AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67187

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67187.t1 MTR_1g074880 98.462 325 5 0 1 325 1 325 0.0 666
MS.gene67187.t1 MTR_1g074880 98.428 318 5 0 1 318 1 318 0.0 652
MS.gene67187.t1 MTR_1g043240 83.129 326 46 2 1 325 1 318 0.0 571
MS.gene67187.t1 MTR_1g079950 80.615 325 63 0 1 325 30 354 0.0 571
MS.gene67187.t1 MTR_1g079950 80.615 325 63 0 1 325 1 325 0.0 570
MS.gene67187.t1 MTR_7g116380 81.846 325 59 0 1 325 1 325 0.0 570
MS.gene67187.t1 MTR_1g079950 81.040 327 59 2 1 324 1 327 0.0 568
MS.gene67187.t1 MTR_1g079950 82.132 319 56 1 1 318 1 319 0.0 566
MS.gene67187.t1 MTR_7g116380 83.072 319 53 1 1 318 1 319 0.0 564
MS.gene67187.t1 MTR_1g074880 98.127 267 5 0 59 325 4 270 0.0 551
MS.gene67187.t1 MTR_1g074880 98.077 260 5 0 59 318 4 263 0.0 536
MS.gene67187.t1 MTR_5g081230 81.457 302 55 1 1 301 17 318 0.0 525
MS.gene67187.t1 MTR_8g098980 74.132 317 81 1 1 316 1 317 0.0 513
MS.gene67187.t1 MTR_8g005010 77.241 290 66 0 6 295 4 293 3.45e-177 492
MS.gene67187.t1 MTR_7g112870 73.701 308 75 2 1 308 5 306 7.54e-173 482
MS.gene67187.t1 MTR_1g079950 82.463 268 44 2 60 324 34 301 5.27e-171 477
MS.gene67187.t1 MTR_5g093990 46.758 293 150 3 26 317 17 304 2.75e-94 282
MS.gene67187.t1 MTR_7g107310 48.175 274 140 2 26 298 27 299 3.30e-94 282
MS.gene67187.t1 MTR_8g062430 48.175 274 140 2 26 298 20 292 8.36e-93 278
MS.gene67187.t1 MTR_8g085610 45.455 297 153 3 3 298 6 294 2.79e-92 277
MS.gene67187.t1 MTR_5g037200 48.175 274 140 2 26 298 20 292 1.80e-91 275
MS.gene67187.t1 MTR_8g085610 47.037 270 141 2 30 298 2 270 5.67e-90 270
MS.gene67187.t1 MTR_1g039380 45.247 263 141 3 25 285 15 276 2.24e-89 270
MS.gene67187.t1 MTR_1g039380 45.783 249 132 3 39 285 5 252 4.60e-87 263
MS.gene67187.t1 MTR_4g050540 47.541 305 141 8 9 295 649 952 6.40e-83 270
MS.gene67187.t1 MTR_2g087530 47.541 305 141 8 9 295 644 947 7.57e-83 270
MS.gene67187.t1 MTR_1g089370 46.774 310 148 6 3 295 522 831 9.26e-83 268
MS.gene67187.t1 MTR_2g087530 46.688 317 148 9 9 307 531 844 6.53e-82 266
MS.gene67187.t1 MTR_3g452710 45.059 253 132 4 33 279 195 446 7.13e-73 233
MS.gene67187.t1 MTR_3g452710 45.059 253 132 4 33 279 262 513 2.70e-72 233
MS.gene67187.t1 MTR_2g100130 40.484 289 160 6 22 299 102 389 6.58e-69 220
MS.gene67187.t1 MTR_2g100130 42.642 265 144 5 22 279 201 464 1.10e-67 220
MS.gene67187.t1 MTR_2g100130 42.642 265 144 5 22 279 211 474 1.15e-67 220
MS.gene67187.t1 MTR_2g100130 42.642 265 144 5 22 279 172 435 1.21e-67 219
MS.gene67187.t1 MTR_2g100130 42.642 265 144 5 22 279 211 474 1.53e-67 220
MS.gene67187.t1 MTR_2g100130 42.642 265 143 6 22 279 211 473 2.89e-66 217
MS.gene67187.t1 MTR_2g100130 42.642 265 143 6 22 279 172 434 2.92e-66 216
MS.gene67187.t1 MTR_2g100130 42.529 261 142 5 22 275 211 470 7.83e-66 215
MS.gene67187.t1 MTR_2g100160 40.288 278 154 6 33 299 132 408 1.24e-61 202
MS.gene67187.t1 MTR_5g037200 50.898 167 81 1 26 191 20 186 1.38e-56 183
MS.gene67187.t1 MTR_8g072600 31.746 315 148 10 33 281 100 413 1.31e-38 142
MS.gene67187.t1 MTR_8g072600 32.661 248 121 7 33 235 100 346 7.28e-32 122
MS.gene67187.t1 MTR_2g099710 26.923 286 137 11 31 268 211 472 4.52e-20 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67187.t1 AT2G39840 79.412 306 62 1 1 305 14 319 0.0 527
MS.gene67187.t1 AT5G43380 77.389 314 71 0 1 314 1 314 0.0 523
MS.gene67187.t1 AT5G43380 77.389 314 71 0 1 314 1 314 0.0 521
MS.gene67187.t1 AT5G43380 77.389 314 71 0 1 314 1 314 0.0 521
MS.gene67187.t1 AT1G64040 75.710 317 76 1 1 316 1 317 0.0 518
MS.gene67187.t1 AT2G29400 80.263 304 56 3 1 301 15 317 0.0 518
MS.gene67187.t1 AT5G59160 78.878 303 62 2 1 301 9 311 0.0 515
MS.gene67187.t1 AT5G59160 78.878 303 62 2 1 301 9 311 0.0 515
MS.gene67187.t1 AT5G59160 78.878 303 62 2 1 301 9 311 0.0 515
MS.gene67187.t1 AT3G46820 76.898 303 68 1 1 301 9 311 0.0 505
MS.gene67187.t1 AT4G11240 71.293 317 90 1 1 316 1 317 2.78e-178 496
MS.gene67187.t1 AT5G27840 75.850 294 69 1 1 294 9 300 9.16e-174 484
MS.gene67187.t1 AT5G27840 75.850 294 69 1 1 294 9 300 1.20e-173 484
MS.gene67187.t1 AT5G27840 75.850 294 69 1 1 294 9 300 1.28e-173 484
MS.gene67187.t1 AT5G27840 75.850 294 69 1 1 294 9 300 1.28e-173 484
MS.gene67187.t1 AT3G05580 75.850 294 69 1 1 294 9 300 1.22e-171 479
MS.gene67187.t1 AT3G05580 75.203 246 59 1 1 246 9 252 1.59e-139 395
MS.gene67187.t1 AT5G55260 47.099 293 149 3 26 317 17 304 9.30e-97 288
MS.gene67187.t1 AT2G42500 48.540 274 139 2 26 298 27 299 2.02e-95 285
MS.gene67187.t1 AT3G58500 47.810 274 141 2 26 298 27 299 5.17e-94 282
MS.gene67187.t1 AT1G69960 46.284 296 150 3 4 298 6 293 2.65e-93 280
MS.gene67187.t1 AT4G26720 45.230 283 146 2 26 299 17 299 1.03e-92 278
MS.gene67187.t1 AT1G10430 45.608 296 152 3 4 298 5 292 4.57e-92 276
MS.gene67187.t1 AT1G10430 45.608 296 152 3 4 298 5 292 4.57e-92 276
MS.gene67187.t1 AT1G59830 46.284 296 150 3 4 298 5 292 6.34e-92 276
MS.gene67187.t1 AT3G19980 45.247 263 141 3 25 285 15 276 4.46e-89 269
MS.gene67187.t1 AT1G50370 44.867 263 142 3 25 285 15 276 6.25e-89 268
MS.gene67187.t1 AT5G55260 41.317 334 149 4 26 317 17 345 4.92e-88 268
MS.gene67187.t1 AT4G03080 46.452 310 149 7 3 295 524 833 2.76e-83 270
MS.gene67187.t1 AT2G27210 46.885 305 143 8 9 295 650 953 1.79e-81 266
MS.gene67187.t1 AT2G27210 46.885 305 143 8 9 295 655 958 1.89e-81 266
MS.gene67187.t1 AT1G59830 49.004 251 119 3 4 253 5 247 6.42e-81 246
MS.gene67187.t1 AT1G08420 46.885 305 143 8 9 295 661 964 9.44e-81 265
MS.gene67187.t1 AT1G08420 46.885 305 143 8 9 295 666 969 9.95e-81 265
MS.gene67187.t1 AT1G03445 46.479 284 135 7 26 294 476 757 9.67e-79 256
MS.gene67187.t1 AT1G03445 46.479 284 135 7 26 294 476 757 1.09e-78 256
MS.gene67187.t1 AT2G42500 49.321 221 110 2 79 298 33 252 4.34e-75 232
MS.gene67187.t1 AT2G42810 44.664 253 133 4 33 279 197 448 3.85e-70 226
MS.gene67187.t1 AT2G42810 44.664 253 133 4 33 279 197 448 3.85e-70 226
MS.gene67187.t1 AT2G42810 44.664 253 133 4 33 279 197 448 3.85e-70 226
MS.gene67187.t1 AT2G42810 44.664 253 133 4 33 279 251 502 9.52e-70 226
MS.gene67187.t1 AT2G42810 44.664 253 133 4 33 279 250 501 1.19e-69 226
MS.gene67187.t1 AT2G42500 48.299 147 72 3 26 168 27 173 2.81e-44 150
MS.gene67187.t1 AT2G42500 47.403 154 75 4 26 175 27 178 8.62e-44 149
MS.gene67187.t1 AT2G42500 50.360 139 65 3 26 160 27 165 1.36e-43 148
MS.gene67187.t1 AT5G63870 30.473 338 168 11 33 303 52 389 5.25e-36 134
MS.gene67187.t1 AT5G63870 30.473 338 168 11 33 303 52 389 5.25e-36 134
MS.gene67187.t1 AT1G48120 29.706 340 167 9 31 300 628 965 3.19e-30 121
MS.gene67187.t1 AT5G63870 31.837 245 127 6 33 237 52 296 3.53e-29 114
MS.gene67187.t1 AT5G10900 33.491 212 117 8 55 245 225 433 1.41e-22 98.6
MS.gene67187.t1 AT5G10900 33.491 212 117 8 55 245 225 433 1.55e-22 98.6
MS.gene67187.t1 AT5G10900 33.491 212 117 8 55 245 225 433 1.65e-22 98.6

Find 55 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATTCTCCACAGTAATT+AGG 0.241896 1.2:-57595213 None:intergenic
AAATCCGTAGATACGGTTTA+TGG 0.280200 1.2:-57594691 None:intergenic
AATATACCACAGATCTTAAT+AGG 0.298021 1.2:-57593114 None:intergenic
GTCTATTATGTGACCTCTTA+TGG 0.298995 1.2:+57594901 MS.gene67187:CDS
TGATTTAAATCGTGTAATTC+AGG 0.302882 1.2:-57594837 None:intergenic
TGTGGAGAATTTGACAATTC+TGG 0.322802 1.2:+57595222 MS.gene67187:CDS
CATATACATTTGGTGCTGAT+AGG 0.345062 1.2:+57594976 MS.gene67187:CDS
CGCGGAGTTTCATATACATT+TGG 0.347483 1.2:+57594966 MS.gene67187:CDS
GGAGATACAATGTGAAACTA+TGG 0.351983 1.2:+57594730 MS.gene67187:CDS
ATGATCTTGATTTGATTTGT+AGG 0.380978 1.2:+57595021 MS.gene67187:CDS
TAGTAGTGATGTTCGTGGTT+GGG 0.384770 1.2:+57594932 MS.gene67187:CDS
AGAAACAATATGTCTTCTTC+TGG 0.391096 1.2:+57594608 MS.gene67187:CDS
ATTCGTGGTTACCCCTTAGA+AGG 0.394389 1.2:-57594662 None:intergenic
TTTGATTTGTAGGGCTCATC+AGG 0.400695 1.2:+57595031 MS.gene67187:CDS
TTGTTGATGGTATTATCAAT+AGG 0.402079 1.2:+57592968 MS.gene67187:CDS
TCACATAATAGACCGGATTC+AGG 0.421205 1.2:-57594891 None:intergenic
GAAAGCTGAACCTGCTTCCC+TGG 0.443268 1.2:-57593012 None:intergenic
AGTAGTGATGTTCGTGGTTG+GGG 0.445269 1.2:+57594933 MS.gene67187:CDS
ATGGAACGTGGAGTTGTTGA+TGG 0.454585 1.2:+57592955 MS.gene67187:CDS
AAGAGGAAGACCAGGGAAGC+AGG 0.459465 1.2:+57593002 MS.gene67187:CDS
ATGTTACCAGGAGATATTCA+TGG 0.467260 1.2:+57594483 MS.gene67187:intron
TGATCTTGATTTGATTTGTA+GGG 0.484836 1.2:+57595022 MS.gene67187:CDS
CAACCACGAACATCACTACT+AGG 0.486128 1.2:-57594930 None:intergenic
ATATACATTTGGTGCTGATA+GGG 0.489717 1.2:+57594977 MS.gene67187:CDS
CTAGTTTCAACAGATTAGGC+TGG 0.491885 1.2:-57593086 None:intergenic
TAGTAGTCCAACAAAATTCA+AGG 0.492608 1.2:+57595350 MS.gene67187:CDS
GCCTCTAGTTTCAACAGATT+AGG 0.495023 1.2:-57593090 None:intergenic
AATAGGTTACTTGAAGTAAG+AGG 0.499906 1.2:+57592985 MS.gene67187:CDS
CTAGTAGTGATGTTCGTGGT+TGG 0.504572 1.2:+57594931 MS.gene67187:CDS
CCGTGTGAAGTTCCTGAATC+CGG 0.507419 1.2:+57594879 MS.gene67187:CDS
TACTAGGATCAGACCATAAG+AGG 0.507611 1.2:-57594914 None:intergenic
GTCAATATTCCGACCTTCTG+AGG 0.518139 1.2:+57594505 MS.gene67187:CDS
CTTGAAGTAAGAGGAAGACC+AGG 0.522885 1.2:+57592994 MS.gene67187:CDS
CATCATAAAATCCGTAGATA+CGG 0.530362 1.2:-57594698 None:intergenic
TTTCAACTGTTTGCCTGTGG+CGG 0.542655 1.2:+57594770 MS.gene67187:CDS
GTGCTTCCAAAGCTAAACTT+AGG 0.544982 1.2:-57595312 None:intergenic
ATGTTGCAGGTTGTGGAAGA+CGG 0.545369 1.2:+57595144 MS.gene67187:intron
CTCGAAAAGCCTCAGAAGGT+CGG 0.552388 1.2:-57594514 None:intergenic
GGTTTATGGAAGCACATTCG+TGG 0.557667 1.2:-57594677 None:intergenic
GCTTCCATAAACCGTATCTA+CGG 0.579276 1.2:+57594687 MS.gene67187:CDS
GATCCTAGTAGTGATGTTCG+TGG 0.596697 1.2:+57594927 MS.gene67187:CDS
TCATCAATGAGAGCCGCCAC+AGG 0.602243 1.2:-57594783 None:intergenic
TAAGAGGTCACATAATAGAC+CGG 0.605224 1.2:-57594898 None:intergenic
GAAAAGATTATCTGTATGCA+TGG 0.619510 1.2:+57594804 MS.gene67187:CDS
GCCTAATCTGTTGAAACTAG+AGG 0.625183 1.2:+57593089 MS.gene67187:CDS
TTGTTTCAACTGTTTGCCTG+TGG 0.632527 1.2:+57594767 MS.gene67187:CDS
CAAATTAGAAATATGGAACG+TGG 0.634209 1.2:+57592943 None:intergenic
AATAAATAATTCGAGCGAGG+AGG 0.639135 1.2:-57594549 None:intergenic
GAGGCACCTATTAAGATCTG+TGG 0.649585 1.2:+57593108 MS.gene67187:CDS
CGTACTCGAAAAGCCTCAGA+AGG 0.657790 1.2:-57594518 None:intergenic
TTAGGAGACTATGTTGATCG+CGG 0.661259 1.2:+57594573 MS.gene67187:CDS
CCGGATTCAGGAACTTCACA+CGG 0.661978 1.2:-57594879 None:intergenic
TTGAAGTAAGAGGAAGACCA+GGG 0.672507 1.2:+57592995 MS.gene67187:CDS
GGTTGGGGAGAAAGTGAGCG+CGG 0.691158 1.2:+57594948 MS.gene67187:CDS
AAGATTATCTGTATGCATGG+TGG 0.720136 1.2:+57594807 MS.gene67187:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTGATAAAAAAATTATATA+TGG + chr1.2:57593492-57593511 MS.gene67187:intron 10.0%
!!! TTAATTGATTTAATTAGTAT+AGG + chr1.2:57593169-57593188 MS.gene67187:intron 10.0%
!!! TTTAATTAAGATTAATTTTG+AGG + chr1.2:57594286-57594305 MS.gene67187:intron 10.0%
!! AAAAAAATTATATATGGTCT+AGG + chr1.2:57593498-57593517 MS.gene67187:intron 15.0%
!! AAAAAATTATATATGGTCTA+GGG + chr1.2:57593499-57593518 MS.gene67187:intron 15.0%
!! AAATATGATACTAGAAAATT+TGG - chr1.2:57594402-57594421 None:intergenic 15.0%
!! TCATACAAAGTAATTATATA+TGG + chr1.2:57593468-57593487 MS.gene67187:intron 15.0%
!!! ATAAAGTTGTTTTAGATTAT+AGG + chr1.2:57594010-57594029 MS.gene67187:intron 15.0%
!! CAAAAATTATATATGGTCTA+GGG + chr1.2:57593719-57593738 MS.gene67187:intron 20.0%
!! GTATGTATAAACTTAGAATT+TGG - chr1.2:57595100-57595119 None:intergenic 20.0%
!! TGAAACAAGAATGAATAATT+AGG - chr1.2:57594205-57594224 None:intergenic 20.0%
!! TTCTAAGTTTATACATACTA+AGG + chr1.2:57595102-57595121 MS.gene67187:intron 20.0%
!!! AAGACATAGTTTCTTTTTTT+GGG + chr1.2:57593656-57593675 MS.gene67187:intron 20.0%
!!! AGGATTTAATTTGTATACAT+CGG + chr1.2:57593189-57593208 MS.gene67187:intron 20.0%
!!! CTAGACCATATATAATTTTT+GGG - chr1.2:57593500-57593519 None:intergenic 20.0%
!!! GATTTTATGATGAATGTAAA+AGG + chr1.2:57594709-57594728 MS.gene67187:CDS 20.0%
!!! TAAAAACTTTTTCCTTCTAA+GGG + chr1.2:57594650-57594669 MS.gene67187:CDS 20.0%
!!! TCAGTATTTTCTATATTTGT+CGG + chr1.2:57593857-57593876 MS.gene67187:intron 20.0%
!!! TCTTAACTTGTTTTAATGTT+AGG - chr1.2:57593431-57593450 None:intergenic 20.0%
! AAAAATAAATAATTCGAGCG+AGG - chr1.2:57594555-57594574 None:intergenic 25.0%
! AATATACCACAGATCTTAAT+AGG - chr1.2:57593117-57593136 None:intergenic 25.0%
! AGGTGAAATATCAAATATGT+TGG - chr1.2:57594346-57594365 None:intergenic 25.0%
! ATGAAACAAGACATTAGAAT+AGG - chr1.2:57594457-57594476 None:intergenic 25.0%
! CAAGACCCAAAAATTATATA+TGG + chr1.2:57593712-57593731 MS.gene67187:intron 25.0%
! CAATTCTAATTAAACTGATG+TGG + chr1.2:57594160-57594179 MS.gene67187:intron 25.0%
! CATCAGTTTAATTAGAATTG+TGG - chr1.2:57594160-57594179 None:intergenic 25.0%
! CCAAAAATTATATATGGTCT+AGG + chr1.2:57593718-57593737 MS.gene67187:intron 25.0%
! CTCATTATTAAACTATGTGT+GGG + chr1.2:57593397-57593416 MS.gene67187:intron 25.0%
! TCTCATTATTAAACTATGTG+TGG + chr1.2:57593396-57593415 MS.gene67187:intron 25.0%
! TGATTTAAATCGTGTAATTC+AGG - chr1.2:57594840-57594859 None:intergenic 25.0%
!! CAAAATACCTTGAATTTTGT+TGG - chr1.2:57595360-57595379 None:intergenic 25.0%
!! CAGTATTTTCTATATTTGTC+GGG + chr1.2:57593858-57593877 MS.gene67187:intron 25.0%
!! CCTAGACCATATATAATTTT+TGG - chr1.2:57593501-57593520 None:intergenic 25.0%
!! CTTAGAAGGAAAAAGTTTTT+AGG - chr1.2:57594651-57594670 None:intergenic 25.0%
!! TAAAGTAGCAATTTTTGCAT+TGG + chr1.2:57593588-57593607 MS.gene67187:intron 25.0%
!! TAATTTGTATACATCGGTAA+AGG + chr1.2:57593195-57593214 MS.gene67187:intron 25.0%
!! TATTGTTGTTGTTGTTGTTA+TGG + chr1.2:57594045-57594064 MS.gene67187:intron 25.0%
!! TGTAATTTTCTTTATGTTGC+AGG + chr1.2:57595131-57595150 MS.gene67187:intron 25.0%
!! TTTTTGCATTGGAAATTGTA+CGG + chr1.2:57593599-57593618 MS.gene67187:intron 25.0%
!!! AAAAACTTTTTCCTTCTAAG+GGG + chr1.2:57594651-57594670 MS.gene67187:CDS 25.0%
!!! ATGATCTTGATTTGATTTGT+AGG + chr1.2:57595021-57595040 MS.gene67187:CDS 25.0%
!!! CAAGACATAGTTTCTTTTTT+TGG + chr1.2:57593655-57593674 MS.gene67187:intron 25.0%
!!! CGCTCGAATTATTTATTTTT+AGG + chr1.2:57594555-57594574 MS.gene67187:CDS 25.0%
!!! CTAAAAACTTTTTCCTTCTA+AGG + chr1.2:57594649-57594668 MS.gene67187:CDS 25.0%
!!! TCATCGTAACAAGTTTTATA+CGG - chr1.2:57593358-57593377 None:intergenic 25.0%
!!! TGATCTTGATTTGATTTGTA+GGG + chr1.2:57595022-57595041 MS.gene67187:CDS 25.0%
!!! TGTTTTAGATTATAGGAAGT+GGG + chr1.2:57594017-57594036 MS.gene67187:intron 25.0%
!!! TTGTTGATGGTATTATCAAT+AGG + chr1.2:57592968-57592987 MS.gene67187:CDS 25.0%
!!! TTGTTTTAGATTATAGGAAG+TGG + chr1.2:57594016-57594035 MS.gene67187:intron 25.0%
AAATTATATATGGTCTAGGG+CGG + chr1.2:57593502-57593521 MS.gene67187:intron 30.0%
AACTCTACCTTAATGAAGAA+AGG - chr1.2:57594370-57594389 None:intergenic 30.0%
AATAGGTTACTTGAAGTAAG+AGG + chr1.2:57592985-57593004 MS.gene67187:CDS 30.0%
AGAAACAATATGTCTTCTTC+TGG + chr1.2:57594608-57594627 MS.gene67187:CDS 30.0%
AGTTCGAATATGTAATCTCA+AGG - chr1.2:57595489-57595508 None:intergenic 30.0%
CATCATAAAATCCGTAGATA+CGG - chr1.2:57594701-57594720 None:intergenic 30.0%
CTTAATCAAGATTGATCTTG+TGG + chr1.2:57594247-57594266 MS.gene67187:intron 30.0%
CTTTATAGTTTAGCAAATCG+CGG - chr1.2:57593996-57594015 None:intergenic 30.0%
GAAAAGATTATCTGTATGCA+TGG + chr1.2:57594804-57594823 MS.gene67187:CDS 30.0%
GTATATATCAGTTGTTCATG+AGG - chr1.2:57595559-57595578 None:intergenic 30.0%
GTTCGAATATGTAATCTCAA+GGG - chr1.2:57595488-57595507 None:intergenic 30.0%
TAGTAGTCCAACAAAATTCA+AGG + chr1.2:57595350-57595369 MS.gene67187:CDS 30.0%
TCAAATTCTCCACAGTAATT+AGG - chr1.2:57595216-57595235 None:intergenic 30.0%
TTGCATATATCAGACCTTTA+TGG + chr1.2:57594131-57594150 MS.gene67187:intron 30.0%
TTTCATTTCTCTATGTTACC+AGG + chr1.2:57594471-57594490 MS.gene67187:intron 30.0%
! AAAAAGCCTAAGTTTAGCTT+TGG + chr1.2:57595306-57595325 MS.gene67187:CDS 30.0%
! AACTTAGGCTTTTTATCAAG+AGG - chr1.2:57595300-57595319 None:intergenic 30.0%
!! ATATACATTTGGTGCTGATA+GGG + chr1.2:57594977-57594996 MS.gene67187:CDS 30.0%
!! GTTTTAGATTATAGGAAGTG+GGG + chr1.2:57594018-57594037 MS.gene67187:intron 30.0%
AAATCCGTAGATACGGTTTA+TGG - chr1.2:57594694-57594713 None:intergenic 35.0%
AAGATTATCTGTATGCATGG+TGG + chr1.2:57594807-57594826 MS.gene67187:CDS 35.0%
AATAAATAATTCGAGCGAGG+AGG - chr1.2:57594552-57594571 None:intergenic 35.0%
ACATCACTTGATTGAGCTAA+CGG - chr1.2:57593920-57593939 None:intergenic 35.0%
ATGCTTAAACAATGCCCTTA+GGG - chr1.2:57593566-57593585 None:intergenic 35.0%
ATGTTACCAGGAGATATTCA+TGG + chr1.2:57594483-57594502 MS.gene67187:intron 35.0%
CTACCTTAATGAAGAAAGGT+AGG - chr1.2:57594366-57594385 None:intergenic 35.0%
GAATATGTAATCTCAAGGGA+CGG - chr1.2:57595484-57595503 None:intergenic 35.0%
GGAGATACAATGTGAAACTA+TGG + chr1.2:57594730-57594749 MS.gene67187:CDS 35.0%
GTCTATTATGTGACCTCTTA+TGG + chr1.2:57594901-57594920 MS.gene67187:CDS 35.0%
TAAGAGGTCACATAATAGAC+CGG - chr1.2:57594901-57594920 None:intergenic 35.0%
TAGAATTGTGGAGTCCATAA+AGG - chr1.2:57594148-57594167 None:intergenic 35.0%
TATTGACCATGAATATCTCC+TGG - chr1.2:57594492-57594511 None:intergenic 35.0%
TCACCTACCTTTCTTCATTA+AGG + chr1.2:57594360-57594379 MS.gene67187:intron 35.0%
TGTGGAGAATTTGACAATTC+TGG + chr1.2:57595222-57595241 MS.gene67187:CDS 35.0%
TTTCTTTATGTTGCAGGTTG+TGG + chr1.2:57595137-57595156 MS.gene67187:intron 35.0%
! AAGTCTGAAAGCTCTGTTTT+TGG + chr1.2:57594098-57594117 MS.gene67187:intron 35.0%
! CAAAAAATGCTTGCTGAAAG+TGG - chr1.2:57595628-57595647 None:intergenic 35.0%
! TTTTCAGCACCTAATTACTG+TGG + chr1.2:57595204-57595223 MS.gene67187:CDS 35.0%
!! AGTCTGAAAGCTCTGTTTTT+GGG + chr1.2:57594099-57594118 MS.gene67187:intron 35.0%
!! CATATACATTTGGTGCTGAT+AGG + chr1.2:57594976-57594995 MS.gene67187:CDS 35.0%
!! TAAAGGTGTTACAGAGGTAA+AGG - chr1.2:57593895-57593914 None:intergenic 35.0%
!! TGATTGAGCTAACGGATTAA+AGG - chr1.2:57593912-57593931 None:intergenic 35.0%
!!! ATTTTAAAACTTTCCCGCGT+AGG + chr1.2:57595449-57595468 MS.gene67187:intron 35.0%
!!! CACTTTCAGCAAGCATTTTT+TGG + chr1.2:57595626-57595645 MS.gene67187:intron 35.0%
!!! TACGCGGGAAAGTTTTAAAA+TGG - chr1.2:57595450-57595469 None:intergenic 35.0%
AGAATAGGCCACAATAGTGT+TGG - chr1.2:57594442-57594461 None:intergenic 40.0%
AGCTCAATCAAGTGATGTCA+TGG + chr1.2:57593922-57593941 MS.gene67187:intron 40.0%
CGCGGAGTTTCATATACATT+TGG + chr1.2:57594966-57594985 MS.gene67187:CDS 40.0%
CTAGTTTCAACAGATTAGGC+TGG - chr1.2:57593089-57593108 None:intergenic 40.0%
CTTGCTAACAAGTGTCCTAA+GGG - chr1.2:57593696-57593715 None:intergenic 40.0%
GAATAGGCCACAATAGTGTT+GGG - chr1.2:57594441-57594460 None:intergenic 40.0%
GATGCTTAAACAATGCCCTT+AGG - chr1.2:57593567-57593586 None:intergenic 40.0%
GCCTAATCTGTTGAAACTAG+AGG + chr1.2:57593089-57593108 MS.gene67187:CDS 40.0%
GCTTCCATAAACCGTATCTA+CGG + chr1.2:57594687-57594706 MS.gene67187:CDS 40.0%
GTGCTTCCAAAGCTAAACTT+AGG - chr1.2:57595315-57595334 None:intergenic 40.0%
GTTCCTTAAACAATGCCCTT+AGG + chr1.2:57593678-57593697 MS.gene67187:intron 40.0%
TACTAGGATCAGACCATAAG+AGG - chr1.2:57594917-57594936 None:intergenic 40.0%
TAGTAGTGATGTTCGTGGTT+GGG + chr1.2:57594932-57594951 MS.gene67187:CDS 40.0%
TCACATAATAGACCGGATTC+AGG - chr1.2:57594894-57594913 None:intergenic 40.0%
TCTTGCTAACAAGTGTCCTA+AGG - chr1.2:57593697-57593716 None:intergenic 40.0%
TGAATACAGAACTTGCACTG+AGG + chr1.2:57595386-57595405 MS.gene67187:intron 40.0%
TGTTATGGAAGTAGCGAGTA+GGG + chr1.2:57594060-57594079 MS.gene67187:intron 40.0%
TTAGGAGACTATGTTGATCG+CGG + chr1.2:57594573-57594592 MS.gene67187:CDS 40.0%
TTGAAGTAAGAGGAAGACCA+GGG + chr1.2:57592995-57593014 MS.gene67187:CDS 40.0%
TTGTTATGGAAGTAGCGAGT+AGG + chr1.2:57594059-57594078 MS.gene67187:intron 40.0%
TTGTTTCAACTGTTTGCCTG+TGG + chr1.2:57594767-57594786 MS.gene67187:CDS 40.0%
! GCCTCTAGTTTCAACAGATT+AGG - chr1.2:57593093-57593112 None:intergenic 40.0%
! TGCTTTTCCCAACACTATTG+TGG + chr1.2:57594431-57594450 MS.gene67187:intron 40.0%
!! ACGGATTAAAGGTGTTACAG+AGG - chr1.2:57593901-57593920 None:intergenic 40.0%
!! TGTCCTAAGGGCATTGTTTA+AGG - chr1.2:57593684-57593703 None:intergenic 40.0%
!! TTTGATTTGTAGGGCTCATC+AGG + chr1.2:57595031-57595050 MS.gene67187:CDS 40.0%
!!! TGAAAGCTCTGTTTTTGGGT+TGG + chr1.2:57594103-57594122 MS.gene67187:intron 40.0%
ACGTCGTCAATCAAGTGCAA+AGG + chr1.2:57593223-57593242 MS.gene67187:intron 45.0%
AGAACTTGCACTGAGGTAGA+GGG + chr1.2:57595393-57595412 MS.gene67187:intron 45.0%
AGTAGTGATGTTCGTGGTTG+GGG + chr1.2:57594933-57594952 MS.gene67187:CDS 45.0%
ATGGAACGTGGAGTTGTTGA+TGG + chr1.2:57592955-57592974 MS.gene67187:CDS 45.0%
ATGTTGCAGGTTGTGGAAGA+CGG + chr1.2:57595144-57595163 MS.gene67187:intron 45.0%
ATTCGTGGTTACCCCTTAGA+AGG - chr1.2:57594665-57594684 None:intergenic 45.0%
CAACCACGAACATCACTACT+AGG - chr1.2:57594933-57594952 None:intergenic 45.0%
CTAGTAGTGATGTTCGTGGT+TGG + chr1.2:57594931-57594950 MS.gene67187:CDS 45.0%
CTTGAAGTAAGAGGAAGACC+AGG + chr1.2:57592994-57593013 MS.gene67187:CDS 45.0%
GATCCTAGTAGTGATGTTCG+TGG + chr1.2:57594927-57594946 MS.gene67187:CDS 45.0%
GTAGCGAGTAGGGATCAAAT+TGG + chr1.2:57594070-57594089 MS.gene67187:intron 45.0%
GTCAATATTCCGACCTTCTG+AGG + chr1.2:57594505-57594524 MS.gene67187:CDS 45.0%
TCTTGCGAACATAGCTGATG+TGG + chr1.2:57593768-57593787 MS.gene67187:intron 45.0%
TTTCAACTGTTTGCCTGTGG+CGG + chr1.2:57594770-57594789 MS.gene67187:CDS 45.0%
! GAGGCACCTATTAAGATCTG+TGG + chr1.2:57593108-57593127 MS.gene67187:CDS 45.0%
! GGTTTATGGAAGCACATTCG+TGG - chr1.2:57594680-57594699 None:intergenic 45.0%
!!! AGCTCTGTTTTTGGGTTGGT+AGG + chr1.2:57594107-57594126 MS.gene67187:intron 45.0%
!!! CTTCTGAGGCTTTTCGAGTA+CGG + chr1.2:57594519-57594538 MS.gene67187:CDS 45.0%
CAGAACTTGCACTGAGGTAG+AGG + chr1.2:57595392-57595411 MS.gene67187:intron 50.0%
CCGGATTCAGGAACTTCACA+CGG - chr1.2:57594882-57594901 None:intergenic 50.0%
CCGTGTGAAGTTCCTGAATC+CGG + chr1.2:57594879-57594898 MS.gene67187:CDS 50.0%
CGTACTCGAAAAGCCTCAGA+AGG - chr1.2:57594521-57594540 None:intergenic 50.0%
CTCGAAAAGCCTCAGAAGGT+CGG - chr1.2:57594517-57594536 None:intergenic 50.0%
CTTGCGAACAAGTGCCCTAA+GGG + chr1.2:57593549-57593568 MS.gene67187:intron 50.0%
TCTTGCGAACAAGTGCCCTA+AGG + chr1.2:57593548-57593567 MS.gene67187:intron 50.0%
AAGAGGAAGACCAGGGAAGC+AGG + chr1.2:57593002-57593021 MS.gene67187:CDS 55.0%
ACGGAGTACAAAGCCTACGC+GGG - chr1.2:57595465-57595484 None:intergenic 55.0%
GAAAGCTGAACCTGCTTCCC+TGG - chr1.2:57593015-57593034 None:intergenic 55.0%
GACGGAGTACAAAGCCTACG+CGG - chr1.2:57595466-57595485 None:intergenic 55.0%
TCATCAATGAGAGCCGCCAC+AGG - chr1.2:57594786-57594805 None:intergenic 55.0%
!!! CTGAGGCTTTTCGAGTACGG+TGG + chr1.2:57594522-57594541 MS.gene67187:CDS 55.0%
GGTTGGGGAGAAAGTGAGCG+CGG + chr1.2:57594948-57594967 MS.gene67187:CDS 60.0%
! GGTCTAGGGCGGTTGCAGTT+AGG + chr1.2:57593513-57593532 MS.gene67187:intron 60.0%
GGGCGGTTGCAGTTAGGCTG+CGG + chr1.2:57593519-57593538 MS.gene67187:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 57592955 57595661 57592955 ID=MS.gene67187
chr1.2 mRNA 57592955 57595661 57592955 ID=MS.gene67187.t1;Parent=MS.gene67187
chr1.2 exon 57592955 57593129 57592955 ID=MS.gene67187.t1.exon1;Parent=MS.gene67187.t1
chr1.2 CDS 57592955 57593129 57592955 ID=cds.MS.gene67187.t1;Parent=MS.gene67187.t1
chr1.2 exon 57594493 57595052 57594493 ID=MS.gene67187.t1.exon2;Parent=MS.gene67187.t1
chr1.2 CDS 57594493 57595052 57594493 ID=cds.MS.gene67187.t1;Parent=MS.gene67187.t1
chr1.2 exon 57595153 57595371 57595153 ID=MS.gene67187.t1.exon3;Parent=MS.gene67187.t1
chr1.2 CDS 57595153 57595371 57595153 ID=cds.MS.gene67187.t1;Parent=MS.gene67187.t1
chr1.2 exon 57595635 57595661 57595635 ID=MS.gene67187.t1.exon4;Parent=MS.gene67187.t1
chr1.2 CDS 57595635 57595661 57595635 ID=cds.MS.gene67187.t1;Parent=MS.gene67187.t1
Gene Sequence

>MS.gene67187

ATGGAACGTGGAGTTGTTGATGGTATTATCAATAGGTTACTTGAAGTAAGAGGAAGACCAGGGAAGCAGGTTCAGCTTTCTGAAGCAGAAATCAAACAGCTTTGTCTTGTCTCTAAAGATATCTTCATGAACCAGCCTAATCTGTTGAAACTAGAGGCACCTATTAAGATCTGTGGTATATTTTCTTCATCAAACTTATGTTTCTACTTTTTTGTTAATTGATTTAATTAGTATAGGATTTAATTTGTATACATCGGTAAAGGTTTTTACGTCGTCAATCAAGTGCAAAGGTTTTTACATCGTCAATCAATTATAATTTATTAGATGATTGAAAACATTAGTCTCTGTTTAACTATTACAAATTTTATAGTGACACAGATGAATGATTGTGATTTGTTGACCGTATAAAACTTGTTACGATGATTTGTGTATGAAAATTAATCTCATTATTAAACTATGTGTGGGTTATTGTGCCTAACATTAAAACAAGTTAAGACTATAGCATAAGTCTTTTCATACAAAGTAATTATATATGGTCTTGATAAAAAAATTATATATGGTCTAGGGCGGTTGCAGTTAGGCTGCGGCGAAATTCTTGCGAACAAGTGCCCTAAGGGCATTGTTTAAGCATCTTAAAGTAGCAATTTTTGCATTGGAAATTGTACGGTTAAGACTTTAAAAAGTGTTTGACTTGTATTTTCAAGACATAGTTTCTTTTTTTGGGTTCCTTAAACAATGCCCTTAGGACACTTGTTAGCAAGACCCAAAAATTATATATGGTCTAGGGCGGTTGCAGTTAGGCTGCGGCGAAATTCTTGCGAACATAGCTGATGTGGACACATTTGCGATTGTGTTGTCATTTTTATTTTGAATGAATAAATGTTAGTATGTTAATTGTTCTGTCAGTATTTTCTATATTTGTCGGGATTCGAAGTCACCTTTACCTCTGTAACACCTTTAATCCGTTAGCTCAATCAAGTGATGTCATGGCATAGACTTTAAAAACTTTTATATCGCGCTCACAATTGCAGTTGCAAACCGCGATTTGCTAAACTATAAAGTTGTTTTAGATTATAGGAAGTGGGGAAGTTATTGTTGTTGTTGTTGTTATGGAAGTAGCGAGTAGGGATCAAATTGGTAAACAAGTCTGAAAGCTCTGTTTTTGGGTTGGTAGGATTGCATATATCAGACCTTTATGGACTCCACAATTCTAATTAAACTGATGTGGTCTCAATAACTGATAATAACCTAATTATTCATTCTTGTTTCAATTTTCTATCTGTGTATTACTTCTTAATCAAGATTGATCTTGTGGATGAGCTCTCATTAAGTTTAATTAAGATTAATTTTGAGGATGTTATGTTAGAACAAAGTGTTTGTCTCATGTGCCAACATATTTGATATTTCACCTACCTTTCTTCATTAAGGTAGAGTTTTATGCTTTCCAAATTTTCTAGTATCATATTTCATGATAGATGCTTTTCCCAACACTATTGTGGCCTATTCTAATGTCTTGTTTCATTTCTCTATGTTACCAGGAGATATTCATGGTCAATATTCCGACCTTCTGAGGCTTTTCGAGTACGGTGGATTTCCTCCTCGCTCGAATTATTTATTTTTAGGAGACTATGTTGATCGCGGAAAACAAAGTCTAGAAACAATATGTCTTCTTCTGGCATATAAAATAAAATATCCTAAAAACTTTTTCCTTCTAAGGGGTAACCACGAATGTGCTTCCATAAACCGTATCTACGGATTTTATGATGAATGTAAAAGGAGATACAATGTGAAACTATGGAAAATGTTTACAGATTGTTTCAACTGTTTGCCTGTGGCGGCTCTCATTGATGAAAAGATTATCTGTATGCATGGTGGACTCTCTCCTGAATTACACGATTTAAATCAGATAAAGAATTTGCGTCGTCCGTGTGAAGTTCCTGAATCCGGTCTATTATGTGACCTCTTATGGTCTGATCCTAGTAGTGATGTTCGTGGTTGGGGAGAAAGTGAGCGCGGAGTTTCATATACATTTGGTGCTGATAGGGTTAAAGAGTTTCTTCAGAAGCATGATCTTGATTTGATTTGTAGGGCTCATCAGGTAACATATTCATTTCAAATATCGAATTGTAACATAATAAGAGTCCAAATTCTAAGTTTATACATACTAAGGTGATTGTGTAATTTTCTTTATGTTGCAGGTTGTGGAAGACGGATACGAGTTCTTTGCGAATAGACAACTTGTAACAATCTTTTCAGCACCTAATTACTGTGGAGAATTTGACAATTCTGGTGCTATGATGACTGTAAATGAATCACTTGTGTGTTCTTTTCAAATACTGAAGCCTCTTGATAAAAAGCCTAAGTTTAGCTTTGGAAGCACAACTACAGTCAAGTCTAGTAGTCCAACAAAATTCAAGGTATTTTGATTTCTTGAATACAGAACTTGCACTGAGGTAGAGGGAAATTATTATGAATTAAAATAGAGCTTATAGCCATTTTAAAACTTTCCCGCGTAGGCTTTGTACTCCGTCCCTTGAGATTACATATTCGAACTCTCTTTTTTTTGAAAGACATATTCAAACTCTTAACACTGAGCTGACACCTCATGAACAACTGATATATACGCACTTATCATCTCTTTCAAATTTTACAAGATTTGTTTTGTTTCTTCCACTTTCAGCAAGCATTTTTTGGTGCTAAAGAATGA
Protein sequence

>MS.gene67187.t1

MERGVVDGIINRLLEVRGRPGKQVQLSEAEIKQLCLVSKDIFMNQPNLLKLEAPIKICGDIHGQYSDLLRLFEYGGFPPRSNYLFLGDYVDRGKQSLETICLLLAYKIKYPKNFFLLRGNHECASINRIYGFYDECKRRYNVKLWKMFTDCFNCLPVAALIDEKIICMHGGLSPELHDLNQIKNLRRPCEVPESGLLCDLLWSDPSSDVRGWGESERGVSYTFGADRVKEFLQKHDLDLICRAHQVVEDGYEFFANRQLVTIFSAPNYCGEFDNSGAMMTVNESLVCSFQILKPLDKKPKFSFGSTTTVKSSSPTKFKQAFFGAKE