AlfalfaGEDB | MS.gene69207

M. sativa cultivar XinJiangDaYe / MS.gene69207

KEGG: -
GO: -
PFAM: PF03634.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69207.t1	KEH32038.1	95.7	139	5	1	1	138	23	161	2.60E-64	254.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69207.t1	Q9FME3	77.9	95	20	1	10	103	38	132	1.4e-37	157.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69207.t1	A0A072USI6	95.7	139	5	1	1	138	23	161	1.9e-64	254.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene69207.t1	TF	TCP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050128	MS.gene69207	0.825579	4.34E-54	-1.69E-46
MS.gene05316	MS.gene69207	0.808655	2.78E-50	-1.69E-46
MS.gene060495	MS.gene69207	0.809971	1.45E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69207.t1	MTR_4g109660	95.683	139	5	1	1	138	23	161	4.97e-82	243
MS.gene69207.t1	MTR_3g026050	76.147	109	24	1	4	110	25	133	1.40e-53	173
MS.gene69207.t1	MTR_7g015010	81.250	80	15	0	16	95	39	118	1.12e-44	149
MS.gene69207.t1	MTR_6g015350	64.706	102	32	2	16	116	31	129	4.29e-39	134
MS.gene69207.t1	MTR_4g079580	54.321	81	37	0	27	107	15	95	2.96e-23	92.8
MS.gene69207.t1	MTR_8g463380	52.809	89	42	0	7	95	27	115	2.99e-23	94.0
MS.gene69207.t1	MTR_2g090960	57.692	78	29	2	27	100	17	94	2.04e-22	90.5
MS.gene69207.t1	MTR_2g078200	57.971	69	29	0	27	95	13	81	1.47e-21	87.8
MS.gene69207.t1	MTR_1g103380	50.000	60	30	0	33	92	200	259	2.01e-13	66.2
MS.gene69207.t1	MTR_7g018500	49.123	57	29	0	36	92	122	178	4.14e-13	65.1
MS.gene69207.t1	MTR_6g017055	48.276	58	30	0	35	92	127	184	8.47e-13	64.3
MS.gene69207.t1	MTR_4g111935	49.091	55	28	0	35	89	133	187	2.20e-12	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69207.t1	AT5G60970	80.682	88	16	1	17	103	45	132	3.87e-48	158
MS.gene69207.t1	AT5G08070	75.610	82	20	0	22	103	21	102	1.80e-41	138
MS.gene69207.t1	AT5G08070	75.610	82	20	0	22	103	21	102	1.80e-41	138
MS.gene69207.t1	AT3G02150	64.078	103	30	3	17	112	57	159	3.86e-41	138
MS.gene69207.t1	AT3G02150	64.078	103	30	3	17	112	57	159	9.62e-41	139
MS.gene69207.t1	AT1G30210	62.105	95	31	2	4	96	21	112	8.57e-32	115
MS.gene69207.t1	AT1G30210	62.105	95	31	2	4	96	21	112	8.57e-32	115
MS.gene69207.t1	AT4G18390	68.493	73	23	0	24	96	32	104	9.63e-30	110
MS.gene69207.t1	AT4G18390	68.493	73	23	0	24	96	32	104	9.63e-30	110
MS.gene69207.t1	AT1G53230	61.644	73	25	1	27	96	42	114	1.43e-24	97.1
MS.gene69207.t1	AT1G53230	61.644	73	25	1	27	96	42	114	1.43e-24	97.1
MS.gene69207.t1	AT3G15030	62.121	66	25	0	27	92	38	103	3.47e-23	93.6
MS.gene69207.t1	AT3G15030	62.121	66	25	0	27	92	38	103	3.47e-23	93.6
MS.gene69207.t1	AT3G15030	62.121	66	25	0	27	92	38	103	3.47e-23	93.6
MS.gene69207.t1	AT3G15030	62.121	66	25	0	27	92	38	103	3.47e-23	93.6
MS.gene69207.t1	AT2G31070	60.606	66	26	0	27	92	22	87	1.92e-21	88.2
MS.gene69207.t1	AT1G68800	46.269	67	36	0	26	92	104	170	2.70e-15	71.2
MS.gene69207.t1	AT1G68800	46.269	67	36	0	26	92	104	170	2.79e-15	71.2
MS.gene69207.t1	AT1G67260	43.284	67	38	0	23	89	76	142	4.52e-14	67.8
MS.gene69207.t1	AT1G67260	43.284	67	38	0	23	89	76	142	4.52e-14	67.8
MS.gene69207.t1	AT1G67260	43.284	67	38	0	23	89	76	142	4.97e-14	67.8
MS.gene69207.t1	AT3G18550	39.024	82	49	1	11	92	126	206	1.64e-12	63.5
MS.gene69207.t1	AT3G18550	39.024	82	49	1	11	92	126	206	2.38e-12	63.2
MS.gene69207.t1	AT3G18550	39.024	82	49	1	11	92	126	206	2.41e-12	63.2
MS.gene69207.t1	AT3G18550	39.024	82	49	1	11	92	126	206	2.41e-12	63.2

Find 22 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACTACCTGGTTGCTGAAAT+AGG	0.245083	8.3:-15078830	None:intergenic
CGTGACACTCTCACTATTCT+TGG	0.252002	8.3:-15078579	None:intergenic
AGCAAAGTTTGCACAATTAG+AGG	0.305586	8.3:+15078627	MS.gene69207:CDS
TGATGTAGCTATTGCTTTGT+TGG	0.307657	8.3:-15078532	None:intergenic
GTGAGAGTGTCACGTTCTTT+AGG	0.317533	8.3:+15078588	MS.gene69207:CDS
GATTAAGAGACCGTAGGATT+AGG	0.348209	8.3:+15078649	MS.gene69207:CDS
ACTACCTGGTTGCTGAAATA+GGG	0.363637	8.3:-15078829	None:intergenic
AGAAGCTGAAAGTTCAGAAA+AGG	0.433467	8.3:+15078905	MS.gene69207:CDS
AACCTAGCAAAGTTGTTGAA+TGG	0.462940	8.3:+15078730	MS.gene69207:CDS
TATGGTATGAATCAAACTAA+AGG	0.480245	8.3:+15078879	MS.gene69207:CDS
TGTTGTTGAGTGAAACTTTG+AGG	0.510713	8.3:-15078804	None:intergenic
AACCATTCAACAACTTTGCT+AGG	0.525009	8.3:-15078732	None:intergenic
GAAGCTGAAAGTTCAGAAAA+GGG	0.530447	8.3:+15078906	MS.gene69207:CDS
TATGATCTTCAAGACAGACT+TGG	0.562288	8.3:+15078699	MS.gene69207:CDS
AGAGTGTCACGTTCTTTAGG+AGG	0.573587	8.3:+15078591	MS.gene69207:CDS
TCATATAGCTGAATTGCTGT+TGG	0.578184	8.3:-15078681	None:intergenic
GTACAGACAACCTAATCCTA+CGG	0.604529	8.3:-15078659	None:intergenic
AAACTTTGAGGAATATCAAG+TGG	0.636206	8.3:-15078792	None:intergenic
CAATAGCTACATCAAGACAA+TGG	0.639730	8.3:+15078541	MS.gene69207:CDS
CTTTGAGGAATATCAAGTGG+TGG	0.640320	8.3:-15078789	None:intergenic
CAAACCCTATTTCAGCAACC+AGG	0.642234	8.3:+15078825	MS.gene69207:CDS
TTAGAGGATTAAGAGACCGT+AGG	0.643012	8.3:+15078643	MS.gene69207:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATGGTATGAATCAAACTAA+AGG	+	chr8.3:15078879-15078898	MS.gene69207:CDS	25.0%
!	AAACTTTGAGGAATATCAAG+TGG	-	chr8.3:15078795-15078814	None:intergenic	30.0%
	AACCATTCAACAACTTTGCT+AGG	-	chr8.3:15078735-15078754	None:intergenic	35.0%
	AACCTAGCAAAGTTGTTGAA+TGG	+	chr8.3:15078730-15078749	MS.gene69207:CDS	35.0%
	AAGAGAAAAACAGTACTACC+TGG	-	chr8.3:15078846-15078865	None:intergenic	35.0%
	AGAAGCTGAAAGTTCAGAAA+AGG	+	chr8.3:15078905-15078924	MS.gene69207:CDS	35.0%
	AGCAAAGTTTGCACAATTAG+AGG	+	chr8.3:15078627-15078646	MS.gene69207:CDS	35.0%
	CAATAGCTACATCAAGACAA+TGG	+	chr8.3:15078541-15078560	MS.gene69207:CDS	35.0%
	TATGATCTTCAAGACAGACT+TGG	+	chr8.3:15078699-15078718	MS.gene69207:CDS	35.0%
	TCATATAGCTGAATTGCTGT+TGG	-	chr8.3:15078684-15078703	None:intergenic	35.0%
	TGATGTAGCTATTGCTTTGT+TGG	-	chr8.3:15078535-15078554	None:intergenic	35.0%
	TGTTGTTGAGTGAAACTTTG+AGG	-	chr8.3:15078807-15078826	None:intergenic	35.0%
!!	AGGTAGTACTGTTTTTCTCT+TGG	+	chr8.3:15078845-15078864	MS.gene69207:CDS	35.0%
!!	TAGTACTGTTTTTCTCTTGG+AGG	+	chr8.3:15078848-15078867	MS.gene69207:CDS	35.0%
	ACTACCTGGTTGCTGAAATA+GGG	-	chr8.3:15078832-15078851	None:intergenic	40.0%
	GATTAAGAGACCGTAGGATT+AGG	+	chr8.3:15078649-15078668	MS.gene69207:CDS	40.0%
	GTACAGACAACCTAATCCTA+CGG	-	chr8.3:15078662-15078681	None:intergenic	40.0%
	TACTACCTGGTTGCTGAAAT+AGG	-	chr8.3:15078833-15078852	None:intergenic	40.0%
	TTAGAGGATTAAGAGACCGT+AGG	+	chr8.3:15078643-15078662	MS.gene69207:CDS	40.0%
!	CTCTTGGAGGTGGTATTTTA+TGG	+	chr8.3:15078861-15078880	MS.gene69207:CDS	40.0%
!	CTTTGAGGAATATCAAGTGG+TGG	-	chr8.3:15078792-15078811	None:intergenic	40.0%
!!	TACTGTTTTTCTCTTGGAGG+TGG	+	chr8.3:15078851-15078870	MS.gene69207:CDS	40.0%
	CAAACCCTATTTCAGCAACC+AGG	+	chr8.3:15078825-15078844	MS.gene69207:CDS	45.0%
	CGTGACACTCTCACTATTCT+TGG	-	chr8.3:15078582-15078601	None:intergenic	45.0%
!	AGAGTGTCACGTTCTTTAGG+AGG	+	chr8.3:15078591-15078610	MS.gene69207:CDS	45.0%
!!	GTGAGAGTGTCACGTTCTTT+AGG	+	chr8.3:15078588-15078607	MS.gene69207:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	15078513	15078927	15078513	ID=MS.gene69207
chr8.3	mRNA	15078513	15078927	15078513	ID=MS.gene69207.t1;Parent=MS.gene69207
chr8.3	exon	15078513	15078927	15078513	ID=MS.gene69207.t1.exon1;Parent=MS.gene69207.t1
chr8.3	CDS	15078513	15078927	15078513	ID=cds.MS.gene69207.t1;Parent=MS.gene69207.t1

Gene Sequence

>MS.gene69207

ATGGAATCAGAGAAATTCTCCAACAAAGCAATAGCTACATCAAGACAATGGACATCATTAAGAAATCCAAGAATAGTGAGAGTGTCACGTTCTTTAGGAGGAAAAGACAGACACAGCAAAGTTTGCACAATTAGAGGATTAAGAGACCGTAGGATTAGGTTGTCTGTACCAACAGCAATTCAGCTATATGATCTTCAAGACAGACTTGGTTTAAGTCAACCTAGCAAAGTTGTTGAATGGTTAATTGAAGCAACTCAATCTGATATTGATAAATTACCACCACTTGATATTCCTCAAAGTTTCACTCAACAACAAACCCTATTTCAGCAACCAGGTAGTACTGTTTTTCTCTTGGAGGTGGTATTTTATGGTATGAATCAAACTAAAGGTAAAGAAGCTGAAAGTTCAGAAAAGG

Protein sequence

>MS.gene69207.t1

MESEKFSNKAIATSRQWTSLRNPRIVRVSRSLGGKDRHSKVCTIRGLRDRRIRLSVPTAIQLYDLQDRLGLSQPSKVVEWLIEATQSDIDKLPPLDIPQSFTQQQTLFQQPGSTVFLLEVVFYGMNQTKGKEAESSEK