AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67040.t1 MTR_6g033535 98.473 262 2 2 1 262 1 260 0.0 521
MS.gene67040.t1 MTR_6g033535 99.145 234 2 0 29 262 15 248 3.23e-172 477
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67040.t1 AT5G13570 77.481 262 59 0 1 262 1 262 3.39e-151 427
MS.gene67040.t1 AT5G13570 73.818 275 59 1 1 262 1 275 1.37e-147 418

Find 50 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATATGGTGTCTCCCTTTC+TGG 0.137033 6.2:+23621488 MS.gene67040:CDS
AGACCTGATTTGCCACTTAA+AGG 0.227358 6.2:+23621642 MS.gene67040:CDS
AGAATTATTGCCCCAGTTAC+AGG 0.269034 6.2:-23617536 None:intergenic
AATGCAGATCTTTGAAATCT+TGG 0.274871 6.2:+23621589 MS.gene67040:intron
TTGACAGTGTTTGCTAGTTA+AGG 0.284066 6.2:+23618490 MS.gene67040:intron
AAGGTTCGAGTTCCTGTAAC+TGG 0.310426 6.2:+23617524 MS.gene67040:CDS
CACTGGTCTTAAGCTGTATA+TGG 0.326583 6.2:+23621473 MS.gene67040:CDS
ATTTGCCCCACAAACCAAAA+AGG 0.347992 6.2:+23619463 MS.gene67040:CDS
ATGCAGGTCTTGGAAGAAAC+TGG 0.365507 6.2:+23619335 MS.gene67040:intron
ATACCTTTAAGTGGCAAATC+AGG 0.382526 6.2:-23621645 None:intergenic
AATGATTGAAGATCTTCCTT+TGG 0.395909 6.2:-23616511 None:intergenic
CGAAAGGATCTTATTTCTTG+TGG 0.433621 6.2:+23616534 MS.gene67040:CDS
TGACAGTGTTTGCTAGTTAA+GGG 0.433791 6.2:+23618491 MS.gene67040:intron
TTAAGTGGCAAATCAGGTCT+TGG 0.443206 6.2:-23621639 None:intergenic
AGGGTTGGAAAGGTTCAAGT+TGG 0.451483 6.2:+23618510 MS.gene67040:CDS
TCTTGATGAAACCTTTGAAA+GGG 0.457863 6.2:+23617556 MS.gene67040:CDS
GTACGACTCTACATTATTGC+TGG 0.465038 6.2:+23619422 MS.gene67040:CDS
TTCAACACACCAACCTCCTA+TGG 0.469327 6.2:+23621614 MS.gene67040:CDS
GATATCACATCATCACTAGA+AGG 0.473059 6.2:-23621441 None:intergenic
TCATCTATATGGGCCACATA+AGG 0.495540 6.2:-23617476 None:intergenic
TACAGGAACTCGAACCTTGT+AGG 0.496480 6.2:-23617519 None:intergenic
GTGATATCTCGTGGGATCAC+TGG 0.499501 6.2:+23621456 MS.gene67040:CDS
TTGGCACATTTAGCACAAAC+CGG 0.502442 6.2:-23616492 None:intergenic
AGTGTTTGCTAGTTAAGGGT+TGG 0.504029 6.2:+23618495 MS.gene67040:intron
TTCTCAGCTTACCCTTTCAA+AGG 0.507737 6.2:-23617567 None:intergenic
TAAGGACTTCACTTCCTACA+AGG 0.509912 6.2:+23617505 MS.gene67040:CDS
AACTCCTCGATGATCTCTGC+AGG 0.514488 6.2:+23614522 MS.gene67040:CDS
ACATAGGAAATACCTTTAAG+TGG 0.517044 6.2:-23621654 None:intergenic
AGTTGTACTCACGCCAGAAA+GGG 0.521072 6.2:-23621501 None:intergenic
TTGCTAGTTAAGGGTTGGAA+AGG 0.537004 6.2:+23618500 MS.gene67040:CDS
TATCTTTACAGGAAATTGCA+TGG 0.538158 6.2:+23621397 MS.gene67040:intron
AGACTTAAAGACTTCAGAGA+TGG 0.542098 6.2:-23616601 None:intergenic
TTCTTGATGAAACCTTTGAA+AGG 0.543629 6.2:+23617555 MS.gene67040:CDS
AAATCAGGTCTTGGAGCCAT+AGG 0.545316 6.2:-23621630 None:intergenic
AGGTTCGAGTTCCTGTAACT+GGG 0.545714 6.2:+23617525 MS.gene67040:CDS
TCTGAAGTCTTTAAGTCTGA+AGG 0.553893 6.2:+23616606 MS.gene67040:CDS
TGTTTCAAAATTTCTTAACA+AGG 0.554360 6.2:+23619364 MS.gene67040:CDS
AGATCATCGAGGAGTTCTTG+AGG 0.556196 6.2:-23614515 None:intergenic
GTCTTGGAGCCATAGGAGGT+TGG 0.558406 6.2:-23621623 None:intergenic
AAGTTGTACTCACGCCAGAA+AGG 0.559904 6.2:-23621502 None:intergenic
AAGATCTTCAATCATTCGAA+AGG 0.567710 6.2:+23616518 MS.gene67040:CDS
GAGTTCTTGAGGAGGAAGAA+CGG 0.571382 6.2:-23614504 None:intergenic
TTCTTGTGGAGTATGCACAT+TGG 0.597459 6.2:+23616548 MS.gene67040:CDS
TCAGGTCTTGGAGCCATAGG+AGG 0.601393 6.2:-23621627 None:intergenic
GTTTGTGCTAAATGTGCCAA+AGG 0.616660 6.2:+23616495 MS.gene67040:CDS
AGTGATGATGTGATATCTCG+TGG 0.625983 6.2:+23621447 MS.gene67040:CDS
GGTTCGAGTTCCTGTAACTG+GGG 0.643999 6.2:+23617526 MS.gene67040:CDS
TCATCGAGGAGTTCTTGAGG+AGG 0.644982 6.2:-23614512 None:intergenic
GTGATGATGTGATATCTCGT+GGG 0.691549 6.2:+23621448 MS.gene67040:CDS
CGAACCTGCAGAGATCATCG+AGG 0.758435 6.2:-23614526 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr6.2 gene 23614458 23621663 23614458 ID=MS.gene67040
chr6.2 mRNA 23614458 23621663 23614458 ID=MS.gene67040.t1;Parent=MS.gene67040
chr6.2 exon 23614458 23614543 23614458 ID=MS.gene67040.t1.exon1;Parent=MS.gene67040.t1
chr6.2 CDS 23614458 23614543 23614458 ID=cds.MS.gene67040.t1;Parent=MS.gene67040.t1
chr6.2 exon 23616492 23616643 23616492 ID=MS.gene67040.t1.exon2;Parent=MS.gene67040.t1
chr6.2 CDS 23616492 23616643 23616492 ID=cds.MS.gene67040.t1;Parent=MS.gene67040.t1
chr6.2 exon 23617450 23617577 23617450 ID=MS.gene67040.t1.exon3;Parent=MS.gene67040.t1
chr6.2 CDS 23617450 23617577 23617450 ID=cds.MS.gene67040.t1;Parent=MS.gene67040.t1
chr6.2 exon 23618497 23618592 23618497 ID=MS.gene67040.t1.exon4;Parent=MS.gene67040.t1
chr6.2 CDS 23618497 23618592 23618497 ID=cds.MS.gene67040.t1;Parent=MS.gene67040.t1
chr6.2 exon 23619341 23619493 23619341 ID=MS.gene67040.t1.exon5;Parent=MS.gene67040.t1
chr6.2 CDS 23619341 23619493 23619341 ID=cds.MS.gene67040.t1;Parent=MS.gene67040.t1
chr6.2 exon 23621408 23621511 23621408 ID=MS.gene67040.t1.exon6;Parent=MS.gene67040.t1
chr6.2 CDS 23621408 23621511 23621408 ID=cds.MS.gene67040.t1;Parent=MS.gene67040.t1
chr6.2 exon 23621596 23621663 23621596 ID=MS.gene67040.t1.exon7;Parent=MS.gene67040.t1
chr6.2 CDS 23621596 23621663 23621596 ID=cds.MS.gene67040.t1;Parent=MS.gene67040.t1
Gene Sequence

>MS.gene67040

ATGTCAAATCATCATCATCGTTCTCCAAGTTCTTCCTACTACAAAACCGTTCTTCCTCCTCAAGAACTCCTCGATGATCTCTGCAGGTTCGTTCATCAATTTTCCCTTTTTCTGCTTTTAGGGTTACGTTCTTCTGAATTTTTACTTGCTTATAAATGATTATGCACAATAACTTATGCAAATAAATAAGCTTTTATGTATTATTCATAAGCTTGTCAATGTAGTTTTTGAAAAAACAGCTTATGAATTAACATAAAAAGCTTATTTATTTGCATAAGCTATTTTTCATAAGCTTAAAAATAATTCATTCCAAATGGGCCCTAATTGTGAGTTGCATATGAAGTGGTTTGTGTTTGGATATATATATGTATAAGTGAGTTAAACGATATATTGTTTCAATTTAGAATCAATTCTACTAGAGAACATCTAAGTGTATGCTTGGTTCCACTTTTGAGGAATCCAAAGTTTATTCTGGAGGTGTAAAATTGATTTTGACATAATTGATTGTTTTCAAGAAGAATTGATTTTGCCTCTAGAATTGATTCTACTTGAAGCTATGATTTGTAGCTTTTGATTCTAGAATTGATTTTAACACTCAAGTTTATTGTTCAAGTCACTTTTACGTTTAGATTCAATTTTACAAAACCAATTAACTCAAATTCAATTATTTTCACCATAGAACCAAACACACAGTAAACATGTCAAAATCAATTCCACACCTCCGAAATCATTGATTCCTCGTGAAACCAAACATACACTTAGAAATTGACTAGTGCTGTCTTACTTGAGGAATTGCGGTGTTGGCAATGGATGAAACATTTATTATATTTTTTAAAATGAACAACTATTAAGAAGATGATAGATTAAGCATCGATGATTCAATTCATGGTTCCTGAATATGTGAAGTGCTGTTACTTTAGTCCTGAGTGTATCAAAGTTACAAAGGCATCCTCAAGTGTCGCTTTTGTTAGTAATTTTATGGACTTGCAAAAAATGCAGTTAAAGAAGAATTTAGGCATGTTGTTGTTGTTGTGATTTTGATACATTCAAATACTATCATATTCATGACAGAGCCTCTTGCATTTATGGACCAAAATGACTGTTTGCTCTTATTATCAATTTATTTTACTGTATACTTATTAGTCGAGCTGTTAAAGGGTTTTTAATTGCCGAAGAATAGATTACTAGGCTACTTTTTTTTTTGGTTAAAATCTAATTGTTAGGATTCTGATACTCAATCTGCAATAAGAAAACGAACACTGCGAAAATAAACCATATTGAGTATAAGACGAAGATACTAGTAATAATTGCCTTGAGCATACAATATAAAATGAGCCTCATTAATAAACACATTTTTCTCTAAGGAGGGAAATTTGGAAATGACTGAACTATCTATGGAAACTGTATTAAGTTAAATGAAAAGAAAGAATAACTAATCACTATAAATACAATCGACAGTGAGATGGCTGAAGCCAAAGTTATTTCATCGCCTATAGTTTCTGGCTGCAAGTTGTCAAAACAGGGCGATGATGCTATACAGAGGGAACCTCCAAATGCCAAATCACTAGACCTGGAATCAACTTACTTAGTTAACAAGTCAACTTATATCCTTCCATATGGCTATTGGCGTAGAAGTACGATTACCGTATGTACTTCGATGGAAGAAATATCTTAAGAAAAATCAGGTTTATTCATACGAATCAACAACATCTAGAATAATGTCAGAAAGAGATTAATAACATTTAGAGAGGATTAATAAGGGAGTGGTTTAGAATATACCTTGTTTTCTGGGGTTCTGTCGCCCATTCAATTGTTTTATTCGGTTTTTATTTGTACTTTCTGATAATAGCTTGGACATTTGTTGAAATGATTTGGTATGCATTTTTGGTGATATTGGTTTATCGTATGCTGTTTGATATTCTATCCTTTTATTTGTGTCACACTCGGAATCATTTCCATTGACTCTCTCCTCTCTATTTCCTTTATTTACGCTGTTCTTATAGTAAAAGGCCACTTTGTGTGTTTATATTGCAGCCGGTTTGTGCTAAATGTGCCAAAGGAAGATCTTCAATCATTCGAAAGGATCTTATTTCTTGTGGAGTATGCACATTGGTTTTATGAAGATAATTCTGTAGAAAATAATCCATCTCTGAAGTCTTTAAGTCTGAAGGAGTTCACTTCTTTAAGTATCCTTACAGCAGTTTTCTTTTGTTTTCTATCCTCTGTTTAAAATAGCATAAGTTACTCTTATTACTTAATCTACATTTTATAACAGTAATATATGAGCTATTCTAATTCTTTCATTCACTGGATTCTTTCTCAGTTTTCATTAAACAAGGGAAAACAACATGGTAAGCTGAATTCTTTGGTTTAGACATGAACGTGATATATCCATAGAATGTTTTTTCCTTGGATGTTTTGAAATCTTTGATTTTGTTAAAACTTTGTTGCTCTTCAAGATTTAAATATTCTATTATACTTTATACTTCATACAGTCTCTTGTAATGTATGAAACAAATCGTGGTGGGAATATACACTATTCTCCTCGTATGATTTTCTCTAATATTCTAATACTGAACCAAGCATAACCATGTACAGTTTAGTTTTGTGGAAAGCTTCGTATTTTTGTCGTCATTTATGCCATGTTTAAGGTTGGAAACTGCATGTGTTACATGGATTTCAGATCAAACTATTAGTGGCTTAGTGGAAATTTTCTCTGCATGGTATTGAACAGAAAGATGCTCACATATTTGTGCAATGATACACACATACTTCAAATGAAATGCTAATATTAGAATAGTGTTGTTTTTGAATTTAATTTTTTGACCAGGCGAGACGAGGATATCCCTGTTATGATTTACTTATTAGACATTTGAAGGCAATGTTGTCTAGGCTTTGCATAGAAAAGGCTAATCATCCCGGCATTTTAATTTCATTGTCCTAATCTCTGTATTCACATCTTATACCTGGATTCTTCCTTAATATTGATCAGTGTTCAATAGCTGTGATGTTTTGAAGCCTTATGTGGCCCATATAGATGATATTTTTAAGGACTTCACTTCCTACAAGGTTCGAGTTCCTGTAACTGGGGCAATAATTCTTGATGAAACCTTTGAAAGGGTAAGCTGAGAATAAACGCTCTTAACAGTACTGATTATTTGGTTTCTTCATAAGACAATACCTTGTTTCTCTATTCTATTACAATTTATTATTATGGGTTATTTTTTGAAATATCGCTTTCATCTCCACAAATCCTGTGTTCCATAAAACCTACTGTTTCTGAACATGAGGGATGTAGGTACTTTAAATGTTCATTAAATTTTTTTAATTAGGTCTCTCAGAAGCAAAAATTTTCTGACTCAAGCACAGCTATTTTATTTTTTTGACTCGAGCACAATTATTTATGAAGAAAAGTCATTCAGGTTTATTTAGTTTATAACCTGCAATTTCAAGCTGTCACATTTTCAGAAGGGGAAAGCCATATGTTGAAGCTAGTGTATATTTTACATCATGTCATAAATTTTTCTAGGTTCAAGAAAATAATTTTATAATGAAGCCATGCAACTTTTCTAGGGAACTGTTAGACTTGTGGTTTATATTCTGTCAAGCAGTTATTTTAGGACAGTTATGTTTTGTCTTATCTATGTTGTGTCAGTTATGTTTTATTAATTAATTATTAATTGAGTCAGTTATGTTTTAAGGATCAATAAGAGAAGGGTCAGACATAAGAGGGTTGTCTGGAAGTTATTGAGTGGTTATTGGGATTTGGGAGAGACCCAACTCTCAAACATTTGAGGAAGGGAGAGGCCAAGGCTCTCGAAACCTTGGAGAACAATTATCCTAGTGTTATTATGTGTGATTTTTGTGTTATTCTCTATACTGTAATTCTGTTTGTATCAATAAATATCAGAAATTGTTCCAAATCTGTTTGTTATTGAACCAATATCTATCAGAAACCTCCATTGCGGGACTCAAAATGTTCCTATCTAAGTTATTTATTGGTATGGTGATGCTTATTTACTTCCATATCTGTTGACAGTGTTTGCTAGTTAAGGGTTGGAAAGGTTCAAGTTGGAGTTTTCCTCGTGGCAAAAAGAGTAAAGATGAAGAAGATCATGCATGTGCTGTTAGAGAAGTACGCGTGACTGTTCAGAGTTATCCCTTTTTGATTCTAGTCGTGAATGACTCTCTTTCCATTTTCTTTTGCCCTGTTGGTCTAGGAAACATAACAAAATTGATATAGATTTATAAAACCTACAAGTATTTTGAATTGGCTAATTCATGTCATACTAAGGGTGAATTTCACCCATCTTAGTTTTGGCTTCTCATCTCCTTAAATGGAGAAGTTAGGCCAAAAGTCCTTCTTAAAGACTAGTAGGCATATTGAAACAATCCTCACATATGTTATCTATTAAATGGTTTTACATACCGATAATTTTTATTTATTTCAATAACATAACAGACTATTTTTTAAATTATTTAAAATTTACATGTACGATCTATGTGATAGTTATTCTTAATACAACAGTGAGTTTTGTAATACAAGCATTTGATAAAAAGTTATTGCACAGCGTAAAAATATCATTGTATGTTGGTTTAATTTTCACACACACACACACACACACATTCTAAAAGAAATTGAAAACTATTACTTGCTAATGTTGGTCTTATAGAATTTTTAATAAGTTTGAGGTTTTTATAAAAATATTTTACCAAAAATTTTTATTTAAAAAATAACCAGTAAGCTGGAATCAAGCTGAAATCACCAAACAATTTCAGCTCTTATTTTTAGTTTTTAATTTAAGGAAAATTTAAGCTTGGTCAAATACACCAGAGTCTTGCTTTTGCTTTTCCTAACTTGTTTTTGTTTATGTTAAATGCAGGTCTTGGAAGAAACTGGTTTTGATGTTTCAAAATTTCTTAACAAGGATGAATACCTTGAAGTTATTTTTGGACAACAGAGAGTACGACTCTACATTATTGCTGGTGTCAAAGATGATACTTCATTTGCCCCACAAACCAAAAAGGAGATCAGTGTATGTAATTCTTTTGATGTATTTATTTTGAAGGTTTTTATAATTCAATCATCGTATTTCCTTAACACCAGATTTATCACCAATATATTTAGTAGTAGTTTCGGATAAAAAAATAAAATTTCTTTACATAAAGAGAACGGGAATTTACAAGGCACTATGAGTTATACCCTGATTTCCAAAACAAAGAAGCTAGACGGAAAACATAGAGTCACAGTACTCCAAAAACAAGAAAACATCTGCATATAAGGAGAACATTGTTGAAATTTGGGGCCTAACTCATCCTTACAAAACCGGCTTGCAAGGTGAGGATTGCCTCCTCTTTATATACTATTCTCAAGAGTCTTGTCTATCCGATGTGGGACTTGGGTTTTTCCCAATACACCCCCCTCACGCCCAGCACTCTTTGGGCTTGGTGCGTGAATAGGTTTAGGCGGCCCTTGAATTCGACAAGCTCTGATACCATGTTGAAATTTGGGGCCTAACTCATCCTTACAAAACCGGCTTGCAAGGTGAGGATTGCCTCCTCTTTATATACTATTCTCAAGAGTCTTGTCTATCCGATGTGGGACTTGGGTTTTTCCCAATAAACATATATGATCATGGGTATTGGGTATTCTCCTCCAGCCTAATGTACATATTATTCTGTTAGGATATAGGATGAAGGAGAATTAGTTAGAGAAGTTTGTTATTTGATTAGTTGGAGAGTATCATTTATAGATAGAAGAATACAAGGACATAGAAGATTCATCGAGTATTGTAAACATTTTGTAAATAGAGTAGTGACTGTGACTCTATTGTAGAAGGGGATAGTTTTCTCTCCAATTCTTTCATATTTTCTCGATTAAAGTGTTCTTAGTATTCAATTCTAATTTTCAGGTTTGTAACATATTCTCTTTTAAACTGCCCCACAGATGGATATAGGATAAAGAGGGAGATTACTAGGATATGTAAACATAATCAAAATCTTATTACCTCTTTACAACAAGTGTATCATATCTAAGATTAAAGTTTCCAATTTCTGAAATTAAAATATCAAATTATTATTTAACAAAAACACACTTAGTACAGAGATAATTAAGATTTTTTTTTTTTTGTTTTTGGCAAAACAAAATTTAAAACATTGAAAGCTTTATATTTAATCACGCCGGCAACACACTCCATCAAACCAAGAGTTGCATGTGTAGGCTCTAAAAGGTTGATAAATGCGGGTTTCCCAAAAACACTCGTTCTTGCCTAAGACTAGACATCTAGAGAGTGGGAAAATATGGTTTGCTCAATAACATACATAGGATAGTCCGATGCATATTTTAAAGTTTATACTTAGCGTAATTCAACCCGAAAAGCTCGAGGGATGTGGATTGCCCAAGTCTATCGGAATTACTTTGACCATATTTCAAGAAGGTTAACATTTTTCTATTTTGTCACCAAACCCTTTATATTGAATAAATGAAGTTGTGAAACATATAGTACTTAATCATCTTTATCCCTTGTTCAGCTGTAGTTTGTGAACTAGTGTTGCCGATATATTGGAGTCACGTTTCCTTGAAATTTGAATTTGTATTTATCCCCTGCATTGTTTAGTTTTATAGCTCCTTAGCTACTGTAGGAAATAAGAATTATAAGAGAAGTAGTTAGACTAGCTTAGTTATAGTTCTACTATAGGAGATAAGAATTATAAGAGAAGTAGTTAGACTAGCTTAGTTATAGTTAGATCAGTGTGCTACAGCTGGCACCAAACAGCTATGAACAGTTGTCAAGTGACGGACACATTTCAGTTTGTAAAAGGTTATTGTAACTGTTTTCTATTCATTCAATGAGAGTCAGAAATTGATAAGAAAAGTTGGTTAAAAATCTTCTATGAACTCTTCTGAATTTTACAAGAAGCTGAGAATTCATCTTGTTATATCTTTACAGGAAATTGCATGGCAACGTCTTGATGAACTTCAACCTTCTAGTGATGATGTGATATCTCGTGGGATCACTGGTCTTAAGCTGTATATGGTGTCTCCCTTTCTGGCGTGAGTACAACTTTATGTTTTTGATATTTTGGTAATGTCTCTTGTGTTGTCAATGTTGAAAAAAATTTCACTGCCTTAATGCAGATCTTTGAAATCTTGGATTTCAACACACCAACCTCCTATGGCTCCAAGACCTGATTTGCCACTTAAAG
Protein sequence

>MS.gene67040.t1

MSNHHHRSPSSSYYKTVLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSLSLKEFTSLMFNSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWKGSSWSFPRGKKSKDEEDHACAVREVLEETGFDVSKFLNKDEYLEVIFGQQRVRLYIIAGVKDDTSFAPQTKKEISEIAWQRLDELQPSSDDVISRGITGLKLYMVSPFLASLKSWISTHQPPMAPRPDLPLK