Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69375.t1 | AES74062.2 | 90.2 | 368 | 35 | 1 | 9 | 376 | 11 | 377 | 5.70E-178 | 633.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69375.t1 | Q6DYE5 | 39.6 | 364 | 199 | 7 | 25 | 373 | 29 | 386 | 5.0e-58 | 226.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69375.t1 | G7J5U4 | 90.2 | 368 | 35 | 1 | 9 | 376 | 11 | 377 | 4.1e-178 | 633.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene69375 | 0.800183 | 1.62E-48 | -1.69E-46 |
| MS.gene049499 | MS.gene69375 | 0.807911 | 4.01E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene69375 | 0.800829 | 1.20E-48 | -1.69E-46 |
| MS.gene057155 | MS.gene69375 | 0.801282 | 9.69E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene69375 | 0.805368 | 1.38E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69375.t1 | MTR_3g114460 | 91.622 | 370 | 30 | 1 | 7 | 376 | 9 | 377 | 0.0 | 661 |
| MS.gene69375.t1 | MTR_8g068520 | 51.862 | 376 | 153 | 9 | 13 | 375 | 17 | 377 | 4.10e-120 | 353 |
| MS.gene69375.t1 | MTR_1g025960 | 51.596 | 376 | 151 | 8 | 11 | 375 | 11 | 366 | 1.45e-116 | 343 |
| MS.gene69375.t1 | MTR_1g100200 | 28.090 | 356 | 215 | 8 | 30 | 372 | 10 | 337 | 3.90e-29 | 115 |
| MS.gene69375.t1 | MTR_1g100200 | 28.169 | 355 | 214 | 8 | 31 | 372 | 14 | 340 | 5.07e-29 | 115 |
| MS.gene69375.t1 | MTR_1g100200 | 28.169 | 355 | 214 | 8 | 31 | 372 | 34 | 360 | 8.69e-29 | 115 |
| MS.gene69375.t1 | MTR_7g084240 | 24.211 | 285 | 180 | 12 | 97 | 376 | 124 | 377 | 7.22e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69375.t1 | AT1G20180 | 40.110 | 364 | 197 | 7 | 25 | 373 | 29 | 386 | 1.27e-69 | 224 |
| MS.gene69375.t1 | AT1G20180 | 36.813 | 364 | 178 | 8 | 25 | 373 | 29 | 355 | 1.32e-58 | 194 |
| MS.gene69375.t1 | AT3G49070 | 29.474 | 380 | 215 | 8 | 12 | 374 | 16 | 359 | 6.31e-35 | 132 |
| MS.gene69375.t1 | AT3G49070 | 29.737 | 380 | 214 | 8 | 12 | 374 | 56 | 399 | 1.39e-34 | 132 |
| MS.gene69375.t1 | AT3G19250 | 22.572 | 381 | 260 | 11 | 1 | 372 | 1 | 355 | 4.45e-11 | 64.3 |
Find 54 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATTGCCAAACTAACTTTA+TGG | 0.111324 | 3.3:+93484459 | MS.gene69375:CDS |
| TTTGCTTTCCATAGCATAAT+TGG | 0.142059 | 3.3:+93484145 | MS.gene69375:CDS |
| GCGGAAGTTAATAACATTAT+TGG | 0.198214 | 3.3:-93483969 | None:intergenic |
| CGTCAAACTAGATTTGATTA+TGG | 0.245353 | 3.3:+93483806 | MS.gene69375:CDS |
| AAGAGATCAAAGTGGGAAAA+TGG | 0.266547 | 3.3:+93484220 | MS.gene69375:CDS |
| TTATATAGTGATAAATGATT+TGG | 0.292938 | 3.3:+93484300 | MS.gene69375:CDS |
| GATATTTGTGTGAAGAATTA+TGG | 0.298600 | 3.3:+93484385 | MS.gene69375:CDS |
| TGTAACAAAGCTCAAGGATA+TGG | 0.306198 | 3.3:+93483593 | MS.gene69375:CDS |
| ACTGATCCATAAAGTTAGTT+TGG | 0.327126 | 3.3:-93484465 | None:intergenic |
| TATGTAGTTGGTGGATCCTT+TGG | 0.338019 | 3.3:+93484184 | MS.gene69375:CDS |
| GGTTAAGAGGTTAGAGGATG+AGG | 0.399784 | 3.3:+93484339 | MS.gene69375:CDS |
| GCTGCTGCCCCTTATGTAGT+TGG | 0.407925 | 3.3:+93484172 | MS.gene69375:CDS |
| TGCAAGAAAGTTGGAGGAAT+CGG | 0.409959 | 3.3:+93484079 | MS.gene69375:CDS |
| TTCTTTCTTCCGAAGAAGAT+CGG | 0.414371 | 3.3:+93483236 | MS.gene69375:CDS |
| TTTGTAACAATTTCTTGTCT+AGG | 0.414371 | 3.3:-93483686 | None:intergenic |
| TGTTGCGACAAAATCCTTCA+AGG | 0.417769 | 3.3:+93483779 | MS.gene69375:CDS |
| TTAACTCACCCGATCTTCTT+CGG | 0.418614 | 3.3:-93483245 | None:intergenic |
| CAAATTGATGTTGCTGCAAA+AGG | 0.419880 | 3.3:+93484274 | MS.gene69375:CDS |
| TTGCTTTCCATAGCATAATT+GGG | 0.423917 | 3.3:+93484146 | MS.gene69375:CDS |
| AAGAGGTTATGCAAGAAAGT+TGG | 0.425159 | 3.3:+93484070 | MS.gene69375:CDS |
| AAGGATCCACCAACTACATA+AGG | 0.429019 | 3.3:-93484181 | None:intergenic |
| AAGAATTATGGTCATGGTAA+TGG | 0.433359 | 3.3:+93484397 | MS.gene69375:CDS |
| TGAAGAAGAAGAAGTCGAAC+AGG | 0.439516 | 3.3:-93483654 | None:intergenic |
| TAGTTTGACGAACACCTTGA+AGG | 0.455617 | 3.3:-93483793 | None:intergenic |
| TCTTTCTTCCGAAGAAGATC+GGG | 0.456820 | 3.3:+93483237 | MS.gene69375:CDS |
| CCGTATGGTTAAGAGGTTAG+AGG | 0.460969 | 3.3:+93484333 | MS.gene69375:CDS |
| AACTAACTTTATGGATCAGT+TGG | 0.461239 | 3.3:+93484468 | MS.gene69375:CDS |
| CTATGAGCCGTATGGTTAAG+AGG | 0.474901 | 3.3:+93484326 | MS.gene69375:CDS |
| AAATTGATGTTGCTGCAAAA+GGG | 0.477002 | 3.3:+93484275 | MS.gene69375:CDS |
| AGGATCCACCAACTACATAA+GGG | 0.488584 | 3.3:-93484180 | None:intergenic |
| TAGAGGATGAGGTTGAACAT+TGG | 0.517451 | 3.3:+93484350 | MS.gene69375:CDS |
| GAAGAAGAAGAGATCAAAGT+GGG | 0.525471 | 3.3:+93484213 | MS.gene69375:CDS |
| TGAGGTTGAACATTGGAGAG+AGG | 0.530933 | 3.3:+93484357 | MS.gene69375:CDS |
| ACAAAGACATGCTTCTAAGC+TGG | 0.533545 | 3.3:-93483759 | None:intergenic |
| AGGTTATGCAAGAAAGTTGG+AGG | 0.533933 | 3.3:+93484073 | MS.gene69375:CDS |
| CTTCTTCTTCATCAAACCAA+AGG | 0.546523 | 3.3:-93484200 | None:intergenic |
| CAGCTAACCCAATTATGCTA+TGG | 0.556147 | 3.3:-93484153 | None:intergenic |
| GGATCAGTTGGAAGAGCTTG+AGG | 0.556162 | 3.3:+93484480 | MS.gene69375:CDS |
| AATTGATGTTGCTGCAAAAG+GGG | 0.557456 | 3.3:+93484276 | MS.gene69375:CDS |
| GCTGCCCCTTATGTAGTTGG+TGG | 0.559630 | 3.3:+93484175 | MS.gene69375:CDS |
| TATGACACTGACACAGATGT+AGG | 0.564280 | 3.3:+93483875 | MS.gene69375:CDS |
| TAAGGTGCATCATCTTCTAG+TGG | 0.565366 | 3.3:+93483724 | MS.gene69375:CDS |
| GACATATGTAACAAAGCTCA+AGG | 0.571855 | 3.3:+93483587 | MS.gene69375:CDS |
| CCTCTAACCTCTTAACCATA+CGG | 0.573081 | 3.3:-93484333 | None:intergenic |
| AATTTGTTCTGAATGCATTG+AGG | 0.584357 | 3.3:-93483556 | None:intergenic |
| TGAAGAAGAAGAGATCAAAG+TGG | 0.592386 | 3.3:+93484212 | MS.gene69375:CDS |
| TTTGGATACTATGAGCCGTA+TGG | 0.601069 | 3.3:+93484318 | MS.gene69375:CDS |
| ATTATGGTCATGGTAATGGA+AGG | 0.610111 | 3.3:+93484401 | MS.gene69375:CDS |
| AATATTGAAGATGGTTCTGA+GGG | 0.614569 | 3.3:+93484429 | MS.gene69375:CDS |
| AAGGTGTGTAATATTGAAGA+TGG | 0.621343 | 3.3:+93484420 | MS.gene69375:CDS |
| ATCATCGAAAGAACGTGATG+TGG | 0.634822 | 3.3:+93483493 | MS.gene69375:CDS |
| GGATCCACCAACTACATAAG+GGG | 0.639730 | 3.3:-93484179 | None:intergenic |
| TAATATTGAAGATGGTTCTG+AGG | 0.641414 | 3.3:+93484428 | MS.gene69375:CDS |
| TGTGTGAAGAATTATGGTCA+TGG | 0.650409 | 3.3:+93484391 | MS.gene69375:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACTAATTAATTAATTACT+TGG | - | chr3.3:93483401-93483420 | None:intergenic | 10.0% |
| !! | TTATATAGTGATAAATGATT+TGG | + | chr3.3:93484300-93484319 | MS.gene69375:CDS | 15.0% |
| !! | GAAGAAAAATAACAATAAAG+AGG | + | chr3.3:93484053-93484072 | MS.gene69375:CDS | 20.0% |
| !! | TTATTAATAAAAAAACCTGC+AGG | + | chr3.3:93483464-93483483 | MS.gene69375:intron | 20.0% |
| ! | AAAAATGATCAAGAGCTTTA+AGG | + | chr3.3:93483706-93483725 | MS.gene69375:CDS | 25.0% |
| ! | GATATTTGTGTGAAGAATTA+TGG | + | chr3.3:93484385-93484404 | MS.gene69375:CDS | 25.0% |
| ! | TTTGTAACAATTTCTTGTCT+AGG | - | chr3.3:93483689-93483708 | None:intergenic | 25.0% |
| !! | TGTCATATTCAACAACTTTT+TGG | - | chr3.3:93483862-93483881 | None:intergenic | 25.0% |
| AAATTGATGTTGCTGCAAAA+GGG | + | chr3.3:93484275-93484294 | MS.gene69375:CDS | 30.0% | |
| AACTAACTTTATGGATCAGT+TGG | + | chr3.3:93484468-93484487 | MS.gene69375:CDS | 30.0% | |
| AAGAATTATGGTCATGGTAA+TGG | + | chr3.3:93484397-93484416 | MS.gene69375:CDS | 30.0% | |
| AAGGTGTGTAATATTGAAGA+TGG | + | chr3.3:93484420-93484439 | MS.gene69375:CDS | 30.0% | |
| ACTGATCCATAAAGTTAGTT+TGG | - | chr3.3:93484468-93484487 | None:intergenic | 30.0% | |
| CGTCAAACTAGATTTGATTA+TGG | + | chr3.3:93483806-93483825 | MS.gene69375:CDS | 30.0% | |
| GCGGAAGTTAATAACATTAT+TGG | - | chr3.3:93483972-93483991 | None:intergenic | 30.0% | |
| TGATTGCCAAACTAACTTTA+TGG | + | chr3.3:93484459-93484478 | MS.gene69375:CDS | 30.0% | |
| TTAATAAAAAAACCTGCAGG+TGG | + | chr3.3:93483467-93483486 | MS.gene69375:intron | 30.0% | |
| TTGCTTTCCATAGCATAATT+GGG | + | chr3.3:93484146-93484165 | MS.gene69375:CDS | 30.0% | |
| TTTGCTTTCCATAGCATAAT+TGG | + | chr3.3:93484145-93484164 | MS.gene69375:CDS | 30.0% | |
| ! | AATTTGTTCTGAATGCATTG+AGG | - | chr3.3:93483559-93483578 | None:intergenic | 30.0% |
| ! | TAATATTGAAGATGGTTCTG+AGG | + | chr3.3:93484428-93484447 | MS.gene69375:CDS | 30.0% |
| ! | TGTGTTTTTCTTGAATATCG+CGG | - | chr3.3:93483991-93484010 | None:intergenic | 30.0% |
| !! | AATATTGAAGATGGTTCTGA+GGG | + | chr3.3:93484429-93484448 | MS.gene69375:CDS | 30.0% |
| AAGAGATCAAAGTGGGAAAA+TGG | + | chr3.3:93484220-93484239 | MS.gene69375:CDS | 35.0% | |
| AAGAGGTTATGCAAGAAAGT+TGG | + | chr3.3:93484070-93484089 | MS.gene69375:CDS | 35.0% | |
| AATTGATGTTGCTGCAAAAG+GGG | + | chr3.3:93484276-93484295 | MS.gene69375:CDS | 35.0% | |
| CAAATTGATGTTGCTGCAAA+AGG | + | chr3.3:93484274-93484293 | MS.gene69375:CDS | 35.0% | |
| CTTCTTCTTCATCAAACCAA+AGG | - | chr3.3:93484203-93484222 | None:intergenic | 35.0% | |
| GAAGAAGAAGAGATCAAAGT+GGG | + | chr3.3:93484213-93484232 | MS.gene69375:CDS | 35.0% | |
| GAATTGAACTCCAATATCAG+AGG | + | chr3.3:93483361-93483380 | MS.gene69375:intron | 35.0% | |
| GACATATGTAACAAAGCTCA+AGG | + | chr3.3:93483587-93483606 | MS.gene69375:CDS | 35.0% | |
| TAACAAAGCCACCAAAAAAG+CGG | - | chr3.3:93484122-93484141 | None:intergenic | 35.0% | |
| TGAAGAAGAAGAGATCAAAG+TGG | + | chr3.3:93484212-93484231 | MS.gene69375:CDS | 35.0% | |
| TGTAACAAAGCTCAAGGATA+TGG | + | chr3.3:93483593-93483612 | MS.gene69375:CDS | 35.0% | |
| TGTGTGAAGAATTATGGTCA+TGG | + | chr3.3:93484391-93484410 | MS.gene69375:CDS | 35.0% | |
| TTCTTTCTTCCGAAGAAGAT+CGG | + | chr3.3:93483236-93483255 | MS.gene69375:CDS | 35.0% | |
| !! | ATTATGGTCATGGTAATGGA+AGG | + | chr3.3:93484401-93484420 | MS.gene69375:CDS | 35.0% |
| AAGGATCCACCAACTACATA+AGG | - | chr3.3:93484184-93484203 | None:intergenic | 40.0% | |
| ACAAAGACATGCTTCTAAGC+TGG | - | chr3.3:93483762-93483781 | None:intergenic | 40.0% | |
| AGGATCCACCAACTACATAA+GGG | - | chr3.3:93484183-93484202 | None:intergenic | 40.0% | |
| AGGTTATGCAAGAAAGTTGG+AGG | + | chr3.3:93484073-93484092 | MS.gene69375:CDS | 40.0% | |
| ATAAAAAAACCTGCAGGTGG+TGG | + | chr3.3:93483470-93483489 | MS.gene69375:intron | 40.0% | |
| ATCATCGAAAGAACGTGATG+TGG | + | chr3.3:93483493-93483512 | MS.gene69375:CDS | 40.0% | |
| CAGCTAACCCAATTATGCTA+TGG | - | chr3.3:93484156-93484175 | None:intergenic | 40.0% | |
| CCTCTAACCTCTTAACCATA+CGG | - | chr3.3:93484336-93484355 | None:intergenic | 40.0% | |
| TAAGGTGCATCATCTTCTAG+TGG | + | chr3.3:93483724-93483743 | MS.gene69375:CDS | 40.0% | |
| TAGAGGATGAGGTTGAACAT+TGG | + | chr3.3:93484350-93484369 | MS.gene69375:CDS | 40.0% | |
| TAGTTTGACGAACACCTTGA+AGG | - | chr3.3:93483796-93483815 | None:intergenic | 40.0% | |
| TATGACACTGACACAGATGT+AGG | + | chr3.3:93483875-93483894 | MS.gene69375:CDS | 40.0% | |
| TCTTTCTTCCGAAGAAGATC+GGG | + | chr3.3:93483237-93483256 | MS.gene69375:CDS | 40.0% | |
| TGAAGAAGAAGAAGTCGAAC+AGG | - | chr3.3:93483657-93483676 | None:intergenic | 40.0% | |
| TGCAAGAAAGTTGGAGGAAT+CGG | + | chr3.3:93484079-93484098 | MS.gene69375:CDS | 40.0% | |
| TGTTGCGACAAAATCCTTCA+AGG | + | chr3.3:93483779-93483798 | MS.gene69375:CDS | 40.0% | |
| TTAACTCACCCGATCTTCTT+CGG | - | chr3.3:93483248-93483267 | None:intergenic | 40.0% | |
| TTGAACTCCAATATCAGAGG+TGG | + | chr3.3:93483364-93483383 | MS.gene69375:intron | 40.0% | |
| TTTGGATACTATGAGCCGTA+TGG | + | chr3.3:93484318-93484337 | MS.gene69375:CDS | 40.0% | |
| !! | TATGTAGTTGGTGGATCCTT+TGG | + | chr3.3:93484184-93484203 | MS.gene69375:CDS | 40.0% |
| !!! | CGTTTCAGAAACCGCTTTTT+TGG | + | chr3.3:93484108-93484127 | MS.gene69375:CDS | 40.0% |
| !!! | TTCAGAAACCGCTTTTTTGG+TGG | + | chr3.3:93484111-93484130 | MS.gene69375:CDS | 40.0% |
| AAGCACACCACCTCTGATAT+TGG | - | chr3.3:93483374-93483393 | None:intergenic | 45.0% | |
| AATATCAGAGGTGGTGTGCT+TGG | + | chr3.3:93483373-93483392 | MS.gene69375:intron | 45.0% | |
| CCGTATGGTTAAGAGGTTAG+AGG | + | chr3.3:93484333-93484352 | MS.gene69375:CDS | 45.0% | |
| CTATGAGCCGTATGGTTAAG+AGG | + | chr3.3:93484326-93484345 | MS.gene69375:CDS | 45.0% | |
| GGATCCACCAACTACATAAG+GGG | - | chr3.3:93484182-93484201 | None:intergenic | 45.0% | |
| GGTTAAGAGGTTAGAGGATG+AGG | + | chr3.3:93484339-93484358 | MS.gene69375:CDS | 45.0% | |
| TGAGGTTGAACATTGGAGAG+AGG | + | chr3.3:93484357-93484376 | MS.gene69375:CDS | 45.0% | |
| GGATCAGTTGGAAGAGCTTG+AGG | + | chr3.3:93484480-93484499 | MS.gene69375:CDS | 50.0% | |
| GCTGCCCCTTATGTAGTTGG+TGG | + | chr3.3:93484175-93484194 | MS.gene69375:CDS | 55.0% | |
| GCTGCTGCCCCTTATGTAGT+TGG | + | chr3.3:93484172-93484191 | MS.gene69375:CDS | 55.0% | |
| TTCGATGATCCACCACCTGC+AGG | - | chr3.3:93483482-93483501 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 93483222 | 93484582 | 93483222 | ID=MS.gene69375 |
| chr3.3 | mRNA | 93483222 | 93484582 | 93483222 | ID=MS.gene69375.t1;Parent=MS.gene69375 |
| chr3.3 | exon | 93483222 | 93483258 | 93483222 | ID=MS.gene69375.t1.exon1;Parent=MS.gene69375.t1 |
| chr3.3 | CDS | 93483222 | 93483258 | 93483222 | ID=cds.MS.gene69375.t1;Parent=MS.gene69375.t1 |
| chr3.3 | exon | 93483486 | 93484582 | 93483486 | ID=MS.gene69375.t1.exon2;Parent=MS.gene69375.t1 |
| chr3.3 | CDS | 93483486 | 93484582 | 93483486 | ID=cds.MS.gene69375.t1;Parent=MS.gene69375.t1 |
| Gene Sequence |
| Protein sequence |
