AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68376

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68376.t1 MTR_1g055005 81.679 131 23 1 576 705 47 177 1.75e-52 181
MS.gene68376.t1 MTR_1g055005 85.106 47 7 0 358 404 1 47 4.67e-20 89.4
MS.gene68376.t1 MTR_7g078980 62.264 53 19 1 305 356 59 111 1.71e-14 70.9
MS.gene68376.t1 MTR_3g028350 35.185 108 44 3 376 482 32 114 9.13e-14 69.3
MS.gene68376.t1 MTR_7g078930 62.264 53 19 1 305 356 77 129 1.02e-13 68.9
MS.gene68376.t1 MTR_2g089885 35.922 103 61 3 15 114 2 102 1.35e-12 67.4
MS.gene68376.t1 MTR_6g060670 41.772 79 44 2 36 113 24 101 8.42e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68376.t1 AT5G33406 41.787 347 190 4 330 671 11 350 9.71e-90 291
MS.gene68376.t1 AT4G15020 32.847 548 352 11 102 641 176 715 1.69e-86 290
MS.gene68376.t1 AT4G15020 32.847 548 352 11 102 641 176 715 1.69e-86 290
MS.gene68376.t1 AT3G22220 33.200 500 316 11 148 641 223 710 3.62e-81 275
MS.gene68376.t1 AT3G22220 33.200 500 316 11 148 641 223 710 3.62e-81 275
MS.gene68376.t1 AT3G22220 33.200 500 316 11 148 641 223 710 3.62e-81 275
MS.gene68376.t1 AT3G22220 33.200 500 316 11 148 641 223 710 3.62e-81 275
MS.gene68376.t1 AT3G17450 32.043 465 305 7 147 605 332 791 2.42e-79 273
MS.gene68376.t1 AT3G17450 32.043 465 305 7 147 605 332 791 2.42e-79 273
MS.gene68376.t1 AT1G79740 28.732 623 393 12 36 628 6 607 4.95e-79 267
MS.gene68376.t1 AT1G79740 28.732 623 393 12 36 628 6 607 4.95e-79 267
MS.gene68376.t1 AT1G79740 31.884 483 315 8 147 628 5 474 3.98e-77 258
MS.gene68376.t1 AT1G79740 31.884 483 315 8 147 628 5 474 3.98e-77 258
MS.gene68376.t1 AT3G13030 27.898 509 326 12 126 612 55 544 1.70e-52 191
MS.gene68376.t1 AT3G13030 27.898 509 326 12 126 612 55 544 1.70e-52 191
MS.gene68376.t1 AT3G13030 27.898 509 326 12 126 612 55 544 1.70e-52 191
MS.gene68376.t1 AT3G13030 27.898 509 326 12 126 612 55 544 1.70e-52 191
MS.gene68376.t1 AT3G13020 26.789 601 370 16 52 602 21 601 1.26e-50 187
MS.gene68376.t1 AT5G31412 29.929 421 184 9 37 456 24 334 1.72e-44 166
MS.gene68376.t1 AT3G13030 26.459 514 294 13 126 612 55 511 1.28e-43 166
MS.gene68376.t1 AT1G43260 36.453 203 127 2 148 348 66 268 2.17e-39 148
MS.gene68376.t1 AT3G13010 24.790 476 333 13 135 605 116 571 8.09e-34 137
MS.gene68376.t1 AT1G36095 30.564 337 163 10 55 388 29 297 3.36e-33 130

Find 194 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTAATGCTGGTAGTTTA+TGG 0.143974 1.3:+27418039 MS.gene68376:CDS
TGCTGGTAGTTTATGGTTAT+TGG 0.150578 1.3:+27418046 MS.gene68376:CDS
CAATCTGTTGCTTCCTCTAT+TGG 0.169469 1.3:+27415793 MS.gene68376:CDS
ACATGAGATGTTGCAAAATT+TGG 0.188754 1.3:+27417935 MS.gene68376:CDS
TCAAGAGTGGTCGGACTTAA+AGG 0.234410 1.3:+27417103 MS.gene68376:CDS
TTGTTCTTCAAGTCGCATTT+TGG 0.258584 1.3:+27417162 MS.gene68376:CDS
CTGCCTTCTGCAAAGTTGCT+TGG 0.273006 1.3:-27418187 None:intergenic
CAACGGGGCCAATTATGTTT+TGG 0.301560 1.3:+27416698 MS.gene68376:CDS
AATCTGTTGCTTCCTCTATT+GGG 0.309777 1.3:+27415794 MS.gene68376:CDS
GCTGGTAGTTTATGGTTATT+GGG 0.315171 1.3:+27418047 MS.gene68376:CDS
CTTACCGGCCAAAACATAAT+TGG 0.316047 1.3:-27416706 None:intergenic
AAACTACCAGCATTAATTTC+AGG 0.316356 1.3:-27418035 None:intergenic
TTTCATCTATAACCATTCTT+TGG 0.337391 1.3:+27416950 MS.gene68376:CDS
TAGAAAGCTTTCTTCTTCAA+TGG 0.342613 1.3:+27417496 MS.gene68376:CDS
TTAAATTGGCAGAAGCAATA+AGG 0.345705 1.3:+27417576 MS.gene68376:CDS
GGATAAGTATAGGGCTAGTT+CGG 0.346870 1.3:+27417547 MS.gene68376:CDS
GTAGTAAGTAACTTTAGATT+TGG 0.350250 1.3:-27417739 None:intergenic
ATCCAGAGTTTATTACTGAT+TGG 0.352785 1.3:+27418351 MS.gene68376:CDS
TTGGCTCCTAGTGTTGAATA+AGG 0.358271 1.3:-27418168 None:intergenic
AGTTTGACTGTTAGCTCATC+GGG 0.359053 1.3:+27417772 MS.gene68376:CDS
CATCGGGGTGTGAGAGGAAT+TGG 0.360941 1.3:+27417788 MS.gene68376:CDS
TCCAGAGTTTATTACTGATT+GGG 0.365599 1.3:+27418352 MS.gene68376:CDS
AGTCTACGGGAGGAATTTCC+AGG 0.367684 1.3:+27415914 MS.gene68376:CDS
TGATGACATCCATGCAGATT+TGG 0.370501 1.3:+27417526 MS.gene68376:CDS
TGCAGCTGGTTACCGACAAC+GGG 0.372274 1.3:+27416682 MS.gene68376:CDS
GTTGAAGAAATTGGTCAATA+TGG 0.378019 1.3:+27416360 MS.gene68376:intron
TTCACTCCAAGAAAAGAAAT+AGG 0.380458 1.3:+27417907 MS.gene68376:CDS
TTTCAGCTGCTTGGTGGAAT+AGG 0.383939 1.3:+27417701 MS.gene68376:intron
GTTTATGCTCTTAAAGCTAT+GGG 0.386704 1.3:+27417194 MS.gene68376:CDS
GTTACGACCTTCTTTACTCT+TGG 0.391849 1.3:-27416906 None:intergenic
TAGTTCGGGTAACTTTAAAT+TGG 0.394568 1.3:+27417562 MS.gene68376:CDS
GTTGAGGGAGAAGGCTGAAA+TGG 0.395558 1.3:+27416983 MS.gene68376:CDS
GATGAATTTGTTGGGGAGAT+TGG 0.397591 1.3:+27416645 MS.gene68376:CDS
CACGTGTGATTATTGTTTGT+TGG 0.404102 1.3:+27415891 MS.gene68376:CDS
TGATATATGAAACTCATTGC+AGG 0.408777 1.3:-27417260 None:intergenic
TGTTTATGCTCTTAAAGCTA+TGG 0.411079 1.3:+27417193 MS.gene68376:CDS
TGCAGATTTGGATAAGTATA+GGG 0.412113 1.3:+27417538 MS.gene68376:CDS
AAGGCGGAAGCAGACAAATA+TGG 0.414057 1.3:+27416474 MS.gene68376:CDS
ATGCAGATTTGGATAAGTAT+AGG 0.415922 1.3:+27417537 MS.gene68376:CDS
AGCTGCTTGGTGGAATAGGT+GGG 0.417032 1.3:+27417705 MS.gene68376:intron
GATGGAAGTATAACAGTGTA+AGG 0.417281 1.3:+27415845 MS.gene68376:CDS
GATTTAATGCTAGAAAAGAT+TGG 0.417475 1.3:+27416876 MS.gene68376:CDS
TTGAGATTGTTGACAGGAGG+TGG 0.418360 1.3:+27417354 MS.gene68376:CDS
CAAGTATAACCAAACTTTGA+AGG 0.420791 1.3:+27417965 MS.gene68376:CDS
GACTGAAATGGAGAGTCTTC+CGG 0.425050 1.3:+27416134 MS.gene68376:CDS
TTATTGTTTGTTGGAGTCTA+CGG 0.429758 1.3:+27415900 MS.gene68376:CDS
ATATGTTGGATGAATTTGTT+GGG 0.432927 1.3:+27416637 MS.gene68376:CDS
AACATAATTGGCCCCGTTGT+CGG 0.433126 1.3:-27416694 None:intergenic
CAGGGCCAAACAAAATCAAA+TGG 0.435077 1.3:+27415933 MS.gene68376:CDS
GATATGTTGGATGAATTTGT+TGG 0.440061 1.3:+27416636 MS.gene68376:CDS
GATAAGTATAGGGCTAGTTC+GGG 0.441052 1.3:+27417548 MS.gene68376:CDS
TTAATTGCATTTCAGCTGCT+TGG 0.448243 1.3:+27417692 MS.gene68376:intron
ATCAAGCAATGGTGGGAGTA+AGG 0.451264 1.3:+27417279 MS.gene68376:CDS
GAAGGTTGGTAAGTGGGAAA+AGG 0.452327 1.3:+27416106 MS.gene68376:CDS
TGTTGCTTCCTCTATTGGGC+TGG 0.453869 1.3:+27415798 MS.gene68376:CDS
CAAAATCAAATGGGAGTGAC+AGG 0.455222 1.3:+27415943 MS.gene68376:CDS
CATCATCCGTTGCATGCAGC+TGG 0.456532 1.3:+27417389 MS.gene68376:CDS
TCACCGGCATTAGAAGATAA+TGG 0.457229 1.3:-27415757 None:intergenic
CCATCAAAATCTACTGGAAA+GGG 0.459432 1.3:+27418236 MS.gene68376:CDS
AAGGTTGGTAAGTGGGAAAA+GGG 0.459998 1.3:+27416107 MS.gene68376:CDS
AGATTGGCTAGATGTTGAAC+TGG 0.460501 1.3:+27418127 MS.gene68376:CDS
ATCTTGAGCCTCATTTGAAA+CGG 0.462692 1.3:-27416052 None:intergenic
GAGTTTGACTGTTAGCTCAT+CGG 0.464921 1.3:+27417771 MS.gene68376:CDS
TAATAAAGAAGAAGAGGATG+AGG 0.465873 1.3:+27418406 MS.gene68376:CDS
GCAGCTCCATCAAAATCTAC+TGG 0.466548 1.3:+27418230 MS.gene68376:CDS
GCAGCACGACTTCAAGAGAT+TGG 0.466842 1.3:+27416084 MS.gene68376:CDS
CAATATATTCATCACTTTCA+CGG 0.470374 1.3:-27417992 None:intergenic
GAGTCTTCCGGCCACTAAGA+AGG 0.470821 1.3:+27416146 MS.gene68376:CDS
ATGATGATGATGATTTAGAT+TGG 0.471179 1.3:+27418111 MS.gene68376:CDS
CAGCTGCTTGGTGGAATAGG+TGG 0.474504 1.3:+27417704 MS.gene68376:intron
AGAAGTTGCCTTCTTACCCT+TGG 0.474519 1.3:-27417138 None:intergenic
AGCCTAAGTACTCTTATAAG+AGG 0.478383 1.3:-27417218 None:intergenic
TGGTTTGACCAGCCCAATAG+AGG 0.478495 1.3:-27415806 None:intergenic
GGATTGTTATAAAAGTGCTT+CGG 0.481643 1.3:-27417425 None:intergenic
TATTGTTTGTTGGAGTCTAC+GGG 0.483113 1.3:+27415901 MS.gene68376:CDS
CAATGTTCTTCCCTTTGTAT+CGG 0.485821 1.3:-27416523 None:intergenic
GGAAAGGGAAAGGGAAATCA+AGG 0.485934 1.3:+27418257 MS.gene68376:CDS
GGAAGATAATAAAGAAGAAG+AGG 0.486231 1.3:+27418400 MS.gene68376:CDS
CAAACTTTCTTCAATTGCAA+AGG 0.488401 1.3:+27417039 MS.gene68376:CDS
AAAGGGAAATCAAGGGGCAA+AGG 0.488992 1.3:+27418265 MS.gene68376:CDS
AAGGGAAATCAAGGGGCAAA+GGG 0.491418 1.3:+27418266 MS.gene68376:CDS
CATCCTATGTCAGGAAGGGT+TGG 0.491956 1.3:-27415826 None:intergenic
GAAAAGGGCAAGGACTGAAA+TGG 0.492542 1.3:+27416122 MS.gene68376:CDS
ACACAAGGAATTAGTCTTGT+CGG 0.492872 1.3:+27416927 MS.gene68376:CDS
ATTGCATTTCAGCTGCTTGG+TGG 0.497682 1.3:+27417695 MS.gene68376:intron
TGGCTCCTAGTGTTGAATAA+GGG 0.500293 1.3:-27418167 None:intergenic
GAGAAAGTTTCCAAGGGAAA+AGG 0.503992 1.3:+27418299 MS.gene68376:CDS
TTATACTTCCATCCTATGTC+AGG 0.505546 1.3:-27415835 None:intergenic
CCGAGCCCTTATTCAACACT+AGG 0.506076 1.3:+27418162 MS.gene68376:CDS
AATGATGGTGGAAGTGGAAG+TGG 0.506348 1.3:+27418440 MS.gene68376:CDS
CACATGAATGATGGTGGAAG+TGG 0.507359 1.3:+27418434 MS.gene68376:CDS
AGATTGGAAGGTTGGTAAGT+GGG 0.515155 1.3:+27416100 MS.gene68376:CDS
TCCAACTCTTTGTTAAGACA+TGG 0.516853 1.3:-27416423 None:intergenic
ACTTCAAGAGATTGGAAGGT+TGG 0.517951 1.3:+27416092 MS.gene68376:CDS
GAGGATACGCGTACATCTCC+TGG 0.518363 1.3:+27417605 MS.gene68376:CDS
CTGTCAACAATCTCAAGGAT+TGG 0.518832 1.3:-27417347 None:intergenic
GCTTCCTCAGAAGTGAAAAC+CGG 0.519907 1.3:+27416600 MS.gene68376:CDS
CCAGAGTTTATTACTGATTG+GGG 0.519928 1.3:+27418353 MS.gene68376:CDS
CGAAAGCTGTGATGTGCAGC+TGG 0.520750 1.3:+27416668 MS.gene68376:CDS
TTTGAATTTGTTGAGGGAGA+AGG 0.521056 1.3:+27416974 MS.gene68376:CDS
GGTGAAATCCGTTTCAAATG+AGG 0.523303 1.3:+27416044 MS.gene68376:CDS
AAAATCTACTGGAAAGGGAA+AGG 0.525200 1.3:+27418241 MS.gene68376:CDS
ACTGGAAAGGGAAAGGGAAA+GGG 0.526937 1.3:+27418248 MS.gene68376:CDS
CCCATGTCTTAACAAAGAGT+TGG 0.527534 1.3:+27416422 MS.gene68376:CDS
CTCTTAATAAACATAGTGCC+TGG 0.527784 1.3:-27416570 None:intergenic
AAATCTACTGGAAAGGGAAA+GGG 0.528332 1.3:+27418242 MS.gene68376:CDS
ACAAATTCAAAACCAAAGAA+TGG 0.528552 1.3:-27416962 None:intergenic
TAAAGCTTGCTTAACGACAC+TGG 0.531131 1.3:+27415972 MS.gene68376:CDS
CCCCAATCAGTAATAAACTC+TGG 0.533580 1.3:-27418353 None:intergenic
AAAGGGAAAGGGAAATCAAG+GGG 0.533778 1.3:+27418259 MS.gene68376:CDS
TACTGGAAAGGGAAAGGGAA+AGG 0.534955 1.3:+27418247 MS.gene68376:CDS
CCTAGTGTTGAATAAGGGCT+CGG 0.535922 1.3:-27418162 None:intergenic
TCCATCAAAATCTACTGGAA+AGG 0.541789 1.3:+27418235 MS.gene68376:CDS
GTATTACCCTTCTTAGTGGC+CGG 0.542772 1.3:-27416153 None:intergenic
GTTCAATAATGTCCGATGGT+TGG 0.542782 1.3:+27416499 MS.gene68376:CDS
TGACGAAGCCAAGGGTAAGA+AGG 0.542807 1.3:+27417130 MS.gene68376:CDS
TGGGTTTCTCAACTCATGAT+AGG 0.543800 1.3:-27416403 None:intergenic
TCCTTGAGATTGTTGACAGG+AGG 0.550735 1.3:+27417351 MS.gene68376:CDS
AAACCAACCCTTCCTGACAT+AGG 0.551809 1.3:+27415823 MS.gene68376:CDS
TTTCATATATCAAGCAATGG+TGG 0.551820 1.3:+27417271 MS.gene68376:CDS
AAAGGTGAGTGACGAAGCCA+AGG 0.551906 1.3:+27417121 MS.gene68376:CDS
CACGACTTCAAGAGATTGGA+AGG 0.552643 1.3:+27416088 MS.gene68376:CDS
CAAGGGGCAAAGGGAAAGCA+AGG 0.553519 1.3:+27418275 MS.gene68376:CDS
CAACCCTTCCTGACATAGGA+TGG 0.554812 1.3:+27415827 MS.gene68376:CDS
TTCATATATCAAGCAATGGT+GGG 0.555106 1.3:+27417272 MS.gene68376:CDS
GAGATTGGAAGGTTGGTAAG+TGG 0.556292 1.3:+27416099 MS.gene68376:CDS
ATATATTGATCCGGTGCTCA+TGG 0.558036 1.3:+27418007 MS.gene68376:CDS
CTTTGTATCGGTCCAACCAT+CGG 0.558962 1.3:-27416511 None:intergenic
AGGGCCAAACAAAATCAAAT+GGG 0.560257 1.3:+27415934 MS.gene68376:CDS
AGTCTTCCGGCCACTAAGAA+GGG 0.561768 1.3:+27416147 MS.gene68376:CDS
TGAGATTGTTGACAGGAGGT+GGG 0.563133 1.3:+27417355 MS.gene68376:CDS
GCAAGGCGAGAAAGTTTCCA+AGG 0.566101 1.3:+27418292 MS.gene68376:CDS
CGATGGTTGGACCGATACAA+AGG 0.566638 1.3:+27416512 MS.gene68376:CDS
GCACCATTATCTTCTAATGC+CGG 0.566676 1.3:+27415754 MS.gene68376:CDS
AGCTAATGAGGAAGCTAGAG+TGG 0.569343 1.3:+27416017 MS.gene68376:CDS
GATGATCCTGAAATTAATGC+TGG 0.573270 1.3:+27418029 MS.gene68376:CDS
CAATCCTTGAGATTGTTGAC+AGG 0.573892 1.3:+27417348 MS.gene68376:CDS
AAGTGATGAATATATTGATC+CGG 0.573947 1.3:+27417998 MS.gene68376:CDS
TGAGGAAGCTAGAGTGGCAA+AGG 0.574189 1.3:+27416023 MS.gene68376:CDS
GGATAATACTAATCAAATGT+TGG 0.574824 1.3:+27416443 MS.gene68376:CDS
CCAACTCTTTGTTAAGACAT+GGG 0.575520 1.3:-27416422 None:intergenic
GCTGCTTGGTGGAATAGGTG+GGG 0.578425 1.3:+27417706 MS.gene68376:intron
GATATATGAAACTCATTGCA+GGG 0.583289 1.3:-27417259 None:intergenic
TCTTATAAGAGTACTTAGGC+TGG 0.583735 1.3:+27417220 MS.gene68376:CDS
ACCTCCTGTCAACAATCTCA+AGG 0.585500 1.3:-27417352 None:intergenic
TATACTTATCCAAATCTGCA+TGG 0.585773 1.3:-27417535 None:intergenic
TTAGCTCATCGGGGTGTGAG+AGG 0.585792 1.3:+27417782 MS.gene68376:CDS
ACTTCCATCCTATGTCAGGA+AGG 0.587027 1.3:-27415831 None:intergenic
CTTCCATCCTATGTCAGGAA+GGG 0.587261 1.3:-27415830 None:intergenic
TCAAATGTTGGAGACTAACA+AGG 0.590739 1.3:+27416455 MS.gene68376:CDS
TCAGGATCATCCATGAGCAC+CGG 0.591007 1.3:-27418017 None:intergenic
CTCAACTCATGATAGGAAGG+AGG 0.595896 1.3:-27416396 None:intergenic
AGAGTTTATTACTGATTGGG+GGG 0.596589 1.3:+27418355 MS.gene68376:CDS
TCTATACAATGTGCTGCACA+TGG 0.597200 1.3:-27416855 None:intergenic
TTGTGCAGCAGAAGAATCAC+CGG 0.597962 1.3:-27415773 None:intergenic
GAGCCAAGCAACTTTGCAGA+AGG 0.597989 1.3:+27418184 MS.gene68376:CDS
TGGTAAGTGGGAAAAGGGCA+AGG 0.600199 1.3:+27416112 MS.gene68376:CDS
TTTCTCAACTCATGATAGGA+AGG 0.600435 1.3:-27416399 None:intergenic
GTTCACTTCTCAAGAGTGGT+CGG 0.601953 1.3:+27417094 MS.gene68376:CDS
GTGCAGCTGGTTACCGACAA+CGG 0.604313 1.3:+27416681 MS.gene68376:CDS
GAAAGGGAAAGGGAAATCAA+GGG 0.604555 1.3:+27418258 MS.gene68376:CDS
GAGGCAGAGCACATGAATGA+TGG 0.605850 1.3:+27418425 MS.gene68376:CDS
CAAGGGAAAAGGAAAGAAAG+TGG 0.607971 1.3:+27418310 MS.gene68376:CDS
TGAGGCTCAAGATGATGAAG+AGG 0.609931 1.3:+27416062 MS.gene68376:CDS
AAATGTCCAGCTGCATGCAA+CGG 0.610121 1.3:-27417395 None:intergenic
TGATTAGACATGGAGTCACA+AGG 0.610919 1.3:+27417009 MS.gene68376:CDS
TTGCTTAACGACACTGGAGG+AGG 0.611100 1.3:+27415978 MS.gene68376:CDS
CCACTCTTGAGAAGTGAACA+TGG 0.616214 1.3:-27417090 None:intergenic
GTAAAGAAGGTCGTAACACA+AGG 0.616375 1.3:+27416912 MS.gene68376:CDS
GATGGTTGGACCGATACAAA+GGG 0.622306 1.3:+27416513 MS.gene68376:CDS
ATGGAAGTATAACAGTGTAA+GGG 0.622725 1.3:+27415846 MS.gene68376:CDS
CAGAGTTTATTACTGATTGG+GGG 0.628928 1.3:+27418354 MS.gene68376:CDS
GAGATTGTTGACAGGAGGTG+GGG 0.629952 1.3:+27417356 MS.gene68376:CDS
GATGAAGTTCGTATTCGTGA+TGG 0.631886 1.3:+27418071 MS.gene68376:CDS
CCATGTTCACTTCTCAAGAG+TGG 0.631953 1.3:+27417090 MS.gene68376:CDS
GAGTTTCATATATCAAGCAA+TGG 0.636888 1.3:+27417268 MS.gene68376:CDS
TTTATGCTCTTAAAGCTATG+GGG 0.638889 1.3:+27417195 MS.gene68376:CDS
GAAATGGAGTTGATTAGACA+TGG 0.642103 1.3:+27416999 MS.gene68376:CDS
GCAGAGCACATGAATGATGG+TGG 0.658579 1.3:+27418428 MS.gene68376:CDS
GGTTGTTCAATAATGTCCGA+TGG 0.658787 1.3:+27416495 MS.gene68376:CDS
GTCTACGGGAGGAATTTCCA+GGG 0.658920 1.3:+27415915 MS.gene68376:CDS
GGCCTCTTATAAGAGTACTT+AGG 0.659644 1.3:+27417216 MS.gene68376:CDS
CAAGGCGAGAAAGTTTCCAA+GGG 0.661318 1.3:+27418293 MS.gene68376:CDS
TAGGAAGGAGGCTTCAAGTG+AGG 0.666996 1.3:-27416384 None:intergenic
TGTTTGTTGGAGTCTACGGG+AGG 0.672619 1.3:+27415904 MS.gene68376:CDS
AGAAGCAATAAGGCACAGAG+AGG 0.676918 1.3:+27417586 MS.gene68376:CDS
CTAGATGTTGAACTGGCGAG+TGG 0.684599 1.3:+27418134 MS.gene68376:CDS
AAGGTGAGTGACGAAGCCAA+GGG 0.690496 1.3:+27417122 MS.gene68376:CDS
TATGTTGGATGAATTTGTTG+GGG 0.695591 1.3:+27416638 MS.gene68376:CDS
GTTTGACTGTTAGCTCATCG+GGG 0.706140 1.3:+27417773 MS.gene68376:CDS
TTAATAAACATAGTGCCTGG+CGG 0.727544 1.3:-27416567 None:intergenic
GCAGCTGGTTACCGACAACG+GGG 0.734340 1.3:+27416683 MS.gene68376:CDS
AGCTTGCTTAACGACACTGG+AGG 0.743395 1.3:+27415975 MS.gene68376:CDS
AATGTTGGAGACTAACAAGG+CGG 0.771250 1.3:+27416458 MS.gene68376:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAAATTAAGAAAAAATT+TGG + chr1.3:27417302-27417321 MS.gene68376:CDS 10.0%
!!! TTGGAAAAATATTTTTGTAA+AGG + chr1.3:27416792-27416811 MS.gene68376:intron 15.0%
!! AAGTATTAAAGATAGAGATA+AGG + chr1.3:27416242-27416261 MS.gene68376:intron 20.0%
!! ATCAAAAAGTTTCAAATTGA+TGG + chr1.3:27416338-27416357 MS.gene68376:intron 20.0%
!! GTAATAATACATGATATGTT+TGG - chr1.3:27416759-27416778 None:intergenic 20.0%
!! TAAAAGAACATTAAATCCAA+TGG - chr1.3:27416186-27416205 None:intergenic 20.0%
!! TATTACGTTCTTTAATTGTT+TGG + chr1.3:27416773-27416792 MS.gene68376:intron 20.0%
!!! AAAAGCAATTTTTGATATGT+TGG + chr1.3:27416623-27416642 MS.gene68376:CDS 20.0%
! AAAAAAAGCGAGTTATATAC+TGG - chr1.3:27416287-27416306 None:intergenic 25.0%
! AAAAATTCCAAGAGTAAAGA+AGG + chr1.3:27416899-27416918 MS.gene68376:CDS 25.0%
! AAATTAATGCTGGTAGTTTA+TGG + chr1.3:27418039-27418058 MS.gene68376:CDS 25.0%
! AAGTGATGAATATATTGATC+CGG + chr1.3:27417998-27418017 MS.gene68376:CDS 25.0%
! ACAAATTCAAAACCAAAGAA+TGG - chr1.3:27416965-27416984 None:intergenic 25.0%
! AGATAAAAATGATGCGTTAT+TGG + chr1.3:27417454-27417473 MS.gene68376:CDS 25.0%
! CAATATATTCATCACTTTCA+CGG - chr1.3:27417995-27418014 None:intergenic 25.0%
! GATTTAATGCTAGAAAAGAT+TGG + chr1.3:27416876-27416895 MS.gene68376:CDS 25.0%
! GGATAATACTAATCAAATGT+TGG + chr1.3:27416443-27416462 MS.gene68376:CDS 25.0%
! GTAGTAAGTAACTTTAGATT+TGG - chr1.3:27417742-27417761 None:intergenic 25.0%
! TTTCATCTATAACCATTCTT+TGG + chr1.3:27416950-27416969 MS.gene68376:CDS 25.0%
!! AAATTGATGGTTGAAGAAAT+TGG + chr1.3:27416351-27416370 MS.gene68376:intron 25.0%
!! ATATGTTGGATGAATTTGTT+GGG + chr1.3:27416637-27416656 MS.gene68376:CDS 25.0%
!! ATGATGATGATGATTTAGAT+TGG + chr1.3:27418111-27418130 MS.gene68376:CDS 25.0%
!!! ACTCGCTTTTTTTATCTAAA+TGG + chr1.3:27416294-27416313 MS.gene68376:intron 25.0%
!!! ATTGATTTTAAGAGCTAATG+AGG + chr1.3:27416005-27416024 MS.gene68376:CDS 25.0%
!!! CATATCAAAAATTGCTTTTC+CGG - chr1.3:27416622-27416641 None:intergenic 25.0%
!!! TGGTTCTAATTTTTTGTCTT+AGG - chr1.3:27417073-27417092 None:intergenic 25.0%
!!! TTTGGTTTTGAATTTGTTGA+GGG + chr1.3:27416968-27416987 MS.gene68376:CDS 25.0%
AAAAATGATGCGTTATTGGA+TGG + chr1.3:27417458-27417477 MS.gene68376:CDS 30.0%
AAACTACCAGCATTAATTTC+AGG - chr1.3:27418038-27418057 None:intergenic 30.0%
ACATGAGATGTTGCAAAATT+TGG + chr1.3:27417935-27417954 MS.gene68376:CDS 30.0%
ACTAAGAAGGGTAATACAAA+AGG + chr1.3:27416159-27416178 MS.gene68376:intron 30.0%
ATCCAGAGTTTATTACTGAT+TGG + chr1.3:27418351-27418370 MS.gene68376:CDS 30.0%
ATGCAGATTTGGATAAGTAT+AGG + chr1.3:27417537-27417556 MS.gene68376:CDS 30.0%
CAAACTTTCTTCAATTGCAA+AGG + chr1.3:27417039-27417058 MS.gene68376:CDS 30.0%
CAAGTATAACCAAACTTTGA+AGG + chr1.3:27417965-27417984 MS.gene68376:CDS 30.0%
GAGTTTCATATATCAAGCAA+TGG + chr1.3:27417268-27417287 MS.gene68376:CDS 30.0%
GATATATGAAACTCATTGCA+GGG - chr1.3:27417262-27417281 None:intergenic 30.0%
GATATGTTGGATGAATTTGT+TGG + chr1.3:27416636-27416655 MS.gene68376:CDS 30.0%
GGAAGATAATAAAGAAGAAG+AGG + chr1.3:27418400-27418419 MS.gene68376:CDS 30.0%
GTTGAAGAAATTGGTCAATA+TGG + chr1.3:27416360-27416379 MS.gene68376:intron 30.0%
GTTTATGCTCTTAAAGCTAT+GGG + chr1.3:27417194-27417213 MS.gene68376:CDS 30.0%
TAATAAAGAAGAAGAGGATG+AGG + chr1.3:27418406-27418425 MS.gene68376:CDS 30.0%
TAGAAAGCTTTCTTCTTCAA+TGG + chr1.3:27417496-27417515 MS.gene68376:CDS 30.0%
TAGTTCGGGTAACTTTAAAT+TGG + chr1.3:27417562-27417581 MS.gene68376:CDS 30.0%
TATACTTATCCAAATCTGCA+TGG - chr1.3:27417538-27417557 None:intergenic 30.0%
TGATATATGAAACTCATTGC+AGG - chr1.3:27417263-27417282 None:intergenic 30.0%
TGCAGATTTGGATAAGTATA+GGG + chr1.3:27417538-27417557 MS.gene68376:CDS 30.0%
TGTTTATGCTCTTAAAGCTA+TGG + chr1.3:27417193-27417212 MS.gene68376:CDS 30.0%
TTAAATTGGCAGAAGCAATA+AGG + chr1.3:27417576-27417595 MS.gene68376:CDS 30.0%
TTCACTCCAAGAAAAGAAAT+AGG + chr1.3:27417907-27417926 MS.gene68376:CDS 30.0%
TTCATATATCAAGCAATGGT+GGG + chr1.3:27417272-27417291 MS.gene68376:CDS 30.0%
TTTATGCTCTTAAAGCTATG+GGG + chr1.3:27417195-27417214 MS.gene68376:CDS 30.0%
TTTCATATATCAAGCAATGG+TGG + chr1.3:27417271-27417290 MS.gene68376:CDS 30.0%
! AATGTTCTTTTAAACACCTG+AGG + chr1.3:27416194-27416213 MS.gene68376:intron 30.0%
! ATGGAAGTATAACAGTGTAA+GGG + chr1.3:27415846-27415865 MS.gene68376:CDS 30.0%
! TCCAGAGTTTATTACTGATT+GGG + chr1.3:27418352-27418371 MS.gene68376:CDS 30.0%
! TTATTGTTTGTTGGAGTCTA+CGG + chr1.3:27415900-27415919 MS.gene68376:CDS 30.0%
! TTTTGTATTACCCTTCTTAG+TGG - chr1.3:27416160-27416179 None:intergenic 30.0%
!! GCATCATTTTTATCTATTGC+TGG - chr1.3:27417449-27417468 None:intergenic 30.0%
!! GGATTGTTATAAAAGTGCTT+CGG - chr1.3:27417428-27417447 None:intergenic 30.0%
!! TATGTTGGATGAATTTGTTG+GGG + chr1.3:27416638-27416657 MS.gene68376:CDS 30.0%
!! TTCAAGCCTATTTCTTTTCT+TGG - chr1.3:27417916-27417935 None:intergenic 30.0%
!!! CTTTGGTTTTGAATTTGTTG+AGG + chr1.3:27416967-27416986 MS.gene68376:CDS 30.0%
AAAATCTACTGGAAAGGGAA+AGG + chr1.3:27418241-27418260 MS.gene68376:CDS 35.0%
AAATCTACTGGAAAGGGAAA+GGG + chr1.3:27418242-27418261 MS.gene68376:CDS 35.0%
AGCCTAAGTACTCTTATAAG+AGG - chr1.3:27417221-27417240 None:intergenic 35.0%
AGGGCCAAACAAAATCAAAT+GGG + chr1.3:27415934-27415953 MS.gene68376:CDS 35.0%
ATCTTGAGCCTCATTTGAAA+CGG - chr1.3:27416055-27416074 None:intergenic 35.0%
CAATGTTCTTCCCTTTGTAT+CGG - chr1.3:27416526-27416545 None:intergenic 35.0%
CACGTGTGATTATTGTTTGT+TGG + chr1.3:27415891-27415910 MS.gene68376:CDS 35.0%
CCAACTCTTTGTTAAGACAT+GGG - chr1.3:27416425-27416444 None:intergenic 35.0%
CCATCAAAATCTACTGGAAA+GGG + chr1.3:27418236-27418255 MS.gene68376:CDS 35.0%
CTCTTAATAAACATAGTGCC+TGG - chr1.3:27416573-27416592 None:intergenic 35.0%
GAAGAAAGTAGAAACCTTAC+CGG - chr1.3:27416724-27416743 None:intergenic 35.0%
GATGATCCTGAAATTAATGC+TGG + chr1.3:27418029-27418048 MS.gene68376:CDS 35.0%
GCTGGTAGTTTATGGTTATT+GGG + chr1.3:27418047-27418066 MS.gene68376:CDS 35.0%
TCAAATGTTGGAGACTAACA+AGG + chr1.3:27416455-27416474 MS.gene68376:CDS 35.0%
TCCAACTCTTTGTTAAGACA+TGG - chr1.3:27416426-27416445 None:intergenic 35.0%
TCCATCAAAATCTACTGGAA+AGG + chr1.3:27418235-27418254 MS.gene68376:CDS 35.0%
TGAAGAAAAAGAGGATGAAG+AGG + chr1.3:27418379-27418398 MS.gene68376:CDS 35.0%
TGCTGGTAGTTTATGGTTAT+TGG + chr1.3:27418046-27418065 MS.gene68376:CDS 35.0%
TTAATAAACATAGTGCCTGG+CGG - chr1.3:27416570-27416589 None:intergenic 35.0%
TTAATTGCATTTCAGCTGCT+TGG + chr1.3:27417692-27417711 MS.gene68376:intron 35.0%
TTATACTTCCATCCTATGTC+AGG - chr1.3:27415838-27415857 None:intergenic 35.0%
TTTCTCAACTCATGATAGGA+AGG - chr1.3:27416402-27416421 None:intergenic 35.0%
! AATCTGTTGCTTCCTCTATT+GGG + chr1.3:27415794-27415813 MS.gene68376:CDS 35.0%
! AGAGTTTATTACTGATTGGG+GGG + chr1.3:27418355-27418374 MS.gene68376:CDS 35.0%
! CAGAGTTTATTACTGATTGG+GGG + chr1.3:27418354-27418373 MS.gene68376:CDS 35.0%
! CATGGAGTCCAAAAGATTTT+TGG - chr1.3:27416840-27416859 None:intergenic 35.0%
! CCAGAGTTTATTACTGATTG+GGG + chr1.3:27418353-27418372 MS.gene68376:CDS 35.0%
! GAAATGGAGTTGATTAGACA+TGG + chr1.3:27416999-27417018 MS.gene68376:CDS 35.0%
! GATGGAAGTATAACAGTGTA+AGG + chr1.3:27415845-27415864 MS.gene68376:CDS 35.0%
! TATTGTTTGTTGGAGTCTAC+GGG + chr1.3:27415901-27415920 MS.gene68376:CDS 35.0%
! TGTTCTTCAAGTCGCATTTT+GGG + chr1.3:27417163-27417182 MS.gene68376:CDS 35.0%
! TTGTTCTTCAAGTCGCATTT+TGG + chr1.3:27417162-27417181 MS.gene68376:CDS 35.0%
!! ACACAAGGAATTAGTCTTGT+CGG + chr1.3:27416927-27416946 MS.gene68376:CDS 35.0%
!! CACTCCCATTTGATTTTGTT+TGG - chr1.3:27415941-27415960 None:intergenic 35.0%
!! TCTTATAAGAGTACTTAGGC+TGG + chr1.3:27417220-27417239 MS.gene68376:CDS 35.0%
!! TTTGAATTTGTTGAGGGAGA+AGG + chr1.3:27416974-27416993 MS.gene68376:CDS 35.0%
AAAGGGAAAGGGAAATCAAG+GGG + chr1.3:27418259-27418278 MS.gene68376:CDS 40.0%
AATGTTGGAGACTAACAAGG+CGG + chr1.3:27416458-27416477 MS.gene68376:CDS 40.0%
ACTTCAAGAGATTGGAAGGT+TGG + chr1.3:27416092-27416111 MS.gene68376:CDS 40.0%
AGTTTGACTGTTAGCTCATC+GGG + chr1.3:27417772-27417791 MS.gene68376:CDS 40.0%
ATATATTGATCCGGTGCTCA+TGG + chr1.3:27418007-27418026 MS.gene68376:CDS 40.0%
CAAAATCAAATGGGAGTGAC+AGG + chr1.3:27415943-27415962 MS.gene68376:CDS 40.0%
CAAGGGAAAAGGAAAGAAAG+TGG + chr1.3:27418310-27418329 MS.gene68376:CDS 40.0%
CAATCCTTGAGATTGTTGAC+AGG + chr1.3:27417348-27417367 MS.gene68376:CDS 40.0%
CAGGGCCAAACAAAATCAAA+TGG + chr1.3:27415933-27415952 MS.gene68376:CDS 40.0%
CCCATGTCTTAACAAAGAGT+TGG + chr1.3:27416422-27416441 MS.gene68376:CDS 40.0%
CCCCAATCAGTAATAAACTC+TGG - chr1.3:27418356-27418375 None:intergenic 40.0%
CGAAATAGTAGTCACTTACC+AGG - chr1.3:27417626-27417645 None:intergenic 40.0%
CTGTCAACAATCTCAAGGAT+TGG - chr1.3:27417350-27417369 None:intergenic 40.0%
CTTACCGGCCAAAACATAAT+TGG - chr1.3:27416709-27416728 None:intergenic 40.0%
GAAAGGGAAAGGGAAATCAA+GGG + chr1.3:27418258-27418277 MS.gene68376:CDS 40.0%
GAGAAAGTTTCCAAGGGAAA+AGG + chr1.3:27418299-27418318 MS.gene68376:CDS 40.0%
GAGTTTGACTGTTAGCTCAT+CGG + chr1.3:27417771-27417790 MS.gene68376:CDS 40.0%
GATGAAGTTCGTATTCGTGA+TGG + chr1.3:27418071-27418090 MS.gene68376:CDS 40.0%
GCACCATTATCTTCTAATGC+CGG + chr1.3:27415754-27415773 MS.gene68376:CDS 40.0%
GGGTAATACAAAAGGTCCAT+TGG + chr1.3:27416167-27416186 MS.gene68376:intron 40.0%
GGTGAAATCCGTTTCAAATG+AGG + chr1.3:27416044-27416063 MS.gene68376:CDS 40.0%
GGTTGTTCAATAATGTCCGA+TGG + chr1.3:27416495-27416514 MS.gene68376:CDS 40.0%
GTAAAGAAGGTCGTAACACA+AGG + chr1.3:27416912-27416931 MS.gene68376:CDS 40.0%
GTTACGACCTTCTTTACTCT+TGG - chr1.3:27416909-27416928 None:intergenic 40.0%
GTTCAATAATGTCCGATGGT+TGG + chr1.3:27416499-27416518 MS.gene68376:CDS 40.0%
TAAACACCTGAGGCAACATT+GGG + chr1.3:27416204-27416223 MS.gene68376:intron 40.0%
TCACCGGCATTAGAAGATAA+TGG - chr1.3:27415760-27415779 None:intergenic 40.0%
TCTATACAATGTGCTGCACA+TGG - chr1.3:27416858-27416877 None:intergenic 40.0%
TGATGACATCCATGCAGATT+TGG + chr1.3:27417526-27417545 MS.gene68376:CDS 40.0%
TGATTAGACATGGAGTCACA+AGG + chr1.3:27417009-27417028 MS.gene68376:CDS 40.0%
TGGGTTTCTCAACTCATGAT+AGG - chr1.3:27416406-27416425 None:intergenic 40.0%
TTAAACACCTGAGGCAACAT+TGG + chr1.3:27416203-27416222 MS.gene68376:intron 40.0%
! AGATTGGCTAGATGTTGAAC+TGG + chr1.3:27418127-27418146 MS.gene68376:CDS 40.0%
! CAATCTGTTGCTTCCTCTAT+TGG + chr1.3:27415793-27415812 MS.gene68376:CDS 40.0%
! CACGGTTTTCCTTCAAAGTT+TGG - chr1.3:27417977-27417996 None:intergenic 40.0%
! CACTTTCTTTCCTTTTCCCT+TGG - chr1.3:27418312-27418331 None:intergenic 40.0%
! CAGCGAGACCAAAAATCTTT+TGG + chr1.3:27416829-27416848 MS.gene68376:CDS 40.0%
! CCCTTTCCAGTAGATTTTGA+TGG - chr1.3:27418239-27418258 None:intergenic 40.0%
! TAAAGCTTGCTTAACGACAC+TGG + chr1.3:27415972-27415991 MS.gene68376:CDS 40.0%
! TGGCTCCTAGTGTTGAATAA+GGG - chr1.3:27418170-27418189 None:intergenic 40.0%
! TTGGCTCCTAGTGTTGAATA+AGG - chr1.3:27418171-27418190 None:intergenic 40.0%
! TTTTCCGGTTTTCACTTCTG+AGG - chr1.3:27416607-27416626 None:intergenic 40.0%
! TTTTTAATCAACTCTCCGCC+AGG + chr1.3:27416552-27416571 MS.gene68376:CDS 40.0%
!! AAGGTTGGTAAGTGGGAAAA+GGG + chr1.3:27416107-27416126 MS.gene68376:CDS 40.0%
!! AGATTGGAAGGTTGGTAAGT+GGG + chr1.3:27416100-27416119 MS.gene68376:CDS 40.0%
!! CATTTGATTTTGTTTGGCCC+TGG - chr1.3:27415935-27415954 None:intergenic 40.0%
!! GATAAGTATAGGGCTAGTTC+GGG + chr1.3:27417548-27417567 MS.gene68376:CDS 40.0%
!! GATGAATTTGTTGGGGAGAT+TGG + chr1.3:27416645-27416664 MS.gene68376:CDS 40.0%
!! GGATAAGTATAGGGCTAGTT+CGG + chr1.3:27417547-27417566 MS.gene68376:CDS 40.0%
!! GGCCTCTTATAAGAGTACTT+AGG + chr1.3:27417216-27417235 MS.gene68376:CDS 40.0%
!!! CAATTATGTTTTGGCCGGTA+AGG + chr1.3:27416707-27416726 MS.gene68376:intron 40.0%
AAACCAACCCTTCCTGACAT+AGG + chr1.3:27415823-27415842 MS.gene68376:CDS 45.0%
AAAGGGAAATCAAGGGGCAA+AGG + chr1.3:27418265-27418284 MS.gene68376:CDS 45.0%
AAATGTCCAGCTGCATGCAA+CGG - chr1.3:27417398-27417417 None:intergenic 45.0%
AACATAATTGGCCCCGTTGT+CGG - chr1.3:27416697-27416716 None:intergenic 45.0%
AAGGCGGAAGCAGACAAATA+TGG + chr1.3:27416474-27416493 MS.gene68376:CDS 45.0%
AAGGGAAATCAAGGGGCAAA+GGG + chr1.3:27418266-27418285 MS.gene68376:CDS 45.0%
ACCTCCTGTCAACAATCTCA+AGG - chr1.3:27417355-27417374 None:intergenic 45.0%
ACTGGAAAGGGAAAGGGAAA+GGG + chr1.3:27418248-27418267 MS.gene68376:CDS 45.0%
ACTTCCATCCTATGTCAGGA+AGG - chr1.3:27415834-27415853 None:intergenic 45.0%
AGAAGCAATAAGGCACAGAG+AGG + chr1.3:27417586-27417605 MS.gene68376:CDS 45.0%
AGAAGTTGCCTTCTTACCCT+TGG - chr1.3:27417141-27417160 None:intergenic 45.0%
AGCTAATGAGGAAGCTAGAG+TGG + chr1.3:27416017-27416036 MS.gene68376:CDS 45.0%
ATTGCATTTCAGCTGCTTGG+TGG + chr1.3:27417695-27417714 MS.gene68376:intron 45.0%
CAAGGCGAGAAAGTTTCCAA+GGG + chr1.3:27418293-27418312 MS.gene68376:CDS 45.0%
CACGACTTCAAGAGATTGGA+AGG + chr1.3:27416088-27416107 MS.gene68376:CDS 45.0%
CCACTCTTGAGAAGTGAACA+TGG - chr1.3:27417093-27417112 None:intergenic 45.0%
CCATGTTCACTTCTCAAGAG+TGG + chr1.3:27417090-27417109 MS.gene68376:CDS 45.0%
CTCAACTCATGATAGGAAGG+AGG - chr1.3:27416399-27416418 None:intergenic 45.0%
CTTCCATCCTATGTCAGGAA+GGG - chr1.3:27415833-27415852 None:intergenic 45.0%
GAAAAGGGCAAGGACTGAAA+TGG + chr1.3:27416122-27416141 MS.gene68376:CDS 45.0%
GACTGAAATGGAGAGTCTTC+CGG + chr1.3:27416134-27416153 MS.gene68376:CDS 45.0%
GATGGTTGGACCGATACAAA+GGG + chr1.3:27416513-27416532 MS.gene68376:CDS 45.0%
GCAGCTCCATCAAAATCTAC+TGG + chr1.3:27418230-27418249 MS.gene68376:CDS 45.0%
GCTTCCTCAGAAGTGAAAAC+CGG + chr1.3:27416600-27416619 MS.gene68376:CDS 45.0%
GGAAAGGGAAAGGGAAATCA+AGG + chr1.3:27418257-27418276 MS.gene68376:CDS 45.0%
GTATTACCCTTCTTAGTGGC+CGG - chr1.3:27416156-27416175 None:intergenic 45.0%
GTTCACTTCTCAAGAGTGGT+CGG + chr1.3:27417094-27417113 MS.gene68376:CDS 45.0%
GTTTGACTGTTAGCTCATCG+GGG + chr1.3:27417773-27417792 MS.gene68376:CDS 45.0%
TACTGGAAAGGGAAAGGGAA+AGG + chr1.3:27418247-27418266 MS.gene68376:CDS 45.0%
TCAAGAGTGGTCGGACTTAA+AGG + chr1.3:27417103-27417122 MS.gene68376:CDS 45.0%
TCCTTGAGATTGTTGACAGG+AGG + chr1.3:27417351-27417370 MS.gene68376:CDS 45.0%
TGAGATTGTTGACAGGAGGT+GGG + chr1.3:27417355-27417374 MS.gene68376:CDS 45.0%
TGAGGCTCAAGATGATGAAG+AGG + chr1.3:27416062-27416081 MS.gene68376:CDS 45.0%
TTGAGATTGTTGACAGGAGG+TGG + chr1.3:27417354-27417373 MS.gene68376:CDS 45.0%
TTGGGGGGATGAAGAAAAAG+AGG + chr1.3:27418370-27418389 MS.gene68376:CDS 45.0%
TTGTGCAGCAGAAGAATCAC+CGG - chr1.3:27415776-27415795 None:intergenic 45.0%
! ATCAAGCAATGGTGGGAGTA+AGG + chr1.3:27417279-27417298 MS.gene68376:CDS 45.0%
! CAACGGGGCCAATTATGTTT+TGG + chr1.3:27416698-27416717 MS.gene68376:CDS 45.0%
! CATTTTCCCAATGTTGCCTC+AGG - chr1.3:27416213-27416232 None:intergenic 45.0%
! CCTAGTGTTGAATAAGGGCT+CGG - chr1.3:27418165-27418184 None:intergenic 45.0%
! CTTTGTATCGGTCCAACCAT+CGG - chr1.3:27416514-27416533 None:intergenic 45.0%
! TTTCAGCTGCTTGGTGGAAT+AGG + chr1.3:27417701-27417720 MS.gene68376:intron 45.0%
!! AATGATGGTGGAAGTGGAAG+TGG + chr1.3:27418440-27418459 MS.gene68376:CDS 45.0%
!! CACATGAATGATGGTGGAAG+TGG + chr1.3:27418434-27418453 MS.gene68376:CDS 45.0%
!! GAAGGTTGGTAAGTGGGAAA+AGG + chr1.3:27416106-27416125 MS.gene68376:CDS 45.0%
!! GAGATTGGAAGGTTGGTAAG+TGG + chr1.3:27416099-27416118 MS.gene68376:CDS 45.0%
!! GTAGATTTTGATGGAGCTGC+AGG - chr1.3:27418230-27418249 None:intergenic 45.0%
!!! ATTTTTGGTCTCGCTGCTTG+AGG - chr1.3:27416825-27416844 None:intergenic 45.0%
AAAGGTGAGTGACGAAGCCA+AGG + chr1.3:27417121-27417140 MS.gene68376:CDS 50.0%
AAGGTGAGTGACGAAGCCAA+GGG + chr1.3:27417122-27417141 MS.gene68376:CDS 50.0%
AGTCTACGGGAGGAATTTCC+AGG + chr1.3:27415914-27415933 MS.gene68376:CDS 50.0%
AGTCTTCCGGCCACTAAGAA+GGG + chr1.3:27416147-27416166 MS.gene68376:CDS 50.0%
CAACCCTTCCTGACATAGGA+TGG + chr1.3:27415827-27415846 MS.gene68376:CDS 50.0%
CATCCTATGTCAGGAAGGGT+TGG - chr1.3:27415829-27415848 None:intergenic 50.0%
CCGAGCCCTTATTCAACACT+AGG + chr1.3:27418162-27418181 MS.gene68376:CDS 50.0%
CGATGGTTGGACCGATACAA+AGG + chr1.3:27416512-27416531 MS.gene68376:CDS 50.0%
CTAGATGTTGAACTGGCGAG+TGG + chr1.3:27418134-27418153 MS.gene68376:CDS 50.0%
CTGCCTTCTGCAAAGTTGCT+TGG - chr1.3:27418190-27418209 None:intergenic 50.0%
GAGATTGTTGACAGGAGGTG+GGG + chr1.3:27417356-27417375 MS.gene68376:CDS 50.0%
GAGCCAAGCAACTTTGCAGA+AGG + chr1.3:27418184-27418203 MS.gene68376:CDS 50.0%
GAGGCAGAGCACATGAATGA+TGG + chr1.3:27418425-27418444 MS.gene68376:CDS 50.0%
GCAAGGCGAGAAAGTTTCCA+AGG + chr1.3:27418292-27418311 MS.gene68376:CDS 50.0%
GCAGAGCACATGAATGATGG+TGG + chr1.3:27418428-27418447 MS.gene68376:CDS 50.0%
GCAGCACGACTTCAAGAGAT+TGG + chr1.3:27416084-27416103 MS.gene68376:CDS 50.0%
GTCTACGGGAGGAATTTCCA+GGG + chr1.3:27415915-27415934 MS.gene68376:CDS 50.0%
GTTGAGGGAGAAGGCTGAAA+TGG + chr1.3:27416983-27417002 MS.gene68376:CDS 50.0%
TAGGAAGGAGGCTTCAAGTG+AGG - chr1.3:27416387-27416406 None:intergenic 50.0%
TCAGGATCATCCATGAGCAC+CGG - chr1.3:27418020-27418039 None:intergenic 50.0%
TGACGAAGCCAAGGGTAAGA+AGG + chr1.3:27417130-27417149 MS.gene68376:CDS 50.0%
TGAGGAAGCTAGAGTGGCAA+AGG + chr1.3:27416023-27416042 MS.gene68376:CDS 50.0%
TGGTAAGTGGGAAAAGGGCA+AGG + chr1.3:27416112-27416131 MS.gene68376:CDS 50.0%
TGGTTTGACCAGCCCAATAG+AGG - chr1.3:27415809-27415828 None:intergenic 50.0%
TTGCTTAACGACACTGGAGG+AGG + chr1.3:27415978-27415997 MS.gene68376:CDS 50.0%
! AGCTGCTTGGTGGAATAGGT+GGG + chr1.3:27417705-27417724 MS.gene68376:intron 50.0%
! AGCTTGCTTAACGACACTGG+AGG + chr1.3:27415975-27415994 MS.gene68376:CDS 50.0%
! TGTTGCTTCCTCTATTGGGC+TGG + chr1.3:27415798-27415817 MS.gene68376:CDS 50.0%
! TGTTTGTTGGAGTCTACGGG+AGG + chr1.3:27415904-27415923 MS.gene68376:CDS 50.0%
!!! GGGGCCAATTATGTTTTGGC+CGG + chr1.3:27416702-27416721 MS.gene68376:CDS 50.0%
CAAGGGGCAAAGGGAAAGCA+AGG + chr1.3:27418275-27418294 MS.gene68376:CDS 55.0%
CATCATCCGTTGCATGCAGC+TGG + chr1.3:27417389-27417408 MS.gene68376:CDS 55.0%
CATCGGGGTGTGAGAGGAAT+TGG + chr1.3:27417788-27417807 MS.gene68376:CDS 55.0%
CGAAAGCTGTGATGTGCAGC+TGG + chr1.3:27416668-27416687 MS.gene68376:CDS 55.0%
GAGGATACGCGTACATCTCC+TGG + chr1.3:27417605-27417624 MS.gene68376:CDS 55.0%
GAGTCTTCCGGCCACTAAGA+AGG + chr1.3:27416146-27416165 MS.gene68376:CDS 55.0%
GTGCAGCTGGTTACCGACAA+CGG + chr1.3:27416681-27416700 MS.gene68376:CDS 55.0%
TGCAGCTGGTTACCGACAAC+GGG + chr1.3:27416682-27416701 MS.gene68376:CDS 55.0%
TTAGCTCATCGGGGTGTGAG+AGG + chr1.3:27417782-27417801 MS.gene68376:CDS 55.0%
! CAGCTGCTTGGTGGAATAGG+TGG + chr1.3:27417704-27417723 MS.gene68376:intron 55.0%
! GCTGCTTGGTGGAATAGGTG+GGG + chr1.3:27417706-27417725 MS.gene68376:intron 55.0%
GCAGCTGGTTACCGACAACG+GGG + chr1.3:27416683-27416702 MS.gene68376:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 27415733 27418490 27415733 ID=MS.gene68376
chr1.3 mRNA 27415733 27418490 27415733 ID=MS.gene68376.t1;Parent=MS.gene68376
chr1.3 exon 27415733 27416168 27415733 ID=MS.gene68376.t1.exon1;Parent=MS.gene68376.t1
chr1.3 CDS 27415733 27416168 27415733 ID=cds.MS.gene68376.t1;Parent=MS.gene68376.t1
chr1.3 exon 27416367 27416723 27416367 ID=MS.gene68376.t1.exon2;Parent=MS.gene68376.t1
chr1.3 CDS 27416367 27416723 27416367 ID=cds.MS.gene68376.t1;Parent=MS.gene68376.t1
chr1.3 exon 27416814 27417626 27416814 ID=MS.gene68376.t1.exon3;Parent=MS.gene68376.t1
chr1.3 CDS 27416814 27417626 27416814 ID=cds.MS.gene68376.t1;Parent=MS.gene68376.t1
chr1.3 exon 27417707 27417825 27417707 ID=MS.gene68376.t1.exon4;Parent=MS.gene68376.t1
chr1.3 CDS 27417707 27417825 27417707 ID=cds.MS.gene68376.t1;Parent=MS.gene68376.t1
chr1.3 exon 27417906 27418490 27417906 ID=MS.gene68376.t1.exon5;Parent=MS.gene68376.t1
chr1.3 CDS 27417906 27418490 27417906 ID=cds.MS.gene68376.t1;Parent=MS.gene68376.t1
Gene Sequence

>MS.gene68376

ATGGCAAGTAGTTCAGCTGCAGCACCATTATCTTCTAATGCCGGTGATTCTTCTGCTGCACAATCTGTTGCTTCCTCTATTGGGCTGGTCAAACCAACCCTTCCTGACATAGGATGGAAGTATAACAGTGTAAGGGACAAGACAAATATGAAAAAAGTCACGTGTGATTATTGTTTGTTGGAGTCTACGGGAGGAATTTCCAGGGCCAAACAAAATCAAATGGGAGTGACAGGAAACGTTAAAGCTTGCTTAACGACACTGGAGGAGGTGAAATTGATTTTAAGAGCTAATGAGGAAGCTAGAGTGGCAAAGGTGAAATCCGTTTCAAATGAGGCTCAAGATGATGAAGAGGCAGCACGACTTCAAGAGATTGGAAGGTTGGTAAGTGGGAAAAGGGCAAGGACTGAAATGGAGAGTCTTCCGGCCACTAAGAAGGGTAATACAAAAGGTCCATTGGATTTAATGTTCTTTTAAACACCTGAGGCAACATTGGGAAAATGAAAGCAAACAAGTATTAAAGATAGAGATAAGGAAGCAAGAGAAAATACTAACCAGTATATAACTCGCTTTTTTTATCTAAATGGAATTGCGTTCAACGTTGCAAAATCAAAAAGTTTCAAATTGATGGTTGAAGAAATTGGTCAATATGGTCCTCACTTGAAGCCTCCTTCCTATCATGAGTTGAGAAACCCATGTCTTAACAAAGAGTTGGATAATACTAATCAAATGTTGGAGACTAACAAGGCGGAAGCAGACAAATATGGTTGTTCAATAATGTCCGATGGTTGGACCGATACAAAGGGAAGAACATTGATTAATTTTTTAATCAACTCTCCGCCAGGCACTATGTTTATTAAGAGTATCGATGCTTCCTCAGAAGTGAAAACCGGAAAAGCAATTTTTGATATGTTGGATGAATTTGTTGGGGAGATTGGCGAAAGCTGTGATGTGCAGCTGGTTACCGACAACGGGGCCAATTATGTTTTGGCCGGTAAGGTTTCTACTTTCTTCAATTACTTTATGCCAAACATATCATGTATTATTACGTTCTTTAATTGTTTGGAAAAATATTTTTGTAAAGGTAAATTTCCTCAAGCAGCGAGACCAAAAATCTTTTGGACTCCATGTGCAGCACATTGTATAGATTTAATGCTAGAAAAGATTGGAAAAATTCCAAGAGTAAAGAAGGTCGTAACACAAGGAATTAGTCTTGTCGGTTTCATCTATAACCATTCTTTGGTTTTGAATTTGTTGAGGGAGAAGGCTGAAATGGAGTTGATTAGACATGGAGTCACAAGGTTTGCAACAAACTTTCTTCAATTGCAAAGGTTGCACAACCTAAGACAAAAAATTAGAACCATGTTCACTTCTCAAGAGTGGTCGGACTTAAAGGTGAGTGACGAAGCCAAGGGTAAGAAGGCAACTTCTATTGTTCTTCAAGTCGCATTTTGGGATGATATTGTTTATGCTCTTAAAGCTATGGGGCCTCTTATAAGAGTACTTAGGCTGGCTGATAATGAAAAAGTCCCTGCAATGAGTTTCATATATCAAGCAATGGTGGGAGTAAGGAATCAAATTAAGAAAAAATTTGGTGACAATGAAAGCAAGTACAAACCAATCCTTGAGATTGTTGACAGGAGGTGGGGTAATCAGCTACATCATCCGTTGCATGCAGCTGGACATTTTCTGAATCCGAAGCACTTTTATAACAATCCAGCAATAGATAAAAATGATGCGTTATTGGATGGCGTTTTTGAATGCATTAGAAAGCTTTCTTCTTCAATGGAAAATTCTGATGACATCCATGCAGATTTGGATAAGTATAGGGCTAGTTCGGGTAACTTTAAATTGGCAGAAGCAATAAGGCACAGAGAGGATACGCGTACATCTCCTGGTAAGTGACTACTATTTCGTAAAAAATTAGTCACATGATAAATTTTATATGTTAATGCAAATAATTTAATTGCATTTCAGCTGCTTGGTGGAATAGGTGGGGAGCAAAAACACCAAATCTAAAGTTACTTACTACTAAAATTTTGAGTTTGACTGTTAGCTCATCGGGGTGTGAGAGGAATTGGAGTACTTTTGAGCATGTACGTTCTTTATATTTAGAGTTTCATAATCATCTATCATTTAAATTTTAAACTAACATTTAAATTATAATTTGTAATAGATTCACTCCAAGAAAAGAAATAGGCTTGAACATGAGATGTTGCAAAATTTGGTTTTTGTCAAGTATAACCAAACTTTGAAGGAAAACCGTGAAAGTGATGAATATATTGATCCGGTGCTCATGGATGATCCTGAAATTAATGCTGGTAGTTTATGGTTATTGGGAGATGAAGTTCGTATTCGTGATGGTCAAAGTGATATTGTTTATGATGATGATGATTTAGATTGGCTAGATGTTGAACTGGCGAGTGGTGCTGCCGAGCCCTTATTCAACACTAGGAGCCAAGCAACTTTGCAGAAGGCAGCAGCTGCTGCTACAGCTCCTGCAGCTCCATCAAAATCTACTGGAAAGGGAAAGGGAAAGGGAAATCAAGGGGCAAAGGGAAAGCAAGGCGAGAAAGTTTCCAAGGGAAAAGGAAAGAAAGTGGTAGTAGTTGACGAACATGATCCAGAGTTTATTACTGATTGGGGGGATGAAGAAAAAGAGGATGAAGAGGAAGATAATAAAGAAGAAGAGGATGAGGCAGAGCACATGAATGATGGTGGAAGTGGAAGTGGTGATGATGAAGATTTTCATGATTATTGA
Protein sequence

>MS.gene68376.t1

MASSSAAAPLSSNAGDSSAAQSVASSIGLVKPTLPDIGWKYNSVRDKTNMKKVTCDYCLLESTGGISRAKQNQMGVTGNVKACLTTLEEVKLILRANEEARVAKVKSVSNEAQDDEEAARLQEIGRLVSGKRARTEMESLPATKKEIGQYGPHLKPPSYHELRNPCLNKELDNTNQMLETNKAEADKYGCSIMSDGWTDTKGRTLINFLINSPPGTMFIKSIDASSEVKTGKAIFDMLDEFVGEIGESCDVQLVTDNGANYVLAGKFPQAARPKIFWTPCAAHCIDLMLEKIGKIPRVKKVVTQGISLVGFIYNHSLVLNLLREKAEMELIRHGVTRFATNFLQLQRLHNLRQKIRTMFTSQEWSDLKVSDEAKGKKATSIVLQVAFWDDIVYALKAMGPLIRVLRLADNEKVPAMSFIYQAMVGVRNQIKKKFGDNESKYKPILEIVDRRWGNQLHHPLHAAGHFLNPKHFYNNPAIDKNDALLDGVFECIRKLSSSMENSDDIHADLDKYRASSGNFKLAEAIRHREDTRTSPAAWWNRWGAKTPNLKLLTTKILSLTVSSSGCERNWSTFEHIHSKKRNRLEHEMLQNLVFVKYNQTLKENRESDEYIDPVLMDDPEINAGSLWLLGDEVRIRDGQSDIVYDDDDLDWLDVELASGAAEPLFNTRSQATLQKAAAAATAPAAPSKSTGKGKGKGNQGAKGKQGEKVSKGKGKKVVVVDEHDPEFITDWGDEEKEDEEEDNKEEEDEAEHMNDGGSGSGDDEDFHDY