Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69674.t1 | XP_013445996.1 | 80 | 85 | 11 | 2 | 50 | 129 | 79 | 162 | 1.60E-26 | 129 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69674.t1 | A0A072TT50 | 80.0 | 85 | 11 | 2 | 50 | 129 | 79 | 162 | 1.1e-26 | 129.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049239 | MS.gene69674 | 0.846042 | 2.78E-59 | -1.69E-46 |
| MS.gene051539 | MS.gene69674 | 0.819466 | 1.14E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69674.t1 | MTR_8g064760 | 73.288 | 146 | 4 | 3 | 18 | 129 | 18 | 162 | 8.52e-66 | 197 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCATTCAAATGGATCTTT+TGG | 0.191350 | 8.3:+37232748 | MS.gene69674:CDS |
| CCTCTAGGCCTAGTAATTAT+TGG | 0.294250 | 8.3:-37233003 | None:intergenic |
| CTGCCTACTCGACGGCTTAA+CGG | 0.302986 | 8.3:-37232911 | None:intergenic |
| TGCTATAATACTAGTGATTT+CGG | 0.341197 | 8.3:+37232789 | MS.gene69674:CDS |
| TGCCTACTCGACGGCTTAAC+GGG | 0.376180 | 8.3:-37232910 | None:intergenic |
| GTTGATTTGTGTTAGTTGTT+GGG | 0.398380 | 8.3:-37232717 | None:intergenic |
| CAAAAGATCCATTTGAATGA+TGG | 0.405902 | 8.3:-37232747 | None:intergenic |
| GGTTGATTTGTGTTAGTTGT+TGG | 0.421845 | 8.3:-37232718 | None:intergenic |
| GTTGTTGGGTAAACTTGAAC+AGG | 0.430980 | 8.3:-37232703 | None:intergenic |
| CCATCTTCCCATCATTCAAA+TGG | 0.441379 | 8.3:+37232739 | MS.gene69674:CDS |
| AATCCAAATATGTCTATTCA+AGG | 0.460611 | 8.3:+37232655 | None:intergenic |
| AAAAGATCCATTTGAATGAT+GGG | 0.472353 | 8.3:-37232746 | None:intergenic |
| CAACCACATGGTCATGGTAA+TGG | 0.479592 | 8.3:+37233060 | MS.gene69674:CDS |
| TTTGAACCACCACGCACTGC+AGG | 0.513780 | 8.3:+37233033 | MS.gene69674:CDS |
| CCATTTGAATGATGGGAAGA+TGG | 0.517579 | 8.3:-37232739 | None:intergenic |
| GGTTCAAAAGCAAGTCCTCT+AGG | 0.529478 | 8.3:-37233018 | None:intergenic |
| TGCCTCCACCAATAATTACT+AGG | 0.540441 | 8.3:+37232995 | MS.gene69674:CDS |
| CTAGGCCTAGTAATTATTGG+TGG | 0.551916 | 8.3:-37233000 | None:intergenic |
| AGAGAAGATGACATTGAACT+TGG | 0.557715 | 8.3:+37232958 | MS.gene69674:CDS |
| GAAAGTGATCAGCATAGAGC+AGG | 0.557844 | 8.3:+37233117 | MS.gene69674:CDS |
| ATCTGTTGCTGCCTACTCGA+CGG | 0.571035 | 8.3:-37232919 | None:intergenic |
| GGCCTAGTAATTATTGGTGG+AGG | 0.576499 | 8.3:-37232997 | None:intergenic |
| GGATCTCAACCACATGGTCA+TGG | 0.591514 | 8.3:+37233054 | MS.gene69674:CDS |
| TAAACTTGAACAGGTTGTTG+TGG | 0.654880 | 8.3:-37232694 | None:intergenic |
| CCAATAATTACTAGGCCTAG+AGG | 0.665419 | 8.3:+37233003 | MS.gene69674:CDS |
| GGCCCGTTAAGCCGTCGAGT+AGG | 0.668761 | 8.3:+37232908 | MS.gene69674:CDS |
| AAATCACTAGTATTATAGCA+AGG | 0.678683 | 8.3:-37232786 | None:intergenic |
| TGAGATCCTGCAGTGCGTGG+TGG | 0.678937 | 8.3:-37233039 | None:intergenic |
| GGTTGAGATCCTGCAGTGCG+TGG | 0.691635 | 8.3:-37233042 | None:intergenic |
| ACTGCAGGATCTCAACCACA+TGG | 0.729651 | 8.3:+37233048 | MS.gene69674:CDS |
| TTACCATTACCATGACCATG+TGG | 0.751168 | 8.3:-37233063 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAGATCCATTTGAATGAT+GGG | - | chr8.3:37232749-37232768 | None:intergenic | 25.0% |
| ! | AAATCACTAGTATTATAGCA+AGG | - | chr8.3:37232789-37232808 | None:intergenic | 25.0% |
| ! | TGCTATAATACTAGTGATTT+CGG | + | chr8.3:37232789-37232808 | MS.gene69674:CDS | 25.0% |
| !! | ATCATTCAAATGGATCTTTT+GGG | + | chr8.3:37232749-37232768 | MS.gene69674:CDS | 25.0% |
| CAAAAGATCCATTTGAATGA+TGG | - | chr8.3:37232750-37232769 | None:intergenic | 30.0% | |
| ! | CATCATTCAAATGGATCTTT+TGG | + | chr8.3:37232748-37232767 | MS.gene69674:CDS | 30.0% |
| !! | GTTGATTTGTGTTAGTTGTT+GGG | - | chr8.3:37232720-37232739 | None:intergenic | 30.0% |
| !!! | TGTCGTTGTTGTTGTTTTTA+TGG | - | chr8.3:37232855-37232874 | None:intergenic | 30.0% |
| AGAGAAGATGACATTGAACT+TGG | + | chr8.3:37232958-37232977 | MS.gene69674:CDS | 35.0% | |
| TAAACTTGAACAGGTTGTTG+TGG | - | chr8.3:37232697-37232716 | None:intergenic | 35.0% | |
| !! | GGTTGATTTGTGTTAGTTGT+TGG | - | chr8.3:37232721-37232740 | None:intergenic | 35.0% |
| !!! | TGTTGTTTTTATGGTAGCGT+CGG | - | chr8.3:37232846-37232865 | None:intergenic | 35.0% |
| CCAATAATTACTAGGCCTAG+AGG | + | chr8.3:37233003-37233022 | MS.gene69674:CDS | 40.0% | |
| CCATCTTCCCATCATTCAAA+TGG | + | chr8.3:37232739-37232758 | MS.gene69674:CDS | 40.0% | |
| CCTCTAGGCCTAGTAATTAT+TGG | - | chr8.3:37233006-37233025 | None:intergenic | 40.0% | |
| CTAGGCCTAGTAATTATTGG+TGG | - | chr8.3:37233003-37233022 | None:intergenic | 40.0% | |
| GTTGTTGGGTAAACTTGAAC+AGG | - | chr8.3:37232706-37232725 | None:intergenic | 40.0% | |
| TGCCTCCACCAATAATTACT+AGG | + | chr8.3:37232995-37233014 | MS.gene69674:CDS | 40.0% | |
| TTACCATTACCATGACCATG+TGG | - | chr8.3:37233066-37233085 | None:intergenic | 40.0% | |
| ! | CCATTTGAATGATGGGAAGA+TGG | - | chr8.3:37232742-37232761 | None:intergenic | 40.0% |
| !! | TCGGTAGTTGCTTGTTTTCT+CGG | + | chr8.3:37232808-37232827 | MS.gene69674:intron | 40.0% |
| !!! | GGCTGTTGTGTTGATGATTT+TGG | - | chr8.3:37232879-37232898 | None:intergenic | 40.0% |
| CAACCACATGGTCATGGTAA+TGG | + | chr8.3:37233060-37233079 | MS.gene69674:CDS | 45.0% | |
| GAAAGTGATCAGCATAGAGC+AGG | + | chr8.3:37233117-37233136 | MS.gene69674:CDS | 45.0% | |
| GGCCTAGTAATTATTGGTGG+AGG | - | chr8.3:37233000-37233019 | None:intergenic | 45.0% | |
| GGTTCAAAAGCAAGTCCTCT+AGG | - | chr8.3:37233021-37233040 | None:intergenic | 45.0% | |
| AACACAACAGCCTGCAGAGA+CGG | + | chr8.3:37232887-37232906 | MS.gene69674:intron | 50.0% | |
| ACTGCAGGATCTCAACCACA+TGG | + | chr8.3:37233048-37233067 | MS.gene69674:CDS | 50.0% | |
| ATCTGTTGCTGCCTACTCGA+CGG | - | chr8.3:37232922-37232941 | None:intergenic | 50.0% | |
| GGATCTCAACCACATGGTCA+TGG | + | chr8.3:37233054-37233073 | MS.gene69674:CDS | 50.0% | |
| !! | TATGGTAGCGTCGGTTGCAA+AGG | - | chr8.3:37232837-37232856 | None:intergenic | 50.0% |
| CTGCCTACTCGACGGCTTAA+CGG | - | chr8.3:37232914-37232933 | None:intergenic | 55.0% | |
| TGCCTACTCGACGGCTTAAC+GGG | - | chr8.3:37232913-37232932 | None:intergenic | 55.0% | |
| TTTGAACCACCACGCACTGC+AGG | + | chr8.3:37233033-37233052 | MS.gene69674:CDS | 55.0% | |
| GGTTGAGATCCTGCAGTGCG+TGG | - | chr8.3:37233045-37233064 | None:intergenic | 60.0% | |
| TGAGATCCTGCAGTGCGTGG+TGG | - | chr8.3:37233042-37233061 | None:intergenic | 60.0% | |
| GGCCCGTTAAGCCGTCGAGT+AGG | + | chr8.3:37232908-37232927 | MS.gene69674:CDS | 65.0% | |
| ! | GCTTAACGGGCCGTCTCTGC+AGG | - | chr8.3:37232900-37232919 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 37232664 | 37233146 | 37232664 | ID=MS.gene69674 |
| chr8.3 | mRNA | 37232664 | 37233146 | 37232664 | ID=MS.gene69674.t1;Parent=MS.gene69674 |
| chr8.3 | exon | 37232664 | 37232810 | 37232664 | ID=MS.gene69674.t1.exon1;Parent=MS.gene69674.t1 |
| chr8.3 | CDS | 37232664 | 37232810 | 37232664 | ID=cds.MS.gene69674.t1;Parent=MS.gene69674.t1 |
| chr8.3 | exon | 37232904 | 37233146 | 37232904 | ID=MS.gene69674.t1.exon2;Parent=MS.gene69674.t1 |
| chr8.3 | CDS | 37232904 | 37233146 | 37232904 | ID=cds.MS.gene69674.t1;Parent=MS.gene69674.t1 |
| Gene Sequence |
| Protein sequence |
