Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68816.t1 | XP_003626627.1 | 97.7 | 428 | 10 | 0 | 1 | 428 | 1 | 428 | 1.60E-240 | 841.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68816.t1 | Q1STP4 | 97.7 | 428 | 10 | 0 | 1 | 428 | 1 | 428 | 1.1e-240 | 841.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052307 | MS.gene68816 | 0.806489 | 8.02E-50 | -1.69E-46 |
| MS.gene052609 | MS.gene68816 | 0.823293 | 1.50E-53 | -1.69E-46 |
| MS.gene058639 | MS.gene68816 | 0.804824 | 1.79E-49 | -1.69E-46 |
| MS.gene059171 | MS.gene68816 | 0.811995 | 5.30E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68816.t1 | MTR_8g005040 | 97.664 | 428 | 10 | 0 | 1 | 428 | 1 | 428 | 0.0 | 868 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68816.t1 | AT5G01010 | 66.282 | 433 | 135 | 3 | 3 | 426 | 2 | 432 | 0.0 | 597 |
| MS.gene68816.t1 | AT5G01010 | 60.549 | 474 | 135 | 4 | 3 | 426 | 2 | 473 | 0.0 | 576 |
| MS.gene68816.t1 | AT5G01010 | 60.549 | 474 | 135 | 4 | 3 | 426 | 2 | 473 | 0.0 | 576 |
| MS.gene68816.t1 | AT5G01010 | 65.952 | 420 | 132 | 3 | 3 | 413 | 2 | 419 | 0.0 | 574 |
| MS.gene68816.t1 | AT5G01010 | 59.283 | 474 | 134 | 5 | 3 | 426 | 2 | 466 | 0.0 | 555 |
Find 97 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGAATTTCTGCACATTAA+TGG | 0.230921 | 8.4:+81721140 | MS.gene68816:intron |
| TCGTACTATGACCACTGATT+TGG | 0.264731 | 8.4:+81713968 | MS.gene68816:CDS |
| CTGGAAGCTATAAACTGATA+TGG | 0.273766 | 8.4:+81721163 | MS.gene68816:CDS |
| AGAGGTTCATCACATTTCTT+AGG | 0.283237 | 8.4:+81718330 | MS.gene68816:CDS |
| TTAGGATATTGGATTTAGTT+TGG | 0.287616 | 8.4:+81720741 | MS.gene68816:intron |
| TTTGTTTATTGTTAGGATAT+TGG | 0.304837 | 8.4:+81720730 | MS.gene68816:intron |
| CGTACTATGACCACTGATTT+GGG | 0.334259 | 8.4:+81713969 | MS.gene68816:CDS |
| AGTACAAGGCAAGATAAATA+TGG | 0.362025 | 8.4:+81720620 | MS.gene68816:CDS |
| TTCATTCCAGCAGATGTTGC+TGG | 0.364334 | 8.4:+81720132 | MS.gene68816:CDS |
| GGCTGCTTCTACAGAGGGTT+TGG | 0.370132 | 8.4:+81713851 | MS.gene68816:CDS |
| ATCAATCTTATGTGGAGGTT+GGG | 0.375986 | 8.4:-81714018 | None:intergenic |
| CGACAGGAAGGCCCTAGTTA+TGG | 0.377287 | 8.4:-81713877 | None:intergenic |
| CATCAATCTTATGTGGAGGT+TGG | 0.384125 | 8.4:-81714019 | None:intergenic |
| GATTCTTCCTTTCATTATCA+TGG | 0.387212 | 8.4:+81716592 | MS.gene68816:CDS |
| TTGCTGGTGCAGTGGAAATA+AGG | 0.393624 | 8.4:+81720148 | MS.gene68816:CDS |
| ACTGTAGGTGCTACGGTATA+AGG | 0.397024 | 8.4:+81721570 | MS.gene68816:intron |
| AATGGCACTGGCTCTGCAAC+AGG | 0.413130 | 8.4:-81721607 | None:intergenic |
| CTGGATTACATTACCGTGTC+TGG | 0.414338 | 8.4:-81714107 | None:intergenic |
| GCAAGCAGAGGTGGATGCTT+TGG | 0.423060 | 8.4:+81714415 | MS.gene68816:CDS |
| AGGGGAACAGTTAGCTGTCT+TGG | 0.427621 | 8.4:+81718274 | MS.gene68816:CDS |
| GTGGATGCTTTGGTAAATTC+TGG | 0.428219 | 8.4:+81714425 | MS.gene68816:CDS |
| GCTCAGCTTGGCTCTGCAAC+AGG | 0.429973 | 8.4:+81714512 | MS.gene68816:CDS |
| GGAGAAAGAGGAGGAGTGAA+GGG | 0.434405 | 8.4:-81713918 | None:intergenic |
| GACAGGAAGGCCCTAGTTAT+GGG | 0.435903 | 8.4:-81713876 | None:intergenic |
| TCATCGCACTTTGACCACTT+TGG | 0.439826 | 8.4:+81714277 | MS.gene68816:CDS |
| TCCGATGGCTGCTTCTACAG+AGG | 0.447189 | 8.4:+81713845 | None:intergenic |
| ACCCTCGTCAAGTACCTTGT+TGG | 0.452026 | 8.4:+81714545 | MS.gene68816:CDS |
| AGCTGTTGATGTCTACACTA+TGG | 0.459802 | 8.4:+81718301 | MS.gene68816:CDS |
| AGGGTTTGGTGCCCATAACT+AGG | 0.461857 | 8.4:+81713865 | MS.gene68816:CDS |
| GCGATGACGCTGCTCCTCCA+TGG | 0.464270 | 8.4:-81716523 | None:intergenic |
| ATTCTTCCTTTCATTATCAT+GGG | 0.475061 | 8.4:+81716593 | MS.gene68816:CDS |
| TTTGAGCTTCCCAAGAATAA+CGG | 0.475966 | 8.4:-81714246 | None:intergenic |
| GAAGTAAGGTGAGAGACGTC+TGG | 0.478606 | 8.4:-81713939 | None:intergenic |
| TTGCATCAGTCGAATGGCAC+TGG | 0.479589 | 8.4:-81721619 | None:intergenic |
| TTGGAGTTTGCAAGTCCTAC+CGG | 0.480664 | 8.4:+81720760 | MS.gene68816:CDS |
| ACTGCACCAGCAACATCTGC+TGG | 0.481617 | 8.4:-81720138 | None:intergenic |
| GTGATCGATATGCTCTTCTC+TGG | 0.488861 | 8.4:+81714459 | MS.gene68816:CDS |
| TGGAGAAAGAGGAGGAGTGA+AGG | 0.493800 | 8.4:-81713919 | None:intergenic |
| TGGAAGCTATAAACTGATAT+GGG | 0.497013 | 8.4:+81721164 | MS.gene68816:CDS |
| ACGGAAAATTGAATATTGTC+AGG | 0.498862 | 8.4:-81714227 | None:intergenic |
| ATGATAATGAAAGGAAGAAT+CGG | 0.504065 | 8.4:-81716590 | None:intergenic |
| TGAGAGACGTCTGGAGAAAG+AGG | 0.507695 | 8.4:-81713930 | None:intergenic |
| TAGTACGAAGCAGAGAAGTA+AGG | 0.514503 | 8.4:-81713953 | None:intergenic |
| GAGGAATAAGCAAGCAGAGG+TGG | 0.517070 | 8.4:+81714406 | MS.gene68816:CDS |
| AATTTCTGCACATTAATGGC+TGG | 0.520564 | 8.4:+81721144 | MS.gene68816:CDS |
| ATTGAGGTGAGGCTGTATAA+TGG | 0.524864 | 8.4:-81716555 | None:intergenic |
| CCGATGGCTGCTTCTACAGA+GGG | 0.525140 | 8.4:+81713846 | None:intergenic |
| GAGGAGCAGCGTCATCGCTA+TGG | 0.528258 | 8.4:+81716528 | MS.gene68816:CDS |
| CAGTAAACTCGTATATTGCA+TGG | 0.528695 | 8.4:+81720586 | MS.gene68816:CDS |
| CGATGACGCTGCTCCTCCAT+GGG | 0.529566 | 8.4:-81716522 | None:intergenic |
| AAAGAGATAAGTGTTCCAGC+TGG | 0.530707 | 8.4:+81720258 | MS.gene68816:CDS |
| TGGAGTTTGCAAGTCCTACC+GGG | 0.533936 | 8.4:+81720761 | MS.gene68816:CDS |
| TCTTCTCTGGAAACAACAAA+TGG | 0.535849 | 8.4:+81714472 | MS.gene68816:CDS |
| ATATCAGGTTATACTTTCAG+TGG | 0.541751 | 8.4:+81720560 | MS.gene68816:intron |
| CTTATCTACTGGTAGGCCCA+TGG | 0.549474 | 8.4:+81716506 | MS.gene68816:intron |
| GGCAGATTAATGACGATGAC+GGG | 0.550469 | 8.4:+81716347 | MS.gene68816:CDS |
| TCAGACTCGTATCGGCGGTA+AGG | 0.558609 | 8.4:-81721022 | None:intergenic |
| GGGTTTGGTGCCCATAACTA+GGG | 0.559944 | 8.4:+81713866 | MS.gene68816:CDS |
| GAGAAAGAGGAGGAGTGAAG+GGG | 0.566570 | 8.4:-81713917 | None:intergenic |
| ACATCTGCTGGAATGAAGAA+AGG | 0.566781 | 8.4:-81720126 | None:intergenic |
| AGAAGGCAATTAGCTCAGCT+TGG | 0.567507 | 8.4:+81714500 | MS.gene68816:CDS |
| AGCGTTCGCGTTGTTGTCTG+AGG | 0.569416 | 8.4:-81714379 | None:intergenic |
| TATAATGACTGTAGGTGCTA+CGG | 0.577224 | 8.4:+81721563 | MS.gene68816:intron |
| TCTGGAAACAACAAATGGAA+AGG | 0.577715 | 8.4:+81714477 | MS.gene68816:CDS |
| CTCCAACAAGGTACTTGACG+AGG | 0.578652 | 8.4:-81714547 | None:intergenic |
| AAGAGATAAGTGTTCCAGCT+GGG | 0.581420 | 8.4:+81720259 | MS.gene68816:CDS |
| ATCGTGCCCATGATAATGAA+AGG | 0.583994 | 8.4:-81716599 | None:intergenic |
| AACAACAAATGGAAAGGAGA+AGG | 0.585723 | 8.4:+81714483 | MS.gene68816:CDS |
| AGCAGATGTTGCTGGTGCAG+TGG | 0.597546 | 8.4:+81720140 | MS.gene68816:CDS |
| AAATTGAATATTGTCAGGTG+TGG | 0.598417 | 8.4:-81714222 | None:intergenic |
| GTTTGTCGTAGTAAGACGAC+AGG | 0.600262 | 8.4:-81713893 | None:intergenic |
| AGTAAACTCGTATATTGCAT+GGG | 0.604344 | 8.4:+81720587 | MS.gene68816:CDS |
| AGAGAAGAGCATATCGATCA+CGG | 0.605518 | 8.4:-81714457 | None:intergenic |
| TAACTTGGTCAGACTCGTAT+CGG | 0.609024 | 8.4:-81721030 | None:intergenic |
| TCTACACTATGGAGTTTGAG+AGG | 0.613870 | 8.4:+81718312 | MS.gene68816:CDS |
| CCCTCTGTAGAAGCAGCCAT+CGG | 0.614975 | 8.4:-81713846 | None:intergenic |
| GTACCTTGTTGGAGTTCCTG+AGG | 0.616247 | 8.4:+81714556 | MS.gene68816:CDS |
| AGGCAGATTAATGACGATGA+CGG | 0.621786 | 8.4:+81716346 | MS.gene68816:CDS |
| ACAATCTAATTGGCCAGACA+CGG | 0.626292 | 8.4:+81714094 | MS.gene68816:CDS |
| GGCACTGGCTCTGCAACAGG+AGG | 0.638970 | 8.4:-81721604 | None:intergenic |
| ATAAGATTGATGAAAACATG+TGG | 0.639942 | 8.4:+81714030 | MS.gene68816:CDS |
| ATCATCAATTCCCAAATCAG+TGG | 0.640263 | 8.4:-81713979 | None:intergenic |
| ATATTAATTTGTCAGAAAAG+GGG | 0.642676 | 8.4:+81718256 | MS.gene68816:intron |
| AGAGAGGAATAAGCAAGCAG+AGG | 0.647377 | 8.4:+81714403 | MS.gene68816:CDS |
| TCGGACAGAAAGAATCATTG+AGG | 0.651275 | 8.4:-81716571 | None:intergenic |
| CAGAAAGAATCATTGAGGTG+AGG | 0.658338 | 8.4:-81716566 | None:intergenic |
| GAGACGTCTGGAGAAAGAGG+AGG | 0.662195 | 8.4:-81713927 | None:intergenic |
| GTCGTAGTAAGACGACAGGA+AGG | 0.670921 | 8.4:-81713889 | None:intergenic |
| TCCAACAAGGTACTTGACGA+GGG | 0.671006 | 8.4:-81714546 | None:intergenic |
| AGAGTTCCTCGAAAATCTTG+TGG | 0.681900 | 8.4:-81714356 | None:intergenic |
| AACAACGCGAACGCTCAGAG+AGG | 0.685075 | 8.4:+81714387 | MS.gene68816:CDS |
| CTTGGTCAGACTCGTATCGG+CGG | 0.685715 | 8.4:-81721027 | None:intergenic |
| GGAGTTTGCAAGTCCTACCG+GGG | 0.692066 | 8.4:+81720762 | MS.gene68816:CDS |
| GATTTGGAAAGATTCCAAAG+TGG | 0.692886 | 8.4:-81714291 | None:intergenic |
| ATCTACTGGTAGGCCCATGG+AGG | 0.694772 | 8.4:+81716509 | MS.gene68816:intron |
| ATACCTCAGGAACTCCAACA+AGG | 0.706412 | 8.4:-81714559 | None:intergenic |
| GAGGTGAGGCTGTATAATGG+TGG | 0.725726 | 8.4:-81716552 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 81713849 | 81721636 | 81713849 | ID=MS.gene68816 |
| chr8.4 | mRNA | 81713849 | 81721636 | 81713849 | ID=MS.gene68816.t1;Parent=MS.gene68816 |
| chr8.4 | exon | 81713849 | 81714115 | 81713849 | ID=MS.gene68816.t1.exon1;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81713849 | 81714115 | 81713849 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81714206 | 81714577 | 81714206 | ID=MS.gene68816.t1.exon2;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81714206 | 81714577 | 81714206 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81716328 | 81716368 | 81716328 | ID=MS.gene68816.t1.exon3;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81716328 | 81716368 | 81716328 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81716521 | 81716637 | 81716521 | ID=MS.gene68816.t1.exon4;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81716521 | 81716637 | 81716521 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81718271 | 81718351 | 81718271 | ID=MS.gene68816.t1.exon5;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81718271 | 81718351 | 81718271 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81720107 | 81720169 | 81720107 | ID=MS.gene68816.t1.exon6;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81720107 | 81720169 | 81720107 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81720242 | 81720293 | 81720242 | ID=MS.gene68816.t1.exon7;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81720242 | 81720293 | 81720242 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81720567 | 81720641 | 81720567 | ID=MS.gene68816.t1.exon8;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81720567 | 81720641 | 81720567 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81720745 | 81720792 | 81720745 | ID=MS.gene68816.t1.exon9;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81720745 | 81720792 | 81720745 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81721013 | 81721048 | 81721013 | ID=MS.gene68816.t1.exon10;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81721013 | 81721048 | 81721013 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81721141 | 81721215 | 81721141 | ID=MS.gene68816.t1.exon11;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81721141 | 81721215 | 81721141 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| chr8.4 | exon | 81721577 | 81721636 | 81721577 | ID=MS.gene68816.t1.exon12;Parent=MS.gene68816.t1 |
| chr8.4 | CDS | 81721577 | 81721636 | 81721577 | ID=cds.MS.gene68816.t1;Parent=MS.gene68816.t1 |
| Gene Sequence |
| Protein sequence |
