Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69676.t1 | AFK35673.1 | 81.2 | 80 | 3 | 1 | 1 | 68 | 13 | 92 | 1.30E-25 | 125.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69676.t1 | Q43207 | 66.7 | 75 | 12 | 2 | 6 | 68 | 21 | 94 | 2.4e-17 | 89.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69676.t1 | G7LF61 | 81.2 | 80 | 3 | 1 | 1 | 68 | 13 | 92 | 9.1e-26 | 125.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058515 | MS.gene69676 | 0.806369 | 8.50E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69676.t1 | MTR_8g012340 | 78.750 | 80 | 5 | 1 | 1 | 68 | 13 | 92 | 9.25e-25 | 98.2 |
| MS.gene69676.t1 | MTR_8g080980 | 57.778 | 90 | 16 | 2 | 1 | 68 | 1 | 90 | 4.71e-23 | 93.2 |
| MS.gene69676.t1 | MTR_7g053470 | 54.651 | 86 | 21 | 2 | 1 | 68 | 1 | 86 | 1.82e-14 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69676.t1 | AT5G48570 | 63.889 | 72 | 14 | 1 | 10 | 69 | 29 | 100 | 1.15e-20 | 86.3 |
| MS.gene69676.t1 | AT3G25230 | 70.769 | 65 | 6 | 2 | 16 | 68 | 28 | 91 | 6.50e-19 | 81.3 |
| MS.gene69676.t1 | AT3G25230 | 70.769 | 65 | 6 | 2 | 16 | 68 | 28 | 91 | 7.56e-19 | 81.3 |
Find 30 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTGTGTAGTTCATTATAC+TGG | 0.254741 | 8.3:+37197648 | MS.gene69676:intron |
| ACTACTTTCAATTTCACTCT+TGG | 0.361943 | 8.3:+37197717 | MS.gene69676:CDS |
| AGTTTGATTCTAGCAGAGAT+AGG | 0.388381 | 8.3:+37197691 | MS.gene69676:CDS |
| TATACTGGAACGTTGCTTGA+TGG | 0.393092 | 8.3:+37197663 | MS.gene69676:CDS |
| TCCCACATCGTACTATTATC+AGG | 0.413728 | 8.3:+37198291 | MS.gene69676:CDS |
| CTTCAAGAATAATAATAGAT+AGG | 0.425201 | 8.3:-37197915 | None:intergenic |
| GAAGTCGAAGTCGTCTTTCA+TGG | 0.449885 | 8.3:-37197441 | None:intergenic |
| AAGAAGAAGCTTCTCAAAGA+AGG | 0.467998 | 8.3:+37197530 | MS.gene69676:CDS |
| GTTTGATTCTAGCAGAGATA+GGG | 0.475863 | 8.3:+37197692 | MS.gene69676:CDS |
| TAGGCAGATGGAAGGTGATA+AGG | 0.476180 | 8.3:-37197896 | None:intergenic |
| ATCTATTATTATTCTTGAAG+AGG | 0.490271 | 8.3:+37197918 | MS.gene69676:CDS |
| GACTTCGACTTCGCCGGTGC+CGG | 0.516399 | 8.3:+37197452 | MS.gene69676:CDS |
| AATGAAATTAAAAGCATGGC+AGG | 0.519602 | 8.3:+37198259 | MS.gene69676:CDS |
| AATTGGATTATCGCCGGCAC+CGG | 0.525961 | 8.3:-37197465 | None:intergenic |
| GTGGGTACTAGAGAATTCAT+AGG | 0.534544 | 8.3:+37198317 | MS.gene69676:CDS |
| TTCAATTTCACTCTTGGACA+AGG | 0.538952 | 8.3:+37197723 | MS.gene69676:CDS |
| GTCAAATGAAATTAAAAGCA+TGG | 0.545516 | 8.3:+37198255 | MS.gene69676:CDS |
| AATAATAATAGATAGGCAGA+TGG | 0.568838 | 8.3:-37197908 | None:intergenic |
| CTTGAGAATTGGATTATCGC+CGG | 0.576084 | 8.3:-37197471 | None:intergenic |
| CATATGTAGACACTGTCATA+GGG | 0.583382 | 8.3:-37198348 | None:intergenic |
| TTTGATTCTAGCAGAGATAG+GGG | 0.593989 | 8.3:+37197693 | MS.gene69676:CDS |
| TCGTACTATTATCAGGCAAG+TGG | 0.597519 | 8.3:+37198298 | MS.gene69676:CDS |
| AAAGACGACTTCGACTTCGC+CGG | 0.605504 | 8.3:+37197446 | MS.gene69676:CDS |
| AAGCTTCTCAAAGAAGGTGA+AGG | 0.613170 | 8.3:+37197536 | MS.gene69676:CDS |
| CGGCGATAATCCAATTCTCA+AGG | 0.616014 | 8.3:+37197472 | MS.gene69676:CDS |
| TGCCTGATAATAGTACGATG+TGG | 0.623695 | 8.3:-37198293 | None:intergenic |
| ATAATAGATAGGCAGATGGA+AGG | 0.627317 | 8.3:-37197904 | None:intergenic |
| CGTACTATTATCAGGCAAGT+GGG | 0.636740 | 8.3:+37198299 | MS.gene69676:CDS |
| GCCTGATAATAGTACGATGT+GGG | 0.647015 | 8.3:-37198292 | None:intergenic |
| GATAATCCAATTCTCAAGGT+CGG | 0.697498 | 8.3:+37197476 | MS.gene69676:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGATTTTTGTTATTTTAAT+TGG | + | chr8.3:37197770-37197789 | MS.gene69676:intron | 10.0% |
| !! | AAATCAAACATAGAAATTAA+CGG | - | chr8.3:37197798-37197817 | None:intergenic | 15.0% |
| !!! | TTAATTTCTATGTTTGATTT+AGG | + | chr8.3:37197798-37197817 | MS.gene69676:intron | 15.0% |
| !! | ATCTATTATTATTCTTGAAG+AGG | + | chr8.3:37197918-37197937 | MS.gene69676:CDS | 20.0% |
| !! | CTTCAAGAATAATAATAGAT+AGG | - | chr8.3:37197918-37197937 | None:intergenic | 20.0% |
| !!! | TCTTTTATTTTCTTCACAAA+TGG | - | chr8.3:37198159-37198178 | None:intergenic | 20.0% |
| ! | AATAATAATAGATAGGCAGA+TGG | - | chr8.3:37197911-37197930 | None:intergenic | 25.0% |
| ! | GATTTCAAACAATAAAAAGC+AGG | + | chr8.3:37198007-37198026 | MS.gene69676:intron | 25.0% |
| ! | GTCAAATGAAATTAAAAGCA+TGG | + | chr8.3:37198255-37198274 | MS.gene69676:CDS | 25.0% |
| ! | TGTCATCAATAACATATTGT+TGG | + | chr8.3:37197970-37197989 | MS.gene69676:intron | 25.0% |
| !! | ACCTTTAATTTCCTCATAAT+AGG | - | chr8.3:37198100-37198119 | None:intergenic | 25.0% |
| !! | CCTTTAATTTCCTCATAATA+GGG | - | chr8.3:37198099-37198118 | None:intergenic | 25.0% |
| !! | TGTTTGATTTAGGTTATGTT+TGG | + | chr8.3:37197808-37197827 | MS.gene69676:intron | 25.0% |
| !!! | AGAGGTAAATTTTAAAAGTC+AGG | + | chr8.3:37197936-37197955 | MS.gene69676:intron | 25.0% |
| AATCAACGAAGAAAGAACAT+AGG | - | chr8.3:37197588-37197607 | None:intergenic | 30.0% | |
| ACTACTTTCAATTTCACTCT+TGG | + | chr8.3:37197717-37197736 | MS.gene69676:CDS | 30.0% | |
| CCCTATTATGAGGAAATTAA+AGG | + | chr8.3:37198096-37198115 | MS.gene69676:intron | 30.0% | |
| TTCTGTGTAGTTCATTATAC+TGG | + | chr8.3:37197648-37197667 | MS.gene69676:intron | 30.0% | |
| ! | AATGAAATTAAAAGCATGGC+AGG | + | chr8.3:37198259-37198278 | MS.gene69676:CDS | 30.0% |
| ! | CATTTGCTGATAACTACAAA+AGG | - | chr8.3:37198067-37198086 | None:intergenic | 30.0% |
| !! | TGAAAAGTGCTTATCATATC+AGG | + | chr8.3:37197862-37197881 | MS.gene69676:intron | 30.0% |
| AAAAAGGAAATCGGAAAGCA+AGG | + | chr8.3:37197503-37197522 | MS.gene69676:CDS | 35.0% | |
| AAGAAGAAGCTTCTCAAAGA+AGG | + | chr8.3:37197530-37197549 | MS.gene69676:CDS | 35.0% | |
| AGTTTGATTCTAGCAGAGAT+AGG | + | chr8.3:37197691-37197710 | MS.gene69676:CDS | 35.0% | |
| ATAATAGATAGGCAGATGGA+AGG | - | chr8.3:37197907-37197926 | None:intergenic | 35.0% | |
| GAGATATGATCCCTATTATG+AGG | + | chr8.3:37198086-37198105 | MS.gene69676:intron | 35.0% | |
| GATAATCCAATTCTCAAGGT+CGG | + | chr8.3:37197476-37197495 | MS.gene69676:CDS | 35.0% | |
| GTCGGTGAAAAAAAGGAAAT+CGG | + | chr8.3:37197494-37197513 | MS.gene69676:CDS | 35.0% | |
| GTTTGATTCTAGCAGAGATA+GGG | + | chr8.3:37197692-37197711 | MS.gene69676:CDS | 35.0% | |
| TTCAATTTCACTCTTGGACA+AGG | + | chr8.3:37197723-37197742 | MS.gene69676:CDS | 35.0% | |
| TTTGATTCTAGCAGAGATAG+GGG | + | chr8.3:37197693-37197712 | MS.gene69676:CDS | 35.0% | |
| !! | GCTGCTTACTTTTGATATTG+TGG | + | chr8.3:37198185-37198204 | MS.gene69676:intron | 35.0% |
| AAGCTTCTCAAAGAAGGTGA+AGG | + | chr8.3:37197536-37197555 | MS.gene69676:CDS | 40.0% | |
| CGTACTATTATCAGGCAAGT+GGG | + | chr8.3:37198299-37198318 | MS.gene69676:CDS | 40.0% | |
| CTTGAGAATTGGATTATCGC+CGG | - | chr8.3:37197474-37197493 | None:intergenic | 40.0% | |
| GCCTGATAATAGTACGATGT+GGG | - | chr8.3:37198295-37198314 | None:intergenic | 40.0% | |
| GTGGGTACTAGAGAATTCAT+AGG | + | chr8.3:37198317-37198336 | MS.gene69676:CDS | 40.0% | |
| TATACTGGAACGTTGCTTGA+TGG | + | chr8.3:37197663-37197682 | MS.gene69676:CDS | 40.0% | |
| TCCCACATCGTACTATTATC+AGG | + | chr8.3:37198291-37198310 | MS.gene69676:CDS | 40.0% | |
| TCGTACTATTATCAGGCAAG+TGG | + | chr8.3:37198298-37198317 | MS.gene69676:CDS | 40.0% | |
| TCTCAAAGAAGGTGAAGGTT+GGG | + | chr8.3:37197541-37197560 | MS.gene69676:intron | 40.0% | |
| TCTCAAGGTCGGTGAAAAAA+AGG | + | chr8.3:37197487-37197506 | MS.gene69676:CDS | 40.0% | |
| TGCCTGATAATAGTACGATG+TGG | - | chr8.3:37198296-37198315 | None:intergenic | 40.0% | |
| TTCTCAAAGAAGGTGAAGGT+TGG | + | chr8.3:37197540-37197559 | MS.gene69676:intron | 40.0% | |
| ! | TTTTCACCGACCTTGAGAAT+TGG | - | chr8.3:37197485-37197504 | None:intergenic | 40.0% |
| AGGTTAGTGCATTGGAACGA+CGG | + | chr8.3:37197743-37197762 | MS.gene69676:intron | 45.0% | |
| CGGCGATAATCCAATTCTCA+AGG | + | chr8.3:37197472-37197491 | MS.gene69676:CDS | 45.0% | |
| CTTGGACAAGGTTAGTGCAT+TGG | + | chr8.3:37197735-37197754 | MS.gene69676:intron | 45.0% | |
| TAGGCAGATGGAAGGTGATA+AGG | - | chr8.3:37197899-37197918 | None:intergenic | 45.0% | |
| AAAGACGACTTCGACTTCGC+CGG | + | chr8.3:37197446-37197465 | MS.gene69676:CDS | 50.0% | |
| AATTGGATTATCGCCGGCAC+CGG | - | chr8.3:37197468-37197487 | None:intergenic | 50.0% | |
| GGTTGGGATGCTCATGATGT+TGG | + | chr8.3:37197557-37197576 | MS.gene69676:intron | 50.0% | |
| GACTTCGACTTCGCCGGTGC+CGG | + | chr8.3:37197452-37197471 | MS.gene69676:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 37197443 | 37198355 | 37197443 | ID=MS.gene69676 |
| chr8.3 | mRNA | 37197443 | 37198355 | 37197443 | ID=MS.gene69676.t1;Parent=MS.gene69676 |
| chr8.3 | exon | 37197443 | 37197557 | 37197443 | ID=MS.gene69676.t1.exon1;Parent=MS.gene69676.t1 |
| chr8.3 | CDS | 37197443 | 37197557 | 37197443 | ID=cds.MS.gene69676.t1;Parent=MS.gene69676.t1 |
| chr8.3 | exon | 37197658 | 37197744 | 37197658 | ID=MS.gene69676.t1.exon2;Parent=MS.gene69676.t1 |
| chr8.3 | CDS | 37197658 | 37197744 | 37197658 | ID=cds.MS.gene69676.t1;Parent=MS.gene69676.t1 |
| chr8.3 | exon | 37197884 | 37197939 | 37197884 | ID=MS.gene69676.t1.exon3;Parent=MS.gene69676.t1 |
| chr8.3 | CDS | 37197884 | 37197939 | 37197884 | ID=cds.MS.gene69676.t1;Parent=MS.gene69676.t1 |
| chr8.3 | exon | 37198242 | 37198355 | 37198242 | ID=MS.gene69676.t1.exon4;Parent=MS.gene69676.t1 |
| chr8.3 | CDS | 37198242 | 37198355 | 37198242 | ID=cds.MS.gene69676.t1;Parent=MS.gene69676.t1 |
| Gene Sequence |
| Protein sequence |
