Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70847.t1 | XP_003617216.1 | 96.6 | 146 | 5 | 0 | 1 | 146 | 1 | 146 | 5.20E-79 | 303.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70847.t1 | Q500V5 | 32.3 | 133 | 85 | 2 | 10 | 142 | 221 | 348 | 7.3e-13 | 75.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70847.t1 | G7KCV4 | 96.6 | 146 | 5 | 0 | 1 | 146 | 1 | 146 | 3.8e-79 | 303.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05415 | MS.gene70847 | 0.815558 | 8.69E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70847.t1 | MTR_5g089140 | 96.575 | 146 | 5 | 0 | 1 | 146 | 1 | 146 | 2.98e-104 | 294 |
| MS.gene70847.t1 | MTR_5g089250 | 94.521 | 146 | 8 | 0 | 1 | 146 | 1 | 146 | 1.38e-101 | 287 |
| MS.gene70847.t1 | MTR_5g089220 | 93.151 | 146 | 10 | 0 | 1 | 146 | 1 | 146 | 3.75e-99 | 281 |
| MS.gene70847.t1 | MTR_4g079980 | 90.551 | 127 | 12 | 0 | 1 | 127 | 1 | 127 | 1.77e-81 | 237 |
| MS.gene70847.t1 | MTR_4g079830 | 61.224 | 147 | 56 | 1 | 1 | 146 | 1 | 147 | 3.69e-63 | 190 |
| MS.gene70847.t1 | MTR_3g065560 | 63.014 | 146 | 54 | 0 | 1 | 146 | 1 | 146 | 1.58e-62 | 189 |
| MS.gene70847.t1 | MTR_1g019680 | 57.778 | 135 | 41 | 1 | 1 | 135 | 1 | 119 | 6.28e-51 | 158 |
| MS.gene70847.t1 | MTR_2g011700 | 68.696 | 115 | 22 | 1 | 1 | 115 | 1 | 101 | 3.26e-50 | 157 |
| MS.gene70847.t1 | MTR_4g127390 | 58.741 | 143 | 59 | 0 | 1 | 143 | 1 | 143 | 1.23e-48 | 153 |
| MS.gene70847.t1 | MTR_1g084210 | 34.307 | 137 | 85 | 3 | 10 | 146 | 56 | 187 | 8.74e-22 | 86.7 |
| MS.gene70847.t1 | MTR_2g033390 | 32.090 | 134 | 90 | 1 | 13 | 146 | 258 | 390 | 4.57e-20 | 85.5 |
| MS.gene70847.t1 | MTR_2g033390 | 34.028 | 144 | 89 | 4 | 4 | 146 | 61 | 199 | 1.01e-18 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70847.t1 | AT1G06340 | 45.113 | 133 | 65 | 4 | 13 | 143 | 6 | 132 | 2.27e-31 | 109 |
| MS.gene70847.t1 | AT1G09320 | 35.821 | 134 | 82 | 2 | 10 | 143 | 380 | 509 | 6.48e-19 | 82.4 |
| MS.gene70847.t1 | AT1G09320 | 33.083 | 133 | 84 | 2 | 10 | 142 | 221 | 348 | 3.26e-16 | 74.7 |
| MS.gene70847.t1 | AT1G09320 | 31.206 | 141 | 84 | 5 | 11 | 142 | 40 | 176 | 8.12e-12 | 62.0 |
| MS.gene70847.t1 | AT3G06520 | 34.416 | 154 | 81 | 5 | 2 | 142 | 317 | 463 | 4.75e-15 | 71.2 |
| MS.gene70847.t1 | AT3G06520 | 30.496 | 141 | 94 | 3 | 6 | 144 | 165 | 303 | 2.68e-13 | 66.2 |
| MS.gene70847.t1 | AT3G06520 | 34.416 | 154 | 81 | 5 | 2 | 142 | 317 | 463 | 4.75e-15 | 71.2 |
| MS.gene70847.t1 | AT3G06520 | 30.496 | 141 | 94 | 3 | 6 | 144 | 165 | 303 | 2.68e-13 | 66.2 |
| MS.gene70847.t1 | AT1G26540 | 35.556 | 135 | 74 | 6 | 16 | 143 | 14 | 142 | 8.23e-14 | 67.8 |
| MS.gene70847.t1 | AT5G58610 | 35.897 | 117 | 54 | 3 | 2 | 98 | 18 | 133 | 4.87e-12 | 62.8 |
| MS.gene70847.t1 | AT5G58610 | 35.897 | 117 | 54 | 3 | 2 | 98 | 18 | 133 | 5.60e-12 | 62.8 |
| MS.gene70847.t1 | AT5G58610 | 35.897 | 117 | 54 | 3 | 2 | 98 | 18 | 133 | 5.60e-12 | 62.8 |
| MS.gene70847.t1 | AT5G58610 | 35.897 | 117 | 54 | 3 | 2 | 98 | 18 | 133 | 5.69e-12 | 62.8 |
Find 31 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTACCCTTGTGATCAGATT+AGG | 0.306305 | 5.3:-71938171 | MS.gene70847:CDS |
| TGACGAGTCGGAACTTCTTA+TGG | 0.319036 | 5.3:-71938386 | MS.gene70847:CDS |
| CTACCCTTGTGATCAGATTA+GGG | 0.322716 | 5.3:-71938170 | MS.gene70847:CDS |
| TTACGAACACGTGGTGGTAT+TGG | 0.380666 | 5.3:+71938337 | None:intergenic |
| GGGTTCATCAAGAATTGGTT+TGG | 0.383607 | 5.3:-71938150 | MS.gene70847:CDS |
| GGTTCATCAAGAATTGGTTT+GGG | 0.383908 | 5.3:-71938149 | MS.gene70847:CDS |
| GATTAGGGTTCATCAAGAAT+TGG | 0.399407 | 5.3:-71938155 | MS.gene70847:CDS |
| GGTGAAGAGAGTTGATTATA+AGG | 0.402170 | 5.3:-71938533 | MS.gene70847:CDS |
| AAGAGAGTTGATTATAAGGT+AGG | 0.406647 | 5.3:-71938529 | MS.gene70847:CDS |
| ACAATGATGGTTGGTGGGTA+GGG | 0.435977 | 5.3:-71938273 | MS.gene70847:CDS |
| TGCTAGCGAGAAGATCTTGA+TGG | 0.436352 | 5.3:-71938245 | MS.gene70847:CDS |
| AAGGGTAGTAAATAGTTTGA+TGG | 0.461531 | 5.3:+71938185 | None:intergenic |
| GAAGTATGCAGCAAAGAAGA+AGG | 0.467318 | 5.3:-71938496 | MS.gene70847:CDS |
| AGCAAAGAAGAAGGGTTTGT+AGG | 0.471858 | 5.3:-71938487 | MS.gene70847:CDS |
| GACAATGATGGTTGGTGGGT+AGG | 0.508000 | 5.3:-71938274 | MS.gene70847:CDS |
| CTTTAAGAAGATTCTTGTAA+CGG | 0.514418 | 5.3:+71938410 | None:intergenic |
| AAGAATTGGTTTGGGGAGAG+TGG | 0.520072 | 5.3:-71938141 | MS.gene70847:CDS |
| TCTCTTCACCGGTGGACGCA+TGG | 0.520892 | 5.3:+71938546 | None:intergenic |
| TTGTGCAACCATGCGTCCAC+CGG | 0.536842 | 5.3:-71938554 | None:intergenic |
| ATTGTTTCATGTCTTGAGAG+TGG | 0.540396 | 5.3:-71938445 | MS.gene70847:CDS |
| TGATCGAGTTCAAACTTCGA+AGG | 0.551060 | 5.3:+71938313 | None:intergenic |
| TTGGGGAGAGTGGATCTTCG+AGG | 0.562034 | 5.3:-71938131 | MS.gene70847:CDS |
| TTATAATCAACTCTCTTCAC+CGG | 0.566683 | 5.3:+71938535 | None:intergenic |
| AAGTATGCAGCAAAGAAGAA+GGG | 0.604561 | 5.3:-71938495 | MS.gene70847:CDS |
| TAATCAACTCTCTTCACCGG+TGG | 0.623258 | 5.3:+71938538 | None:intergenic |
| TGAACCCTAATCTGATCACA+AGG | 0.626143 | 5.3:+71938166 | None:intergenic |
| GTTCATCAAGAATTGGTTTG+GGG | 0.626950 | 5.3:-71938148 | MS.gene70847:CDS |
| TCTTCTTAAAGATGACGAGT+CGG | 0.627366 | 5.3:-71938398 | MS.gene70847:CDS |
| GAAGGATTACGAACACGTGG+TGG | 0.672554 | 5.3:+71938331 | None:intergenic |
| TTCGAAGGATTACGAACACG+TGG | 0.704792 | 5.3:+71938328 | None:intergenic |
| GAACCCTAATCTGATCACAA+GGG | 0.736467 | 5.3:+71938167 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTTAAGAAGATTCTTGTAA+CGG | + | chr5.3:71938263-71938282 | None:intergenic | 25.0% |
| AAGGGTAGTAAATAGTTTGA+TGG | + | chr5.3:71938488-71938507 | None:intergenic | 30.0% | |
| TTATAATCAACTCTCTTCAC+CGG | + | chr5.3:71938138-71938157 | None:intergenic | 30.0% | |
| ! | AAGAGAGTTGATTATAAGGT+AGG | - | chr5.3:71938141-71938160 | MS.gene70847:CDS | 30.0% |
| !! | ATGTTTTTGACAATGATGGT+TGG | - | chr5.3:71938388-71938407 | MS.gene70847:CDS | 30.0% |
| !! | GTTGATGTTTTTGACAATGA+TGG | - | chr5.3:71938384-71938403 | MS.gene70847:CDS | 30.0% |
| AAGTATGCAGCAAAGAAGAA+GGG | - | chr5.3:71938175-71938194 | MS.gene70847:CDS | 35.0% | |
| ATTGTTTCATGTCTTGAGAG+TGG | - | chr5.3:71938225-71938244 | MS.gene70847:CDS | 35.0% | |
| GATTAGGGTTCATCAAGAAT+TGG | - | chr5.3:71938515-71938534 | MS.gene70847:CDS | 35.0% | |
| TCTTCTTAAAGATGACGAGT+CGG | - | chr5.3:71938272-71938291 | MS.gene70847:CDS | 35.0% | |
| ! | GGTGAAGAGAGTTGATTATA+AGG | - | chr5.3:71938137-71938156 | MS.gene70847:CDS | 35.0% |
| ! | GGTTCATCAAGAATTGGTTT+GGG | - | chr5.3:71938521-71938540 | MS.gene70847:CDS | 35.0% |
| ! | TTTTGACAATGATGGTTGGT+GGG | - | chr5.3:71938392-71938411 | MS.gene70847:CDS | 35.0% |
| ! | TTTTTGACAATGATGGTTGG+TGG | - | chr5.3:71938391-71938410 | MS.gene70847:CDS | 35.0% |
| !! | GTTCATCAAGAATTGGTTTG+GGG | - | chr5.3:71938522-71938541 | MS.gene70847:CDS | 35.0% |
| ACTACCCTTGTGATCAGATT+AGG | - | chr5.3:71938499-71938518 | MS.gene70847:CDS | 40.0% | |
| AGCAAAGAAGAAGGGTTTGT+AGG | - | chr5.3:71938183-71938202 | MS.gene70847:CDS | 40.0% | |
| CTACCCTTGTGATCAGATTA+GGG | - | chr5.3:71938500-71938519 | MS.gene70847:CDS | 40.0% | |
| GAACCCTAATCTGATCACAA+GGG | + | chr5.3:71938506-71938525 | None:intergenic | 40.0% | |
| GAAGTATGCAGCAAAGAAGA+AGG | - | chr5.3:71938174-71938193 | MS.gene70847:CDS | 40.0% | |
| GGGTTCATCAAGAATTGGTT+TGG | - | chr5.3:71938520-71938539 | MS.gene70847:CDS | 40.0% | |
| TGAACCCTAATCTGATCACA+AGG | + | chr5.3:71938507-71938526 | None:intergenic | 40.0% | |
| TGATCGAGTTCAAACTTCGA+AGG | + | chr5.3:71938360-71938379 | None:intergenic | 40.0% | |
| TAATCAACTCTCTTCACCGG+TGG | + | chr5.3:71938135-71938154 | None:intergenic | 45.0% | |
| TGACGAGTCGGAACTTCTTA+TGG | - | chr5.3:71938284-71938303 | MS.gene70847:CDS | 45.0% | |
| TGCTAGCGAGAAGATCTTGA+TGG | - | chr5.3:71938425-71938444 | MS.gene70847:CDS | 45.0% | |
| TTACGAACACGTGGTGGTAT+TGG | + | chr5.3:71938336-71938355 | None:intergenic | 45.0% | |
| TTCGAAGGATTACGAACACG+TGG | + | chr5.3:71938345-71938364 | None:intergenic | 45.0% | |
| !! | AAGAATTGGTTTGGGGAGAG+TGG | - | chr5.3:71938529-71938548 | MS.gene70847:CDS | 45.0% |
| !! | ACAATGATGGTTGGTGGGTA+GGG | - | chr5.3:71938397-71938416 | MS.gene70847:CDS | 45.0% |
| GAAGGATTACGAACACGTGG+TGG | + | chr5.3:71938342-71938361 | None:intergenic | 50.0% | |
| !! | GACAATGATGGTTGGTGGGT+AGG | - | chr5.3:71938396-71938415 | MS.gene70847:CDS | 50.0% |
| TTGGGGAGAGTGGATCTTCG+AGG | - | chr5.3:71938539-71938558 | MS.gene70847:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 71938126 | 71938566 | 71938126 | ID=MS.gene70847 |
| chr5.3 | mRNA | 71938126 | 71938566 | 71938126 | ID=MS.gene70847.t1;Parent=MS.gene70847 |
| chr5.3 | exon | 71938126 | 71938566 | 71938126 | ID=MS.gene70847.t1.exon1;Parent=MS.gene70847.t1 |
| chr5.3 | CDS | 71938126 | 71938566 | 71938126 | ID=cds.MS.gene70847.t1;Parent=MS.gene70847.t1 |
| Gene Sequence |
| Protein sequence |
