AlfalfaGEDB | MS.gene69395

M. sativa cultivar XinJiangDaYe / MS.gene69395

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0003824 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010333 GO:0010468 GO:0010556 GO:0016829 GO:0016835 GO:0016838 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00847.23 PF01397.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69395.t1	XP_003608379.2	76.2	160	37	1	197	356	50	208	2.20E-59	240.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69395.t1	Q8LDC8	43.4	136	52	1	400	510	73	208	1.2e-18	96.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69395.t1	G7JV28	76.2	160	37	1	197	356	50	208	1.6e-59	240.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene69395.t1	TF	AP2/ERF-ERF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057026	MS.gene69395	0.821146	4.71E-53	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69395.t1	MTR_4g093270	76.250	160	37	1	197	356	50	208	2.94e-81	258
MS.gene69395.t1	MTR_4g093510	59.884	172	57	1	187	346	31	202	8.17e-61	210
MS.gene69395.t1	MTR_4g093260	59.302	172	56	2	187	346	22	191	2.34e-59	209
MS.gene69395.t1	MTR_4g093260	59.302	172	56	2	187	346	31	200	3.03e-59	209
MS.gene69395.t1	MTR_1g070000	67.114	149	23	2	401	523	76	224	4.21e-56	189
MS.gene69395.t1	MTR_1g070015	70.504	139	16	1	402	515	77	215	6.24e-55	186
MS.gene69395.t1	MTR_1g069985	57.988	169	44	3	381	523	48	215	1.18e-51	177
MS.gene69395.t1	MTR_4g093280	53.488	172	68	1	187	346	31	202	1.06e-49	179
MS.gene69395.t1	MTR_3g086150	60.759	158	57	1	48	200	1067	1224	3.34e-48	182
MS.gene69395.t1	MTR_3g086150	67.073	82	27	0	25	106	988	1069	6.37e-23	104

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69395.t1	AT3G23240	46.094	128	44	1	408	510	81	208	6.97e-25	103
MS.gene69395.t1	AT2G31230	69.091	55	17	0	403	457	80	134	8.38e-19	86.7
MS.gene69395.t1	AT5G43410	60.938	64	20	1	405	463	11	74	1.89e-18	82.4
MS.gene69395.t1	AT4G17500	71.429	49	14	0	409	457	148	196	2.05e-16	80.1
MS.gene69395.t1	AT5G47220	71.429	49	14	0	409	457	117	165	2.87e-16	79.3
MS.gene69395.t1	AT5G51190	60.714	56	22	0	402	457	65	120	5.29e-16	77.8
MS.gene69395.t1	AT5G47230	64.151	53	19	0	405	457	152	204	6.53e-16	79.3
MS.gene69395.t1	AT3G23230	57.407	54	23	0	404	457	15	68	1.38e-15	74.3
MS.gene69395.t1	AT4G18450	69.388	49	15	0	409	457	110	158	1.92e-15	77.8
MS.gene69395.t1	AT2G44840	65.306	49	17	0	409	457	92	140	2.23e-15	76.3

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Find 108 sgRNAs with CRISPR-Local

Find 327 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTGTAAATAATAAAGC+AGG	0.455975	3.3:-93267225	MS.gene69395:CDS
AACAAATCTCTAGAGCTCAT+TGG	0.483447	3.3:-93270278	MS.gene69395:CDS
AACCACAAATAGTCACATGG+AGG	0.755899	3.3:-93269179	MS.gene69395:CDS
AACCTCCATGTGACTATTTG+TGG	0.367739	3.3:+93269177	None:intergenic
AAGACGACACTCTATTTCTT+AGG	0.316688	3.3:-93270226	MS.gene69395:CDS
AAGGTGTAAATAATAAAGCA+GGG	0.624242	3.3:-93267224	MS.gene69395:CDS
AATCAAAAGTCTTCATATAT+AGG	0.378865	3.3:-93267523	MS.gene69395:CDS
AATGAGATCCACATTCTTCA+GGG	0.494140	3.3:+93270172	None:intergenic
ACACAACAAGAAGAGGAAGA+AGG	0.533196	3.3:-93267456	MS.gene69395:CDS
ACAGAAACTCATTGCTAGCA+AGG	0.589154	3.3:-93269064	MS.gene69395:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGGTTCTTTCCATATTTGA+GGG	+	chr3.3:93268658-93268677	None:intergenic	30.0%
ACCTGTAAAATGTAGAAATG+AGG	+	chr3.3:93271648-93271667	None:intergenic	30.0%
AGATTCAATATGATTGCAGA+GGG	-	chr3.3:93271035-93271054	MS.gene69395:intron	30.0%
ATGTGTAGAATTTCATACCT+TGG	-	chr3.3:93267115-93267134	MS.gene69395:CDS	30.0%
ATTTGAATTGCCGATGAATT+TGG	+	chr3.3:93268788-93268807	None:intergenic	30.0%
CTACTTTAGATTGAAGACTA+TGG	+	chr3.3:93267165-93267184	None:intergenic	30.0%
CTGATCATATGGTGATTAAT+CGG	+	chr3.3:93270348-93270367	None:intergenic	30.0%
CTTTATTATGCTATGTACGT+CGG	+	chr3.3:93270374-93270393	None:intergenic	30.0%
GAAAACAAAAACAACATGCA+TGG	+	chr3.3:93266486-93266505	None:intergenic	30.0%
GAAGTTTATCAGCGTATAAT+TGG	+	chr3.3:93271999-93272018	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	93266298	93272065	93266298	ID=MS.gene69395
chr3.3	mRNA	93266298	93272065	93266298	ID=MS.gene69395.t1;Parent=MS.gene69395
chr3.3	exon	93271927	93272065	93271927	ID=MS.gene69395.t1.exon1;Parent=MS.gene69395.t1
chr3.3	CDS	93271927	93272065	93271927	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1
chr3.3	exon	93270582	93270751	93270582	ID=MS.gene69395.t1.exon2;Parent=MS.gene69395.t1
chr3.3	CDS	93270582	93270751	93270582	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1
chr3.3	exon	93270106	93270396	93270106	ID=MS.gene69395.t1.exon3;Parent=MS.gene69395.t1
chr3.3	CDS	93270106	93270396	93270106	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1
chr3.3	exon	93269065	93269343	93269065	ID=MS.gene69395.t1.exon4;Parent=MS.gene69395.t1
chr3.3	CDS	93269065	93269343	93269065	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1
chr3.3	exon	93268850	93268921	93268850	ID=MS.gene69395.t1.exon5;Parent=MS.gene69395.t1
chr3.3	CDS	93268850	93268921	93268850	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1
chr3.3	exon	93268630	93268716	93268630	ID=MS.gene69395.t1.exon6;Parent=MS.gene69395.t1
chr3.3	CDS	93268630	93268716	93268630	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1
chr3.3	exon	93268210	93268307	93268210	ID=MS.gene69395.t1.exon7;Parent=MS.gene69395.t1
chr3.3	CDS	93268210	93268307	93268210	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1
chr3.3	exon	93268049	93268118	93268049	ID=MS.gene69395.t1.exon8;Parent=MS.gene69395.t1
chr3.3	CDS	93268049	93268118	93268049	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1
chr3.3	exon	93267383	93267548	93267383	ID=MS.gene69395.t1.exon9;Parent=MS.gene69395.t1
chr3.3	CDS	93267383	93267548	93267383	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1
chr3.3	exon	93267104	93267309	93267104	ID=MS.gene69395.t1.exon10;Parent=MS.gene69395.t1
chr3.3	CDS	93267104	93267309	93267104	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1
chr3.3	exon	93266298	93266489	93266298	ID=MS.gene69395.t1.exon11;Parent=MS.gene69395.t1
chr3.3	CDS	93266298	93266489	93266298	ID=cds.MS.gene69395.t1;Parent=MS.gene69395.t1

Gene Sequence

>MS.gene69395

ATGACCACCCATCGGAACGGCGACAACAACCGGCAACCGCCATCTCTCATTGACCAGAAAATCAATCTCAGTCAATCTCTCCCGAGAATCCATTCTTCTCCGTCACGACTGCGGAACACGGCGGCGCCGATGAGGAAGAGTAAGAGAGAGACGGCCAGATGACGAAGGTAATTTCCAATCTAAAGCCATGCATGTTGTTTTTGTTTTCGTTCTCCGGTCGGCAGTAAATATTGTCCCGTTTTGGCTAACAATCGTGCTGTTCTTGAGATGTCTTCTATGGATCCTCTATCTTCTTCTTCCGCTTCTTCTTCTGCCTTTCCAGATCAACAACCTCCTAATCTCAGGTATATTCACGCTCCTTGAATCTTTCTGGATGATGATCTCAGTTATTTTGTTTCGGTTTTGTAGTTGTTTTTCCTGGATTTTTTTTAATCGATGGTTATTTTAAAATTTTATGCTGTTGATTAGATACATTGATAGGAAAATTTGTGTTTTTTAGGGTTTTATTTTTATTTTGAGGTGGTTTTTGCACGTGGGTCGTTTTTGTTTGTGTTTTAGAATTGAATTGAATGGAGTGGTTCTAGGGTTTTGAAAGTGGTTTTGTTGAATTGAATTTGATTGTGGATTTGGGTTTTTTTTTTGTCGGTTTCTGGAATTTTTTCTAAAGGTTGAGTCTTTTGGGGAATTGAAATTGAAAGAAGGTACACTGTTGGATACAAGACTGAAATTTGAGTCAAGTTTTCTCCTAAATGGCTTGTGTGAAATTGGGTTCCAAAGCTGACTTTGTAATCTCAAGGGTTATTAATAATAAAAAAAAATGTGTAGAATTTCATACCTTGGTTTACTTTCATTTGTATGTATTCACCATAGTCTTCAATCTAAAGTAGATGAAAATTTATCCATTAGCTTCGCAAGTATTGGCAGATAATGATGCATTTGAAAAGAAGTAGAGCTAACTAAACTAACTACTAACTCTGCAGACACAGACACAGACAAGATAATGATGCATTATTTGAAAAGAAGTAGAGAGATTCATTTTTTGTTAATGAAAATGTTTTTTTTTTGTAGGAGCTGAACAAGATTGATGCTTATACACAGACAAGAGTGGATCCTCAAATAATTGTTGATAAATTCAAAGAACATCATATGGTATTAAATTCGTTGTTATGAATCTCTATCACTACTGATTTTGAATTGTGATTTGATTCTGACTGTTCTCTTTGCTACTTGGTTGAAAATTTTACATTTCTATTGTGCAGCCTCAACAGTGAAAGTTGAACTGCAGCAGACTTCCTCTGAGATATTGGAAAAAAGATATTTCTGTTCTTTCCGAGGAGAAAGAGCAGGCTCAAGTATCTAGAGTTGCTGCAGAGACAGAGTTAGAGAGAATTAGAGATGCAGCAGACAGGCAGACAAGAGAACTATCAGAGGCCAGCAGTACCATAAAGGATCTAGAATTTGAACTATCTCAGGTTGAAAGTAAGGTAAATTTATTGACCCAAAAAAATAATGCTGATCAAGTTGTCTAAGACTGAATTGGAAGATGGATTGAAGAAGCTGCAAGATGAAGCAGCAAATAATGCTAGCAAGTTGGTAGGTTCCTCTGAAACTATAAAATCACTGGAAGATGCATTGTTGAAGGCGCAAGATGATATTTCTACCCTGGAAGATGCAAATAAAATTGCCAAACAGGAGATATCTTCGCTCAGTTTTTAAGTTAAATTCATACATGGATGAGTTAGCTGGAAAGAATGGCAGCTTAGAGAACAAATCTCTAGAGCTCATTGGATTCCTTAATGATCTTCAAGTGCTTATGAAAGACGACACTCTATTTCTTAGGATAAAACAATGCTTTGAGAGAAAATGTGAGACCCTGAAGAATGTGGATCTCATTGTGAGCAAAGTAAGAAACCATATTTCCCTGTCCGCTAAGGATTCTAGAGGGCACCTTGAGATGGAGGTAACATTCTTATTATCCTTTTTTAGCTGTTGTTATTTTCTCAAGTTGTGTATTTGATACTGTTTTGATTTAATCTACATATATTTGCCATCTAAATATTTCGAACACTATGATATGTGTCCATAGCCCTCACTTTACTTCTGTTTTATTTATTCTAAAATGTATTCTTCATTCACTCACCACTGATATGCTTTTATGGATACATTGAGTGAAAAGAAAAACACCTTGAAAACACCTAATACAACATAGTAGTTTGTGACTACCCTTTTTTGGACAGGTGTGCTTCCATACAAGCAATAGAGACCAACTCTTGTGCAAAAATTGGATACTTCTTTCCTTAATTTACCTCTTTGAATATGCTTTTATGAACCTTCTTCTATGGTAGTTTTTTTGTCTTCCCTCAAATATGGAAAGAACCTTGGTATTACCACGGACACCGTGAATTTGGTGAAATCACGGACACCTGGTTTTGTTGAATCGTGATAGTTTAGCTTTTGCCAAAATTACAATGACGTACCTGGATTCTCCCAAATTCATCGGCAATTCAAATATGTATTAAGAAATTAAGAATGACCAAGTTCATTTTTTCACCAAGAATGAGTACCTGATTTGAACTTGAGATTAATCAATGCACTTTTAATGTGCCAAATTTTTAATTGAGATTATTAGGGCATGAAATTCCTTCTGGGATGTCTTTTTCCCCATAAGAAAAGATATGTGCAAATTGCACCTTTTTTGTGTTATTTTAAAACCAATATTAAGGTAGTTTTTAACTACTTCATTACCATGTCTGACATCAGGTTTCAGCTCTGGCCGTACATTAAGCTAATGATCATCTTATGGCTCATCATACCGGATTTTGGACGAACTTCTTATGTTTATAACTACCTTATTCGCTCCATGAAACCACAAATAGTCACATGGAGGTTAAATAGCTACTGGAGAGATTGTTTTGCTGAGAAAGATAACTTTTTACTGCATGCAGAGAGATATATGAAAGAGAATGGAACTGAAACCTTACAGAAACTCATTGCTAGCAAGGTAATTACAGCTAAAAATGTCAAAATACTATAACTTAAGAAGAATTATATAGGGAAGCATGTATGTGTTTATGATAACTCTATATCTGCATTGGCAATTGAAAATTTTAGCTAATTCTGTTTTTTTTTTTTTTAATATTGAAGAACACAATGTGTATGCCTGATGTAGAAGCGACAAAGGAAACCATAGGTACTAATAGTAAAGAAATATTTAAGGTAAAGTTTTTGGTGTTAAGTCAATTGTGACATTAATAGTTTTTGTTACAAGTTCTTGTATGAGTAGATTCTACCTGTCTTAGTCTCATGGTGACATTTAGAGTGCTTAATGAGGGAGCTTAATCCTGTGCAGAAAAATGGAGAAAGACTCCAAGTAAAATACAAAGACATCAAAGATTTGGAGGCTATTGAGAAAAAAGATTTTCCTGCAACGAAGCAAGTAAGGATTCATTCATATTCACTGCAAGTAGAAATCTCCGAAGGACAAATAATTTTTAGACTTGATTTCAGTCACAATATGTCAGATGATTTACTTGAAATATGTGTTTCTTTGTTGGCCAAGGTGAAAGAGTTGGACCTCTTTCATAAATTACAATTTCTACCTGCATACATAGCAGAAATTCTTTGAGGAAATTTTATTTGAATAATTGCAATCTTTTTCGAGAAATTAGAAGGATTCATCACGTGTAGGAGCATTCCCTCATAAGGCTGCACATCCTTGAAAGATTTAGACCTCACACTTCTTGCTGCATACACCAGAGATTTTAACTTCTTCAGATTCAGTGGAATCTGTAAGGATTCTTAATGACACAAATGCTCCAAGCCTAAGTGAAGAGGCTATGCAAGCTTTGCAAATAGTGTAAGACTTCATCTTCATTGATGCTTCAGTTCTATTGCAACCTGCTTAGGTTACCTCTCTAATTTTTCTGCAGATGATGATTACATATCAGGAGAAATGTGAACACTGATACAAGAGCCTTGATGGAGTTCAAAACTGGAGTAAGGAATAAAATGAATCAGGTATGATAATTGATTCTGAATTGCTGCAAGCCGATTAATCACCATATGATCAGCTTCCGACGTACATAGCATAATAAAGTATTTGTAAGCTTTCTTTCAACAATTTACTTTATTTATCTGCAAAGCTAAATCCAAGATACTTGCACAACGACTTTTATGTTTCATAATAATTATTTGACAATAAAAATGAGTTTTGTGAGTGTAAATAAGAATATTTGACTTTATAATTTGAAGTACTTGATCCAATTGTTGTTTTATTACTTTATAATTTGAAGTACTTGATCCGATTATTGTTTCTTGCTTCATTGTCAAAAATTCAGATGGTTAGCTGTTTGATTACATTGCTAAATATGCAATATTGTTTGTGTGGTTTTCTTTCCTTTGATATGATTGATTTCTCATCGAGTTCGCAGACAACAATTACCGAAAATGAGCCAAAACAATTGATGTTGAAGTCATCATCATCTGAAGCAAAGGAAGTGTTGCAGAATAATAATTAGATGAATCAAAAGTCTTCATATATAGGTGTGAGAAAAAGACCTAGGGGAAAATATGCAGCAGAGATCAGAGACACAACAAGAAGAGGAAGAAGGGTTTGGCTTGGAACATTTGATAGTGTTGAGGATGCTGCTTTGGCTTACGATCAAGCTGCATTTTCAATGAGAGGTAGTAATGTTGTGCTCAATTTTTCTGTTCAAAGGGTCAAAGGATCTTTGCAAGAGATTCAATATGATTGCAGAGGGGTTTTCACATGAACTTGCACTCAAGGAGAGACACTGTGAACAAAGAACGATGTTATCCAAAGGTGTAAATAATAAAGCAGGGAAACAAGATCAATCAGAGGGATTAAGTGTCTTGGTGTTAGAAGACTTGGGAGTTAAATATCTTGAGCAACTTCCAACAATATCTGATAAAGTACAAGCAGCAGCAACTTACGATCAAGTTGTATGTAATTTTTATTACTTCTTTTTAATTGATGCAGTAATTCTTTTTACTTGAACTAATATTATTACTTATGAAGAACTATCCATTGTAATTTTGGATCAATTGGTTGTGATTACTTTATGATAAAAACTTATATTTCAGAAAATTTGGTCGATTACAATAAATACTAACTATGTTCTGCTTTAGGAGCCATCGTCGAGAAATCATGTGAGTTAAAATTTGGTTGATTCACTCCTCTCTTTGGTGTGATTTAAAATTTACTTTGAAACAAAATCTTATCATAGATTGTCTTTCATTATATATGGTATTCTTCAAAGGTTTATTTAATTTTGGTTGGGGAAGATCTTTTCTATGTTTTCCTTACCTTCTGCAGTGCAACTAATTTCCTCATTTCTACATTTTACAGGTGAAACAATAATTTTGATACTCTTTGGATTATGTACAGCTCATATAGGATACTTTTGGTGGTTCTAACTTCTCTAAATATATAATAGGTAATAATGCTTAAGTTGTCTGCTATATTGTTGAGTTGATAAGTTTTACTCTAAACATTTATACAGTTGAGCTTATTTTGAGGTTTTAGTGTTTCTAACTTCTCTAAATATATTCTTCAGGATGTTAGTGCTGTTTTAGAGCTATATAAGGCTTCACAAGTCATTATACATCCTGATGAATCAATTTTGGTTAAACAAAGTTCTTGGACAAGACATTTTTTGGAGCAAGATTCTTCCCCTTACCAATTATACGCTGATAAACTTCAGATTTATGTTGATAACGAGGTAGTTTATTGTCTCAATCTTTCATAA

Protein sequence

>MS.gene69395.t1

MTTHRNGDNNRQPPSLIDQKINLSQSLPRIHSSPSRLRNTAAPMRKNISVLSEEKEQAQVSRVAAETELERIRDAADRQTRELSEASSTIKDLEFELSQVESKMQIKLPNRRYLRSVFKLNSYMDELAGKNGSLENKSLELIGFLNDLQVLMKDDTLFLRIKQCFERKCETLKNVDLIVSKVRNHISLSAKDSRGHLEMEVVFNYFITMSDIRFQLWPYIKLMIILWLIIPDFGRTSYVYNYLIRSMKPQIVTWRLNSYWRDCFAEKDNFLLHAERYMKENGTETLQKLIASKNTMCMPDVEATKETIGTNSKEIFKKNGERLQVKYKDIKDLEAIEKKDFPATKQILTSSDSVESVRILNDTNAPSLSEEAMQALQIVRNVNTDTRALMEFKTGVRNKMNQMNQKSSYIGVRKRPRGKYAAEIRDTTRRGRRVWLGTFDSVEDAALAYDQAAFSMREGFSHELALKERHCEQRTMLSKGVNNKAGKQDQSEGLSVLVLEDLGVKYLEQLPTISDKVQAAATYDQVDVSAVLELYKASQVIIHPDESILVKQSSWTRHFLEQDSSPYQLYADKLQIYVDNEVVYCLNLS