AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68870.t1 MTR_4g132760 91.228 228 9 4 1 228 1 217 1.19e-136 382
MS.gene68870.t1 MTR_2g025080 53.589 209 66 5 1 201 1 186 3.47e-58 182
MS.gene68870.t1 MTR_8g013650 83.673 49 8 0 180 228 186 234 1.65e-26 102
MS.gene68870.t1 MTR_7g056407 44.231 104 39 2 143 228 237 339 7.27e-21 89.7
MS.gene68870.t1 MTR_3g065180 60.000 50 20 0 179 228 291 340 4.53e-17 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68870.t1 AT4G03965 46.667 240 85 6 1 228 1 209 2.05e-58 184
MS.gene68870.t1 AT4G22250 48.624 218 76 7 21 228 23 214 7.35e-54 172
MS.gene68870.t1 AT1G62370 40.506 237 99 7 1 228 1 204 1.51e-45 150
MS.gene68870.t1 AT3G25030 52.542 118 46 2 118 228 96 210 1.96e-34 122
MS.gene68870.t1 AT3G25030 52.542 118 46 2 118 228 136 250 8.40e-34 122
MS.gene68870.t1 AT3G25030 52.542 118 46 2 118 228 136 250 8.40e-34 122
MS.gene68870.t1 AT3G25030 52.542 118 46 2 118 228 136 250 8.40e-34 122
MS.gene68870.t1 AT4G13100 49.612 129 32 4 118 228 146 259 3.12e-29 110
MS.gene68870.t1 AT4G13100 49.612 129 32 4 118 228 146 259 3.12e-29 110
MS.gene68870.t1 AT4G13100 49.612 129 32 4 118 228 146 259 3.12e-29 110
MS.gene68870.t1 AT4G13100 49.612 129 32 4 118 228 146 259 3.12e-29 110
MS.gene68870.t1 AT4G13100 49.612 129 32 4 118 228 143 256 3.61e-29 110
MS.gene68870.t1 AT4G13100 48.837 129 30 4 118 228 146 256 4.56e-29 110
MS.gene68870.t1 AT4G13100 48.837 129 30 4 118 228 146 256 4.56e-29 110
MS.gene68870.t1 AT4G13100 48.837 129 30 4 118 228 155 265 6.75e-29 109
MS.gene68870.t1 AT3G07120 59.184 49 20 0 180 228 312 360 7.07e-17 78.6

Find 76 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGTTCGTTGCGGATAAT+TGG 0.249983 8.2:+1497207 None:intergenic
GTTTCACATTCCGGTTCAAC+TGG 0.280636 8.2:+1497090 None:intergenic
GTGGTGGGTTCGTCTGGTTC+TGG 0.300899 8.2:-1497063 MS.gene68870:CDS
TTCTTTGAGACTACGTCTTC+TGG 0.310742 8.2:+1497281 None:intergenic
TGGGTTGTTGTGGTGCCACC+TGG 0.326153 8.2:-1497239 MS.gene68870:CDS
GAGGCTAGTGGCGGAGGCGG+AGG 0.344398 8.2:-1496988 MS.gene68870:CDS
ACTCCGGTGGTGGGTTCGTC+TGG 0.370885 8.2:-1497069 MS.gene68870:CDS
CCTTGCGGCGGCGTTGGCGG+CGG 0.381107 8.2:-1497034 MS.gene68870:CDS
CAAATGGACAGTTTAGATTC+AGG 0.383260 8.2:-1497309 MS.gene68870:CDS
CGCTTCAGATTCACCGGTAT+GGG 0.387954 8.2:-1497258 MS.gene68870:CDS
CGGTTTATTAACCGAACAAA+TGG 0.401011 8.2:-1497325 MS.gene68870:CDS
CCGCCGCCAACGCCGCCGCA+AGG 0.401311 8.2:+1497034 None:intergenic
CATTCCGGTTCAACTGGGTT+CGG 0.404037 8.2:+1497096 None:intergenic
ATCTAAACTGTCCATTTGTT+CGG 0.404178 8.2:+1497314 None:intergenic
GAACCTTGCGGCGGCGTTGG+CGG 0.404942 8.2:-1497037 MS.gene68870:CDS
TTCGGTTAATAAACCGAGAA+TGG 0.411730 8.2:+1497332 None:intergenic
GCTAGTGGCGGAGGCGGAGG+CGG 0.412142 8.2:-1496985 MS.gene68870:CDS
TCAACTCGGTCTCATATTGC+AGG 0.414710 8.2:-1497385 MS.gene68870:CDS
AGAGAATCAAGAACCATTCT+CGG 0.424957 8.2:-1497345 MS.gene68870:CDS
TTCAGTGTGCTGCGTGTGCA+TGG 0.441328 8.2:-1496860 MS.gene68870:CDS
GGCGGAGGCGGAGGCGGAGA+AGG 0.447052 8.2:-1496979 MS.gene68870:CDS
ACGGAGGAGGCTAGTGGCGG+AGG 0.451095 8.2:-1496994 MS.gene68870:CDS
AGATCCGAACCCAGTTGAAC+CGG 0.458025 8.2:-1497100 MS.gene68870:CDS
AATGAACCTTGCGGCGGCGT+TGG 0.463100 8.2:-1497040 MS.gene68870:CDS
ACCACCGGAGTTTCACATTC+CGG 0.463442 8.2:+1497081 None:intergenic
GAGGAGGCTAGTGGCGGAGG+CGG 0.471756 8.2:-1496991 MS.gene68870:CDS
TAATGCTGGTGCGGTGGTTG+TGG 0.471853 8.2:-1496893 MS.gene68870:CDS
TGGCGGCGGAGCGGCAAATG+AGG 0.476419 8.2:-1497020 MS.gene68870:CDS
GAGAATAGAACGATTACAAA+GGG 0.479329 8.2:+1496747 None:intergenic
CGGCGGCGTTGGCGGCGGAG+CGG 0.486064 8.2:-1497029 MS.gene68870:CDS
ACGCTTCAGATTCACCGGTA+TGG 0.495940 8.2:-1497259 MS.gene68870:CDS
GCTGGTGCGGTGGTTGTGGA+AGG 0.512004 8.2:-1496889 MS.gene68870:CDS
AGTTTCGTTGATGAAACTGT+TGG 0.512656 8.2:-1496941 MS.gene68870:CDS
CAACAACAGCAAAACGAAGA+AGG 0.516607 8.2:-1497171 MS.gene68870:CDS
GATGAAACTGTTGGAGGAAA+CGG 0.519664 8.2:-1496932 MS.gene68870:CDS
TTCGTTGATGAAACTGTTGG+AGG 0.522016 8.2:-1496938 MS.gene68870:CDS
GGAATGTGAAACTCCGGTGG+TGG 0.529577 8.2:-1497079 MS.gene68870:CDS
ACTGTTGGAGGAAACGGAAG+TGG 0.532838 8.2:-1496926 MS.gene68870:CDS
GCAAATGAGGGTAACGGAGG+AGG 0.537713 8.2:-1497007 MS.gene68870:CDS
GGCACCACAACAACCCATAC+CGG 0.553017 8.2:+1497245 None:intergenic
GGGTTGTTGTGGTGCCACCT+GGG 0.563855 8.2:-1497238 MS.gene68870:CDS
CGAAGAAGGTGGAGCTACAG+TGG 0.576742 8.2:-1497157 MS.gene68870:CDS
CCGGTCAATATGAGTCAACT+CGG 0.576822 8.2:-1497399 None:intergenic
TTTCACATTCCGGTTCAACT+GGG 0.578697 8.2:+1497091 None:intergenic
GAACCAGACGAACCCACCAC+CGG 0.580808 8.2:+1497066 None:intergenic
GTGTGCATGGGAAGAAAGAA+AGG 0.583290 8.2:-1496847 MS.gene68870:CDS
GTGGAAAATGAGAGTAATGC+TGG 0.585452 8.2:-1496907 MS.gene68870:CDS
AAAGAACGCTTCAGATTCAC+CGG 0.587827 8.2:-1497264 MS.gene68870:CDS
GGCGGCGGAGCGGCAAATGA+GGG 0.590263 8.2:-1497019 MS.gene68870:CDS
GCGGAGAAGGAGCGACGCCG+TGG 0.591678 8.2:-1496966 MS.gene68870:CDS
TCAGTGTGCTGCGTGTGCAT+GGG 0.593233 8.2:-1496859 MS.gene68870:CDS
CGAGAATAGAACGATTACAA+AGG 0.598192 8.2:+1496746 None:intergenic
CTGGTGCGGTGGTTGTGGAA+GGG 0.603127 8.2:-1496888 MS.gene68870:CDS
TTCACCGGTATGGGTTGTTG+TGG 0.607708 8.2:-1497249 MS.gene68870:CDS
GGAGCGGCAAATGAGGGTAA+CGG 0.608763 8.2:-1497013 MS.gene68870:CDS
CAACAGCAAAACGAAGAAGG+TGG 0.612798 8.2:-1497168 MS.gene68870:CDS
GTAACGGAGGAGGCTAGTGG+CGG 0.613385 8.2:-1496997 MS.gene68870:CDS
TGAACCGGAATGTGAAACTC+CGG 0.616093 8.2:-1497085 MS.gene68870:CDS
AGGGTAACGGAGGAGGCTAG+TGG 0.623269 8.2:-1497000 MS.gene68870:CDS
GTTCGTTGCGGATAATTGGA+CGG 0.625036 8.2:+1497211 None:intergenic
TTTGAGACTACGTCTTCTGG+TGG 0.634831 8.2:+1497284 None:intergenic
GAATGTGAAACTCCGGTGGT+GGG 0.635237 8.2:-1497078 MS.gene68870:CDS
GTTGATGATGATGTTCGTTG+CGG 0.636681 8.2:+1497199 None:intergenic
GCGGCAAATGAGGGTAACGG+AGG 0.646014 8.2:-1497010 MS.gene68870:CDS
GATAATTGGACGGAAAACCC+AGG 0.649559 8.2:+1497221 None:intergenic
AGGGAACTGTGGTTGAATCG+AGG 0.655246 8.2:-1496778 MS.gene68870:CDS
TTCATCAACGAAACTCTCCA+CGG 0.677889 8.2:+1496949 None:intergenic
TGAGAGTAATGCTGGTGCGG+TGG 0.688076 8.2:-1496899 MS.gene68870:CDS
GAGTGTGTTCGAGGGAACTG+TGG 0.692857 8.2:-1496789 MS.gene68870:CDS
TTCTGGAATGAACCTTGCGG+CGG 0.694080 8.2:-1497046 MS.gene68870:CDS
AAATGAGAGTAATGCTGGTG+CGG 0.699341 8.2:-1496902 MS.gene68870:CDS
GGTGCAGCGTTCATACCGTG+TGG 0.706510 8.2:-1496826 MS.gene68870:CDS
AGAATAGAACGATTACAAAG+GGG 0.720178 8.2:+1496748 None:intergenic
TGGTTCTGGAATGAACCTTG+CGG 0.740354 8.2:-1497049 MS.gene68870:CDS
ACCGGAATGTGAAACTCCGG+TGG 0.748395 8.2:-1497082 MS.gene68870:CDS
AATTGGACGGAAAACCCAGG+TGG 0.782059 8.2:+1497224 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAATAGAACGATTACAAAG+GGG + chr8.2:1497371-1497390 None:intergenic 30.0%
ATCTAAACTGTCCATTTGTT+CGG + chr8.2:1496805-1496824 None:intergenic 30.0%
GAGAATAGAACGATTACAAA+GGG + chr8.2:1497372-1497391 None:intergenic 30.0%
AGAGAATCAAGAACCATTCT+CGG - chr8.2:1496771-1496790 MS.gene68870:CDS 35.0%
CAAATGGACAGTTTAGATTC+AGG - chr8.2:1496807-1496826 MS.gene68870:CDS 35.0%
CGAGAATAGAACGATTACAA+AGG + chr8.2:1497373-1497392 None:intergenic 35.0%
CGGTTTATTAACCGAACAAA+TGG - chr8.2:1496791-1496810 MS.gene68870:CDS 35.0%
TTCGGTTAATAAACCGAGAA+TGG + chr8.2:1496787-1496806 None:intergenic 35.0%
! AGTTTCGTTGATGAAACTGT+TGG - chr8.2:1497175-1497194 MS.gene68870:CDS 35.0%
AAAGAACGCTTCAGATTCAC+CGG - chr8.2:1496852-1496871 MS.gene68870:CDS 40.0%
AAATGAGAGTAATGCTGGTG+CGG - chr8.2:1497214-1497233 MS.gene68870:CDS 40.0%
CAACAACAGCAAAACGAAGA+AGG - chr8.2:1496945-1496964 MS.gene68870:CDS 40.0%
GATGAAACTGTTGGAGGAAA+CGG - chr8.2:1497184-1497203 MS.gene68870:CDS 40.0%
GTGGAAAATGAGAGTAATGC+TGG - chr8.2:1497209-1497228 MS.gene68870:CDS 40.0%
TGATGTTCGTTGCGGATAAT+TGG + chr8.2:1496912-1496931 None:intergenic 40.0%
TTCATCAACGAAACTCTCCA+CGG + chr8.2:1497170-1497189 None:intergenic 40.0%
TTTCACATTCCGGTTCAACT+GGG + chr8.2:1497028-1497047 None:intergenic 40.0%
! TTCGTTGATGAAACTGTTGG+AGG - chr8.2:1497178-1497197 MS.gene68870:CDS 40.0%
! TTTTTGCAGAGTGTGTTCGA+GGG - chr8.2:1497319-1497338 MS.gene68870:CDS 40.0%
!! GTTGATGATGATGTTCGTTG+CGG + chr8.2:1496920-1496939 None:intergenic 40.0%
!! TTCTTTGAGACTACGTCTTC+TGG + chr8.2:1496838-1496857 None:intergenic 40.0%
CAACAGCAAAACGAAGAAGG+TGG - chr8.2:1496948-1496967 MS.gene68870:CDS 45.0%
GATAATTGGACGGAAAACCC+AGG + chr8.2:1496898-1496917 None:intergenic 45.0%
GTGTGCATGGGAAGAAAGAA+AGG - chr8.2:1497269-1497288 MS.gene68870:CDS 45.0%
GTTCGTTGCGGATAATTGGA+CGG + chr8.2:1496908-1496927 None:intergenic 45.0%
GTTTCACATTCCGGTTCAAC+TGG + chr8.2:1497029-1497048 None:intergenic 45.0%
TCAACTCGGTCTCATATTGC+AGG - chr8.2:1496731-1496750 MS.gene68870:CDS 45.0%
TGAACCGGAATGTGAAACTC+CGG - chr8.2:1497031-1497050 MS.gene68870:CDS 45.0%
! CTGCAAAAAGTGTGTCCACA+CGG + chr8.2:1497308-1497327 None:intergenic 45.0%
! CTTTTTGCAGAGTGTGTTCG+AGG - chr8.2:1497318-1497337 MS.gene68870:CDS 45.0%
! TTTGAGACTACGTCTTCTGG+TGG + chr8.2:1496835-1496854 None:intergenic 45.0%
!! TGGTTCTGGAATGAACCTTG+CGG - chr8.2:1497067-1497086 MS.gene68870:CDS 45.0%
AATTGGACGGAAAACCCAGG+TGG + chr8.2:1496895-1496914 None:intergenic 50.0%
ACCACCGGAGTTTCACATTC+CGG + chr8.2:1497038-1497057 None:intergenic 50.0%
ACGCTTCAGATTCACCGGTA+TGG - chr8.2:1496857-1496876 MS.gene68870:CDS 50.0%
ACTGTTGGAGGAAACGGAAG+TGG - chr8.2:1497190-1497209 MS.gene68870:CDS 50.0%
AGATCCGAACCCAGTTGAAC+CGG - chr8.2:1497016-1497035 MS.gene68870:CDS 50.0%
AGGGAACTGTGGTTGAATCG+AGG - chr8.2:1497338-1497357 MS.gene68870:CDS 50.0%
CATTCCGGTTCAACTGGGTT+CGG + chr8.2:1497023-1497042 None:intergenic 50.0%
CGCTTCAGATTCACCGGTAT+GGG - chr8.2:1496858-1496877 MS.gene68870:CDS 50.0%
GAATGTGAAACTCCGGTGGT+GGG - chr8.2:1497038-1497057 MS.gene68870:CDS 50.0%
! TTCACCGGTATGGGTTGTTG+TGG - chr8.2:1496867-1496886 MS.gene68870:CDS 50.0%
!! TTCTGGAATGAACCTTGCGG+CGG - chr8.2:1497070-1497089 MS.gene68870:CDS 50.0%
ACCGGAATGTGAAACTCCGG+TGG - chr8.2:1497034-1497053 MS.gene68870:CDS 55.0%
CGAAGAAGGTGGAGCTACAG+TGG - chr8.2:1496959-1496978 MS.gene68870:CDS 55.0%
GAGTGTGTTCGAGGGAACTG+TGG - chr8.2:1497327-1497346 MS.gene68870:CDS 55.0%
GGAATGTGAAACTCCGGTGG+TGG - chr8.2:1497037-1497056 MS.gene68870:CDS 55.0%
GGAGCGGCAAATGAGGGTAA+CGG - chr8.2:1497103-1497122 MS.gene68870:CDS 55.0%
GGCACCACAACAACCCATAC+CGG + chr8.2:1496874-1496893 None:intergenic 55.0%
TAATGCTGGTGCGGTGGTTG+TGG - chr8.2:1497223-1497242 MS.gene68870:CDS 55.0%
TCAGTGTGCTGCGTGTGCAT+GGG - chr8.2:1497257-1497276 MS.gene68870:CDS 55.0%
TGAGAGTAATGCTGGTGCGG+TGG - chr8.2:1497217-1497236 MS.gene68870:CDS 55.0%
TTCAGTGTGCTGCGTGTGCA+TGG - chr8.2:1497256-1497275 MS.gene68870:CDS 55.0%
!! GCAAATGAGGGTAACGGAGG+AGG - chr8.2:1497109-1497128 MS.gene68870:CDS 55.0%
AATGAACCTTGCGGCGGCGT+TGG - chr8.2:1497076-1497095 MS.gene68870:CDS 60.0%
CTGGTGCGGTGGTTGTGGAA+GGG - chr8.2:1497228-1497247 MS.gene68870:CDS 60.0%
GAACCAGACGAACCCACCAC+CGG + chr8.2:1497053-1497072 None:intergenic 60.0%
GGTGCAGCGTTCATACCGTG+TGG - chr8.2:1497290-1497309 MS.gene68870:CDS 60.0%
! GCGGCAAATGAGGGTAACGG+AGG - chr8.2:1497106-1497125 MS.gene68870:CDS 60.0%
! GTAACGGAGGAGGCTAGTGG+CGG - chr8.2:1497119-1497138 MS.gene68870:CDS 60.0%
!! AGGGTAACGGAGGAGGCTAG+TGG - chr8.2:1497116-1497135 MS.gene68870:CDS 60.0%
!! GGGTTGTTGTGGTGCCACCT+GGG - chr8.2:1496878-1496897 MS.gene68870:CDS 60.0%
!! GTGGTGGGTTCGTCTGGTTC+TGG - chr8.2:1497053-1497072 MS.gene68870:CDS 60.0%
!! TGGGTTGTTGTGGTGCCACC+TGG - chr8.2:1496877-1496896 MS.gene68870:CDS 60.0%
GCTGGTGCGGTGGTTGTGGA+AGG - chr8.2:1497227-1497246 MS.gene68870:CDS 65.0%
!! ACTCCGGTGGTGGGTTCGTC+TGG - chr8.2:1497047-1497066 MS.gene68870:CDS 65.0%
ACGGAGGAGGCTAGTGGCGG+AGG - chr8.2:1497122-1497141 MS.gene68870:CDS 70.0%
GGCGGCGGAGCGGCAAATGA+GGG - chr8.2:1497097-1497116 MS.gene68870:CDS 70.0%
TGGCGGCGGAGCGGCAAATG+AGG - chr8.2:1497096-1497115 MS.gene68870:CDS 70.0%
! GAACCTTGCGGCGGCGTTGG+CGG - chr8.2:1497079-1497098 MS.gene68870:CDS 70.0%
! GAGGAGGCTAGTGGCGGAGG+CGG - chr8.2:1497125-1497144 MS.gene68870:CDS 70.0%
GCGGAGAAGGAGCGACGCCG+TGG - chr8.2:1497150-1497169 MS.gene68870:CDS 75.0%
! GAGGCTAGTGGCGGAGGCGG+AGG - chr8.2:1497128-1497147 MS.gene68870:CDS 75.0%
! GCTAGTGGCGGAGGCGGAGG+CGG - chr8.2:1497131-1497150 MS.gene68870:CDS 75.0%
! GGCGGAGGCGGAGGCGGAGA+AGG - chr8.2:1497137-1497156 MS.gene68870:CDS 80.0%
!!! CCTTGCGGCGGCGTTGGCGG+CGG - chr8.2:1497082-1497101 MS.gene68870:CDS 80.0%
! CCGCCGCCAACGCCGCCGCA+AGG + chr8.2:1497085-1497104 None:intergenic 85.0%
!!! CGGCGGCGTTGGCGGCGGAG+CGG - chr8.2:1497087-1497106 MS.gene68870:CDS 85.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 1496726 1497412 1496726 ID=MS.gene68870
chr8.2 mRNA 1496726 1497412 1496726 ID=MS.gene68870.t1;Parent=MS.gene68870
chr8.2 exon 1496726 1497412 1496726 ID=MS.gene68870.t1.exon1;Parent=MS.gene68870.t1
chr8.2 CDS 1496726 1497412 1496726 ID=cds.MS.gene68870.t1;Parent=MS.gene68870.t1
Gene Sequence

>MS.gene68870

ATGAGTCAACTCGGTCTCATATTGCAGGAATCTCTGCGTTCTCAGAGAGAATCAAGAACCATTCTCGGTTTATTAACCGAACAAATGGACAGTTTAGATTCAGGAACCACCAGAAGACGTAGTCTCAAAGAACGCTTCAGATTCACCGGTATGGGTTGTTGTGGTGCCACCTGGGTTTTCCGTCCAATTATCCGCAACGAACATCATCATCAACAACAACAACAACAGCAAAACGAAGAAGGTGGAGCTACAGTGGAGCAACAACAACAACAGCTACAAGTTCAAGATCAAGATCCGAACCCAGTTGAACCGGAATGTGAAACTCCGGTGGTGGGTTCGTCTGGTTCTGGAATGAACCTTGCGGCGGCGTTGGCGGCGGAGCGGCAAATGAGGGTAACGGAGGAGGCTAGTGGCGGAGGCGGAGGCGGAGAAGGAGCGACGCCGTGGAGAGTTTCGTTGATGAAACTGTTGGAGGAAACGGAAGTGGAAAATGAGAGTAATGCTGGTGCGGTGGTTGTGGAAGGGAGTGATTCAGTGTGCTGCGTGTGCATGGGAAGAAAGAAAGGTGCAGCGTTCATACCGTGTGGACACACTTTTTGCAGAGTGTGTTCGAGGGAACTGTGGTTGAATCGAGGAAATTGTCCCCTTTGTAATCGTTCTATTCTCGAGATTCTCGACATTTTCTGA

Protein sequence

>MS.gene68870.t1

MSQLGLILQESLRSQRESRTILGLLTEQMDSLDSGTTRRRSLKERFRFTGMGCCGATWVFRPIIRNEHHHQQQQQQQNEEGGATVEQQQQQLQVQDQDPNPVEPECETPVVGSSGSGMNLAAALAAERQMRVTEEASGGGGGGEGATPWRVSLMKLLEETEVENESNAGAVVVEGSDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGNCPLCNRSILEILDIF