Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66844.t1 | XP_013465955.1 | 100 | 107 | 0 | 0 | 1 | 107 | 46 | 152 | 1.50E-49 | 205.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66844.t1 | P08927 | 98.1 | 107 | 2 | 0 | 1 | 107 | 49 | 155 | 3.0e-51 | 202.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66844.t1 | A0A072VEG8 | 100.0 | 107 | 0 | 0 | 1 | 107 | 46 | 152 | 1.1e-49 | 205.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050301 | MS.gene66844 | 0.801045 | 1.08E-48 | -1.69E-46 |
| MS.gene051663 | MS.gene66844 | 0.80576 | 1.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66844.t1 | MTR_0991s0010 | 98.131 | 107 | 2 | 0 | 1 | 107 | 46 | 152 | 3.89e-71 | 210 |
| MS.gene66844.t1 | MTR_1g017380 | 100.000 | 107 | 0 | 0 | 1 | 107 | 46 | 152 | 4.39e-67 | 211 |
| MS.gene66844.t1 | MTR_1g017390 | 98.131 | 107 | 2 | 0 | 1 | 107 | 46 | 152 | 7.58e-66 | 208 |
| MS.gene66844.t1 | MTR_4g057970 | 56.731 | 104 | 42 | 2 | 2 | 105 | 49 | 149 | 2.94e-33 | 121 |
| MS.gene66844.t1 | MTR_1g090140 | 52.381 | 105 | 48 | 1 | 2 | 106 | 34 | 136 | 5.35e-30 | 112 |
| MS.gene66844.t1 | MTR_1g090130 | 50.476 | 105 | 50 | 1 | 2 | 106 | 34 | 136 | 1.01e-28 | 108 |
| MS.gene66844.t1 | MTR_4g084130 | 51.456 | 103 | 47 | 2 | 3 | 105 | 42 | 141 | 1.13e-28 | 108 |
| MS.gene66844.t1 | MTR_8g017080 | 45.283 | 106 | 52 | 2 | 6 | 106 | 33 | 137 | 2.37e-23 | 93.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66844.t1 | AT5G56500 | 94.340 | 106 | 6 | 0 | 2 | 107 | 52 | 157 | 2.13e-62 | 199 |
| MS.gene66844.t1 | AT5G56500 | 94.340 | 106 | 6 | 0 | 2 | 107 | 52 | 157 | 2.13e-62 | 199 |
| MS.gene66844.t1 | AT3G13470 | 93.396 | 106 | 7 | 0 | 2 | 107 | 52 | 157 | 4.37e-62 | 199 |
| MS.gene66844.t1 | AT1G55490 | 92.453 | 106 | 8 | 0 | 2 | 107 | 56 | 161 | 1.41e-61 | 197 |
| MS.gene66844.t1 | AT1G55490 | 92.453 | 106 | 8 | 0 | 2 | 107 | 56 | 161 | 1.41e-61 | 197 |
| MS.gene66844.t1 | AT1G55490 | 92.453 | 106 | 8 | 0 | 2 | 107 | 56 | 161 | 1.41e-61 | 197 |
| MS.gene66844.t1 | AT1G55490 | 92.453 | 106 | 8 | 0 | 2 | 107 | 56 | 161 | 1.41e-61 | 197 |
| MS.gene66844.t1 | AT1G55490 | 92.453 | 106 | 8 | 0 | 2 | 107 | 56 | 161 | 1.41e-61 | 197 |
| MS.gene66844.t1 | AT1G26230 | 71.698 | 106 | 30 | 0 | 2 | 107 | 40 | 145 | 2.34e-49 | 163 |
| MS.gene66844.t1 | AT1G26230 | 71.698 | 106 | 30 | 0 | 2 | 107 | 40 | 145 | 6.56e-49 | 163 |
| MS.gene66844.t1 | AT1G26230 | 71.698 | 106 | 30 | 0 | 2 | 107 | 40 | 145 | 1.56e-48 | 163 |
| MS.gene66844.t1 | AT1G26230 | 72.727 | 88 | 24 | 0 | 20 | 107 | 6 | 93 | 2.86e-39 | 137 |
| MS.gene66844.t1 | AT1G26230 | 75.000 | 84 | 21 | 0 | 24 | 107 | 2 | 85 | 1.07e-38 | 135 |
| MS.gene66844.t1 | AT2G28000 | 57.282 | 103 | 41 | 2 | 3 | 105 | 49 | 148 | 6.29e-33 | 120 |
| MS.gene66844.t1 | AT3G23990 | 52.381 | 105 | 48 | 1 | 2 | 106 | 33 | 135 | 1.28e-30 | 114 |
| MS.gene66844.t1 | AT2G33210 | 49.524 | 105 | 51 | 1 | 2 | 106 | 34 | 136 | 2.05e-29 | 110 |
| MS.gene66844.t1 | AT2G33210 | 49.524 | 105 | 51 | 1 | 2 | 106 | 34 | 136 | 2.40e-29 | 110 |
| MS.gene66844.t1 | AT5G18820 | 51.456 | 103 | 47 | 2 | 2 | 104 | 35 | 134 | 7.33e-28 | 105 |
| MS.gene66844.t1 | AT5G18820 | 51.456 | 103 | 47 | 2 | 2 | 104 | 35 | 134 | 1.71e-27 | 105 |
| MS.gene66844.t1 | AT3G13860 | 49.524 | 105 | 51 | 1 | 2 | 106 | 33 | 135 | 2.42e-27 | 104 |
Find 31 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTACTACCTCTGTTGTTT+TGG | 0.233024 | 1.2:-5466261 | MS.gene66844:CDS |
| TGAGAATATTGGTGCTAAAT+TGG | 0.241648 | 1.2:-5466327 | MS.gene66844:CDS |
| AGAACAACATTCCTTCCTTT+AGG | 0.335817 | 1.2:+5466940 | None:intergenic |
| TGTGAACAAGCTTGCGGATT+TGG | 0.336342 | 1.2:-5466980 | MS.gene66844:CDS |
| ACACCATCATTAACAATCTT+AGG | 0.351092 | 1.2:+5466898 | None:intergenic |
| GTTGTTCTTGAGAGCAAGTA+TGG | 0.388399 | 1.2:-5466925 | MS.gene66844:CDS |
| AGCCGCCAAGACAAATGATT+TGG | 0.408630 | 1.2:-5466294 | MS.gene66844:CDS |
| TTAGCACCAATATTCTCAAC+TGG | 0.436766 | 1.2:+5466332 | None:intergenic |
| GAGGATCCAGTTGAGAATAT+TGG | 0.438153 | 1.2:-5466338 | MS.gene66844:CDS |
| TGGTGCTAAATTGGTTAGAC+AGG | 0.448444 | 1.2:-5466318 | MS.gene66844:CDS |
| GGTGTTACTCTTGGTCCTAA+AGG | 0.456739 | 1.2:-5466955 | MS.gene66844:CDS |
| GCCTGCCAAATCATTTGTCT+TGG | 0.460705 | 1.2:+5466289 | None:intergenic |
| TCTTATTGCAGAAGGTGTTA+AGG | 0.461521 | 1.2:-5466231 | MS.gene66844:CDS |
| TTTGTTGAAATACAACTCCT+TGG | 0.469848 | 1.2:+5467122 | None:intergenic |
| GAGTTGTATTTCAACAAAGA+TGG | 0.475978 | 1.2:-5467117 | MS.gene66844:CDS |
| GATTTGGTTGGTGTTACTCT+TGG | 0.477460 | 1.2:-5466964 | MS.gene66844:CDS |
| TAAACAAACACACACCTCTC+TGG | 0.488579 | 1.2:+5466867 | None:intergenic |
| TGCCAAATCATTTGTCTTGG+CGG | 0.501395 | 1.2:+5466292 | None:intergenic |
| GCTCAGGGTCTTATTGCAGA+AGG | 0.514992 | 1.2:-5466239 | MS.gene66844:CDS |
| ACAAATGATTTGGCAGGCGA+TGG | 0.521838 | 1.2:-5466284 | MS.gene66844:CDS |
| TCTCCTAAGATTGTTAATGA+TGG | 0.522934 | 1.2:-5466901 | MS.gene66844:CDS |
| GCCAAGACAAATGATTTGGC+AGG | 0.531683 | 1.2:-5466290 | MS.gene66844:CDS |
| TTACTCTTGGTCCTAAAGGA+AGG | 0.571092 | 1.2:-5466951 | MS.gene66844:CDS |
| AACAAGCTTGCGGATTTGGT+TGG | 0.576745 | 1.2:-5466976 | MS.gene66844:CDS |
| TTCCGCTATCAAGAAACTCC+AGG | 0.579355 | 1.2:-5467094 | MS.gene66844:intron |
| TAAAGTCTCAGCCATGGCCA+AGG | 0.594241 | 1.2:-5467139 | None:intergenic |
| CACCTGGAGTTTCTTGATAG+CGG | 0.605493 | 1.2:+5467092 | None:intergenic |
| GAAATACAACTCCTTGGCCA+TGG | 0.609599 | 1.2:+5467128 | None:intergenic |
| GAATGGTGTGAACAAGCTTG+CGG | 0.665076 | 1.2:-5466986 | MS.gene66844:CDS |
| TGGTGTCACTGTTGCCAGAG+AGG | 0.672652 | 1.2:-5466881 | MS.gene66844:intron |
| ACCCTGAGCCAAAACAACAG+AGG | 0.733850 | 1.2:+5466253 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTACTTGAATTATTGTTTT+AGG | - | chr1.2:5466762-5466781 | MS.gene66844:intron | 15.0% |
| !!! | TTTACTTGAATTATTGTTTT+TGG | - | chr1.2:5466762-5466781 | MS.gene66844:intron | 15.0% |
| !!! | TTTTAGTGAATTAATAATGT+GGG | - | chr1.2:5466933-5466952 | MS.gene66844:CDS | 15.0% |
| !!! | TTTTAGTGAATTATTTATGT+GGG | - | chr1.2:5466661-5466680 | MS.gene66844:intron | 15.0% |
| !!! | TTTTTAGTGAATTAATAATG+TGG | - | chr1.2:5466932-5466951 | MS.gene66844:CDS | 15.0% |
| !!! | TTTTTAGTGAATTATTTATG+TGG | - | chr1.2:5466660-5466679 | MS.gene66844:intron | 15.0% |
| !! | TTTAGTGAATTAATAATGTG+GGG | - | chr1.2:5466934-5466953 | MS.gene66844:CDS | 20.0% |
| !! | TTTAGTGAATTATTTATGTG+GGG | - | chr1.2:5466662-5466681 | MS.gene66844:intron | 20.0% |
| !!! | ATTTATTTTGTCTGAGTTTA+GGG | - | chr1.2:5466731-5466750 | MS.gene66844:intron | 20.0% |
| !!! | TATTTATTTTGTCTGAGTTT+AGG | - | chr1.2:5466730-5466749 | MS.gene66844:intron | 20.0% |
| !!! | TTCACTTGAATTAATGTTTT+TGG | - | chr1.2:5466584-5466603 | MS.gene66844:intron | 20.0% |
| !!! | TTCACTTGAATTATTGTTTT+TGG | - | chr1.2:5466702-5466721 | MS.gene66844:intron | 20.0% |
| ! | TGTTTACATTTGCGTTAAAA+AGG | - | chr1.2:5466974-5466993 | MS.gene66844:CDS | 25.0% |
| !! | TTTTGGTGAATTAGTTATGT+GGG | - | chr1.2:5466779-5466798 | MS.gene66844:intron | 25.0% |
| !!! | GTTTAATTTGTCTGAGTTTA+GGG | - | chr1.2:5466613-5466632 | MS.gene66844:intron | 25.0% |
| !!! | TGTTTAATTTGTCTGAGTTT+AGG | - | chr1.2:5466612-5466631 | MS.gene66844:intron | 25.0% |
| !!! | TTTGTGTTTTTGTTACAGAA+TGG | - | chr1.2:5466340-5466359 | MS.gene66844:CDS | 25.0% |
| !!! | TTTTAGGTGAATTAGTTATG+TGG | - | chr1.2:5466855-5466874 | MS.gene66844:intron | 25.0% |
| !!! | TTTTTGGTGAATTAGTTATG+TGG | - | chr1.2:5466778-5466797 | MS.gene66844:intron | 25.0% |
| ACACCATCATTAACAATCTT+AGG | + | chr1.2:5466448-5466467 | None:intergenic | 30.0% | |
| ACATCACAGAATTCAAAAAC+AGG | + | chr1.2:5466508-5466527 | None:intergenic | 30.0% | |
| GAGTTGTATTTCAACAAAGA+TGG | - | chr1.2:5466226-5466245 | MS.gene66844:CDS | 30.0% | |
| TCTCCTAAGATTGTTAATGA+TGG | - | chr1.2:5466442-5466461 | MS.gene66844:intron | 30.0% | |
| TTACATTTGCGTTAAAAAGG+TGG | - | chr1.2:5466977-5466996 | MS.gene66844:CDS | 30.0% | |
| TTTGGTGAATTAGTTATGTG+GGG | - | chr1.2:5466780-5466799 | MS.gene66844:intron | 30.0% | |
| TTTGTTGAAATACAACTCCT+TGG | + | chr1.2:5466224-5466243 | None:intergenic | 30.0% | |
| ! | ATTATTTATGTGGGGTGTTT+AGG | - | chr1.2:5466670-5466689 | MS.gene66844:intron | 30.0% |
| ! | TTATTTATGTGGGGTGTTTA+GGG | - | chr1.2:5466671-5466690 | MS.gene66844:intron | 30.0% |
| !! | TGAGAATATTGGTGCTAAAT+TGG | - | chr1.2:5467016-5467035 | MS.gene66844:intron | 30.0% |
| !!! | TTCGTTTTTTGAGCATGATT+GGG | + | chr1.2:5466284-5466303 | None:intergenic | 30.0% |
| !!! | TTTCGTTTTTTGAGCATGAT+TGG | + | chr1.2:5466285-5466304 | None:intergenic | 30.0% |
| AGAACAACATTCCTTCCTTT+AGG | + | chr1.2:5466406-5466425 | None:intergenic | 35.0% | |
| TTAGCACCAATATTCTCAAC+TGG | + | chr1.2:5467014-5467033 | None:intergenic | 35.0% | |
| !! | AACTACTACCTCTGTTGTTT+TGG | - | chr1.2:5467082-5467101 | MS.gene66844:intron | 35.0% |
| !! | GTTTTGATGTTGGGTGTTTA+GGG | - | chr1.2:5466885-5466904 | MS.gene66844:CDS | 35.0% |
| !! | TCTTATTGCAGAAGGTGTTA+AGG | - | chr1.2:5467112-5467131 | MS.gene66844:CDS | 35.0% |
| !!! | GGAGTGTTTGTTTTGATGTT+GGG | - | chr1.2:5466876-5466895 | MS.gene66844:intron | 35.0% |
| !!! | TGGAGTGTTTGTTTTGATGT+TGG | - | chr1.2:5466875-5466894 | MS.gene66844:intron | 35.0% |
| !!! | TGTTTTGATGTTGGGTGTTT+AGG | - | chr1.2:5466884-5466903 | MS.gene66844:CDS | 35.0% |
| GAGGATCCAGTTGAGAATAT+TGG | - | chr1.2:5467005-5467024 | MS.gene66844:intron | 40.0% | |
| GTTGTTCTTGAGAGCAAGTA+TGG | - | chr1.2:5466418-5466437 | MS.gene66844:intron | 40.0% | |
| TAAACAAACACACACCTCTC+TGG | + | chr1.2:5466479-5466498 | None:intergenic | 40.0% | |
| TGCCAAATCATTTGTCTTGG+CGG | + | chr1.2:5467054-5467073 | None:intergenic | 40.0% | |
| TTACTCTTGGTCCTAAAGGA+AGG | - | chr1.2:5466392-5466411 | MS.gene66844:intron | 40.0% | |
| TTGCGTTAAAAAGGTGGAGT+TGG | - | chr1.2:5466983-5467002 | MS.gene66844:CDS | 40.0% | |
| ! | TGGTGCTAAATTGGTTAGAC+AGG | - | chr1.2:5467025-5467044 | MS.gene66844:intron | 40.0% |
| ! | TTTGTCTGAGTTTAGGGTCA+AGG | - | chr1.2:5466619-5466638 | MS.gene66844:intron | 40.0% |
| !! | GATTTGGTTGGTGTTACTCT+TGG | - | chr1.2:5466379-5466398 | MS.gene66844:intron | 40.0% |
| AGCCGCCAAGACAAATGATT+TGG | - | chr1.2:5467049-5467068 | MS.gene66844:intron | 45.0% | |
| CACCTGGAGTTTCTTGATAG+CGG | + | chr1.2:5466254-5466273 | None:intergenic | 45.0% | |
| CGTTAAAAAGGTGGAGTTGG+AGG | - | chr1.2:5466986-5467005 | MS.gene66844:CDS | 45.0% | |
| GAATGGTGTGAACAAGCTTG+CGG | - | chr1.2:5466357-5466376 | MS.gene66844:intron | 45.0% | |
| GCCTGCCAAATCATTTGTCT+TGG | + | chr1.2:5467057-5467076 | None:intergenic | 45.0% | |
| GCTATCAAGAAACTCCAGGT+GGG | - | chr1.2:5466253-5466272 | MS.gene66844:CDS | 45.0% | |
| TGTGAACAAGCTTGCGGATT+TGG | - | chr1.2:5466363-5466382 | MS.gene66844:intron | 45.0% | |
| TTCCGCTATCAAGAAACTCC+AGG | - | chr1.2:5466249-5466268 | MS.gene66844:CDS | 45.0% | |
| ! | AACAAGCTTGCGGATTTGGT+TGG | - | chr1.2:5466367-5466386 | MS.gene66844:intron | 45.0% |
| ! | ACAAATGATTTGGCAGGCGA+TGG | - | chr1.2:5467059-5467078 | MS.gene66844:intron | 45.0% |
| ! | GCCAAGACAAATGATTTGGC+AGG | - | chr1.2:5467053-5467072 | MS.gene66844:intron | 45.0% |
| !! | GGTGTTACTCTTGGTCCTAA+AGG | - | chr1.2:5466388-5466407 | MS.gene66844:intron | 45.0% |
| !!! | ACCTCTGTTGTTTTGGCTCA+GGG | - | chr1.2:5467089-5467108 | MS.gene66844:intron | 45.0% |
| !!! | TACCTCTGTTGTTTTGGCTC+AGG | - | chr1.2:5467088-5467107 | MS.gene66844:intron | 45.0% |
| ACCCTGAGCCAAAACAACAG+AGG | + | chr1.2:5467093-5467112 | None:intergenic | 50.0% | |
| ATGATTGGGAAGAACCCACC+TGG | + | chr1.2:5466270-5466289 | None:intergenic | 50.0% | |
| CGCTATCAAGAAACTCCAGG+TGG | - | chr1.2:5466252-5466271 | MS.gene66844:CDS | 50.0% | |
| ! | GTGGGGTGTTTGCTTTGATG+CGG | - | chr1.2:5466797-5466816 | MS.gene66844:intron | 50.0% |
| !! | GCTCAGGGTCTTATTGCAGA+AGG | - | chr1.2:5467104-5467123 | MS.gene66844:CDS | 50.0% |
| !! | TGGTGTCACTGTTGCCAGAG+AGG | - | chr1.2:5466462-5466481 | MS.gene66844:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 5466217 | 5467148 | 5466217 | ID=MS.gene66844 |
| chr1.2 | mRNA | 5466217 | 5467148 | 5466217 | ID=MS.gene66844.t1;Parent=MS.gene66844 |
| chr1.2 | exon | 5467095 | 5467148 | 5467095 | ID=MS.gene66844.t1.exon1;Parent=MS.gene66844.t1 |
| chr1.2 | CDS | 5467095 | 5467148 | 5467095 | ID=cds.MS.gene66844.t1;Parent=MS.gene66844.t1 |
| chr1.2 | exon | 5466882 | 5467007 | 5466882 | ID=MS.gene66844.t1.exon2;Parent=MS.gene66844.t1 |
| chr1.2 | CDS | 5466882 | 5467007 | 5466882 | ID=cds.MS.gene66844.t1;Parent=MS.gene66844.t1 |
| chr1.2 | exon | 5466217 | 5466369 | 5466217 | ID=MS.gene66844.t1.exon3;Parent=MS.gene66844.t1 |
| chr1.2 | CDS | 5466217 | 5466369 | 5466217 | ID=cds.MS.gene66844.t1;Parent=MS.gene66844.t1 |
| Gene Sequence |
| Protein sequence |
