4:99054055-99054074 None:intergenic 60.0%
GGGACTTGAATGGCAGGCAC+GGG + chr3.4:99054028-99054047 None:intergenic 60.0%
GGTGTCCGAGGTTCGAAACC+CGG + chr3.4:99054415-99054434 None:intergenic 60.0%
TCCGTGATCCAGAGGCTGCT+AGG - chr3.4:99052757-99052776 MS.gene66837:intron 60.0%
AGGGGCCGGGTTTCGAACCT+CGG - chr3.4:99054407-99054426 MS.gene66837:intron 65.0%
CAGGCTCTCCTAGCAGCCTC+TGG + chr3.4:99052768-99052787 None:intergenic 65.0%
GCTTCGCATGGTCGTCCACG+TGG - chr3.4:99052837-99052856 MS.gene66837:intron 65.0%
CCAACGTAGGCACCACGGGC+TGG + chr3.4:99053080-99053099 None:intergenic 70.0%
CCAGCCCGTGGTGCCTACGT+TGG - chr3.4:99053077-99053096 MS.gene66837:intron 70.0%
GCCCGTGGTGCCTACGTTGG+AGG - chr3.4:99053080-99053099 MS.gene66837:intron 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 99052207 99055212 99052207 ID=MS.gene66837
chr3.4 mRNA 99052207 99055212 99052207 ID=MS.gene66837.t1;Parent=MS.gene66837
chr3.4 exon 99054991 99055212 99054991 ID=MS.gene66837.t1.exon1;Parent=MS.gene66837.t1
chr3.4 CDS 99054991 99055212 99054991 ID=cds.MS.gene66837.t1;Parent=MS.gene66837.t1
chr3.4 exon 99054543 99054669 99054543 ID=MS.gene66837.t1.exon2;Parent=MS.gene66837.t1
chr3.4 CDS 99054543 99054669 99054543 ID=cds.MS.gene66837.t1;Parent=MS.gene66837.t1
chr3.4 exon 99054254 99054383 99054254 ID=MS.gene66837.t1.exon3;Parent=MS.gene66837.t1
chr3.4 CDS 99054254 99054383 99054254 ID=cds.MS.gene66837.t1;Parent=MS.gene66837.t1
chr3.4 exon 99053692 99053728 99053692 ID=MS.gene66837.t1.exon4;Parent=MS.gene66837.t1
chr3.4 CDS 99053692 99053728 99053692 ID=cds.MS.gene66837.t1;Parent=MS.gene66837.t1
chr3.4 exon 99053361 99053601 99053361 ID=MS.gene66837.t1.exon5;Parent=MS.gene66837.t1
chr3.4 CDS 99053361 99053601 99053361 ID=cds.MS.gene66837.t1;Parent=MS.gene66837.t1
chr3.4 exon 99052207 99052295 99052207 ID=MS.gene66837.t1.exon6;Parent=MS.gene66837.t1
chr3.4 CDS 99052207 99052295 99052207 ID=cds.MS.gene66837.t1;Parent=MS.gene66837.t1
Gene Sequence

>MS.gene66837

ATGGCATACCAGGGTCCAAGTTCAGGATCAAGTTCGAGTTCTGGGTTTCAATTGTTGAATTCTCCTTTTGGAGATACTACTTATACTAAAGTCTTCGTTGGTGGATTGGCATGGGAGACTCAGAGTGAAACTATGCGCCGTTACTTTGAACAATTTGGAGATATTCTTGAAGCCGTCGTCATCACTGATAAGAACACCGGACGATCCAAAGGCTACGGTTTTGTGAGCATCTTTGCTTTTATCAATTCATTTATTAGTTGTTGACATTACTATTACTACCCTATTTCTTTCTTTGTTTATATTCTATCATATAAGGCTATAACTCATTTTATTACCAAAACATTGTTGAACATTGTAATTTGAGAAAATGATGACATGGAATCACATATCTATGTGTCGGACCCTATTGGACACTTGACACACCTCTGATCAGATCAGAAGTGCCGGTGCCACCGTGTTGATCATACTAGCATGGTTGCATTTTTTTATTTACTATAGAATGACTTGGTTTCTATCTTATATGCATGGAATTGGAGGGTATAGGTGACTTTCCGTGATCCAGAGGCTGCTAGGAGAGCCTGTGCTGATCCAAGTCCTGTTATTGATGGTAGAAGGGCTAATTGTAACTTGGCTTCGCATGGTCGTCCACGTGGACCCCCAATCTCATATGGTATGCTATTATTTCTTAACAAATGAGTTTATTCTCAAGTGATGTTATATTATTCTTTGATATGCATAGCAGTCTGCTGCAACTTGAAGTTTCCAGAATACTCTTCCTACATAGAATATACATTGCTTCTATTTTCTGATTTCTTCTTATCTTTTATAGGACGGGTAAGACCAGCATCTCCTTATGTTGGAAGTTTGCAACCAGCCCGTGGTGCCTACGTTGGAGGTTATGGCTACCAACAACCACTTTCCTATGGCTATCAACAGGGAATGGTTTATCCTCCTTATGGGTGAGTGGGATGCTGTTGCACACTATTGCAAAAACTGGATTTGAAGATTATTTTCACCGTCAATCTGCTAATAATAATAATGCTCTTGCCTTTCCTGTGTTATGGTTCTTTGTGTCCATGCTTATTTTAGCAGTTACATAACTCTTTTTGAACCCAGTTATCGTGTTTTTTTGACTGGTATATGTCAGTGATGAGGTCAACTAGACCTATACAACATATTCTTGCAAGTACTTGATTAGTAGTTAGTTAGGAGATGAAAATCCCGAGATTTGGTAATCACTGGTCCACTACAACAACAACAACAACCAAGCCTTATCTCACTAAGTGGGGCGTAATCACTGGTCCACTGTATATGGAAAACCTATGTCAATTGGATGTAATAAACTAATGACCATTTAATATTAGTCTCCATGTGGGTAAGGTGTTCCTAACCACATCTGCATTGTGCATCTAAATGTATCACGCCAGAATCATGCTTTAGAGGTGATCTTTTCAACATTATATTTGGTTGTTTTATCTTTTCAGGTATACGGCATATGGACCTGAATACGTATACCCACAGGTCATATCAGTTTTTTTTTTTATTTGGCTTAGGATAAATGTCTATTATTTGTGAAATGCTGACCTTTTCAACTTCTACCGTGTCCAATAGGGTATGTACAACCCTTATGCTGGTCAACAACAGTACCTTCAGATATATGGTGTGCCTGGTGCGATGAATACAACTATTTATCCTTATGGACAATTGGGTCAAGCTGTTCCTGGCGGTCATGGTTATACAGCAATGCAGGGTTATACAGTACCAGGTCATCAGATTGTGCCCTATGGTGGGTCCAATGTTAATGCAATAACAACTTCACCCGTGCCTGCCATTCAAGTCCCATATCCTAGCGGTACAGTCCTCTGTCCAAGAAAGAATTTCCACTTGTGACTCGGGCCATTCCTTTGGAATAACTTTCCCAGGAAAACAATAATGATAATTTTATTCCTAGGATTTTTTCAAAGAAATGAACCATTACATTGATAGACCTTAAGCCTCATTACTCACAAAAAAGGAAACCGTTACATTAAATAATTTATCAGAACGTGGGGAACACACTGGTAAAATGAGTTTTGCTCTGAACGTGGGTTTTCCATTCCTGGTAATAGAATTTTCCCACCCCTCTCCCATGTGAATAAAAATGACTAGAACATGTCCTGTGAGCATAGCTCAGTTGGTAGGACAATGCATTATTATATGCAGGGGCCGGGTTTCGAACCTCGGACACCCCACTTATTCACCTTATAAGGTGAATTCTAGCCACTAGGCTACCTGACCAAAAAAAAAAAATTTACTAGAACATGGGTTAAGTAGATTCCTCGGTATTATTTATTCCTAGGCATATTATTTTTGTTCCTTCCTACCAAACACAGGAATTCCTTTTTCCCATCCTCTATTCCCGGGAATTTATATTTTATTTTTTTATTTCTGAAACAAATGCTCCCTTTTTGTTTATGAACAGCCATCTTTGGTGGATATTCTTTGAGAAACAAAAGCCCATATATATTGGAATTAACATTCATTTGAAAACATATTAGCAAATAACCTTCAATTCTTAACAAGCATTGATTGGCCAGATCACATACACTTATCAGCTGTTGGTATATAAATAAATGAGATTAATGAATGATACGACATATGTAGGCAATTTCAGTTGTCAAACCCTAAATTTATGTACAAAATTAAGGGTTTTGTAATATATGATAGAAGGTTGGGTTTATTTAATTAAAAAATATAGGTAATTGAAGTTCTGATCAACAGGAAAGTAGCTAGTCTGTAAAGAATGGAGATTCGTTTTATATGTACAGTGTTGTAAGATTGCATATAGAGCAAGTCATTAAAATCTATTGATTATTATTTAGCACAAAACTTTAAATTCTGTGCAAATAGGTGTGATTCGTAAGTATTTGATTTTCAGGAATTGCTGCACCAGTTCCAGGCCAACCACAATTTATTGTTTCTCCTCAGTTTATGCAAGGTAGCGGTCCTGACCAAACAGATGGTTGA
Protein sequence

>MS.gene66837.t1

MAYQGPSSGSSSSSGFQLLNSPFGDTTYTKVFVGGLAWETQSETMRRYFEQFGDILEAVVITDKNTGRSKGYGFVTFRDPEAARRACADPSPVIDGRRANCNLASHGRPRGPPISYGRVRPASPYVGSLQPARGAYVGGYGYQQPLSYGYQQGMVYPPYGYTAYGPEYVYPQGMYNPYAGQQQYLQIYGVPGAMNTTIYPYGQLGQAVPGGHGYTAMQGYTVPGHQIVPYGGSNVNAITTSPVPAIQVPYPSGIAAPVPGQPQFIVSPQFMQGSGPDQTDG