AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69507

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69507.t1 MTR_3g046300 93.318 449 28 1 56 504 15 461 0.0 841
MS.gene69507.t1 MTR_5g091130 69.665 567 98 12 1 504 1 556 0.0 733
MS.gene69507.t1 MTR_3g097150 37.471 435 230 13 79 504 48 449 1.47e-77 250
MS.gene69507.t1 MTR_1g111350 38.560 389 199 10 121 501 111 467 2.39e-75 245
MS.gene69507.t1 MTR_7g117430 39.175 388 200 9 123 501 124 484 5.06e-74 242
MS.gene69507.t1 MTR_1g115210 31.601 481 281 12 35 501 40 486 4.31e-71 234
MS.gene69507.t1 MTR_4g007740 33.249 394 239 10 112 501 104 477 3.23e-64 216
MS.gene69507.t1 MTR_1g039270 34.656 378 211 7 132 501 120 469 1.10e-62 212
MS.gene69507.t1 MTR_8g103880 31.017 403 242 8 107 502 83 456 2.38e-60 205
MS.gene69507.t1 MTR_2g028190 30.717 446 256 14 63 501 52 451 6.98e-60 204
MS.gene69507.t1 MTR_6g085010 36.188 362 205 8 140 501 156 491 3.51e-59 203
MS.gene69507.t1 MTR_3g053710 34.324 370 221 9 137 501 114 466 4.73e-59 202
MS.gene69507.t1 MTR_0036s0110 34.286 385 214 11 131 503 85 442 2.31e-58 199
MS.gene69507.t1 MTR_0036s0070 32.642 386 221 11 125 504 75 427 3.17e-57 196
MS.gene69507.t1 MTR_5g038360 33.516 364 225 9 141 503 89 436 8.06e-57 195
MS.gene69507.t1 MTR_8g096990 32.134 389 241 10 117 504 66 432 1.08e-55 192
MS.gene69507.t1 MTR_5g044770 32.292 384 239 9 122 504 68 431 3.18e-55 191
MS.gene69507.t1 MTR_3g053980 36.096 374 215 9 131 501 133 485 1.41e-54 191
MS.gene69507.t1 MTR_8g038280 33.423 371 223 11 139 504 85 436 2.32e-53 186
MS.gene69507.t1 MTR_5g080890 33.509 379 232 11 128 504 74 434 6.59e-53 185
MS.gene69507.t1 MTR_7g105850 31.841 402 210 14 139 501 89 465 5.54e-52 183
MS.gene69507.t1 MTR_2g059460 33.508 382 226 15 128 503 47 406 6.11e-52 182
MS.gene69507.t1 MTR_5g080960 33.245 379 233 11 128 504 74 434 2.05e-51 181
MS.gene69507.t1 MTR_5g008150 31.622 370 229 10 139 503 62 412 1.23e-47 170
MS.gene69507.t1 MTR_3g028120 31.551 374 229 12 138 504 86 439 4.11e-47 169
MS.gene69507.t1 MTR_6g090290 33.420 383 232 10 126 503 72 436 4.46e-47 169
MS.gene69507.t1 MTR_0036s0050 30.730 397 221 11 125 503 75 435 5.53e-47 169
MS.gene69507.t1 MTR_2g059490 31.429 385 235 13 129 504 47 411 4.10e-46 167
MS.gene69507.t1 MTR_5g008110 30.458 371 231 11 139 503 66 415 2.09e-45 164
MS.gene69507.t1 MTR_5g044570 31.720 372 207 10 135 503 84 411 6.17e-45 163
MS.gene69507.t1 MTR_8g038250 30.280 393 227 10 117 504 66 416 9.30e-45 162
MS.gene69507.t1 MTR_8g038310 30.370 405 243 10 100 503 47 413 1.26e-44 162
MS.gene69507.t1 MTR_1g037630 33.604 369 210 10 138 501 90 428 1.05e-43 160
MS.gene69507.t1 MTR_0024s0040 32.337 368 228 11 139 503 61 410 1.81e-43 159
MS.gene69507.t1 MTR_3g014290 33.333 369 211 10 138 501 78 416 5.90e-43 157
MS.gene69507.t1 MTR_2g067830 30.376 372 231 13 139 503 56 406 9.35e-43 157
MS.gene69507.t1 MTR_8g039540 33.154 371 212 11 138 502 94 434 8.36e-41 152
MS.gene69507.t1 MTR_1g060750 29.650 371 213 11 138 503 49 376 2.57e-40 149
MS.gene69507.t1 MTR_2g105300 30.084 359 207 16 155 498 50 379 2.44e-39 147
MS.gene69507.t1 MTR_4g045857 36.759 253 147 7 129 379 47 288 1.41e-38 142
MS.gene69507.t1 MTR_5g008070 29.551 379 227 13 134 501 75 424 9.50e-38 144
MS.gene69507.t1 MTR_4g056420 29.744 390 222 16 138 502 75 437 1.03e-37 144
MS.gene69507.t1 MTR_2g063110 29.398 415 240 13 129 502 107 509 2.40e-37 144
MS.gene69507.t1 MTR_2g084550 29.319 382 239 13 136 502 73 438 2.90e-37 143
MS.gene69507.t1 MTR_4g109760 32.578 353 213 12 155 504 69 399 1.66e-36 139
MS.gene69507.t1 MTR_4g100990 28.467 411 231 18 140 501 74 470 6.19e-35 137
MS.gene69507.t1 MTR_6g088760 31.454 337 202 14 138 465 86 402 8.97e-35 137
MS.gene69507.t1 MTR_7g062360 28.608 395 221 15 143 504 62 428 1.05e-33 132
MS.gene69507.t1 MTR_3g100500 30.709 381 231 10 128 502 85 438 1.58e-33 132
MS.gene69507.t1 MTR_7g102750 28.042 378 242 13 139 502 80 441 1.93e-33 133
MS.gene69507.t1 MTR_5g012490 26.506 415 233 15 140 501 74 469 1.62e-32 130
MS.gene69507.t1 MTR_2g055840 27.540 374 244 9 141 501 14 373 8.79e-32 126
MS.gene69507.t1 MTR_1g061940 27.202 386 245 13 135 502 78 445 4.76e-31 126
MS.gene69507.t1 MTR_3g068000 26.289 388 235 14 143 501 76 441 6.30e-31 125
MS.gene69507.t1 MTR_2g070430 27.720 386 234 12 138 498 85 450 6.72e-31 125
MS.gene69507.t1 MTR_4g095270 24.806 387 233 12 138 504 78 426 1.35e-30 125
MS.gene69507.t1 MTR_4g095270 24.806 387 233 12 138 504 78 426 4.02e-30 124
MS.gene69507.t1 MTR_3g065160 27.806 392 214 15 143 504 77 429 1.14e-29 121
MS.gene69507.t1 MTR_7g102750 29.358 327 203 12 139 453 80 390 1.18e-29 121
MS.gene69507.t1 MTR_1g078240 29.851 402 220 20 132 502 61 431 3.15e-29 120
MS.gene69507.t1 MTR_5g022880 28.941 387 211 17 141 501 113 461 4.49e-29 120
MS.gene69507.t1 MTR_5g077850 27.487 382 224 15 147 504 88 440 4.61e-29 119
MS.gene69507.t1 MTR_5g016260 24.802 379 243 12 138 504 86 434 1.65e-28 119
MS.gene69507.t1 MTR_7g080230 27.815 453 260 21 82 503 16 432 2.43e-28 118
MS.gene69507.t1 MTR_1g078240 29.851 402 219 21 132 502 61 430 3.36e-28 117
MS.gene69507.t1 MTR_5g016260 24.802 379 243 12 138 504 86 434 3.77e-28 118
MS.gene69507.t1 MTR_8g075090 29.016 386 214 16 141 503 113 461 5.28e-26 111
MS.gene69507.t1 MTR_1g078240 33.228 316 164 17 132 424 61 352 7.37e-26 109
MS.gene69507.t1 MTR_8g075090 28.682 387 216 16 141 504 113 462 2.06e-25 109
MS.gene69507.t1 MTR_7g021510 24.607 382 252 13 139 502 71 434 4.39e-25 108
MS.gene69507.t1 MTR_1g093100 25.635 394 222 14 149 499 55 420 1.55e-24 106
MS.gene69507.t1 MTR_5g061690 28.070 399 239 19 122 503 84 451 1.95e-24 107
MS.gene69507.t1 MTR_6g087710 60.976 82 27 1 428 504 1 82 2.79e-24 96.7
MS.gene69507.t1 MTR_1g072380 23.515 404 236 13 140 497 56 432 6.78e-24 104
MS.gene69507.t1 MTR_8g075010 24.786 468 269 19 76 504 29 452 2.14e-23 103
MS.gene69507.t1 MTR_3g087780 27.723 404 239 18 122 504 82 453 8.40e-23 102
MS.gene69507.t1 MTR_1g072450 25.258 388 228 17 149 499 55 417 2.77e-22 99.8
MS.gene69507.t1 MTR_1g072420 24.936 393 224 15 149 499 55 418 6.92e-22 98.6
MS.gene69507.t1 MTR_4g023650 24.634 410 247 15 124 504 46 422 7.04e-22 98.2
MS.gene69507.t1 MTR_8g075090 29.526 359 193 16 141 476 113 434 9.79e-22 98.6
MS.gene69507.t1 MTR_6g084710 26.768 396 236 17 132 501 183 550 5.03e-21 96.7
MS.gene69507.t1 MTR_3g096930 25.581 387 235 15 139 504 83 437 8.10e-21 95.5
MS.gene69507.t1 MTR_5g077920 26.396 394 213 14 147 503 75 428 1.11e-20 94.7
MS.gene69507.t1 MTR_4g023630 24.752 404 254 16 124 504 46 422 1.99e-20 94.0
MS.gene69507.t1 MTR_5g008090 27.365 296 195 10 213 503 34 314 2.05e-19 89.4
MS.gene69507.t1 MTR_2g022450 23.544 395 250 14 136 497 36 411 2.41e-19 90.9
MS.gene69507.t1 MTR_2g022490 25.128 390 221 15 141 488 44 404 3.48e-19 90.1
MS.gene69507.t1 MTR_1g029810 25.323 387 238 17 139 504 56 412 3.57e-19 90.1
MS.gene69507.t1 MTR_1g029810 25.323 387 238 17 139 504 56 412 8.60e-19 89.4
MS.gene69507.t1 MTR_2g022380 26.098 387 231 17 145 497 44 409 1.55e-18 88.6
MS.gene69507.t1 MTR_5g061690 26.510 298 189 14 213 503 38 312 9.58e-18 85.5
MS.gene69507.t1 MTR_6g084710 28.571 294 165 13 132 411 183 445 9.95e-18 86.3
MS.gene69507.t1 MTR_2g022350 26.031 388 230 18 145 497 44 409 1.25e-17 85.5
MS.gene69507.t1 MTR_2g022460 24.868 378 242 14 140 497 39 394 1.45e-17 85.1
MS.gene69507.t1 MTR_2g022430 24.691 405 227 17 141 499 40 412 2.42e-17 84.7
MS.gene69507.t1 MTR_2g022480 23.885 381 237 16 141 488 50 410 2.53e-16 81.6
MS.gene69507.t1 MTR_7g080230 27.901 362 199 19 172 503 33 362 2.73e-16 81.3
MS.gene69507.t1 MTR_7g113790 23.250 400 257 18 138 504 48 430 1.19e-15 79.3
MS.gene69507.t1 MTR_2g022370 24.403 377 246 14 145 497 44 405 2.20e-15 78.6
MS.gene69507.t1 MTR_4g117090 22.660 406 250 16 140 497 49 438 2.76e-15 78.6
MS.gene69507.t1 MTR_3g087780 25.852 352 217 15 167 504 26 347 6.55e-15 77.0
MS.gene69507.t1 MTR_3g087780 25.826 333 206 14 183 504 9 311 6.70e-15 77.0
MS.gene69507.t1 MTR_2g022320 23.214 392 243 11 141 497 37 405 9.92e-15 76.6
MS.gene69507.t1 MTR_0252s0030 25.532 188 121 5 293 465 144 327 8.00e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69507.t1 AT3G59080 67.342 444 131 7 65 504 102 535 0.0 597
MS.gene69507.t1 AT2G42980 65.101 447 133 8 65 504 97 527 0.0 559
MS.gene69507.t1 AT3G59080 61.712 444 120 7 65 504 102 499 0.0 526
MS.gene69507.t1 AT3G25700 40.731 383 206 12 132 501 75 449 5.81e-82 262
MS.gene69507.t1 AT1G01300 38.318 428 210 13 91 501 94 484 2.88e-75 245
MS.gene69507.t1 AT1G25510 35.745 470 237 11 39 501 72 483 1.71e-74 243
MS.gene69507.t1 AT3G61820 36.364 451 234 11 63 501 74 483 3.82e-74 242
MS.gene69507.t1 AT2G03200 33.259 448 241 13 79 504 50 461 5.37e-71 233
MS.gene69507.t1 AT3G18490 38.701 385 205 11 122 501 142 500 1.48e-68 228
MS.gene69507.t1 AT5G10770 36.410 390 221 11 116 502 109 474 1.76e-66 222
MS.gene69507.t1 AT3G20015 31.293 441 238 7 69 501 87 470 8.97e-62 209
MS.gene69507.t1 AT1G79720 31.519 441 273 10 69 501 63 482 1.49e-59 204
MS.gene69507.t1 AT5G33340 35.598 368 219 8 138 504 87 437 3.99e-56 194
MS.gene69507.t1 AT2G35615 34.439 392 234 12 122 502 65 444 2.88e-55 191
MS.gene69507.t1 AT3G12700 30.416 457 259 18 67 502 43 461 7.50e-53 186
MS.gene69507.t1 AT1G64830 33.867 375 228 10 131 504 76 431 3.12e-52 183
MS.gene69507.t1 AT1G31450 34.293 382 229 12 130 502 74 442 6.83e-52 182
MS.gene69507.t1 AT3G52500 30.568 458 252 20 82 502 40 468 9.45e-50 177
MS.gene69507.t1 AT3G54400 30.653 398 223 12 122 501 62 424 1.32e-48 173
MS.gene69507.t1 AT5G10760 33.684 380 220 11 123 501 116 464 6.18e-47 169
MS.gene69507.t1 AT2G28010 29.111 371 225 9 136 504 60 394 6.14e-45 162
MS.gene69507.t1 AT2G28040 29.275 386 231 9 122 504 38 384 1.13e-44 162
MS.gene69507.t1 AT2G28040 29.275 386 231 9 122 504 47 393 2.68e-44 161
MS.gene69507.t1 AT1G09750 31.169 385 225 12 130 502 93 449 5.07e-44 161
MS.gene69507.t1 AT4G30030 30.214 374 220 13 139 501 76 419 1.94e-42 156
MS.gene69507.t1 AT5G07030 32.361 377 210 13 138 502 112 455 1.55e-41 155
MS.gene69507.t1 AT4G30040 30.179 391 240 11 116 496 60 427 1.13e-40 152
MS.gene69507.t1 AT2G28030 28.219 365 226 8 141 504 61 390 6.20e-39 146
MS.gene69507.t1 AT2G28225 26.829 369 230 7 141 504 67 400 7.91e-36 138
MS.gene69507.t1 AT5G22850 28.682 387 235 15 136 502 76 441 8.77e-36 139
MS.gene69507.t1 AT2G28220 27.957 372 222 10 141 504 67 400 2.21e-35 136
MS.gene69507.t1 AT5G36260 27.604 384 233 16 139 502 76 434 8.00e-33 131
MS.gene69507.t1 AT2G36670 27.559 381 239 13 139 502 98 458 1.43e-32 130
MS.gene69507.t1 AT1G08210 28.205 390 230 15 136 502 113 475 3.34e-32 129
MS.gene69507.t1 AT1G08210 28.205 390 230 15 136 502 127 489 3.55e-32 130
MS.gene69507.t1 AT2G36670 27.441 379 238 13 141 502 34 392 3.92e-32 128
MS.gene69507.t1 AT2G36670 27.441 379 238 13 141 502 34 392 3.92e-32 128
MS.gene69507.t1 AT2G36670 27.441 379 238 13 141 502 105 463 4.07e-32 129
MS.gene69507.t1 AT1G08210 28.205 390 230 15 136 502 79 441 4.88e-32 129
MS.gene69507.t1 AT5G02190 26.823 384 228 14 150 502 82 443 5.49e-32 128
MS.gene69507.t1 AT4G12920 29.972 357 203 16 141 489 58 375 3.96e-31 124
MS.gene69507.t1 AT5G43100 26.509 381 237 14 136 504 71 420 5.41e-31 125
MS.gene69507.t1 AT2G23945 27.130 446 285 17 74 504 31 451 7.27e-31 125
MS.gene69507.t1 AT5G43100 26.509 381 237 14 136 504 71 420 2.18e-30 125
MS.gene69507.t1 AT5G45120 28.780 410 229 19 141 501 83 478 2.65e-30 124
MS.gene69507.t1 AT1G65240 25.654 382 240 13 139 502 72 427 7.12e-30 122
MS.gene69507.t1 AT4G33490 28.680 394 216 18 139 504 58 414 8.49e-30 121
MS.gene69507.t1 AT1G05840 26.728 434 252 19 104 502 37 439 1.65e-29 121
MS.gene69507.t1 AT2G39710 26.098 387 227 18 143 497 67 426 2.42e-29 120
MS.gene69507.t1 AT1G66180 26.633 398 216 17 142 504 73 429 4.38e-28 116
MS.gene69507.t1 AT5G37540 25.641 429 261 17 107 502 36 439 7.05e-28 116
MS.gene69507.t1 AT3G02740 25.393 382 243 15 139 502 83 440 7.34e-28 116
MS.gene69507.t1 AT1G77480 27.318 399 230 15 132 504 58 422 9.29e-28 115
MS.gene69507.t1 AT1G77480 27.089 395 236 14 132 504 58 422 1.37e-27 115
MS.gene69507.t1 AT3G02740 25.450 389 244 15 134 502 3 365 1.64e-27 114
MS.gene69507.t1 AT1G08210 27.098 417 227 17 136 502 79 468 2.65e-27 115
MS.gene69507.t1 AT3G50050 24.227 388 235 14 138 504 90 439 2.31e-25 110
MS.gene69507.t1 AT3G51350 26.494 385 229 15 141 503 102 454 3.60e-24 106
MS.gene69507.t1 AT2G17760 26.579 380 226 16 141 501 104 449 3.44e-23 103
MS.gene69507.t1 AT3G25700 40.876 137 75 4 132 266 75 207 5.02e-23 100
MS.gene69507.t1 AT3G25700 42.857 133 70 5 373 501 217 347 5.52e-23 100
MS.gene69507.t1 AT4G35880 26.650 394 213 16 141 504 107 454 9.67e-23 102
MS.gene69507.t1 AT1G03230 25.641 390 222 16 149 497 53 415 1.22e-22 100
MS.gene69507.t1 AT4G33490 28.693 352 189 17 139 465 55 369 1.58e-22 100
MS.gene69507.t1 AT1G44130 26.196 397 235 18 132 504 40 402 1.24e-21 97.4
MS.gene69507.t1 AT3G51360 25.984 381 232 15 141 503 5 353 9.94e-21 94.7
MS.gene69507.t1 AT3G51360 25.564 399 239 16 131 503 71 437 1.46e-20 95.1
MS.gene69507.t1 AT3G51360 25.564 399 239 16 131 503 81 447 1.48e-20 95.1
MS.gene69507.t1 AT5G10080 26.185 401 236 16 129 503 89 455 1.39e-19 92.0
MS.gene69507.t1 AT4G35880 27.368 380 194 17 141 484 107 440 2.98e-19 90.5
MS.gene69507.t1 AT3G51340 25.628 398 244 15 124 503 86 449 3.18e-19 90.9
MS.gene69507.t1 AT4G16563 26.034 411 218 17 158 501 100 491 1.49e-18 89.0
MS.gene69507.t1 AT1G49050 23.529 391 244 18 141 501 30 395 2.98e-18 87.4
MS.gene69507.t1 AT3G51330 25.131 382 239 13 141 503 102 455 3.33e-17 84.7
MS.gene69507.t1 AT3G51330 25.000 380 238 13 141 501 102 453 5.96e-17 83.6
MS.gene69507.t1 AT3G51340 24.205 409 248 14 124 503 86 461 9.88e-17 83.6
MS.gene69507.t1 AT3G51360 25.570 395 228 18 131 492 81 442 1.39e-15 79.3
MS.gene69507.t1 AT3G51360 25.995 377 221 17 141 492 89 432 1.41e-15 79.3
MS.gene69507.t1 AT3G51340 25.868 317 189 11 124 423 86 373 1.56e-15 79.0
MS.gene69507.t1 AT3G51340 25.633 316 189 11 124 422 86 372 8.59e-15 76.6
MS.gene69507.t1 AT3G42550 37.755 98 57 1 138 235 75 168 8.84e-14 73.6
MS.gene69507.t1 AT3G51360 24.545 330 208 13 183 503 14 311 8.98e-14 73.2
MS.gene69507.t1 AT3G51360 24.545 330 208 13 183 503 14 311 8.98e-14 73.2
MS.gene69507.t1 AT3G51330 25.281 356 219 13 141 477 102 429 1.40e-13 73.2
MS.gene69507.t1 AT3G51360 24.852 338 198 15 131 444 71 376 3.58e-12 68.6
MS.gene69507.t1 AT3G51360 24.852 338 198 15 131 444 81 386 4.05e-12 68.6
MS.gene69507.t1 AT3G12700 31.683 101 60 2 121 213 86 185 4.93e-12 66.6
MS.gene69507.t1 AT3G12700 33.696 92 52 2 130 213 95 185 4.95e-12 66.2
MS.gene69507.t1 AT5G24820 24.219 384 236 15 142 501 47 399 1.79e-11 66.6

Find 81 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCTGCACTTTCAATAAT+TGG 0.146871 3.1:+32457113 MS.gene69507:CDS
ACATTATAACATGGCTTTAA+AGG 0.166850 3.1:-32456951 None:intergenic
AAAGATGTGAAATTCAAATT+AGG 0.176046 3.1:-32456696 None:intergenic
GAAATGGAATTGCCTGAATT+TGG 0.207197 3.1:+32456987 MS.gene69507:CDS
CTCTCAAATTCTGAGAAATT+TGG 0.225386 3.1:+32455799 MS.gene69507:CDS
AGCATTCATGAAGAAAATTA+AGG 0.230380 3.1:+32456902 MS.gene69507:CDS
ATTATTGAAAGTGCAGATTT+AGG 0.236224 3.1:-32457110 None:intergenic
GTTAGTAGCAAATTGTTATT+TGG 0.249591 3.1:+32456648 MS.gene69507:CDS
TTAAAAGAAGTGGAATCTTT+AGG 0.256516 3.1:-32456261 None:intergenic
TTAACTGCAAAATCAGAAAA+TGG 0.275727 3.1:-32455892 None:intergenic
TAAACTTCATTGCACTTCAT+TGG 0.308323 3.1:-32457200 None:intergenic
ACACCGGTAGTGATCTTAAC+TGG 0.316215 3.1:+32456184 MS.gene69507:CDS
CTGCAATCAGCATTTAAGTT+TGG 0.319954 3.1:-32455775 None:intergenic
GAATTCAAACTCTGCATAGA+AGG 0.326275 3.1:+32455926 MS.gene69507:CDS
AAGATGTGAAATTCAAATTA+GGG 0.331531 3.1:-32456695 None:intergenic
GTACCCTTGAAATGGAGTTT+TGG 0.336827 3.1:-32455966 None:intergenic
CGCGGCGCTTCCGGTTTGTT+AGG 0.341387 3.1:+32456528 MS.gene69507:CDS
ATCTGATGATGTTGTTTGTT+TGG 0.342174 3.1:+32457073 MS.gene69507:CDS
TTGAACATGGATTTCCCATT+TGG 0.342241 3.1:-32456453 None:intergenic
AAACTATTCAACTCAGCTTA+TGG 0.349947 3.1:+32456068 MS.gene69507:CDS
TCACAACTTCAAAATCTCTA+TGG 0.352356 3.1:+32456579 MS.gene69507:CDS
TTGGAATCAGGAGTCAGTTT+TGG 0.353601 3.1:+32456096 MS.gene69507:CDS
AAGCTGAGTTGAATAGTTTC+CGG 0.354869 3.1:-32456064 None:intergenic
AGCAACAGCCGGCGACTTAT+AGG 0.359865 3.1:-32456016 None:intergenic
ATCGTCGAAAATGTTATGTT+TGG 0.362495 3.1:+32456477 MS.gene69507:CDS
GAAATAAAATCTATTATAGT+TGG 0.366850 3.1:+32456753 MS.gene69507:CDS
GAAAACAATATTCCAAATTC+AGG 0.374885 3.1:-32456999 None:intergenic
GGACTATTTCGCGGCGCTTC+CGG 0.376570 3.1:+32456519 MS.gene69507:CDS
TTATGTTTGGTTGTGGTCAT+TGG 0.389748 3.1:+32456490 MS.gene69507:CDS
CTTATGGCCACCTTGGAATC+AGG 0.409948 3.1:+32456084 MS.gene69507:CDS
TGAATAAAATAATTCTCAAC+AGG 0.421496 3.1:-32457044 None:intergenic
ATTTCTATCCACAAGACAAT+AGG 0.426902 3.1:-32456613 None:intergenic
GTGAATCTTACAAAACCAAA+TGG 0.427826 3.1:+32456438 MS.gene69507:CDS
AAATTAAATGAAGATGGTTC+TGG 0.430404 3.1:+32456810 MS.gene69507:CDS
GAATCTTTAGGATCATAATA+TGG 0.449031 3.1:-32456249 None:intergenic
TGATCCTCCTCAGCCTTGTA+AGG 0.451019 3.1:+32456329 MS.gene69507:CDS
TTACAAGGCTGAGGAGGATC+AGG 0.451589 3.1:-32456327 None:intergenic
GCTTCCGGTTTGTTAGGATT+AGG 0.454470 3.1:+32456534 MS.gene69507:CDS
GGTTGTGGTCATTGGAATAG+AGG 0.457397 3.1:+32456498 MS.gene69507:CDS
GGTGGTACAATAATCGATTC+CGG 0.462773 3.1:+32456831 MS.gene69507:CDS
GCATTCATGAAGAAAATTAA+GGG 0.463799 3.1:+32456903 MS.gene69507:CDS
ATTTATGTTCTTAAAAGAAG+TGG 0.466330 3.1:-32456271 None:intergenic
AACTCAGCTTATGGCCACCT+TGG 0.470142 3.1:+32456077 MS.gene69507:CDS
ACTTGGAAATTAAATGAAGA+TGG 0.473531 3.1:+32456804 MS.gene69507:CDS
TGATTCTCCTCAGCCTTACA+AGG 0.481580 3.1:-32456342 None:intergenic
AAATTATCATTATCATCATA+AGG 0.482819 3.1:-32455856 None:intergenic
TCTTGAATAATCTCATAAGC+TGG 0.486082 3.1:-32456879 None:intergenic
TCTTCCTAATCCTAACAAAC+CGG 0.491064 3.1:-32456538 None:intergenic
TGTGAGTGGAGTTGAAGAAA+TGG 0.493535 3.1:+32456971 MS.gene69507:CDS
AGAACCAAAACTCCATTTCA+AGG 0.498520 3.1:+32455962 MS.gene69507:CDS
GGTGATAGTTCTAACACAAC+TGG 0.500373 3.1:+32456390 MS.gene69507:CDS
TAGTTTCCGGTGAAATGCTC+CGG 0.514093 3.1:-32456051 None:intergenic
ATCAGCATTTAAGTTTGGAA+TGG 0.519040 3.1:-32455770 None:intergenic
AAAATAAGTTAAAGTTGTAC+CGG 0.519231 3.1:-32456850 None:intergenic
CAAATTTCTCAGAATTTGAG+AGG 0.521993 3.1:-32455798 None:intergenic
TGTTGCTCTCGCAGCACTAC+CGG 0.524129 3.1:+32456032 MS.gene69507:CDS
TTTAATTTCCAAGTTTCTTG+TGG 0.526744 3.1:-32456795 None:intergenic
GAAAATGTTATGTTTGGTTG+TGG 0.526829 3.1:+32456483 MS.gene69507:CDS
ATAAAATCTATTATAGTTGG+TGG 0.527088 3.1:+32456756 MS.gene69507:CDS
TGAATCTTACAAAACCAAAT+GGG 0.529298 3.1:+32456439 MS.gene69507:CDS
TATTTCATCGATGTGTTCAT+CGG 0.534233 3.1:+32456129 MS.gene69507:CDS
GCACTACCGGAGCATTTCAC+CGG 0.534805 3.1:+32456045 MS.gene69507:CDS
TCCTCAGCCTTGTAAGGCTG+AGG 0.538561 3.1:+32456335 MS.gene69507:CDS
TCCTCAGCCTTACAAGGCTG+AGG 0.548640 3.1:-32456336 None:intergenic
AAAACTGACTCCTGATTCCA+AGG 0.558968 3.1:-32456094 None:intergenic
AATTCAAACTCTGCATAGAA+GGG 0.576488 3.1:+32455927 MS.gene69507:CDS
AAGCCATGTTATAATGTGAG+TGG 0.580470 3.1:+32456957 MS.gene69507:CDS
TGGAATAGAGGACTATTTCG+CGG 0.580688 3.1:+32456510 MS.gene69507:CDS
AATCCAGTTAAGATCACTAC+CGG 0.581468 3.1:-32456187 None:intergenic
AGTTGTGAAGCAAATGATAA+CGG 0.585887 3.1:-32456564 None:intergenic
GGTTTGTTAGGATTAGGAAG+AGG 0.594942 3.1:+32456540 MS.gene69507:CDS
AGTGCTGCGAGAGCAACAGC+CGG 0.595452 3.1:-32456027 None:intergenic
TCAGCCTTACAAGGCTGAGG+AGG 0.598858 3.1:-32456333 None:intergenic
ACAGAAGTCCTATAAGTCGC+CGG 0.604036 3.1:+32456008 MS.gene69507:CDS
GAACCAAAACTCCATTTCAA+GGG 0.615830 3.1:+32455963 MS.gene69507:CDS
ACTCCACTCACATTATAACA+TGG 0.619319 3.1:-32456960 None:intergenic
TTAAATGAAGATGGTTCTGG+TGG 0.619428 3.1:+32456813 MS.gene69507:CDS
TGATCAAAACAAGCATAACA+AGG 0.620119 3.1:-32456219 None:intergenic
CACATCGATGAAATACTCAC+CGG 0.645373 3.1:-32456121 None:intergenic
GATATGAAGAAATCTAGACT+TGG 0.667212 3.1:+32457170 MS.gene69507:CDS
ACTGACTCCTGATTCCAAGG+TGG 0.742727 3.1:-32456091 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATCATTATCATCATA+AGG - chr3.1:32455859-32455878 None:intergenic 15.0%
!! GAAATAAAATCTATTATAGT+TGG + chr3.1:32456753-32456772 MS.gene69507:CDS 15.0%
!! AAAATAAGTTAAAGTTGTAC+CGG - chr3.1:32456853-32456872 None:intergenic 20.0%
!! AAAGATGTGAAATTCAAATT+AGG - chr3.1:32456699-32456718 None:intergenic 20.0%
!! AAGATGTGAAATTCAAATTA+GGG - chr3.1:32456698-32456717 None:intergenic 20.0%
!! ATAAAATCTATTATAGTTGG+TGG + chr3.1:32456756-32456775 MS.gene69507:CDS 20.0%
!! ATTTATGTTCTTAAAAGAAG+TGG - chr3.1:32456274-32456293 None:intergenic 20.0%
!! TGAATAAAATAATTCTCAAC+AGG - chr3.1:32457047-32457066 None:intergenic 20.0%
!!! AGTATCAAAGAAAAATGTTT+TGG - chr3.1:32456162-32456181 None:intergenic 20.0%
!!! ATTTGATTGTAACTTTTGAA+TGG - chr3.1:32455825-32455844 None:intergenic 20.0%
! AAATCTGCACTTTCAATAAT+TGG + chr3.1:32457113-32457132 MS.gene69507:CDS 25.0%
! AAATTAAATGAAGATGGTTC+TGG + chr3.1:32456810-32456829 MS.gene69507:CDS 25.0%
! ACTTGGAAATTAAATGAAGA+TGG + chr3.1:32456804-32456823 MS.gene69507:CDS 25.0%
! AGCATTCATGAAGAAAATTA+AGG + chr3.1:32456902-32456921 MS.gene69507:CDS 25.0%
! GAAAACAATATTCCAAATTC+AGG - chr3.1:32457002-32457021 None:intergenic 25.0%
! GCATTCATGAAGAAAATTAA+GGG + chr3.1:32456903-32456922 MS.gene69507:CDS 25.0%
! TAAAAATACCACAAGAAACT+TGG + chr3.1:32456787-32456806 MS.gene69507:CDS 25.0%
! TGAATCTTACAAAACCAAAT+GGG + chr3.1:32456439-32456458 MS.gene69507:CDS 25.0%
! TTAAAAGAAGTGGAATCTTT+AGG - chr3.1:32456264-32456283 None:intergenic 25.0%
! TTAACTGCAAAATCAGAAAA+TGG - chr3.1:32455895-32455914 None:intergenic 25.0%
!! ATTATTGAAAGTGCAGATTT+AGG - chr3.1:32457113-32457132 None:intergenic 25.0%
!! GTTAGTAGCAAATTGTTATT+TGG + chr3.1:32456648-32456667 MS.gene69507:CDS 25.0%
!! TTGAATTTCACATCTTTTGT+TGG + chr3.1:32456702-32456721 MS.gene69507:CDS 25.0%
!! TTTAATTTCCAAGTTTCTTG+TGG - chr3.1:32456798-32456817 None:intergenic 25.0%
!!! ACATTATAACATGGCTTTAA+AGG - chr3.1:32456954-32456973 None:intergenic 25.0%
!!! ATCAAAGAAAAATGTTTTGG+TGG - chr3.1:32456159-32456178 None:intergenic 25.0%
!!! GAATCTTTAGGATCATAATA+TGG - chr3.1:32456252-32456271 None:intergenic 25.0%
!!! TATGCTTGTTTTGATCAAAA+TGG + chr3.1:32456225-32456244 MS.gene69507:CDS 25.0%
!!! TTGTTTGTTTGGCTATTTTA+GGG + chr3.1:32457084-32457103 MS.gene69507:CDS 25.0%
!!! TTTGGAATATTGTTTTCAGA+TGG + chr3.1:32457005-32457024 MS.gene69507:CDS 25.0%
AAACTATTCAACTCAGCTTA+TGG + chr3.1:32456068-32456087 MS.gene69507:CDS 30.0%
AATTCAAACTCTGCATAGAA+GGG + chr3.1:32455927-32455946 MS.gene69507:CDS 30.0%
AGTTGTGAAGCAAATGATAA+CGG - chr3.1:32456567-32456586 None:intergenic 30.0%
ATCGTCGAAAATGTTATGTT+TGG + chr3.1:32456477-32456496 MS.gene69507:CDS 30.0%
ATTTCTATCCACAAGACAAT+AGG - chr3.1:32456616-32456635 None:intergenic 30.0%
CAAATTTCTCAGAATTTGAG+AGG - chr3.1:32455801-32455820 None:intergenic 30.0%
GAAAATGTTATGTTTGGTTG+TGG + chr3.1:32456483-32456502 MS.gene69507:CDS 30.0%
GATATGAAGAAATCTAGACT+TGG + chr3.1:32457170-32457189 MS.gene69507:CDS 30.0%
GTGAATCTTACAAAACCAAA+TGG + chr3.1:32456438-32456457 MS.gene69507:CDS 30.0%
TAAACTTCATTGCACTTCAT+TGG - chr3.1:32457203-32457222 None:intergenic 30.0%
TATTTCATCGATGTGTTCAT+CGG + chr3.1:32456129-32456148 MS.gene69507:CDS 30.0%
TCACAACTTCAAAATCTCTA+TGG + chr3.1:32456579-32456598 MS.gene69507:CDS 30.0%
TCACCGTACCAATAAAAATA+CGG - chr3.1:32456375-32456394 None:intergenic 30.0%
TCTTGAATAATCTCATAAGC+TGG - chr3.1:32456882-32456901 None:intergenic 30.0%
TGATCAAAACAAGCATAACA+AGG - chr3.1:32456222-32456241 None:intergenic 30.0%
! AATTTCACATCTTTTGTTGG+TGG + chr3.1:32456705-32456724 MS.gene69507:CDS 30.0%
! ATCAGCATTTAAGTTTGGAA+TGG - chr3.1:32455773-32455792 None:intergenic 30.0%
! ATCTGATGATGTTGTTTGTT+TGG + chr3.1:32457073-32457092 MS.gene69507:CDS 30.0%
! ATTTTCGACGATTTTGAACA+TGG - chr3.1:32456469-32456488 None:intergenic 30.0%
! CTCTCAAATTCTGAGAAATT+TGG + chr3.1:32455799-32455818 MS.gene69507:CDS 30.0%
! TTCAAGGGTACAAAAAACAA+AGG + chr3.1:32455978-32455997 MS.gene69507:CDS 30.0%
! TTCATTTTCCTATTGTCTTG+TGG + chr3.1:32456605-32456624 MS.gene69507:CDS 30.0%
! TTTTCTTTGATACTTGACAC+CGG + chr3.1:32456168-32456187 MS.gene69507:CDS 30.0%
!! AAACGTGTCCGTATTTTTAT+TGG + chr3.1:32456364-32456383 MS.gene69507:CDS 30.0%
!! GTTGTTTGTTTGGCTATTTT+AGG + chr3.1:32457083-32457102 MS.gene69507:CDS 30.0%
!! TGTCCGTATTTTTATTGGTA+CGG + chr3.1:32456369-32456388 MS.gene69507:CDS 30.0%
!!! TGTTTTTTGTACCCTTGAAA+TGG - chr3.1:32455977-32455996 None:intergenic 30.0%
AAGCCATGTTATAATGTGAG+TGG + chr3.1:32456957-32456976 MS.gene69507:CDS 35.0%
AATCCAGTTAAGATCACTAC+CGG - chr3.1:32456190-32456209 None:intergenic 35.0%
ACTCCACTCACATTATAACA+TGG - chr3.1:32456963-32456982 None:intergenic 35.0%
AGAACCAAAACTCCATTTCA+AGG + chr3.1:32455962-32455981 MS.gene69507:CDS 35.0%
AGGGTACAAAAAACAAAGGA+AGG + chr3.1:32455982-32456001 MS.gene69507:CDS 35.0%
GAAATGGAATTGCCTGAATT+TGG + chr3.1:32456987-32457006 MS.gene69507:CDS 35.0%
GAACCAAAACTCCATTTCAA+GGG + chr3.1:32455963-32455982 MS.gene69507:CDS 35.0%
GAATTCAAACTCTGCATAGA+AGG + chr3.1:32455926-32455945 MS.gene69507:CDS 35.0%
TCTTCCTAATCCTAACAAAC+CGG - chr3.1:32456541-32456560 None:intergenic 35.0%
TTATGTTTGGTTGTGGTCAT+TGG + chr3.1:32456490-32456509 MS.gene69507:CDS 35.0%
TTGAACATGGATTTCCCATT+TGG - chr3.1:32456456-32456475 None:intergenic 35.0%
! AAGCTGAGTTGAATAGTTTC+CGG - chr3.1:32456067-32456086 None:intergenic 35.0%
! CTGCAATCAGCATTTAAGTT+TGG - chr3.1:32455778-32455797 None:intergenic 35.0%
!! TTAAATGAAGATGGTTCTGG+TGG + chr3.1:32456813-32456832 MS.gene69507:CDS 35.0%
CACATCGATGAAATACTCAC+CGG - chr3.1:32456124-32456143 None:intergenic 40.0%
TGGAATAGAGGACTATTTCG+CGG + chr3.1:32456510-32456529 MS.gene69507:CDS 40.0%
TGTGAGTGGAGTTGAAGAAA+TGG + chr3.1:32456971-32456990 MS.gene69507:CDS 40.0%
! AAAACTGACTCCTGATTCCA+AGG - chr3.1:32456097-32456116 None:intergenic 40.0%
! GGTGGTACAATAATCGATTC+CGG + chr3.1:32456831-32456850 MS.gene69507:CDS 40.0%
! GTACCCTTGAAATGGAGTTT+TGG - chr3.1:32455969-32455988 None:intergenic 40.0%
! GTTTTCAGATGGAGCTGTTT+GGG + chr3.1:32457016-32457035 MS.gene69507:CDS 40.0%
! TGTTTTCAGATGGAGCTGTT+TGG + chr3.1:32457015-32457034 MS.gene69507:CDS 40.0%
! TTGGAATCAGGAGTCAGTTT+TGG + chr3.1:32456096-32456115 MS.gene69507:CDS 40.0%
!! GGTGATAGTTCTAACACAAC+TGG + chr3.1:32456390-32456409 MS.gene69507:CDS 40.0%
!! GGTTTGTTAGGATTAGGAAG+AGG + chr3.1:32456540-32456559 MS.gene69507:CDS 40.0%
ACACCGGTAGTGATCTTAAC+TGG + chr3.1:32456184-32456203 MS.gene69507:CDS 45.0%
ACAGAAGTCCTATAAGTCGC+CGG + chr3.1:32456008-32456027 MS.gene69507:CDS 45.0%
GGTTGTGGTCATTGGAATAG+AGG + chr3.1:32456498-32456517 MS.gene69507:CDS 45.0%
TAGTTTCCGGTGAAATGCTC+CGG - chr3.1:32456054-32456073 None:intergenic 45.0%
TGATTCTCCTCAGCCTTACA+AGG - chr3.1:32456345-32456364 None:intergenic 45.0%
!! GCTTCCGGTTTGTTAGGATT+AGG + chr3.1:32456534-32456553 MS.gene69507:CDS 45.0%
!!! TCAGGAGTCAGTTTTGGTTC+CGG + chr3.1:32456102-32456121 MS.gene69507:CDS 45.0%
AACTCAGCTTATGGCCACCT+TGG + chr3.1:32456077-32456096 MS.gene69507:CDS 50.0%
CTTATGGCCACCTTGGAATC+AGG + chr3.1:32456084-32456103 MS.gene69507:CDS 50.0%
TTACAAGGCTGAGGAGGATC+AGG - chr3.1:32456330-32456349 None:intergenic 50.0%
! ACTGACTCCTGATTCCAAGG+TGG - chr3.1:32456094-32456113 None:intergenic 50.0%
! TGATCCTCCTCAGCCTTGTA+AGG + chr3.1:32456329-32456348 MS.gene69507:CDS 50.0%
AGCAACAGCCGGCGACTTAT+AGG - chr3.1:32456019-32456038 None:intergenic 55.0%
TCAGCCTTACAAGGCTGAGG+AGG - chr3.1:32456336-32456355 None:intergenic 55.0%
TCCTCAGCCTTACAAGGCTG+AGG - chr3.1:32456339-32456358 None:intergenic 55.0%
TGTTGCTCTCGCAGCACTAC+CGG + chr3.1:32456032-32456051 MS.gene69507:CDS 55.0%
! GCACTACCGGAGCATTTCAC+CGG + chr3.1:32456045-32456064 MS.gene69507:CDS 55.0%
! TCCTCAGCCTTGTAAGGCTG+AGG + chr3.1:32456335-32456354 MS.gene69507:CDS 55.0%
AGTGCTGCGAGAGCAACAGC+CGG - chr3.1:32456030-32456049 None:intergenic 60.0%
GGACTATTTCGCGGCGCTTC+CGG + chr3.1:32456519-32456538 MS.gene69507:CDS 60.0%
! CGCGGCGCTTCCGGTTTGTT+AGG + chr3.1:32456528-32456547 MS.gene69507:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 32455709 32457223 32455709 ID=MS.gene69507
chr3.1 mRNA 32455709 32457223 32455709 ID=MS.gene69507.t1;Parent=MS.gene69507
chr3.1 exon 32455709 32457223 32455709 ID=MS.gene69507.t1.exon1;Parent=MS.gene69507.t1
chr3.1 CDS 32455709 32457223 32455709 ID=cds.MS.gene69507.t1;Parent=MS.gene69507.t1
Gene Sequence

>MS.gene69507

ATGGTTTTGAAACTTTCTCTCATTCTTATTTTTCTTGTGTTACATTTCTTCACTTTAGAAGCCATTCCAAACTTAAATGCTGATTGCAGCCTCTCAAATTCTGAGAAATTTGGCCATTCAAAAGTTACAATCAAATCAAGTGAAGAGCCTTATGATGATAATGATAATTTTGAGTCTAAAAAACCATTTTCTGATTTTGCAGTTAAAGACTTGACAAGAATTCAAACTCTGCATAGAAGGGTCATTGAAAAGAAGAACCAAAACTCCATTTCAAGGGTACAAAAAACAAAGGAAGGATCACAGAAGTCCTATAAGTCGCCGGCTGTTGCTCTCGCAGCACTACCGGAGCATTTCACCGGAAACTATTCAACTCAGCTTATGGCCACCTTGGAATCAGGAGTCAGTTTTGGTTCCGGTGAGTATTTCATCGATGTGTTCATCGGTACGCCACCAAAACATTTTTCTTTGATACTTGACACCGGTAGTGATCTTAACTGGATTCAATGTGTTCCTTGTTATGCTTGTTTTGATCAAAATGGACCATATTATGATCCTAAAGATTCCACTTCTTTTAAGAACATAAATTGTCACGATTCGCGATGTCAACTCGTGTCATCGCCTGATCCTCCTCAGCCTTGTAAGGCTGAGGAGAATCAAACGTGTCCGTATTTTTATTGGTACGGTGATAGTTCTAACACAACTGGCGATTTCGCACTTGAAACATTTCGAGTGAATCTTACAAAACCAAATGGGAAATCCATGTTCAAAATCGTCGAAAATGTTATGTTTGGTTGTGGTCATTGGAATAGAGGACTATTTCGCGGCGCTTCCGGTTTGTTAGGATTAGGAAGAGGACCGTTATCATTTGCTTCACAACTTCAAAATCTCTATGGTCATTCATTTTCCTATTGTCTTGTGGATAGAAATAGCAATTCAAGTGTTAGTAGCAAATTGTTATTTGGCGAAAACAAAAAGCTTCTTAGTAACCCTAATTTGAATTTCACATCTTTTGTTGGTGGAAAAGAGAACACATTTTACTATGTTGAAATAAAATCTATTATAGTTGGTGGTGAAGTGCTAAAAATACCACAAGAAACTTGGAAATTAAATGAAGATGGTTCTGGTGGTACAATAATCGATTCCGGTACAACTTTAACTTATTTTGCTAAACCAGCTTATGAGATTATTCAAGAAGCATTCATGAAGAAAATTAAGGGTTATGCAATTGTTGAAAGTTTTGCACCTTTAAAGCCATGTTATAATGTGAGTGGAGTTGAAGAAATGGAATTGCCTGAATTTGGAATATTGTTTTCAGATGGAGCTGTTTGGGATTTTCCTGTTGAGAATTATTTTATTCAGATTGAATCTGATGATGTTGTTTGTTTGGCTATTTTAGGGACTCCTAAATCTGCACTTTCAATAATTGGAAATTATCAGCAACAGAATTTTCAGATTTTGTATGATATGAAGAAATCTAGACTTGGTTATGCTCCAATGAAGTGCAATGAAGTTTAA
Protein sequence

>MS.gene69507.t1

MVLKLSLILIFLVLHFFTLEAIPNLNADCSLSNSEKFGHSKVTIKSSEEPYDDNDNFESKKPFSDFAVKDLTRIQTLHRRVIEKKNQNSISRVQKTKEGSQKSYKSPAVALAALPEHFTGNYSTQLMATLESGVSFGSGEYFIDVFIGTPPKHFSLILDTGSDLNWIQCVPCYACFDQNGPYYDPKDSTSFKNINCHDSRCQLVSSPDPPQPCKAEENQTCPYFYWYGDSSNTTGDFALETFRVNLTKPNGKSMFKIVENVMFGCGHWNRGLFRGASGLLGLGRGPLSFASQLQNLYGHSFSYCLVDRNSNSSVSSKLLFGENKKLLSNPNLNFTSFVGGKENTFYYVEIKSIIVGGEVLKIPQETWKLNEDGSGGTIIDSGTTLTYFAKPAYEIIQEAFMKKIKGYAIVESFAPLKPCYNVSGVEEMELPEFGILFSDGAVWDFPVENYFIQIESDDVVCLAILGTPKSALSIIGNYQQQNFQILYDMKKSRLGYAPMKCNEV