AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70228

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70228.t1 MTR_8g093530 98.394 436 5 1 1 436 1 434 0.0 885
MS.gene70228.t1 MTR_3g090120 53.545 409 168 3 34 423 59 464 8.45e-157 452
MS.gene70228.t1 MTR_7g094750 44.847 359 179 7 92 434 91 446 7.22e-104 316
MS.gene70228.t1 MTR_2g022560 42.655 354 184 6 92 429 97 447 1.84e-100 307
MS.gene70228.t1 MTR_7g109550 40.841 333 189 3 92 418 137 467 1.45e-87 275
MS.gene70228.t1 MTR_7g094750 51.899 237 112 2 92 327 91 326 7.03e-87 268
MS.gene70228.t1 MTR_1g058380 35.539 408 239 10 44 430 5 409 4.59e-83 261
MS.gene70228.t1 MTR_4g121560 37.709 358 206 5 80 422 102 457 5.48e-82 260
MS.gene70228.t1 MTR_0136s0010 38.920 352 190 6 89 421 167 512 7.85e-81 259
MS.gene70228.t1 MTR_4g126950 35.735 347 206 5 89 421 106 449 1.76e-80 256
MS.gene70228.t1 MTR_5g059400 36.103 349 203 4 88 419 171 516 4.67e-79 254
MS.gene70228.t1 MTR_1g116130 36.103 349 203 4 88 419 171 516 4.67e-79 254
MS.gene70228.t1 MTR_7g093450 38.000 350 192 6 89 419 245 588 5.74e-79 256
MS.gene70228.t1 MTR_5g033800 36.337 344 203 4 90 419 143 484 1.67e-78 252
MS.gene70228.t1 MTR_8g069790 35.429 350 210 5 84 419 170 517 1.48e-75 245
MS.gene70228.t1 MTR_3g464570 34.483 348 195 6 92 419 43 377 2.78e-70 227
MS.gene70228.t1 MTR_2g073500 35.756 344 193 9 87 421 24 348 7.38e-66 214
MS.gene70228.t1 MTR_5g033800 41.004 239 139 1 90 328 143 379 1.51e-64 212
MS.gene70228.t1 MTR_4g016940 32.102 352 208 7 90 418 84 427 8.68e-64 211
MS.gene70228.t1 MTR_1g050448 31.633 392 230 10 44 418 5 375 3.90e-63 208
MS.gene70228.t1 MTR_5g034810 33.894 357 209 8 69 418 16 352 1.24e-62 206
MS.gene70228.t1 MTR_4g088030 34.030 335 198 7 92 419 33 351 2.01e-62 206
MS.gene70228.t1 MTR_4g087730 33.828 337 202 6 89 419 42 363 8.71e-62 204
MS.gene70228.t1 MTR_3g021060 31.642 335 208 7 90 418 44 363 9.43e-62 204
MS.gene70228.t1 MTR_2g015720 31.148 366 231 7 59 418 8 358 3.42e-61 203
MS.gene70228.t1 MTR_1g112210 32.845 341 202 6 90 418 57 382 4.22e-60 201
MS.gene70228.t1 MTR_5g071350 31.522 368 202 7 83 419 52 400 1.19e-59 200
MS.gene70228.t1 MTR_2g073510 34.821 336 191 10 90 414 31 349 1.04e-58 196
MS.gene70228.t1 MTR_8g463790 32.493 357 203 10 92 423 71 414 1.61e-58 197
MS.gene70228.t1 MTR_1g112210 33.724 341 186 7 90 418 57 369 1.62e-58 196
MS.gene70228.t1 MTR_3g049330 33.134 335 201 7 92 418 31 350 1.62e-58 196
MS.gene70228.t1 MTR_1g111010 35.799 338 184 13 90 419 30 342 2.91e-58 195
MS.gene70228.t1 MTR_4g014440 30.168 358 215 8 89 419 59 408 3.81e-58 196
MS.gene70228.t1 MTR_3g070900 31.161 353 212 7 92 420 106 451 7.41e-57 194
MS.gene70228.t1 MTR_7g068920 31.909 351 210 6 90 418 78 421 1.64e-56 192
MS.gene70228.t1 MTR_3g035830 29.129 333 215 6 92 418 31 348 2.29e-56 190
MS.gene70228.t1 MTR_4g079700 31.445 353 211 10 76 418 32 363 2.79e-56 190
MS.gene70228.t1 MTR_3g021090 29.787 329 210 6 92 414 31 344 4.64e-56 191
MS.gene70228.t1 MTR_7g070060 30.168 358 215 10 91 421 63 412 4.98e-56 191
MS.gene70228.t1 MTR_3g071650 32.286 350 208 8 92 419 89 431 5.58e-56 191
MS.gene70228.t1 MTR_5g083260 28.406 433 266 13 19 419 5 425 7.52e-55 188
MS.gene70228.t1 MTR_5g082880 31.667 360 210 10 90 421 163 514 1.38e-54 189
MS.gene70228.t1 MTR_4g014430 30.966 352 197 10 92 414 108 442 7.79e-53 183
MS.gene70228.t1 MTR_2g091175 31.361 338 212 7 87 419 45 367 8.20e-53 181
MS.gene70228.t1 MTR_3g464570 33.451 284 156 6 156 419 3 273 9.38e-50 170
MS.gene70228.t1 MTR_3g109840 33.240 358 187 16 92 415 73 412 4.40e-46 165
MS.gene70228.t1 MTR_7g033230 30.959 365 203 12 88 418 260 609 3.66e-45 166
MS.gene70228.t1 MTR_7g033230 30.959 365 203 12 88 418 330 679 5.65e-45 166
MS.gene70228.t1 MTR_8g104970 29.670 364 205 12 90 418 118 465 1.05e-43 159
MS.gene70228.t1 MTR_5g035090 31.092 357 202 12 91 416 82 425 1.54e-43 158
MS.gene70228.t1 MTR_5g035080 31.111 360 201 14 92 418 75 420 1.59e-43 158
MS.gene70228.t1 MTR_5g035150 30.812 357 211 10 90 418 83 431 3.76e-42 154
MS.gene70228.t1 MTR_7g070060 32.365 241 151 5 91 327 63 295 9.65e-42 150
MS.gene70228.t1 MTR_4g125060 28.814 354 227 10 84 418 124 471 2.30e-41 153
MS.gene70228.t1 MTR_6g016875 31.044 364 211 10 84 418 56 408 3.19e-41 152
MS.gene70228.t1 MTR_6g016880 28.571 371 230 12 89 435 75 434 8.95e-41 150
MS.gene70228.t1 MTR_4g125060 28.814 354 227 10 84 418 163 510 9.05e-41 152
MS.gene70228.t1 MTR_4g125060 28.814 354 227 10 84 418 192 539 1.01e-40 152
MS.gene70228.t1 MTR_7g019120 29.805 359 213 14 90 421 77 423 1.68e-40 150
MS.gene70228.t1 MTR_8g027900 29.526 359 211 12 90 418 209 555 1.72e-40 152
MS.gene70228.t1 MTR_6g061710 26.613 372 228 10 85 423 160 519 2.03e-39 149
MS.gene70228.t1 MTR_6g007777 26.004 473 260 17 23 421 4 460 2.74e-38 144
MS.gene70228.t1 MTR_8g093870 29.360 344 222 11 92 418 133 472 3.00e-38 144
MS.gene70228.t1 MTR_1g076690 26.790 377 231 11 80 423 211 575 2.72e-37 143
MS.gene70228.t1 MTR_8g099300 28.969 359 212 12 91 418 73 419 5.93e-37 140
MS.gene70228.t1 MTR_5g035070 28.045 353 218 10 92 416 87 431 1.23e-35 137
MS.gene70228.t1 MTR_7g070060 27.303 304 187 8 144 421 15 310 3.03e-35 132
MS.gene70228.t1 MTR_2g016060 29.178 353 215 12 93 418 56 400 3.45e-35 135
MS.gene70228.t1 MTR_2g022580 48.780 123 63 0 152 274 2 124 1.55e-34 125
MS.gene70228.t1 MTR_4g092830 28.333 360 215 13 92 421 48 394 1.99e-33 130
MS.gene70228.t1 MTR_6g016240 28.457 376 228 16 73 418 52 416 2.84e-29 119
MS.gene70228.t1 MTR_5g035120 42.857 91 52 0 90 180 73 163 1.38e-24 100
MS.gene70228.t1 MTR_7g019140 42.857 91 52 0 90 180 77 167 9.38e-23 95.1
MS.gene70228.t1 MTR_5g069320 26.978 278 161 5 138 411 147 386 5.59e-20 91.7
MS.gene70228.t1 MTR_5g035140 28.512 242 137 12 209 418 28 265 6.69e-16 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70228.t1 AT1G48880 62.935 402 139 3 22 421 49 442 0.0 533
MS.gene70228.t1 AT5G19160 38.876 445 230 9 27 432 19 460 1.52e-101 310
MS.gene70228.t1 AT3G06080 43.785 354 178 6 92 427 103 453 4.45e-101 309
MS.gene70228.t1 AT3G62390 39.205 352 203 3 77 419 123 472 8.83e-92 286
MS.gene70228.t1 AT5G06230 38.162 359 201 5 81 420 10 366 2.92e-91 281
MS.gene70228.t1 AT5G06230 38.162 359 201 5 81 420 51 407 8.51e-91 281
MS.gene70228.t1 AT3G11570 40.741 324 181 3 108 420 98 421 3.21e-87 272
MS.gene70228.t1 AT5G19160 42.320 319 163 4 27 327 19 334 3.25e-84 262
MS.gene70228.t1 AT5G20590 37.572 346 202 6 89 421 138 482 8.68e-82 260
MS.gene70228.t1 AT3G06080 49.576 236 119 0 92 327 103 338 2.05e-81 254
MS.gene70228.t1 AT3G12060 36.963 349 205 4 89 423 193 540 3.38e-80 258
MS.gene70228.t1 AT5G06700 36.599 347 205 4 89 421 252 597 6.61e-78 253
MS.gene70228.t1 AT5G49340 37.714 350 201 6 89 422 97 445 1.85e-75 243
MS.gene70228.t1 AT3G62390 40.769 260 152 1 77 336 123 380 4.61e-72 233
MS.gene70228.t1 AT1G60790 34.493 345 208 5 92 419 190 533 1.03e-71 235
MS.gene70228.t1 AT5G06230 40.625 256 142 2 81 328 51 304 3.71e-70 224
MS.gene70228.t1 AT1G29050 32.267 375 225 7 59 418 18 378 9.14e-70 226
MS.gene70228.t1 AT2G34070 34.234 333 199 6 92 418 65 383 1.59e-65 215
MS.gene70228.t1 AT5G58600 34.000 350 195 9 92 419 66 401 6.37e-65 214
MS.gene70228.t1 AT5G20590 40.249 241 142 2 89 328 138 377 2.28e-64 212
MS.gene70228.t1 AT3G14850 34.328 335 198 6 92 418 34 354 2.61e-64 211
MS.gene70228.t1 AT1G78710 33.889 360 204 10 74 421 22 359 9.84e-64 209
MS.gene70228.t1 AT2G31110 33.718 347 209 8 79 418 30 362 1.08e-60 202
MS.gene70228.t1 AT3G11030 32.670 352 205 8 92 419 106 449 3.60e-60 203
MS.gene70228.t1 AT5G01360 30.484 351 214 7 91 418 35 378 1.36e-59 199
MS.gene70228.t1 AT2G30010 34.366 355 193 10 92 419 56 397 1.46e-59 200
MS.gene70228.t1 AT2G30010 34.366 355 193 10 92 419 99 440 3.60e-59 200
MS.gene70228.t1 AT5G01360 30.484 351 214 7 91 418 91 434 5.44e-59 199
MS.gene70228.t1 AT2G42570 30.028 353 222 6 80 421 29 367 2.07e-58 196
MS.gene70228.t1 AT3G54260 31.977 344 201 7 92 420 51 376 3.21e-58 196
MS.gene70228.t1 AT2G30900 33.235 340 199 10 92 419 43 366 1.78e-57 193
MS.gene70228.t1 AT3G55990 30.726 358 211 8 90 420 138 485 1.76e-55 191
MS.gene70228.t1 AT2G40150 30.579 363 209 8 91 420 70 422 8.60e-55 188
MS.gene70228.t1 AT1G73140 30.362 359 216 9 84 419 59 406 4.45e-54 186
MS.gene70228.t1 AT2G40320 30.769 351 212 9 92 419 81 423 1.18e-53 185
MS.gene70228.t1 AT2G40160 30.579 363 216 10 89 424 74 427 3.84e-53 184
MS.gene70228.t1 AT2G38320 28.056 360 224 8 90 422 57 408 5.83e-53 182
MS.gene70228.t1 AT2G40160 30.595 353 211 9 89 414 74 419 1.21e-52 182
MS.gene70228.t1 AT5G01620 29.558 362 222 8 76 415 96 446 3.45e-52 182
MS.gene70228.t1 AT5G01620 29.558 362 222 8 76 415 96 446 3.45e-52 182
MS.gene70228.t1 AT5G01620 29.558 362 222 8 76 415 104 454 4.21e-52 182
MS.gene70228.t1 AT5G58600 35.865 237 138 3 92 327 66 289 2.30e-50 172
MS.gene70228.t1 AT1G78710 28.977 352 179 9 74 421 15 299 1.08e-44 157
MS.gene70228.t1 AT1G01430 30.110 362 209 11 91 419 98 448 2.07e-44 161
MS.gene70228.t1 AT2G38320 27.217 327 204 7 122 422 7 325 6.42e-44 156
MS.gene70228.t1 AT1G70230 30.460 348 206 12 92 418 79 411 7.45e-43 156
MS.gene70228.t1 AT5G64470 31.564 358 206 15 92 421 55 401 1.00e-42 155
MS.gene70228.t1 AT2G31110 34.940 249 146 6 78 324 29 263 1.87e-42 151
MS.gene70228.t1 AT5G64470 31.044 364 206 15 92 421 55 407 1.32e-41 152
MS.gene70228.t1 AT2G30900 34.335 233 135 5 92 321 43 260 2.24e-41 148
MS.gene70228.t1 AT4G01080 30.618 356 198 11 91 415 90 427 6.70e-41 151
MS.gene70228.t1 AT3G02440 31.445 353 205 12 92 418 127 468 2.05e-40 150
MS.gene70228.t1 AT3G02440 31.445 353 205 12 92 418 135 476 2.14e-40 150
MS.gene70228.t1 AT4G23790 30.833 360 211 11 89 418 76 427 1.39e-39 147
MS.gene70228.t1 AT5G64020 28.771 358 218 11 92 418 57 408 2.64e-39 146
MS.gene70228.t1 AT4G11090 30.278 360 205 13 89 415 76 422 3.46e-39 146
MS.gene70228.t1 AT2G34070 30.798 263 162 6 162 418 1 249 1.06e-38 140
MS.gene70228.t1 AT3G14850 32.950 261 153 7 162 414 1 247 1.08e-38 140
MS.gene70228.t1 AT5G15890 30.226 354 208 13 92 421 187 525 1.81e-38 146
MS.gene70228.t1 AT2G40160 31.967 244 154 5 89 327 74 310 3.62e-38 140
MS.gene70228.t1 AT2G37720 27.920 351 224 10 89 418 140 482 5.89e-38 144
MS.gene70228.t1 AT4G25360 26.389 360 218 10 91 415 174 521 8.10e-38 144
MS.gene70228.t1 AT4G25360 26.389 360 218 10 91 415 174 521 8.10e-38 144
MS.gene70228.t1 AT5G20680 26.648 349 229 11 90 418 116 457 1.56e-34 134
MS.gene70228.t1 AT5G20680 27.042 355 220 12 90 418 192 533 4.33e-34 134
MS.gene70228.t1 AT5G20680 27.042 355 220 12 90 418 210 551 6.67e-34 134
MS.gene70228.t1 AT5G20680 27.042 355 220 12 90 418 210 551 6.67e-34 134
MS.gene70228.t1 AT5G20680 27.042 355 220 12 90 418 210 551 6.67e-34 134
MS.gene70228.t1 AT5G20680 27.042 355 220 12 90 418 210 551 6.67e-34 134
MS.gene70228.t1 AT5G15900 29.201 363 217 14 92 426 67 417 1.30e-33 131
MS.gene70228.t1 AT5G15900 29.201 363 217 14 92 426 86 436 1.81e-33 131
MS.gene70228.t1 AT3G28150 29.345 351 212 13 91 416 69 408 2.69e-33 130
MS.gene70228.t1 AT5G51640 25.745 369 226 9 91 423 137 493 2.57e-32 128
MS.gene70228.t1 AT2G14530 29.363 361 209 15 92 420 62 408 4.35e-32 126
MS.gene70228.t1 AT5G64470 32.129 249 145 10 92 327 55 292 6.79e-30 119
MS.gene70228.t1 AT5G64470 32.129 249 145 10 92 327 55 292 7.88e-30 118
MS.gene70228.t1 AT2G31110 32.710 214 132 5 210 418 8 214 1.07e-29 115

Find 73 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAATGTTAAGGAGTAAA+AGG 0.215471 4.1:-8460332 MS.gene70228:CDS
CATCCAAAGCGGTTGATAAT+TGG 0.219439 4.1:+8460714 None:intergenic
CACTCAAAACTTGGACTTCA+TGG 0.229773 4.1:-8459129 MS.gene70228:CDS
GTTGGAATTTCACAACTCTT+AGG 0.273880 4.1:+8460375 None:intergenic
ATAATTTCATTCCACATATC+AGG 0.293452 4.1:+8458349 None:intergenic
CAACAGGGGTTGTTATTTCC+AGG 0.328258 4.1:-8459203 MS.gene70228:intron
TAGTCTCAAAAGTGGGGAAC+TGG 0.339854 4.1:+8458551 None:intergenic
ATCAGATTACAAAGAGGATT+AGG 0.354758 4.1:-8460203 MS.gene70228:CDS
AAAGCGGTTGATAATTGGTT+TGG 0.356984 4.1:+8460719 None:intergenic
GAACAAGGATTTGATTGTTT+AGG 0.359561 4.1:-8460441 MS.gene70228:CDS
AGGAAATGGAAGAAGTGATT+TGG 0.360249 4.1:-8460421 MS.gene70228:CDS
GGAACATGGGTTCAAGTATC+TGG 0.373068 4.1:-8460501 MS.gene70228:CDS
GTAACATGGAGAACGTTTAT+AGG 0.374567 4.1:+8458466 None:intergenic
GATATTCTTATATTCAATAC+TGG 0.385489 4.1:-8460018 MS.gene70228:CDS
GGATTTGATTGTTTAGGAAA+TGG 0.407456 4.1:-8460435 MS.gene70228:CDS
AAGCGGTTGATAATTGGTTT+GGG 0.409808 4.1:+8460720 None:intergenic
AGAATGAGATCTTCTGTTGA+AGG 0.411442 4.1:+8460758 None:intergenic
GCTTTGGATGTGTTTCTTTG+AGG 0.416695 4.1:-8460701 MS.gene70228:CDS
GTGATGCACATGTTGGTAAC+TGG 0.425219 4.1:-8458423 MS.gene70228:CDS
GGAAATGTTAAGGAGTAAAA+GGG 0.425671 4.1:-8460331 MS.gene70228:CDS
ATAAAATAGCACTCAAAACT+TGG 0.427959 4.1:-8459138 MS.gene70228:CDS
AAACCAATTATCAACCGCTT+TGG 0.428304 4.1:-8460717 MS.gene70228:CDS
TCCTTCTTTATAGCAATTGG+TGG 0.441666 4.1:-8460645 MS.gene70228:CDS
TTGGAAGATCTCTGAAGCTT+GGG 0.445761 4.1:-8459180 MS.gene70228:CDS
TTGATTTGTATGTTGATGAC+TGG 0.450820 4.1:-8460264 MS.gene70228:CDS
TGCAGCCATTGGTGTCTACC+TGG 0.456267 4.1:-8458376 MS.gene70228:CDS
TTCTTTATAGCAATTGGTGG+TGG 0.458019 4.1:-8460642 MS.gene70228:CDS
AGTGTTGATTTAACCCTCCT+TGG 0.459189 4.1:+8460087 None:intergenic
TCAAATCCTTGTTCAACAAA+AGG 0.464197 4.1:+8460450 None:intergenic
GTTGGAAGATCTCTGAAGCT+TGG 0.483244 4.1:-8459181 MS.gene70228:CDS
GAATGATAATCCATTGAGTC+AGG 0.483902 4.1:-8458401 MS.gene70228:CDS
TGGCTGCAATCCTGACTCAA+TGG 0.488106 4.1:+8458391 None:intergenic
TTCAATGAGTAGAACTCAAT+GGG 0.495930 4.1:-8460292 MS.gene70228:CDS
ATTCAATGAGTAGAACTCAA+TGG 0.504721 4.1:-8460293 MS.gene70228:CDS
TTATATTCAATACTGGTCAC+TGG 0.507782 4.1:-8460011 MS.gene70228:CDS
TTCCACATATCAGGCACTCC+AGG 0.511171 4.1:+8458358 None:intergenic
AGATCTCATTCTCAACCTCT+TGG 0.514412 4.1:-8460747 MS.gene70228:CDS
TTTCTTGGTACAGCAGCGTC+CGG 0.518646 4.1:-8460124 MS.gene70228:CDS
TGAGTCAGGATTGCAGCCAT+TGG 0.524273 4.1:-8458387 MS.gene70228:CDS
ACCACCAATTGCTATAAAGA+AGG 0.526497 4.1:+8460644 None:intergenic
CTTGGTGCATGTTCATGAGC+CGG 0.529839 4.1:+8460105 None:intergenic
GGGTTACATTGCAAGTCCTA+CGG 0.531440 4.1:+8458572 None:intergenic
ACAGCACCAACATCAAAAGT+TGG 0.536992 4.1:+8460357 None:intergenic
TAGATGATATAAGTCATCAA+TGG 0.537422 4.1:-8460050 MS.gene70228:CDS
TACCTGGAGTGCCTGATATG+TGG 0.545087 4.1:-8458360 MS.gene70228:CDS
GGTTACATTGCAAGTCCTAC+GGG 0.554442 4.1:+8458573 None:intergenic
TACGTGTGGAAAAGCTTAGA+TGG 0.556442 4.1:+8459982 None:intergenic
CGTTGCATTAGTCTCAAAAG+TGG 0.557442 4.1:+8458543 None:intergenic
GTTGCATTAGTCTCAAAAGT+GGG 0.563412 4.1:+8458544 None:intergenic
AGGGGTTGTTATTTCCAGGT+TGG 0.564868 4.1:-8459199 MS.gene70228:CDS
AGAAGCTGAACTTACTGTGT+AGG 0.573237 4.1:+8458277 None:intergenic
AGTCTCAAAAGTGGGGAACT+GGG 0.574184 4.1:+8458552 None:intergenic
TATTCAATACTGGTCACTGG+TGG 0.581699 4.1:-8460008 MS.gene70228:CDS
TGGGAATACCTCCAGTGAGA+TGG 0.587027 4.1:+8459052 None:intergenic
GCTTCAGAGATCTTCCAACC+TGG 0.590103 4.1:+8459185 None:intergenic
CCTCGCAGTGATGCACATGT+TGG 0.597967 4.1:-8458430 MS.gene70228:CDS
TATGTTGATGACTGGTGTGG+AGG 0.600077 4.1:-8460256 MS.gene70228:CDS
TTGTATGTTGATGACTGGTG+TGG 0.600347 4.1:-8460259 MS.gene70228:CDS
TCATGAACATGCACCAAGGA+GGG 0.607670 4.1:-8460100 MS.gene70228:CDS
GAGCCGACATAGATGTAACA+TGG 0.615016 4.1:+8458452 None:intergenic
TCTCCATGTTACATCTATGT+CGG 0.618261 4.1:-8458455 MS.gene70228:CDS
ATTCAATACTGGTCACTGGT+GGG 0.631466 4.1:-8460007 MS.gene70228:CDS
GCACTCCAGGTAGACACCAA+TGG 0.634922 4.1:+8458371 None:intergenic
ATCAGAATCAGATTACAAAG+AGG 0.636550 4.1:-8460209 MS.gene70228:CDS
CACTCAGTTGCATTATACAA+AGG 0.639596 4.1:+8460474 None:intergenic
CTCATGAACATGCACCAAGG+AGG 0.668023 4.1:-8460101 MS.gene70228:CDS
GGCTTTGTCACAAACATGAG+TGG 0.668070 4.1:-8460804 None:intergenic
ATTGGTTTGGGACTACCAAG+AGG 0.679709 4.1:+8460732 None:intergenic
TTGCATTAGTCTCAAAAGTG+GGG 0.688555 4.1:+8458545 None:intergenic
CGGCTCATGAACATGCACCA+AGG 0.691315 4.1:-8460104 MS.gene70228:CDS
TCAAAGAAACACATCCAAAG+CGG 0.695679 4.1:+8460703 None:intergenic
GCTGCAGTGATGATACCCGT+AGG 0.703800 4.1:-8458588 MS.gene70228:intron
CCAACATGTGCATCACTGCG+AGG 0.720577 4.1:+8458430 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAACAACAACAAATA+TGG + chr4.1:8459102-8459121 None:intergenic 15.0%
!! AGAAAATATTGTTATAAAGA+AGG + chr4.1:8460249-8460268 None:intergenic 15.0%
!! AGTTGAAATATTGAAAAGAA+TGG + chr4.1:8458965-8458984 None:intergenic 20.0%
!! ATTTAAGAGAAAGACTATAA+GGG - chr4.1:8459297-8459316 MS.gene70228:intron 20.0%
!! GATATTCTTATATTCAATAC+TGG - chr4.1:8458898-8458917 MS.gene70228:intron 20.0%
!! TATTTAAGAGAAAGACTATA+AGG - chr4.1:8459296-8459315 MS.gene70228:intron 20.0%
!! TTCATATTCAGTAAAAAGTT+GGG + chr4.1:8460598-8460617 None:intergenic 20.0%
!! TTTCATATTCAGTAAAAAGT+TGG + chr4.1:8460599-8460618 None:intergenic 20.0%
!!! AGTTTTAGAGTTAATATAAC+AGG - chr4.1:8459321-8459340 MS.gene70228:intron 20.0%
! AACATAAAAAAACAGCCTTT+CGG + chr4.1:8459374-8459393 None:intergenic 25.0%
! ATAAAATAGCACTCAAAACT+TGG - chr4.1:8459778-8459797 MS.gene70228:intron 25.0%
! ATAATTTCATTCCACATATC+AGG + chr4.1:8460570-8460589 None:intergenic 25.0%
! TAGATGATATAAGTCATCAA+TGG - chr4.1:8458866-8458885 MS.gene70228:intron 25.0%
! TTGTTTATCAGTAGGTTTAA+GGG - chr4.1:8459169-8459188 MS.gene70228:CDS 25.0%
! TTTGTTTATCAGTAGGTTTA+AGG - chr4.1:8459168-8459187 MS.gene70228:CDS 25.0%
!! AAATCTGTGTTTTCATATTC+TGG - chr4.1:8459639-8459658 MS.gene70228:intron 25.0%
!! GATGATAGACACTTAAATTT+TGG - chr4.1:8459457-8459476 MS.gene70228:intron 25.0%
!! TCTAACACATTTTCTATTTC+TGG - chr4.1:8460770-8460789 MS.gene70228:CDS 25.0%
!! TTTTCCTTCTTTATAGCAAT+TGG - chr4.1:8458268-8458287 MS.gene70228:intron 25.0%
!!! AGAAAATGTGATGTTTTTGA+TGG - chr4.1:8458394-8458413 MS.gene70228:CDS 25.0%
!!! AGTTTTGAGTGCTATTTTAT+AGG + chr4.1:8459778-8459797 None:intergenic 25.0%
!!! ATCACACATGCTTTTTTTAA+TGG - chr4.1:8458319-8458338 MS.gene70228:CDS 25.0%
!!! ATGAAAACACAGATTTGTTA+AGG + chr4.1:8459635-8459654 None:intergenic 25.0%
!!! ATTTTTTCGATCTGTTTTCT+TGG - chr4.1:8458777-8458796 MS.gene70228:intron 25.0%
!!! CTGGAATCTATTTTTGTTTT+TGG - chr4.1:8459658-8459677 MS.gene70228:intron 25.0%
!!! GATCTTTTTGTTTGTTAATG+AGG - chr4.1:8459502-8459521 MS.gene70228:intron 25.0%
!!! TACTCTTTATCTCTCTTTTT+TGG + chr4.1:8460702-8460721 None:intergenic 25.0%
!!! TAGGTTCATTTTCTGAAAAA+GGG + chr4.1:8460623-8460642 None:intergenic 25.0%
!!! TGAAAACACAGATTTGTTAA+GGG + chr4.1:8459634-8459653 None:intergenic 25.0%
!!! TTGCTAATTTGTTTATCAGT+AGG - chr4.1:8459161-8459180 MS.gene70228:CDS 25.0%
AATATGAAGACTGAAGAGTA+TGG + chr4.1:8459139-8459158 None:intergenic 30.0%
ACTAAAGTTCATTAACCACT+TGG - chr4.1:8460181-8460200 MS.gene70228:CDS 30.0%
ATCAGAATCAGATTACAAAG+AGG - chr4.1:8458707-8458726 MS.gene70228:intron 30.0%
ATCAGATTACAAAGAGGATT+AGG - chr4.1:8458713-8458732 MS.gene70228:intron 30.0%
ATTATACTTGCTCAAAACAG+TGG + chr4.1:8459609-8459628 None:intergenic 30.0%
ATTCAATGAGTAGAACTCAA+TGG - chr4.1:8458623-8458642 MS.gene70228:intron 30.0%
CATCATGTTATCCATAATGT+GGG + chr4.1:8459886-8459905 None:intergenic 30.0%
GAACAAGGATTTGATTGTTT+AGG - chr4.1:8458475-8458494 MS.gene70228:CDS 30.0%
GGAAATGTTAAGGAGTAAAA+GGG - chr4.1:8458585-8458604 MS.gene70228:intron 30.0%
GGATTTGATTGTTTAGGAAA+TGG - chr4.1:8458481-8458500 MS.gene70228:CDS 30.0%
TCAAATCCTTGTTCAACAAA+AGG + chr4.1:8458469-8458488 None:intergenic 30.0%
TGGAAATGTTAAGGAGTAAA+AGG - chr4.1:8458584-8458603 MS.gene70228:CDS 30.0%
TGGTTAATGAACTTTAGTCA+AGG + chr4.1:8460179-8460198 None:intergenic 30.0%
TGTTTATCAGTAGGTTTAAG+GGG - chr4.1:8459170-8459189 MS.gene70228:CDS 30.0%
TTATATTCAATACTGGTCAC+TGG - chr4.1:8458905-8458924 MS.gene70228:intron 30.0%
TTCAATGAGTAGAACTCAAT+GGG - chr4.1:8458624-8458643 MS.gene70228:intron 30.0%
! AAAAAAGCATGTGTGATACT+AGG + chr4.1:8458316-8458335 None:intergenic 30.0%
! ACAAAGAGGATTAGGTTTTT+AGG - chr4.1:8458721-8458740 MS.gene70228:intron 30.0%
! ATCCTGACTTGAACAATTAT+TGG + chr4.1:8460127-8460146 None:intergenic 30.0%
! ATTTGGATTTTCTTTCATGG+AGG - chr4.1:8458512-8458531 MS.gene70228:CDS 30.0%
! GAAATTCCAACTTTTGATGT+TGG - chr4.1:8458553-8458572 MS.gene70228:CDS 30.0%
! GTTCTCAGTCTTTTAATAGT+TGG + chr4.1:8460675-8460694 None:intergenic 30.0%
! TCATCTTGATTTTCAAACAG+TGG + chr4.1:8459442-8459461 None:intergenic 30.0%
!! GTAGGTTCATTTTCTGAAAA+AGG + chr4.1:8460624-8460643 None:intergenic 30.0%
!! GTGATTTGGATTTTCTTTCA+TGG - chr4.1:8458509-8458528 MS.gene70228:CDS 30.0%
!! TGATGTTTTTGATGGAACAT+GGG - chr4.1:8458402-8458421 MS.gene70228:CDS 30.0%
!! TTGATTTGTATGTTGATGAC+TGG - chr4.1:8458652-8458671 MS.gene70228:intron 30.0%
!!! AGTAAAAGGGTTGTTTTTGT+TGG - chr4.1:8458598-8458617 MS.gene70228:intron 30.0%
!!! CATGCTTTTTTTAATGGACA+AGG - chr4.1:8458325-8458344 MS.gene70228:CDS 30.0%
!!! TTTGAGTGCTATTTTATAGG+CGG + chr4.1:8459775-8459794 None:intergenic 30.0%
AAACCAATTATCAACCGCTT+TGG - chr4.1:8458199-8458218 MS.gene70228:intron 35.0%
AAGTTCATTAACCACTTGGT+TGG - chr4.1:8460185-8460204 MS.gene70228:CDS 35.0%
ACATCCATTGTTCTCGTATT+AGG - chr4.1:8459241-8459260 MS.gene70228:intron 35.0%
ACCACCAATTGCTATAAAGA+AGG + chr4.1:8458275-8458294 None:intergenic 35.0%
AGAACCTAATACGAGAACAA+TGG + chr4.1:8459248-8459267 None:intergenic 35.0%
AGAATGAGATCTTCTGTTGA+AGG + chr4.1:8458161-8458180 None:intergenic 35.0%
AGGAAATGGAAGAAGTGATT+TGG - chr4.1:8458495-8458514 MS.gene70228:CDS 35.0%
ATTCAGTAAAAAGTTGGGAC+AGG + chr4.1:8460593-8460612 None:intergenic 35.0%
CACGAACTTCAAAATCATGA+CGG + chr4.1:8459271-8459290 None:intergenic 35.0%
CACTCAGTTGCATTATACAA+AGG + chr4.1:8458445-8458464 None:intergenic 35.0%
GAATGATAATCCATTGAGTC+AGG - chr4.1:8460515-8460534 MS.gene70228:CDS 35.0%
GATATGGATTCCCAAAACTA+AGG + chr4.1:8460000-8460019 None:intergenic 35.0%
GCATCATGTTATCCATAATG+TGG + chr4.1:8459887-8459906 None:intergenic 35.0%
GTAACATGGAGAACGTTTAT+AGG + chr4.1:8460453-8460472 None:intergenic 35.0%
GTTGGAATTTCACAACTCTT+AGG + chr4.1:8458544-8458563 None:intergenic 35.0%
GTTTATCAGTAGGTTTAAGG+GGG - chr4.1:8459171-8459190 MS.gene70228:CDS 35.0%
TCAAAGAAACACATCCAAAG+CGG + chr4.1:8458216-8458235 None:intergenic 35.0%
TCAAGTCAGGATTCAAATTC+AGG - chr4.1:8460135-8460154 MS.gene70228:CDS 35.0%
TCCTTCTTTATAGCAATTGG+TGG - chr4.1:8458271-8458290 MS.gene70228:intron 35.0%
TCTCCATGTTACATCTATGT+CGG - chr4.1:8460461-8460480 MS.gene70228:CDS 35.0%
TGCCAATAATTGTTCAAGTC+AGG - chr4.1:8460122-8460141 MS.gene70228:CDS 35.0%
TTCTTTATAGCAATTGGTGG+TGG - chr4.1:8458274-8458293 MS.gene70228:intron 35.0%
TTTATCAGTAGGTTTAAGGG+GGG - chr4.1:8459172-8459191 MS.gene70228:CDS 35.0%
! AAGCATGCATTCCTTAGTTT+TGG - chr4.1:8459986-8460005 MS.gene70228:CDS 35.0%
! ACTCAAACACAATTGAGTTG+TGG + chr4.1:8459071-8459090 None:intergenic 35.0%
! GTTGCATTAGTCTCAAAAGT+GGG + chr4.1:8460375-8460394 None:intergenic 35.0%
! TTGATTTTCAAACAGTGGCA+AGG + chr4.1:8459437-8459456 None:intergenic 35.0%
! TTGCATTAGTCTCAAAAGTG+GGG + chr4.1:8460374-8460393 None:intergenic 35.0%
!! AAAGCGGTTGATAATTGGTT+TGG + chr4.1:8458200-8458219 None:intergenic 35.0%
!! AAGCGGTTGATAATTGGTTT+GGG + chr4.1:8458199-8458218 None:intergenic 35.0%
!! AGCATGCATTCCTTAGTTTT+GGG - chr4.1:8459987-8460006 MS.gene70228:CDS 35.0%
!! GGATTAGGTTTTTAGGAGTT+AGG - chr4.1:8458728-8458747 MS.gene70228:intron 35.0%
!! GTGATGTTTTTGATGGAACA+TGG - chr4.1:8458401-8458420 MS.gene70228:CDS 35.0%
!!! ATAGAACACGCGTCTTTTTT+AGG - chr4.1:8459832-8459851 MS.gene70228:intron 35.0%
!!! CACTTTTGAGACTAATGCAA+CGG - chr4.1:8460374-8460393 MS.gene70228:CDS 35.0%
!!! GTGCTGTTTTGGAAATGTTA+AGG - chr4.1:8458575-8458594 MS.gene70228:CDS 35.0%
!!! TTTTGATGTTGGTGCTGTTT+TGG - chr4.1:8458564-8458583 MS.gene70228:CDS 35.0%
!!! TTTTTTTTGTCTTCACCCTC+CGG - chr4.1:8460041-8460060 MS.gene70228:CDS 35.0%
AAAGAATGGAACCTTACGTG+TGG + chr4.1:8458951-8458970 None:intergenic 40.0%
ACAGCACCAACATCAAAAGT+TGG + chr4.1:8458562-8458581 None:intergenic 40.0%
AGAAGCTGAACTTACTGTGT+AGG + chr4.1:8460642-8460661 None:intergenic 40.0%
AGATCTCATTCTCAACCTCT+TGG - chr4.1:8458169-8458188 MS.gene70228:intron 40.0%
ATCCTCTTCTTGCATTCAAC+AGG - chr4.1:8459697-8459716 MS.gene70228:intron 40.0%
ATTCAATACTGGTCACTGGT+GGG - chr4.1:8458909-8458928 MS.gene70228:intron 40.0%
CACTCAAAACTTGGACTTCA+TGG - chr4.1:8459787-8459806 MS.gene70228:intron 40.0%
GCTTATAGAATCCAACCAAG+TGG + chr4.1:8460199-8460218 None:intergenic 40.0%
TACGTGTGGAAAAGCTTAGA+TGG + chr4.1:8458937-8458956 None:intergenic 40.0%
TAGTCAAGGTCAAAACGATC+AGG + chr4.1:8460165-8460184 None:intergenic 40.0%
TATTCAATACTGGTCACTGG+TGG - chr4.1:8458908-8458927 MS.gene70228:intron 40.0%
TCCCAAAACATTTGACCGAA+AGG - chr4.1:8459356-8459375 MS.gene70228:intron 40.0%
TCCTCTTCTTGCATTCAACA+GGG - chr4.1:8459698-8459717 MS.gene70228:intron 40.0%
TCTTCTGATTTATGCTCGCT+TGG - chr4.1:8459480-8459499 MS.gene70228:intron 40.0%
! AGCCTTTCGGTCAAATGTTT+TGG + chr4.1:8459361-8459380 None:intergenic 40.0%
! CGTTGCATTAGTCTCAAAAG+TGG + chr4.1:8460376-8460395 None:intergenic 40.0%
! GAGTGTCCTTTTGTTGAACA+AGG - chr4.1:8458460-8458479 MS.gene70228:CDS 40.0%
! GCCTTTCGGTCAAATGTTTT+GGG + chr4.1:8459360-8459379 None:intergenic 40.0%
! GCTTTGGATGTGTTTCTTTG+AGG - chr4.1:8458215-8458234 MS.gene70228:intron 40.0%
! GTTTGTTAATGAGGCCTATC+AGG - chr4.1:8459511-8459530 MS.gene70228:intron 40.0%
! TCTAAGCTTTTCCACACGTA+AGG - chr4.1:8458937-8458956 MS.gene70228:intron 40.0%
! TGGATTTTCTTTCATGGAGG+TGG - chr4.1:8458515-8458534 MS.gene70228:CDS 40.0%
!! AGTGTTGATTTAACCCTCCT+TGG + chr4.1:8458832-8458851 None:intergenic 40.0%
!! CATCCAAAGCGGTTGATAAT+TGG + chr4.1:8458205-8458224 None:intergenic 40.0%
!! TTGGAAGATCTCTGAAGCTT+GGG - chr4.1:8459736-8459755 MS.gene70228:intron 40.0%
!! TTGTATGTTGATGACTGGTG+TGG - chr4.1:8458657-8458676 MS.gene70228:intron 40.0%
AAAGACCATGTCCTCACGAT+TGG + chr4.1:8459030-8459049 None:intergenic 45.0%
AACTGTAAGCCAGTAGCGTT+TGG - chr4.1:8458981-8459000 MS.gene70228:intron 45.0%
AAGACCATGTCCTCACGATT+GGG + chr4.1:8459029-8459048 None:intergenic 45.0%
AGTCTCAAAAGTGGGGAACT+GGG + chr4.1:8460367-8460386 None:intergenic 45.0%
CCTCTTCTTGCATTCAACAG+GGG - chr4.1:8459699-8459718 MS.gene70228:intron 45.0%
GAGCCGACATAGATGTAACA+TGG + chr4.1:8460467-8460486 None:intergenic 45.0%
GGAACATGGGTTCAAGTATC+TGG - chr4.1:8458415-8458434 MS.gene70228:CDS 45.0%
GGGTTACATTGCAAGTCCTA+CGG + chr4.1:8460347-8460366 None:intergenic 45.0%
GGTTACATTGCAAGTCCTAC+GGG + chr4.1:8460346-8460365 None:intergenic 45.0%
TAGTCTCAAAAGTGGGGAAC+TGG + chr4.1:8460368-8460387 None:intergenic 45.0%
TATCAGGACCCTCCTAGAAA+GGG - chr4.1:8459527-8459546 MS.gene70228:intron 45.0%
TCACTATGTCCACCCTTTCT+AGG + chr4.1:8459542-8459561 None:intergenic 45.0%
TCATGAACATGCACCAAGGA+GGG - chr4.1:8458816-8458835 MS.gene70228:intron 45.0%
TTGGGAATCCATATCGTGCA+CGG - chr4.1:8460005-8460024 MS.gene70228:CDS 45.0%
! ATGCATGCTTGCTACGTGTA+AGG + chr4.1:8459976-8459995 None:intergenic 45.0%
! CTCGTTTTCAGACACAGTGT+TGG - chr4.1:8460404-8460423 MS.gene70228:CDS 45.0%
! TATGTCCACCCTTTCTAGGA+GGG + chr4.1:8459538-8459557 None:intergenic 45.0%
!! AGGGGTTGTTATTTCCAGGT+TGG - chr4.1:8459717-8459736 MS.gene70228:intron 45.0%
!! ATTGGTTTGGGACTACCAAG+AGG + chr4.1:8458187-8458206 None:intergenic 45.0%
!! CAACAGGGGTTGTTATTTCC+AGG - chr4.1:8459713-8459732 MS.gene70228:intron 45.0%
!! CTGGAGGTATTCCCACATTA+TGG - chr4.1:8459872-8459891 MS.gene70228:intron 45.0%
!! GTGATGCACATGTTGGTAAC+TGG - chr4.1:8460493-8460512 MS.gene70228:CDS 45.0%
!! GTTGGAAGATCTCTGAAGCT+TGG - chr4.1:8459735-8459754 MS.gene70228:intron 45.0%
!! TATGTTGATGACTGGTGTGG+AGG - chr4.1:8458660-8458679 MS.gene70228:intron 45.0%
!!! AGTGCTATTTTATAGGCGGC+AGG + chr4.1:8459771-8459790 None:intergenic 45.0%
AGACCATGTCCTCACGATTG+GGG + chr4.1:8459028-8459047 None:intergenic 50.0%
AGAGAATCCCGTGCACGATA+TGG + chr4.1:8460016-8460035 None:intergenic 50.0%
CCCCTGTTGAATGCAAGAAG+AGG + chr4.1:8459702-8459721 None:intergenic 50.0%
CTATCAGGACCCTCCTAGAA+AGG - chr4.1:8459526-8459545 MS.gene70228:intron 50.0%
CTATGTCCACCCTTTCTAGG+AGG + chr4.1:8459539-8459558 None:intergenic 50.0%
CTCATGAACATGCACCAAGG+AGG - chr4.1:8458815-8458834 MS.gene70228:intron 50.0%
CTTACCTCCTAACCGAACTC+CGG + chr4.1:8460094-8460113 None:intergenic 50.0%
GCTCAAAACAGTGGCAGAGT+TGG + chr4.1:8459600-8459619 None:intergenic 50.0%
GCTTCAGAGATCTTCCAACC+TGG + chr4.1:8459734-8459753 None:intergenic 50.0%
GGTTTGCGGCTATGTGATTG+TGG - chr4.1:8459002-8459021 MS.gene70228:intron 50.0%
TAATCCGGAGTTCGGTTAGG+AGG - chr4.1:8460087-8460106 MS.gene70228:CDS 50.0%
TGAGTCAGGATTGCAGCCAT+TGG - chr4.1:8460529-8460548 MS.gene70228:CDS 50.0%
TGGCTGCAATCCTGACTCAA+TGG + chr4.1:8460528-8460547 None:intergenic 50.0%
TGGGAATACCTCCAGTGAGA+TGG + chr4.1:8459867-8459886 None:intergenic 50.0%
TGGGAATCCATATCGTGCAC+GGG - chr4.1:8460006-8460025 MS.gene70228:CDS 50.0%
TGGTAATCCGGAGTTCGGTT+AGG - chr4.1:8460084-8460103 MS.gene70228:CDS 50.0%
TTCCACATATCAGGCACTCC+AGG + chr4.1:8460561-8460580 None:intergenic 50.0%
TTTCTTGGTACAGCAGCGTC+CGG - chr4.1:8458792-8458811 MS.gene70228:intron 50.0%
! CTTGGTGCATGTTCATGAGC+CGG + chr4.1:8458814-8458833 None:intergenic 50.0%
! CTTTCTAGGAGGGTCCTGAT+AGG + chr4.1:8459528-8459547 None:intergenic 50.0%
! CTTTTGAGCCATCTCACTGG+AGG - chr4.1:8459856-8459875 MS.gene70228:intron 50.0%
! GGACTTTTGAGCCATCTCAC+TGG - chr4.1:8459853-8459872 MS.gene70228:intron 50.0%
! TACCTGGAGTGCCTGATATG+TGG - chr4.1:8460556-8460575 MS.gene70228:CDS 50.0%
!! AGCCAGTAGCGTTTGGTTTG+CGG - chr4.1:8458988-8459007 MS.gene70228:intron 50.0%
!! CGCTTTGAAACCTTGCTTGC+TGG - chr4.1:8460218-8460237 MS.gene70228:CDS 50.0%
AGCCGCAAACCAAACGCTAC+TGG + chr4.1:8458993-8459012 None:intergenic 55.0%
CCAACATGTGCATCACTGCG+AGG + chr4.1:8460489-8460508 None:intergenic 55.0%
CCTCGCAGTGATGCACATGT+TGG - chr4.1:8460486-8460505 MS.gene70228:CDS 55.0%
CGGCTCATGAACATGCACCA+AGG - chr4.1:8458812-8458831 MS.gene70228:intron 55.0%
GCACTCCAGGTAGACACCAA+TGG + chr4.1:8460548-8460567 None:intergenic 55.0%
GCTGCAGTGATGATACCCGT+AGG - chr4.1:8460328-8460347 MS.gene70228:CDS 55.0%
GGGTCTGGTAATCCGGAGTT+CGG - chr4.1:8460079-8460098 MS.gene70228:CDS 55.0%
TGATTGTGGCCCCAATCGTG+AGG - chr4.1:8459016-8459035 MS.gene70228:intron 55.0%
! GAAGGCGTAACCAGCAAGCA+AGG + chr4.1:8460231-8460250 None:intergenic 55.0%
! TGCAGCCATTGGTGTCTACC+TGG - chr4.1:8460540-8460559 MS.gene70228:CDS 55.0%
CAGGACCCTCCTAGAAAGGG+TGG - chr4.1:8459530-8459549 MS.gene70228:intron 60.0%
GCAAGGGGGGTCTGGTAATC+CGG - chr4.1:8460072-8460091 MS.gene70228:CDS 60.0%
TCTTCACCCTCCGGTTCCCA+GGG - chr4.1:8460050-8460069 MS.gene70228:CDS 60.0%
TGGCCCCAATCGTGAGGACA+TGG - chr4.1:8459022-8459041 MS.gene70228:intron 60.0%
GTCTTCACCCTCCGGTTCCC+AGG - chr4.1:8460049-8460068 MS.gene70228:CDS 65.0%
TACCAGACCCCCCTTGCCCT+GGG + chr4.1:8460069-8460088 None:intergenic 65.0%
TTACCAGACCCCCCTTGCCC+TGG + chr4.1:8460070-8460089 None:intergenic 65.0%
ACCCCCCTTGCCCTGGGAAC+CGG + chr4.1:8460063-8460082 None:intergenic 70.0%
ACCCTCCGGTTCCCAGGGCA+AGG - chr4.1:8460055-8460074 MS.gene70228:CDS 70.0%
CCCTCCGGTTCCCAGGGCAA+GGG - chr4.1:8460056-8460075 MS.gene70228:CDS 70.0%
CCCTTGCCCTGGGAACCGGA+GGG + chr4.1:8460059-8460078 None:intergenic 70.0%
CCTCCGGTTCCCAGGGCAAG+GGG - chr4.1:8460057-8460076 MS.gene70228:CDS 70.0%
CTCCGGTTCCCAGGGCAAGG+GGG - chr4.1:8460058-8460077 MS.gene70228:CDS 70.0%
TCCGGTTCCCAGGGCAAGGG+GGG - chr4.1:8460059-8460078 MS.gene70228:CDS 70.0%
TTCCCAGGGCAAGGGGGGTC+TGG - chr4.1:8460064-8460083 MS.gene70228:CDS 70.0%
CCCCTTGCCCTGGGAACCGG+AGG + chr4.1:8460060-8460079 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 8458127 8460811 8458127 ID=MS.gene70228
chr4.1 mRNA 8458127 8460811 8458127 ID=MS.gene70228.t1;Parent=MS.gene70228
chr4.1 exon 8459985 8460811 8459985 ID=MS.gene70228.t1.exon1;Parent=MS.gene70228.t1
chr4.1 CDS 8459985 8460811 8459985 ID=cds.MS.gene70228.t1;Parent=MS.gene70228.t1
chr4.1 exon 8459061 8459219 8459061 ID=MS.gene70228.t1.exon2;Parent=MS.gene70228.t1
chr4.1 CDS 8459061 8459219 8459061 ID=cds.MS.gene70228.t1;Parent=MS.gene70228.t1
chr4.1 exon 8458292 8458603 8458292 ID=MS.gene70228.t1.exon3;Parent=MS.gene70228.t1
chr4.1 CDS 8458292 8458603 8458292 ID=cds.MS.gene70228.t1;Parent=MS.gene70228.t1
chr4.1 exon 8458127 8458139 8458127 ID=MS.gene70228.t1.exon4;Parent=MS.gene70228.t1
chr4.1 CDS 8458127 8458139 8458127 ID=cds.MS.gene70228.t1;Parent=MS.gene70228.t1
Gene Sequence

>MS.gene70228

ATGAGTGGTTCACATTTGAATAAAAGCATTTCCTTCAACAGAAGATCTCATTCTCAACCTCTTGGTAGTCCCAAACCAATTATCAACCGCTTTGGATGTGTTTCTTTGAGGTTTCAAGTTCTTGTTATCATTGCTTCTGTTTTTTCCTTCTTTATAGCAATTGGTGGTGGTTACATCTATGTTCTTCCTAGTATCACACATGCTTTTTTTAATGGACAAGGTTTCTTTTCTGATCATAACAATAACAATAACAATAATTCGTTGTTGAGAAAATGTGATGTTTTTGATGGAACATGGGTTCAAGTATCTGGTTATCCTTTGTATAATGCAACTGAGTGTCCTTTTGTTGAACAAGGATTTGATTGTTTAGGAAATGGAAGAAGTGATTTGGATTTTCTTTCATGGAGGTGGAAACCTAAGAGTTGTGAAATTCCAACTTTTGATGTTGGTGCTGTTTTGGAAATGTTAAGGAGTAAAAGGGTTGTTTTTGTTGGTGATTCAATGAGTAGAACTCAATGGGAGTCTTTGATTTGTATGTTGATGACTGGTGTGGAGGATAAGACGAGTGTTTATGAAGTGAATCAGAATCAGATTACAAAGAGGATTAGGTTTTTAGGAGTTAGGTTTAGTGATTTCAATTTTACAATTGAATTTTTTCGATCTGTTTTCTTGGTACAGCAGCGTCCGGCTCATGAACATGCACCAAGGAGGGTTAAATCAACACTTTTGTTAGACAAGTTAGATGATATAAGTCATCAATGGATTGATTCAGATATTCTTATATTCAATACTGGTCACTGGTGGGTGCCATCTAAGCTTTTCCACACGTAAGGTTCCATTCTTTTCAATATTTCAACTGTAAGCCAGTAGCGTTTGGTTTGCGGCTATGTGATTGTGGCCCCAATCGTGAGGACATGGTCTTTGATTTGATGTATATGTGACCACAACTCAATTGTGTTTGAGTTGCATCAGCCATATTTGTTGTTGTTAATTTTAATGTTATTCATTGCCATACTCTTCAGTCTTCATATTTCTTGCTAATTTGTTTATCAGTAGGTTTAAGGGGGGAAAACTTTTCATCGAGTTTTCGTAACTGTTGACTTAGTAGTTCACAACATCCATTGTTCTCGTATTAGGTTCTCCGTCATGATTTTGAAGTTCGTGATTATTATTTAAGAGAAAGACTATAAGGGTAGTTTTAGAGTTAATATAACAGGTGATGCGTGAGCTCCCAAAACATTTGACCGAAAGGCTGTTTTTTTATGTTAAACTAAGTATTTCTCAAGAGATTGTTGTCGTGATTCTTTCCTTGCCACTGTTTGAAAATCAAGATGATAGACACTTAAATTTTGGTCTTCTGATTTATGCTCGCTTGGATCTTTTTGTTTGTTAATGAGGCCTATCAGGACCCTCCTAGAAAGGGTGGACATAGTGATGTTTTGTTTTTCATTCTGACTCTAACTTGAGAAACCAACTCTGCCACTGTTTTGAGCAAGTATAATCACCCTTAACAAATCTGTGTTTTCATATTCTGGAATCTATTTTTGTTTTTGGATTATTGTGTCAAATGATCCTCTTCTTGCATTCAACAGGGGTTGTTATTTCCAGGTTGGAAGATCTCTGAAGCTTGGGATGTCAATTCCTGCCGCCTATAAAATAGCACTCAAAACTTGGACTTCATGGATTGAGAGAAAGATTGATAAAAATAGAACACGCGTCTTTTTTAGGACTTTTGAGCCATCTCACTGGAGGTATTCCCACATTATGGATAACATGATGCTTTATTTACATGTGCTTAGCTTTCATTATATAATATTTTAAAATGATACTTCAAAATTCAAACTATCCTTACACGTAGCAAGCATGCATTCCTTAGTTTTGGGAATCCATATCGTGCACGGGATTCTCTTAACTTTTTTTTTGTCTTCACCCTCCGGTTCCCAGGGCAAGGGGGGTCTGGTAATCCGGAGTTCGGTTAGGAGGTAAGTAAAGTCTTGCCAATAATTGTTCAAGTCAGGATTCAAATTCAGGTTCTCCTGATCGTTTTGACCTTGACTAAAGTTCATTAACCACTTGGTTGGATTCTATAAGCGCTTTGAAACCTTGCTTGCTGGTTACGCCTTCTTTATAACAATATTTTCTTGTAACATAACATATGCTTATTTCATTGTTCAACAATTATTCATCTAGCTTTTATGTTTGCTGCAGTGATGATACCCGTAGGACTTGCAATGTAACCCAGTTCCCCACTTTTGAGACTAATGCAACGGAACAAAACTCGTTTTCAGACACAGTGTTGGAAGTTGTAAAGAATGTTAATTTTCCTATAAACGTTCTCCATGTTACATCTATGTCGGCTCCTCGCAGTGATGCACATGTTGGTAACTGGAATGATAATCCATTGAGTCAGGATTGCAGCCATTGGTGTCTACCTGGAGTGCCTGATATGTGGAATGAAATTATCCTGTCCCAACTTTTTACTGAATATGAAATCCCTTTTTCAGAAAATGAACCTACACAGTAAGTTCAGCTTCTTTCAAAATATCCAACTATTAAAAGACTGAGAACTCTGCCAAAAAAGAGAGATAAAGAGTACTTTTCTTTTACTTTCAGATGCATCATTTTAAAAAAAAATGAACTACATCTAACACATTTTCTATTTCTGGATGCAGATTAGATGACTAA
Protein sequence

>MS.gene70228.t1

MSGSHLNKSISFNRRSHSQPLGSPKPIINRFGCVSLRFQVLVIIASVFSFFIAIGGGYIYVLPSITHAFFNGQGFFSDHNNNNNNNSLLRKCDVFDGTWVQVSGYPLYNATECPFVEQGFDCLGNGRSDLDFLSWRWKPKSCEIPTFDVGAVLEMLRSKRVVFVGDSMSRTQWESLICMLMTGVEDKTSVYEVNQNQITKRIRFLGVRFSDFNFTIEFFRSVFLVQQRPAHEHAPRRVKSTLLLDKLDDISHQWIDSDILIFNTGHWWVPSKLFHTGCYFQVGRSLKLGMSIPAAYKIALKTWTSWIERKIDKNRTRVFFRTFEPSHWSDDTRRTCNVTQFPTFETNATEQNSFSDTVLEVVKNVNFPINVLHVTSMSAPRSDAHVGNWNDNPLSQDCSHWCLPGVPDMWNEIILSQLFTEYEIPFSENEPTQLDD