AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67732


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 0 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAATTACATAAACATGT+TGG - 14593:189-208 MS.gene67732:CDS 20.0%
!!! ACTTTTTGTGCTGTTTGTGT+TGG - 14593:215-234 MS.gene67732:CDS 35.0%
ACCACCTCCAATGCTTTCTA+GGG + 14593:283-302 MS.gene67732:intergenic 45.0%
! GAAGAGTCCCTAGAAAGCAT+TGG - 14593:273-292 MS.gene67732:CDS 45.0%
!! GTGCTGTTTGTGTTGGTGAT+AGG - 14593:222-241 MS.gene67732:CDS 45.0%
AAAGGTGGAGGATTTGGAGG+TGG - 14593:990-1009 MS.gene67732:CDS 50.0%
ATGAAGGAGGAGAAGGTGGT+GGG - 14593:559-578 MS.gene67732:CDS 50.0%
CACCACCTCCAATGCTTTCT+AGG + 14593:284-303 MS.gene67732:intergenic 50.0%
GAAAAAGGCGGAGGATTTGG+AGG - 14593:495-514 MS.gene67732:CDS 50.0%
GGAAAAGGTGGAGGATTTGG+AGG - 14593:987-1006 MS.gene67732:CDS 50.0%
GGAGGATTTGGAGGTGGTAT+TGG - 14593:606-625 MS.gene67732:CDS 50.0%
GGTGGAAAAGGTGGAGGATT+TGG - 14593:984-1003 MS.gene67732:CDS 50.0%
GTGGAAAAAGGCGGAGGATT+TGG - 14593:492-511 MS.gene67732:CDS 50.0%
TGAAAAGGCGGAGGATTTGG+AGG - 14593:885-904 MS.gene67732:CDS 50.0%
TTTCGAAAAGGAGGCGGACA+TGG - 14593:324-343 MS.gene67732:CDS 50.0%
! GGAGGTGGATTTCGAAAAGG+AGG - 14593:315-334 MS.gene67732:CDS 50.0%
! GGATTTGGAGGTGGTATTGG+AGG - 14593:609-628 MS.gene67732:intron 50.0%
! GGTGGAGGTGGATTTCGAAA+AGG - 14593:312-331 MS.gene67732:CDS 50.0%
! GGTGGATTTCGAAAAGGAGG+CGG - 14593:318-337 MS.gene67732:CDS 50.0%
! TTTGGAGGTGGTATTGGAGG+TGG - 14593:612-631 MS.gene67732:intron 50.0%
!! AGCATTGGAGGTGGTGGTTT+TGG - 14593:288-307 MS.gene67732:CDS 50.0%
!! CTAGAAAGCATTGGAGGTGG+TGG - 14593:282-301 MS.gene67732:CDS 50.0%
!! GAGTCCCTAGAAAGCATTGG+AGG - 14593:276-295 MS.gene67732:CDS 50.0%
!! TCCCTAGAAAGCATTGGAGG+TGG - 14593:279-298 MS.gene67732:CDS 50.0%
!! TTGGAGGTGGTGGTGGAAAA+GGG - 14593:379-398 MS.gene67732:CDS 50.0%
!! TTTGGAGGTGGTGGTGGAAA+AGG - 14593:378-397 MS.gene67732:CDS 50.0%
!!! ATTGGAGGTGGTGGTTTTGG+TGG - 14593:291-310 MS.gene67732:CDS 50.0%
AAAGGCGGAGGATTTGGAGG+TGG - 14593:396-415 MS.gene67732:CDS 55.0%
ATGGAGGAGGAGAAGGTGGT+GGG - 14593:457-476 MS.gene67732:CDS 55.0%
CACCACCTTCTCCTCCTTCA+TGG + 14593:560-579 MS.gene67732:intergenic 55.0%
CATGAAGGAGGAGAAGGTGG+TGG - 14593:558-577 MS.gene67732:CDS 55.0%
CATGGAGGAGAGAAGGTGGT+GGG - 14593:648-667 MS.gene67732:intron 55.0%
CGGTGAAAAGGCGGAGGATT+TGG - 14593:882-901 MS.gene67732:CDS 55.0%
GAAAAGGGCGGAGGATTTGG+AGG - 14593:1090-1109 MS.gene67732:CDS 55.0%
GATTGGAGGTGGCGTGGAAA+AGG - 14593:670-689 MS.gene67732:intron 55.0%
GGAAAAGGCGGAGGATTTGG+AGG - 14593:393-412 MS.gene67732:CDS 55.0%
GGAGGATTTGGAGGTGGTAC+AGG - 14593:402-421 MS.gene67732:CDS 55.0%
GGAGGATTTGGAGGTGGTAC+TGG - 14593:402-421 MS.gene67732:CDS 55.0%
GGAGGATTTGGAGGTGGTAG+TGG - 14593:504-523 MS.gene67732:CDS 55.0%
GGATTTGGAGGTGGTACAGG+TGG - 14593:405-424 MS.gene67732:CDS 55.0%
GGTGGAAAAGGCGGAGGATT+TGG - 14593:390-409 MS.gene67732:CDS 55.0%
GTGGAAAAGGGCGGAGGATT+TGG - 14593:1087-1106 MS.gene67732:CDS 55.0%
TGAAGGAGGAGAAGGTGGTG+GGG - 14593:560-579 MS.gene67732:CDS 55.0%
TTTGGAGGTGGTACAGGTGG+TGG - 14593:408-427 MS.gene67732:CDS 55.0%
! AAAGGCGGTGGATTTGGAGG+TGG - 14593:688-707 MS.gene67732:intron 55.0%
! ACAGGTGGTGGTATTGGAGG+TGG - 14593:420-439 MS.gene67732:intron 55.0%
! ATAGGAGGAGGTGTAGGTGG+CGG - 14593:354-373 MS.gene67732:CDS 55.0%
! CGTGGAAAAGGCGGTGGATT+TGG - 14593:682-701 MS.gene67732:intron 55.0%
! GGAAAAGGCGGTGGATTTGG+AGG - 14593:685-704 MS.gene67732:intron 55.0%
! GGATTTGGAGGTGGTACTGG+TGG - 14593:799-818 MS.gene67732:CDS 55.0%
! GGTACAGGTGGTGGTATTGG+AGG - 14593:417-436 MS.gene67732:intron 55.0%
! GGTATAGGAGGAGGTGTAGG+TGG - 14593:351-370 MS.gene67732:CDS 55.0%
! GGTATTGGAGGTGGTGTTGG+TGG - 14593:429-448 MS.gene67732:intron 55.0%
! GGTGGATTTGGAGGTGGTAG+TGG - 14593:694-713 MS.gene67732:intron 55.0%
! GGTGGTACAGGTGGTGGTAT+TGG - 14593:414-433 MS.gene67732:intron 55.0%
! GGTGGTATAGGAGGAGGTGT+AGG - 14593:348-367 MS.gene67732:CDS 55.0%
! GGTGGTATTGGAGGTGGTGT+TGG - 14593:426-445 MS.gene67732:intron 55.0%
! TTGGAGGTGGCGGTGGAAAA+AGG - 14593:480-499 MS.gene67732:CDS 55.0%
!! ACTGGTGGTGGTATTGGAGG+TGG - 14593:814-833 MS.gene67732:CDS 55.0%
!! AGAAGGTGGTGGATTGGAGG+TGG - 14593:467-486 MS.gene67732:CDS 55.0%
!! AGAGAAGGTGGTGGGATTGG+AGG - 14593:656-675 MS.gene67732:intron 55.0%
!! AGGAGAAGGTGGTGGATTGG+AGG - 14593:464-483 MS.gene67732:CDS 55.0%
!! AGGAGAGAAGGTGGTGGGAT+TGG - 14593:653-672 MS.gene67732:intron 55.0%
!! AGGAGGAGAAGGTGGTGGAT+TGG - 14593:461-480 MS.gene67732:CDS 55.0%
!! AGTGGTGGTGGTATTGGAGG+TGG - 14593:522-541 MS.gene67732:CDS 55.0%
!! ATTGGAGGTGGTGTTGGTGG+AGG - 14593:432-451 MS.gene67732:intron 55.0%
!! GATTTGGAGGTGGTGCTGGT+GGG - 14593:1103-1122 MS.gene67732:CDS 55.0%
!! GGATTTGGAGGTGGTAGTGG+TGG - 14593:507-526 MS.gene67732:CDS 55.0%
!! GGTACTGGTGGTGGTATTGG+AGG - 14593:811-830 MS.gene67732:CDS 55.0%
!! GGTAGTGGTGGTGGTATTGG+AGG - 14593:519-538 MS.gene67732:CDS 55.0%
!! GGTGGTACTGGTGGTGGTAT+TGG - 14593:808-827 MS.gene67732:CDS 55.0%
!! GGTGGTAGTGGTGGTGGTAT+TGG - 14593:516-535 MS.gene67732:CDS 55.0%
!! TTTGGAGGTGGTACTGGTGG+TGG - 14593:802-821 MS.gene67732:CDS 55.0%
!! TTTGGAGGTGGTAGTGGTGG+TGG - 14593:510-529 MS.gene67732:CDS 55.0%
AAGGGCGGAGGATTTGGAGG+TGG - 14593:1093-1112 MS.gene67732:CDS 60.0%
AGGCCATGGAGGAGAGAAGG+TGG - 14593:644-663 MS.gene67732:intron 60.0%
ATGGAGGAGGAGAAGGCGGT+GGG - 14593:1051-1070 MS.gene67732:CDS 60.0%
CACCACCTTCTCCTCCTCCA+TGG + 14593:458-477 MS.gene67732:intergenic 60.0%
CATGGAGGAGGAGAAGGTGG+TGG - 14593:456-475 MS.gene67732:intron 60.0%
CATGGCGGTGGTATAGGAGG+AGG - 14593:342-361 MS.gene67732:CDS 60.0%
CCACCACCTTCTCTCCTCCA+TGG + 14593:650-669 MS.gene67732:intergenic 60.0%
CCATGGAGGAGAAGGTGGTG+GGG - 14593:849-868 MS.gene67732:CDS 60.0%
CCATGGAGGAGAGAAGGTGG+TGG - 14593:647-666 MS.gene67732:intron 60.0%
CGAAAAGGAGGCGGACATGG+CGG - 14593:327-346 MS.gene67732:CDS 60.0%
GCCATGGAGGAGAAGGTGGT+GGG - 14593:848-867 MS.gene67732:CDS 60.0%
GGACATGGCGGTGGTATAGG+AGG - 14593:339-358 MS.gene67732:CDS 60.0%
GGAGGATTTGGAGGTGGTGC+TGG - 14593:1099-1118 MS.gene67732:CDS 60.0%
GGAGGCCATGAAGGAGGAGA+AGG - 14593:552-571 MS.gene67732:CDS 60.0%
GGCCATGAAGGAGGAGAAGG+TGG - 14593:555-574 MS.gene67732:CDS 60.0%
TGGAGGAGGAGAAGGTGGTG+GGG - 14593:458-477 MS.gene67732:CDS 60.0%
TGGAGGCCATGGAGGAGAGA+AGG - 14593:641-660 MS.gene67732:intron 60.0%
TGGAGGTGGCGTGGAAAAGG+CGG - 14593:673-692 MS.gene67732:intron 60.0%
! GAGGTGGCGGTGGAAAAAGG+CGG - 14593:483-502 MS.gene67732:CDS 60.0%
! GGATTTGGAGGTGGTGCTGG+TGG - 14593:1102-1121 MS.gene67732:CDS 60.0%
! GGTATTGGAGGTGGTGCTGG+TGG - 14593:531-550 MS.gene67732:CDS 60.0%
! GGTGGTATTGGAGGTGGTGC+CGG - 14593:528-547 MS.gene67732:CDS 60.0%
! GGTGGTATTGGAGGTGGTGC+TGG - 14593:528-547 MS.gene67732:CDS 60.0%
! GTTGGAGGTGGCGGTGGAAA+AGG - 14593:582-601 MS.gene67732:CDS 60.0%
! TAGGAGGAGGTGTAGGTGGC+GGG - 14593:355-374 MS.gene67732:CDS 60.0%
! TGGAGGTGGCGGTGAAAAGG+CGG - 14593:873-892 MS.gene67732:CDS 60.0%
!! AGGTGGTGGATTGGAGGTGG+CGG - 14593:470-489 MS.gene67732:CDS 60.0%
!! ATTGGAGGTGGTGCTGGTGG+AGG - 14593:534-553 MS.gene67732:CDS 60.0%
!! GAAGGTGGTGGGATTGGAGG+TGG - 14593:659-678 MS.gene67732:intron 60.0%
!! GAGGTGGTGGTGGAAAAGGG+CGG - 14593:1078-1097 MS.gene67732:CDS 60.0%
!! GGAGGTGGTGGTGGAAAAGG+CGG - 14593:381-400 MS.gene67732:CDS 60.0%
!!! GGAGGTGGTGGTTTTGGTGG+TGG - 14593:294-313 MS.gene67732:CDS 60.0%
!!! GGTGGTGGTTTTGGTGGTGG+AGG - 14593:297-316 MS.gene67732:CDS 60.0%
!!! GGTGGTTTTGGTGGTGGAGG+TGG - 14593:300-319 MS.gene67732:CDS 60.0%
AAAGGAGGCGGACATGGCGG+TGG - 14593:330-349 MS.gene67732:CDS 65.0%
AGGTGGCGTGGAAAAGGCGG+TGG - 14593:676-695 MS.gene67732:intron 65.0%
CACCGCCTTCTCCTCCTCCA+TGG + 14593:1052-1071 MS.gene67732:intergenic 65.0%
CATGGAGGAGGAGAAGGCGG+TGG - 14593:1050-1069 MS.gene67732:CDS 65.0%
CCCCACCACCTTCTCCTCCA+TGG + 14593:852-871 MS.gene67732:intergenic 65.0%
GCTGGTGGAGGCCATGAAGG+AGG - 14593:546-565 MS.gene67732:CDS 65.0%
GGAGGCCATGGAGGAGAAGG+TGG - 14593:844-863 MS.gene67732:CDS 65.0%
GGAGGCCATGGAGGAGGAGA+AGG - 14593:450-469 MS.gene67732:intron 65.0%
GGCCATGGAGGAGAAGGTGG+TGG - 14593:847-866 MS.gene67732:CDS 65.0%
GGCCATGGAGGAGGAGAAGG+CGG - 14593:453-472 MS.gene67732:intron 65.0%
GGCCATGGAGGAGGAGAAGG+TGG - 14593:453-472 MS.gene67732:intron 65.0%
GGCGGACATGGCGGTGGTAT+AGG - 14593:336-355 MS.gene67732:CDS 65.0%
GGTGGAGGCCATGGAGGAGA+AGG - 14593:639-658 MS.gene67732:intron 65.0%
GTGGCGGTGGAAAAAGGCGG+AGG - 14593:486-505 MS.gene67732:CDS 65.0%
TCCTCCTCCATGGCCTCCAC+CGG + 14593:448-467 MS.gene67732:intergenic 65.0%
TGGTGGGATTGGAGGTGGCG+TGG - 14593:664-683 MS.gene67732:intron 65.0%
! AGGTGGCGGTGAAAAGGCGG+AGG - 14593:876-895 MS.gene67732:CDS 65.0%
! GAAGGCGGTGGGTTTGGAGG+TGG - 14593:1062-1081 MS.gene67732:CDS 65.0%
! GGAGAAGGCGGTGGGTTTGG+AGG - 14593:1059-1078 MS.gene67732:CDS 65.0%
! GGAGGAGAAGGCGGTGGGTT+TGG - 14593:1056-1075 MS.gene67732:CDS 65.0%
! GGAGGTGGCGGTGGAAAAGG+CGG - 14593:585-604 MS.gene67732:CDS 65.0%
! GGAGGTGGCGGTGGAAAAGG+TGG - 14593:585-604 MS.gene67732:CDS 65.0%
! GGCTGGAGGTGGCGGTGAAA+AGG - 14593:870-889 MS.gene67732:CDS 65.0%
! GGTATTGGAGGTGGTGCCGG+TGG - 14593:921-940 MS.gene67732:CDS 65.0%
! GGTGGCGGTGGAAAAGGTGG+AGG - 14593:978-997 MS.gene67732:CDS 65.0%
! GGTGGGTTTGGAGGTGGTGG+TGG - 14593:1068-1087 MS.gene67732:CDS 65.0%
! GTTGGTGGAGGCCATGGAGG+AGG - 14593:444-463 MS.gene67732:intron 65.0%
!! ATTGGAGGTGGTGCCGGTGG+AGG - 14593:924-943 MS.gene67732:CDS 65.0%
!! GAAGGTGGTGGGGTTGGAGG+TGG - 14593:570-589 MS.gene67732:CDS 65.0%
!! GGAGAAGGTGGTGGGGTTGG+AGG - 14593:567-586 MS.gene67732:CDS 65.0%
!! GGAGGAGAAGGTGGTGGGGT+TGG - 14593:564-583 MS.gene67732:CDS 65.0%
!! GGAGGTGTAGGTGGCGGGTT+TGG - 14593:360-379 MS.gene67732:CDS 65.0%
!! GGTGCTGGTGGAGGCCATGA+AGG - 14593:543-562 MS.gene67732:CDS 65.0%
!! GGTGGTGGTGGAAAAGGCGG+AGG - 14593:384-403 MS.gene67732:CDS 65.0%
!! GGTGGTGTTGGTGGAGGCCA+TGG - 14593:438-457 MS.gene67732:intron 65.0%
!! GGTGTAGGTGGCGGGTTTGG+AGG - 14593:363-382 MS.gene67732:CDS 65.0%
!! GGTGTTGGTGGAGGCCATGG+AGG - 14593:441-460 MS.gene67732:intron 65.0%
!! GTAGGTGGCGGGTTTGGAGG+TGG - 14593:366-385 MS.gene67732:CDS 65.0%
!! GTGGTGGTGGAAAAGGGCGG+AGG - 14593:1081-1100 MS.gene67732:CDS 65.0%
!! TGGTGGATTGGAGGTGGCGG+TGG - 14593:473-492 MS.gene67732:CDS 65.0%
!! TTGGAGGTGGTGCTGGTGGG+TGG - 14593:1106-1125 MS.gene67732:CDS 65.0%
GCTGGTGGAGGCCATGGAGG+AGG - 14593:636-655 MS.gene67732:intron 70.0%
! GGCGGGTTTGGAGGTGGTGG+TGG - 14593:372-391 MS.gene67732:CDS 70.0%
! GGCGGTGGGTTTGGAGGTGG+TGG - 14593:1065-1084 MS.gene67732:CDS 70.0%
! GGTGGCGGTGGAAAAGGCGG+AGG - 14593:588-607 MS.gene67732:CDS 70.0%
!! GAAGGTGGTGGGGCTGGAGG+TGG - 14593:859-878 MS.gene67732:CDS 70.0%
!! GGAGAAGGTGGTGGGGCTGG+AGG - 14593:856-875 MS.gene67732:CDS 70.0%
!! GGAGGAGAAGGTGGTGGGGC+TGG - 14593:853-872 MS.gene67732:CDS 70.0%
!! GGTGCTGGTGGAGGCCATGG+AGG - 14593:633-652 MS.gene67732:intron 70.0%
!! GGTGGCGGGTTTGGAGGTGG+TGG - 14593:369-388 MS.gene67732:CDS 70.0%
!! GGTGGTGCTGGTGGAGGCCA+TGG - 14593:540-559 MS.gene67732:CDS 70.0%
!! GGTGGTGGGGTTGGAGGTGG+CGG - 14593:573-592 MS.gene67732:CDS 70.0%
GCCGGTGGAGGCCATGGAGG+AGG - 14593:936-955 MS.gene67732:CDS 75.0%
! GGTGGGGTTGGAGGTGGCGG+TGG - 14593:576-595 MS.gene67732:CDS 75.0%
!! GGTGCCGGTGGAGGCCATGG+AGG - 14593:933-952 MS.gene67732:CDS 75.0%
!! GGTGGTGCCGGTGGAGGCCA+TGG - 14593:930-949 MS.gene67732:CDS 75.0%
!! GGTGGTGGGGCTGGAGGTGG+CGG - 14593:862-881 MS.gene67732:CDS 75.0%


Chromosome Type Strat End Strand Name
14593 gene 186 1133 186 ID=MS.gene67732
14593 mRNA 186 1133 186 ID=MS.gene67732.t1;Parent=MS.gene67732
14593 exon 698 1133 698 ID=MS.gene67732.t1.exon1;Parent=MS.gene67732.t1
14593 CDS 698 1133 698 ID=cds.MS.gene67732.t1;Parent=MS.gene67732.t1
14593 exon 457 627 457 ID=MS.gene67732.t1.exon2;Parent=MS.gene67732.t1
14593 CDS 457 627 457 ID=cds.MS.gene67732.t1;Parent=MS.gene67732.t1
14593 exon 186 427 186 ID=MS.gene67732.t1.exon3;Parent=MS.gene67732.t1
14593 CDS 186 427 186 ID=cds.MS.gene67732.t1;Parent=MS.gene67732.t1
Gene Sequence

>MS.gene67732

ATGACAAAATTACATAAACATGTTGGAATACTTTTTGTGCTGTTTGTGTTGGTGATAGGCATAGTAGAGTGTCGCAAACTCAAAGAAGAAGAGTCCCTAGAAAGCATTGGAGGTGGTGGTTTTGGTGGTGGAGGTGGATTTCGAAAAGGAGGCGGACATGGCGGTGGTATAGGAGGAGGTGTAGGTGGCGGGTTTGGAGGTGGTGGTGGAAAAGGCGGAGGATTTGGAGGTGGTACAGGTGGTGGTATTGGAGGTGGTGTTGGTGGAGGCCATGGAGGAGGAGAAGGTGGTGGATTGGAGGTGGCGGTGGAAAAAGGCGGAGGATTTGGAGGTGGTAGTGGTGGTGGTATTGGAGGTGGTGCTGGTGGAGGCCATGAAGGAGGAGAAGGTGGTGGGGTTGGAGGTGGCGGTGGAAAAGGCGGAGGATTTGGAGGTGGTATTGGAGGTGGTGCTGGTGGAGGCCATGGAGGAGAGAAGGTGGTGGGATTGGAGGTGGCGTGGAAAAGGCGGTGGATTTGGAGGTGGTAGTGGTGGTGGTATTGGAGGTGGTGCTGGTGGAGGCCATGGAGGAGGAGAAGGTGGTGGGGTTGGAGGTGGCGGTGGAAAAGGCGGAGGATTTGGAGGTGGTACTGGTGGTGGTATTGGAGGTGGTGCTGGTGGAGGCCATGGAGGAGAAGGTGGTGGGGCTGGAGGTGGCGGTGAAAAGGCGGAGGATTTGGAGGTGGTACTGGTGGTGGTATTGGAGGTGGTGCCGGTGGAGGCCATGGAGGAGGAGAAGGTGGTGGGGTTGGAGGTGGCGGTGGAAAAGGTGGAGGATTTGGAGGTGGTACTGGTGGTGGTATTGGAGGTGGTGCTGGTGGAGGCCATGGAGGAGGAGAAGGCGGTGGGTTTGGAGGTGGTGGTGGAAAAGGGCGGAGGATTTGGAGGTGGTGCTGGTGGGTGGTAAAG

Protein sequence

>MS.gene67732.t1

MTKLHKHVGILFVLFVLVIGIVECRKLKEEESLESIGGGGFGGGGGFRKGGGHGGGIGGGVGGGFGGGGGKGGGFGGGTGGGIGGGVGGGHGGGEGGGLEVAVEKGGGFGGGSGGGIGGGAGGGHEGGEGGGVGGGGGKGGGFGGGGGFGGGSGGGIGGGAGGGHGGGEGGGVGGGGGKGGGFGGGTGGGIGGGAGGGHGGEGGGFGGGTGGGIGGGAGGGHGGGEGGGVGGGGGKGGGFGGGTGGGIGGGAGGGHGGGEGGGFGGGGGKGRRIWRWCWWVVK