Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66866.t1 | XP_003622734.2 | 98.9 | 92 | 1 | 0 | 1 | 92 | 465 | 556 | 3.30E-47 | 197.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66866.t1 | Q9SU40 | 68.8 | 93 | 28 | 1 | 1 | 92 | 463 | 555 | 2.8e-34 | 145.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66866.t1 | G7KX02 | 98.9 | 92 | 1 | 0 | 1 | 92 | 465 | 556 | 2.4e-47 | 197.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051248 | MS.gene66866 | 0.802939 | 4.42E-49 | -1.69E-46 |
| MS.gene059154 | MS.gene66866 | 0.823824 | 1.12E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTCTCTTTAGGTCTTTCC+TGG | 0.176907 | 7.4:+50627263 | MS.gene66866:intron |
| TCTGATTGTAGCATGGATTT+TGG | 0.177553 | 7.4:+50626289 | None:intergenic |
| CAACTTGAAGATAAGATTCT+TGG | 0.239119 | 7.4:-50627370 | None:intergenic |
| TTTAGGTCTTTCCTGGGGCT+TGG | 0.295657 | 7.4:+50627270 | MS.gene66866:intron |
| CCACAGTATATTGCATTATC+AGG | 0.312549 | 7.4:-50627428 | None:intergenic |
| CTCTTGACAATGCTGGCATT+TGG | 0.381859 | 7.4:+50627309 | MS.gene66866:CDS |
| TAAGGAACTCTTACCTTTGA+AGG | 0.397795 | 7.4:-50627463 | None:intergenic |
| TGTCTCTTTAGGTCTTTCCT+GGG | 0.417536 | 7.4:+50627264 | MS.gene66866:intron |
| GCAGAAGTACTTACAATAAG+TGG | 0.423526 | 7.4:+50626329 | MS.gene66866:CDS |
| AACCTCAACTCCTGGTATCT+AGG | 0.437891 | 7.4:+50627347 | MS.gene66866:CDS |
| ATTGTCAAGAGATACTAAAA+TGG | 0.438940 | 7.4:-50627297 | None:intergenic |
| TTAGTATCTCTTGACAATGC+TGG | 0.464781 | 7.4:+50627302 | MS.gene66866:CDS |
| ATACCAGGAGTTGAGGTTCT+CGG | 0.471199 | 7.4:-50627342 | None:intergenic |
| ACCTTTGAAGGGAGGAAAGT+AGG | 0.478906 | 7.4:-50627451 | None:intergenic |
| GCCTACTTTCCTCCCTTCAA+AGG | 0.512657 | 7.4:+50627450 | MS.gene66866:CDS |
| AAGGAACTCTTACCTTTGAA+GGG | 0.533882 | 7.4:-50627462 | None:intergenic |
| GTGCCGAGAACCTCAACTCC+TGG | 0.548498 | 7.4:+50627339 | MS.gene66866:CDS |
| AGATTCTTGGCCTAGATACC+AGG | 0.550935 | 7.4:-50627357 | None:intergenic |
| GGCCTAGATACCAGGAGTTG+AGG | 0.554344 | 7.4:-50627349 | None:intergenic |
| AAAATGGCTGTCCAAGCCCC+AGG | 0.567280 | 7.4:-50627281 | None:intergenic |
| GTCTCTTTAGGTCTTTCCTG+GGG | 0.581453 | 7.4:+50627265 | MS.gene66866:intron |
| CAGAAGTACTTACAATAAGT+GGG | 0.604253 | 7.4:+50626330 | MS.gene66866:CDS |
| TGTGGCTCGCTGCACGACAC+AGG | 0.611173 | 7.4:+50626357 | MS.gene66866:CDS |
| GAACTCTTACCTTTGAAGGG+AGG | 0.622552 | 7.4:-50627459 | None:intergenic |
| TTACAATAAGTGGGACGGTG+TGG | 0.639857 | 7.4:+50626339 | MS.gene66866:CDS |
| AGTTGAGGTTCTCGGCACGA+AGG | 0.671541 | 7.4:-50627334 | None:intergenic |
| AGTACTTACAATAAGTGGGA+CGG | 0.675300 | 7.4:+50626334 | MS.gene66866:CDS |
| CCTGATAATGCAATATACTG+TGG | 0.696580 | 7.4:+50627428 | MS.gene66866:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAGAAAAAGTAACAAT+TGG | - | chr7.4:50627088-50627107 | None:intergenic | 15.0% |
| !! | ATTTCCAAATATAAAAAGAA+AGG | + | chr7.4:50626805-50626824 | MS.gene66866:intron | 15.0% |
| !! | CACATATATTAAAAAAAGTT+AGG | + | chr7.4:50626436-50626455 | MS.gene66866:intron | 15.0% |
| !! | GTTAGTTGTTTAAATATAAA+GGG | + | chr7.4:50627155-50627174 | MS.gene66866:intron | 15.0% |
| !! | TGTTAGTTGTTTAAATATAA+AGG | + | chr7.4:50627154-50627173 | MS.gene66866:intron | 15.0% |
| !! | TTTCCAAATATAAAAAGAAA+GGG | + | chr7.4:50626806-50626825 | MS.gene66866:intron | 15.0% |
| !!! | TTTCTTTTTATATTTGGAAA+TGG | - | chr7.4:50626806-50626825 | None:intergenic | 15.0% |
| !! | CTATAATTATAAGTCATCTT+TGG | - | chr7.4:50626749-50626768 | None:intergenic | 20.0% |
| !! | TTCCAAATATAAAAAGAAAG+GGG | + | chr7.4:50626807-50626826 | MS.gene66866:intron | 20.0% |
| !! | TTGGAAAGAAGAAAAAAAAA+AGG | - | chr7.4:50626730-50626749 | None:intergenic | 20.0% |
| ! | AGAAGTAAGTTGTAATGAAA+TGG | - | chr7.4:50627238-50627257 | None:intergenic | 25.0% |
| ! | ATATATGTGAAAACTGTCAA+AGG | - | chr7.4:50626425-50626444 | None:intergenic | 25.0% |
| ! | ATTGTCAAGAGATACTAAAA+TGG | - | chr7.4:50627300-50627319 | None:intergenic | 25.0% |
| !! | AAAGTTAGGAGTGTAATTAA+TGG | + | chr7.4:50626450-50626469 | MS.gene66866:intron | 25.0% |
| !! | CAAAGAAAACCTAAGTTTTA+AGG | - | chr7.4:50626908-50626927 | None:intergenic | 25.0% |
| !! | TACCCCTTTCTTTTTATATT+TGG | - | chr7.4:50626812-50626831 | None:intergenic | 25.0% |
| AAAATACCGAGCTTCAAATT+TGG | + | chr7.4:50626583-50626602 | MS.gene66866:intron | 30.0% | |
| CAACTTGAAGATAAGATTCT+TGG | - | chr7.4:50627373-50627392 | None:intergenic | 30.0% | |
| GAATAGAGTTGTTTGTTTCA+TGG | + | chr7.4:50626847-50626866 | MS.gene66866:intron | 30.0% | |
| GCGATTATAAATAGGTACAA+AGG | - | chr7.4:50626403-50626422 | None:intergenic | 30.0% | |
| TGCATGGGATGTATTAATAA+GGG | + | chr7.4:50626645-50626664 | MS.gene66866:intron | 30.0% | |
| TGCTGTTATTGCAAGTAAAT+TGG | + | chr7.4:50627180-50627199 | MS.gene66866:intron | 30.0% | |
| TGTCAAAGGCGATTATAAAT+AGG | - | chr7.4:50626411-50626430 | None:intergenic | 30.0% | |
| TTATCAAATAGCAGCTGTAA+TGG | + | chr7.4:50626951-50626970 | MS.gene66866:intron | 30.0% | |
| ! | GTATTAATAAGGGGTATGTT+TGG | + | chr7.4:50626655-50626674 | MS.gene66866:intron | 30.0% |
| !! | AGGAGTGTAATTAATGGATT+TGG | + | chr7.4:50626456-50626475 | MS.gene66866:intron | 30.0% |
| !! | CAGAAGTACTTACAATAAGT+GGG | + | chr7.4:50626330-50626349 | MS.gene66866:CDS | 30.0% |
| !! | TAATTTGCTTCCCATGAATT+TGG | + | chr7.4:50627102-50627121 | MS.gene66866:intron | 30.0% |
| AGTACTTACAATAAGTGGGA+CGG | + | chr7.4:50626334-50626353 | MS.gene66866:CDS | 35.0% | |
| CCACAGTATATTGCATTATC+AGG | - | chr7.4:50627431-50627450 | None:intergenic | 35.0% | |
| CTCAAAAGAAGGCTTCATTT+GGG | + | chr7.4:50626874-50626893 | MS.gene66866:intron | 35.0% | |
| CTGCATGGGATGTATTAATA+AGG | + | chr7.4:50626644-50626663 | MS.gene66866:intron | 35.0% | |
| CTTCTTTAACCTGTCTCTTT+AGG | + | chr7.4:50627253-50627272 | MS.gene66866:intron | 35.0% | |
| GCATGGGATGTATTAATAAG+GGG | + | chr7.4:50626646-50626665 | MS.gene66866:intron | 35.0% | |
| GCTTTGCTACCTTAAAACTT+AGG | + | chr7.4:50626896-50626915 | MS.gene66866:intron | 35.0% | |
| GTGTGTGATTCCAAATTCAT+GGG | - | chr7.4:50627115-50627134 | None:intergenic | 35.0% | |
| TGTGTGTGATTCCAAATTCA+TGG | - | chr7.4:50627116-50627135 | None:intergenic | 35.0% | |
| TTAGTATCTCTTGACAATGC+TGG | + | chr7.4:50627302-50627321 | MS.gene66866:CDS | 35.0% | |
| TTCATGGATTCCTCAAAAGA+AGG | + | chr7.4:50626863-50626882 | MS.gene66866:intron | 35.0% | |
| ! | AGCTGCTATTTGATAACACT+AGG | - | chr7.4:50626947-50626966 | None:intergenic | 35.0% |
| ! | CCTGATAATGCAATATACTG+TGG | + | chr7.4:50627428-50627447 | MS.gene66866:CDS | 35.0% |
| !! | AATGGTGCTATATAGCATAG+TGG | + | chr7.4:50626969-50626988 | MS.gene66866:intron | 35.0% |
| !! | GCAATTCTAAAGCGCTATAT+CGG | - | chr7.4:50627055-50627074 | None:intergenic | 35.0% |
| !! | GCAGAAGTACTTACAATAAG+TGG | + | chr7.4:50626329-50626348 | MS.gene66866:CDS | 35.0% |
| AAATGGTCCAGTCTCATGTT+GGG | - | chr7.4:50626789-50626808 | None:intergenic | 40.0% | |
| CCTCAAAAGAAGGCTTCATT+TGG | + | chr7.4:50626873-50626892 | MS.gene66866:intron | 40.0% | |
| GATGAACGTTGGATTGAGAA+CGG | - | chr7.4:50626614-50626633 | None:intergenic | 40.0% | |
| TGTCTCTTTAGGTCTTTCCT+GGG | + | chr7.4:50627264-50627283 | MS.gene66866:intron | 40.0% | |
| ! | CCAAATGAAGCCTTCTTTTG+AGG | - | chr7.4:50626876-50626895 | None:intergenic | 40.0% |
| !! | GTGCTGACTGCATAAATTTG+TGG | - | chr7.4:50626493-50626512 | None:intergenic | 40.0% |
| AACCTCAACTCCTGGTATCT+AGG | + | chr7.4:50627347-50627366 | MS.gene66866:CDS | 45.0% | |
| AACGTTGGATTGAGAACGGA+CGG | - | chr7.4:50626610-50626629 | None:intergenic | 45.0% | |
| AGATTCTTGGCCTAGATACC+AGG | - | chr7.4:50627360-50627379 | None:intergenic | 45.0% | |
| AGTGGCAGTTGGAATGAGAT+CGG | + | chr7.4:50626516-50626535 | MS.gene66866:intron | 45.0% | |
| AGTTGGAATGAGATCGGATG+GGG | + | chr7.4:50626522-50626541 | MS.gene66866:intron | 45.0% | |
| ATACCAGGAGTTGAGGTTCT+CGG | - | chr7.4:50627345-50627364 | None:intergenic | 45.0% | |
| CAGGAAAGACCTAAAGAGAC+AGG | - | chr7.4:50627265-50627284 | None:intergenic | 45.0% | |
| CAGTTGGAATGAGATCGGAT+GGG | + | chr7.4:50626521-50626540 | MS.gene66866:intron | 45.0% | |
| CTCTTGACAATGCTGGCATT+TGG | + | chr7.4:50627309-50627328 | MS.gene66866:CDS | 45.0% | |
| CTGTCTCTTTAGGTCTTTCC+TGG | + | chr7.4:50627263-50627282 | MS.gene66866:intron | 45.0% | |
| GAAATGGTCCAGTCTCATGT+TGG | - | chr7.4:50626790-50626809 | None:intergenic | 45.0% | |
| GTCTCTTTAGGTCTTTCCTG+GGG | + | chr7.4:50627265-50627284 | MS.gene66866:intron | 45.0% | |
| TTACAATAAGTGGGACGGTG+TGG | + | chr7.4:50626339-50626358 | MS.gene66866:CDS | 45.0% | |
| TTATGCAGTCAGCACATCAG+TGG | + | chr7.4:50626498-50626517 | MS.gene66866:intron | 45.0% | |
| ! | ACCTTTGAAGGGAGGAAAGT+AGG | - | chr7.4:50627454-50627473 | None:intergenic | 45.0% |
| ! | GACGGTCCAAATTTGAAGCT+CGG | - | chr7.4:50626592-50626611 | None:intergenic | 45.0% |
| GCAGTTGGAATGAGATCGGA+TGG | + | chr7.4:50626520-50626539 | MS.gene66866:intron | 50.0% | |
| GCCTACTTTCCTCCCTTCAA+AGG | + | chr7.4:50627450-50627469 | MS.gene66866:CDS | 50.0% | |
| GTCAGCACATCGATGAACGT+TGG | - | chr7.4:50626625-50626644 | None:intergenic | 50.0% | |
| GTCTTGACCCAACATGAGAC+TGG | + | chr7.4:50626779-50626798 | MS.gene66866:intron | 50.0% | |
| TTTAGGTCTTTCCTGGGGCT+TGG | + | chr7.4:50627270-50627289 | MS.gene66866:intron | 50.0% | |
| !! | CATCGATGTGCTGACTGCAT+GGG | + | chr7.4:50626630-50626649 | MS.gene66866:intron | 50.0% |
| !! | TCATCGATGTGCTGACTGCA+TGG | + | chr7.4:50626629-50626648 | MS.gene66866:intron | 50.0% |
| AAAATGGCTGTCCAAGCCCC+AGG | - | chr7.4:50627284-50627303 | None:intergenic | 55.0% | |
| AGTTGAGGTTCTCGGCACGA+AGG | - | chr7.4:50627337-50627356 | None:intergenic | 55.0% | |
| GGCCTAGATACCAGGAGTTG+AGG | - | chr7.4:50627352-50627371 | None:intergenic | 55.0% | |
| GTCAGCACATCAGTGGCAGT+TGG | + | chr7.4:50626505-50626524 | MS.gene66866:intron | 55.0% | |
| GTGCCGAGAACCTCAACTCC+TGG | + | chr7.4:50627339-50627358 | MS.gene66866:CDS | 60.0% | |
| TGTGGCTCGCTGCACGACAC+AGG | + | chr7.4:50626357-50626376 | MS.gene66866:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 50626300 | 50627475 | 50626300 | ID=MS.gene66866 |
| chr7.4 | mRNA | 50626300 | 50627475 | 50626300 | ID=MS.gene66866.t1;Parent=MS.gene66866 |
| chr7.4 | exon | 50626300 | 50626378 | 50626300 | ID=MS.gene66866.t1.exon1;Parent=MS.gene66866.t1 |
| chr7.4 | CDS | 50626300 | 50626378 | 50626300 | ID=cds.MS.gene66866.t1;Parent=MS.gene66866.t1 |
| chr7.4 | exon | 50627275 | 50627475 | 50627275 | ID=MS.gene66866.t1.exon2;Parent=MS.gene66866.t1 |
| chr7.4 | CDS | 50627275 | 50627475 | 50627275 | ID=cds.MS.gene66866.t1;Parent=MS.gene66866.t1 |
| Gene Sequence |
| Protein sequence |
