Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68415.t1 | XP_003609924.2 | 97.9 | 143 | 3 | 0 | 1 | 143 | 1 | 143 | 2.90E-66 | 261.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68415.t1 | F4IXW2 | 49.6 | 139 | 61 | 2 | 5 | 143 | 2 | 131 | 8.4e-22 | 104.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68415.t1 | G7JR54 | 97.9 | 143 | 3 | 0 | 1 | 143 | 1 | 143 | 2.1e-66 | 261.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049144 | MS.gene68415 | 0.801001 | 1.11E-48 | -1.69E-46 |
| MS.gene051795 | MS.gene68415 | 0.859601 | 3.70E-63 | -1.69E-46 |
| MS.gene051826 | MS.gene68415 | 0.859198 | 4.88E-63 | -1.69E-46 |
| MS.gene056301 | MS.gene68415 | 0.829782 | 4.25E-55 | -1.69E-46 |
| MS.gene056302 | MS.gene68415 | 0.862514 | 4.80E-64 | -1.69E-46 |
| MS.gene060347 | MS.gene68415 | 0.827828 | 1.26E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68415.t1 | MTR_4g124430 | 97.902 | 143 | 3 | 0 | 1 | 143 | 1 | 143 | 4.33e-90 | 286 |
| MS.gene68415.t1 | MTR_4g122770 | 46.269 | 67 | 35 | 1 | 1 | 67 | 1 | 66 | 2.82e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68415.t1 | AT3G43300 | 51.064 | 141 | 56 | 3 | 5 | 143 | 2 | 131 | 3.09e-30 | 114 |
| MS.gene68415.t1 | AT3G43300 | 51.064 | 141 | 56 | 3 | 5 | 143 | 2 | 131 | 3.15e-30 | 114 |
| MS.gene68415.t1 | AT3G43300 | 51.064 | 141 | 56 | 3 | 5 | 143 | 2 | 131 | 3.30e-30 | 114 |
Find 23 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATAATATTTCCACTATA+AGG | 0.267450 | 8.2:+9060967 | None:intergenic |
| CTGATAGCTTTGTGAAGTTC+AGG | 0.298814 | 8.2:+9061505 | None:intergenic |
| AGTTCCTTCAAGTGTATTCC+CGG | 0.376579 | 8.2:+9060930 | None:intergenic |
| AGTGTAAATGAAACTGAAAA+TGG | 0.399535 | 8.2:-9061066 | MS.gene68415:CDS |
| TACAATCTAAAGCAGGTTCT+AGG | 0.401485 | 8.2:+9060848 | None:intergenic |
| GTTTCAAGCGCAGCAGATAA+TGG | 0.432158 | 8.2:-9060997 | MS.gene68415:CDS |
| TGGAAATATTATTGAACTGT+TGG | 0.438424 | 8.2:-9060959 | MS.gene68415:CDS |
| ATTGAACTGTTGGCAAAAGC+CGG | 0.446359 | 8.2:-9060949 | MS.gene68415:CDS |
| AAGTTCAGGATACTTCTTGC+CGG | 0.462860 | 8.2:+9061519 | None:intergenic |
| TTTCAAGTTCTTTGTTTCAA+CGG | 0.485298 | 8.2:+9060873 | None:intergenic |
| TTGAACTGTTGGCAAAAGCC+GGG | 0.487872 | 8.2:-9060948 | MS.gene68415:CDS |
| AATGGTAGCAAGCCTTATAG+TGG | 0.496747 | 8.2:-9060979 | MS.gene68415:CDS |
| TCTATGCTCAAGGAATGTTC+CGG | 0.525685 | 8.2:-9061538 | MS.gene68415:CDS |
| TGCTCGAGTAACAAAACCTC+CGG | 0.539750 | 8.2:+9061567 | None:intergenic |
| AAAGCCGGGAATACACTTGA+AGG | 0.556363 | 8.2:-9060934 | MS.gene68415:CDS |
| AGCATTCGATTCTATGCTCA+AGG | 0.569810 | 8.2:-9061548 | MS.gene68415:CDS |
| TACCTCACAGATTCAGGTGA+CGG | 0.616087 | 8.2:+9061197 | None:intergenic |
| GTTTCAACGGCAAGACGAAG+TGG | 0.624431 | 8.2:+9060886 | None:intergenic |
| ACGTGGATACAATCTAAAGC+AGG | 0.628018 | 8.2:+9060841 | None:intergenic |
| TACACTTGAAGGAACTGATG+CGG | 0.651365 | 8.2:-9060923 | MS.gene68415:CDS |
| TAGCCAGAGAAAACAGAGTG+AGG | 0.653970 | 8.2:-9061234 | MS.gene68415:CDS |
| CACCGTCACCTGAATCTGTG+AGG | 0.683855 | 8.2:-9061199 | MS.gene68415:intron |
| AAAGCTATCAGCAATTACAC+AGG | 0.685230 | 8.2:-9061493 | MS.gene68415:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAAAAATATTAAAAACATA+TGG | + | chr8.2:9061282-9061301 | None:intergenic | 10.0% |
| !!! | ATGTTTTTAATATTTTTACT+CGG | - | chr8.2:9061283-9061302 | MS.gene68415:intron | 10.0% |
| !! | AGATAAGATAAGATAATTAA+CGG | - | chr8.2:9061054-9061073 | MS.gene68415:CDS | 15.0% |
| !! | TTATCTTATCTTATCTTATT+CGG | + | chr8.2:9061050-9061069 | None:intergenic | 15.0% |
| !! | TCAATAATATTTCCACTATA+AGG | + | chr8.2:9061462-9061481 | None:intergenic | 20.0% |
| ! | AAACGAAAAAGAATCAAATG+CGG | + | chr8.2:9061023-9061042 | None:intergenic | 25.0% |
| ! | AGTGTAAATGAAACTGAAAA+TGG | - | chr8.2:9061360-9061379 | MS.gene68415:intron | 25.0% |
| ! | TGGAAATATTATTGAACTGT+TGG | - | chr8.2:9061467-9061486 | MS.gene68415:intron | 25.0% |
| !! | ATTTTCAGTTTCATTTACAC+TGG | + | chr8.2:9061361-9061380 | None:intergenic | 25.0% |
| !!! | TTTCAAGTTCTTTGTTTCAA+CGG | + | chr8.2:9061556-9061575 | None:intergenic | 25.0% |
| AAAAATTACCTCACAGATTC+AGG | + | chr8.2:9061238-9061257 | None:intergenic | 30.0% | |
| !! | ATGAAAATTGATAGCGATCT+AGG | + | chr8.2:9060980-9060999 | None:intergenic | 30.0% |
| !! | TAATATTTTTACTCGGAGAG+TGG | - | chr8.2:9061290-9061309 | MS.gene68415:intron | 30.0% |
| !! | TGAAAATTGATAGCGATCTA+GGG | + | chr8.2:9060979-9060998 | None:intergenic | 30.0% |
| AAAGCTATCAGCAATTACAC+AGG | - | chr8.2:9060933-9060952 | MS.gene68415:CDS | 35.0% | |
| GTTTCATTGTTCAATTGTGC+TGG | - | chr8.2:9061076-9061095 | MS.gene68415:intron | 35.0% | |
| ! | TACAATCTAAAGCAGGTTCT+AGG | + | chr8.2:9061581-9061600 | None:intergenic | 35.0% |
| AAGTTCAGGATACTTCTTGC+CGG | + | chr8.2:9060910-9060929 | None:intergenic | 40.0% | |
| AGCATTCGATTCTATGCTCA+AGG | - | chr8.2:9060878-9060897 | MS.gene68415:CDS | 40.0% | |
| AGTTCCTTCAAGTGTATTCC+CGG | + | chr8.2:9061499-9061518 | None:intergenic | 40.0% | |
| ATTGAACTGTTGGCAAAAGC+CGG | - | chr8.2:9061477-9061496 | MS.gene68415:intron | 40.0% | |
| TACACTTGAAGGAACTGATG+CGG | - | chr8.2:9061503-9061522 | MS.gene68415:CDS | 40.0% | |
| TCTATGCTCAAGGAATGTTC+CGG | - | chr8.2:9060888-9060907 | MS.gene68415:CDS | 40.0% | |
| ! | AATGGTAGCAAGCCTTATAG+TGG | - | chr8.2:9061447-9061466 | MS.gene68415:intron | 40.0% |
| !! | CTGATAGCTTTGTGAAGTTC+AGG | + | chr8.2:9060924-9060943 | None:intergenic | 40.0% |
| !!! | TTTTCTCTGGCTAGCTTCTT+TGG | + | chr8.2:9061185-9061204 | None:intergenic | 40.0% |
| AAAGCCGGGAATACACTTGA+AGG | - | chr8.2:9061492-9061511 | MS.gene68415:intron | 45.0% | |
| GTTTCAAGCGCAGCAGATAA+TGG | - | chr8.2:9061429-9061448 | MS.gene68415:intron | 45.0% | |
| TACCTCACAGATTCAGGTGA+CGG | + | chr8.2:9061232-9061251 | None:intergenic | 45.0% | |
| TAGCCAGAGAAAACAGAGTG+AGG | - | chr8.2:9061192-9061211 | MS.gene68415:intron | 45.0% | |
| TGCTCGAGTAACAAAACCTC+CGG | + | chr8.2:9060862-9060881 | None:intergenic | 45.0% | |
| TTGAACTGTTGGCAAAAGCC+GGG | - | chr8.2:9061478-9061497 | MS.gene68415:intron | 45.0% | |
| ! | TAGCCTCACTCTGTTTTCTC+TGG | + | chr8.2:9061198-9061217 | None:intergenic | 45.0% |
| GTTTCAACGGCAAGACGAAG+TGG | + | chr8.2:9061543-9061562 | None:intergenic | 50.0% | |
| ! | TTTTACTCGGAGAGTGGACG+TGG | - | chr8.2:9061296-9061315 | MS.gene68415:intron | 50.0% |
| CACCGTCACCTGAATCTGTG+AGG | - | chr8.2:9061227-9061246 | MS.gene68415:CDS | 55.0% | |
| !! | ATGGCTGGGGGTGCTGCCGG+AGG | - | chr8.2:9060843-9060862 | MS.gene68415:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 9060843 | 9061605 | 9060843 | ID=MS.gene68415 |
| chr8.2 | mRNA | 9060843 | 9061605 | 9060843 | ID=MS.gene68415.t1;Parent=MS.gene68415 |
| chr8.2 | exon | 9061494 | 9061605 | 9061494 | ID=MS.gene68415.t1.exon1;Parent=MS.gene68415.t1 |
| chr8.2 | CDS | 9061494 | 9061605 | 9061494 | ID=cds.MS.gene68415.t1;Parent=MS.gene68415.t1 |
| chr8.2 | exon | 9061200 | 9061272 | 9061200 | ID=MS.gene68415.t1.exon2;Parent=MS.gene68415.t1 |
| chr8.2 | CDS | 9061200 | 9061272 | 9061200 | ID=cds.MS.gene68415.t1;Parent=MS.gene68415.t1 |
| chr8.2 | exon | 9060843 | 9061086 | 9060843 | ID=MS.gene68415.t1.exon3;Parent=MS.gene68415.t1 |
| chr8.2 | CDS | 9060843 | 9061086 | 9060843 | ID=cds.MS.gene68415.t1;Parent=MS.gene68415.t1 |
| Gene Sequence |
| Protein sequence |
